LOCUS       DANKHK010000001        83099 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000001 DANKHK010000000
VERSION     DANKHK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(193..1689)
                     /gene="lysS"
                     /locus_tag="PW109_000001"
     CDS             complement(193..1689)
                     /gene="lysS"
                     /locus_tag="PW109_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2509887.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1800..2804)
                     /gene="dusB"
                     /locus_tag="PW109_000002"
     CDS             complement(1800..2804)
                     /gene="dusB"
                     /locus_tag="PW109_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2509888.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2805..3692)
                     /gene="hslO"
                     /locus_tag="PW109_000003"
     CDS             complement(2805..3692)
                     /gene="hslO"
                     /locus_tag="PW109_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2509889.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3909..6020)
                     /gene="ftsH"
                     /locus_tag="PW109_000004"
     CDS             complement(3909..6020)
                     /gene="ftsH"
                     /locus_tag="PW109_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2509890.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6110..6655)
                     /gene="hpt"
                     /locus_tag="PW109_000005"
     CDS             complement(6110..6655)
                     /gene="hpt"
                     /locus_tag="PW109_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2509891.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6655..8100)
                     /gene="tilS"
                     /locus_tag="PW109_000006"
     CDS             complement(6655..8100)
                     /gene="tilS"
                     /locus_tag="PW109_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2509892.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8220..8690)
                     /locus_tag="PW109_000007"
     CDS             complement(8220..8690)
                     /locus_tag="PW109_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2509893.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8736..9161)
                     /locus_tag="PW109_000008"
     CDS             complement(8736..9161)
                     /locus_tag="PW109_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2509894.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9228..9497)
                     /locus_tag="PW109_000009"
     CDS             complement(9228..9497)
                     /locus_tag="PW109_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2509895.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9508..11103)
                     /locus_tag="PW109_000010"
     CDS             complement(9508..11103)
                     /locus_tag="PW109_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2509896.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11118..14639)
                     /gene="mfd"
                     /locus_tag="PW109_000011"
     CDS             complement(11118..14639)
                     /gene="mfd"
                     /locus_tag="PW109_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2509897.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14656..15216)
                     /locus_tag="PW109_000012"
     CDS             complement(14656..15216)
                     /locus_tag="PW109_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2509898.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15569..16543
                     /locus_tag="PW109_000013"
     CDS             15569..16543
                     /locus_tag="PW109_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2509899.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16713..18113
                     /locus_tag="PW109_000014"
     CDS             16713..18113
                     /locus_tag="PW109_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2509900.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18191..18913)
                     /locus_tag="PW109_000015"
     CDS             complement(18191..18913)
                     /locus_tag="PW109_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2509901.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18992..19498)
                     /locus_tag="PW109_000016"
     CDS             complement(18992..19498)
                     /locus_tag="PW109_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2509902.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19621..21420
                     /locus_tag="PW109_000017"
     CDS             19621..21420
                     /locus_tag="PW109_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2509903.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKASLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21488..23062)
                     /locus_tag="PW109_000018"
     CDS             complement(21488..23062)
                     /locus_tag="PW109_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2509904.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23317..23826)
                     /gene="mreD"
                     /locus_tag="PW109_000019"
     CDS             complement(23317..23826)
                     /gene="mreD"
                     /locus_tag="PW109_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2509905.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23830..24684)
                     /gene="mreC"
                     /locus_tag="PW109_000020"
     CDS             complement(23830..24684)
                     /gene="mreC"
                     /locus_tag="PW109_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2509906.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24839..25477)
                     /locus_tag="PW109_000021"
     CDS             complement(24839..25477)
                     /locus_tag="PW109_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2509907.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25478..26128)
                     /locus_tag="PW109_000022"
     CDS             complement(25478..26128)
                     /locus_tag="PW109_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2509908.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26141..27040)
                     /gene="rsgA"
                     /locus_tag="PW109_000023"
     CDS             complement(26141..27040)
                     /gene="rsgA"
                     /locus_tag="PW109_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2509909.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27205..29274)
                     /gene="pknB"
                     /locus_tag="PW109_000024"
     CDS             complement(27205..29274)
                     /gene="pknB"
                     /locus_tag="PW109_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2509910.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29271..30011)
                     /locus_tag="PW109_000025"
     CDS             complement(29271..30011)
                     /locus_tag="PW109_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2509911.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30024..31382)
                     /gene="rsmB"
                     /locus_tag="PW109_000026"
     CDS             complement(30024..31382)
                     /gene="rsmB"
                     /locus_tag="PW109_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2509912.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31382..32320)
                     /locus_tag="PW109_000027"
     CDS             complement(31382..32320)
                     /locus_tag="PW109_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2509913.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32313..32804)
                     /gene="def"
                     /locus_tag="PW109_000028"
     CDS             complement(32313..32804)
                     /gene="def"
                     /locus_tag="PW109_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2509914.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32819..35227)
                     /gene="priA"
                     /locus_tag="PW109_000029"
     CDS             complement(32819..35227)
                     /gene="priA"
                     /locus_tag="PW109_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2509915.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35480..35785)
                     /locus_tag="PW109_000030"
     CDS             complement(35480..35785)
                     /locus_tag="PW109_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2509916.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35790..36404)
                     /gene="gmk"
                     /locus_tag="PW109_000031"
     CDS             complement(35790..36404)
                     /gene="gmk"
                     /locus_tag="PW109_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2509917.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36891..37175)
                     /locus_tag="PW109_000032"
     CDS             complement(36891..37175)
                     /locus_tag="PW109_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509918.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37193..38083)
                     /locus_tag="PW109_000033"
     CDS             complement(37193..38083)
                     /locus_tag="PW109_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2509919.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38103..38321)
                     /locus_tag="PW109_000034"
     CDS             complement(38103..38321)
                     /locus_tag="PW109_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2509920.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38291..39991)
                     /locus_tag="PW109_000035"
     CDS             complement(38291..39991)
                     /locus_tag="PW109_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2509921.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40258..41031
                     /locus_tag="PW109_000036"
     CDS             40258..41031
                     /locus_tag="PW109_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509922.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41112..41681)
                     /gene="thiT"
                     /locus_tag="PW109_000037"
     CDS             complement(41112..41681)
                     /gene="thiT"
                     /locus_tag="PW109_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2509923.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41747..41849)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41855..42826)
                     /locus_tag="PW109_000038"
     CDS             complement(41855..42826)
                     /locus_tag="PW109_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2509924.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42920..44293)
                     /gene="glmU"
                     /locus_tag="PW109_000039"
     CDS             complement(42920..44293)
                     /gene="glmU"
                     /locus_tag="PW109_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2509925.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44796..45614)
                     /gene="purR"
                     /locus_tag="PW109_000040"
     CDS             complement(44796..45614)
                     /gene="purR"
                     /locus_tag="PW109_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2509926.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45781..46605)
                     /locus_tag="PW109_000041"
     CDS             complement(45781..46605)
                     /locus_tag="PW109_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2509927.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46578..47258)
                     /locus_tag="PW109_000042"
     CDS             complement(46578..47258)
                     /locus_tag="PW109_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2509928.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47281..48219)
                     /locus_tag="PW109_000043"
     CDS             complement(47281..48219)
                     /locus_tag="PW109_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2509929.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48464..49327)
                     /locus_tag="PW109_000044"
     CDS             complement(48464..49327)
                     /locus_tag="PW109_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2509930.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49469..52042)
                     /gene="mprF"
                     /locus_tag="PW109_000045"
     CDS             complement(49469..52042)
                     /gene="mprF"
                     /locus_tag="PW109_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2509931.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52183..52989
                     /gene="proC"
                     /locus_tag="PW109_000046"
     CDS             52183..52989
                     /gene="proC"
                     /locus_tag="PW109_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2509932.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53068..54624)
                     /gene="rny"
                     /locus_tag="PW109_000047"
     CDS             complement(53068..54624)
                     /gene="rny"
                     /locus_tag="PW109_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2509933.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55035..56084)
                     /gene="recA"
                     /locus_tag="PW109_000048"
     CDS             complement(55035..56084)
                     /gene="recA"
                     /locus_tag="PW109_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2509934.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56193..57434)
                     /locus_tag="PW109_000049"
     CDS             complement(56193..57434)
                     /locus_tag="PW109_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2509935.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57674..58252)
                     /gene="pgsA"
                     /locus_tag="PW109_000050"
     CDS             complement(57674..58252)
                     /gene="pgsA"
                     /locus_tag="PW109_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2509936.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58461..59384)
                     /locus_tag="PW109_000051"
     CDS             complement(58461..59384)
                     /locus_tag="PW109_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2509937.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60123..61409)
                     /locus_tag="PW109_000052"
     CDS             complement(60123..61409)
                     /locus_tag="PW109_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2509938.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61402..62670)
                     /locus_tag="PW109_000053"
     CDS             complement(61402..62670)
                     /locus_tag="PW109_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2509939.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62922..63383
                     /gene="mscL"
                     /locus_tag="PW109_000054"
     CDS             62922..63383
                     /gene="mscL"
                     /locus_tag="PW109_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2509940.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63442..63888)
                     /locus_tag="PW109_000055"
     CDS             complement(63442..63888)
                     /locus_tag="PW109_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2509941.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63981..64226)
                     /locus_tag="PW109_000056"
     CDS             complement(63981..64226)
                     /locus_tag="PW109_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2509942.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64341..64919)
                     /locus_tag="PW109_000057"
     CDS             complement(64341..64919)
                     /locus_tag="PW109_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2509943.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64996..65526)
                     /locus_tag="PW109_000058"
     CDS             complement(64996..65526)
                     /locus_tag="PW109_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2509944.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65530..66390)
                     /gene="rlmB"
                     /locus_tag="PW109_000059"
     CDS             complement(65530..66390)
                     /gene="rlmB"
                     /locus_tag="PW109_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2509945.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66383..66778)
                     /locus_tag="PW109_000060"
     CDS             complement(66383..66778)
                     /locus_tag="PW109_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2509946.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66775..68187)
                     /locus_tag="PW109_000061"
     CDS             complement(66775..68187)
                     /locus_tag="PW109_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2509947.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68184..68720)
                     /gene="cysE"
                     /locus_tag="PW109_000062"
     CDS             complement(68184..68720)
                     /gene="cysE"
                     /locus_tag="PW109_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2509948.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68806..69045)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(69042..70502)
                     /locus_tag="PW109_000063"
     CDS             complement(69042..70502)
                     /locus_tag="PW109_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2509949.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70672..71829)
                     /locus_tag="PW109_000064"
     CDS             complement(70672..71829)
                     /locus_tag="PW109_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2509950.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71863..73233)
                     /gene="radA"
                     /locus_tag="PW109_000065"
     CDS             complement(71863..73233)
                     /gene="radA"
                     /locus_tag="PW109_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2509951.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73296..73823)
                     /locus_tag="PW109_000066"
     CDS             complement(73296..73823)
                     /locus_tag="PW109_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2509952.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            74053..74859
                     /gene="proB"
                     /locus_tag="PW109_000067"
     CDS             74053..74859
                     /gene="proB"
                     /locus_tag="PW109_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2509953.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74856..76103
                     /locus_tag="PW109_000068"
     CDS             74856..76103
                     /locus_tag="PW109_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2509954.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76179..76523
                     /locus_tag="PW109_000069"
     CDS             76179..76523
                     /locus_tag="PW109_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509955.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76640..76966)
                     /locus_tag="PW109_000070"
     CDS             complement(76640..76966)
                     /locus_tag="PW109_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509956.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77581..78783)
                     /locus_tag="PW109_000071"
     CDS             complement(77581..78783)
                     /locus_tag="PW109_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2509957.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78900..79415
                     /locus_tag="PW109_000072"
     CDS             78900..79415
                     /locus_tag="PW109_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2509958.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79614..80060)
                     /locus_tag="PW109_000073"
     CDS             complement(79614..80060)
                     /locus_tag="PW109_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2509959.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(80107..81585)
                     /gene="msr(C)"
                     /locus_tag="PW109_000074"
     CDS             complement(80107..81585)
                     /gene="msr(C)"
                     /locus_tag="PW109_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2509960.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81939..82568)
                     /locus_tag="PW109_000075"
     CDS             complement(81939..82568)
                     /locus_tag="PW109_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2509961.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(82552..83016)
                     /locus_tag="PW109_000076"
     CDS             complement(82552..83016)
                     /locus_tag="PW109_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2509962.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        23036 a        18030 c        14883 g        27150 t
ORIGIN      
        1 caacgaattt tttgtatttt ttcaagtttt tttcaataaa cgattattca taacttttct
       61 ttctttatcg ttcgttttcg tcttctttaa tgtctagaat agtcggcatt agcttttgaa
      121 ctttcgttat tatttattcc tttctacaca gcaaaagctg cctaaggaat tccttagaca
      181 gcttttattc gattatctca ttgttgggaa tagcaaaaca tcccgaattg attgtgcatc
      241 agttagcaac attactaatc tgtcgatccc gatgcctaga ccaccagttg gcggcatacc
      301 gtattctaag gcttccaaga agtcttcgtc tacaccgtga gcttcgtcat ttcctagttc
      361 acgttctttt tcttgcgctt cgaatctttc tctttgatca attggatcgt tcagttctgt
      421 aaaggcatta gcaaattcgc gccccactat gaatagttcg aatcgatctg tgaatcgagg
      481 atcttctgga tttttcttcg ctaaaggcga aacttcgact ggatgtccgt atacaaacgt
      541 cggttgtgtc aatgtttctt caacaaatgt ttcaaagaat tcattgataa catgtccgac
      601 acccatagct tcggttactt ctacattgtg tttttcggct aaagctaatg cttcttccaa
      661 tgtcatttct tgccagaagt ctacaccagt ttgttctttg attgcatcta ccatatggat
      721 gcgtttaaag tcactttcta agtcgacagc ttgtccatca tatgtgattt ttgctgttcc
      781 tagtactttt tcagctgcgt tacggataat accttctgtc agattcatta catcattgaa
      841 atcagtatat gctgtgtatg cttctaacat cgtaaattct ggattatgtg tagtatcaat
      901 tccttcatta cggaaaacgc ggccgatttc ataaactttt tccattccgc caacaattag
      961 tcgttttaaa tgaagttcta gcgcaatacg caaatataaa tcaatgtcca gtgcattatg
     1021 atgcgtgata aatgggcgtg cggcagctcc acctgcctca ttgtgcaata ctggtgtttc
     1081 tacttcaata tagccattgc catctaaata gcgacggatt tcgctgataa tttggcttcg
     1141 tttcataaat cggtcaaagc tttcgcgatt actgattaaa tccaagtagc gttgtctgta
     1201 gcgttgttca atattcgtta agccatggta tttatctggc agtggacgaa gtgcttttga
     1261 caaaatcgtg atttctgaag ctttgataga aacttcgcct gtgtctgttt tcatgatttg
     1321 tccagttaca ccaaagaaat ctccaaggtc tgcatgtttg aatacttcat aggcctcatc
     1381 acctacttgg tcttttctta cataaatttg aatttggcct tcacgatctt gtagatgcgc
     1441 aaatccagct ttcccttttc ctcggcgagt catcatacga ccagctacac tagcggtcaa
     1501 tcccatttct gctagttctt cttttgttct tggatcaaat agttcatgca attcttctga
     1561 gttgtgcgtg cgttcaaatc gttttccaaa tggatcgatt ccatcttcac gcaattgttc
     1621 cattttttga cgacgtacaa gcatttgatc atttaaatct tctacttgat gttgttgttc
     1681 ctgtgtcaca aaagttcctc cattctcatg ttaatctctt tcctataatg ccaatgaaac
     1741 acaaaaaaag caagccgaac ttggaatttc ggcttgaaaa ttcacgaaaa acttagaaat
     1801 cacaacgttt gtttcaatag tactttttcc gctttttcta caaattgatc cagcaggtca
     1861 ttgatttctt gctgtttttc tgtctgattg attgctactt tcactttcgc tgctcgagga
     1921 atccctttta aataataagc cgcatgtgtt ctgaattctc tacaagctac tttttctcct
     1981 ttgagatctg ctaagcgttg taaatgaagt ttagctgtag caattttttc ggctggtgtt
     2041 ggttcaggaa tcagttcccc tgtttcgaga taatgctgtg tgcggtgaat catccatgga
     2101 ttgcccagcg ctgcacgacc aatcatcaca ccgtctgcac cgacatagtc aagcatccat
     2161 tttgcatctt ctggcgtttt gacatccccg ttccccataa atggaatcgt caaatgtttt
     2221 ttcacttctt tcaatacttc ccagtccgct tttccttcat acatctgaac ccgtgtacgt
     2281 ccatgcatag cgatggcact tgctccagct gattgtgctg ctaatgcatt ttctacagca
     2341 aagacgtgct cttcatccca accaatacgc atcttgattg ttactgggat atttactgct
     2401 gaagaaacag cttggaccat ttcgtatact ttctcaggat ctaaaagcca ttttgctcct
     2461 gcttctgctt tgattacttt atttactgga cagcccatat tgatatctat tatatcggca
     2521 gctgtgtttt cttcgacaaa ttgagcagct tcaactagac tatctttatt tccgccaaat
     2581 atctgtaaac ttaatggatg ttctgtttct tcaatgtgga gcatctctaa agtttttttg
     2641 tttctgaaat gaataccttg gtcactgatc atttcacata cgactaagcc cgcaccaaat
     2701 tctttgacag tgactcgaaa tgctgaattt gttattccag ccatcggtgc aacaacgaca
     2761 cgattaggga tttccacatt gccaattttc catgtgtgtt ccacttaaat ccctcctaag
     2821 gcttctttct tcaatccatc taattcttct tcgctatact tgtatttgtt attacagaaa
     2881 gaacaaaccg cttctgcacc gtgatcttcg tctatcattg cttggatttc ttctgcacct
     2941 aacgcgataa ttgcttctgc aaatttttct tttgaacagt cacatttgaa ttgaacaggc
     3001 attttttcca aaatgttcaa ttcttctgta ccaagcaatc ctttcaatat ttcttctggt
     3061 gtctgtcctt catctaagag ggtagagatt cttggtgtcg tttttaattg ttcttcgatc
     3121 ttgctgattg tttcttcatc ggcacctggc atgatttgga tcatgaatcc tcccgccgtc
     3181 ttgatgctgt cgtcagtatt gaccaataca ctcagtccaa cagctgaagg gatttgttct
     3241 gagatagcaa gatagtaagt aaaatcttca gcgatttctc cagaaacaat cggcgtttgc
     3301 ccagaaaaag gttcttttaa tcctaagtct tttgtgacag taaacattcc ttgtgttcca
     3361 actgctcccc gcacatcgat tttaccttca ctatttagag gaagactgag atgcggattt
     3421 ttgatgtaac ctttcacatc tccctttcca tttgcatcta ctacaattcc tgttgctgga
     3481 ccatcgcctt ggattcgaac agttagtttg tcttctcctt tgagcgtcgc accaagtagc
     3541 agactaccga ccaatgtccg tcccaaagca gctgaggagg tgtgccaagt atcatgacgt
     3601 ctttgtgcct ccgctacggt atttgttgct tgaacggcgt atgctcggac gaatccgtcg
     3661 tgagcaagtg ccttaaccaa ataatctttc atttgttatg tactcctttt tccgttttta
     3721 aactcatagt gaatcatact agcaactact gcttttttca actttggaat gcttctttta
     3781 aaaataattc agaatcatct agaaaacagg acatcaccct atttacaact taaataaaga
     3841 aatctcccga aaaccggttt ttgttccggt tttcgggagt catatcagat caattgacct
     3901 atattttttt atttgtattg atcgtctgaa tgattatctt ctgattgttg agaatctgaa
     3961 tcatgttctg ctttattttc ctcattcagt tcatgttttg cttcttcaaa atcacgtttt
     4021 tcttccgctt gtttttcagc gtctttttct tccaaagcac gttttgcttc ttcaaaagtt
     4081 ttcgcttctt tttcgcttgg ataatcattt gactcagtac cttcaggcat cacaccgtgt
     4141 tcaaacaatg acttgattgc tttggcatcc aatgtttcga attccaaaag tttttcagca
     4201 atcagtttat gctgtgcacg atgagcttcg atgatttctc gagctttatc atgagcttcc
     4261 atcaagattt ttcgtacttc ttgatcaatc tcgaaagcga cttgttccga gtaagctttt
     4321 gtttgaccat agtcgcggcc aacaaacact tgatggtttc cttcatattg aacaggacct
     4381 aaacgatcac tcattccata ttctgttacc atgcttcgtg ctagagctgt cgcttgctca
     4441 aagtcattgg aagcaccagt tgattggaca ttgaagatga tttcttcagc tgtacgtcca
     4501 ccaagtaagc ctacgatttg ttcaaacata tcttctttcg tcatcaagaa ctgatcttct
     4561 tttggtaaag cgatcatata tccgccggcg cgtccgcgag ggatgatcgt tactttgtgg
     4621 acgacccgag cgcgactcaa tactaatccg acaatcgtat gaccagcttc gtgataagca
     4681 accatttcgc gttctttttt gctgattacg cgatcttttt tagctggacc tgcaatcacg
     4741 cgatcttccg cttcatcgat atcagaagca tcgattttct ttttatttct tctagcagct
     4801 actaacgctg cttcattcaa gacattttct aaatctgctc cggcaaaacc tggtgtctgt
     4861 tgagccacaa ctttcaagtc aacgtcatct gccatcggtt tattccgtgc atggactcgc
     4921 aagattgctt cacgaccttt tacatctgga cgtccaacta aaatctggcg gtcaaaacgg
     4981 cctggacgta gtaacgctgg gtctagtaca tcagaacggt tggttgcagc aatgacgatt
     5041 actccttcat ttccgtcgaa gccatccatt tctacaagca attggttcaa tgtttgttcg
     5101 cgttcatcat gtccgccgcc cataccggca ccacgttgac gacctaccgc atcaatttca
     5161 tcaatgaaaa tgatagctgg tgcgtttttc ttcgctgttt caaacaaatc acgaacacga
     5221 cttgctccaa caccgacaaa catttctacg aaatcagaac ccgagatcga gtaaaacggt
     5281 actccagctt ctccggcaac agcttttgct aataaggttt tacctgttcc tggaggtcct
     5341 tctaaaagta cacctgccgg gatacgggca cctaattcaa caaatcggcg aggatctttc
     5401 agaaattcaa caacttcaac tagttcttgt ttttcttctt ccgcacccgc tacatcagaa
     5461 aaacgtacgc gattagcttt tttgtctgct tctttggctt ttgatttacc aaagttcatg
     5521 acacggccgc caccgccacc gccgccgcct tgttgtccca tcatcatgta aaagaaaaag
     5581 atgatgatta cgattggcaa gaagctaagc aatagggaaa cccatatacc gctgcttgat
     5641 tcttctttta ctgttgtagc aactttgttt tcttttgcca gttctgttac ttggctcaat
     5701 gttgtatcat ttggcaaaat gattgttgtg aaatgggtag tagtcgtatc tgttgaacct
     5761 aagatagcta acccaccgct gttagcgacg gtctgctgtt ctttgtattc accagtgatc
     5821 ttatatacac catttgcagg ctgcacttca aattctttga tcttgcctgc ttgtagttgt
     5881 tctgtgaatt ttgagtactc gatatctgac gatcgctggc cgttattacc aaaaatgaaa
     5941 taaacgacca ttaccatcgc taagattacg aggacataat acaaggcatt tttcatcatg
     6001 ccgttgttct ttttgttcat agttgccctc cctaagcgtc aatttaattt aacagttttt
     6061 tatcaatcct gaccattcta ttgtaccata atttaattga gatacagcat taatttgatt
     6121 gataaacact aggtttcaat acaccgatgt acggaaggtt acgatatgct tctgcataat
     6181 ccaagccata accgaccaca aattcatttg gtacgttgaa gccaacataa tctgcttcga
     6241 tatttactac tcttccttca ggtttgtcaa gtagtgtaac aattttgact gattttgctt
     6301 tgcggtattt gaaaagatcc actaaataag caagtgttct tccgctgtca atgatatctt
     6361 caacgatcaa cagatcgcga ccttctacat tcgtatctaa gtctttgacg atttttactt
     6421 cccctgacga gactgttgca ttcccgtagc ttgaaacatc catgaaatcc aattccaaat
     6481 acgtatcgat agaacgaacg atatctgcca tgaatggtac cgctcctttt aagacaccta
     6541 ctactagggg attggtgttt tggtaaattt ctgttaactc tttgcctagt tctttgcaac
     6601 gaacttgtat ctcttcttgt gagatcaaaa ttctctcaat atccttttcc aacatcaggt
     6661 tctccttcca attgcttctt tttgatattt gtaaagaagt atgtagtgta ttttatcagt
     6721 ttcttcacga atactcaaat atgaaaaggc aaaaggtatc aagctatata tatttctttt
     6781 ggcatctgta atgatccaag catgctgcct ttgtgaatta ggtactttct gatcgataaa
     6841 atatctagat actttttttg tgagattctc tgttaattta atgcggtctc ctgcttttct
     6901 tttttcgaca tacagttgca catcagcagg aatccaaatg tcatgagaaa atagagacca
     6961 tgaatctgtt gctttgatgt cccctgcatt ttcaggtttg accagaccaa tccattctgt
     7021 ttcattcaaa tatattcctt gttctggaat cagcaacaag cgctggtctt ctgtctggct
     7081 agttgcttgt ttttcttttt tagagagtac aacaaaatga tactctcttt tcagtatcca
     7141 attattgcca atactgagtt gccattggct ttttttctgt tgccactggt ctaaaatcga
     7201 ctccatttgt tgttctttca aaataattcc tgtttttgag aatgctttgt cgaaaaagga
     7261 gtaaaaaaaa tagtatcgtt cagcatctgt catttcttca atcatttctg cagaaaatcg
     7321 aaattgttcc tcccactgtt cgacttcctt ttcgatcaac tgtcccatat atttttgaat
     7381 gacttggtct gcccattgga tctgttgcga aaattgctgg aaatgtctca acgcctgtgt
     7441 attttcttgt ttcaattgag gaactaccaa atgacgaagc cgatttctct ggatatctaa
     7501 caattggttt gtctgatcct caaagtaaac aagctgcgat tccgctgcat aagtatatag
     7561 gtcttctttg ctaaaactca gcaacggacg aatcagtctg cccgtagcaa atggttgtac
     7621 ttctttgatt ccagaaaaag tatttaactg tcccccacga acgattttca ttaacaccgt
     7681 ctcgatttga tcatctccgt gatgagcagt catcaacgtg tcatattgtc cttcttccat
     7741 cacgcgagtg aattcttgat aacgaaagtt acgagcttct gtttctatgg cggatttggc
     7801 tggatcttcc caaatactta catataaagg gagattcttc tcttcacaat atgagcgaag
     7861 ataggcagct tcttctgctg attcttctct taattggtga ttgatgtgga cgatacctaa
     7921 agaaaaaccg atttggcgtt gcacttgtaa aagtaaatgg agcaaaacca tcgaatcaac
     7981 accacctgat accgctgcta aaattctcga tccttgttgc cagaatcctt gttcattccc
     8041 gtgccgaata aatgactgct gtaatagcgg tgcttttttc ccgccttttt caacgttcat
     8101 cctttccaca ccctttcttc ctaaaaaaaa gagagcggga aaacgtctaa aacgacaatt
     8161 aacctgccct ctgttgatac tatgacccaa atagtcgcac tatcattatt tgcggctttt
     8221 tagctgcgac gtccgccacg gccaccgcgc tttccttctg tgtttcgctt caatgaagat
     8281 aagcggtcat cgctatcttt caaaaatgaa ctcatcaatg aatcgaagtc ttctttttga
     8341 gctgaaggtt gactgcgaga aaaagatttt ttctgtgctg gacgatttcg gttgtcacgg
     8401 ggatcttttt gttgatattt tttaggacgg ctttcttctc cagattcagc aggttgttcc
     8461 tgcgctttcc ggatagatag tccgattttt ccatcatcat ttactgaaat aactttaact
     8521 gttacttcat ctcctacact taaaacatca tggatgtctt ttacatagcc atttgacact
     8581 tcgctaatat gaactagacc cgtttttcca gcacctagat caataaacgc accaaaattt
     8641 gtgattcctg atacttttcc ttgcagtttt gctcctacct cgattgacat aaaaaaaatg
     8701 ttcctcctaa ttatttccct tttttatatt cctgcttatt ttgcagttgt agaactagtt
     8761 gattgatctg tttcatttgt cgagctgtct gtatttgatt ctaatatagg ataaactttt
     8821 tcaccgtctt ttgagtaaaa gaatttgctt ctggcaagtt tagctacata ctcatcatct
     8881 ttcagcaatg aaacatcttg gtgcagctga tcaacgtctg cgttgacttc tttcatttca
     8941 gtatttgctt cgactctcaa ttcgttcaat tgatgcatcc tttgcatgtc atggaaaatc
     9001 tgcacaccta ttatcgcgaa aatgaccaaa gcaccaacaa aaattgcagc cagtctgcgt
     9061 cttcgaaaaa tcagttgacg ttgttgttgc tgaaattcag catacttttt ctttgcatat
     9121 tccgtatcta gcgcagcgat tttattgctt tttttcgtca cttggattcg ctccaatctt
     9181 tttgattgtc tttttcatta taccaatatc gtgaaagctt gtctaatcta tttctctagt
     9241 gtttctgtgc gattttcaga aataatttcg tacatttttt gtgcatcttc ttttttggtt
     9301 gattcatgta attccgtaat tttcacttcc aacgttttgt tgccaaactg gactttcaaa
     9361 atatcgccta ctttgacatc actggaagat ttcgccaatt taccgttgat ttgaattctt
     9421 cctttatcag ctacttcttt tgctactgat cgtcttttga tgattcggga aatttttaaa
     9481 aatttatcta atcgcatatc ttattcctca ctttttaatt tttttattta atcgcaggat
     9541 tttcttacca aaaggcaaca ttagccattc tcgtactgta aacagacgaa gacgaatcac
     9601 tgcataaatg aacacagaag ccccgatccc aactcctatc acgctgacaa aaaaagcagt
     9661 ggaacgatga gttacaggac caaatactat attcaataca ccataataca gcagcagtac
     9721 tactgtcata caaagaaggc tgaacatcaa cttccgtcca aaatgccgtt ctttccaaaa
     9781 agcatttagc tcttttgttt caattcgaac aaaataccaa agcgtcacac ctaatcctaa
     9841 aagggtagac aggcttgcac ctgctgttcc taataaaccg gtcagaaaag aagtagtcaa
     9901 tccttttacc aatagtcccc atcctgcgcc ttttaaagaa gggcgaaacg agttcttgct
     9961 ttgagcaata ctttgataag cttggatgac tgcagtgaaa gcaatggaaa aaacaaataa
    10021 tacaagcgct gcatttcccg cataatcttt aaacaacgca taattgatat aagggagcag
    10081 caaggctaat cccaaagacg cagctaatgc taaagcggtc gtcaatctaa gatagatttt
    10141 ggctgtctgt aaaaactgac gatagatccg gttggtcaaa tgacgagtca acgcaggcag
    10201 gaatgtcgca cttaatgcag tggcaatcac taaacctaat tgtactaaag gctgacctcg
    10261 atcatagacc ccttttgcta tcttcgcacc gtattctgac agtccgtaaa cttccaaagc
    10321 attttttaca aagaaagagt cgatcagctg gaaaaagata agcaatccac tataaatcga
    10381 aaccagtccg ccttctacca gaaaacgccg gatcaagggc tttgacggct gtttgaacaa
    10441 tggaaaccgt cggaagctta acgctcctcc atgaatcttt tgttcatagt agtgaaggat
    10501 cccatatgcg cataaaccgc caaaaacagc tccactcatt gctgccgtcc ctgtttggta
    10561 caccgtccat ccaagctttc taaaagcaaa cgcactacag acaatcacaa aaacacgaac
    10621 gaactgctca actacttgag aaacagcagt tggttccatc aagaatcttc cttggaaatt
    10681 ccctcgataa aaagatagac caggcatcag caaaaaggta aaggaaacta cttggatcag
    10741 cggtttcaat tgtatgtctc ccatcatcca agcaatccag ctgctaaatg caaagacaaa
    10801 caaccacaaa aagacacctg tccaaaatac taagggataa agattttgca aagcttgctt
    10861 tcgctcgaaa ggattctttc tttctgcaac gtactttgat atgaactgcg gaagaccgga
    10921 taaagctaaa gtcattgcta tcccataaat tggataaact tgctgataaa cataaaaacc
    10981 ttcgtcgccg actaagtttt ggaggggaat tcggtaaagg gcacttaata cttttgctat
    11041 gaaagaagca atcgtcaata caaatgctcc ttgcatgacg cgccgcattt ctttatgatc
    11101 caaatcattg ctccttccta cgctgacaga tatttttgct cacgcaacgc tttgacaaac
    11161 ttttggattt cctgaagcca tactgcttct tccatttggt tggggatcgt caagcgtatc
    11221 accatctgtt ctttttcaac gcctagactg gcttttagtt ttgtcgctgt cagtgcttcg
    11281 aataactgtt ccacactata gcgttttgtt ccaactttac ttagagtaaa gactatatgc
    11341 gcctcctgtt tccttatcgt ttctaggagc gctcgatcgc catccatttt gatctgccca
    11401 attgtcaaga gatgtgctac ttcttctgga tattctccaa aacggtcgag cagatcatct
    11461 tctagttcat ctagcatttc ttgcgaatca agttcacgaa ttcgtttata gatctcgatt
    11521 ttctgccgtt gatctgccac gtatgtttca ggtagatacg catcgacacc gaggtcgatc
    11581 tcaacagtgg tcttctccac ttgactattc tttccttgtt tacgagaaac agcttcgctt
    11641 agcatttgag aatacatatc gaaaccgact gcatcaataa atccatgctg ctgagcacct
    11701 aagagattac ctgcaccgcg aatggaaaga tcccgcatcg caatcttgaa tccagatccc
    11761 agttctgtaa aatctttgat tgcctgaaga cgtttttcac tgacttcgtt aaggatcttc
    11821 tgctgctcat acataaagta agcatatgcg acacggctgc ttcttcctac ccgccctcgc
    11881 aattggtata acgtggataa tcccatataa tcagcgttct caacgaacaa cgtatttgcg
    11941 ttcgggatat ctactcctgt ttcaatgatg gttgtagtga caagaacatc atattgacct
    12001 tcaataaatt caaaaagcgt attttctaat tgcgcctctg tcatttgccc atgagcataa
    12061 ccgatccgtg cttccggaac gagttcttgg atttcttcta ctttttgttc gatcgtttct
    12121 acccgattat ataaatagaa tacttgtcca cctcgtccca tttctcgatg gattgcttcg
    12181 cgaatagccc caggattttt ttccataaca tacgtttgga tcggataacg attcgcaggc
    12241 ggtgtttcga ttactgaaag atcccgcacg cctaacatcg acatatgcaa agttctcgga
    12301 atcggtgttg cagtcagagt caaaacatcc acttgtgcac gtaactgttt caatcgttcc
    12361 ttatgtttta caccaaaacg ttgttcttcg tcaatgatca gtagacccaa atcgctaaat
    12421 tcaatatctt ttgacaaaat tcggtgggtt cctacaacaa tatcaacttg acctgtacgc
    12481 agttgttcga tggtttcttt ttgttgcttt cttgttctga aacgacttaa tagcccgata
    12541 tttacgggga aaccttcaaa tctttccagc atcgtttcat agtgctgctg tgccaagatc
    12601 gttgttggaa ctaaaaaggc tacttgtttg ctttccttta tcgctttaaa ggccgctcga
    12661 agcgctactt ctgttttccc ataaccaaca tcacctacga gtagtcgatc cattggtttt
    12721 tctttttcca tatcccgttt gatctccgct gcacttctca actggtcatc agtttcagag
    12781 taggggaagg cattttcgaa ttctttttgg taggcgtcat caggtccaaa agcatatcct
    12841 ttttctgact ctcttgcggc atataatttg attagatcgt cagcgatatc ctcgattttt
    12901 gaagagactt ttcgcttggt tttcgtccat tcacttccgc ctagcttatt gatgcgaggc
    12961 gctttggctt cagaggcgac atatttttgg atcagattca gttgagtaac aggaataaac
    13021 agtttgtcgt cgttttgata caaaatagtc atatagtctt ggtgaacgcc atctacttct
    13081 agcgtttcca tcccaatgta tttcccgata ccatgattgg catggacaac gtaatctcct
    13141 gcttttaact cattatagct tttcaatcgc tctgcgtttg ttactgtttg acgtctcgtt
    13201 tgttttttcg tcgttttttg gaaaatttct ttttctgtga tagctactat tttttcttga
    13261 ggaagttcaa aaccagcctg caaagctcct tcaactaatt gtacctgtcc tctgatcaat
    13321 gcattttttt ctgtttctac tactaatatg tcttcatctc gaagcatttg ttctgctttg
    13381 cgtattctgt cctttgtcgg aataaagatc acaacagtct gtttttgctt gcgccaacgg
    13441 tccatttctg ttttcagcaa tggcatttgt ccgaagaact gttgcatcgt ccgatattga
    13501 aaattatgga ttgcttggaa acgaagattt cccatccctt tttggaagag tgagaaaaag
    13561 gttgtggtga acttttcctt ctgcacgatt ttttgtatat ctgctgaaaa tgtctgctca
    13621 gaaaacaccc gcaattcgct gattttttga gtctgccatt ctgccgcttc cctttcgatt
    13681 tcccgatttg tttccatgat tcgctgataa tcatcaacga ctagtaaact atttgaaggg
    13741 aaataatcca agatcgtcac agattcttgg taaagaagat ctgagtagaa ttttgcttct
    13801 tctgtcggga cacctgcttg ccagcttgaa gtcagctgac cgaagtaatc ttgcaaaaac
    13861 tcgcggtctg cttcttctgt ggtcgcagca agccgttttt ctagcatttt ttgcatttgc
    13921 tctattccat gttctagatc ctctggtccg tagacttgtt cactcgttgg ggaaacccag
    13981 atttcttccg ttttttcgat tgagcgttgt gtttctgctt caaaataccg catagaatct
    14041 atctctacat caaatagttc tacacgaaca ggataggttg tcgttaacgg gtaaatatcg
    14101 ataatgcttc ctcgaatact gaattctccc ggttttgcga ccatctcttc tcgtacatag
    14161 cccatcaaag tcaattggcg aggaagattt tccacgtcta tctcatcacc aatatgccaa
    14221 ttcaattgcg cctgtagcca agttttctta ttcggcagtc gtttgtgcaa agatgcgact
    14281 ggcaacaaat agatacctgc ttttttttct tgaagtgcgt taagcgttgc aactctctcg
    14341 cctcgcgctt ctggagaaga aaaggccatt tcagcagaaa gcacttcatc cactggaaat
    14401 aaatggatct gatcttgcgg aatcacatgt tgcagatctt ctgctaactg attcgcataa
    14461 tacaaatttg gcatcagaat aatcatgttt ttatttgttt tcttaaacgc tcgagcaaat
    14521 agaagtgttt tcgctgagcc cgctaatcct gtgattagtt gtctaggcat tttttcttct
    14581 aaattgctga cccattcttt tacttgcgga tcttttccga ctaattgaat aatatccatt
    14641 ccttttcctc cttcctcaat tgaatcgatt catcgcatcc acgaacgaat gcccttctat
    14701 gagatattcg actgctttaa cagattgaca catgctttct tctatcaaag gctgattttc
    14761 tttgctgaaa ggactaagca catgctggac tactgttttt ttcccttctg gcctgccgat
    14821 tcccaccttg attcgatcaa agacattcgt attcaaatgg gaaataatac ttttgatacc
    14881 gttatgcccg ccggcacttc ccttttgacg gagccggatt tttcccactg ccagatccaa
    14941 atcatcgtag attacgacta attcctctgg ataaatccca aaataagtca ttagtgggcc
    15001 gactgcacga ccagattcat tcatgaatgt ttgtggtttg actaaaagga tcttttctcc
    15061 attatgaaaa aattcggcaa cttctgcttc gaacggattt tttttgaaag tagcttgatg
    15121 ctcgcgtgca attttatcca cgaccataaa gccgatattg tgttttgtat attggtattt
    15181 ggtacctggg tttcctaacc cgacgatcat tttcattttt gttttccacc taattctaaa
    15241 aagcagcaca aaaggctgga ctgctaatta gcggtccagt ccatcgagct tttttctttt
    15301 ttacacttgt caatgcatag tataacacaa aacaaggcgt ttcggctaaa agagaatcgt
    15361 taataaatga taaagaaatt aaaaatttct tcaaagttta atgaaataat tgatatataa
    15421 aaatagtgaa ataacgcaca tgtaagcttt tacatataat acaaccttgt tatctgttat
    15481 attttatagc caaatagcgt gacatggggc ttgagaaatg ttagcataag catgggaaaa
    15541 aacaaatcaa tggaaggatt gatactaaat gactgcagct gcagaaaaaa aagatcatca
    15601 aaaagttatt cttgttggag acggcgccgt aggttctagc tatgcctttg ctctagttac
    15661 tcaaaatatc gctcaagaag taggtattgt ggatatcaat actgctaaaa ctgaaggaga
    15721 cgcaattgac ttgtctcacg ctttggcatt tacttcacct aaaaaaatct attctgctac
    15781 ttacgcagac gcacatgatg ctgacttagt tgttattaca gctggcgcac cacaaaaacc
    15841 aggtgaaact cgtttagact tagtaaacaa aaacctaaaa atcaaccgcg atgttgtcac
    15901 acaaatcgtt gaatcaggat ttaacggtat ctttttagtt gccgctaacc cagtagatat
    15961 tttgacttac tcaacttgga aattctcagg tttccctaaa gaacgcgtaa tcggttcagg
    16021 tacctctctt gactcagctc gtttccgtca agcacttgcg gaattagttg acgttgatgc
    16081 acgtaacgtc catgcttaca tcttaggtga acacggagat tcagaattcc cagtttggtc
    16141 acatgcaaat gttgccggct tgcaaatcta tgaatgggta aaaaacaacc cagaaatcga
    16201 tgaagaagct atggttaacc tattctttgg cgtacgtgac gctgcttaca caattatcga
    16261 gaaaaaaggc gctactttct atggtatcgc agtagcatta gcacgtatca ctcgtgccat
    16321 cttagatgat gaaaatgctg ttcttccttt atcagtattc atgaatggcg aatatggatt
    16381 gaacgacatc tacattggtg cacctgctgt tatcaaccgt caaggtatcc aaaaagtaat
    16441 cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct gcttctgcta agcaattaaa
    16501 agaaattcta gatgaagctt ttgctaaact agacgctgaa taatctttga acatgacgga
    16561 aaacatctat tcggtgtttt ccgttttttt attttatcac ccttatggtt tctaaatttt
    16621 ttcataaagt agagataacc ttcgtctttt cctctaaatt atgctatcat gagaccgttc
    16681 caccctaaac ttttacttaa ggagttgtcg atatgacaag atcttcgcat cgaaaaaaat
    16741 cgaaacatgt tgctttgctt tcagttcttg gtgttctctt agtgctgatc agcttttata
    16801 cctatcgcag tacatactac acaaagcatt ttttgccgaa tacagaaata aatggaataa
    16861 atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt aaaagaagct tattctgata
    16921 aaaaactggc tattaaagaa gatggaaaag tctggcagga agtaccaaaa tccgagctcg
    16981 gctacaaaga tgatttcact tcggagcttt ctccgatcct aaatgaacaa aacggatgga
    17041 cttggggaat gacttatgtc tcagcggcag aaaaacaaga aatagatcct gtcagtcaaa
    17101 accaccaaaa actagacact accgttcaga cattgacaac taagctgact gatttgaaca
    17161 aagaccggac acctactact gatgcaacga tcgaaaaagc aggtgactct ttcgttatca
    17221 agcctgaagt caatggaaat acgatcgacg tcgatgctgc ggtcaaacaa ttaaaaagtg
    17281 cggtaaactc tggtaaagat acaattgaat tgactgaatt caaagaaaaa ccaaaagtaa
    17341 catctgaaga cagctcactg aaagaacaac tcgcatccat gaatgctatc gcaaatgtaa
    17401 aagctactta cagtataaac ggtgagactt tccagatccc atcaagtgat attatgagtt
    17461 ggctgaccta caatgatgga aaagtcgatt tagatacaga acaagtacgt caatatgtaa
    17521 cagatttagg cacaaagtat aacacaagta cgaatgacac aaaatttaaa agtacaaaaa
    17581 gaggagaagt tacggtccca gttggcactt acagttggac gatccaaaca gacagtgaga
    17641 cagaagcatt gaaaaaagcg attctggcag gccaagattt tactcgttca cccattgtgc
    17701 aaggtggcac aaccgctgat catccattga tcgaagatac gtatatcgaa gttgatctgg
    17761 agaaccagca tatgtggtat tacaaagacg gaaaagtggc ccttgaaaca gatatcgttt
    17821 ccggaaagcc aactacacct acacctgctg gagtcttcta tgtttggaat aaagaagaag
    17881 atgcaacttt aaaaggaaca aatgacgatg gaacacctta cgagagtccg gtaaactact
    17941 ggatgccgat tgactggaca ggtgttggta tccacgattc agactggcaa cctgaatacg
    18001 gtggagactt atggaaaacc cgcggttcac acggctgcat caacacccca ccaagtgtaa
    18061 tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt attggtcttc taacaaaaat
    18121 aatatataaa aagaaagcac ccgtacgatt ggtacaggtg ctttcttttt attatctaac
    18181 aatgtgctac ttacatttca gcagcagtaa agacttctga aacatcatca tcgtcttcta
    18241 gcttatctac tagacgttcc aattgctctt tttgttcctc atttaaatca atcgtcgttt
    18301 gtggaatcat cgtcaattct gcttgcgcca aagtgaactc tttttccaac tcatctcgta
    18361 cagcagcaaa atcttctggt gcagtataaa tctcgaatac ttcaggagaa gtttgcagat
    18421 cttctgcccc cgcctctaat acatcctcaa acatcgtctc ttcatcaaca gtcaacccat
    18481 cgcgttcaat agcgatatat cctttgcggt caaacaagta actgactgaa cccgtttctc
    18541 ccagtgatcc accgtttcga gtaaaagcta ctcggacatt tgtcgctgtc cgattacggt
    18601 tatctgtcaa tgcatggact aagattccta ctccacccgg accatatcct tcatacgtga
    18661 tttcatcgta attcgtttca tctacggcac tcgtagcctt tttaatcgcc cgttccacat
    18721 tgtcgttagg catatttgca gcttttgctt tgtccacaac aagccgcaaa gctggattca
    18781 tagatggatc aggaccgcct gctttagctg ccatataaat ttcacgagaa agtttctgaa
    18841 agactttccc tcgttttgca tcttgggcat tttttcttcc ttggatatta tgccatttac
    18901 tatgtcctga catgattttt cctcccttgt tcttttgaga atacacttct attctagtac
    18961 tatttcacaa aaaaacaaag ccacagccaa tctataaacg cttcttgaat gccgcaaaaa
    19021 gcacacataa tatcacagca gtcagagcac cgatcgaatc caacatgaca tcttggaaca
    19081 taggtgttcg tccaccggtc atcatctgat ggaattcgtc caaacctgcg taccctgttg
    19141 ctgaaagcca agcaaggatc gctgtcagcc accaaatttt tagcttgggg aaaacaccta
    19201 aaaacaagct gcctcccaaa ataaaatagg taccgaaatg tgcgcccttc cttaaaaaga
    19261 actctaaaaa tttggcgtag cccagatggt tgatactcac catacttcca ccataattaa
    19321 aacgtatacc cgctaatgtg tctttaaatg gctgattagg cagccagctt tctaatcgcg
    19381 aaacttgtga ttgctgtccg taagtttgag aagaactgta aaataagatt ataacgacta
    19441 tcagtgcgat tactaaataa atatttccgt ttttcatttg tttttttgaa gaattcataa
    19501 gtcacctctg tattttatct tagacttttt ttgaagaaac aacaatcatt ttcatagaga
    19561 cttcttccat aaaaaagtgc tatacttaat tttgtattac aatatgaagg gagttttttc
    19621 atgagttatt cattaaattg gaatctagat tctatttttt ccggaggcag tcattctgat
    19681 gctttgaacc aacggatgaa gcaattggaa gaccaaatga acgagtatta tcaccgcgta
    19741 acaaaatggt cacctagttc tgacaatgca gagcagttaa acgctattct tcaattgcaa
    19801 gaaacgatta cgaatggttt tacccaatgc agcagctata ttactgcatt gctttctgcc
    19861 aatgtcaatg attcagatgc aaaggtctta tcaggcaagc tttacgcaat gctgcctcgt
    19921 ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag aaatatctga caatgattgg
    19981 aatcagatgc tttctcaatc ttcgtttgaa acaattgctt ttcgtttaaa cgagattcgt
    20041 cgtgatggct cacaattatt atcagaagct gaagaaaaca ttataaatac gctttcttta
    20101 gatggtctaa atgcttggag cagtcattat gatacgattg tagccagcat ttcgattccc
    20161 ttcgaacaag atggacaagt agtcgaatta tctgctggac aagccttcaa taagatgatg
    20221 ggcgatccag atccaaaagt cagagaaaca cttttcgcag catgggaaga agcctggaaa
    20281 gaaaaggcat ccctcttcac ggatactttg aatcatttag atggttttag actttctgac
    20341 tatgaacttc atggtgtcac agattttcta caaaaaccat tagagtacaa tcggttgaag
    20401 aaagaaacat tgtctgtcat gtgggataca atccagaaaa acaaacaacc ccttgttaat
    20461 tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa tggaatggca agatcaagat
    20521 gcacctatta ttttaggcga tttgaaagaa aaaaccttta gttttgacga agcagcagca
    20581 tttattatcg aaaactttaa caaattcagt ccaaaaatgg ctaaatttgc ccaatctgct
    20641 tttgaaaaga gctggatcga agcagaagat cgtcctggga aacgtcctgg tggttattgt
    20701 actgagttac cagaaacaaa agaatcaagg atctttatga cctacagtaa ttctgttaat
    20761 gaggttgcga cacttgctca tgaactagga catgctttcc atagcagcac gatgtgggat
    20821 ttaccttctt taaatcgcga atatgccatg aatgtcgcag aaacagcaag tacattcgca
    20881 gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa caaaagaaga aaaaatcaat
    20941 ttgctagata caaaactgca aaatgcttta gctatgttca tgaatatcca ttcccgattc
    21001 atttttgaaa atcgattcta tgaagcacgg caagaaggat tagtctcaga agataagatc
    21061 acagagatga tggttgaggc gcaaaaagaa ggctatcaag gagctttagc tacttaccat
    21121 ccatatttct gggctgcaaa attgcatttc tttattgatg acgtaccgtt ttataacttc
    21181 ccatacacat ttggctacct ctttagtttg ggcatctatg cttacgccaa ccaaaaggga
    21241 gcaaattttg aagaagaata tatccattta ctacgcgata ctgcctcaat gacgacggaa
    21301 gatcttgcga agaaacattt gaatgtcgat cttacccaac cagatttttg gcaagcaggt
    21361 atcgacatgg tgactgaaga tatccaaacg ttcatggaat tgacagaaga atttatctga
    21421 tttctatttt atcacaataa aaaaagagtt agtccagaat gggctaactc tttttgatta
    21481 atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa ccaactttta
    21541 cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca atcgctacgt
    21601 ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct tgagaaactg
    21661 aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca cgaccagtta
    21721 cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga tctttaccgc
    21781 cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct gcaccattca
    21841 cacttccaga aggagctgga gcaggtgtta caggttttgg ttgttcaggt gttggtgttg
    21901 gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt tgatctgttg
    21961 atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca ggcactgttg
    22021 ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt
    22081 caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat tcttctgttg
    22141 aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat gatgattctt
    22201 cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt gcagtagatg
    22261 aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt tcagcttttt
    22321 cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga atacgagctt
    22381 gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct tcagcagttg
    22441 cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta gataaaagat
    22501 cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt ttagcatcgt
    22561 tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc atcaagtcgt
    22621 tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct gctaatgaat
    22681 cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg
    22741 cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct gcaatgtctt
    22801 ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt
    22861 gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt gcatctgctt
    22921 gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa tcaaaatcgt
    22981 ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat accattactg
    23041 ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta ataaatatct
    23101 aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg tgtcaacgat
    23161 ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa aactgttcgt
    23221 ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga taacaacgat
    23281 tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga ataatttctt
    23341 aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag gtcctaaaaa
    23401 ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact ggaccaccat
    23461 cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag taaagatgtt
    23521 gacatggata acgtctttca ttacatacat caaccataca attaaaggga gagctactgc
    23581 atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa caatcgtggt
    23641 aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca atatcagaaa
    23701 gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg ttaaatgacc
    23761 atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg tttctttctt
    23821 caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca cgtcatacat
    23881 ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg gtttcgtttt
    23941 aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg atgtctggac
    24001 aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta aggcatggag
    24061 cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac
    24121 tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag acgaagccgt
    24181 attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca tatttgcttc
    24241 gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt caggcgaacg
    24301 agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca actcaagttc
    24361 ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa cgtcatcata
    24421 gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa gattatggac
    24481 agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat caaccattgc
    24541 aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg ctcgctgcgc
    24601 agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa taataatatt
    24661 tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc ttgtgttcaa
    24721 ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct tttttttagc
    24781 aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg tttatggatc
    24841 aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct gcggtatctg
    24901 aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt tccaatacat
    24961 acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa gctaaatact
    25021 tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac aaataatttt
    25081 gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc atacctaacc
    25141 agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg
    25201 cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta tcgtcttttt
    25261 ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc tcttcgttcg
    25321 acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga agcaggtaca
    25381 gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac tgcggccagt
    25441 tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc tcttttaatc
    25501 gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca acgaagacat
    25561 ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca tctacttcga
    25621 ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt ttttctactg
    25681 tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact agtacttgat
    25741 cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg ataactactc
    25801 cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga ggagttgctt
    25861 cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg tagttttctg
    25921 gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg attgcctgaa
    25981 caatatttgg ccctaatgtg atatttggga caaattggcc atccattaca tctacgtgga
    26041 tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg gcaaaatctg
    26101 cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt ttaccttctt
    26161 tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa taattgtcgt
    26221 aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag ccaggttctt
    26281 tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat tcaggaaatt
    26341 gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat cctggtgtat
    26401 ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta tgccgtcctc
    26461 ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat atttgattca
    26521 gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt tctgggaagt
    26581 aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga tagcctattt
    26641 tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag tcggtctttg
    26701 tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag cggtcaagta
    26761 gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga tcaacatttg
    26821 tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga tagccgtctg
    26881 tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt ttccgattcc
    26941 ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca tagtaaaaac
    27001 cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt gtttatttta
    27061 ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat atggcttgaa
    27121 aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat gtcgcattcc
    27181 cttctgatat attttattca gcacttattt tgaactttct gaggtgctag ctgatgaact
    27241 tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc tacttgagct
    27301 gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg gtcctttaga
    27361 gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg gtactgtacg
    27421 aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc cgatatattc
    27481 tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg agatatcatt
    27541 caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt ctgctacaac
    27601 ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt cgctatagtc
    27661 ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat tacgaacagt
    27721 ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt tgctgactgt
    27781 gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag attgcgaaat
    27841 gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca agtcagcaga
    27901 aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac tgtaatcatc
    27961 taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt tttgcttgac
    28021 tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt tgtcatctgc
    28081 aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt ctctagcaga
    28141 agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc cagaagcaaa
    28201 gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca gccaccattt
    28261 tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta aggtttcgtt
    28321 ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg gtgcatcttc
    28381 cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc atttttcttc
    28441 gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct cggctgactt
    28501 ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg
    28561 cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat
    28621 tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat aaaggatgat
    28681 ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct gctcagggga
    28741 cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc
    28801 gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca gtacgttttg
    28861 gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag cagaaaggat
    28921 ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt ggatatatcg
    28981 tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt cttcttcacc
    29041 aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta aagcttctct
    29101 ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc
    29161 tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac caccgctacc
    29221 gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga tcatacactg
    29281 tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc ttcattggca
    29341 cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat gatcgttcct
    29401 atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag tcgatctttt
    29461 agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc gacagaacgt
    29521 gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc tcctgatttg
    29581 actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc ttcacgaatc
    29641 aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga cagagcggcc
    29701 gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg ctgataaatc
    29761 agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc atcgtcaaca
    29821 gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct
    29881 ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc tgaacggctg
    29941 gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat
    30001 tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa aaatccatct
    30061 gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga ttgtagcata
    30121 ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca
    30181 acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat accggaagac
    30241 tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa attcgctaat
    30301 tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc aagacctgaa
    30361 caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt actgacttct
    30421 ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc tgcattatct
    30481 tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc accgcatgac
    30541 ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag
    30601 acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg
    30661 atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt tttcgctact
    30721 ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg caaaacagtg
    30781 atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg gctaggctga
    30841 tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac cagtcgtttc
    30901 gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc agcaatgcct
    30961 tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac aaatttccct
    31021 gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca
    31081 gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat cttgacccag
    31141 tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga
    31201 tggctaatcg taccatagat caattctgta aataaaccca catctttttg atttagctcg
    31261 ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt gttcactcgc
    31321 tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg gatttttttc
    31381 attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg aaatcatgta
    31441 cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg gtctgtttac
    31501 cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt gctttgtctg
    31561 tagtttctgc caatacttca accgataata atttccagcg tatgccctca taggtcgtga
    31621 atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct gcagttttat
    31681 gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct tctgactcgt
    31741 cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc aatagctttc
    31801 ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta gtgattggta
    31861 tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc atgatcgtta
    31921 caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct ccccgatatt
    31981 taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc aataactttt
    32041 caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat tcaatgattt
    32101 cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata ccgtgcttca
    32161 gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg
    32221 gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca agaatcggca
    32281 cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc ctccatatat
    32341 tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa gataccgtct
    32401 aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt cacttctatc
    32461 tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt gaccgttcca
    32521 tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt tgcttcgatg
    32581 atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac agcaattctc
    32641 ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat cattgtctca
    32701 tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg ctgagcagtt
    32761 ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg gccctttctt
    32821 aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc tgttctttct
    32881 ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt ttatatttga
    32941 tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc gttggcccta
    33001 atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga aagattttct
    33061 ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag taataaggag
    33121 gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca taattttgtt
    33181 ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg acttcgcctg
    33241 ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat gtatgctcac
    33301 tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca actaatgtca
    33361 cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata tctgcttctt
    33421 ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg gtatctacat
    33481 ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct actttttgag
    33541 tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga ttgggaatag
    33601 gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc agtgtaagag
    33661 agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc acaaaagacg
    33721 aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat cgattttgta
    33781 ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac tcttcacgca
    33841 tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg tttaactgca
    33901 ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc
    33961 ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct ctcgcatgat
    34021 aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata atgatcagcc
    34081 cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact tgcgcttctt
    34141 cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga
    34201 ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga gtcaatgcga
    34261 tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg attgcctgaa
    34321 gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc tgatcttttt
    34381 gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca ttcagttcca
    34441 atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt tctgattcta
    34501 taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg attcctttat
    34561 ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa agaagttctt
    34621 tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc ttttcagtta
    34681 acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact tcatatttga
    34741 tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct tcttcttctg
    34801 cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct ggtgtgtctg
    34861 ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc atcgtttgta
    34921 gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat gaaagtaatt
    34981 cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa gatggattag
    35041 ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga ttgccattac
    35101 caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca gagggaacca
    35161 aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact tcagctgtta
    35221 atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa aaagttcctt
    35281 gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct aacaaaaaat
    35341 taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc cttttctacc
    35401 agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc tttttagatg
    35461 ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat tccaattcgt
    35521 gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt
    35581 ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct aaagcttgac
    35641 cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca tccaattcat
    35701 gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga tctaatagtg
    35761 agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt aatatattta
    35821 ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat tcgttcaact
    35881 gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat ttcgatttct
    35941 tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc tcggcctacg
    36001 atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac accatcgggc
    36061 actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac atcttttcct
    36121 tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc gacatattct
    36181 gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt gcttcggaag
    36241 aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat tgagatcgaa
    36301 tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc ttttcctaca
    36361 ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac gtcctttcaa
    36421 ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt caaggttatc
    36481 tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc
    36541 tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt ttttcgtttg
    36601 gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa
    36661 gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac atttttgcat
    36721 aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag
    36781 attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg
    36841 aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg ctaaaagccc
    36901 atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct
    36961 gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg
    37021 taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct
    37081 ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca
    37141 cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa
    37201 tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt
    37261 taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga
    37321 tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca tctagttttt
    37381 gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg
    37441 ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt
    37501 tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta
    37561 gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt
    37621 tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg
    37681 ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagca
    37741 cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa
    37801 ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt
    37861 tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact
    37921 gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta
    37981 tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt
    38041 ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct
    38101 cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg
    38161 cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga
    38221 ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata
    38281 gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat
    38341 aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac
    38401 gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt
    38461 gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt
    38521 gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca
    38581 tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca
    38641 tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc
    38701 aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca
    38761 aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat
    38821 agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc
    38881 tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga
    38941 taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa
    39001 atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac
    39061 cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag
    39121 aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt
    39181 ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga
    39241 gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc
    39301 cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt
    39361 cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata
    39421 aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg
    39481 aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa
    39541 tctgataagc accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct
    39601 ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt
    39661 ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag
    39721 cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat
    39781 taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata
    39841 ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat
    39901 gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt
    39961 tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca
    40021 gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa
    40081 ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag
    40141 agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt
    40201 tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg
    40261 actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg
    40321 actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat
    40381 ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca
    40441 gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag
    40501 tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa
    40561 ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag
    40621 aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta
    40681 gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat
    40741 gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg
    40801 aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg
    40861 atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa
    40921 ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga
    40981 cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc
    41041 ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc
    41101 ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt
    41161 tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc
    41221 attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc
    41281 actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg
    41341 atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc
    41401 atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg
    41461 acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc
    41521 agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat
    41581 gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct
    41641 gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg
    41701 aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca
    41761 caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc
    41821 agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg
    41881 gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac
    41941 ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa
    42001 ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat
    42061 gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg
    42121 tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt
    42181 gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg
    42241 caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg
    42301 gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa
    42361 gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat
    42421 gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt
    42481 tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag
    42541 gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca
    42601 actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc
    42661 cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg
    42721 attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat
    42781 ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt
    42841 tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc
    42901 aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat
    42961 tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg
    43021 acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag
    43081 aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga
    43141 cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca ccaacataag
    43201 tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc
    43261 cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat
    43321 ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct
    43381 caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg
    43441 tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg
    43501 catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt
    43561 taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca
    43621 ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga
    43681 tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta
    43741 acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa
    43801 gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt
    43861 cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac
    43921 ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg
    43981 gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt
    44041 gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc
    44101 ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt
    44161 tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta
    44221 aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat
    44281 aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta
    44341 tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa
    44401 aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc
    44461 ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg
    44521 acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc
    44581 ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta
    44641 atactcagga caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt
    44701 tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct
    44761 tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata atttcctggc
    44821 acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa
    44881 tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt
    44941 tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg
    45001 aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc
    45061 ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt
    45121 atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg
    45181 gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat
    45241 ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag
    45301 agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat
    45361 tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca
    45421 gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt
    45481 cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag
    45541 gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg
    45601 cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta cattatataa
    45661 gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt
    45721 aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat
    45781 ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa
    45841 caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct
    45901 agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt
    45961 gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa
    46021 aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat
    46081 catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag
    46141 aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc
    46201 taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat
    46261 acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc
    46321 agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc
    46381 caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa
    46441 aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga
    46501 ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc
    46561 ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt
    46621 ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc
    46681 attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt
    46741 ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta
    46801 gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc
    46861 cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct
    46921 ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg
    46981 atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata
    47041 cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc
    47101 agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa
    47161 atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca
    47221 tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac
    47281 ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc
    47341 gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac
    47401 tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat
    47461 cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg
    47521 ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa
    47581 ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc
    47641 ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa
    47701 agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt
    47761 tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc
    47821 ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc
    47881 tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa
    47941 ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt
    48001 tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc
    48061 ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt
    48121 caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc
    48181 acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa
    48241 caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt
    48301 caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg
    48361 gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt
    48421 taggattcct cagtttttca tcacctattt tacttcttat attttattgc agggttcgta
    48481 ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat
    48541 aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc
    48601 cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat
    48661 cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt
    48721 cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg
    48781 tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag
    48841 gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt
    48901 aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca
    48961 gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta
    49021 atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt
    49081 atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa
    49141 atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat
    49201 ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat
    49261 ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct
    49321 cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc
    49381 ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct
    49441 tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac
    49501 tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga
    49561 aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt
    49621 tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca
    49681 tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc
    49741 ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat
    49801 agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct
    49861 tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac
    49921 cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc
    49981 gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca
    50041 gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct
    50101 gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg
    50161 tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg
    50221 caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca
    50281 ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac
    50341 caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc
    50401 gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt
    50461 tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta
    50521 aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa
    50581 acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg
    50641 caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc
    50701 aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa
    50761 tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac
    50821 caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc
    50881 tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga
    50941 atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg
    51001 atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa
    51061 ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat
    51121 gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat
    51181 ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt cctaagatca
    51241 tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag
    51301 caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga
    51361 tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa
    51421 gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga
    51481 aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac
    51541 tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag
    51601 tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct
    51661 ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt
    51721 tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc
    51781 cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca
    51841 gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc
    51901 aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta
    51961 acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca
    52021 tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac
    52081 cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg
    52141 atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc
    52201 ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg
    52261 aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa
    52321 actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt
    52381 ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag
    52441 aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga aacagcgctt
    52501 gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca
    52561 ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg
    52621 atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc
    52681 attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg
    52741 actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt
    52801 atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa
    52861 gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc
    52921 atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat
    52981 aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt
    53041 gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg
    53101 aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat
    53161 atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg
    53221 aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat
    53281 attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg
    53341 tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc
    53401 cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc
    53461 agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc
    53521 aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc
    53581 taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact
    53641 agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc
    53701 gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga
    53761 tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc
    53821 caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc
    53881 cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg
    53941 accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac
    54001 agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt
    54061 cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa
    54121 ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga
    54181 taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt
    54241 ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga
    54301 gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa
    54361 acgattctct tgagatttta attctaactt actttctctc agctcacttt caacttcaga
    54421 acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc
    54481 ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt
    54541 ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat
    54601 agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc
    54661 atctatacag cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat
    54721 gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa
    54781 ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca
    54841 ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta
    54901 gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct
    54961 ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt
    55021 gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct
    55081 gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc
    55141 atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga
    55201 tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg
    55261 tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt
    55321 ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata
    55381 tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat
    55441 ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca
    55501 cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta
    55561 cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg
    55621 tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc
    55681 ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa
    55741 agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc
    55801 tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt
    55861 gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg
    55921 taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt
    55981 tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt
    56041 tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt
    56101 atatttacaa atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa
    56161 gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa
    56221 agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga
    56281 tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat
    56341 tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt
    56401 gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat
    56461 tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta
    56521 acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag
    56581 gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc
    56641 actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt
    56701 tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt
    56761 ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg
    56821 atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa
    56881 aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga
    56941 cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac
    57001 gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga
    57061 accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt
    57121 gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg
    57181 aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat
    57241 aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga
    57301 ttatctccta caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac
    57361 aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt
    57421 atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt
    57481 ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa
    57541 taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa
    57601 agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc
    57661 cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca
    57721 cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac
    57781 cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag
    57841 ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta
    57901 aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg
    57961 cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg
    58021 ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta
    58081 accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca
    58141 gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga
    58201 aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga
    58261 ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt
    58321 tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa
    58381 gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat
    58441 gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac attttttgga
    58501 ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca
    58561 ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc
    58621 tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct
    58681 aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata
    58741 tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca
    58801 cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag
    58861 gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt
    58921 ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca
    58981 accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc
    59041 tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat
    59101 cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca
    59161 gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa
    59221 tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca
    59281 tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa
    59341 gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa
    59401 agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa
    59461 cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct
    59521 gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt
    59581 aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag
    59641 atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
    59701 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta
    59761 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    59821 cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat
    59881 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    59941 cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat
    60001 ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt
    60061 ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg
    60121 gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca
    60181 gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt
    60241 cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat
    60301 tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc
    60361 ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg
    60421 tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca
    60481 aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct
    60541 aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct
    60601 tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct
    60661 tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct
    60721 tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca
    60781 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
    60841 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
    60901 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
    60961 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
    61021 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
    61081 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
    61141 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
    61201 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
    61261 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
    61321 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
    61381 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
    61441 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
    61501 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
    61561 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
    61621 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
    61681 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
    61741 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
    61801 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
    61861 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
    61921 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
    61981 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
    62041 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
    62101 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
    62161 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
    62221 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
    62281 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
    62341 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
    62401 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
    62461 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat aattcaaact
    62521 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
    62581 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
    62641 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
    62701 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
    62761 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
    62821 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
    62881 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
    62941 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
    63001 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
    63061 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
    63121 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
    63181 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
    63241 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
    63301 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
    63361 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
    63421 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
    63481 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
    63541 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
    63601 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
    63661 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
    63721 cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt catcaacttc
    63781 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
    63841 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
    63901 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
    63961 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
    64021 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
    64081 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
    64141 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
    64201 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
    64261 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
    64321 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
    64381 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
    64441 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
    64501 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt
    64561 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
    64621 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
    64681 atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg
    64741 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
    64801 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
    64861 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
    64921 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
    64981 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
    65041 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
    65101 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
    65161 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
    65221 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
    65281 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
    65341 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
    65401 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
    65461 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
    65521 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
    65581 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
    65641 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
    65701 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
    65761 tcctgtttct ttgagctgtt gtacagcttg taccaaattc gtcacgcgtg caacagggac
    65821 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
    65881 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
    65941 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
    66001 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
    66061 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
    66121 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
    66181 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
    66241 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
    66301 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
    66361 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
    66421 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
    66481 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
    66541 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
    66601 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
    66661 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
    66721 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
    66781 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
    66841 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
    66901 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
    66961 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
    67021 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
    67081 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
    67141 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
    67201 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
    67261 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
    67321 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
    67381 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
    67441 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
    67501 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
    67561 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
    67621 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
    67681 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
    67741 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
    67801 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
    67861 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
    67921 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
    67981 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
    68041 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
    68101 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
    68161 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
    68221 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
    68281 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
    68341 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
    68401 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
    68461 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
    68521 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
    68581 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
    68641 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
    68701 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
    68761 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
    68821 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
    68881 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
    68941 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
    69001 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
    69061 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
    69121 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
    69181 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
    69241 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
    69301 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
    69361 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
    69421 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
    69481 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
    69541 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
    69601 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
    69661 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
    69721 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
    69781 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
    69841 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
    69901 gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg
    69961 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
    70021 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
    70081 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
    70141 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
    70201 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
    70261 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
    70321 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
    70381 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
    70441 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
    70501 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
    70561 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
    70621 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
    70681 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
    70741 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
    70801 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
    70861 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
    70921 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
    70981 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
    71041 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
    71101 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
    71161 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
    71221 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
    71281 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
    71341 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
    71401 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
    71461 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
    71521 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
    71581 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
    71641 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
    71701 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
    71761 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
    71821 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
    71881 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
    71941 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
    72001 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
    72061 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
    72121 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
    72181 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
    72241 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
    72301 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
    72361 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
    72421 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
    72481 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
    72541 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
    72601 aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg
    72661 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
    72721 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
    72781 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
    72841 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
    72901 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
    72961 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
    73021 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
    73081 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
    73141 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
    73201 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
    73261 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
    73321 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
    73381 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
    73441 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
    73501 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
    73561 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
    73621 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
    73681 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
    73741 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
    73801 acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac
    73861 atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa
    73921 gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt
    73981 tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga
    74041 ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga
    74101 acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct
    74161 tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct
    74221 gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag
    74281 caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt
    74341 gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa
    74401 agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc
    74461 gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat
    74521 aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt
    74581 tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat
    74641 cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca
    74701 tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac
    74761 agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga
    74821 aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac
    74881 aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc
    74941 tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc
    75001 ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc
    75061 agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag
    75121 acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca
    75181 aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga
    75241 cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag
    75301 aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg
    75361 gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt
    75421 tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc
    75481 aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg
    75541 tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag
    75601 cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac
    75661 cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg
    75721 atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata
    75781 ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg
    75841 cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg
    75901 ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc
    75961 gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt
    76021 tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg
    76081 gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt
    76141 tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt
    76201 tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc
    76261 ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt
    76321 ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat
    76381 tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga
    76441 ttacgctact gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc
    76501 acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc
    76561 ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt
    76621 ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa
    76681 gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt
    76741 tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct
    76801 gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta
    76861 ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta
    76921 attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca
    76981 ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg
    77041 agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt
    77101 tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt
    77161 tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc
    77221 gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt
    77281 tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg
    77341 tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    77401 ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg
    77461 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
    77521 tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc
    77581 ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat
    77641 aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac
    77701 acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga
    77761 agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat
    77821 aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt
    77881 tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg
    77941 ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag
    78001 cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa
    78061 tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac
    78121 tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt
    78181 tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa
    78241 tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg
    78301 gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt
    78361 accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac
    78421 accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc
    78481 agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact
    78541 agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa
    78601 cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc
    78661 tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact
    78721 tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc
    78781 catccctttc tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt
    78841 aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt aggattttta
    78901 tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg
    78961 aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc
    79021 ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa
    79081 aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg
    79141 aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc
    79201 tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca
    79261 tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac
    79321 gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc
    79381 aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa
    79441 tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc
    79501 gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca
    79561 agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca
    79621 ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac
    79681 ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca
    79741 ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc
    79801 atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta
    79861 aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg
    79921 aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa
    79981 ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct
    80041 tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta
    80101 gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac
    80161 ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg
    80221 atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg
    80281 ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt
    80341 cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa
    80401 gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg
    80461 aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac
    80521 tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt
    80581 acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc
    80641 atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata
    80701 gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg
    80761 cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat
    80821 ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg
    80881 ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat
    80941 cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt
    81001 tttctccaat tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc
    81061 tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact
    81121 aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt
    81181 tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag
    81241 acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc
    81301 cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata
    81361 ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa
    81421 aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat
    81481 ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc
    81541 aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct
    81601 atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa
    81661 aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct
    81721 gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa
    81781 ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa
    81841 tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca
    81901 aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt
    81961 tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata
    82021 ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg
    82081 aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata
    82141 gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg
    82201 taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat
    82261 tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc
    82321 atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag
    82381 atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa
    82441 gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat
    82501 gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt
    82561 tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa
    82621 tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac
    82681 ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg ctttttctgt
    82741 tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt
    82801 tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg
    82861 caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt
    82921 aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat
    82981 ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga
    83041 ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcag
//
LOCUS       DANKHK010000002        68227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000002 DANKHK010000000
VERSION     DANKHK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            27..908
                     /locus_tag="PW109_000077"
     CDS             27..908
                     /locus_tag="PW109_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2509963.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            921..2531
                     /locus_tag="PW109_000078"
     CDS             921..2531
                     /locus_tag="PW109_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2509964.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWKPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2608..3267)
                     /locus_tag="PW109_000079"
     CDS             complement(2608..3267)
                     /locus_tag="PW109_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2509965.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3550..4215)
                     /locus_tag="PW109_000080"
     CDS             complement(3550..4215)
                     /locus_tag="PW109_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2509966.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4362..4967)
                     /gene="dhaL"
                     /locus_tag="PW109_000081"
     CDS             complement(4362..4967)
                     /gene="dhaL"
                     /locus_tag="PW109_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2509967.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4995..6005)
                     /gene="dhaK"
                     /locus_tag="PW109_000082"
     CDS             complement(4995..6005)
                     /gene="dhaK"
                     /locus_tag="PW109_000082"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2509968.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5980..6426)
                     /gene="dhaM"
                     /locus_tag="PW109_000083"
     CDS             complement(5980..6426)
                     /gene="dhaM"
                     /locus_tag="PW109_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2509969.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6529..6849
                     /locus_tag="PW109_000084"
     CDS             6529..6849
                     /locus_tag="PW109_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2509970.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6961..7830)
                     /locus_tag="PW109_000085"
     CDS             complement(6961..7830)
                     /locus_tag="PW109_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2509971.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7926..9230)
                     /locus_tag="PW109_000086"
     CDS             complement(7926..9230)
                     /locus_tag="PW109_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2509972.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVRLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9227..9736)
                     /locus_tag="PW109_000087"
     CDS             complement(9227..9736)
                     /locus_tag="PW109_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509973.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9911..12046)
                     /locus_tag="PW109_000088"
     CDS             complement(9911..12046)
                     /locus_tag="PW109_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2509974.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12129..13784)
                     /locus_tag="PW109_000089"
     CDS             complement(12129..13784)
                     /locus_tag="PW109_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2509975.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13786..14100)
                     /locus_tag="PW109_000090"
     CDS             complement(13786..14100)
                     /locus_tag="PW109_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2509976.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRAPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14270..14530
                     /locus_tag="PW109_000091"
     CDS             14270..14530
                     /locus_tag="PW109_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2509977.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14544..14858
                     /locus_tag="PW109_000092"
     CDS             14544..14858
                     /locus_tag="PW109_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2509978.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15588..16142)
                     /locus_tag="PW109_000093"
     CDS             complement(15588..16142)
                     /locus_tag="PW109_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2509979.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16268..18197)
                     /locus_tag="PW109_000094"
                     /pseudo
     CDS             complement(16268..18197)
                     /locus_tag="PW109_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18503..19219)
                     /locus_tag="PW109_000095"
     CDS             complement(18503..19219)
                     /locus_tag="PW109_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2509980.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19220..21043)
                     /gene="glpO"
                     /locus_tag="PW109_000096"
     CDS             complement(19220..21043)
                     /gene="glpO"
                     /locus_tag="PW109_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2509981.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21057..22553)
                     /gene="glpK"
                     /locus_tag="PW109_000097"
     CDS             complement(21057..22553)
                     /gene="glpK"
                     /locus_tag="PW109_000097"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2509982.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22689..24149)
                     /locus_tag="PW109_000098"
     CDS             complement(22689..24149)
                     /locus_tag="PW109_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2509983.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24139..25434)
                     /locus_tag="PW109_000099"
     CDS             complement(24139..25434)
                     /locus_tag="PW109_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2509984.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25766..26443)
                     /locus_tag="PW109_000100"
     CDS             complement(25766..26443)
                     /locus_tag="PW109_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2509985.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26473..27564)
                     /locus_tag="PW109_000101"
     CDS             complement(26473..27564)
                     /locus_tag="PW109_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2509986.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27665..28099)
                     /locus_tag="PW109_000102"
     CDS             complement(27665..28099)
                     /locus_tag="PW109_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2509987.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28683..30140)
                     /gene="ascB"
                     /locus_tag="PW109_000103"
     CDS             complement(28683..30140)
                     /gene="ascB"
                     /locus_tag="PW109_000103"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2509988.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30137..32032)
                     /locus_tag="PW109_000104"
     CDS             complement(30137..32032)
                     /locus_tag="PW109_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2509989.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32090..33865)
                     /locus_tag="PW109_000105"
     CDS             complement(32090..33865)
                     /locus_tag="PW109_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2509990.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33915..35411)
                     /locus_tag="PW109_000106"
     CDS             complement(33915..35411)
                     /locus_tag="PW109_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2509991.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35605..37227)
                     /locus_tag="PW109_000107"
     CDS             complement(35605..37227)
                     /locus_tag="PW109_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2509992.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37555..38868)
                     /locus_tag="PW109_000108"
     CDS             complement(37555..38868)
                     /locus_tag="PW109_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2509993.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39113..39337)
                     /locus_tag="PW109_000109"
     CDS             complement(39113..39337)
                     /locus_tag="PW109_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509994.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39357..39779)
                     /locus_tag="PW109_000110"
     CDS             complement(39357..39779)
                     /locus_tag="PW109_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2509995.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="PW109_000111"
     CDS             complement(39922..40632)
                     /locus_tag="PW109_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2509996.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="PW109_000112"
     CDS             complement(40650..41147)
                     /locus_tag="PW109_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2509997.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="PW109_000113"
     CDS             complement(41184..41636)
                     /locus_tag="PW109_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2509998.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="PW109_000114"
     CDS             complement(41771..42376)
                     /locus_tag="PW109_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2509999.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="PW109_000115"
                     /pseudo
     CDS             complement(42826..43446)
                     /locus_tag="PW109_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(43448..44317)
                     /locus_tag="PW109_000116"
     CDS             complement(43448..44317)
                     /locus_tag="PW109_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2510000.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="PW109_000117"
     CDS             complement(44340..44747)
                     /locus_tag="PW109_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2510001.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="PW109_000118"
     CDS             complement(44766..46055)
                     /locus_tag="PW109_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2510002.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46082..46384)
                     /locus_tag="PW109_000119"
     CDS             complement(46082..46384)
                     /locus_tag="PW109_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2510003.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46409..48502)
                     /locus_tag="PW109_000120"
     CDS             complement(46409..48502)
                     /locus_tag="PW109_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2510004.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48991..49755)
                     /locus_tag="PW109_000121"
     CDS             complement(48991..49755)
                     /locus_tag="PW109_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2510005.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49866..50735)
                     /gene="mdcB"
                     /locus_tag="PW109_000122"
     CDS             complement(49866..50735)
                     /gene="mdcB"
                     /locus_tag="PW109_000122"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2510006.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50760..51836)
                     /locus_tag="PW109_000123"
     CDS             complement(50760..51836)
                     /locus_tag="PW109_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2510007.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51860..52453)
                     /locus_tag="PW109_000124"
     CDS             complement(51860..52453)
                     /locus_tag="PW109_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2510008.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52457..54133)
                     /gene="mdcD"
                     /locus_tag="PW109_000125"
     CDS             complement(52457..54133)
                     /gene="mdcD"
                     /locus_tag="PW109_000125"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2510009.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54123..54422)
                     /locus_tag="PW109_000126"
     CDS             complement(54123..54422)
                     /locus_tag="PW109_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2510010.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54524..56170)
                     /gene="mdcA"
                     /locus_tag="PW109_000127"
     CDS             complement(54524..56170)
                     /gene="mdcA"
                     /locus_tag="PW109_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2510011.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56177..57088)
                     /locus_tag="PW109_000128"
     CDS             complement(56177..57088)
                     /locus_tag="PW109_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2510012.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57208..58065
                     /locus_tag="PW109_000129"
     CDS             57208..58065
                     /locus_tag="PW109_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2510013.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58252..58614)
                     /locus_tag="PW109_000130"
     CDS             complement(58252..58614)
                     /locus_tag="PW109_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2510014.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            58960..60399
                     /locus_tag="PW109_000131"
     CDS             58960..60399
                     /locus_tag="PW109_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510015.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60406..62958)
                     /locus_tag="PW109_000132"
     CDS             complement(60406..62958)
                     /locus_tag="PW109_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2510016.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63072..64403)
                     /locus_tag="PW109_000133"
     CDS             complement(63072..64403)
                     /locus_tag="PW109_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2510017.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64369..65847)
                     /locus_tag="PW109_000134"
     CDS             complement(64369..65847)
                     /locus_tag="PW109_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2510018.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66152..67987)
                     /gene="tdc"
                     /locus_tag="PW109_000135"
     CDS             complement(66152..67987)
                     /gene="tdc"
                     /locus_tag="PW109_000135"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2510019.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19402 a        14564 c        11677 g        22584 t
ORIGIN      
        1 tttttagaaa aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa
       61 agagctttgg tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag
      121 tcgttggatt tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag
      181 ggattataaa aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata
      241 gtttatctat ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt
      301 tgactggagg agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa
      361 aaaaagatga actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt
      421 caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct
      481 atttatttga tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag
      541 aagttgaaaa attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg
      601 aagtcgagcg actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag
      661 gaacactcga tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag
      721 agaatagtca cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg
      781 ctcaatttgc tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag
      841 gtgtgaaaaa atttgaatct attccaccga ctcttgagga tttattcatg agacattatg
      901 aaggatgagg aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc
      961 aaaaccttag ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt
     1021 ctggtggttt tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat
     1081 acgaaacctt gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg
     1141 ctgctgagta tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt
     1201 ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg
     1261 gtctgattga acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca
     1321 tcgctgaaag tattctgatc aatttgcttt tagcaatatc gatcagttgt acgatgattt
     1381 ttttccaagc ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta
     1441 gtatggggat tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag
     1501 ccagttcgaa tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa
     1561 cagatgtagc tgattcctcc ctttcgatgt ggaaaccttt aggctggtct tatttgacct
     1621 atccatttac aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct
     1681 tgataatcag ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg
     1741 aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct
     1801 atctaaataa agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat
     1861 acggctctat ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga
     1921 tgtttatcca atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct
     1981 tgatttgtct agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa
     2041 cccacggacg tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga
     2101 ccaatatgtt actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac
     2161 tagggctttc tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc
     2221 ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc
     2281 tattcggatg gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg
     2341 cactcaatta ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc
     2401 ctagctgggt tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat
     2461 catgtatcag ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac
     2521 tagaaggata ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa
     2581 tccaacattt ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat
     2641 ccagacttct cgattctttc tttttacata accattctcc tctaataatt taaattttct
     2701 agataaagtt tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag
     2761 gaaaaagtga tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc
     2821 ttctactttt tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac
     2881 gttgattgct aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc
     2941 tttttgtctc aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt
     3001 tggaacgcct agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag
     3061 ctgttccttt ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac
     3121 taattgaggt tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt
     3181 gacaagtgcg tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa
     3241 tgaaacacat aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat
     3301 aaagaaacat aacttccctt attccaactg ataattttct tttatcatag cataaacgtc
     3361 aagcgaacca gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct
     3421 tcttttgaga ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata
     3481 atcaaaaaca aaccttcact cgcaggccga gagtaaggtt tgtttttcgc attgcttatt
     3541 tcgtgttgtc tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg
     3601 taaggtttca gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt
     3661 acccatttga aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc
     3721 ggacaaatag tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat
     3781 gtaataatcc ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt
     3841 atacgctgca atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat
     3901 ttggtattgt ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc
     3961 gcttcggtac gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat
     4021 tttaggtttg ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc
     4081 tttccatttt tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg
     4141 atggctctct ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga
     4201 ttttccaagt tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg
     4261 cgatatggac aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca
     4321 aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca
     4381 tctaaaagcg ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt
     4441 gcacgctctc ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct
     4501 tttgcctggt caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc
     4561 agccaaacat ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc
     4621 gctgttccct gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct
     4681 tttgtcgctt tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg
     4741 actttcgata tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga
     4801 tcggcttgaa aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct
     4861 ccatcgccaa tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct
     4921 gcaaattgcg tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt
     4981 tatcttgaga ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat
     5041 tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata
     5101 aaattaccga cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca
     5161 tcattcatga acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca
     5221 cctgcttctt ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc
     5281 agtactttag aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca
     5341 ccatactcga tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt
     5401 gctgctcgaa gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct
     5461 ttcaactcag ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg
     5521 acagtacctg caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca
     5581 tcatctacga tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata
     5641 tcgaagttca atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta
     5701 tcggcttcct taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca
     5761 agcaccgctg cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg
     5821 cttcctccac cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg
     5881 gaagattcta cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct
     5941 tcgacaatat tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc
     6001 ggcattttgt gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc
     6061 tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc
     6121 ttcttccaac atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa
     6181 aacaaaataa ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc
     6241 tgatccgatc tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg
     6301 catctgctca atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa
     6361 aatacttttt ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc
     6421 tttcattaat gaacaattca agtatataat aaatttctaa ttttgggaaa atatgtgtag
     6481 tgcttcttga agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca
     6541 acaacgaaca gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag
     6601 gtcattactg ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc
     6661 ggccgctcaa aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa
     6721 atggccgaac gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat
     6781 gaagtattag gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc
     6841 acgagataag taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca
     6901 ctcaagaatt agatttttca gtctaacttc gaggtactct accttcccat cctctctttt
     6961 ttatttcttt ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa
     7021 ggaataactg acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt
     7081 ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc
     7141 tcctgccgaa tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt
     7201 tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact
     7261 ggccgctgtg atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg
     7321 atttaattgc tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac
     7381 agcttccgct tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc
     7441 cactttattt ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt
     7501 gttttttgca gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa
     7561 atgatcgatt tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat
     7621 cgtttctcct gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt
     7681 ttcagcacca gaagagctta tggttgttga aggttctgtt tgagcaggtt cagaagtttc
     7741 cgcaaaagct gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg
     7801 cttcattctt tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta
     7861 taagcaaaaa tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc
     7921 atcttttatt tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc
     7981 gttttgactg ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa
     8041 agcatatata gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc
     8101 agcgaagcta gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg
     8161 aatgtaaaag taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat
     8221 ccagcaaaaa gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga
     8281 tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat ggagcctcac ttttttgaaa
     8341 ttcgtcagcc agacttctgt tccacctttt gcccaacgtt ttctgtgtcg atatagcatc
     8401 ttcaacgtca caggtacttc catgaaaaag ataatctcag gagcaaaaac agataaccag
     8461 tcatttaact gatgatccca agcaatactg atgtcttctg tcgctcgatc ttgtctgaat
     8521 ccgcccacat cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa
     8581 agaccaccga aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca
     8641 accgtttgag attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc
     8701 gcagcaatat gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct
     8761 ggctccggta ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt
     8821 ccgatattaa acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta
     8881 gcgtattttt tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct
     8941 gtcactaata cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatat
     9001 tcgatcacaa tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc
     9061 aagggaacat ctacccattc tttttgctta tgtttgtata aaaacacata acaccagaca
     9121 ccaataaacc atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa atacataata
     9181 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
     9241 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
     9301 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
     9361 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
     9421 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
     9481 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
     9541 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
     9601 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
     9661 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
     9721 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
     9781 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
     9841 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
     9901 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
     9961 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    10021 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    10081 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    10141 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    10201 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    10261 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    10321 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    10381 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    10441 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    10501 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    10561 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    10621 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    10681 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    10741 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    10801 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    10861 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    10921 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    10981 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    11041 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    11101 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    11161 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    11221 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    11281 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    11341 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    11401 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    11461 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    11521 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    11581 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    11641 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    11701 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    11761 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    11821 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    11881 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    11941 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    12001 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    12061 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    12121 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    12181 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    12241 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    12301 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    12361 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    12421 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    12481 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    12541 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    12601 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    12661 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    12721 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    12781 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    12841 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    12901 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    12961 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    13021 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    13081 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    13141 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    13201 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    13261 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    13321 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    13381 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    13441 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    13501 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    13561 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    13621 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    13681 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    13741 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    13801 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    13861 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    13921 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    13981 tatatgcccg ccccggtatt ctgcaggtgc tctaacatca agtaacgctg gattttccaa
    14041 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    14101 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    14161 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    14221 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    14281 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    14341 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    14401 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    14461 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    14521 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    14581 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    14641 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    14701 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    14761 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    14821 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    14881 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    14941 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    15001 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    15061 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    15121 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    15181 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    15241 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    15301 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    15361 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15421 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    15481 caaaaacaac ttctcctgtt tttggttcgg ttggcttatt atcgagaagt agctactggg
    15541 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    15601 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    15661 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    15721 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    15781 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    15841 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    15901 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    15961 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    16021 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    16081 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    16141 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    16201 aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat gagctaattt
    16261 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    16321 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    16381 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    16441 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    16501 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    16561 caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc ttcctatttg
    16621 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    16681 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    16741 cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag tgcttcccga
    16801 tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt cttgatttct
    16861 ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc catgattgcg
    16921 atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc taaaagtacc
    16981 aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg accagaaaat
    17041 gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat ctcatcaaag
    17101 acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc catcaaagtc
    17161 aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc ggtgataatc
    17221 aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc aggtaaaaat
    17281 ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg tgtgcttcca
    17341 ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt gatcgtttcg
    17401 atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc cgccaggact
    17461 tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt caatttagaa
    17521 atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat aatcgttcca
    17581 tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc tttttgaaca
    17641 atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt cagtaatttt
    17701 gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa attacgtgac
    17761 gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc tacatttatc
    17821 aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg attcttatca
    17881 aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat caaatcggtt
    17941 ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct cggcaacccc
    18001 ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt ttcttcttga
    18061 cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg ttttttgcta
    18121 tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg taattgacta
    18181 atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc aaaataaata
    18241 agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt cctatcctta
    18301 ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca ccaaataaac
    18361 tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca aaacataccc
    18421 cattcttcta ggtcttattt attttccact taactatagc aagacacatt ttggttgtca
    18481 aactttagta tgttttatag atttacacca ttaatgaata gacacctacc gcgataattg
    18541 ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa gttcctttat
    18601 tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca gggttgattg
    18661 catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca cctacagcga
    18721 atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca agaactaata
    18781 caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata gcaggacctg
    18841 ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt ggcaaataag
    18901 aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg atgaaaggaa
    18961 cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact gcaggattca
    19021 aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg agtccccaac
    19081 caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa ttcacagccg
    19141 cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc cccatgattt
    19201 gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct gattctgcaa
    19261 tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat tcaaaatatc
    19321 cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt tcaaacaaca
    19381 gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct tcttctaaac
    19441 cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc atttcttttg
    19501 ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa gtagcatctt
    19561 cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt tcttctaatt
    19621 tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt tctttcgtct
    19681 ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct tctgccatct
    19741 tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt tctctttcta
    19801 aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt tccaaatggt
    19861 tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca cttactgtat
    19921 cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca ttgtaatcag
    19981 aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg atatcattca
    20041 atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa tctacatctt
    20101 cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc gtaccaaagt
    20161 aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt cctgtatcga
    20221 agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg atccctttag
    20281 ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact ttatctaccc
    20341 aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca tctgtcagca
    20401 ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca accgctttca
    20461 ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca atcactaaac
    20521 gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct tcttttttca
    20581 aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca tatttggttc
    20641 ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag aacatattga
    20701 aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt tttgggatgt
    20761 gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact acttccacat
    20821 caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga gaggaagtcc
    20881 cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct gctgactgga
    20941 ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat tcttcttgct
    21001 gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt tcctccttat
    21061 tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt ttctcggcgc
    21121 tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg gatctcgtct
    21181 gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag cgccgttgtt
    21241 tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa ttgcatcaga
    21301 tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat tccagtgtca
    21361 tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa agtagcttta
    21421 atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg tgctgcctga
    21481 tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc atcttcgctt
    21541 gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg aagtccatct
    21601 ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc atagtaaacc
    21661 tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa ttgcggtttt
    21721 tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac catgcctggt
    21781 tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc tgaaattggt
    21841 gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga gttagaagtg
    21901 acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg atcccaatct
    21961 agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt aacatgtgct
    22021 tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat caattcgcct
    22081 ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat cttcgttgct
    22141 gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg atagccttca
    22201 ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac tgcatgatag
    22261 attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt cgttatgcca
    22321 atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc aataactgac
    22381 tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg cgggaaatat
    22441 tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa gataattgct
    22501 cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt cattgtgatt
    22561 ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg ttttaaaaaa
    22621 agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat tatgaagcta
    22681 ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa tcgaataatg
    22741 tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt tctctggcaa
    22801 aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc aataccgccg
    22861 attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat cggcagactt
    22921 tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat caaaagaaca
    22981 ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt cctcggcaat
    23041 tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg cccgagcatt
    23101 ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc aacaaagaaa
    23161 aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc tttgattttc
    23221 aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc taaaacagcg
    23281 gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc gtctactatc
    23341 gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag ttctgctgct
    23401 gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg gtccgactga
    23461 tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa tagtcttttt
    23521 ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa cgttgtttct
    23581 aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg caaaaaaagc
    23641 ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg tatctgtagc
    23701 tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa gagctggttc
    23761 aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt cgctaatcga
    23821 acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt caagctttcc
    23881 tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac aagcagcgcc
    23941 tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat caattcaata
    24001 tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct caaaccattg
    24061 atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg taactgctct
    24121 tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg accacaccat
    24181 atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact gttcaaacgt
    24241 ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct cttttttgct
    24301 ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga gtttaacttt
    24361 gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt tttggggata
    24421 aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa accactgatt
    24481 gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg tcttgctgac
    24541 atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt catttgttgg
    24601 acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag acgtttgcaa
    24661 gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt cccacatctg
    24721 attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct ttcttttttc
    24781 cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt ctaaaaaaag
    24841 tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag gatctagata
    24901 acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc ttgatgccaa
    24961 cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt tctttgcatt
    25021 catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt gaataagcgt
    25081 tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa tcaaacgatc
    25141 aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc ctattactgt
    25201 cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg tgatccaaga
    25261 aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc tgggaatcca
    25321 tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca attggcgaag
    25381 ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt tcatctgcat
    25441 tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac agtattcata
    25501 actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct ccatcatacc
    25561 gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag tatcatttct
    25621 gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt ttacgtacct
    25681 ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg taatgtaata
    25741 gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact gcttcgccta
    25801 attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt tgaaccactt
    25861 ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca tcttctgcta
    25921 agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg aacccagtca
    25981 ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac aatcctacaa
    26041 caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa gcgactttcc
    26101 caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca taataaaccg
    26161 ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag gcccgatttc
    26221 tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa acactttgcg
    26281 gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc ccatttttta
    26341 ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga cgctcgatct
    26401 tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca tcctctttct
    26461 ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc ttggcaaata
    26521 aagctcttta tatgcttctt caaaaggttt cgttacaaat tcatcctcac ggttttccaa
    26581 taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg caagttctgc
    26641 atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac caactaaagg
    26701 gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt tgtatttatc
    26761 agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta gttttgactc
    26821 acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg tttcatattc
    26881 ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt caaccgctga
    26941 gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc taccgcccca
    27001 ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat acgtatttgc
    27061 tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg ctttgccaaa
    27121 tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt ccgcatcagg
    27181 acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag ttgctcgact
    27241 tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc cttggattgc
    27301 tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga gttcacctgg
    27361 ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac caagaccttt
    27421 agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga aaaagctgag
    27481 ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg aaggtaaagt
    27541 aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg attaacctat
    27601 cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat ttgcttaaaa
    27661 aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct aattctttac
    27721 ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag ctaccatcga
    27781 cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag cgaccttcgc
    27841 taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca atcgttgatg
    27901 gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt tcttttaata
    27961 tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct tcatcagcta
    28021 atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca tctaacgctt
    28081 tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag tatgattttt
    28141 attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt gaaaaaaaga
    28201 agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg gaacaccgtg
    28261 aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt tgtgaaaatg
    28321 gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt tgaacaccgt
    28381 ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag ccaaacaaaa
    28441 atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg attgtacacc
    28501 gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc aatttcaaaa
    28561 aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg caggagaata
    28621 ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct tatttccttt
    28681 tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa tgattttttc
    28741 tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat atatccataa
    28801 cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca cgacgtataa
    28861 ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg ctgcttcaaa
    28921 tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc tatagctccg
    28981 agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg attcaaagca
    29041 attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag atacggattt
    29101 ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg atgagcactg
    29161 actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc tgctaagatt
    29221 tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat tcgttgcgta
    29281 tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt tctttgctgc
    29341 tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag ctgatagctg
    29401 atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt agctaaagca
    29461 cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg gggatggttt
    29521 tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat ctcattgaaa
    29581 gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc gaaacgaacg
    29641 taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa atataacggc
    29701 atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca ttcgtcaaac
    29761 acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc atgaggaaaa
    29821 attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc aaataaacgg
    29881 atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata ataatacttg
    29941 ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca ttgatctgtc
    30001 catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga taatcctttt
    30061 tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc ccataaaaat
    30121 ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt gtttgattat
    30181 ttttcttatg actagtaatg atatttttga atctactact atttgtaaca ataaccattg
    30241 tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc aataaatcgc
    30301 ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca tttaaattca
    30361 ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact accccaattg
    30421 catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag atttttccgt
    30481 ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt ggatcgttta
    30541 cttcagataa atcaattatt tcacctgctg caggagtact taacactata tcctttgagg
    30601 attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag gaactgatca
    30661 tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa atcgggaaag
    30721 acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca gctactccac
    30781 caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta agagtcacac
    30841 cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat aaagataaac
    30901 tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct gcttgcgcag
    30961 ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa agattgttgg
    31021 tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc caaaatccgc
    31081 cgataacagc accagataaa acagggctaa agttataaac agctaaatat ccagctgcaa
    31141 tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt gggaccatca
    31201 cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag cttttgcgaa
    31261 tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata accgtacttg
    31321 tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta tcactatatg
    31381 cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg aaacgatctg
    31441 tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag aaaaatgcat
    31501 cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa ttcaaactcg
    31561 tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct aaagtcgggg
    31621 caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc tcagttcctc
    31681 tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg tcatacactt
    31741 catttactgc atttcctact acgacttgaa acattcctcc agcttcgact acagtgatca
    31801 caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt agggtaaagc
    31861 gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg acaaatcgga
    31921 caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta attaatgccg
    31981 tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc attcaattag
    32041 taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact tagccgagca
    32101 aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca aaggagacaa
    32161 aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc tgatactctg
    32221 tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg gcatttaacg
    32281 gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct atctttttat
    32341 ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt ttcttttttt
    32401 cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat tcaaactcat
    32461 ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct tgctcagtag
    32521 tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga tacactggtt
    32581 ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt tttagtgtat
    32641 tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt cgaaaaggat
    32701 tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg ggacgtaatt
    32761 gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa cgggagatga
    32821 tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt ttcatttgtt
    32881 tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga agatctaaaa
    32941 aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg tttttttcat
    33001 cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt ttttcttgat
    33061 tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct accatcggaa
    33121 taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga atggtcaaaa
    33181 cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta tatgatataa
    33241 gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt gtactgattt
    33301 gttgaggcaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat aagaaaattc
    33361 tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact tccagttcta
    33421 atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat acggctgttt
    33481 ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt tgaaacaata
    33541 agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta tgtttcgtca
    33601 cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa taacctgaac
    33661 gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt ttgatttcag
    33721 tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt gttatccaat
    33781 gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag tccatttgct
    33841 tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa tagtcacttg
    33901 cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc attagactgg
    33961 ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct tccgctccca
    34021 tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc tgttcctgcc
    34081 gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc atcttttatt
    34141 gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg atcgtggatt
    34201 cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat gaataatggt
    34261 aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg atcaattggc
    34321 caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc atttgctccg
    34381 ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata ataactaaat
    34441 gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa cattactggt
    34501 gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc tcggcacatg
    34561 acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac attctctgga
    34621 tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc catcttagca
    34681 tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa catatgatga
    34741 caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt atctctagca
    34801 gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt gatctcatta
    34861 aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg cgcataaaat
    34921 tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac caatgctaag
    34981 ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt taatgtattg
    35041 attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc tcctcttgga
    35101 aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc cttcagtaaa
    35161 cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag ataatggagt
    35221 gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat actaggatgc
    35281 tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc ttccaaccat
    35341 gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc tggaaatatt
    35401 tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag tatagctcga
    35461 ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa ttgaatgcta
    35521 ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga aagaaaacac
    35581 tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt cataaggtcg
    35641 taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa taacccttaa
    35701 agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa taacgagcca
    35761 ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct tttccactaa
    35821 ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca atttcttgta
    35881 catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt ctttataatt
    35941 tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac gggcgttcca
    36001 ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca agatttcctc
    36061 ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa tatcttttgc
    36121 ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt gatagatata
    36181 aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt cttttcgata
    36241 cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat tccaaaataa
    36301 actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt ttttcagttc
    36361 agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat ctaatcctat
    36421 gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat aaagtttagc
    36481 gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt caccaaaagt
    36541 tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg tgatttcttt
    36601 atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa tacctttatc
    36661 gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt tccaattcaa
    36721 atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt catccaactg
    36781 ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat tgaccggatc
    36841 acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct caatgaacca
    36901 cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga tatttctttt
    36961 ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg ccatgattgc
    37021 ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata ctggagaaag
    37081 ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa tcccccgcaa
    37141 atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt gataaacaac
    37201 tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg tttctatttt
    37261 ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc aaatagctat
    37321 tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat tccctcaact
    37381 gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact tctttataaa
    37441 cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata taagaaacta
    37501 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    37561 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    37621 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    37681 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    37741 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    37801 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    37861 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    37921 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    37981 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    38041 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    38101 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    38161 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    38221 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc cgatgaagat
    38281 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    38341 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    38401 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    38461 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    38521 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    38581 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    38641 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    38701 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    38761 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    38821 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    38881 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    38941 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    39001 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    39061 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    39121 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    39181 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    39241 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    39301 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    39361 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    39421 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    39481 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    39541 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    39601 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    39661 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    39721 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    39781 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    39841 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaattactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa atcaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta
    46081 tttagataac tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg
    46141 aaacaatatt tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact
    46201 gcgaacgaaa attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa
    46261 tatcacaatg gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca
    46321 tctgtgctgc aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt
    46381 tcatctttgt tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt
    46441 tgaaatcttc gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca
    46501 attcaattag ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa
    46561 aaataatctt tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag
    46621 ctacgctgac tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt
    46681 gtttgccaat cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat
    46741 agcgctcttc gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct
    46801 cccaagtagt ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca
    46861 taggctggat ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact
    46921 tgccatagat gattcgatct actcggccac cactttcttt catgacatct attatttgag
    46981 tgaataactc cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt
    47041 tcaaaaagtc ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa
    47101 gcggcttatt tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat
    47161 ctactaattc gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt
    47221 ctttcagatt ctctgctaac agatttccag tagccatccc atggttacag acaacgactg
    47281 atttataaac ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga
    47341 caagaaaagc tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa
    47401 caaaatgagt gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg
    47461 gattcatgac ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga
    47521 gattttcgaa taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa
    47581 attggatcaa ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat
    47641 ctctagcatt gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat
    47701 tgtccaattg aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta
    47761 tttctgtatt tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc
    47821 agatcaatgt aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact
    47881 tattggaata gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga
    47941 ttttttgagc cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat
    48001 tctgattgat aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca
    48061 gtccttgctt tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc
    48121 gtctcatatc ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta
    48181 gatggatcgg gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc
    48241 gattcaaaaa gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct
    48301 tttccagctt caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac
    48361 tgctcaacca gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga
    48421 actcacttgc tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta
    48481 tttcaattgt tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg
    48541 cttacataat aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca
    48601 aacaaaaaat aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat
    48661 tacaaaaatt ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag
    48721 taataagaac aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat
    48781 cgcagatgca agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct
    48841 tgagcgataa gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat
    48901 tgccgatagg ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt
    48961 tgtcacaact ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc
    49021 ggcttgccat cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg
    49081 tcgttgtgtc ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca
    49141 gcatctgaaa tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac
    49201 ttcctgaata gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag
    49261 aactttctga tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt
    49321 ttcttcttcc caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc
    49381 tttcttttct ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt
    49441 ttgtctttta ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa
    49501 tgcaagaaat cccgtttgct cataactaga aggacctgta cctggaccat aatacccata
    49561 agaatctctg ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa
    49621 tgaacctctg cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc
    49681 gaatccttgg gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata
    49741 attgattttt ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata
    49801 gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt
    49861 cttatttagt cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct
    49921 atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg
    49981 ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta
    50041 gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg
    50101 catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat
    50161 gaatcattat ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat
    50221 atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg
    50281 gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt
    50341 attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat
    50401 agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt
    50461 gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca
    50521 tgatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta
    50581 agaaataaag catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca
    50641 ggttttggac ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc
    50701 tgagtcactg ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat
    50761 tacttgctga aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca
    50821 aatgcacaac ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc
    50881 agccaactat ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac
    50941 gtacgataag cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca
    51001 atcattttgg aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg
    51061 atcgtattca agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa
    51121 cggacagaaa agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc
    51181 aaatattgat agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta
    51241 gcaacaaacc gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt
    51301 tgatagaaca gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca
    51361 tattttttat tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg
    51421 atctgcagca tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc
    51481 agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa
    51541 agataagccg tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa
    51601 aattcccaat aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata
    51661 aacattggtg ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt
    51721 gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca
    51781 gctgatactt tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct
    51841 ttctctctct ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt
    51901 tcaatagaat ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc
    51961 gagtcactac ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa
    52021 ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta
    52081 ctgccggaat cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc
    52141 ctgataatat gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct
    52201 cttgtctata cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat
    52261 gttttgagat ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta
    52321 gtatttctgc gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa
    52381 cgacccattc aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat
    52441 gtgcttgaat cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg
    52501 tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt
    52561 catttgtatt gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat
    52621 tttgttctga agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt
    52681 ttgtataatt attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt
    52741 cagcaaccga acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa
    52801 tttctttatt gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac
    52861 ctgaaatagc atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg
    52921 cataagcagc agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc
    52981 cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt
    53041 tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa
    53101 cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga
    53161 tggcttcttc tatgaattta cttgatttac cataatacac agtactaata gaagaagttg
    53221 gatttttcat gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt
    53281 catttccttc tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt
    53341 cattgtaatc gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat
    53401 aaaatgctga gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag
    53461 cagctttgat tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc
    53521 gttgacgaga tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa
    53581 cgcctgcttc ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg
    53641 cgattaaata actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc
    53701 ctggaatcaa accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt
    53761 ctgaaataga taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta
    53821 caatgactgg aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag
    53881 caacgatttt tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag
    53941 cgattactac tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac
    54001 tttcagggac gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa
    54061 tcaattcacg tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa
    54121 aactattttt catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct
    54181 ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat
    54241 ctcgttaata cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca
    54301 gtagattctg ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact
    54361 cctacatgga ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc
    54421 atggatctct cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc
    54481 ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga
    54541 atttcattgg tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg
    54601 cagctaataa tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca
    54661 cgatgccggc tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg
    54721 taacaccgcc aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca
    54781 aataagcaat tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg
    54841 gtgcattggc taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga
    54901 ctggtttttt atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc
    54961 ctaatggatc atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc
    55021 caggattatg tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg
    55081 tagaagagtt tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat
    55141 attgtcctgc agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa
    55201 aaaagacatc tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat
    55261 ggatgctttc aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata
    55321 cccagttctt cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca
    55381 attcgatagc tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg
    55441 cgtagattcc tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag
    55501 gatctcgtgt gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta
    55561 cccagtcacc agggatatct acacgtggta aagaatcgac aacttcgttg acttgaacga
    55621 tcacgatacc atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga
    55681 aacctgtgta caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg
    55741 gaatcaaatc gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt
    55801 tcattgtgcc gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga
    55861 aatcgatctt gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac
    55921 tagacatgat caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg
    55981 aaagaaaact tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa
    56041 tcagtttttc cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt
    56101 tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg
    56161 gtttttccat aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat
    56221 gttggtctag attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct
    56281 ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca
    56341 agtccatcat ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa
    56401 tctcttctgc gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat
    56461 cagatagatg gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga
    56521 gacagtgaga tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg
    56581 cttctgccaa cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac
    56641 aattttcatt tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct
    56701 cagcttcgct tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt
    56761 ctttcattaa gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc
    56821 gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa
    56881 taccgttttt ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga
    56941 taaaaaccgt atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt
    57001 ctaaacgttc catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt
    57061 gtccaggaaa taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca
    57121 ctgctatctt agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac
    57181 aaaaaggtat ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa
    57241 gtattcgaaa caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc
    57301 ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc
    57361 ggcagaaaca ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca
    57421 ttagacacat tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact
    57481 tccgtcatat tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt
    57541 atggaaaagc atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt
    57601 accgatcctg tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc
    57661 atgaaagaac ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat
    57721 ctgcaattag aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc
    57781 cgtcatacac tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg
    57841 acagatgatc ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc
    57901 ccaaaagttt cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat
    57961 ttgcccttga cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc
    58021 aaaatgattg caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg
    58081 gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta
    58141 gaaaataagc cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    58201 tcttgaagct aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt
    58261 tgatttaaaa cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag
    58321 atcaatagag atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc
    58381 tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg
    58441 gtcacttgca tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt
    58501 tttgcttttt tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg
    58561 agacaaattg tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt
    58621 ccatctcttt cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa
    58681 tacaagtagt tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt
    58741 caatccgcct gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc
    58801 acctattttt tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca
    58861 gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt
    58921 attttctccg tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa
    58981 atcattaccg aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat
    59041 acaaagataa ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt
    59101 tctgaccttc aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa
    59161 gcaggaatca gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt
    59221 ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct
    59281 gtccctgatt ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact
    59341 aaaatcaatc agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct
    59401 tgtattcttg gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc
    59461 actcataaca actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt
    59521 ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt
    59581 tggttcttta tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat
    59641 accaacagca cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa
    59701 ggtatttatc ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg
    59761 aattacaaca ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta
    59821 gaggtcctcg aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa
    59881 aaaacatctc tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata
    59941 ttgattgaag cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat
    60001 gcacacttgt atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc
    60061 aatgaaccaa attactttct ccaactcttg tctctactga tccaacaaac gattttttct
    60121 atcaaaccga atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg
    60181 ttcttgcagc aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa
    60241 ctaaccgatc tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct
    60301 ctagattctc tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc
    60361 aatcgtttga ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc
    60421 tctagctgtt ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac
    60481 aactaagaat agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact
    60541 tactgtaccg atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc
    60601 aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg
    60661 cttggacaac tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag
    60721 aagaatggtt tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac
    60781 catgatcagt agggagacat agatgatccg aaacaaatga taaccagata ggattccttg
    60841 ctttggatct cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc
    60901 ataagataaa gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg
    60961 tagcgggcga ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg
    61021 caagaagaaa ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc
    61081 ttttgcaatc gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac
    61141 ttcactacct ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc
    61201 atttacgccg tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat
    61261 tcgcagttta tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat
    61321 caactcttca tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt
    61381 taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt
    61441 cactgagatc cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg
    61501 atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc
    61561 ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa
    61621 ttctgcagct gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa
    61681 taatacttct ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt
    61741 tgccatatat ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag
    61801 attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc
    61861 tgttttctgt tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca
    61921 ggcttcttct tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt
    61981 cttatctgaa cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat
    62041 gatcgccttt tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc
    62101 tggcaatcct tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaaggatatg
    62161 ccaatccata ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat
    62221 gccttgaaaa atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg
    62281 ttcgaccgat tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac
    62341 aacgacaccg actgcagaac cagattgtac aagcgttcct gaaaaaccca tatttttctg
    62401 gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc
    62461 tcccgtcaaa atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc
    62521 tggaacgaca tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg
    62581 gattttttct tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa
    62641 accattcaat gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat
    62701 cactacgaat aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga
    62761 ggcaatgatc aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg
    62821 ttttgtctgc tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg
    62881 ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt
    62941 caatctgtac cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg
    63001 gaagtttttt caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa
    63061 tgtatctggg cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat
    63121 acaaaccaga taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg
    63181 aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc
    63241 cgtttctttt ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga
    63301 aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa
    63361 aaatgtagac aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt
    63421 acgcctttct tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga
    63481 tataaaatcc tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa
    63541 atcattagat tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact
    63601 aaaggacttt cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc
    63661 gttcctatat agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc
    63721 ttttgcggat cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg
    63781 aataaaacca taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg
    63841 atctgtccaa aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat
    63901 cctataccca ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat
    63961 tccaattcac caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg
    64021 ctacctccgc ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt
    64081 gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa
    64141 aatgcccact ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta
    64201 tttgctaaaa ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc
    64261 aaataaacga gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat
    64321 aatcctgttc caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc
    64381 cgtttcattg ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac
    64441 ttcctgttcc caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat
    64501 gacactgatg gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc
    64561 gacagaaaat ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct
    64621 ctttttctct ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa
    64681 tctcaaaact gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt
    64741 tacgagcatc aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat
    64801 tgagataatg acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt
    64861 ccaaaaattg aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc
    64921 tggtcctgag gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa
    64981 cagattcaca ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc
    65041 gatattattc aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa
    65101 aatattgacg aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac
    65161 tggattttcc aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat
    65221 gatcggcgca aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca
    65281 tgaaaaactg cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc
    65341 taataagagc atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc
    65401 gatttttgcc atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat
    65461 gacaactaat gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa
    65521 caacggagcg agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag
    65581 ccatgagaca acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc
    65641 acctttttgg ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta
    65701 caataatact gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg
    65761 aatatttcgt acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat
    65821 ttgctttgat gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt
    65881 ttgttttctt ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa
    65941 aacccagtat caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa
    66001 acgaacgatc caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg
    66061 aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt
    66121 ctcttgttat tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt
    66181 ccattgcttt ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag
    66241 gagtcatgat agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg
    66301 ttagacccat tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg
    66361 tagtatgaga agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat
    66421 cgaacatttt ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga
    66481 atacccaatt gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc
    66541 ctttaacagt aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg
    66601 aagccccaat caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc
    66661 agactgcggc ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat
    66721 ctggagcctt tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca
    66781 tatttttatg tttgattacg attcctccag cagcataagg gacatagccc atcttatgag
    66841 gatctatcgt gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt
    66901 caatcgttac gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat
    66961 acggcacaaa ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg
    67021 catcaacatg taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga
    67081 ttttatctac actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta
    67141 gaattgggat tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca
    67201 ttcgataatc ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat
    67261 ctaacgcctt catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt
    67321 ggatattttt tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa
    67381 agctttcggt catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca
    67441 tttttttcac tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac
    67501 gggcgtacca taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc
    67561 cgtctgtcat attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag
    67621 ttgccataga agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag
    67681 ctaataaagc aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat
    67741 gccatgggat cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat
    67801 ggtcgcgggt ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat
    67861 ctgaaggaat ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca
    67921 ttttataaac tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat
    67981 ctttcatttt tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat
    68041 ctgtaacacc tcttcactat agcgccaaaa atgagaattt tttttttata acatcaaatc
    68101 cacgtagatt tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa
    68161 aaaagcgaac aatttttcaa taaatcagtc ttctaaaata gataacctaa aaaaagcgaa
    68221 caaacaa
//
LOCUS       DANKHK010000003        67926 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000003 DANKHK010000000
VERSION     DANKHK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(135..2321)
                     /locus_tag="PW109_000136"
     CDS             complement(135..2321)
                     /locus_tag="PW109_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2510020.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2539..3222
                     /locus_tag="PW109_000137"
     CDS             2539..3222
                     /locus_tag="PW109_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2510021.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3303..4022)
                     /locus_tag="PW109_000138"
     CDS             complement(3303..4022)
                     /locus_tag="PW109_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2510022.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4334..4540)
                     /locus_tag="PW109_000139"
     CDS             complement(4334..4540)
                     /locus_tag="PW109_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510023.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4785..5387
                     /locus_tag="PW109_000140"
     CDS             4785..5387
                     /locus_tag="PW109_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2510024.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5528..6808
                     /locus_tag="PW109_000141"
     CDS             5528..6808
                     /locus_tag="PW109_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2510025.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6821..7549
                     /locus_tag="PW109_000142"
     CDS             6821..7549
                     /locus_tag="PW109_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2510026.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7618..9141)
                     /locus_tag="PW109_000143"
     CDS             complement(7618..9141)
                     /locus_tag="PW109_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2510027.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9393..10574
                     /locus_tag="PW109_000144"
     CDS             9393..10574
                     /locus_tag="PW109_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2510028.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10634..12262)
                     /gene="treC"
                     /locus_tag="PW109_000145"
     CDS             complement(10634..12262)
                     /gene="treC"
                     /locus_tag="PW109_000145"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2510029.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12713..15424)
                     /locus_tag="PW109_000146"
     CDS             complement(12713..15424)
                     /locus_tag="PW109_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510030.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15434..16135)
                     /locus_tag="PW109_000147"
     CDS             complement(15434..16135)
                     /locus_tag="PW109_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510031.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16321..16920
                     /locus_tag="PW109_000148"
     CDS             16321..16920
                     /locus_tag="PW109_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2510032.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17001..18014
                     /locus_tag="PW109_000149"
     CDS             17001..18014
                     /locus_tag="PW109_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2510033.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18122..19039
                     /gene="rbsK"
                     /locus_tag="PW109_000150"
     CDS             18122..19039
                     /gene="rbsK"
                     /locus_tag="PW109_000150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2510034.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19067..19462
                     /gene="rbsD"
                     /locus_tag="PW109_000151"
     CDS             19067..19462
                     /gene="rbsD"
                     /locus_tag="PW109_000151"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2510035.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19462..20835
                     /locus_tag="PW109_000152"
     CDS             19462..20835
                     /locus_tag="PW109_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2510036.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21192..23336
                     /locus_tag="PW109_000153"
     CDS             21192..23336
                     /locus_tag="PW109_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2510037.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23388..24429)
                     /locus_tag="PW109_000154"
                     /pseudo
     CDS             complement(23388..24429)
                     /locus_tag="PW109_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(24706..26508)
                     /locus_tag="PW109_000155"
     CDS             complement(24706..26508)
                     /locus_tag="PW109_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510038.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26522..27274)
                     /locus_tag="PW109_000156"
     CDS             complement(26522..27274)
                     /locus_tag="PW109_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510039.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27283..27645)
                     /locus_tag="PW109_000157"
     CDS             complement(27283..27645)
                     /locus_tag="PW109_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2510040.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27821..28693)
                     /locus_tag="PW109_000158"
     CDS             complement(27821..28693)
                     /locus_tag="PW109_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2510041.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28744..29232)
                     /locus_tag="PW109_000159"
     CDS             complement(28744..29232)
                     /locus_tag="PW109_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2510042.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29370..30161)
                     /locus_tag="PW109_000160"
     CDS             complement(29370..30161)
                     /locus_tag="PW109_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2510043.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30216..30824)
                     /locus_tag="PW109_000161"
     CDS             complement(30216..30824)
                     /locus_tag="PW109_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2510044.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30817..31134)
                     /locus_tag="PW109_000162"
     CDS             complement(30817..31134)
                     /locus_tag="PW109_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2510045.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31290..32018)
                     /locus_tag="PW109_000163"
     CDS             complement(31290..32018)
                     /locus_tag="PW109_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2510046.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32177..33058
                     /locus_tag="PW109_000164"
     CDS             32177..33058
                     /locus_tag="PW109_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2510047.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33295..34338)
                     /locus_tag="PW109_000165"
     CDS             complement(33295..34338)
                     /locus_tag="PW109_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2510048.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34341..35009)
                     /locus_tag="PW109_000166"
     CDS             complement(34341..35009)
                     /locus_tag="PW109_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2510049.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35151..37487
                     /locus_tag="PW109_000167"
     CDS             35151..37487
                     /locus_tag="PW109_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2510050.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37602..37913)
                     /locus_tag="PW109_000168"
     CDS             complement(37602..37913)
                     /locus_tag="PW109_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510051.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38094..39569
                     /locus_tag="PW109_000169"
     CDS             38094..39569
                     /locus_tag="PW109_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2510052.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39616..40200
                     /locus_tag="PW109_000170"
     CDS             39616..40200
                     /locus_tag="PW109_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2510053.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40202..40621
                     /locus_tag="PW109_000171"
     CDS             40202..40621
                     /locus_tag="PW109_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2510054.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40902..41186)
                     /locus_tag="PW109_000172"
     CDS             complement(40902..41186)
                     /locus_tag="PW109_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2510055.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41418..42128)
                     /locus_tag="PW109_000173"
     CDS             complement(41418..42128)
                     /locus_tag="PW109_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2510056.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42125..43597)
                     /locus_tag="PW109_000174"
     CDS             complement(42125..43597)
                     /locus_tag="PW109_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2510057.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43735..44208
                     /locus_tag="PW109_000175"
     CDS             43735..44208
                     /locus_tag="PW109_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2510058.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44361..44912)
                     /locus_tag="PW109_000176"
     CDS             complement(44361..44912)
                     /locus_tag="PW109_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2510059.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45017..45547
                     /locus_tag="PW109_000177"
     CDS             45017..45547
                     /locus_tag="PW109_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2510060.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46030..46473)
                     /locus_tag="PW109_000178"
     CDS             complement(46030..46473)
                     /locus_tag="PW109_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510061.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46489..47649)
                     /locus_tag="PW109_000179"
     CDS             complement(46489..47649)
                     /locus_tag="PW109_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2510062.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48156..49856
                     /locus_tag="PW109_000180"
     CDS             48156..49856
                     /locus_tag="PW109_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510063.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(49958..50791)
                     /locus_tag="PW109_000181"
     CDS             complement(49958..50791)
                     /locus_tag="PW109_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2510064.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51095..53260)
                     /locus_tag="PW109_000182"
     CDS             complement(51095..53260)
                     /locus_tag="PW109_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2510065.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53520..55814
                     /locus_tag="PW109_000183"
     CDS             53520..55814
                     /locus_tag="PW109_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2510066.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55807..56472
                     /gene="pgmB"
                     /locus_tag="PW109_000184"
     CDS             55807..56472
                     /gene="pgmB"
                     /locus_tag="PW109_000184"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2510067.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56465..57472
                     /locus_tag="PW109_000185"
     CDS             56465..57472
                     /locus_tag="PW109_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2510068.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57494..58513
                     /locus_tag="PW109_000186"
     CDS             57494..58513
                     /locus_tag="PW109_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2510069.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58794..60161)
                     /gene="nhaC"
                     /locus_tag="PW109_000187"
     CDS             complement(58794..60161)
                     /gene="nhaC"
                     /locus_tag="PW109_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2510070.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60270..61691)
                     /locus_tag="PW109_000188"
     CDS             complement(60270..61691)
                     /locus_tag="PW109_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2510071.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61934..63811)
                     /gene="tdc"
                     /locus_tag="PW109_000189"
     CDS             complement(61934..63811)
                     /gene="tdc"
                     /locus_tag="PW109_000189"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2510072.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64102..65358)
                     /locus_tag="PW109_000190"
     CDS             complement(64102..65358)
                     /locus_tag="PW109_000190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2510073.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65470..65675)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            65938..67239
                     /locus_tag="PW109_000191"
     CDS             65938..67239
                     /locus_tag="PW109_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2510074.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67308..67907)
                     /locus_tag="PW109_000192"
     CDS             complement(67308..67907)
                     /locus_tag="PW109_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2510075.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20370 a        13778 c        12096 g        21682 t
ORIGIN      
        1 tttccgaata aactgtgttc cagaaatagc ttcttcgaaa ataagctgaa atttcacaaa
       61 aatttgagaa gcaattttcg tacattcctt cttctttctt gaaactgacc acttctgtca
      121 caccctctct tctatcactt tccttttttc acacgactcc cggtttcttt gataaatctc
      181 gatactttca attttcttga atttttcatt tgaggtacac atagatacag acgatttctt
      241 gctctagtca tcgcaacata aaatagtctt cgttcttctt ctatcgtcgt tactggttct
      301 gcttttattt cattaaagtc gattactttc ttttctgtcg ctccgttata aggcaaactt
      361 ccttcgttac aaccaatcaa aaagacattt cggtaactaa gacctttact tttgtgaatg
      421 ctctggatac atagtgcatc aagttttcct tgtctgtaag caatcaagcg ggtttttaat
      481 tctttagctg tcgcttgggt ttcttgcgat aaggcttgga gtcttctgaa agtagcatgc
      541 tgctttactt cttctcgtat ccattcttga tcactctggt cgatttcctt agctatacgt
      601 ggcagatgaa gatagctttg aacaagcgaa tagatttctt gatgctggtc tgttttacgc
      661 aacgcttcct caaactctcg gatttctgca gatttcgatt ttaaattggg gcttatcgac
      721 aaaatataat ctgcaacggc tatattttga tatttaggag aaaagtattg atcgcattca
      781 ctttttttca ggtagcagtc tgccatcagc tgccggagca attgctggta ggcctgattt
      841 ttcttttgca gattcagtgc tgtttgccaa tgaacaattt tttgatagaa atgttggaaa
      901 gacttgatac caaagatacc ttcatatgga ctatctgctt ccagtacaaa aggaatattc
      961 gttttcagca gagattggac aagctgctgc gtttgtgaag ctgatcggta taatatgatt
     1021 gattccttat atttcccatt tttttgaata aatgtctgaa tttgtttgat tagccactga
     1081 ctttctgctt tagtatcaga aaacaaatag gcttccataa cacctgcttc tttttgtgct
     1141 ggaatcatcg cctttggtat ccgatcattt ttattatagc tgatcaatct atttgctcct
     1201 tggacaatag ctgcccgaga acgataatta gttaataaga aaagagtttt ggtaggatat
     1261 tctttttcaa agttcttgat gtatgctggc tctgatcctc tgaatccata gatactctga
     1321 tcatcatctc caacaacaat caacttgatc ctgttttcct ttgtcatccg tttcacgatt
     1381 tcccattgaa gtggattagt atcttgaaat tcatcaatga atagataatc atatttttgg
     1441 ccgatataat gctggatctc ctcattctcc aaagcgcttt tcagattttg gagtacatca
     1501 ttgaactcta gcaattgctg gcttcttttt tgttcttgga gttccgtgag caatccatac
     1561 agtcgttctg atatcggctg tcgtgcattc ttatcaatgg aatcatattt ttctttaact
     1621 aactgaacca tttcttctac tgaaaggttc atattctgcc aatagtctaa ccgagaagca
     1681 atcgtttttt ctccatattg atcaaatata taaagattat atttacgtat cagctgttgc
     1741 aaaacagcat tcactttttg tgcagtatcc tttgaaaagt ctccttgtat tccttgcatt
     1801 gctacttcag gaaattttcg acaaatttca ttgtatatcc taaaaaagat actatggaat
     1861 gttccgatat ggacagcggt ttttctctgg ctgaaattat ctactcgttt ttttagttca
     1921 tttgcagcgt tccttgtaaa agtgatcatt agtatgcgtt cttcgtctac ttgtttttgc
     1981 gtcatcagat aaacagcttt tgcacagatt gtttgcgttt tgccgctccc agctcctgct
     2041 atgattcgaa gatgtttctt catatcatat gtgatagctg ctttttgttc ttgattgaag
     2101 cgattatagg aacgtttctc catcatttta aagaaagagc tgtttccttc actttctttt
     2161 acttgtactt cttcctcaat aagtccgggt gcttcgccat aagccgcttc ataggtagca
     2221 atgatttctt cttgtagccg aaagacttgt tgaagctttt tatacttgtt ctcccagcgt
     2281 ttcaattctt tttgtaatcg gagaatttct ttttcattca actgattgat ttccttccca
     2341 tctttcttct ttgaatttgt ttatttcaag ttggttattt aaagtttcgt tgtctatttt
     2401 ttctttatac taacagacct ttctaaaaat gaaaatggga agcgatatga ttttagtttt
     2461 ctgaaaattt ttcctttttt tcaatcgttt atgctaagat aaggaagaca aaaatattca
     2521 taaaaggtga tgaaaaaaat gcagacttcc tttcacgagg ggcgtacaat aggacttttg
     2581 ctgacattta tcggaggagc catggacagt tatacttata tccaatatgg tgcttttgcc
     2641 tctgctcaaa ctggcaatat cattctatcg atcatccaag catttgacgg agaatggcta
     2701 agtgttggta aaaaagtatt gtctaccctt ttctttttct tagggattct tctaacaaag
     2761 tatctaattg attattttag aaaaaaagaa aaacatttct ggcgcttgtt tgttctatat
     2821 tatgaagcag tgattttttt ctttgttagt ttgacacccg tccatgagca tcccgcatta
     2881 gtaacgattt tgattgcttt tactgcatcg atccagtggg tagcttttga taaaatcaac
     2941 gggcgtgcct acactaattt atttacaact ggaaatttaa aaggggtagc aacgaactta
     3001 tatgattatt gtatttctaa agaaaaagca gattttgatc gatttttcca ctatctaagt
     3061 gtcgttcttg cctttatcgc aggagctatt gtccttgttt tctgtcatca tctgtttggc
     3121 gcccattcga ttttgatcgt ttccatttta tttttgatca tggccctcac tcagacattt
     3181 catttatggc aattttatca tacgaatcgg ttcactattt gatctcaatg aatcaaaaaa
     3241 actaagcaag atgctgctca cagagaaata tctgcgagtg agcaatttgc ttagttttta
     3301 tattatttag ttgtaaagat tccctttgct ttcgggttat ttgtagtgta gcggatcgac
     3361 tgggcatctg cctcttctgt cattttcttt gcccgcatct tgatttcttg tacgatttgt
     3421 tctccacatg ccggatcggc tacgaaatca tatttgtccc catcgatcac tattttggga
     3481 catatatcaa actgttcata ccactcttcg tatcgtcgat tgagttcttt atagtaggaa
     3541 tagagttcag gatcttgttc tagctgttca tattcccggc ctcttttttt gattctcgct
     3601 aacatagttt caaaggatac acgaatataa accagtagat ctggtttctt ttgtggcgaa
     3661 atttcttcta actccttcag catcgtttct aaaagacctt catactgctt tacttctact
     3721 tcagaaacac gccctaaatc tgcattcaaa tgaaacagca tactatcttc gtagatggag
     3781 cggtctagta cgttgtcatc atggctgaaa gcatctttga ttgccaaaaa gcgtttgttc
     3841 aaaaagaaaa tctgtaataa aaaagtatac ttttctggat tagaataaaa tagcggcaat
     3901 acttcattat cttccacgtt ctcataaaaa ggttttgttt ctaaatcttg ggcaagcatt
     3961 tcagttagtg tgcttttccc tgcaccgatc gttcctgcaa caatgatcac aattatcggc
     4021 atccttcctg taataaaatc tgttcttcat tttactactt catttataga aataaaagcc
     4081 ttgactttat ctgctcctta aaagctactt ctctaaaagt agccaaaagt aactcctatt
     4141 catgaaaata aaggctgtga caaaagtctt gtcgcagccc ttttttcaaa taaacagtat
     4201 tctcctgcca acacctcggt cacaaggcaa tattcataaa acatgaaaag ctgtttttta
     4261 acattattcc ttgtaactca gagctaaacg ggagactcac aacctcttta tgctctcaac
     4321 attttatctg tttttattct tctattgtac ttgatttgcg tacaactaac tgccagtgca
     4381 gattatgagc ggtatcattg tcttctactg cgcatttcat gacacgtatc atcacactat
     4441 tttcatagat tttttctatt tttcctttta ttggtgattc aaataaatca gatttaaatg
     4501 catatgtttc gccgacttca aacaattgtt cattttccat aagaatccct ccatcacctt
     4561 ttataaggtc agtaattttt taacataaat cactcgaaaa agcacaaact tgtcataaaa
     4621 agtacttttc gtatcaaaac tgtcactttt tttggtaagg ttcttggaaa acgaagattt
     4681 ttttaggaga tcatgacggt tttgatgtca aaaccacttt ttatgaaaca aaagacgttt
     4741 tttatttttt ttagcataaa atttgtggtg gaggtacgta tgagatgaaa gataaagttg
     4801 tgaacaaatt attagaagcc ttattcagcg gagacaaatc ggaagaagat atggtgtatg
     4861 tccaagtaaa atttgtctta gaagttttat tgaataatct tggaaaacta gcggtcgttc
     4921 tagtcttttc tctggtgaca ggctcatggg cggaaacagg aatcaccttt ctttcttata
     4981 tctgcatccg cagatatgct tatggattgc attccgactc tgaattcgtt tgtctgctgt
     5041 ggacactact ttatttatgg ggcgttccct tggtgatgaa acatctgcaa ttgactatct
     5101 ccttcccaat gatggtcttt ttgttaatca gctgcttttt gttactgctt cgctatggat
     5161 caagaggtac agcaatcaat cccattgaac ccgagaaacg tcccccatta ttaaagaagg
     5221 cgatcagcat gtttctaatt ttttctctca ttacgttatt tttctcagct tcttactttt
     5281 ctacctattt gctacttggg attgttttag aaatagctac gttgcttcct attacaaact
     5341 acttgatgaa ttttggagga atctttcatg aaaaaaacaa tcattaaaca attaactaac
     5401 ttatctgact tttttgtatc tggctgctgc tttggtatga gttctgaatt caaaatccct
     5461 gaaaaattaa aaaaataata cgccaattgt gctataattt ggtaaaaatc aatgggagct
     5521 gtactttatg tttttatggt ccatagcgtt gcaatatttg atttacacaa tttgtatctt
     5581 attgattgac catcacatcc gatacaaata tctgtttgcg ggagtcattc tgatttcttg
     5641 tattttgatt ggatgggtct attcgatttt agggattttt actgctctaa ttgctatagg
     5701 aatgctgatg cttacttttt tcttcttttc gacttcgcgt caacaattgg tttacgcgct
     5761 tccttttggg ttattgacgt cgcttttagg agatcactta acaagcatcg gcgatttttt
     5821 tgttttgaaa tcttcggtta ttgaaccagg agattcattg cagtattttc acattgtctt
     5881 atccgctttt ttatctgctt tattagcttt ttgtttaaaa aaatcgttag agacttggtt
     5941 tcacactgtg gaccgccgta tgatcggtac gatcgggaca ttgatattat ttacatacta
     6001 tatcattatt ttttataccc gtttttctgg tgaaacacct gatgtcttag ctttgaatac
     6061 gagttttttc attctatatc tgtgtgttgg cctgatcatc ttggtttatt attggaaaat
     6121 ctctaataag aaattagctg atcagttatt agagcaacaa gtcaggatgc aacaggatta
     6181 tatcaaagat ctagaaaaaa actatcaaga cttgagagaa ttcaaacatg attaccaaaa
     6241 tctgcttttt tctcttcaca gctatatttc agaaggcgac ttagaaggat tacggtctta
     6301 ttatgatcaa acgatattac ctacacgcca gatgatgaac ctttttccgg ccaatctaag
     6361 tatgttggat aatatgaaag ttcctgagat ccgtagttta ctgtcattaa aactcatgat
     6421 ggcacaggaa aaagggttga tcgtccagct tcattttcct gagccaatcg aactggatac
     6481 gaaacatacg gtaaaccttg tacgtatgct tggtattatc ttggacaatg cgattgaagg
     6541 agctagtgct gcaaagaaga aaaaaatcga attgaccata ctcaagaaga atcattccgt
     6601 gatgattcgt gtgatcaata caacaacaaa tacattaccg ttaaaccagt tgaaacaaaa
     6661 agggttctca acaaaaacaa atccaaaaaa cgaaggcctt ggcttgttta tcttggatga
     6721 attgacaaag accaatcctt atcttttcct tgaaacatca attgaaaacc agcgtttttc
     6781 tcaaacgatt tatatacaaa taaaataaag gagtaatgat atgattccca tttatatttg
     6841 tgaagacgat ccaaaacaat tagaagtttt atcaacggtg atcaaaaata gaatcatgat
     6901 tgaaaactta gacagttatg tccatatttc tacctctgat ccggaagaac ttctcaatgc
     6961 agccaagatc agaacttctt cttttggtat ttacttttta gatatcgaac tagaagacag
     7021 tgaaatggat ggtgtagcga tcggtaaatt gatccgtgaa ttagatcctt taggaaaaat
     7081 aatctatgtg acttcccata cggaaatgtc gatgaaaatc ttaaaaagca acatcgaacc
     7141 gactgattat atcgtcaaag aagatcttta tgatttaaaa gaaaatgtcg agcatatcct
     7201 gagcaaaatt ttcgaagatt ttcaaatgtc ccaaatccaa aaagatatct tcaaaataga
     7261 gtttaatgat gagatcaaat ttttaccgat caaagacatc caatattttt ctacttcgct
     7321 aggagcgcca cataaactgg aagtccattt gactcatgcc caattacaat tctatgagaa
     7381 gatcaaagaa atcgagaaaa tgcatcggaa ttttatccgt tgtcataaat cctatgttat
     7441 caatacacaa aatgtgcggt cgattaataa aaaaaccaga gaagtcacgt tggcaaacgg
     7501 cgagaccatc ccagcctcta tccgtggatt aaagaaattg atttcataaa aagaaccgaa
     7561 tagtttggac cgactaaatg accagccgat ccaaaccctt cggttctttt ttgcctatca
     7621 gtgcaaaagc atttcctgtg caatttcacg tccatccatt gtatctggat aaaatgttgg
     7681 ccaattctcc atttctttca gcaatacttc ttgactttca tttcctgcat ataaatgaaa
     7741 atgttcggag tcagccggtg caatcgtgtg atcactgaat tgaatatatc taaaagcgcc
     7801 actttcctta tcttctgctt cgaatagata ccttacgcca cggtttcctg cctcgtaggt
     7861 caagatttct ttccctacat aacgataatt tgaacgtttc gttgttccat tcttaaacgt
     7921 aaatgccatc tctttctcac tgatcttgat tgaagcaata tctgtttgat agcccttcgt
     7981 ataatattct ttatattctt gtgctgtctt atcctgattc aatttttctt tatattcaaa
     8041 aacaggatca aaagtcccat cttctacata cgtatctact gtttgccaag ttcctgccca
     8101 atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa taaccgttgt agacgctctt
     8161 ttcttttttg ggggtcaaag tatcttctag cgggttttct gtctctgtcg tttttttcaa
     8221 cgcttcgagg tttgcttcca tgatagaaag atagttttct ccattttcta tttgttcatt
     8281 cgtcaatcct tccaaaggat tcaatacttc taaagataca cctgcttcgt ttgctaacgt
     8341 tctggcaata ctatctttgg cgttttcttc aaaataaata tagttgattc catgttcttt
     8401 aacaaagtgt ttcagttcag caattctcgc cgcagacggt tcttcactag aagaaagacc
     8461 agcaatcgat acttgattca atccatattc taacgctaga tagttgaatg cagcatgttg
     8521 cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct tgataaagct gatggagtgc
     8581 ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat acttcctgtt tttctgggta
     8641 agcttcaatc agctgatcac gtatgtttgc tacctgcttg attgctaaac ttggagctag
     8701 ccatacatgt gggtctagct catgtgtatg gccttcttcc ccgtgttcat ggtcatgatc
     8761 atgtccttct tcacttcccg gaagtaaaag cataccttct gtcgctttta tcgtatggac
     8821 attgccttct tgtaatgttt tttggattgc ggggacccat gtttccatgt tttcatcatt
     8881 ataaacaaag atatctgtat cttggatctt tgccatgtct ttagcagaag gttcataatc
     8941 gtgggaatca gtccctgctg gaatcaataa ctctacttct ccttcatccc cgacgattgc
     9001 ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg ctcaattttt ctttactgtc
     9061 ctcggctttc ccacttgccc cgcaagcagt taacatacct gctaaaacca gcatggccaa
     9121 tatacctatc aatcgtttca tttatgacac tcctcttctc atcttctttc atttgaatgt
     9181 tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag caacaggatt taatttatca
     9241 tatggttaaa agaactgtca agcgttttct tattttaatt gatgagaaag tttatatgaa
     9301 tgattgacga tatttttgca atcaacctct atcattttcc ttttataccc gcttttgtct
     9361 ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa agaaacagat acaacgaatc
     9421 gtttgatccg ctttttagga ggaaaaacct cttactatgt tttaggaata gttatcttac
     9481 ttgcgctgat catttatttt ttccatcaga tctcgtttat attcaagcct gtcggaatca
     9541 ttatcgctac tatcttgccc ccattattgt ttgcactgat cttgtattat cttttggaac
     9601 cagtcatcaa acgtttgagc aagcaccttt cgaggaactt gtcagtcatt atcgtttatg
     9661 tgattatttt atttttattg gggcttggaa gcatatggct tattcctttt cttgaagaac
     9721 aggcaaagac attgatcagt agtttgccag atttgtttca agattttcaa gagagtctcc
     9781 gacaattttt ggagaaaaca ccttttgccg attcatttaa tcaatttttc gcttctattg
     9841 acgacgtgac gtctgatata gctggattta ttggaaatta ttgggaaagt ggcgcacaac
     9901 ggatcagcaa cattttttca actgttacag ctatttttat tactttattt actggtccaa
     9961 ttgtcgcttt tttccttttg agagatcctc aaaaatttta tcagtcagtt ttagcgattg
    10021 tcccaccagc atttcgtaca gattttaaaa atttgacaaa gattgctaat caacaattgg
    10081 gttcatttct aaaggggcaa atcattgctt catttatcct tggagcagtc tactgggttt
    10141 gtttcttact gatcggatta gaatttgcta gtgttttagc tatttctgct gggttgttat
    10201 gtatcattcc atatattggg ccgtttatcg ccttctttcc aggtttgatc attgcattcc
    10261 aagactctac ttttatggct gtcaaatttg tcattgtctg gtttgccgta caacttctcc
    10321 acggtgactt ggtgattcct cgagtaatgg gtgatcgttt acagatccat ccgattacga
    10381 tcttaatcgt tttacttgtc atgggtgatc taatgggaat tgtgggcgtt atttttggta
    10441 ttcctatcta tacgttagtc aaattactcg ttacttttat tttccgtaaa ttcaaacaac
    10501 gctacaataa attttatggc gataaaggag agtatgaaaa ctcttcattt tctgaagaag
    10561 attattttaa ataaaacaca aaacgtcgat cttacgagaa tagctcgtag gaacgacgtt
    10621 ttgtgttttt gttttaattt ttcagcaaag caattgcctg gtatggtttt agctccagac
    10681 tttgatgaag aacagtatcg gaataattat cgataagaat attggcttct gtgaatcctt
    10741 ctgggatggc gatccttgta tctttatcat agaaatttgt taatacaagc agctgctgtc
    10801 cttcaaattg gcgaagaaag ccataaactt ccggatgatt tgcttcataa gcatgataat
    10861 ctccttgtgc aacgattgga tatgtttttc tcagccgaat caatttcttg taataggaca
    10921 aaatagaatc ttcagaagat agttcttttt ctacattaat ctcttctagc ttgcctccaa
    10981 cttttaacca aggtttccct gtagtaaatc ctgcttgttc tgtgtctgac cattgcatgg
    11041 gtgttcgaga gttatcgcga gattttgctt tgatccgacg aaacgcttct ttttctgtga
    11101 atccttgttc aaggagtaac ttataagcat tcaaactttc tacgtcaata tagtcggaaa
    11161 tcgatgaata gtctgggtcg atcatgccga tttcttctcc catataaata tagggcgtcc
    11221 cacggttcaa atggatcatg gttgctagca tctctgctcc ttgttttcga tactgatcga
    11281 tagctacaaa tcgattgagc gcacgtggct gatcatgatt attaaaaaat agagcattcc
    11341 agccgttacc ctcactcatc ttttctcccc atgtatgaaa tagtcgcttt aattctgcaa
    11401 aatcaaatgg aggagtagac catttattcc cattttcata atcgactttc aaatgatgga
    11461 aattaaagac cattgataat tcttctctac taggttctgt atacaagata caattttcga
    11521 tcgtcgtaga gctcatttca ccgactgtga tgatagtgga atccgtccca aacgttgctt
    11581 ggttcaattc ttttagatac gtatgggtga ttggtttgtc cgtatattca ggtttgccat
    11641 cattttcttt attatttttc aattgttcgt ctttaccgat aacattgata acatcgaaac
    11701 ggaatccttt tacccctttt tccatccaaa aacgaacaac tttgaatagt tcctctctta
    11761 ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc atataagtgc aaataatatt
    11821 tccccgtatc tccaaatggc gaccaagcac ttccaccaaa ttttgatacc caatccgttg
    11881 gctcttctct taaaatgaag aagtcctgat aatgctggtc ccctgctagt gcttttttga
    11941 accattcatg ttctgtcgaa acgtgattca gcaccatatc gagcatgatt tctatccggt
    12001 atttctttgc ttcggctatt agctgttcga attcctccat cgtaccaaag ataggatcga
    12061 tggctgtgta atcagagata tcataaccgt tgtctttttg agggcttgga taaatagggt
    12121 ttagccaaat catgtcgatg cctagatcat tcagatacgg gagtttttct ttgattcctt
    12181 ttaaatctcc aattccatca ccattactgt ctttgtaaga tttgggatag atttggtaaa
    12241 tgactttgtc tttaaatgtc atggatattt acctccattc aactgattta ttacctacca
    12301 caacttatat atacaagtta tcatttatta ttataactga accgaaagcg tttgtaaaat
    12361 ttatctttat acattctaag aggtctgttg cccaataaaa aacaagagat cagacatgta
    12421 tttgtctatc ctcttgttta aaatagcaaa atattcttgt ttcttctata aatggtgttc
    12481 aaaagctttt atctacggca atcagtcttc tcaaatgtaa aacatgagga gctaatttta
    12541 tttgagtatt ctttttgctc gatgcaggac ggctttttta caacctctga ttcatggtgt
    12601 tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgaaaa actattttgc
    12661 cgtttgtttc tctaatactc aggtcaaacc gctgatttca caacctctgt ttttattcat
    12721 tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta acaatcatca
    12781 caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac catgtgatca
    12841 ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca gtcaatatat
    12901 atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt tctcgaacaa
    12961 tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat aattcgcgta
    13021 cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc agcagtcctg
    13081 ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct tcttgcgttt
    13141 cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc agtttctcgg
    13201 aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat gcattatcca
    13261 tcatcgattg atcagtaact gaagcataat attcagggga catataaata aagctaccta
    13321 gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct tcatcataaa
    13381 gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc gtaatagcga
    13441 ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg ataaaacgat
    13501 ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca tgtccttttt
    13561 ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt tcttgcttct
    13621 gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat gtaacgattg
    13681 cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga gaatctttca
    13741 atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc gccataagca
    13801 ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag ctaaggcgtg
    13861 accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt gctttcggtt
    13921 gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt gcagcgccaa
    13981 atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca taaaaaataa
    14041 gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt ggcaaaagat
    14101 tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt gcatagattg
    14161 catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc ccgatttccc
    14221 tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg gcaataaaga
    14281 aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga tcaccaaatt
    14341 cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta gttaaatttt
    14401 caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca cgttctttct
    14461 caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat gtttgaacag
    14521 cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc gctaactgtt
    14581 ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca tccgctattt
    14641 tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct aagcgttctt
    14701 cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt ttatctttgt
    14761 aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat aagacagaat
    14821 aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg ctaccatttc
    14881 cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg acaaagcccg
    14941 tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata tcaccgattt
    15001 tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt tccgggagtt
    15061 ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa aatatttgaa
    15121 tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct acttttgcat
    15181 ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta gaaacgattt
    15241 gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc atgacaggtc
    15301 cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg atgattgcaa
    15361 tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat gttcttgttt
    15421 tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca tttaattcaa
    15481 cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc gctaaagcag
    15541 aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt tgcaataatt
    15601 tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca cataaaagaa
    15661 gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg cctcctgata
    15721 attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat acctctacag
    15781 gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt gctgttagat
    15841 taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg taagcagtaa
    15901 gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga ccttcatcag
    15961 gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca cttggaccaa
    16021 gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt gctgtaatgg
    16081 tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac ttcactctga
    16141 ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt ttctcacact
    16201 tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa tttagttgac
    16261 aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag aaggaacatc
    16321 atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt tgtcgcgatc
    16381 gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc agcatctatt
    16441 ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt gaaagtttta
    16501 gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat gggtggaatg
    16561 actgcttctg tacctccaga attcactgat ttacttttga aggattcttt gaaagataca
    16621 gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac ttctcttgat
    16681 cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc gacaacaaac
    16741 ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt gcttgctgaa
    16801 gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa gatatttccg
    16861 aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca aagcaaataa
    16921 gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa taaccacagg
    16981 atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa gtcgctaaac
    17041 tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg gagaaattca
    17101 gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat cagcctgact
    17161 attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc gttcctgata
    17221 tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg tataagcaga
    17281 atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc gaatatctag
    17341 ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct gtctctacag
    17401 atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg gatcagaaaa
    17461 aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt tacctagcgg
    17521 gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct gaaaatccac
    17581 cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt taccaaatcc
    17641 ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc taccagatca
    17701 cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat gaattagctt
    17761 ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac tacagcatta
    17821 ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca acgatcgcac
    17881 aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg atccaattcc
    17941 cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga ttttcaactg
    18001 cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa aacgttttac
    18061 taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg aggtttgtaa
    18121 aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc gtttaaccaa
    18181 catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg gcggaggtaa
    18241 aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt ttattggagg
    18301 cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag aaaacatcga
    18361 tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga ttatggtaga
    18421 cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt tccatgaaca
    18481 agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg ctcagtttga
    18541 aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag gaaaaacaac
    18601 gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg aaaagacaga
    18661 tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag tgacagatca
    18721 taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa cagtaatcat
    18781 tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta tcgtccctgc
    18841 ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg gcgcattaag
    18901 cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg gaaatctagc
    18961 ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc gcgaagcttt
    19021 agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga aaaaaggaaa
    19081 agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg ataaattaag
    19141 tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt tggctgtctc
    19201 aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac ttgaggtaca
    19261 atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg aaaaaatcaa
    19321 attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga aaaaagagtt
    19381 gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca atattatatt
    19441 agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat ggtactgtcc
    19501 gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga ttctgtcctt
    19561 ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa tgcagaaaaa
    19621 ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata tgcagaaata
    19681 atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat cgatcattca
    19741 gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc ggaaatcttt
    19801 acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt tgttttagcg
    19861 actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc tgctgcgtcg
    19921 aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa aacgtttaaa
    19981 gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa tctgacctct
    20041 ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt agtactgatg
    20101 cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc ttttgttgaa
    20161 aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc tttactttca
    20221 ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt ttaccttggc
    20281 gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat cagtggcgca
    20341 atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc tgtaaaccct
    20401 gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt gacaggagca
    20461 gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa acgttcaaaa
    20521 acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt gaccgaacca
    20581 actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg cattggtgca
    20641 gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt tgtatttggt
    20701 agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt agcaggactg
    20761 gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga tgaagaccta
    20821 atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca catatttcag
    20881 tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc aaactttggt
    20941 ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa caaaggtggt
    21001 ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt atttacaaat
    21061 tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg acacgattat
    21121 ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga aaggaaaagg
    21181 actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact tggctgattg
    21241 ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata gctgatcaaa
    21301 cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg gattctttta
    21361 cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca actgaaattg
    21421 atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt acaattgctc
    21481 cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct gaacagcaac
    21541 ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg aaggtaactt
    21601 acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat ggtgccaaag
    21661 aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct attactgcag
    21721 aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt gaagaagtcc
    21781 ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac aacaataata
    21841 atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat tcttcgaatc
    21901 aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca aaccatccaa
    21961 acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat acagcaaatc
    22021 aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa tcaccaattg
    22081 atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga gaagcattca
    22141 agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt gactgttctg
    22201 gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc tggacaggcg
    22261 aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc gacctcgtct
    22321 tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg aacggacaat
    22381 ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca gatttcacgc
    22441 ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt tcgctgacag
    22501 gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa aaccaaacaa
    22561 ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa aaagaaggaa
    22621 tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga aacagcttat
    22681 tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa ggaagcagcg
    22741 tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc cgcgcacaat
    22801 tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg atcaaaaatg
    22861 gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca aaagactaca
    22921 aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat tcacaaaaat
    22981 tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa aaagaagcag
    23041 caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc gatgtgccaa
    23101 tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg ataaaacgcg
    23161 tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg aacaatttta
    23221 cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact gttcctgtac
    23281 taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag aaatagaata
    23341 aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta tttctttata
    23401 tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt gacatctcca
    23461 aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat accgccatga
    23521 tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc tgtaaaaatc
    23581 acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg gcttgtcaat
    23641 tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc gatgccgccc
    23701 actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt aggacgttgg
    23761 aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt atctttattg
    23821 agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga ctgtgctgga
    23881 tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt gcggtcactg
    23941 atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg tacttctaca
    24001 tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa aatgaccgat
    24061 gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa gctaggtgca
    24121 ccaccatgta aaccaatgac cagtgtccgg gtctgttttt acttcgaacg tttcatcatc
    24181 taatttgaat gtgccattcg taatccgtcc agccatacgt ccgattgaag caccgatata
    24241 agggtctttt tctagatatt cttttgcttc atcaaaacct aagatcaact ctcgtttttc
    24301 attatcgatt attgtttgaa aatgaacaac ccgtgcccca agatcactga cagacaaaga
    24361 tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt tcgccaaaat tttgttcatt
    24421 gatttccatg ttccttgcac cctcttttct ttcatttgca ctgattccag catacatgga
    24481 aaccgttttc ttaacagctt attccgtgag taacgagaaa aaatttacta tattctaata
    24541 aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac aacctctttt tccgaacaaa
    24601 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgaggagc
    24661 tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca atctcttttt
    24721 caatacgatt ccagtatata accatgtcgt cagtacatag tagatgaaat acaatacgaa
    24781 gaatatcgta aacggaaccc aaatatcttt gtatggattt tgcatgaata gaccaaacat
    24841 ttgtaatccg aataagacat ggactacacc taagattcct ggtgctaaga ataacacgcc
    24901 tacttctcta cggatcgatt gtttaagcaa cccgcgtcgt gttcctatct tttcaagcat
    24961 ttcataacga gcaatgtcac tcttgcttcc tgacaagatc ttgaacatca aacagctagc
    25021 aagcatggtc aagaaagcta aacctaggaa gaatcccata aattcaaaac cagagaatag
    25081 accattatac acttcataca tttcatattt ctggttgctg tactctactt tttgcaaaga
    25141 tggattgttc attttatttt gagcaacaag tgtttcgatt ggtttcaggt ttgcttggaa
    25201 gtcttttacc tgaactagct gtaaagtgga ttgtggcaag ttcagttgat caaattctga
    25261 ttgactgact accttgattt ctttttcttg ctgttccggc aattcatagc ttcgaagttc
    25321 ctcttgtaca gtcagttcat tcattttttt agcaggaact ttttctttcg tgatcttcgg
    25381 cggtgttcca tcttcatgtt ttatcatgat caatggttga gccgcaaatt cacttgcatt
    25441 atagtaaatc gttcccgcat cttctttttg agtataagaa gcagaaagtg ttggtgaaag
    25501 ttctttgatt ttttgctgat cttctgcccg aggattattc atcactagat catatccaga
    25561 attgttttct gtcaagtctg caatttcatt tttaaaacct aatcctaccg tcaaagcgcc
    25621 cagtgccaag gcaaacagca tagcgaccat tgagagcatt tgtgtatatt cacgaatacg
    25681 aaagtttaac tgtgacaatg tgaaattgtt caattttttc aatgaaatct gttctgattt
    25741 ctttagtaat gataaaaaga aaatgatcac agaatggaaa atgaaataag ttcctaatac
    25801 gatcgtaact aaggctaccc ctataccaaa gacttgatag gtccccacgt gtttcatcat
    25861 atagtaaccg cagcctagaa aagcaattcc cagtactact tcaaggaaaa acaagaattt
    25921 atgctgcttg attcttgttg gtgtctgatc cgcacgaatc aatgctaaaa tcggtttttt
    25981 gacaatcgaa aaagcattga caacagcagc taataaaaac aaaacagcaa aaaacaaaac
    26041 agtgatcaac aacccgtgaa tactaaaagg tgtaaaatgc gaaatattga gatctaactg
    26101 atccactaat aatcggtgga cgatacttgt caatcctaca ccgacagctg aacccactaa
    26161 agtagctgcg atccctacta agaatgtttc agaaaaaatc atctgtgcga tttttctgcc
    26221 ttttgctccc agcatcataa acattgcata gtctttttgc cgcatcgtca tcaaaaagga
    26281 atttgcatat aaaatataaa cgaatgtgat gatccctagt aaaacagaac cgaatcggaa
    26341 aatgataact gtagccgcaa cgatcgtatt acttttcaaa aaagcttcgt tgcttgccat
    26401 tgattcaaac atgtaaaaaa tagcagatgc cataactaat cctgagaata acaccaagta
    26461 gtctcttaaa cggctcttga tcccagtaag tgataatttc caaagcatag tcaatctccc
    26521 cctactgttc caagtttcca agaatagata gtatttctcg atagaaggaa tcacggcttt
    26581 gatctccacg tttcacttct tgatggatac cgccatcctt gatgaataaa atacgttggc
    26641 aataacttgc agaaaacgga tcatgcgtca caagaagaat cgatacatga tcttttgtat
    26701 tcaattcgtc catcgtatct aaaagatccc gggcactttt ggaatccagc gcacctgtag
    26761 gctcatcccc taatagaatc gttggttgtg tgatcaatgc acgtgcggcg gcgacccgtt
    26821 gtttttgtcc accggaaatc tcagaaggat agctttccaa aatatgagag atggataaac
    26881 gttctgcaat tttttggaca cgttgcttga tttcttttgg tctgacgcct tgtaaagaaa
    26941 gaggaacagc gatattttct tgtgctgtca agctttctaa taaattgaaa tcttggaaaa
    27001 taaagccaat ctcattcgca cgaaaatctg ctagttgatt tccttttaat gtggtcacat
    27061 ctttttgatt gattcggata tgcccatctg ttggtttatc taatgtggat aaaatattca
    27121 aaagtgtcga cttaccagaa ccagaagcgc ccatgatccc aataaattcg cctttttcta
    27181 catcgaagct gatgccgctt aatgcttgcg tctttttctc attgtttttt ccgtatactt
    27241 ttgttacatt ctgtacttcg actattttgt tcatttcttt ccctaccctt taattagttt
    27301 ttccagtgct gttttcggca cgtctttttt ttcgacttct tcatacgaag tgacaccttt
    27361 atttttgttc caggtgattt ttaaatatgc tcctttacga agcggtcgag tcaattgtcc
    27421 cttgaagtcc agttcttttt ccttaccctc tttcgtatag ccaggcagga tatatctata
    27481 gttgactgtt tcttgtccag agtctgattt tgaaactgtt tttgtaccat ctgtcgtaat
    27541 ttgtacatag tattcttctc cacccataac tacgtaatct gcgattttca cccctgcaag
    27601 taccacaacg atgaaaacaa gtagaccgat cagaagtttt ttcacttttt ccccttctct
    27661 cttactttat atactgatta tagaaaaaag cgaacaattc tgccttcgaa tcaactatca
    27721 ttcttctttc aattttgtca ttttctttaa aacaactaaa aaaagagttc aagacaataa
    27781 tccgtcctaa actccttctt cctatcttac aaaaagtatg tcacttacgg tctttcatgt
    27841 cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat gattgagaca agtaaattat
    27901 tgatcaattg gactttccga tcatcggtga aattgatttc ttgtccatct tcgatttgct
    27961 ctaaggccat ttgagtgatt gtgacagctc cttcaacaat ggtttgtcga gcagacaaaa
    28021 tggcttttgc ttgttgacgt tggagcatgg cactagcgat ttctgttgca taggctaaat
    28081 ggttcaagcg tgtttcgatt acttcgacac ctgctactgc taaacgctct tgcagctctt
    28141 tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag cgtcaaatca ttctcattga
    28201 atgtatcata cgggtattgg ctagcgatat gccgaatggc tgtctcactt tggatttcta
    28261 caaaatcttg ataatacgct acatcgaata atgcctttgc tgtatcaacg actttaaaga
    28321 ctactactgc tgagatttcg atggggtttc catccaaatc attgactttt aaaacagaac
    28381 tattgaaatt ccgaactttc aacgaaacag tcattttttg tgccaaaggt atcgtaagga
    28441 aaaagccatt ttctcgaatc gtacctaaat attgtccaaa aaacaaaatt acttttgctt
    28501 gattaggact gactacagtt gccgaactta acaacaataa cgtgatcaac caaagaaaaa
    28561 tacttaaaaa aagtgcccaa actttcgctt gccacatacc taaatagaag aaaaacaatc
    28621 caactaaagc taatacgacc aatccgacaa tccctgcata accattgata taaaatgctc
    28681 ttttttctct catgctactt cctgcgctcc tttatccttc aataaatatc taatgttact
    28741 tttttaattt ttttttcgaa tacgcgccat tacaccttct agttctaact ctttgtcttc
    28801 agtgatgtac caaccgcttg cctgtgtccc tccggctgcc tgcggtaaat aatccaattt
    28861 tgttcgaacc cagtgctttt cttctgtaaa atagtcttct accaagacct ctagatccat
    28921 ccctgctgaa agaggcaaat cttcccgaac agatttaaag ataaatcgtt ccttcttccc
    28981 atcatacttc aatattcctg tatcgataat cggtaaagaa taataatcca gctctttctt
    29041 caccgtcaat aaatgtttga aaacttcatg gtacacttgc tctaattggc gaccttctga
    29101 attgttcggg taattaacca tcactttttc tttgtctgga tcatagaagg tttggcttag
    29161 ttgagtgata taagtatgta atctttccat ttctgttttt aacttttctc tttgaccttc
    29221 ttctaccttc atatactctt tcaccctgtt ttctttttag atattttctt tcttatttta
    29281 ctatagttat gtgcgttaag cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt
    29341 tttcttaaaa tatccagttg agtacaggat caatctgttt cattgtcctc ttgatccgtc
    29401 gtaatattga tcgatgtgtc tttagcagtg atctttagct ttttctgtcc ttgttctaac
    29461 aaagtatttg ttattggctg gtagtctttt tcaatataag attcaccaga cgtttggata
    29521 gtatagccac tgttttccat ctcatgcaaa gataaattgc caccgtttag gatgactttt
    29581 ccttctttgc tgatcaaact gtcttctaga tggaagttgc tgttttcacc tgtgacgatc
    29641 aagttatcca cattggcctc atctaatcga agtgagccta attcatcttt cgcagttatt
    29701 gttcctcctg taaattcatt gaacgatacc cagccatcat gtgtcttagt attcactttt
    29761 ttgattgata cttcgttgat atcaattgca ctattattcc cttcgattgt caattcagat
    29821 aattcatgat ctgcagggac aacaatagtg atttgtgcac ttgtgtcttc taaactaatg
    29881 attgcacctg ttgatttttt gactgcattc acagtcaaca cgccattttt ctcctttatt
    29941 tctggctttt ctgtcttatc ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc
    30001 gaaactgttt gattttctgc atggatgacg attttgtttg catccttgat tttttttgtc
    30061 tcagtaacca tatcgatcaa ccgaggacca ttatcccaag tcagactctt ttgagcgcct
    30121 aatgtataag ttgcacctgc taggaggcca ccagtaagca tcagaccaat accggtaatc
    30181 aaaaaatgtt tcaatctcaa tttatgtgtc ctccttcaaa cgcccgctga atgcagatct
    30241 ctttttgtaa taaaacgtcg tcccatccat ttaacaaagt tcatcatgac ataaaaaagc
    30301 ccttttgttc ctttcagcaa cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga
    30361 ccgacaccga taaagaataa tccactcaga atcgattgtc caaacacact gattcctgag
    30421 atgatcatga ataggccaac acctaagata ctgatacata aaacatagat actaaaaatg
    30481 agcgctagta aagcaaccaa tagtccccca ataaccaata taaaccccag tgctagtgga
    30541 atgaggacag gagaagcaaa taaaccgata atgattaacc aaatcaggcg aatttgtctt
    30601 tttggatttt tcactaactc gttttctgct tgttcttctt ctgaaaagta actgattttt
    30661 aattttaagg caaactgctt tggtgtacca tattcatcaa tgatctgatc taatgttttt
    30721 ccagaatcta aaaaatattc ctcataatac tggatcaaat caaaacgatc gtcttcagga
    30781 atttcttgaa gatgatccac aacttcattc aaatattcat tcatttgtgc tctcctccaa
    30841 taaccggtct aaagaatctc gaaaaacaat ccattcatca ataatttctt ctaaatgacg
    30901 acttcccatt ggtgtcaacg agtaatatcg acggtttctg ccttcaaaag gttcatcata
    30961 ggtctcaaga tatccttctt ttttcaatct tctcaaaacc ggatatagtg tagattcact
    31021 gacagagaga ctttcttgta cgatttttgt taaggcatac ccatacatat cttccttgct
    31081 taatgctgct agcacagtac catcaagaag aacagttgga atctggatgt gcattacgtc
    31141 accttctatt ctcaagtgta atattttatt tcaatctata ttatacaaca tataatatag
    31201 tttcaaaatc agtttgtcaa atacaaaaaa actgactaaa agatttgatc ctttttagtc
    31261 agcaaaaaat ggcttctctt tgatttttat tataacagtg gcataatcca atttgtccac
    31321 atccagctca gtggcatgat aacgatcatt gcaggtatca tgtcagctac tgggaacatt
    31381 ttcacgttga tcattctaaa accggtagcg accatcaaga atccgccgca agctttaaaa
    31441 tctaagatca tgtctggtgt agtcaatgga taaataaatt ttgccaataa gaacaaaata
    31501 taaaaaataa tgaattgggg aattgctatc acagaaacga cataccctaa attacacgca
    31561 aaaatagctg ctgtgaaaaa atcaaggaca gatttagaaa tcaaaatcgt actgtctcct
    31621 gtcatcccag aatctaaact tccatagatc cccgtaccac ttgcacaaaa tagaacaatg
    31681 accgtcacta atgtgctgat aaattcatcg tgggtcatac caagcttttc actagggaag
    31741 attttagaaa taggtacttg catcagcgca gcccccttat ttatcccatt acctaaatga
    31801 acaatcaaac caatcgctgt tcctatcact aaagcaaaaa taacagccgg catatttttc
    31861 atcggcgcaa tggaatagat tcccatcccc atcgaacaca caccaaaaac catcgtcagc
    31921 tgtgctttaa aatgttcact taatttattt cctactaatc cgccaattag tccgccaaac
    31981 aaaacggaac ctacattgat caaaactcca gttggcatca aaaaactcct ctctttttct
    32041 acgaagcgaa aaaaatcaga aaaatttctg taattattgc tttcgctgca agaaagattg
    32101 taacagacag agaagattac accaattcat tttttgtgtt aaagtattct ttattggaat
    32161 aagtaggagg aatatgatgg atatccataa attcgaagtt tttcttgatt tagccgaaac
    32221 aatgaatttc accaaaactg ctgatcgaca attcaccacc caaggaaata tttctaaaca
    32281 gattctttcg ttagaaacag aattagacgt caaattattc gaacgcgccc atagaaaaat
    32341 cgaattgacc gaagctggaa gcttattgct cccatatgta aaaaatgtcg tagaacaata
    32401 ccatgctatg caagaagtgc ttgaaaccta tacgaaagac aacaatcttt cattaaatat
    32461 cttaacgata ccgacaatga tcaattataa aggtttttcc aaaatcaccg agtttttgaa
    32521 acgccatcct gaatttacag tccagttgaa agaagtggaa agtgtcgaat taacttacaa
    32581 tgacgccaat gaacatagtg acacgatcca ctttgctaga agtttccagg ccccaccaag
    32641 ttcgattgag tggttgccta ctgaaacaga cgattttgta gcagttctac ctaaaaatca
    32701 tcctttagct agtaaaaaga aattggatct aagcgaactg aaaaaggaga actttttgct
    32761 ccttggcccc actacgaact tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt
    32821 tgaaccaaaa atctcttata aaggagcaag aatcgacttg atcatcaata tgattgcaaa
    32881 tgacatgggg attgctattg ttatggaaaa aacagtcaag aatctattaa aagaaaatac
    32941 tgtgattctc cctatttctc caacaacaga gagttatctt gcatttactc gtaaagtcgg
    33001 tgagcattct ttggcttcag atacattttg ggagtattta aatcaagaag aaaactaagc
    33061 cctacatttt ctgtccatct atccacccaa aaaaagagag cgacgcatga ctttcgctct
    33121 ctttcttgca tcatgtcctt gtttcaatga cacctctaac atttctcgta gttcaataag
    33181 aaagcagcga tttttatgcc aaaagaaccg taagaacatt tccctaatca caaaaaaacg
    33241 actaaaaacc cctaaaaacc ttcaatagaa tttttagggg ttttattctt tttcctattg
    33301 tttacttaac cgtatcattg ctgtcgttcc tactccttcg atactgctat aatcaatacg
    33361 tccatcatgc agttctagaa tttttttaac cagtgaaagt cctagcccat tcccagaaat
    33421 ttgacgactt gtatctcctt gataaaattt ttcaaacatt cgatctctgg tctcttcatt
    33481 cattcctaca cctgaatcgg tcacttccag tacaatctct gtggctgtct caaatagttt
    33541 gatatgaatc tgtccattga tttgattgta ctttatggcg ttatccatga tattcagcca
    33601 cacttggtac aaaaactctt cattgcctgt gtaatttact gcagcaagtt cgatatccaa
    33661 ttctagctgt tctttctccc atttaggctg aaaaaaaacg attacttctc tgatttgttc
    33721 atctaatcga tactctttct tttccagttg gatacgctgg ttttccagtt tgttcaattt
    33781 taagatgttc tctgttaatt gtgaaagctg cgtgatactt tcgataatcc gatataaaaa
    33841 tatttggcgt tcttcgtcag agagatttgg ggcttgtaac aattgaacat atccttggat
    33901 tgttgctagt ggtgttttga attcatggga tacactggac acaaagtcat tcctcagtgt
    33961 ctcgatacta ttgagttctt ggaccataac gttgaaatct ttatacagtt gctgtacttc
    34021 tgctactttc tgctgttcat ccattcgaat cgaaaaatct cccgttgcta ccagactcat
    34081 atttgtccct agcgcactga ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc
    34141 tgtacccacg accatactga accccgcaaa gacgaataac gggtataacg ttggttttcc
    34201 agccgattca gcaggcgaga tagaatgtcg agacaataag aaaataaaac cgagaaaaca
    34261 cagcagtgtc actaagatcg aaatgaagac aatactagca aaatagatcc ataattgtga
    34321 aaatagcttt tttttcatct ttaaataacc gccttatagc ctagtccccg tactgtgatg
    34381 atttgaaaat cttccgtctt ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc
    34441 gttcgttctt ctgtattcgt atctaatccc caaatatcat ccatcaactg ttggcgagtg
    34501 aagatcttgt tgggataaga cagtaattta tacagaagca gaaattcttt ttgaggcaga
    34561 atcaacgaat gctgatcttg taacactgta taagcatcta gctctaattt tgtttgtccg
    34621 attatcaagc tactgctgta attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg
    34681 atcatctcat tgacatcgat gggttttgtc atataatcat ctgctcctaa tttgaacccg
    34741 cgtttcttat cctcaaaatc ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg
    34801 gtgctacgta atgagtctag caagtcatag ccatccattt tcggcatcat gatatctgta
    34861 atgattaatt ctatacgtgt cgcttctagt tttttcagcg catcctctcc attcaatgcg
    34921 gtgatgactg aaaagcccgc tcgttccaac acagaacggt aaagcaactg taattgcgga
    34981 tcatcttcta ctactaaaat acgattcatt gatctccctc tgttttttga aaaatttcta
    35041 gattttttct tgtagttcat cttcagttaa catttatttc gtatactacg atcattgttc
    35101 tgatatactt attagtatac cgaatatttc aatacgggag aagggtgatt atgcttcaat
    35161 taaaagatat aaaaaaatat tataaagtcg gcgaaaccac caccaaagca ttggatggtg
    35221 tgtctgtcgc tttcagacaa aaagaattcg tagcgatttt aggtcctagt ggttctggta
    35281 aaacgactat gctgaatgtg attggtggac tagataatta tgattccggt gatatggtaa
    35341 tcaacggaaa gtctactaaa gactttaagg acagtgactg ggatgcctac cgaaacaact
    35401 ctatcggttt catttttcaa agttacaact tgattggtca tctaggcatt attgaaaacg
    35461 ttgaacttgg gatgacatta agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag
    35521 atgcattgca ccgagtcggc ttgacaaatc atatgcataa aaagccaaat cagttatctg
    35581 gtggacaaat gcagcgagtg gccatcgctc gagcactagc caatgaccca gatatccttt
    35641 tgtgcgatga accaaccgga gcgttagata cagaaacaag tatccagatc atggaattga
    35701 tccaagagtt atcgaaagaa aaattagtga tcatggtcac acataatcct gaactggccc
    35761 atcaatacgc agatcgaatc attgaatttt ctgatggtaa gatcttaaca gattcccatc
    35821 ctcacatcga acgaccaaaa gatgatcaat ttaacttacg cagaacaaag atgagttttt
    35881 ggacagcgtt aaagctttcc ttcaacaata tccgaacaaa aaaaggacgt actttcttga
    35941 cctcctttgc ttccagtatt gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg
    36001 gattccaaaa acaaatcgat aacacgcaag ctgaaacgat ggcgaaattt ccgattacta
    36061 tttctaaggt aacgactaac cagactcgtg acgatgccgg attgggtgca agcaaagcgg
    36121 attatccaga cagcaaaacg attactgcaa aagtcagcga agaagataaa gcacagcaca
    36181 cgaataagat tgatcaaaca tacgtcgatt acgtaacaga catcgatcct aacttaagta
    36241 ataatatcgg atttacccgt actacaggaa tcaatctttt acgtgatgta aatggcaaag
    36301 tacagcctgt cagcttctcg aatcaaaatc ctgatacgga atctctttct ttatctagta
    36361 cgatgtcagc aatgacaggt gtgggagtgt caagtttccc tacacagctt gacactagca
    36421 aagaaaactt tttaaaagac aattattctt tacttgccgg aagttatcca gcctcagcaa
    36481 cagatgtcgt gctgatcgta gacggcaata ataatactaa tatcaacgca ttgaagaatc
    36541 ttggatttga tgtgaaagaa gacgaaaaac ttgattttga tgatatcgtt ggtacaacat
    36601 tcaaattagt aaacaacaat acttactata caaaacttcc aactgggaat tttatcccta
    36661 atacagatta tgatgcgatg tatcagaatg cttctgatga attgaagatc tcggggatcc
    36721 tacgcgtgaa atcttcttct acaatgaatc tcttatctcc aggaatcgct tatagcgatc
    36781 aattaacgac tcaaatcgtc aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag
    36841 acagtggtgt aaatgtctta actactgaaa aagttgacga aaatgccaaa caaacattgc
    36901 ttagttattt aggtggagac agcttgcctt caagtatcat gatttatccg aacaattttg
    36961 aagacaaaga aaagatcttg gattatctag atgattacaa taaaggcaaa tcagatgaag
    37021 ataaaatcat ttatactgat ttagctggaa cgatgactga actgactggc ggattgatgg
    37081 atgctatcac ttatgtcctt attgcttttg cagggatctc tttagtaaca agtatgatca
    37141 tgatcagtat cattacttat acatctgtga ttgaacggac aaaagaaatc ggtgtactaa
    37201 aagctttagg tgcacgtaaa aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt
    37261 taggtatttc ttctgggata ttaggtgtgt ttattgcatg gctagctacg ttccctatca
    37321 atagcatctt atacaacatg acggatctaa aaaatgttgc tcaactaaat cctgtccatg
    37381 cgattattct agtgatcgtc tctactatat tgacgatgct tggcggtcat cttcctgcac
    37441 gaatggcagc gaaaaaagac gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc
    37501 acatttagat ttgagtcata agagcgctga aatagtagaa aacagcaaga aacgaggaag
    37561 taatatgttc cttgtttcgc gctgtttttt ttaattattt tttataattc tggtatactt
    37621 ttctttcctt tgtaccacaa ctcttctggc gtatacctgt cttttccatt tatgaatact
    37681 gttccatctg cttcaatcgt tacttcagtc ccaatccaca tattacgaga aagtgcgaga
    37741 agattttttc tttttcctct tccttttttt ccaaattgca aggcatcggt tatctcttct
    37801 gtccaatgag atttcacgta ttttacttct ccgttttcaa atgtaacttt caaaatattc
    37861 gctatatttt ccacttttac tgctttagga aatgactcct ccattttttt cactttcctt
    37921 cctccaacac taagtatgcg ctttctttta gtgataagta tatcatagtt tattctatta
    37981 tcaaaaaaac gcatccattc cattgtagaa tactttaccg aacaagttga attgttttcc
    38041 tctttttcac cttttactat agataggtaa agacaactaa ggaggaatct ctcatgacag
    38101 atcaaccgta tgatttaaga aaagtcatcg aagaactaaa aacacttcca ggtcaatatc
    38161 acgaaacaga tattgaaatc gatcctgaag cagaattatc tggtgtttac cgttatatcg
    38221 gtgctggcgg tacagtaaaa cgtccgactc aagaaggacc aactatgact ttcaacaata
    38281 tcaaaggttt ccccggcaca cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag
    38341 gtgaaatttt gcaccatgat tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa
    38401 atccaatcaa acctgtgaaa gtagacaaag cagacgctcc agcacaagaa gtcgttcatc
    38461 tagctaccga tgcagacttc gatattcgca acattttgcc tgcacctaca aatactcctg
    38521 aagatgctgg tccatacatc acaatgggtg tagtcttagg atcagatcca gaaaaaacaa
    38581 tgagcgatgt aacgatccat cgtatggttc tagaagataa agatacgatc ggtatgtaca
    38641 tcatgcctgg cggacgccac attggtcact tccaaaaaca attcgaagcc ttgaataaac
    38701 caatgcctat cactatcaat attggtttag atccagcaat tgccattggc acgactttcg
    38761 aaccacctac cactccactt ggctacaatg aactatggtg tgcaggagct ttgcgtaacg
    38821 aacctattca aatcgttgat ggtatagcag tcgatgaagt gggtattgct cgtgctgaat
    38881 tcatcattga agcagaaatc atgccaaatg aaacaatgca agaagatatc aacacgaact
    38941 caggaaaagc aatgccagaa tttcctggct atactgggga tgctaatcca gctgtacaag
    39001 tagtgaaagt caaagcagtt actcaccgaa aagacaatcc aatcatgcaa acaacgattg
    39061 gtcccagtga agaacacgta tctatggccg gtattcctac agaagcaagt attcttgcct
    39121 tggtcgataa agctattcca ggaaaagtag taaatgtcta caatcctcct gcaggtggtg
    39181 gtaaattgat gaccatcatg caaatccgta aagaaaacga agcggatgaa ggtattcagc
    39241 gtcaagcagc tattcttgct ttatcagcct tcaaagaatt gaagacagtc attttagtcg
    39301 atgaagatgt tgatattttc gacatgaacg atgtagtttg gactttcaac actcgtttcc
    39361 aaggagataa agatatcatc gtgatcccag gaatgaggaa ccatccattg gacccatcag
    39421 aattaccaga atataatcct ggctctattc gtttccgtgg gataagtgca aaaactatct
    39481 ttgacggtac agttcctttc gatatgaaag aacattttgt acgtgcaaaa ttccaagaag
    39541 tagaagattg ggaaaaatat ttgaaataat gaatgatctg caactgtttt gacatcataa
    39601 aaaggagtgc ggaaaatgga acaattggca caacggaaga aaaaaatcgt gattggtgta
    39661 agtggcgctt caggcaccat ttatgccatc aacctagtga agaaactaaa agaatatccg
    39721 atgatagaaa cacacggaat catcagtcca tgggcaaaac agaacttaaa gctggaatcc
    39781 gacttatcac taactgaatt cgaaagcctg tttgattacc attattcaaa caaagattta
    39841 ggtgctgctg tcgccagcgg atcatttttg accgatggaa tgatcatcgt accggcaagt
    39901 atgaaaacag tcgcaggtat ctctgttggt ttaggcgaca acctgattgc acgtgctgct
    39961 gatgtaacaa tgaaggagca acgaaagtta gtcattgttc ctcgtgaaac gccattaaat
    40021 gccattcact tggaaaactt gactaaatta gcacgactag gtgtgcaaat cataccacca
    40081 attcctgctt tttataatca gcctcaaacg atccaagatc ttgttaatca ccatacaatg
    40141 aaacttttgg atcaatttga aattgtaaat gagcattcaa aacgttggca gggtgattaa
    40201 tatggaaaaa gaaacgatag ttgaagtaac caaactaggt ttcacgatcc aagctgctgt
    40261 taaagtaatc ggtaaagact tattgatcac tttaacaggc ggagatacac cacatatcgg
    40321 aacagtcact acattttctc gtgatacaga gatgcaaact gtacgtttcc ctagccacga
    40381 tggtcggttc cataaagacg atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc
    40441 cgttccggga agttgtacga tcacttccgg tatccatgtt gaccatatct ccaatgcaca
    40501 aatcaacgcc tcttttccga tgtcagaaga gttaggttac aagattttag attggttgga
    40561 aaaaatagat ttccatatca aagaaccagt ttattataaa gatggtgaaa aaccattatg
    40621 aaagaagagg ttgtgaaatc agcgttatga cctgagtatg agagaaaaaa atggaaaaat
    40681 agcttctcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacgccgtt
    40741 tatcaagagg ttgtgataaa gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat
    40801 gggtttccac atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg
    40861 tttatcaaga ggttgtgaca cgtcttttgt cacaacctct tttatttttg cttcactgta
    40921 actgctacaa tatcaccgat ttcttttccg atgtttttac gaatatcttt tcgtagtcca
    40981 aggatgtagc agactgaacc atctggattt ttcacgccca tattcacgat actgccttca
    41041 tatggcacat tgtcaaaagt tgcttggact ttaactcttc cagcaccgaa ttcttttctt
    41101 atatcatacg gaaaaataat atatgccccg cccttgccga cttcggaagc atagattttt
    41161 gattcaaaag aataaatttc cctcatttta tttctcctat tttttacatc tagtatagca
    41221 tagcaaaaaa aagaaggaac tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa
    41281 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcaacaaac atgagaagct
    41341 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctcttcctt
    41401 aaacggtgtt caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt
    41461 tgttggaatt cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt
    41521 ggattttcag tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc
    41581 atcgtacttt gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat
    41641 tctacccatg tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta
    41701 tcagcaatat tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc
    41761 gtcacttttt cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca
    41821 gttgcttttg gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca
    41881 ttccctaaag ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt
    41941 ttttctgttt ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg
    42001 atcaaactta gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat
    42061 gtttttttag cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca
    42121 ataatcatgc attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg
    42181 caataataaa gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg
    42241 acagtgtggc attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt
    42301 tgcttgggat atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa
    42361 tcgagcttgc gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat
    42421 ttggcaatgc attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca
    42481 tacggatcgc ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa
    42541 atggtgcagt tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt
    42601 atttatcgaa aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca
    42661 acaacatcaa gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta
    42721 cgaccatttc tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg
    42781 tgaatgtaga tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac
    42841 gccatgagaa caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc
    42901 ttccccagcc gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg
    42961 ttgataaaat agcggataaa atatcaatgc taggatttga caatggaata acacttttca
    43021 ttaggaagaa tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt
    43081 cacgccaatg gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa
    43141 gtccaataac gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag
    43201 atagcatgat attttgcaat aatggcatcg atactcccac accgcaagct tggatcaaac
    43261 gaccgattag taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa
    43321 agatcgtcat ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg
    43381 ttattgggat catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag
    43441 acgctgaaat atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat
    43501 tcaaaacggt acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg
    43561 gttttccgta aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt
    43621 ttctgaaact actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca
    43681 taagttaata cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt
    43741 ggggcttccc ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta
    43801 agtgatatcg taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt
    43861 cgttcgatcg aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa
    43921 gtagaactga tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag
    43981 gcagaaatcc gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa
    44041 gatatcgaat tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag
    44101 aatgctaaga tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt
    44161 cctgtcgatg aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc
    44221 tcctaagtac caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt
    44281 ttgaacataa gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc
    44341 atcactgttc ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc
    44401 caatttgcat attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa
    44461 ttttcatgtt tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt
    44521 cgtttttctt catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc
    44581 acgtctttga cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg
    44641 gtgggtgtat gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact
    44701 ttcttttcca atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc
    44761 atttttgcaa gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca
    44821 tttttgaacg attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac
    44881 aaaatatatc gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa
    44941 atccattcta tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat
    45001 aaggagatgc ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt
    45061 tatctatacg tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt
    45121 tgattaccgt atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga
    45181 tatcgttaaa ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga
    45241 cgtggcattg gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa
    45301 atgggttgaa gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat
    45361 ggaagcagct cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac
    45421 gatcacttat atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata
    45481 cgatggaatg ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt
    45541 aaaataaggt tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa
    45601 gaaacggaaa aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct
    45661 gattgaacac cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac
    45721 gctagttaga aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag
    45781 ccgtttgaac accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag
    45841 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
    45901 gctgattgaa caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag
    45961 tgacttcaaa atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta
    46021 ttctgttcat caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac
    46081 tgcttcatct ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc
    46141 tgattgattt ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt
    46201 cattagttgc gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat
    46261 aaactgatca atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat
    46321 cactgcatct ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg
    46381 aatgaattca tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc
    46441 tttggcagac aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat
    46501 tttaaaataa aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta
    46561 tatcttctct tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc
    46621 tgaaacgatt cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc
    46681 gctttgtact gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat
    46741 gaatgaacta ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag
    46801 cactttttta cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac
    46861 tacagctatt atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc
    46921 ttttccttct tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa
    46981 acgatgcgtg ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc
    47041 tgaaatcgtt agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata
    47101 agccaatttc tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa
    47161 ttcggtaaat aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga
    47221 atttccacca agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct
    47281 accattgagc cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc
    47341 gatcagccat tcctctaatt ggctggatgt tgtactttca tttctgttct tttcattgaa
    47401 aggatcagta ccaggattga atccttggtc agttaatcgt tggtttttct cctccatttt
    47461 ctttcgctgc acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg
    47521 atcaataaaa ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac
    47581 gacgatacat attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact
    47641 ctgcttcaca tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc
    47701 aggaaaaaaa tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta
    47761 ataagtacag cccaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc
    47821 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga
    47881 gtaataagag cgctagtcag aaaatagctt ctcatatttt ctaggctagt gagcttatta
    47941 ccgagaagca gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc
    48001 gagtattaag aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc
    48061 tctctcctta gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat
    48121 tttctaattt cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca
    48181 aaggagttcg acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg
    48241 catttctcat gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc
    48301 attacaatcg gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc
    48361 cttcccttta tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt
    48421 tcgcttattt acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc
    48481 tttcaaattt cattctttat cttatttcag gattttctat gctgatttta ctgagatatg
    48541 tcaatgttcg aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta
    48601 ttcaatactg gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat
    48661 gtatgatccc tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg
    48721 tctcagtggg acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt
    48781 atatcccttt ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc
    48841 aactgattaa gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag
    48901 ctattgttta cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa
    48961 aggcgataga tctggatggc agttattggc tccattaccc taaggccttt cctatattga
    49021 tattatcagg ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa
    49081 tcgtgttgta cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga
    49141 cattgatcga aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg
    49201 tgccatttac gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac
    49261 aaaagtggtt ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc
    49321 agaacttata ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt
    49381 atctattcga tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca
    49441 gcttgttcgt tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag
    49501 acaataaata cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa
    49561 cacatccagg cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata
    49621 tccctgttat cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt
    49681 atgaagtaac agatatcggg accccaatcg tgaagcagca aaaaggaatc aacgagctca
    49741 cgcttcatta tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct
    49801 ggtttacctt gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa
    49861 aagggataaa tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt
    49921 attttaatca gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga
    49981 tttccacccc gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat
    50041 attttttaac ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat
    50101 tttgttccca accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga
    50161 caacatacga atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt
    50221 ctactggatt attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt
    50281 tttccaagtt tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt
    50341 ggcagctcga gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc
    50401 gtgtctgata atcttctttc ttttgaaatt gggagacggt aaggacctga ctttccaatg
    50461 tttttttcgt aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt
    50521 cccaactcca aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa
    50581 aattatcttt acggataggt acttgattct ttaccggaca gaacttcatt agcttcgcag
    50641 gttctacttc ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat
    50701 cttctaacag gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc
    50761 agctatgcgt gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac
    50821 aaaagggttg tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa
    50881 ctcctcagta ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact
    50941 tggtgcaggg cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc
    51001 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg
    51061 acttgatgca ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt
    51121 taccagctgt cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa
    51181 atacgatcat gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag
    51241 ctaatttcgt tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct
    51301 gattcatttg gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt
    51361 ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa
    51421 caacttctct tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata
    51481 ccggatcttt tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt
    51541 gttctatacc taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg
    51601 gctctggaat ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg
    51661 cttttggtcc ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg
    51721 ctcgtttcca ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc
    51781 gagtcataca agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct
    51841 ggaccacttg ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt
    51901 cattgtcgta atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat
    51961 ttgctaagaa atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat
    52021 ctccacctag acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa
    52081 atgaatggac ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg
    52141 aataaatgac atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta
    52201 cacctgttag aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct
    52261 tatcaggatc gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac
    52321 caatcatttg tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt
    52381 gcgacatatc ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg
    52441 gatcttgccc aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc
    52501 caagctgtgt atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta
    52561 aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg
    52621 caatccataa accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa
    52681 tcaatgccac gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga
    52741 aaaatgacaa agcatccggc aattttctat aattatagta cttattataa gccattgccc
    52801 cgacaaatcc agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata
    52861 cacttgtaaa aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat
    52921 cttcattcgc aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta
    52981 aaataaatga gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc
    53041 ctattgctaa agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa
    53101 ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc
    53161 ctatactgat catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc
    53221 caaatttttg ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc
    53281 aatcggtttc gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt
    53341 tatttttctg tctataccat tctataaaac ctataatacg tgtgttccct tctataattt
    53401 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
    53461 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
    53521 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
    53581 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
    53641 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
    53701 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
    53761 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
    53821 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
    53881 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
    53941 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
    54001 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
    54061 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
    54121 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
    54181 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
    54241 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
    54301 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
    54361 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
    54421 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
    54481 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
    54541 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
    54601 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
    54661 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
    54721 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
    54781 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
    54841 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
    54901 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
    54961 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
    55021 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
    55081 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
    55141 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
    55201 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
    55261 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
    55321 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
    55381 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
    55441 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
    55501 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
    55561 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
    55621 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
    55681 tctcagtcac agatgaacaa gttactttcg agttattaga aggttctcct ctagaaattt
    55741 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
    55801 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
    55861 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
    55921 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
    55981 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
    56041 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
    56101 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
    56161 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
    56221 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
    56281 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
    56341 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
    56401 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat
    56461 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
    56521 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
    56581 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
    56641 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
    56701 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
    56761 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
    56821 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
    56881 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
    56941 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
    57001 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
    57061 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
    57121 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
    57181 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
    57241 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
    57301 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
    57361 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
    57421 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
    57481 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
    57541 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
    57601 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
    57661 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
    57721 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
    57781 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
    57841 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac
    57901 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
    57961 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
    58021 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
    58081 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
    58141 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
    58201 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
    58261 gcagttggcg caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
    58321 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
    58381 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
    58441 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
    58501 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
    58561 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
    58621 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    58681 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
    58741 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
    58801 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
    58861 gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
    58921 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
    58981 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
    59041 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
    59101 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
    59161 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
    59221 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
    59281 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
    59341 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
    59401 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
    59461 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
    59521 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
    59581 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
    59641 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
    59701 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
    59761 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
    59821 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
    59881 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
    59941 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
    60001 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
    60061 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
    60121 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
    60181 atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg
    60241 acaaaacgtc ggctgaaaga cttatttttt tatttttctt ctaaactttt ttctacctca
    60301 atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact
    60361 gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg
    60421 acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt
    60481 ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa
    60541 acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag
    60601 aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt
    60661 gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat
    60721 tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc
    60781 caagctgata attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc
    60841 acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat
    60901 tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa
    60961 actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa
    61021 cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag
    61081 tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct
    61141 agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg
    61201 atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata
    61261 tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg
    61321 aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa
    61381 gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca
    61441 aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga
    61501 agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca
    61561 aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc
    61621 gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca
    61681 gatttactca ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag
    61741 tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt tgagtttatt
    61801 gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc
    61861 ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac
    61921 atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca
    61981 atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt
    62041 tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt
    62101 cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga
    62161 tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg
    62221 aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt
    62281 tttctttgaa tacatagtct accatattga aatctggata agttaatgga tgaacttcga
    62341 tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac
    62401 cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt
    62461 gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc
    62521 caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa
    62581 cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca
    62641 ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta
    62701 cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt
    62761 ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc
    62821 cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac
    62881 gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac
    62941 cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt
    63001 cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga
    63061 taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt
    63121 ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt
    63181 tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc
    63241 aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt
    63301 tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt
    63361 ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca
    63421 tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa
    63481 aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc
    63541 cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat
    63601 cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa
    63661 taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat
    63721 tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta
    63781 ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa
    63841 ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat
    63901 ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta
    63961 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
    64021 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc
    64081 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
    64141 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
    64201 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
    64261 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
    64321 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
    64381 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
    64441 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
    64501 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
    64561 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
    64621 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
    64681 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc
    64741 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
    64801 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg
    64861 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
    64921 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
    64981 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
    65041 tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac
    65101 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
    65161 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
    65221 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
    65281 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
    65341 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
    65401 gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
    65461 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
    65521 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
    65581 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
    65641 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
    65701 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
    65761 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
    65821 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
    65881 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
    65941 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
    66001 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
    66061 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
    66121 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
    66181 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
    66241 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
    66301 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
    66361 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
    66421 ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
    66481 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
    66541 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
    66601 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
    66661 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
    66721 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
    66781 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
    66841 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
    66901 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
    66961 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
    67021 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
    67081 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
    67141 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
    67201 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca
    67261 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    67321 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    67381 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    67441 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    67501 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    67561 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    67621 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    67681 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    67741 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    67801 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    67861 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    67921 cgtaat
//
LOCUS       DANKHK010000004        58409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000004 DANKHK010000000
VERSION     DANKHK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            280..651
                     /locus_tag="PW109_000193"
     CDS             280..651
                     /locus_tag="PW109_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2510076.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(925..1617)
                     /locus_tag="PW109_000194"
     CDS             complement(925..1617)
                     /locus_tag="PW109_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2510077.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     GQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1620..2405)
                     /locus_tag="PW109_000195"
     CDS             complement(1620..2405)
                     /locus_tag="PW109_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2510078.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2642..4042
                     /gene="lacG"
                     /locus_tag="PW109_000196"
     CDS             2642..4042
                     /gene="lacG"
                     /locus_tag="PW109_000196"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2510079.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4067..4384
                     /locus_tag="PW109_000197"
     CDS             4067..4384
                     /locus_tag="PW109_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2510080.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4397..6070
                     /locus_tag="PW109_000198"
     CDS             4397..6070
                     /locus_tag="PW109_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2510081.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6084..7715
                     /locus_tag="PW109_000199"
     CDS             6084..7715
                     /locus_tag="PW109_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2510082.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8206..9771)
                     /gene="guaA"
                     /locus_tag="PW109_000200"
     CDS             complement(8206..9771)
                     /gene="guaA"
                     /locus_tag="PW109_000200"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2510083.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9830..10753)
                     /locus_tag="PW109_000201"
     CDS             complement(9830..10753)
                     /locus_tag="PW109_000201"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2510084.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10949..11554
                     /locus_tag="PW109_000202"
     CDS             10949..11554
                     /locus_tag="PW109_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2510085.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11862..12878
                     /locus_tag="PW109_000203"
     CDS             11862..12878
                     /locus_tag="PW109_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2510086.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13019..14320
                     /locus_tag="PW109_000204"
     CDS             13019..14320
                     /locus_tag="PW109_000204"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2510087.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14366..15028
                     /gene="deoC"
                     /locus_tag="PW109_000205"
     CDS             14366..15028
                     /gene="deoC"
                     /locus_tag="PW109_000205"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2510088.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15049..15447
                     /locus_tag="PW109_000206"
     CDS             15049..15447
                     /locus_tag="PW109_000206"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2510089.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15513..16595
                     /locus_tag="PW109_000207"
     CDS             15513..16595
                     /locus_tag="PW109_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2510090.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16807..18369
                     /locus_tag="PW109_000208"
     CDS             16807..18369
                     /locus_tag="PW109_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510091.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18362..19495
                     /locus_tag="PW109_000209"
     CDS             18362..19495
                     /locus_tag="PW109_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510092.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19495..20451
                     /locus_tag="PW109_000210"
     CDS             19495..20451
                     /locus_tag="PW109_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510093.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20550..21716
                     /gene="deoB"
                     /locus_tag="PW109_000211"
     CDS             20550..21716
                     /gene="deoB"
                     /locus_tag="PW109_000211"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2510094.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21740..22558
                     /locus_tag="PW109_000212"
     CDS             21740..22558
                     /locus_tag="PW109_000212"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2510095.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22575..23282
                     /gene="deoD"
                     /locus_tag="PW109_000213"
     CDS             22575..23282
                     /gene="deoD"
                     /locus_tag="PW109_000213"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2510096.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23580..24266)
                     /gene="gpmA"
                     /locus_tag="PW109_000214"
     CDS             complement(23580..24266)
                     /gene="gpmA"
                     /locus_tag="PW109_000214"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2510097.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24438..25019)
                     /locus_tag="PW109_000215"
     CDS             complement(24438..25019)
                     /locus_tag="PW109_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2510098.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25152..25835)
                     /gene="rpiA"
                     /locus_tag="PW109_000216"
     CDS             complement(25152..25835)
                     /gene="rpiA"
                     /locus_tag="PW109_000216"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2510099.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26154..26567
                     /gene="rpsL"
                     /locus_tag="PW109_000217"
     CDS             26154..26567
                     /gene="rpsL"
                     /locus_tag="PW109_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2510100.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26651..27121
                     /gene="rpsG"
                     /locus_tag="PW109_000218"
     CDS             26651..27121
                     /gene="rpsG"
                     /locus_tag="PW109_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2510101.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27215..29302
                     /gene="fusA"
                     /locus_tag="PW109_000219"
     CDS             27215..29302
                     /gene="fusA"
                     /locus_tag="PW109_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2510102.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29474..30661
                     /gene="tuf"
                     /locus_tag="PW109_000220"
     CDS             29474..30661
                     /gene="tuf"
                     /locus_tag="PW109_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2510103.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31068..31376
                     /gene="rpsJ"
                     /locus_tag="PW109_000221"
     CDS             31068..31376
                     /gene="rpsJ"
                     /locus_tag="PW109_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2510104.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31407..32036
                     /gene="rplC"
                     /locus_tag="PW109_000222"
     CDS             31407..32036
                     /gene="rplC"
                     /locus_tag="PW109_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2510105.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32064..32687
                     /gene="rplD"
                     /locus_tag="PW109_000223"
     CDS             32064..32687
                     /gene="rplD"
                     /locus_tag="PW109_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2510106.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32687..32977
                     /gene="rplW"
                     /locus_tag="PW109_000224"
     CDS             32687..32977
                     /gene="rplW"
                     /locus_tag="PW109_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2510107.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33016..33849
                     /gene="rplB"
                     /locus_tag="PW109_000225"
     CDS             33016..33849
                     /gene="rplB"
                     /locus_tag="PW109_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2510108.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33892..34170
                     /gene="rpsS"
                     /locus_tag="PW109_000226"
     CDS             33892..34170
                     /gene="rpsS"
                     /locus_tag="PW109_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2510109.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34193..34540
                     /gene="rplV"
                     /locus_tag="PW109_000227"
     CDS             34193..34540
                     /gene="rplV"
                     /locus_tag="PW109_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2510110.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34554..35210
                     /gene="rpsC"
                     /locus_tag="PW109_000228"
     CDS             34554..35210
                     /gene="rpsC"
                     /locus_tag="PW109_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2510111.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35213..35647
                     /gene="rplP"
                     /locus_tag="PW109_000229"
     CDS             35213..35647
                     /gene="rplP"
                     /locus_tag="PW109_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2510112.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35637..35825
                     /gene="rpmC"
                     /locus_tag="PW109_000230"
     CDS             35637..35825
                     /gene="rpmC"
                     /locus_tag="PW109_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2510113.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35850..36116
                     /gene="rpsQ"
                     /locus_tag="PW109_000231"
     CDS             35850..36116
                     /gene="rpsQ"
                     /locus_tag="PW109_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2510114.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36174..36542
                     /gene="rplN"
                     /locus_tag="PW109_000232"
     CDS             36174..36542
                     /gene="rplN"
                     /locus_tag="PW109_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2510115.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36579..36887
                     /gene="rplX"
                     /locus_tag="PW109_000233"
     CDS             36579..36887
                     /gene="rplX"
                     /locus_tag="PW109_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2510116.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36915..37454
                     /gene="rplE"
                     /locus_tag="PW109_000234"
     CDS             36915..37454
                     /gene="rplE"
                     /locus_tag="PW109_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2510117.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37473..37658
                     /locus_tag="PW109_000235"
     CDS             37473..37658
                     /locus_tag="PW109_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2510118.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37694..38092
                     /gene="rpsH"
                     /locus_tag="PW109_000236"
     CDS             37694..38092
                     /gene="rpsH"
                     /locus_tag="PW109_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2510119.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38124..38660
                     /gene="rplF"
                     /locus_tag="PW109_000237"
     CDS             38124..38660
                     /gene="rplF"
                     /locus_tag="PW109_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2510120.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38821..39177
                     /gene="rplR"
                     /locus_tag="PW109_000238"
     CDS             38821..39177
                     /gene="rplR"
                     /locus_tag="PW109_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2510121.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39198..39698
                     /gene="rpsE"
                     /locus_tag="PW109_000239"
     CDS             39198..39698
                     /gene="rpsE"
                     /locus_tag="PW109_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2510122.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39713..39892
                     /gene="rpmD"
                     /locus_tag="PW109_000240"
     CDS             39713..39892
                     /gene="rpmD"
                     /locus_tag="PW109_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2510123.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39936..40376
                     /gene="rplO"
                     /locus_tag="PW109_000241"
     CDS             39936..40376
                     /gene="rplO"
                     /locus_tag="PW109_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2510124.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40376..41671
                     /gene="secY"
                     /locus_tag="PW109_000242"
     CDS             40376..41671
                     /gene="secY"
                     /locus_tag="PW109_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2510125.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41733..42380
                     /locus_tag="PW109_000243"
     CDS             41733..42380
                     /locus_tag="PW109_000243"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2510126.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42571..42789
                     /gene="infA"
                     /locus_tag="PW109_000244"
     CDS             42571..42789
                     /gene="infA"
                     /locus_tag="PW109_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2510127.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42822..42938
                     /gene="rpmJ"
                     /locus_tag="PW109_000245"
     CDS             42822..42938
                     /gene="rpmJ"
                     /locus_tag="PW109_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2510128.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42956..43321
                     /gene="rpsM"
                     /locus_tag="PW109_000246"
     CDS             42956..43321
                     /gene="rpsM"
                     /locus_tag="PW109_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2510129.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43349..43738
                     /gene="rpsK"
                     /locus_tag="PW109_000247"
     CDS             43349..43738
                     /gene="rpsK"
                     /locus_tag="PW109_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2510130.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43818..44756
                     /locus_tag="PW109_000248"
     CDS             43818..44756
                     /locus_tag="PW109_000248"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2510131.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44784..45164
                     /gene="rplQ"
                     /locus_tag="PW109_000249"
     CDS             44784..45164
                     /gene="rplQ"
                     /locus_tag="PW109_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2510132.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45436..46275
                     /locus_tag="PW109_000250"
     CDS             45436..46275
                     /locus_tag="PW109_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2510133.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46251..47120
                     /locus_tag="PW109_000251"
     CDS             46251..47120
                     /locus_tag="PW109_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2510134.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47110..47907
                     /locus_tag="PW109_000252"
     CDS             47110..47907
                     /locus_tag="PW109_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2510135.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48110..48856
                     /gene="truA"
                     /locus_tag="PW109_000253"
     CDS             48110..48856
                     /gene="truA"
                     /locus_tag="PW109_000253"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2510136.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49311..51980)
                     /locus_tag="PW109_000254"
     CDS             complement(49311..51980)
                     /locus_tag="PW109_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2510137.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52218..52406)
                     /locus_tag="PW109_000255"
     CDS             complement(52218..52406)
                     /locus_tag="PW109_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2510138.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52676..53038
                     /locus_tag="PW109_000256"
     CDS             52676..53038
                     /locus_tag="PW109_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2510139.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53090..54772
                     /locus_tag="PW109_000257"
     CDS             53090..54772
                     /locus_tag="PW109_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2510140.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54889..56925
                     /gene="recG"
                     /locus_tag="PW109_000258"
     CDS             54889..56925
                     /gene="recG"
                     /locus_tag="PW109_000258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2510141.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56974..57975
                     /gene="plsX"
                     /locus_tag="PW109_000259"
     CDS             56974..57975
                     /gene="plsX"
                     /locus_tag="PW109_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2510142.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58097..58339
                     /gene="acpP"
                     /locus_tag="PW109_000260"
     CDS             58097..58339
                     /gene="acpP"
                     /locus_tag="PW109_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2510143.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        18634 a        10645 c        12754 g        16376 t
ORIGIN      
        1 ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt
       61 ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga
      121 aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat agatttaata
      181 agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa
      241 ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat
      301 aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa
      361 gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa
      421 atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt
      481 gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc
      541 cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat
      601 aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta accattacgc
      661 ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag
      721 aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg
      781 gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt
      841 atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt
      901 ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt
      961 ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac
     1021 tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc
     1081 agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag
     1141 tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc
     1201 atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc
     1261 aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggc cagtcagctg
     1321 gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag
     1381 catacgataa tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg
     1441 ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat
     1501 atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc
     1561 atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt
     1621 tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca
     1681 tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc
     1741 aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat
     1801 ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta
     1861 gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt
     1921 cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg
     1981 ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta
     2041 tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta
     2101 cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt
     2161 tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa
     2221 gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat
     2281 tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta
     2341 acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc
     2401 aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc
     2461 taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt
     2521 gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc
     2581 gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta
     2641 catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga
     2701 aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca
     2761 agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga
     2821 attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt
     2881 tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga
     2941 agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa
     3001 acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta
     3061 tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat
     3121 ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga
     3181 ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa
     3241 ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa
     3301 atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc
     3361 gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc
     3421 tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt
     3481 tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca
     3541 ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg
     3601 gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg
     3661 aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa
     3721 aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga
     3781 tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt
     3841 ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc
     3901 gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt
     3961 tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag
     4021 tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga
     4081 attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt
     4141 gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga
     4201 agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc
     4261 atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac
     4321 cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg
     4381 ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt
     4441 tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat
     4501 gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg
     4561 ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg
     4621 tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg
     4681 tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag
     4741 tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac
     4801 aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg
     4861 tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac
     4921 cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg
     4981 cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct
     5041 atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt
     5101 ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta
     5161 tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact
     5221 gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt
     5281 tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc
     5341 ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt
     5401 gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa
     5461 gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc
     5521 agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc
     5581 ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt
     5641 cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc
     5701 cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga
     5761 aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt
     5821 aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc
     5881 tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta
     5941 ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg
     6001 agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca
     6061 atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat
     6121 ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg
     6181 aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat
     6241 attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact
     6301 atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag
     6361 ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta
     6421 aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc
     6481 ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa
     6541 agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca
     6601 atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag
     6661 aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac
     6721 ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt
     6781 ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt
     6841 atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac
     6901 ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg
     6961 atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac
     7021 cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata
     7081 atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg
     7141 caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca
     7201 accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag
     7261 caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca
     7321 gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg
     7381 aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg
     7441 catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg
     7501 taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg
     7561 aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc
     7621 caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga
     7681 cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat
     7741 cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc
     7801 ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc
     7861 agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat
     7921 gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta
     7981 gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac
     8041 agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata
     8101 gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa
     8161 gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt cccactccac
     8221 tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt
     8281 tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt
     8341 cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc
     8401 acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg
     8461 gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc
     8521 tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg
     8581 ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc
     8641 tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc
     8701 tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt
     8761 atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc
     8821 gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga
     8881 tacacctttc aacttgtcta agaagcgatc tcttgcatcg actttgatga tgttcaagcc
     8941 gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg
     9001 gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac
     9061 actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg
     9121 gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca
     9181 aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc
     9241 atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc
     9301 tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct
     9361 catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac
     9421 ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt
     9481 gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat
     9541 ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat
     9601 gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc
     9661 ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta
     9721 gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac
     9781 ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc
     9841 gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc
     9901 ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt
     9961 ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg
    10021 gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca
    10081 gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct
    10141 gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga
    10201 tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta
    10261 tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc atatcataac
    10321 tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa
    10381 gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg
    10441 tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa
    10501 taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt
    10561 caaattcttt catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat
    10621 cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag
    10681 gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca
    10741 ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa
    10801 taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat ctttataata
    10861 gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt
    10921 catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga
    10981 tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt
    11041 aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga
    11101 tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta
    11161 tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat
    11221 taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt
    11281 tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt
    11341 aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata
    11401 tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc
    11461 gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa
    11521 aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga
    11581 aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt
    11641 tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt
    11701 tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa
    11761 aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt
    11821 ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag
    11881 atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag
    11941 gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt
    12001 ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga
    12061 tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt
    12121 tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga
    12181 tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa
    12241 atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt
    12301 taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg
    12361 gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga
    12421 aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga
    12481 aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag
    12541 gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg
    12601 ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc
    12661 cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc
    12721 aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga
    12781 tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa
    12841 ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt
    12901 aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt
    12961 ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat
    13021 gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat
    13081 cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt
    13141 attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc
    13201 gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa
    13261 gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag
    13321 tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact
    13381 agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg
    13441 cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga
    13501 caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc
    13561 cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac
    13621 cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat
    13681 gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga
    13741 acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca
    13801 aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt
    13861 tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga
    13921 atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc
    13981 gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa
    14041 acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct
    14101 aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca
    14161 taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga
    14221 cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc
    14281 aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac
    14341 cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt
    14401 gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca
    14461 tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg
    14521 tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac
    14581 aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat
    14641 caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt
    14701 tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga
    14761 tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa
    14821 aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga
    14881 aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc
    14941 aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt
    15001 atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc
    15061 aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc
    15121 cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac
    15181 attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca
    15241 gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct
    15301 atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg
    15361 gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct
    15421 tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa
    15481 ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag
    15541 gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg
    15601 attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag
    15661 gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga
    15721 aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat
    15781 cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta
    15841 ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact
    15901 ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag
    15961 acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag
    16021 tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag
    16081 ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg
    16141 ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa
    16201 acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa
    16261 aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat
    16321 cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac
    16381 tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc
    16441 ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac
    16501 aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg
    16561 acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac
    16621 acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc
    16681 ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac
    16741 gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa
    16801 aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga
    16861 acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg
    16921 cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag
    16981 ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa
    17041 gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact
    17101 gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa
    17161 aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat
    17221 gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt
    17281 taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa
    17341 attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg
    17401 attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt
    17461 ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg
    17521 atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc
    17581 gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac
    17641 ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg
    17701 cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag
    17761 ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga
    17821 cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat
    17881 atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca
    17941 gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa
    18001 cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa
    18061 gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt
    18121 gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta
    18181 ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat
    18241 gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta
    18301 ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca
    18361 catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt
    18421 aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta
    18481 ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca
    18541 agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt
    18601 caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact
    18661 tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt
    18721 agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga
    18781 agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca
    18841 tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa
    18901 tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg
    18961 attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt
    19021 aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag
    19081 tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg
    19141 tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat
    19201 tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt
    19261 atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg
    19321 tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa
    19381 cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt
    19441 tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg
    19501 actatcgaat taattgcctt gatccttact tctacactgg tttattcaac accgctaatt
    19561 ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag
    19621 ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca
    19681 cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt
    19741 tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca
    19801 gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa
    19861 ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat
    19921 attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt
    19981 ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt
    20041 tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt
    20101 tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag
    20161 tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct
    20221 gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt
    20281 tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca
    20341 gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg
    20401 aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc
    20461 gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca
    20521 ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg
    20581 attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata
    20641 cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt
    20701 taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga
    20761 cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg
    20821 gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca
    20881 aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag
    20941 ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt
    21001 ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat
    21061 atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa
    21121 tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg
    21181 attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg
    21241 acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg
    21301 tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt
    21361 tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg
    21421 atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg
    21481 aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt
    21541 ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga
    21601 aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga
    21661 actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt
    21721 tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt
    21781 tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg
    21841 tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt
    21901 tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa
    21961 gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt
    22021 gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc
    22081 atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat
    22141 caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt
    22201 tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga
    22261 aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga
    22321 aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac
    22381 tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat
    22441 taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac
    22501 tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata
    22561 ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga
    22621 ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc
    22681 cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac
    22741 gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat
    22801 tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa
    22861 aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga
    22921 tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga
    22981 aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg
    23041 aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt
    23101 taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga
    23161 cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag
    23221 aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat
    23281 aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc
    23341 tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt
    23401 taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    23461 agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg
    23521 tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt
    23581 tataggtagt attttttagc aactgttaag tcatcattta attcataaac aagtggttga
    23641 cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct
    23701 agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct
    23761 ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct
    23821 ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct
    23881 tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact
    23941 tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag
    24001 tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct
    24061 aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat
    24121 tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt
    24181 gctagattta catcagccca accagtaaat tggttcaatg cattccattc actaagaccg
    24241 tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc
    24301 tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt
    24361 agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa
    24421 ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata
    24481 tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct
    24541 tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt
    24601 ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc
    24661 aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg
    24721 cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc
    24781 gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt
    24841 gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt
    24901 tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca
    24961 acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg
    25021 gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag
    25081 tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa
    25141 gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg
    25201 acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca
    25261 tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg
    25321 taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg
    25381 aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact
    25441 gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt
    25501 tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc
    25561 ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact
    25621 tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa
    25681 gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc
    25741 atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc
    25801 gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt
    25861 gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt
    25921 ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc
    25981 gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt
    26041 tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga
    26101 accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta
    26161 caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg
    26221 ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa
    26281 aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac
    26341 gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga
    26401 ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact
    26461 taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc
    26521 gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa
    26581 acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga
    26641 ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat
    26701 ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg
    26761 gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga
    26821 tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc
    26881 acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt
    26941 tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga
    27001 agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa
    27061 acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta
    27121 agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag
    27181 caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa
    27241 cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg
    27301 tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca
    27361 aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac
    27421 agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac
    27481 agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca
    27541 atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc
    27601 tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc
    27661 aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga
    27721 agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga
    27781 tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga
    27841 atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata
    27901 cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt
    27961 taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt
    28021 actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg
    28081 gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc
    28141 agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt
    28201 gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga
    28261 acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta
    28321 cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg
    28381 tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt
    28441 cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact
    28501 atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat
    28561 cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa
    28621 agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac
    28681 tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg
    28741 gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg
    28801 tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga
    28861 aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc
    28921 ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg
    28981 tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt
    29041 tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt
    29101 tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc
    29161 tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac
    29221 aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa
    29281 aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca
    29341 aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg
    29401 gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg
    29461 aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg
    29521 tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact
    29581 atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga
    29641 acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca
    29701 ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc
    29761 tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac
    29821 tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa
    29881 caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga
    29941 cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt
    30001 gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt
    30061 tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga
    30121 agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca
    30181 agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac
    30241 agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat
    30301 tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa
    30361 accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga
    30421 agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac
    30481 aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga
    30541 caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt
    30601 ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta
    30661 attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg
    30721 tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag
    30781 tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg
    30841 aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg
    30901 acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta
    30961 agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt
    31021 tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa
    31081 aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa
    31141 tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg
    31201 aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat
    31261 tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg
    31321 atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact
    31381 aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa
    31441 tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc
    31501 caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg
    31561 gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag
    31621 caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg
    31681 aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag
    31741 gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac
    31801 caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta
    31861 accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc
    31921 aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg
    31981 ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt
    32041 aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc
    32101 aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg
    32161 tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa
    32221 accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg
    32281 cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa
    32341 caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg
    32401 tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg
    32461 caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat
    32521 tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg
    32581 tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt
    32641 tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga
    32701 cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata
    32761 cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc
    32821 ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat
    32881 gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga
    32941 ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa
    33001 ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac
    33061 atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa
    33121 ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt
    33181 ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt
    33241 gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac
    33301 tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa
    33361 cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt
    33421 ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc
    33481 cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc
    33541 ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta
    33601 ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt
    33661 aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt
    33721 gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt
    33781 ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa
    33841 aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt
    33901 agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca
    33961 ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt
    34021 tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa
    34081 gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat
    34141 gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga
    34201 acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg
    34261 tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt
    34321 cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc
    34381 agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga
    34441 aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa
    34501 acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc
    34561 aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt
    34621 atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt
    34681 caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg
    34741 taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag
    34801 tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg
    34861 tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat
    34921 tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg
    34981 ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc
    35041 gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt
    35101 acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc
    35161 gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt
    35221 acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg
    35281 cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac
    35341 aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa
    35401 agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat
    35461 gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat
    35521 gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca
    35581 caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga
    35641 aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa
    35701 aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac
    35761 gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta
    35821 agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt
    35881 atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa
    35941 agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg
    36001 atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat
    36061 cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac
    36121 acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc
    36181 aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca
    36241 aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg
    36301 ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg
    36361 ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg
    36421 ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg
    36481 cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat
    36541 aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata
    36601 aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc
    36661 cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt
    36721 ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg
    36781 tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca
    36841 aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag
    36901 gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat
    36961 tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca
    37021 tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag
    37081 aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg
    37141 gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa
    37201 tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg
    37261 gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat
    37321 tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta
    37381 ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc
    37441 cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa
    37501 acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc
    37561 cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata
    37621 aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg
    37681 aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa
    37741 cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat
    37801 cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa
    37861 acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa
    37921 cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa
    37981 agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa
    38041 agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtaa
    38101 aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg
    38161 aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat
    38221 tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca
    38281 ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca
    38341 acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg
    38401 ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag
    38461 tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg
    38521 ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc
    38581 ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag
    38641 aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa
    38701 ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat
    38761 ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt
    38821 gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac
    38881 aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc
    38941 tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat
    39001 aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct
    39061 gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac
    39121 catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag
    39181 aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg
    39241 ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg
    39301 ctttagttgt tgtcggtgac aaaaacggac acgtaggatt tggtacaggt aaagctcaag
    39361 aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac
    39421 ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc
    39481 taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat
    39541 tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca
    39601 acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag
    39661 aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga
    39721 attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt
    39781 taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat
    39841 taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa
    39901 aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg
    39961 ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa
    40021 aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag
    40081 gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg
    40141 tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag
    40201 ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa
    40261 tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct
    40321 ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt
    40381 tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt
    40441 atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc
    40501 ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag
    40561 tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat
    40621 cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg
    40681 ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt
    40741 tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa
    40801 taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt
    40861 tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat
    40921 catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta
    40981 ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact
    41041 agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat
    41101 cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa
    41161 agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa
    41221 tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat
    41281 ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc
    41341 cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca
    41401 aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc
    41461 aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct
    41521 tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag
    41581 cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct
    41641 gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac
    41701 acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag
    41761 gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct
    41821 caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa
    41881 agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag
    41941 agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc
    42001 tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg
    42061 tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct
    42121 gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat
    42181 gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta
    42241 aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac
    42301 aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac
    42361 aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct
    42421 tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga
    42481 tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa
    42541 atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa
    42601 ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac
    42661 caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt
    42721 gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc
    42781 tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag
    42841 taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt
    42901 gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc
    42961 tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat
    43021 ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga
    43081 tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa
    43141 attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga
    43201 aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac
    43261 gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata
    43321 attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg
    43381 tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca ataacactat
    43441 catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg
    43501 tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac
    43561 aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc
    43621 tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg
    43681 tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg
    43741 agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga
    43801 aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag
    43861 aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag
    43921 ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc
    43981 aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc
    44041 aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc
    44101 ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg
    44161 atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc
    44221 atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag
    44281 aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga
    44341 actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg
    44401 agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca
    44461 tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca
    44521 tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag
    44581 acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac
    44641 ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag
    44701 aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc
    44761 ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac
    44821 gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta
    44881 ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac
    44941 gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg
    45001 tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata
    45061 ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa
    45121 gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca
    45181 tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta
    45241 gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt
    45301 tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt
    45361 aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat
    45421 gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta taaataccag
    45481 ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg
    45541 atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga
    45601 ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat
    45661 atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa tcagtttgtt
    45721 ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa
    45781 gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca
    45841 cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt
    45901 gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt
    45961 cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata
    46021 tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa
    46081 ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc
    46141 gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg
    46201 gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc
    46261 gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat
    46321 ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa
    46381 gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag
    46441 tccatattgg agaacgagat attcagccgg atacggataa taagaattta aagcccatcc
    46501 gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag
    46561 caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc
    46621 tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat
    46681 ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc
    46741 cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggc agaaaagaaa
    46801 tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc
    46861 atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa
    46921 tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac
    46981 aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact
    47041 ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag
    47101 gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc
    47161 catcgattgg atccccgagc taaattatta gccagttttt attttatcgg aatcattttt
    47221 ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc
    47281 ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc
    47341 ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg
    47401 ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc
    47461 gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg
    47521 gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt
    47581 tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag
    47641 atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa
    47701 atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa
    47761 gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat
    47821 cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt attattaact
    47881 gtgctactga tctttttaag aggataaacg ttcagtgggc cgctatctat tcttttcttg
    47941 ctgatctgtt tgcaagaaag gatgcaagca gtgtatgaca agaaatatta gggtctgtgg
    48001 cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata
    48061 gaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta
    48121 caaagcaatc attgcgtacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg
    48181 gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat
    48241 cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgatcgggc aagtaatcca
    48301 ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc
    48361 accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata
    48421 tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt
    48481 tcgacgccat tatgcaagtt attttcctta cccacttgat ttggaaaaaa ttcagcgggc
    48541 gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat
    48601 agaggataaa atacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga
    48661 attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg
    48721 aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc
    48781 gaaaaaagat cgtaatgcag caggacctac agcacaccct gaaggacttt atttgtatga
    48841 agtgaaatat gaataagagg ttatgataga agtggtcagc ttcaagaaat aagaaggtat
    48901 ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa gaagctactt
    48961 ctggaacacc gtttaaggaa gaggtcgtga aatcagcgtt ttgacctgag tattagagaa
    49021 acaaacggga aaatagctcc tcatattttg ccgtttgttg agctaatacc gaaggtcagc
    49081 tgattgaaca ccgtttaaga aagaggtcgt gagcctgtca tttaactctg agtcacaaag
    49141 agcaatttca aaaaacagct tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt
    49201 tgataagtga atcatctttt tgttacctat tttttctgta accaaaaaac gatggaacgt
    49261 tgttttgatt gcccttggtg cggacagtct gaacaggttc catcgtttct ttattttcta
    49321 cgtcttcttc tgtgtgtgtt tcgtggttgt tcttctattg ctggatcaat cgtgccataa
    49381 aaggggcgat tgcggtcaag gtaccagaca taaagaataa acaatgcaat acataggaca
    49441 aaaaggattg cacctatcgt aaatccttca ggcaagtaac tgaaagtgac agtatgttta
    49501 cctgctggaa cttttatcat caagaatgcg tctttgaaat tgctgacctt gactggtttc
    49561 ccatcaattt tagctttcca tcctttgtca taaggaatag ttgtcaccaa catggaatct
    49621 tcagcggtat ttacagtccc agaagctttt ctgccagtgg cttccatttc gacacctttt
    49681 tctttgatct gattcattgc ggcttgatag gctacaacat ctagaccaac gactttaggc
    49741 tccatgaaac ttatttcttt cgtcccataa aaacttgcag taaatgtcac tgttgtctct
    49801 tgaggataat agccaagatc atagtattga ccagaaatgt tgatttgcgt ttttcgcgat
    49861 gtaccattga cagtgatcgt cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac
    49921 aaacttaagt aagcttgtgt atttgcaggc acatggactt cccaagtgag ctcttttggc
    49981 tggttatctg ctgtttctga gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc
    50041 aactgattct tcaaagtagg tgtatagaat gtaaaatact gttcatttag accagacaat
    50101 gtattgatca agtttgtctg actggtcaaa ttatctgtca ctggctgctc aacatcatta
    50161 acggacaacg gtgcagtaaa tgctagtggc aatgcattga cattctgata cagtgtatag
    50221 tcgccgcttt gaccagcttg gcggaagcca tatttactga acgtgctttc gtttttagaa
    50281 atgttatatt tgattcctgt aaacccatcc atcagcaaag tattattgtt ataacggata
    50341 ttcaaatttg tccctcttga acggaaacct aatttatcca ggtaagagga agaattacga
    50401 tttcttatag aagaaaacaa actgatacca ctgtaaccat agttgatcga atcgtttgca
    50461 gatactggat ccagattttc caagcggtaa aacgagtcgg aatctttttt tgtttcattt
    50521 acaagagctt tcaagtcagg ataaggatca gtatataaac tgcgggaagc ataattccaa
    50581 tcatcaagaa tgccatggat catactgttc gtattgacca gtgcttcagc agacatgatc
    50641 aataataaca atatactcag atgattgaat gaaagttttt tatgctgata aaatcccaca
    50701 gcaaaagcat aaatccctaa gaaaaccact gtcaatacaa aggaagtcat cgtcacatag
    50761 gtgtagcttc cttcaggttt gatgccccat gccaccgcaa agagagcagc tacgaccaaa
    50821 atgattccta acaacagccc acgatcgtct tgttctatct tttcccatcc ttttcctgca
    50881 agcaggagca ctaagaaaga gaacagatag gcataacgga ataaaaacat attcggtgca
    50941 tgcataccat gccagaacaa attcaatgga acaagataaa aacttgcgat aagtatggcg
    51001 aaaagactgc cgtacaaaaa tttatttttc aacggtactt tccgactggc aaaataaaac
    51061 agacagagga ctaatggtag cagacctgcg taaacgaacg gaatcgatcc gtattttgtc
    51121 gtgtcataaa caccaatcat atttttcatg attagatcca gataagaagt cgcttctgtt
    51181 ttcaacgtag tgatctgact cagtgcctcg ccatttgacc gaagatccag cactgctggc
    51241 aatacaatga tcattgaagc gccgcctgcc agtaaagaag tgataccata aggaacaatc
    51301 gtttttttat attctgacca gtttgtcaat agacgggcga taaaatataa gaaagaaaat
    51361 acgccaatca taaaacccat ataaaaactg gataaaaaca agagtaaata actgataaat
    51421 aaaagtttcg gccgtttaag atccataaca cgatgaatcc ccaaaatcac taatggaaga
    51481 taaacaaatg catccagcca catgatgatc tccgaatgtg cagtgataaa tgagatcaat
    51541 gcgtaactga cgctaagtgc aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga
    51601 aaccaaaaag ccaatccggc acagccgacc ttaagcaaag tcagaacata cagtgcatct
    51661 ggcatcatct ggtttggaaa aaagaacacc agtggcgtga agatcccgcc tagatagtaa
    51721 gaaattaaag ataaataatt caatcctaga gaagcattcc atgtataaaa aatactttgc
    51781 tttccaagaa gaacatttcg gaagctggca tgaaaattag aaaactgtga aaaggcatca
    51841 cttgccaaaa tacttctgct gcttccagga tagattccga tacttagata aacaagtgct
    51901 aaaatcaaaa acggaatcag gaaactagct gctgcgtagc gtccgttttc tctaaagaat
    51961 gactttaaat tttttttcat atttattttc caccaaattt ctatgaagtt gtattcagtt
    52021 tatcatatcc ttgtttttct ctgcttaaaa agagtctagt tttcacaaaa aaataaaatt
    52081 tagaataaat aacttcataa tttgttcgta taacaaaaat aaacgcaaaa aaagggtagg
    52141 aatcaaaagt cccaccctta aaaattttta ttaaataaaa aaccgttcac tatctgaacg
    52201 gtccaagtat cattcaatta aacgcgttca acttttcctg atttcaaagc acgagttgac
    52261 acccaaactt ttttaggttt tccgtcgacc ataacgcgaa ctttttgtaa gttaggttta
    52321 acagtacgtt ttgttgagtt catagcgtgt gaacggttgt ttccactttt tgtcttgcga
    52381 cctgtaaagt aacaaacttt tgccatggta atttcctcct ttgccttgat ttggagcaat
    52441 agaataattc agctcatacc agactaacat atcatataga atgggaattt gcaagtacat
    52501 tttcatagat acaattgaca attgagaaga ataattgtaa attaaacaag gttatacctg
    52561 aaattttgca aggtttttga gtactttggg taactgcctt ttatttcaag ttgcgctatg
    52621 ataaaatatg atagaagaaa aatgggagtt ttttacccgt taaggaggct cattcatggc
    52681 tgtaaaaatt caaacgtcag ctggaaccat tgaaattacc aatgatgtaa ttgcaacagt
    52741 tgttggtggc gccgcgacag atgtgttcgg tgttgtagga atggccagca aaaatcaaat
    52801 taaagataat attactgaga ttttgcgtaa agaaaattac tcaagaggag tagttgtccg
    52861 ccaagaagat aatggtattg ctgtagatgt ttatacgatt atcagctatg gtacgaaaat
    52921 ctctgaagtt tcccgcaacg tccaagaaaa agtgaaatac aatctagagg cattattagg
    52981 cgtaactgcc aattcagtta atgtctttgt acaaggtgtt cgtgtactgc cagactaggc
    53041 aggagactgg ctggtaactc agcagtcgat ctgaaggagg attatctagg tgagtgtaac
    53101 agaaataagt gcaagccagt tccaggaaat ggtccaggca ggtgcgaatc gtctgcaagt
    53161 aaatgcagaa tacgtcaatt cattaaatgt attccctgtg ccagatggcg atacgggaac
    53221 aaatatgaat ctatctatga ctagtggagc taaggcagta gtagattcaa cctcagaaaa
    53281 agtaggagaa ttagcagcaa tgctttcaaa aggattgctg atgggtgcgc gcggaaattc
    53341 cggcgttatt ttgtctcaat tgtttcgtgg attttctaag caaattccag atgttgtctc
    53401 attaaatgct tctgatcttg ctgcagcctt cactcatgga gttgaaactg cgtataaagc
    53461 agtaatgaaa ccagtagaag gaacgatttt gacagtagct agagaagcag cgaaagctgg
    53521 agaaaagaaa gcaaaaagta ctgatgatgt cgttgaagta atgacagctg ttgtaaaagg
    53581 agcaaagcgt gctttagcta aaactccaga cttgcttcca gtcctaaaag aagtcggtgt
    53641 cgtagacagt ggagggcaag gactgctgtt tgtttatgag ggctttttaa gtgcattgaa
    53701 tggagaattc caagcggatg aaacatatga accttctcca gcagaaatgg atgatatggt
    53761 caatgcagaa catcatcgca gtgtccaagg tcaaatggcg acagaagata tccattttgg
    53821 ttactgtaca gaaatcatgg taaaaatcgg agaaggacct acagtcgata gtacattcga
    53881 ttacgaagaa ttccgtaact atttagatgg tataggagat tctttacttg tggtcaatga
    53941 tgacgagatc atcaaagtcc acgtgcatac agaaaaccca ggagaagtct tgaattatgg
    54001 acaaaaattc ggttcattag tcaaaatcaa agtcgacaac atgcgcttgc agcatgagac
    54061 gattttggaa cacgatgaag aagtgaacga gtttgcgaaa aaagaaatac cacgtatccc
    54121 gtatggtatc gtagcgatcg ctgctggtaa aggcgtccaa gagctgtttg aaagtttagg
    54181 cgctaattat gttatcagtg gtggacaaac aatgaatcca agtacggaag acattgttaa
    54241 agcgattcaa gaagtaaacg cagataaagt gattgtcttg ccaaacaata agaacatctt
    54301 catggcagct gatcaagcag cagaagtagc agatatccca gtagcagttg ttccttcaag
    54361 aacgatttcc caaggattga cagctatgct ttcattcaat gatcaagctt ctttagaaga
    54421 aaacaaagaa acgatgacag aaatgttgtc tagcgttgtg agcggtcaag tcactcacgc
    54481 gatcagagat acctcaattg acggtgtaga gatcaaagaa ggagactttt tgggcatgat
    54541 cgatggaaaa atcctgatct ctaacccaga tatcacagca acagcgttaa gtacactaga
    54601 acaaatgatc aatgaggaca cagagatcgt aacgatcttg gcaggtgatg aaggaacagt
    54661 tgaacaagca aatgaatttg ctgagaaact aacagaaaaa catcctgatt tggaaatcga
    54721 gatccaccaa ggagatcagc ctgtctatcc ttatctattt tctgctgaat aatttctgtg
    54781 tcagaatatc tttctaacac agtattcatt cataaaaggc cttcggttag tgatcaggta
    54841 tgatcgctga ccaagtcctt tttttgttaa aggagggagg aaaaatttat gcgttcttta
    54901 gatgatccaa tcactatgct ggcaggagtc ggagaaaaaa gagcagctag tcttgccagc
    54961 ttgggcatcc aaacgattga agacttactg acttattatc cttttcgcta cgaagatatt
    55021 caggaaagaa atctaaacga gatccaggat caagaaaagg ttactttgaa aggaatcgta
    55081 gtctcgcctc ctgtgatgaa tcgatttggt tataaaaaaa gccgattgca atttcgtatg
    55141 atgcaagaac atgatgtgtt caacgtttct ttttttaatc aaccttatct aaaagaaaaa
    55201 gtcattttat cggaggaaat cgccgtatac ggtaaatggg atgcaaagcg taaagccttg
    55261 aacgggatga agattttagg ttctcaaaca atggatgact tttcgccgat atatcacgtc
    55321 aacaaatcaa ttcgtcaaac gacattagtt gatctaatca aaaaagggtt tcaagaatac
    55381 ggcacattga ttgaagaaaa tctgccaaac aaccttgtgg agaaatatcg actgcttgac
    55441 cgagcttctg ctgtaagaag tatgcatttt ccaaaagacc acgaagaaaa ccatcaagcc
    55501 aaacgacgtg tcgtttttga agagttcttt ttatttcaaa tgcgtcttca aggattgaaa
    55561 aaagcagaaa aagcagaaac gaatggtttg gagatccttt atgatgtcca aaaattgaaa
    55621 caatttactc aaaagctacc ttttgaactg acaaatgcac aaaaacgtgt aacgaatgaa
    55681 atctgtcggg atcttcgcag tccgcagcat atgcagcgac ttttgcaggg agatgtaggt
    55741 agcggaaaaa cagtggttgc cgcgattgcg ttatacgcta ccgttacagc aggtttccaa
    55801 ggtgcgttga tggtgcctac agaaatcttg gcacaacaac atatggaaag cttgcaacag
    55861 ctgtttgatc ccaatgaagt aaccatcgca ttgattacgg ggtcaacgaa agcaaaagaa
    55921 cgacgtgagc tccttgaaaa actagagcaa ggggaaatta acgtcgtgat cggcacacat
    55981 gcattgatcc aagatggagt cgaatttcag aatttaggtc tagttattac ggatgagcag
    56041 caccgttttg gcgtgaacca gcggaaagta cttcgtgaaa aaggctggcg accagatgtc
    56101 ttatttatga cagcgactcc gattcctaga acattagcta ttacagcata tggtgagatg
    56161 gatgtatcta tcattgatga acttccagct ggacgtatcc cgatcgaaac acgctgggta
    56221 agaacacctc aattggacag tgtactggat tggacgaaaa aagaactggc aagaggacat
    56281 caaatgtatg tcatctgtcc gctgattgaa gaatcagaag cattagatgt aaaaaatgca
    56341 gtggaaatct atgaaaaact tcgtgaacta tttgcaccaa aatatgaagt tggcttgtta
    56401 catggtaaaa tgaagaatca ggaaaaagaa gtaatcatgg aagccttcaa agacaaccag
    56461 atgcagatat tagtttcgac tactgtaatt gaagtagggg taaacgtgcc gaacgcgacc
    56521 gtcatgctga tcatggatgc ggatcgattt ggattggcac agctgcacca acttcgtgga
    56581 cgtgttggac gcggaagtga tgcttcctac tgtatcttag ttgctaatcc taaaaatgaa
    56641 ctaggtgtgg aacggatgaa aatcatgaca gagaccacca atggttttgt actaagcgaa
    56701 aaagatctgg aattacgtgg tcctggtgaa gtatttggct tccgtcaatc tggtctgcct
    56761 caatttgcga ttgctgactt ggtgacggat ggaaatgttt tggaggtagc cagagaagag
    56821 gctgcagccc tttggcaaat aaaggattgg cagatgcttc ctgagtatca gcctttagcg
    56881 caaagcctcc aaatagaaaa tcaggataat cgttatttcg attagcagac tgtgctatac
    56941 tttatactga tgacggaaaa aggagtggaa acaatgaaaa ttgccgtaga tgcaatgggc
    57001 ggagatcacg cacctcaagc aattgtagaa ggcgtggctt tagcgcagca agattttcca
    57061 gaagtagaat tcttacttta cggaaaagaa gcagaaatca agaaatactt aacaaatgaa
    57121 aaaaacatca cgattatcca tacagacgaa aaaatcaata gtgatgatga gccagtaaaa
    57181 gcaattcgac gaaaaaagac tgcatcaatg gtccttgctg cacaagcagt gaaaaacggt
    57241 gaagcagatg ctgtattttc agcagggaat acaggcgcgc tactagcagc agggttgttc
    57301 atcgtaggac gtatcaaaaa tatcgaacgc cctggcttga tgtctacctt gccagtagta
    57361 gggaaagaag gtgccggttt cgatatgctg gatcttggcg ctaacgcaga aaacaaacca
    57421 gaacatttgt tgcaatacgg tattttaggt tctttttatg cacgtaaagt ccgaggtatc
    57481 ggacgtcctc gtgtcgcact cttaaacaac ggaacagaag caacaaaagg tagtgaagtg
    57541 acgaaaaaag cctatgaact ccttcagaat gaaacaagct tgaattttat tggaaacgta
    57601 gaagctcgtg agctactcaa tggtgtggct gatgtagtgg tcacagatgg atttacaggc
    57661 aacgctgttt tgaaatctat tgaaggaaca gcaatgaata tgatgaatct attaaaatct
    57721 gctattctaa atgaagggat caaaggaaaa atgggcgcta tgcttttaaa agacggctta
    57781 cgttctttga aagcagaaat ggattattcc aaacatggcg gtgccgtttt gtttggcttg
    57841 aaagcaccag tcattaaaac tcatggagca acaggccccg atgctgttcg ctatacgatc
    57901 cgccaaatcc acacaatgct tgagacagat gtagttggac aattagtcga acaatttgaa
    57961 aaaagcgaag aatagtattt ggatatctga agcgatccgt tccattgaaa aaaaggtgga
    58021 cattcctgaa aaatgccagt aaaattagcc atggtgtagg taattgacaa gtgtatgatt
    58081 cttggaggtg aaaaagttga ctcgcgaaga agtatttaac aaagtagcga aaattatttc
    58141 aaatcatttt gagatagata ccgataaagt gacggatgaa ttgaacatca aagatgattt
    58201 aaaagctgat tcaattagta ttatggaatt cgttttagag cttgaagatg aattcggtac
    58261 ggaaatttca gatgaagacg ctgaacaaat tgaaacagtc ggtggcgcag tagattatat
    58321 cagcagccat cttaaataat ttcaaataaa agcaaagaag gataaaaagc tgcatgcagc
    58381 tttttatcct tcttttttta atctttttg
//
LOCUS       DANKHK010000005        55313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000005 DANKHK010000000
VERSION     DANKHK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            65..1513
                     /gene="cls"
                     /locus_tag="PW109_000261"
     CDS             65..1513
                     /gene="cls"
                     /locus_tag="PW109_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2510144.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            1850..2707
                     /locus_tag="PW109_000262"
     CDS             1850..2707
                     /locus_tag="PW109_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2510145.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            2822..3742
                     /gene="pstC"
                     /locus_tag="PW109_000263"
     CDS             2822..3742
                     /gene="pstC"
                     /locus_tag="PW109_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2510146.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            3742..4626
                     /gene="pstA"
                     /locus_tag="PW109_000264"
     CDS             3742..4626
                     /gene="pstA"
                     /locus_tag="PW109_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2510147.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            4641..5447
                     /gene="pstB"
                     /locus_tag="PW109_000265"
     CDS             4641..5447
                     /gene="pstB"
                     /locus_tag="PW109_000265"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2510148.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            5463..6221
                     /locus_tag="PW109_000266"
     CDS             5463..6221
                     /locus_tag="PW109_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2510149.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            6234..6911
                     /gene="phoU"
                     /locus_tag="PW109_000267"
     CDS             6234..6911
                     /gene="phoU"
                     /locus_tag="PW109_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2510150.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            7148..8179
                     /locus_tag="PW109_000268"
     CDS             7148..8179
                     /locus_tag="PW109_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2510151.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            8176..8943
                     /locus_tag="PW109_000269"
     CDS             8176..8943
                     /locus_tag="PW109_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2510152.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            9043..10611
                     /gene="liaX"
                     /locus_tag="PW109_000270"
     CDS             9043..10611
                     /gene="liaX"
                     /locus_tag="PW109_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2510153.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            10908..11228
                     /locus_tag="PW109_000271"
     CDS             10908..11228
                     /locus_tag="PW109_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2510154.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            11228..11578
                     /locus_tag="PW109_000272"
     CDS             11228..11578
                     /locus_tag="PW109_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2510155.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            11745..12683
                     /locus_tag="PW109_000273"
     CDS             11745..12683
                     /locus_tag="PW109_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2510156.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            12698..13528
                     /locus_tag="PW109_000274"
     CDS             12698..13528
                     /locus_tag="PW109_000274"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2510157.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            13572..14597
                     /locus_tag="PW109_000275"
     CDS             13572..14597
                     /locus_tag="PW109_000275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2510158.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            14612..15550
                     /gene="galU"
                     /locus_tag="PW109_000276"
     CDS             14612..15550
                     /gene="galU"
                     /locus_tag="PW109_000276"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2510159.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            15675..16115
                     /locus_tag="PW109_000277"
     CDS             15675..16115
                     /locus_tag="PW109_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2510160.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            16117..16719
                     /locus_tag="PW109_000278"
     CDS             16117..16719
                     /locus_tag="PW109_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2510161.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(16778..17884)
                     /locus_tag="PW109_000279"
     CDS             complement(16778..17884)
                     /locus_tag="PW109_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2510162.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKILP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            18094..19095
                     /gene="ccpA"
                     /locus_tag="PW109_000280"
     CDS             18094..19095
                     /gene="ccpA"
                     /locus_tag="PW109_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2510163.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(19432..21777)
                     /locus_tag="PW109_000281"
     CDS             complement(19432..21777)
                     /locus_tag="PW109_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2510164.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            22056..22958
                     /locus_tag="PW109_000282"
     CDS             22056..22958
                     /locus_tag="PW109_000282"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2510165.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTTQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            23171..24946
                     /locus_tag="PW109_000283"
     CDS             23171..24946
                     /locus_tag="PW109_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2510166.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(25012..26598)
                     /locus_tag="PW109_000284"
     CDS             complement(25012..26598)
                     /locus_tag="PW109_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2510167.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            26849..27073
                     /locus_tag="PW109_000285"
     CDS             26849..27073
                     /locus_tag="PW109_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2510168.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27215..28966
                     /locus_tag="PW109_000286"
     CDS             27215..28966
                     /locus_tag="PW109_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2510169.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            28966..30750
                     /locus_tag="PW109_000287"
     CDS             28966..30750
                     /locus_tag="PW109_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510170.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVECGNHETLLAQNGLYADMYKLQSSGV"
     gene            31208..32059
                     /locus_tag="PW109_000288"
     CDS             31208..32059
                     /locus_tag="PW109_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2510171.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQISTDEKMYLTIHTARL
                     VHENRCK"
     gene            32156..34003
                     /locus_tag="PW109_000289"
                     /pseudo
     CDS             32156..34003
                     /locus_tag="PW109_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            complement(34044..34946)
                     /locus_tag="PW109_000290"
     CDS             complement(34044..34946)
                     /locus_tag="PW109_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2510172.1"
                     /translation="MLLREKIKQISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLDETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            35176..36579
                     /locus_tag="PW109_000291"
     CDS             35176..36579
                     /locus_tag="PW109_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2510173.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36576..38012
                     /locus_tag="PW109_000292"
     CDS             36576..38012
                     /locus_tag="PW109_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2510174.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDEDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38157..39083
                     /gene="trxB"
                     /locus_tag="PW109_000293"
     CDS             38157..39083
                     /gene="trxB"
                     /locus_tag="PW109_000293"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2510175.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39297..41024
                     /locus_tag="PW109_000294"
     CDS             39297..41024
                     /locus_tag="PW109_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2510176.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            41039..41389
                     /locus_tag="PW109_000295"
     CDS             41039..41389
                     /locus_tag="PW109_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2510177.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41481..42341)
                     /locus_tag="PW109_000296"
     CDS             complement(41481..42341)
                     /locus_tag="PW109_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2510178.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            42433..42861
                     /locus_tag="PW109_000297"
     CDS             42433..42861
                     /locus_tag="PW109_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2510179.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43023..43199
                     /locus_tag="PW109_000298"
     CDS             43023..43199
                     /locus_tag="PW109_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2510180.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43226..43672
                     /locus_tag="PW109_000299"
     CDS             43226..43672
                     /locus_tag="PW109_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2510181.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43826..45205)
                     /gene="brnQ"
                     /locus_tag="PW109_000300"
     CDS             complement(43826..45205)
                     /gene="brnQ"
                     /locus_tag="PW109_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2510182.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            45911..46882
                     /locus_tag="PW109_000301"
     CDS             45911..46882
                     /locus_tag="PW109_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2510183.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            46906..49098
                     /locus_tag="PW109_000302"
     CDS             46906..49098
                     /locus_tag="PW109_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2510184.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            49115..49591
                     /gene="ybeY"
                     /locus_tag="PW109_000303"
     CDS             49115..49591
                     /gene="ybeY"
                     /locus_tag="PW109_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2510185.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49575..49970
                     /locus_tag="PW109_000304"
     CDS             49575..49970
                     /locus_tag="PW109_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2510186.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            49985..50884
                     /gene="era"
                     /locus_tag="PW109_000305"
     CDS             49985..50884
                     /gene="era"
                     /locus_tag="PW109_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2510187.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51027..51839
                     /gene="recO"
                     /locus_tag="PW109_000306"
     CDS             51027..51839
                     /gene="recO"
                     /locus_tag="PW109_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2510188.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52223..53140
                     /gene="glyQ"
                     /locus_tag="PW109_000307"
     CDS             52223..53140
                     /gene="glyQ"
                     /locus_tag="PW109_000307"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2510189.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            53142..55217
                     /locus_tag="PW109_000308"
     CDS             53142..55217
                     /locus_tag="PW109_000308"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2510190.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
BASE COUNT        17939 a         9761 c        11781 g        15832 t
ORIGIN      
        1 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagag gaagaggtga
       61 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
      121 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
      181 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
      241 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
      301 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
      361 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
      421 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
      481 caagcaagca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
      541 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
      601 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
      661 aggcggagaa atcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
      721 ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa atcggatata caggtgggtt
      781 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
      841 tttgcgtatt tacggagaag cggtgtatat cttgcaaaac cgttttttga tggactggaa
      901 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcactctac
      961 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
     1021 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
     1081 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
     1141 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
     1201 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
     1261 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
     1321 tgttcgttca tttcgcttgg actttgaggt caatacactg atttatgatg aacgaatggc
     1381 ttcacgtgta agaaaagcat tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
     1441 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
     1501 gccgctcctt taaaacacaa aaaggacatg agaaccatca acagatggag atctcgtgtc
     1561 cttttctaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
     1621 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
     1681 gctattaaaa gagacacatt tcaaagccag tatagcatag aaacttttaa tgagatagtt
     1741 ttttttacaa ttatgaggat ttattttgtt atttaatgat tttgattcgt tttcatcaag
     1801 taatgaaaat gataaaataa acatacttta ggattaaagg agatttctaa tgaaaaaact
     1861 taaattattc ttaccaataa taggattagc tattctttta tcaggctgtt caaagtggat
     1921 tgatcgagga gaatccatta cggctgttgg ctcttctgct ttacagcctt tagtagaaac
     1981 agttgctgaa acttaccaga cagaaaatcc tggaaaattc atcaatgtac aagggggcgg
     2041 aagcggaact ggtttgtctc aagtacagtc aggagcagtc gacatcggaa actctgattt
     2101 gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc gagtagcagt
     2161 tgttgggatc actcctattg tgaacaagga tattggcgta aaagacatca gtatggaaaa
     2221 cctgaaaaaa ctatttacag gcgagatcaa aaactggagt gaagtcggtg gaaaagatca
     2281 gccagtagtt atattgaacc gtgcttcagg aagcggaacc cgtagcacgt ttgaaaaatg
     2341 ggtattggac ggagaaacag ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg
     2401 ccagatcgtt gccgacacac ccggtgcaat cagctatgtt gctttttctt atgtttccga
     2461 tgatgtagat acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg tgacaacgaa
     2521 caaatggacg atttgggcat atgagcatat gtacacaaga gatcatccaa aaaaactgac
     2581 aaaagaattt ttagactata tcttatctga tgaagtacaa gataacatcg ttggagaact
     2641 aggttatatc cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa atatcgttaa
     2701 agagtaatct ttacacaaaa tttacaaact tagctaaact tttacatgtt attaaaacca
     2761 aattgatgtt caatttgtac actgagtgag tatgttaaac ctgattaagg gggagtcatc
     2821 tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg
     2881 tggaaaattc atcagttttt tatgtatcgc gctgatcgtg ttggttgtat tatctatttt
     2941 ttattttgta gctagtaaag gattggccac cttttttact gataagatca atgtatttga
     3001 ttttctgttt ggtacagatt ggaatccaag ccaaatcgga gcaaatggga aaccaatggt
     3061 aggggcattg cctatgatca caggttcgtt tattgtgacc tttttatctg ctattgttgc
     3121 cacaccattt gccattggtg cagcagtatt catgacggag atctctccta aaaacggaac
     3181 gaagatctta cagccggtca tcgagctgct tgtaggtatt ccttctgtcg tttatggttt
     3241 tatcggttta tcagttatcg tgccttttgt cagaagtatt tttggcggga cagggtttgg
     3301 tatcttagct ggtacatttg tgttgttcgt tatgatcttg ccgactgtca caacgatgac
     3361 tgttgatgca ttaaaggcag taccacggca ctatcgggaa gcttcattag cgcttggtgc
     3421 cacaagatgg caaacaatct acaaagtcgt gctacgagca gccgtgcccg ggattttaac
     3481 agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc aaatggtcat
     3541 cggtaatgca gcattgatgc caacaggact tactactcct gcttctacat tgacgtccat
     3601 cttaacaatg ggaatcggaa atacaatcat gggaacagtt gaaaataatg tgttatggtc
     3661 attagcgctg atcttgctat tgatgtcatt attctttaat atcgtgatcc gtatgattgg
     3721 gaagaaagga gagctgaaat aatgaacgca aaacgctcag ataaaattgc tacaggtgtt
     3781 ttatatgtag tttcggggat catcgtattg attttagcag ctttactact ttatatctta
     3841 gttcggggaa taccgcacat ttcatggtcc ttcttgaccg aaccttccaa agcctaccaa
     3901 gaaggcggtg gaatcgggat ccagctattt aactcgattt atttgctttt gatcacgatg
     3961 atcatcagtt ttcctatttc tttaggtgcg ggtatctatc tttcagaata tgctggaaaa
     4021 aactggctga cagatgtgat tcgcacgtcg attgaaatct tgagctcatt gccttctgtc
     4081 gttgtcggtt tattcggttt cttggttttc gttattcaat tccagtatgg tttctcgatt
     4141 atttctggtg cattggcttt gactgtattc aacttgcctc ttttgactag aacagttgag
     4201 gagtctttac aagcggtcca ttacacacaa cgagaagcag gattggcttt aggtttgtca
     4261 cgctgggaaa cagtgataaa agtggtggtt ccagaagcga caccagggat tttgactggt
     4321 gtgatcctaa gttctggaag aatttttggt gaagcagctg cattgatcta tacggctgga
     4381 caaagtgcac ctgcgttaga ttttacgaac tggaatccat taagtgtttc aagcccgatc
     4441 agcattttcc gtcaagctga aacattagct gtccatattt ggaagatcaa tacagaaggc
     4501 acgatgcctg atggtgtttc tgtttcggct ggcgcttctg ccgtgttgat cattgctgtt
     4561 ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca
     4621 gcctaaggag aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat gtcatcacca
     4681 tgccaaaaga gaaagaagta gcactatata cggatgatct acatgtctgg tatggcgaca
     4741 atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa taagatcact gcactgattg
     4801 gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg aatgatggga
     4861 tcgctaatac gaaaatcact gggaaaatca tgtatcaagg cgtagatatt aatgcgcctc
     4921 aagtggatgt ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt cctaatccgt
     4981 tcagcaagtc gatttatgac aatattacat ttgctttgaa acagcacggc gaaaaaaaca
     5041 aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca agctgccttg tgggaccaag
     5101 taaaagacag cttggacaaa agtgccttgg ctttatctgg cgggcaacag caacgtttat
     5161 gtattgctcg tgcgatcgcg atgaaacctg atatcttgct gctagacgag ccggcaagtg
     5221 ctttggaccc tatttcgaca ggaacggttg aagaaacact tgtaaacttg aaagaagact
     5281 atacgatcat catcgtgacg cataacatgc aacaagcggc tcgtatcagc gactatactg
     5341 cctttttcta tctaggaaaa gtaatggaat atgacaaaac taacaagata tttaccagac
     5401 caaaaattca ggcaaccgaa gattatgtgt ccggtcattt tggttaagac ggaggcctca
     5461 acatgacaaa agaaattatt tcatctaaag atcttcatct gtattatgga aagaaagaag
     5521 cactaaaagg gatcgatcta agtttcaatc aaggggaaat cactgcgatg atcggtcctt
     5581 ctggttgcgg gaaatccact tatctgcgtt ctttgaaccg gatgaatgac ctgattccga
     5641 atgtaacgat caccgggagt gttttatata aagacaaaga catctatggt ccaaaaacag
     5701 atgttgttga actaagaaaa gaaatcggca tggtattcca gcagccaaac ccatttcctt
     5761 tttctatcta tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa gataaaaaag
     5821 tccttgatca agtagttgaa gaaagcctga aggcagcgtc tgtttgggat gatgtgaaag
     5881 acaaattgca taaaagtgct ttatctttat ccggtgggca gcagcagcgt gtatgtatcg
     5941 cacgtgtttt ggcagttaat ccggaaatca tcttattgga tgaaccaaca agtgcgcttg
     6001 atcctgtttc tactgggaaa atcgaaagta tgctgttaga attaagagag cggtatacga
     6061 tgatcatcgt gacccataat atgtctcaag cttcacgtat ttcagataag acggcattct
     6121 ttttagatgg tcacttgatc gaatacaatg atacgaagaa gatattcatg aatccagaaa
     6181 aacaagagac agaagattat atttcaggtc gatttggata aggaggaaac ggaatgctac
     6241 gtacacaatt tgaagaagaa ttgctgaatc tccataatca gttctacgaa atggggatga
     6301 tggtgagcag cgccgtgcac aagtcagttc gtgcatacat caaacacgat aaaatcttag
     6361 cccaagaagt tatcgaaaac gatataaata tcaataacat ggaaacacgc ttggagaaga
     6421 aaagttttga aatgatcgca ttgcaacagc cagttacaac tgatttgcgt atgatcatta
     6481 cggtcatgaa agctagctcg gatttagaac gtatggccga tcatgcggtg tctgttgcta
     6541 aatccacgat tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa gagatctccg
     6601 atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat gtcaaaacag
     6661 acgaaaaaga cgcacgtacg atcgccaata tggatcaaag agtcaatgac tatttcaatc
     6721 gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc atcagtggaa
     6781 cagactactt gaatgttgcc agttatctag aacggatcgg agactatgtg acaaacatct
     6841 gtgaatggat cgtctacttg gctacaggga aaatcactga gttaaatacg aaccacaatg
     6901 agattttcta aactaaagcg gtgattctgc cgtttgttta ggaaaagagg ttgtgaaatc
     6961 agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca aaaatagctc
     7021 ctcatatttt tcatttggct ggacttatta ccgcagatgc aagcttttga acactgttta
     7081 ttcgggaaag ggtttgtgac aaaatatgtc acaaaccctt ttcatcattt catagaaagg
     7141 ataaagaatg caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc
     7201 atgggttatt cagcatcaag ctgctttata tgaagtacct gtcatcgaga tcgaaaaagt
     7261 acgaataaaa gaggatcatc aattgattac aggaagactg atgaacacga atcaaaaagg
     7321 agaatcgatt tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa
     7381 agcgagacaa atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa
     7441 aaatgatggc tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg
     7501 cggaaaacat ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact
     7561 tttgttgctc atctttacta aaatagaagg gatttctcta ttaaatatat ctatcctgtt
     7621 cgtcgttata gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat
     7681 aatcaaaatt tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac
     7741 aatgaatctt tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc
     7801 gtatcgttct gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac
     7861 cgttatcaca gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc
     7921 gaaacagtta tggctttcgg gaaggcaagt gggtcgagat gtgattggct cgatgaccaa
     7981 tatccttttg ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa
     8041 tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat
     8101 tgctggcagc ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt
     8161 aaggaggctg aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg
     8221 ctacttattg gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg
     8281 atctttttct tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc
     8341 ataggatttt tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa
     8401 atgcgtgcag cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct
     8461 atcactgatt ttttagcaat ccaaggtttt acaacgatag aactagaaga attatctggc
     8521 ttagataaga ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt
     8581 cttagtggtg ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat
     8641 caggcaaatc cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa
     8701 aaaatattgg cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg
     8761 ttgatcctct ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca
     8821 tttgttctag aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct
     8881 ttaacctgcg tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag
     8941 taaaagttac aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta
     9001 aggaaaagct tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt
     9061 attttagatt tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa
     9121 agcatggcaa ctgaaaaaga cgaaaagcaa ataaaaaaag aagcagacaa ggtaactgct
     9181 agtcataaag aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa
     9241 gaaatttctg agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa
     9301 aagatcttag atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta
     9361 aatgctcaat tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag
     9421 ctgaatacaa aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg
     9481 ttagaagcag aaatcaaaga gttagaagct agtggcgata cactgttaga agaacaattg
     9541 aaattagaag cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg
     9601 tttactgata agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat
     9661 caagtaggcg aaaaaatgtc agaagctgga tcacagttag gaaaattctt gaagaaaaca
     9721 ttccaaactg tttcagaaac agtgaatgat aacatggaat ggaaagatgt cagcttacgt
     9781 gtccctggga ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca
     9841 agtatccttg atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac
     9901 gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa
     9961 gcattctctg aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca
    10021 aataaacgtg tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca
    10081 gctattaaac ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat
    10141 acaaaatcta cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta
    10201 gaaggtgtaa acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa
    10261 acagtaaatg gtacagtgac atttggtgcg acagcagaaa atctttctgt gtctttagta
    10321 aatggtgatg tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca
    10381 gtgaacggaa atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa
    10441 acaagtcttg gaagtatcaa tagccgtctg tccaattacg aagtagttcg tgagaaaaaa
    10501 gaacggacaa accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg
    10561 caactttcaa cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt
    10621 gaaagagatg ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt
    10681 tacaaaattg tgccttattg ccgataggct atctcttgaa caccgtttac caagagatta
    10741 tgaacttgcc acttatcgcc gagtcacaag gaataatttc aaaaaaagct cgtcacgttt
    10801 tatgaatatg gtaatttgtg accgagaatt ttgcagagag acaccgatta agaagaaggt
    10861 tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa
    10921 agttgacaaa atccaataaa aatgttgttt tgacagggac tttagcagga ataggagaat
    10981 acatcgggat tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt
    11041 ttggaagtcc aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg
    11101 cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa
    11161 ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg
    11221 acttttaatg tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc
    11281 tgttcttttt cctgcgatgg tccatgtaag cagtatttgg acggccgttc ttgccagctt
    11341 cgtattgtct gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt
    11401 tacattagtc acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac
    11461 ctcctttttt gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt
    11521 agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa
    11581 gaaagctgtg ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta
    11641 ttacattggt aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat
    11701 gataaaatga tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa
    11761 aagttagcca attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct
    11821 tagaccgtgt catcgtaact ggggacatct cacgcccagg actagagttg actggttatt
    11881 ttaattatta ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg
    11941 aacggatgat cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc
    12001 ccgcatttat tatttcaaga ggattaccgg ctccagaaga aatgatccag gcagcaaatg
    12061 aaaatgggat agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat
    12121 ccagttattt agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg
    12181 tttacggatt aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt
    12241 tggagttgat caaacgcggt catcgattga tcgcagacga tcgagtagat gtttatcagc
    12301 aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct ggaacatcta attgaaatcc
    12361 gaggaatcgg gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta
    12421 tgcaagtcca gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc
    12481 tgggctctga agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc
    12541 ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg
    12601 cacgtacaat gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg
    12661 aagaaaactc aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt
    12721 tgcttttgag cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat
    12781 cattatcgcc atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga
    12841 tgtgacagat tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta
    12901 tgttttattt gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc
    12961 aggaggctta gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac
    13021 taaatatcat tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt
    13081 agcacaggca atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac
    13141 gacgcgaagc ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat
    13201 cgaagggatc tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt
    13261 tattttgctt ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt
    13321 atgttatcta atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag
    13381 tctttacttt ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg
    13441 tacattgatt ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc
    13501 attaggaaaa aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca
    13561 ggagtggtaa aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt
    13621 tggcacaagt cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac
    13681 aagtagaaga aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac
    13741 ccgattccat ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc
    13801 tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga
    13861 atacaaagcc agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga
    13921 tctctgacat cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt
    13981 ctggaccaag ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa
    14041 gtacagatgc aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga
    14101 tctataccaa tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg
    14161 ctattggtgc tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca
    14221 tgacgcgcgg attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgttga
    14281 cctttatcgg attaagcgga gtaggcgatc tagtcgttac ttgcacaagt gttcattcta
    14341 ggaactggcg cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca
    14401 atatgggaat ggtcgttgaa ggtgtgaata ctactcaagc agcgatggaa ctagcccaaa
    14461 taatggaaat cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag
    14521 aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg gaaaaaagaa aatgaattct
    14581 cattaacaat tttttgaagg agcgaatgta tatgaaagtc aaaaaagcag ttattccagc
    14641 agcaggatta ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc
    14701 aattgttgat aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggaatcga
    14761 agacatcttg atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa
    14821 tattgaatta gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga
    14881 agaaacgaca gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca
    14941 tgccgttctt caagcaagag cctttattgg aaatgaaccg tttgtcgtta tgctaggcga
    15001 tgatatcatg gaagatgaag taccattgac acagcaatta atcgatgatt acgaacgtac
    15061 tcatgcttct acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat
    15121 tatcaatcca ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa
    15181 accagatcca agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc
    15241 agaaattttt gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac
    15301 tgacgcaatc gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa
    15361 acgttacgat gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac
    15421 acatccagaa gtaggtgcac ctttacgtga gtatatcctg tctttaggta aaacattaga
    15481 agcagaagga agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa
    15541 gaaaaaataa cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa
    15601 gctcgttttt atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag
    15661 aagggagttt gatcatgtca ggaggagaaa ttgcaggatt aatcgcagca atcgcctttg
    15721 cgatacttgt cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag
    15781 tagatcagac tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc
    15841 ttttgtctag acaagtagaa ggcttgctgg tcaaaagtaa tgaactgctc ttggatatca
    15901 atggaaaagt cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg
    15961 tatcagaact taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa
    16021 cgacagctaa agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa
    16081 acaaaaaaca tactgaaacg acaggaggaa aataaaatgg ctaaaaaagg tggattttta
    16141 ttaggtgcag tagtcggagg aacagccgca gcaatcgccg cgttattgtt agcaccaaaa
    16201 tctggtaaag aacttcggga tgaattagct gcccaagcgg atgatttgat ggatatggct
    16261 tcagattaca cagatatggc gaaagaaaaa tcgactgagt taacaggtat tgcaaaagaa
    16321 aaagcgcaag atctttctca acaagcaggt gatctagcag gtagtgtcaa acaaaaagta
    16381 agtgaatcag cagatgaagc aggaagtgaa tctgacgata tcttgttaaa tcttaaagaa
    16441 caatcagctg acttatctaa tcgtttcaaa aaaacggcag aagatatgaa aaaacctgca
    16501 gatgaattag gaagtattgc taaagatact tcagaagata tcatgattga tgtaaaagac
    16561 gctgctggta aagcaggaga tactgtcaat gaaggaatgt cagaagcaaa accagttgca
    16621 gatgacacaa aagaaatgat gaaaatggca aaagaagatg cgaaagaagc taaaaaagaa
    16681 ataaaagata caatgacaga ttcatctaca aatcactaaa atcacaaatt tatagaataa
    16741 aaaggtggga gcggactagc tctcaccttt tttactctta tttttctaaa acaagtaatt
    16801 cttttggtgt agtcgtgaat ggcacacagc catcgcttgt cacatataca cagtcttcga
    16861 tacgtacccc tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt
    16921 cttcgattac taagtcattt ccttctacaa gggaagggaa ttcgtggacg gttgttccga
    16981 tcccgtgacc taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc
    17041 gtgcgatttg atcaagttca cctgctgtca cacctggacg gacggcttct tgtgcggcaa
    17101 gctgtgcttc aagagtgatg ttgtatattt tttcttggaa gtcggttggt ttttgataag
    17161 caattgtacg agtagcatca ctgcaatagc cattccaaac aacgccaaga tcgaataaca
    17221 caagttcatt cggtaaaatc ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg
    17281 ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac accttgtttt ttcaattggt
    17341 attcgatttc tgcaatgatc gcttgttcag tcacgccttc tttcactgct ttaaagccaa
    17401 tctcaaatgc aacatcggcc catttaccag cttcgatcag tttgtctttt tcctctagag
    17461 ttttgatcag ttgtagacgt tggatgatgg aagttagatc agtgtaagaa tccgttcccg
    17521 tcaagatatc agagatagca tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta
    17581 cattgccagg ctgaccatag cgagcacgaa gttcttttcc gatgatctcc caagggtttt
    17641 cgctgtctaa atagccacgg acatcatacg cccaactact gttttgagca tcttcaactt
    17701 ccaaagcagg agtgaaaagg aaaggatctt gttcaagagg gataaaaaga gctagtacac
    17761 gctcatgagg atcactttcg tatccggaaa agtaagcgat gtggccaggg tcgctgatat
    17821 aagccaattc gacttggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat
    17881 tcatttggca acctactttc taaattaatt ttgtattcaa taattgtacc actgatttat
    17941 taaaagacca acattctaaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa
    18001 acggttgcaa ttcttgagat tatttgatat tctattttag aaatgtaaga cgtattttca
    18061 gaaaacatat aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat
    18121 gatgtagcga gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat
    18181 ccaaacgtta aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat
    18241 cgtccaaacg cagttgcgcg tggcttggca agtaagaaaa caacaacagt tggtgtaatc
    18301 atccctgatg tcagcaatat gttctttgct tctttagcac gtggtatcga tgatgtggca
    18361 acaatgtaca aatacaatat tatcttagca aattcagatg gcaacgacca aaaagaagtc
    18421 aatgtattga ataacttact ggctaagcaa gtggacggcg tgatcttcat gggtcaccat
    18481 atcacagacg aaatccgtgg agaattttca cgttcaaaaa ctcctgttgt attagctggc
    18541 tctattgatc cagatgaaca agtaggtagc gtaaacattg attatgtatc cgcaacaaaa
    18601 gatgcagtaa acaaattggc aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg
    18661 ttgatcgatc ccatcaatgg acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa
    18721 aataacctac catatagcga aggattgatc tttgaagcac aatacaactt caaagatggt
    18781 ctatctctag tcgatcggat ccataatagc ggtgcaacag ctgtctatgt ttcagatgat
    18841 gaattagcca tcggtatttt ggatggttta ttagaccgtg gcgtgaaagt accggaagaa
    18901 ttcgaaatca tcacaagtaa caattcacta ctgacagaag tagctcgtcc tcgtttaaca
    18961 agtatcacac aaccattata cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg
    19021 atgaataaag aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa
    19081 ggatctacaa aataataaaa ggacaaccat tcagaggttg tgtgcctgcc gtttaactct
    19141 gcgttacaag gagcaatttc aaaaaacagc atctcatgtt tgatgaatat tgtgactcgt
    19201 gattcggagt tggcaggaga acaccgttta tttgggaaaa ggggttatga caaaattttg
    19261 ttataatcct ttttttgtgt cttaaaaaag agagaaagtg gttgaaatta tatttatcgt
    19321 atataaaaca atccgtgtat agatgctgta ttttgttcac catcatcaaa aaaataaggg
    19381 aatgtgacaa tactattgtc acattccctt attactgtgt attctattaa atcaatcatc
    19441 tttctcatct gattctttgg ttgcttgatt cgctcttgca taagctgaag cggtgttcca
    19501 gtaatctgta tagttttcat cggtaccgcc aacgccaaac ttaaaggcgc ttttcttcgg
    19561 tccatttttc gcccatagcg attcaactgt ttttcctggt gtttggatgg attttttatt
    19621 gacagtaact tttccaggtg tttgaccagt aaatccagat acttttttct tcatcacatc
    19681 agatgataat tcgaatttct catcagtgcc aaaaatgtca ggttttgctt ggtaagcttg
    19741 attgatcaga ttggcaagat aattagccga acgtatcctt gctttgctgt ccattccagt
    19801 attgtcatcg tgccctgccc agctactgat ggtaatagac ggagtagaga caattagcca
    19861 tgaatctcgg tattcattgg tagaaccagt ttttccgacc caatctgctt ttcctagatt
    19921 tccgtttaag cttgagatgg catctttgaa tggtgtagtg atttttgcat tgatcacact
    19981 gcgcatcatg tcattcatga tcgatgccgt tgcttccgaa tagatccgga cagagttgct
    20041 ttcgtgttta taaattacat ttcctgcgtt atctgtgatg gaatcgatga tatagccttc
    20101 ttcatagacg ccgccgtttg ctaaagcctg aaatccgttg gtctgttgaa gcgtcgtgac
    20161 atctactgta cctaaaggag cagattcaac gccccaattg tcatttgccg ggtagttcat
    20221 ctttgacaag tagtgctcgt aagcatattg tttcgatcca ccggaattca acacatcttg
    20281 atataagtga taagccggga tattgttgga ccattctagt gactcacgga cggtttggaa
    20341 ggtgttcgat ccttcgttag tagcattgac gattttctcg ccagcatttt ctccttcttg
    20401 ccaagttgtt gcataatctg atacacggga ttctgatccg ataagcccca tgtcgattgc
    20461 aggtccgtaa acaagaactg gtttgattga tgagccagct tgacgttctg tatcgaaggc
    20521 atggttattt tgattctgac tgtaattccg tccgccaacg aatccgtaga tgcggccagt
    20581 tctgttatcc atcaatacat ttccggtttc tacttgactg ccagcaccat catctaacat
    20641 gtatccataa ttttcaacgc cattttgcaa tgcagcatag acatctttat cgattgtcga
    20701 atggattgta tatcctttat tttggatcgt ttgctgtgcc ttttgataat aagcgtcact
    20761 gatcgatgaa tcagacatat cagcgttgtc tttttcagcc aattgttgtg caatgattct
    20821 tgttgcttca ttcatcacag tataatacaa gaattctctg tcagtctgtt ctgcgatttg
    20881 ttgtggtaga aagtcttttg taaggtcata ggcttttgct tcttcgtatt gttctttcgt
    20941 aatttggcct tcacgataca tactgaataa aacaaaatct tttctggcaa ggccggcaga
    21001 aagatcttct tttagcgcac ctgtgttcgt gtatggacta tacgtgatcg ggctttgggg
    21061 taatccagcg atataagcag cttgcggcag agtaacatct tttgcatcta caccaaagat
    21121 tcctcttgca gcttcctgga ctccagcaat gttttgtccc ttattattac gaccaaatgg
    21181 tgagacattc aaatacgtag caatgatctc atcttttgaa aagtattttt ctacttgtgc
    21241 agaaagaatg atctcgtttg ctttacgttt gaatgttgtt tcatctgtaa ggatctgctg
    21301 tttgactaat tgctgtgtca aagttgatcc accagaagag ccaataccag tagcttcaga
    21361 tactaatgct cgtaaaacag ctttaggaac gtaaccttgg tgtttatcga agtattcatc
    21421 ctctgtgctg ataattgcag ttttcaataa aggtgaaatt ttatcagatg aaatagtcgt
    21481 gcgcatcaga tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga
    21541 cgtttctgta atgtttccta gttctgtctg cagtgctttt ttagtcggca attgtgtgtc
    21601 ttcgacgaga taggcaaaat agccagcgcc tattccaata cctagagctg cgaacatcaa
    21661 aagaagaaca gtaataaaaa cagtcaatga ttgaaaaact ctaaaaataa tcttaggaac
    21721 aaaccatttt tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag
    21781 ggtcacctca actaatggtt ttatttcgct aattatagca aataatagaa caaaaaaaca
    21841 gacaaggctg tttttggcag gaatttcttt tttaaaggag ttaaaacgtg agaaaatgat
    21901 cttttttata aaagagttct taagcttttc tttagagaaa atttcagtta ctatggagaa
    21961 cagggcaaag aaaaatcaat cattttcttt gtatatcatc ttaccaatat ataggaattg
    22021 ttagtaacaa ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca
    22081 tgagcggaaa gaaaatgctg ttaggagcag cagcagaagc agccagttat ggatcttcga
    22141 cttttatgat ctatacaggc gcgcctcaaa acactagaag aaaagccatc gaagagatga
    22201 atattgatgc cggaaaagcg tacatggcag aaaacggtct aacaaatatc gtcgttcatg
    22261 ccccttatat cattaatttg gggaatacga tcaaaccaga aatgtttcct tttgccgtcc
    22321 aatttctgag agaagagatt gttcgtgcgg aagctttagg cgctactcaa attacacttc
    22381 acccaggtgc tcatgtaggg gcaggaacag aagccggaat tgctagaatt attgaaggct
    22441 tgaatgaagt tttgacaaaa gaacagacga cacaaatcgc tttggagaca atggcaggta
    22501 aaggtacgga aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat
    22561 taaatgaaaa attatccgtt acttttgaca cctgtcatac gaatgatgcc ggttataata
    22621 ttaaagaaga cttttcaggt gtaatggaag agtttgataa gatcatcggg ttagatcggc
    22681 ttaaagtact ccatatcaat gattctaaaa atccgcaagg ttcgcataaa gaccggcacg
    22741 ccaatattgg ttttggaaca atcggattcg acacgctgaa taagatcgtc catgatccaa
    22801 tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt cggcaaagat aaaaagaaag
    22861 cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc acaaatattc gatccagaag
    22921 tgtttcctga gttattagca gaagaagtga tactctaaga ttttctatct gacagcagca
    22981 aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa gaaacaaagg agtacttttt
    23041 gcagtctttt tcatcatgca tcttctcccc cctcttttta aatttagaca aacaaggtat
    23101 actgttagaa aagtaaaaaa acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa
    23161 ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac caactaaaaa agaaacatac
    23221 atactttatt cttggcatag ccctattatt tacaattatt cgtttgtatt tatatagtaa
    23281 agcaatctat ggaacagaca tgtcggcaac ctatgatgac cagctgctga ttcgctatgc
    23341 tcaaaatatt ttggacggta aatggctagg ggagtatacg actacaacac tttccaaagg
    23401 aatctcctac agcttatttt tagtacttgc acataaactg catctttctt atgctgtttt
    23461 gttcggcatg ttcagtattt ttgccagtat attggttgca ctcgcgttta aaccgatcgt
    23521 gaagaacaag tatcttcttt taagtatcta tttttatttt ttgttttctc cgattagttt
    23581 tacacatgaa tattccactc gaacatatcg taatgccctc gtgattccgg ctgttgagat
    23641 cattatcgct tgtttactag ctatttatta ccgcaaatac ggttctagca gaaaattgct
    23701 gccatggttt ttagggcttt ctgtcgcttt tcctttcttt tggttcattc gcgaagattc
    23761 tatctggctt cttcctttta ctgtagtagc tttgggtatc agtttcgtcc agatcttgct
    23821 agggcaagac ttttctttca aagaagtaaa gaagttaaaa aaacgactct ttcttctctc
    23881 gaaacgggaa tggctgaaac tttcattgtt tttacttcct atagttggct tttccatcgt
    23941 ctatcagtgg atcgaatata aaaatgagac aacatacggt attgcaacca tcaatgaccg
    24001 ttccagtgga gaatttggac gtttgatgaa acaactgatc cgaatagatg atgaaacgaa
    24061 cttgaataaa gaaaacagtg agatatgggt ctcacatgct gcactaaata aagcattagc
    24121 cgcatctcct acattccata ctgtttctac ccgtattgac gaattatata cgactcacgc
    24181 gtggactcaa ggtggaaaag taaaagaatt acccggagat atcatctttt gggcattgcg
    24241 agatgtggta agcgaatctg gttattacca aaacaatgca aaagaaacca atgatttttg
    24301 gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag ggaaccatca aaaagaaaaa
    24361 agagttctat ctgatgggat caggagatgg aaagacgatg agtgatctgc caatcgtatt
    24421 cgatttcctt caatcaggat acgcttatgc atggcgatat aaagatttcc atcaaggagg
    24481 aaattatagt ttcggaacgc aagaacaagt caaacaggct caagaattat taaaccttcc
    24541 gctaatggag aattggatcg atagtaatga accgagaaca cctaatttcc aattaacaaa
    24601 atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa gttctggcta ttcccgtgct
    24661 gattgcttca atagcaggga tattacttat gatactagga accgtatttg caaaagctga
    24721 tcgtgcctat tatagttcct ctttgattgt ggtcaccgga ttaatactga cacaattggt
    24781 ttttcttttc ggtgtctcgt ggttttgttc ttatgcgcca gaaaaaaagg attatttttt
    24841 aagtgtctac actggcgcag gcgtgccatt aacgcaattc atcgtagtaa caagcatgct
    24901 agttttcagt aaattaaatc tgaaaaaggc tttatcttct aaataaaaag acccttggta
    24961 gctagatttt taacctaact atcaagggtc tttgtgtgga aaaatcagtt tctattcgat
    25021 ttcttctacc gtttcctgca aacgctgttc atattttttg tttcctttat ataaatcacc
    25081 aatcgtccat gccaacagga gaaggacaag aacaatgatt acataagcga tgtgatcagc
    25141 caatgctagt tgagcagaag tggcttgttc accaaagaaa ctttcgatct gtcctggtaa
    25201 tcctcgtaag ttcaagtaag ttagactaat cacagagaac cagccaagaa gttttacgat
    25261 gaatgaattt ttaaatcggc tacccatctc cgcttggctg ttggtcatca tcagtaaagg
    25321 gatcatcgaa aacggcaacg caaaagccaa gaacacctgg gattcattca tcaagttatt
    25381 caaagcggta tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac
    25441 aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc caaatcggga tacgcatatg
    25501 gatgaagcct tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc
    25561 agaagcaaga agtgctacag caaataaagt ggataagata ccagattttg ctacagcaat
    25621 caagatacca ttgctcatag ctgatggatc agataaggct tcatacagac caaaaaagga
    25681 aggatcttgg acagaaccgg ttttgaagac agcgacaccc atgatcaata gcagtgcatt
    25741 gactagaaaa gccatcgaca attggatatt agagtcccaa gtagaaaaac gtacagcttg
    25801 tgctacatct tcttcgtctt ggtgatcaat cttccgagtt tgagaaactg ctgaatgaag
    25861 atataagtta tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt
    25921 ttcgccgccg atgaccggtg agtctgagaa tgtatgactg ttagggataa agccagcaaa
    25981 taaactgtgc caatctgggt tagacaagcc aacttgatag acaaaaacga ataaaatgac
    26041 gaaaattaag cagacaacca gtgcttcgat cttacgaaac ccgacttttg tcagtagtaa
    26101 gagaacgaaa acatctaata cagtaataaa gacggcaatc accaatggaa tatcaaaaag
    26161 taagtagagg gcgattgctg caccaatgac ttctgcgatg tctgttgcca taatggctaa
    26221 ttcagtcaag atccataaaa tgacacccaa tgtcttactt gtgcgtgctc ggattgcttg
    26281 ggccaaatcc atttgggtca cgattcctaa ttttgctgcc atatactgta aaagcatagc
    26341 aatcagacta gacatcaaaa taactgacat caataaatat tggaaatttt gtccaccagt
    26401 gatcgaagtg gaccagttac ctggatccat ataaccaact gctactaatg cacctggtcc
    26461 agagtaagca agaagtgttt taaaaaaact tttccccttt ggtacatcaa ctgttccatt
    26521 tacttcagct aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt
    26581 gttggattga ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca
    26641 cctcaaaaag aaaaaattaa agaaaaagat tcggtgtgcc taatttattt tcgtggcgcg
    26701 tcttgttaga tgacgaaaga aattttgttc tcctctaaaa aaaccactat tttttgcatg
    26761 aaactaaaga atacgccttt cttttttcaa taaactttag taaaataagt cattgagagc
    26821 aattcggaaa tagaaaggag agatttatat gttatcaaca ggtattgtcg tacttattgc
    26881 tattattgct ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa
    26941 agactattta gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc
    27001 aatggggcaa aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca
    27061 agggaaaaaa taggttctga cccaacgact ttttacgggc tgattttttc agcccgtttt
    27121 ttatttcgta atttgttaga ctattcagat tattcagcaa aaaagacaag cacagacaat
    27181 cctaggctct tgataaagac aaaggaggag taaaatgtcg atttttagaa agttaggctg
    27241 gttttttcgt caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc
    27301 cttggtacag ttagttcctc caaaagtgat tgggatcatc attgatgaga ttgctgataa
    27361 aaatttatca gtaaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttttt
    27421 acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga
    27481 gaaagaactt cggcagcagt tattcaaaca ttttacagaa atggacaatc tcttttacca
    27541 aaaatatcgg actggggatt tgatggctca tgcaacgaat gatttgaatg cgatccagaa
    27601 tgtagcagga gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat
    27661 cattgcgatg attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc
    27721 tttgctggcc gtcacttcgc gagtgctggg gtcaaaactc catgatgctt ttcgtgattc
    27781 gcaagaagct ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt
    27841 cattaaaaca tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga
    27901 tgcaattatt aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac
    27961 cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga
    28021 aggcacgatc tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg
    28081 gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga
    28141 tcgcgtcaat gaacttttgc atgaaaaaac gcatatcgtg gaacaacttg gtgcagtgca
    28201 gaccccggca aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga
    28261 atcaccagca ttagaacaaa tcaagtttgc aatccaagaa ggagaaacat taggtattgt
    28321 tgggaaaact ggtgcgggaa aaacgacgat catcaaatta ctgatgcgtg aatacgatga
    28381 ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt
    28441 attaggatca atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga
    28501 taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt
    28561 agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga
    28621 acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat
    28681 caaaaatcct gaattattaa tattagatga tgcactttct gcagtagatg ccaaaacaga
    28741 agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcatttcagc
    28801 tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat
    28861 cattgaacga ggaacccatc atgagctgct tgcactaggg ggctggtatg aacaaatgtg
    28921 ggagaaacag caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat
    28981 caatcggaat ggacaaaatc gattccactt aaagaacaga aaaagattat caaacgactg
    29041 atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgct
    29101 ctgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg
    29161 gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga
    29221 gtcatagtca aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg
    29281 aagacctatc agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt
    29341 tattttgatc agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg
    29401 ctatttgaat tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct
    29461 tcattcattg ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg
    29521 ccaattttag gaatcgtcat ctggtattac cagaaattta gttctcgtat ttaccggaat
    29581 atgcgagaaa aattaagtca gctgaatacg aaattgaatg aatcgatttc cggcatgcgt
    29641 attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat
    29701 gagtatttag acacgcgcta tgcgatgatc aaaacgaatt cgattctgtt aagtccaatc
    29761 atcaattttc tatatgcatt ggcgattgcg ttggtgttga ctttgtttgg gatcgatgcg
    29821 ttgcattctc ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt
    29881 tttaatccaa tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct
    29941 ggtagtcgta tcttgaagat tatggataca gaagaatatg caccacaaca aaaccctgga
    30001 gcagatcaaa tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat
    30061 ggagagcaca atgtattaaa caatatctct tttgttgcta atccgggaga gaccgttgca
    30121 ttagtgggac acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac
    30181 gaattttatg aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa
    30241 gagttacgta agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc
    30301 gcaggaaata ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct
    30361 gagtttgttc aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg
    30421 atcgaacatg gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca
    30481 atcgtgactg atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa
    30541 acagaaggat tgatccaaga aggattgaca aaaatgcgac aaggacggac aacgattgcg
    30601 attgcccatc ggctgtccac gattcgggat gcagaattga ttttagtttt agacaaaggt
    30661 catatagtag aatgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
    30721 atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg tgacagggct ccgttcagct
    30781 ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga tttttgcata aatttggctt
    30841 atcaccgata agctggtact ggaacaccgt atattagaag ttgggacagt agcatagtag
    30901 cattttgacc taagcactag agtgaaaata aaacagagct tcccagattc ttcatctgtg
    30961 agcgttagtg tcgtaattac cagatttaaa aaaaatcacg acagctagtc agaagtgccc
    31021 aatagtcgct aagaaagcgt tggcaaaagt gcttgctttc gataatataa agaaaacgat
    31081 acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggttccat
    31141 ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc atggagagga
    31201 gtaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag
    31261 aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc
    31321 gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcacaaca
    31381 gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat
    31441 tgaatataca aaagaggctc tacaagtcca gttgaatgat tcggtgtata ttgcattgac
    31501 ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt
    31561 gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa
    31621 gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt
    31681 gcatttagtc aatgcacaag gcattcaaag ccaacaagac aatacctacc tgtttactac
    31741 aacaatacag gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga
    31801 ttcagtccat ttttttcgat tcgtcacaca cctacgtttt tttctgacac gcgtcctaga
    31861 tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg taccaggtta ttcaaaaaaa
    31921 atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg agatattcaa
    31981 ctaccagata tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca
    32041 tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt aacaagaacg catgaaataa
    32101 gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg
    32161 aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc ggcgggaagg aaaatatcaa
    32221 tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa
    32281 tgatgagatc ttgaaaaata tggacggtgt tgtgacggta atgcgagcgg gaggacagta
    32341 ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa
    32401 ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga
    32461 tggaatatcg ggctgtttcc agccattcct tgctattcta gctgctggtg ggatgattcg
    32521 tggactgaca gcattacttg tcttcttagg cgcatttgac cgcggttctg gcacatttgt
    32581 tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac
    32641 agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttggcg cagcattaat
    32701 gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccta tttatcaaac
    32761 agtattcggc attccctgga ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt
    32821 tattgtgttg ttagctagcc aaattcagaa gccaattaaa aagttagtac ctgagatgat
    32881 cgcgaatttc ttcgttccat tcttcaccgt tcttattaca atgcccttag gttttttact
    32941 cgttggacca gcattcacat tcgcaactga tattttaatg gctggatttg aaacactttt
    33001 agcactttcg ccagttattt atggagcaat cgtaggcttc ttctggcaaa ttctggtgat
    33061 gtttggtctc cattgggcaa tcgtaccaat gggattgatg caattttctg tgaatggttg
    33121 gcaaaatatc atgacacctg ttgcagtagt gtcttttgga taaactgcag cacttactgc
    33181 actctacttt aagcttagaa acccaaaaga caaagcaatt gcaataccag ccattgtttc
    33241 tggtattgtg gggattacag agccagctat ttatgggttt acattgccac gtaaaaaaat
    33301 atttattttt acctgtgtcg gtggtgcgtt agggggcgcg tatagtggat taatgaattt
    33361 aacttcttgg aatcaaggtg gactaggcat ctttacaatt cccaactata ttagaccaga
    33421 tggtgattta acggatgtga ttaatgtatt gattgggatc gccattgcga tggtggtatc
    33481 ctttgcgctg acttttttct tttggaagga ggaagcaggt gaaacagatg atagacagaa
    33541 aagatcaggt aaagaaattg taaaaacacc gatacaagga caaatcgctc cactgaatgc
    33601 agcaaaggat gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga
    33661 aaaaggagaa gtacgtgccc cttttgacgg aacagtcatg accctgttcc cggcgaaaca
    33721 tgcaatcgga atggtttctg aagcaggtct ggaattgctg attcatgtcg gattggatac
    33781 tgttcagttg gaaggaaaat attttgaaag cttggtgcag caaggagaaa gagtgaaaca
    33841 aggcgatctg ttgctccgtt tcgacaagaa gaaaatcgaa gaggaaggat acagtttaga
    33901 aacacctgtg atcgtcacca attacacgga ttatttggat attttggaaa atcaagggga
    33961 gacggtcggg ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaaagaa
    34021 tggactaggc ttaactattc tttttattcc acttctttat tttcgaaatt gaaaggctct
    34081 ttcatgatgg ccgagctgat agggcgttta tcgaattttt gtcctacaga gattagatgg
    34141 actaaattct gttgataatt ttctgcaaaa acacaactat ataacacatc tagaagataa
    34201 caaatcgatg tattgatcgt aaaattacca attttcgaat acagtcgttc gcgtgtcgta
    34261 agtcgcaaag cggctgtact cattgaagat agcgttgaat caccgatact ggtcaaagct
    34321 aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca
    34381 cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc ttcatagaac
    34441 gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg
    34501 aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga
    34561 agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcttga
    34621 atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg
    34681 gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcatct
    34741 aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg
    34801 agtgtggacg ggtggacata acatcgctca gcaatctctt ggatggtcat ttcgccaagt
    34861 gctggagaat cgcttaagag aaactgaatc acttcatttt ctgaaggaga aaaggagatt
    34921 tgtttgattt tttcacgtaa taacatgtag ataccttctt cttgtttttt tacgtaaatt
    34981 ataacttttt tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa taaagacatt
    35041 ttttgacaag atatttacaa aaatccagaa gaaaacacta acttttgtaa atgttactgg
    35101 aaagaaacga atttctattg tttgttttgt ttacaaaaaa gataataaaa gtgtaaaaag
    35161 caaaggagtg agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt
    35221 tatacccccg agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa
    35281 atctggctcg tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag tcagatgtct
    35341 cagcgtatgt gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa
    35401 gaagcattga aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca
    35461 cgaatcaaag acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt
    35521 gctggcggga tattcaaagc ttttcgtacc gttccagtgg ttttggcaat cgttgaagac
    35581 atgaagcgtt tatgtccaaa tgcttggctg atcaatttcg cgaacccaag cgggatggtg
    35641 acagaagcag ttatcagata tggcaaatgg gataaagtga tcggattatg caatgtgcca
    35701 gtaatggcga tgatgacaga accaacaatg cttggcaaga aaaaagaaga gctgatctac
    35761 aagtttgccg gtctaaatca cttccattgg cacaaagtag cagataataa aggaaatgac
    35821 cgcacggcag aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc
    35881 tttgacatcc cgtttttcaa agaacagtta aaacaaatga acatgatccc ttgtggctac
    35941 caccgctatt actaccgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg
    36001 attggtacaa gagcgcagca agtgaaagaa acagaagcag agctgtttga attatacaaa
    36061 gatccagaat tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc ttattattca
    36121 gatgctgcct gtgaaacgat tgcttcgatc tatggaaata aagaaacaca gatcgtggta
    36181 tctacaaaga atgatggagc tgttcctgat ctgccggcag attgtgtagt agaagtcact
    36241 gcctatgttg gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc tgctgctgaa
    36301 agaggctggt tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agccgtgact
    36361 ggtgattatg gaacagcttt acaagcgttt acgatcaatc cgcttatacg atcaggaaag
    36421 acagcaaaac ggatcatgga tgagttgttt attgcacatc agcagtattt accgcaattc
    36481 aaggaaacaa tcagccggtt aaaagcagaa ggaatcgaag tccaagatga actggcaaga
    36541 gaattatcaa gcaatacaca tgtaatggag gaagtatgat gggattcaga aaagattttt
    36601 tatggggcgg agctacagca gccaatcaat gcgagggcgg ttatgatgaa ggcggacgtg
    36661 ggttagcgaa cgtagatgtt gtgccaatcg gagaagaccg ttttcctgta attactggaa
    36721 aaaagaaaat gtttgaattt gatgaagatc atttttatcc agcaaaaatt gcgatcgata
    36781 tgtatcatca ttataaggaa gatattgctt tgtttgcaga aatggggttc aagacttatc
    36841 ggatgtccat tgcttggagt cgtatttttc caatgggcga tgaaaaagag ccaaatgaat
    36901 ctggtttgaa attttatgag gatgtcttta aagaatgtca aaagtacggg atcgagccgc
    36961 ttgtgacgat tacacatttt gattgtccga tgcatttgat caaagaatat ggtgcgtggc
    37021 gttccagaga actcgttggt ttttatgaaa atctgtgccg agtcatcttc aatcgctata
    37081 aagggcttgt caaatattgg ttgacattca atgagatcaa tatgatcctt catgcaccat
    37141 tcatgggagc agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag
    37201 cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc
    37261 cggagaacaa agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc
    37321 ctgaagatgt ttgggcttct agaaaatcag atcgagaaaa ttatttcttc attgatgtcc
    37381 agtcacgagg agaatatccc gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga
    37441 tcgaaatgga acctggggat gccgcattgc tgaaagaaca tactgttgat tttatctcct
    37501 tttcttacta ttcttctcga gtttcgacta ctgacaacga actcttggaa cagactgcag
    37561 gaaatatctt tgcttctgtc aaaaatccat atctggaagc aagtgaatgg ggttggcaaa
    37621 ttgatccatt aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccac
    37681 tatttattgt agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat ggttatgtgg
    37741 aagatgacta tcgaatcgac tatctggcag cacatatcca ggcaatgaaa gatgcagtag
    37801 aattagatgg agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag
    37861 ctggtacagg tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gacaacgaag
    37921 gaaatggcac attagcccgt tcgaagaaga aatcattcaa ctggtataaa aaagtaattg
    37981 attcaaatgg agaaaatctt tcgaatgatt aatcaatgat ttataaaagt ctattgtttt
    38041 tcagcggatt tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt
    38101 tttttcgcat tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt
    38161 atgatgtaat cgttattggc gcaggtcctg ctgggatgac tgctgcttta tatgcttcac
    38221 gttcaaactt atccgtattg atgatcgaac aaggcgcccc tggcggccaa atgaataata
    38281 cggcagaaat cgaaaactat cctggtttcg attcaatcat gggtcctgat ctagcttata
    38341 agatgtatga aggtgtttca agatttggga cagaaaatgc ttatggaatc gtgcaagata
    38401 tcaaggatca cggcagccac aaagaagtta tttgtgaaga taaaacttac gaaggaaaaa
    38461 cagtcattat cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat
    38521 ttgctggacg tggcgtttct tactgtgcag tttgtgatgg tgcgtttttc cgtaacaaac
    38581 gtctagtagt gatcggagga ggagactcag cagtagaaga agccatctat ttgacacaat
    38641 ttgcttctga agtagtcatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc
    38701 aagaccgtgc atttgcgaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa
    38761 tcgttggaaa cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg
    38821 aatttgattt agcagcagac ggagtattca tctatgttgg actggatcca ttaactgcac
    38881 cttttagaga agcaggtttg acgaacgaag ctggctggat tccaacagac aacgaaatga
    38941 aaacgaatat gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa
    39001 tcactacagc tgttggtgac ggcggtattg ctggacaaca agtgtataaa tacttggagt
    39061 ctttagaaga aacaacagca taagtttaaa aagagtgaaa gaacggtttc aattgttctt
    39121 ttactctttt ttttcgcgtg aaatccactg aatccgattt atgtgaatac ttgcaatcat
    39181 gcttgataaa agaatccttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    39241 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    39301 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    39361 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    39421 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    39481 tctatacggt aagacaagca acagaaggac tagcacgttt catggataca caaggtgaag
    39541 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcagtca ccagaatttg
    39601 caatggaagc agccaaaaca ctggcacatc acaatatccc gtcttatgta ttcgaaagtc
    39661 tgcgtccaac accagagctt tcttttgcag ttcgtcattt gaagacgttt gcgggaatca
    39721 tggtaaccgc atctcacaac ccagcggctt acaatggata taaagtctac ggagaagatg
    39781 gcggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    39841 atccgctaaa agtcgaagta ttatctgacg aagaagctaa acatagcggg ttgatcacga
    39901 tcatcggaga agaagtggac gctgcttatc tagaagaaat caaagcagtg acgattgatc
    39961 gtgaattggt tgaaacaatg gggaaagatt tgaaacttgt gtacacgcca cttcatggaa
    40021 cagggaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    40081 ttcccgaaca agctgtggca gatccagatt tcactacggt caaatcgcca aatccagaag
    40141 aacattcagc ttttgaatat gctatacgac ttggagaaaa agaaggagct gacttgctga
    40201 ttgcaacaga tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca gatggttctt
    40261 atgaagtatt gactgggaac caaatcggag cattgtttgc tcaatatatt ttggaagcac
    40321 atcaatcaag aggcacttta ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg
    40381 aattgcctac agcaattgcg gaacactacg gtgctaccat gttcaatgtg ttgacagggt
    40441 ttaaattcat tgctgaaaaa atccaacaat tcgaagaaaa ccattcacat acttttatgt
    40501 ttggattcga agaaagttat ggttatttag ttaaaccttt tgttcgcgat aaagacgcga
    40561 tccaagcatt gcttttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt
    40621 tgtatgacgg cttgcaaacg atttttgaaa cctatggcta tttcgcagaa aaaacgattt
    40681 ctgtgacaat gagcggacaa gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc
    40741 gtaaagaagc accgacagaa tttgccggag caaaagttat tcaaacagaa gactttaaag
    40801 aattgacgag aacagaccgt gaaggaaacg tagaaaaact cacaacaccg ccatccgacg
    40861 tattgaaata tacattggaa gaaaatggct ggattgctat ccgcccttca ggaactgagc
    40921 cgaaaatcaa gttttatatt ggtgtaaaag gaagttctac agaagacgca gccgaaaaag
    40981 tagcctctta tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact tgagactgat
    41041 ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc
    41101 agacacgcaa aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc
    41161 cactcgtctt aaaattctgc tatatctgaa agacggggaa caaaatgtga cagcaatcac
    41221 ccaagcagtg gaaatggagc agtctgctgt atcgcatcaa ctacgtttat taagagaaaa
    41281 tcatgtcgtc aagtctcatc gcgagggaaa agcgattttg tatagtcttg atgattctca
    41341 tgtactggat attttgaacc agacactcaa acatgtggaa catcaataac gataggtgca
    41401 aatagataaa cactggagtg tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt
    41461 taagaaaaag acaaaaatga tcaatcgctt ttttctgttg tttcagttcc ataataacta
    41521 tgatcttcta ggtctaaagc attcaaaata agcgaagccg tttcttcaat agataaagat
    41581 gctacattga tcactacaca tcctagtttt tcataaagat cattcgcaaa atcgagttca
    41641 gctctaattt tttcgatatc ggaataagca gtatcagggt ttaatccgta agagcgcatc
    41701 cgttctttac ggatattatt taaaatatct gggttatttg ttagtcccac gattttttta
    41761 ggatcgatct cccaaagctg tttgggaata tgtgcttgtg ggatcaaagg taaattggca
    41821 acttttaaat ttttgttagc taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg
    41881 cctagcaaaa cgacatctgc ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt
    41941 acagcaaatt ccattgcttt gatccgtttg aaatagttct cattcaataa atggagggca
    42001 ccaggttgcc gaattggctt gataccagtt aaacgttcaa tttctgatat cggtggcgtc
    42061 atcacatcaa aactaaataa ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt
    42121 tcttgattga ttaatgtatg caggacaagt gctttttctt ctaatgcatc ttgcaatgcc
    42181 tgccttaatg ttttttcatc tgtcacgaac gttctgcgga ataggttgaa ctcaacggta
    42241 ggatactgag ccatagtagc ttgcgctagt tttgtcgcag tttctccagc agagtcggag
    42301 atgacaaaaa aagtaacgat ttctgtagaa tcggaagtca tggataaaac ctcacttgca
    42361 ttttttttat gtttattata ccatactcta gaatgcgtac ttcgtaatca ttttgatttg
    42421 gagggataag tcatgaaaaa acagacattg atcgaacaat cattagaaac attgaaaaat
    42481 aacggtttta aatatactaa aaaaagagaa gcattgttaa cttatttagt gaagcgtaac
    42541 cgctacgtat ctgctaaaga agtttttgac tatatgaatc aggaattcaa aggggtaagt
    42601 tatgatacga tttaccggaa tctccatgat tttgcagaga tcgatttact ggaagaaacc
    42661 gatttaaatg gagaaatgaa gtttcgtttt cattgctgtc aggggaaaat cggccatcac
    42721 catcatttta tctgtacagt gtgcggaaca acgaaagaaa tccatatgtg tccgatgaat
    42781 tatttcgaag aacagctagg tggatgcacg atcgaagggc atagattcga gattttcgga
    42841 agatgtgaaa attgtcaata aggatttaaa taaaataaca tttgataatt ttaaaaaaaa
    42901 gcaagatttc cttgacggtc aaaacagcat tagatataat atttacagga cagtcttgtt
    42961 ttttgagata tatttcaata acaattacgg ttacttttta agctcggagg gagggaatta
    43021 acatgtcaaa aacagtcgtt cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca
    43081 aacgttccgt gtcaaaagcg ggtactttac aagaatctcg taaacgtgaa ttctacgaaa
    43141 aaccaagtgt aagacgcaag aaaaaatctg aagcagctag aaaacgcaaa aaattctagt
    43201 tctgtgatga attggagttg gtagtgtgtc actactaagt agattaaacg atgacatgaa
    43261 agcagcgatg aaagcgaaag ataaggaatc tttacaagtc atccgaatga tcaagtcatc
    43321 tatacaaaac gagcagatta aagaaggtca cgacttgaca gaagaagaag aactaacggt
    43381 gttgtctcgt gaaatgaaac agcgacgtga ttctttgcat gagtttgaag aagcaggacg
    43441 tgacgattta gctgaaaaag taaaaagtga aattgtaata gtcgaaaaat atatgccgga
    43501 acaactttcc gacgaagaaa ttcgacaact tgttcaagaa gcaatcactc aaactggtgc
    43561 ttcatcaatg aaagaatttg gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa
    43621 agcagatgga aaccaagtga atgcgattgt gaaagaacta ttacaatctt aattttatca
    43681 tttaaactgc ggacagtgat tatccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa
    43741 acagctagaa gaaagaatct tattctttct tctagctgtt tttttcctgt ttaatatctc
    43801 ttatgttaaa ttgtgtttga tttggttatt cttctggtaa ctcacttgct tcttgtttat
    43861 atcctgatac gcgatataca taataaaaga gcaagctgcc agcgtaacca agcaatgttg
    43921 ggacgaccca gccaagacct aatgcagcaa aaggaacata atgatgataa aatcctagta
    43981 agcttgctac gacagtttgc tgactcacta atgcaggcga atttgccaac atatccaata
    44041 cggctggaac ggcagaaaag aacatcgtta cttgatagac aaatggactc ttttggaaga
    44101 attttgctgt taaggaaagt aagattaacg caagtgacaa tggatacaaa agcatcaata
    44161 ctggtaaaga ccattggatg atattatcca atccggcatt cgcaacgaca aaagagacaa
    44221 aagtcgttaa ccgcagccat gtcatataac tgactttagg gaacagttta tggaaatctt
    44281 gtgcaaaaga aacaacgagt cccatagcag tagtgaagac acctaatgtg accattaccc
    44341 ctaagaagct agcaccgaag ttacctaaat aaaaattagt gatctgagaa agtgcagttc
    44401 cgccattttc tgataaagac atcttattca aactcatcgc accgagaagg accagaccaa
    44461 aataaatcat gacttctaac gcaacgctca ggctaccagc ttttgctgtt aattctgtga
    44521 cttttttatc tttaaaacca agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa
    44581 gtaaagctac tgcatcaacc gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg
    44641 tctgataaga atctgcagca gattggttta gattccccat gggttggaat aaagaaacga
    44701 caaaaatgat agctaataat gcaagaaaca aggagtttaa atactttccg acatatttga
    44761 ccaaacgtgt tggatgtaca gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata
    44821 aaaacatacc taatgaatca tatttttcag gcaaaaaggg tttagcagcc atttcaaaag
    44881 cagttgctgc tgtccgcggt gtaccaaaga atggtccgat cgttaaatga acaaggatca
    44941 agaacaaggc agcataccat tttccgacag gtttagctaa gtcatacaga ccatcacttt
    45001 ttgtcactac tacagctaag atagctaata atgggaaaag agcgcctgag atggcaaatc
    45061 ctacaccagc gctgatccag tttgatccag ccatttgacc taagtgtaca ggaaagatca
    45121 gattacctga cccgaagaac atcccgaaga taagtgaact gaccatcaat aattgcttcc
    45181 atgaataagt ttttgtttct tccataaatt tttcctccaa taattatcat tttgagtaac
    45241 aaaataaacg ttgcagaaac tagaaatacc actaatatca gattccatga aaaccaccac
    45301 cttaaaatat aaaaaaagcc cttttccact tgggaaaagg gctctagatt ctagaacggt
    45361 accacctttt ttcgcttaaa aaagcttctt cattcacgga cgcttttcga taaagttatc
    45421 cgctagcctg ataatggagg cagccacaat gatctacgtt ttcgacattg actcggaaat
    45481 gatgttcgtc tattcgattt attgtcattt ctcacttaca tgactcactg ggaataaatc
    45541 actgaatagc ctactgtttt cctcgacgct ttgctgtata tttatttgtt gacgttataa
    45601 tacacctgtt ttttttgaaa tgcaagaata tttcaaatat tttgttttct ttttttgaaa
    45661 caagaaaaat acccagagaa tacttgctaa gttagacgga atacttgtcc tctatcttat
    45721 gcatagctta catgatcagc tttttttatt caaacgagac ctttagagaa tggaagcggc
    45781 tagtttttta acgtttcttg gtacttatgg ttaagcaact cttttagaac cttttcttat
    45841 ttgctatata gtttcttctg agtatggtaa aataggaaag aattaatcga aaaaaagggg
    45901 atgtctattt ttgacggaag agtctagttc tttagagatt agattgacgg ctgctgatga
    45961 tgtcagcatg cttttaggtg cacacgacaa gcatataaaa ctgattgagg aaacaacgga
    46021 aacaaccatc catacacgtg gagaaatcat tcagatcatt ggtgagcaat cccagatttc
    46081 cttggctgcc tcagtcattc ggacattaca ggaattgatc aaacgaggaa tccacgtaag
    46141 tacgcccgat gttgtgacgg cattgaaaat ggggcaaaaa ggtacattag attattttgt
    46201 tgagatgtat gaggaagaga tcgtcaaaga ccgtaatgga aaacctatac gggtaaaaaa
    46261 cagcggccaa aagaaatatg tcgaatcggt agccaaacat gatatcgtat ttggcgtagg
    46321 accagccggg acaggaaaaa cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa
    46381 aggtgaagtg caaaaaatca ttttgacgcg gcctgctgtg gaagcaggtg aaaatctagg
    46441 ctttcttccc ggggatctaa aagaaaaagt ggatccttat ctgcggcctg tttatgatgc
    46501 tttataccaa atttttggat tggatcatac gaaccgattg atggaacgag gcgtaatcga
    46561 aatcgcacct ttagcttaca tgcgggggcg aacattggat gatgcattcg ttatcttgga
    46621 cgaagcacaa aacacgacag tcgctcagat gaaaatgttc ttgacccgtc tcggttatca
    46681 atcaaaaatg attgtcaatg gggatacaag tcagatagac ttgcctcgag ggacgacaag
    46741 tggtcttgtt catgcagaac gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt
    46801 tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa
    46861 ggcagatctc catcaagaat aagctttgct taggaggata agcacatgtt gaataaaaca
    46921 ctgcagaaac tacaagaaaa acttggaaag gcagcattgc ctttcctgct gttcttgttt
    46981 tttatcatta tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa tgatttcaga
    47041 gaaggccaag tagcagaaga aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa
    47101 accgaacaaa aaagaaaact ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa
    47161 gatcttgcag atgaccagaa caatcggatc aagcaactat ttgaattgat cgataagacg
    47221 aatgatgcca tcaacaaatc ttatgacgaa aagattgata aggcaaagga agatgaaaat
    47281 atcccaaagc ctactacaga agagcggatc gccgcgctga agaaaaattt tgaaaatgtg
    47341 aatgcagaaa atgttgtgtt ttatcaaaaa ctaccgaaca atttttattc gacgatattt
    47401 gaaatgactg aaactgagat tcatactgtg agggatgaga gtctgaagct cattgacgaa
    47461 cagatgagca aacaagttcg tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag
    47521 caaatccaat atttgaatgt cacaccagca caacaacaaa tgattcgcta cttagtggat
    47581 caaggtatcg tagtgaatga cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc
    47641 gcacgagaag ctgtacaacc agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt
    47701 aatcaaatcg atgcttcagc tatgaaaaaa ttggaattac taggactgac taatcaaaca
    47761 acttcgattt ttccgctaat tgccatggtt ttagctgttt tgcttcaaat cggcgtactg
    47821 atttataatt cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt gcttttctat
    47881 gtgacagcta tgtcagtcag catagtgctg atgaaatttt tccaattatt ccaaacggaa
    47941 caagctactt atatcccgct gttttatccc gctgcttttg caccacttgt tttaagcttt
    48001 ttcttgaatc gaagagcagg gatcatggct ggtattttcc aagcagtatc cgcacttttc
    48061 attttttatg attcgattgg cacgaacttt ctaacgatta tcttgatgtc ttatgttttc
    48121 tctgggctca tggcaacgat tgtcagacgc aaacggatct cagaacaagg attatctgct
    48181 gccatgtggg tcattatttt tcctgtgatg atggatgtca ttttgatcgt ctatcaagga
    48241 atgagctttg gtgatgcaac gacttggtta atgctcgtct gcgggttcac tggtgcactc
    48301 ttctcattct tgctgacgat cggtctacac ccatatattg aattgatggt caatgatgac
    48361 agtgtaattg tattaaatga actcagcaat ccaaatcatc ctttattgaa acagttgctg
    48421 gaagaagcgc caggtactta tcatcatagt atgatggtag ctaatctaag tgctaacgct
    48481 gtagcagaaa tcggcggacg ttctttgcta actcgagtag cttgctatta tcatgatatc
    48541 ggcaagatca aacatgctag ctttttcgtt gaaaacttgc cttcaggagc ggaaaacccg
    48601 cacaatttct tattgccaga agacagtaaa catattatct ttggacatgt gacagatgga
    48661 gccaaaatat tggaagatta taatatgccg caaatggtga tcgatatttg caaacagcat
    48721 catggaacca cgctaatgcg ttatttctac atcaaagcga aagaacgcaa tccggaagta
    48781 acagaagagc aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc
    48841 aatattgctg acagttgtga ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa
    48901 atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt ccgatggtca attagacgat
    48961 agtggactga ccttgaaaga aatccgaaaa gtggaaaaat cattaatcag cggtttaagt
    49021 agcaccttcc attctcggat caaatatcct aaaatgaaaa cagaagttga gaagataaag
    49081 gaagtacaag aagaatagaa agaagcgatg tataatggat attaccttta tggatgaaac
    49141 aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc
    49201 ttatttaaag ctgccagagg aaacagaaat gtctgttact ttcatggaca atgcggcaat
    49261 tcaagtaatc aatcgtgatt accgtggaaa agatgcaccg acagacgtca tcagttttgc
    49321 tctggaagaa gaaggcgaag acgaaatccc agtgattttt gaagatggag agaatccatt
    49381 acctcgtgta ctaggagaca tcatgatctc tattgaacgt gctgctgaac aagcagaagc
    49441 ttacggccat tcatatgatc gggaacttgg ctttttagcg gtacatggat ttttgcatat
    49501 caacgggtat gaccatatga caccagaaga tgagaaagaa atgtttggtt tacagaaaga
    49561 gatcttggat gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa catttcctga
    49621 tgtctgtgga atttgctgtc caaggagtca aaaccgtttt tgaggaagaa cgcaatatga
    49681 ggaaacatgt tgcgttcggt atccttgctt tatttatggg cgtgattttc cagcttgatc
    49741 ggatagagtg gttatggctg cttttagctg tttttctcgt ttggatagta gagatcatca
    49801 atactgtttt tgaaaatgta gtggacatgt ttacggattt tcattttcat ccgattggga
    49861 aaaaaatcaa agatatggca gctggagcag ttttagtgac cgcttgtttt gctgtcatca
    49921 ttgggttgat tttatttgtc cctaaaatat atcatttgtt gtttaattaa aaaggagaac
    49981 gtatatgcca gaacataaat cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa
    50041 atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg acaaggcaca
    50101 gacaactaga aataaaatcc aaggagtata tactacacca gaagctcagc tgatttttat
    50161 cgatacacca ggaatccata aaccaaagca tcgtctagga gattttatgg tagaagcagc
    50221 ttacagtgcg ttgaaagaag tggatgcagt cttatttatg atcagtgctg atcaaaaacg
    50281 tggccgggga gacgatttta ttattgaacg tctaaaaaac gtgcattctc ctgtttatct
    50341 gattatcaat aaaatcgata agatccatcc agacgagttg ctgggaatca tcgaagatta
    50401 ttcgacgcag atgccttttg cacaagtgat tccgatctct gctacagaag gaaacaatgt
    50461 cgaacgctta atggatgtat tagtcagtga aatgccagag ggcccacaat attttccaga
    50521 tgatcaagtg actgatcatc ctgaatactt catcgtttct gaattgatcc gtgagaaagt
    50581 cttgttttta acacgagacg aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa
    50641 gcgaaacgag aatgataaaa tccatatcca ggccacgatc atcgtcgaaa gagatagcca
    50701 aaaaggaatc attatcggca aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag
    50761 aaaggatatc gaaaatcttt taggagacaa agtatttcta gaactttggg tcaaagtcca
    50821 aaaagactgg cgagacaaaa aagtctatct gcaagacttc ggctaccgaa aagacgagta
    50881 ctgatattct ggacttctta taggttaggt atagaaataa actggataaa aatccgtacg
    50941 ttgagaaaaa cagcggaaga tcgaaacata aataatctgg atcggcagaa tctgcttttc
    51001 cagatttatt ttttaggagg gagaatatga gccagcttgt agaatcgaaa ggcttgattt
    51061 tatctagccg taattacaaa gaaaaagata aattagtcaa aatctttacg gaatcttctg
    51121 gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa tcagccatta gctccggctt
    51181 tgctcccctt taccgaagca gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt
    51241 taagcagtgt caaagacgtc catgcgtttc ggaacatcca agaagatatc tttttgagcg
    51301 gttatggtac gtatttgcta aatctagtag atgcagcaat cgaagatcat caatatgacc
    51361 cgcatttatt ccaatttacg caacaagctc tgcagcggat ggaccaagga gatgatgcag
    51421 aaatcatcac gaatatcttt gaagtgcaaa tactgcaacg cttcggaatc gcgccaattt
    51481 ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct tccaaatatg
    51541 gcggtgtgat atgcgagaaa cactggccaa tggacgaaca ccgttatcat gcagatcctc
    51601 gcgctgttta tttcgttcga atgttttcgg ctatttctta tgataagatc agtggaatcc
    51661 acttgaaaga agaaacgaag caggcaatcc gtgacctcat tgaccagttg tatgatgaat
    51721 atgtcggtat tcatctaaaa agcaaaaagt tcattgatca aatgaagtct tgggcacacg
    51781 tattaaagac gccagagaaa aatatgcctg aaaaagagat acctgaaaaa gaagaataaa
    51841 tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata cagggacttc cgtctattat
    51901 taaccgtaga ttaaatagat cgttcactga gaggatctat tatacaaacc agaaaactgc
    51961 aaaatgaata cgtacattga ccaagatcag taaaaaacac gtggtttagc gagttcggga
    52021 gagtggaagc cggacccaaa cgatttttga agaggcactt gcgagtacga taaatgaagc
    52081 tgccgaatct ttattcggaa gtagggtgga accgcgaaat tattcgtccc tatgtctgta
    52141 atcagacata gggacttttt gtcgttttct ttcctgtatt cttattgaca gcaacgctta
    52201 aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc gtccaagata tgattttaac
    52261 attacaaaaa ttttggtcat caaacggctg catgctgatg caagcttatg atacagaaaa
    52321 aggagcaggt acgatgagcc catatacatt cttacgggca atcggaccgg aaccatggaa
    52381 tgctgcttat gtagagccat cccgccgacc agctgacggg cgttatggag aaaacccaaa
    52441 ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca
    52501 agagctttat ttagaaagct tgcgtctttt aggaattgat ccattagaac acgatattcg
    52561 ctttgttgaa gacaactggg agaacccatc gatgggttgt gccggtgtgg gctgggaagt
    52621 atggcttgat gggatggaga tcacacaatt cacgtacttc cagcaagtcg gaggactacc
    52681 ttgtaaacca gttacttctg agatcactta tggactagaa cgtcttgctt cttatatcca
    52741 agaagttgaa agtgtttatg atctggaatg ggctgatggc gtgaaatatg gtgagatctt
    52801 caaacaacca gaatatgaac attctaaata ttcttttgaa gtaagcgacc aagacctgct
    52861 actaagcaat tttgatcgtt ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt
    52921 tcatccagcg tatgattata ttttgaaatg tagccataca ttcaatttat tagatgctcg
    52981 cggtgcagta tccgtaacag agcgtgcagg ttatctttcc cgtatccgca acatggctcg
    53041 tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta ggattccctt tattgaataa
    53101 aaacacagca aatccagaaa acgtgaagga ggctgaataa gatggcaaaa gacttattac
    53161 ttgaaatcgg gctggaagaa atgcctgctc atgtagtcac accaagcatg aaacaattag
    53221 aagaaaaaac agcaaaattt ttagatgaaa accaattgac ttatgatacg atcgaaacgt
    53281 tttctacacc gcgccgctta gcaatcaaag taacaaacat tccagaacgt caagcagaca
    53341 gcgaagaaga agtcaaagga ccagccaaaa aaattgcttt ggacgcagat ggaaactgga
    53401 cgaaagcagc tgaaggattt gtccgcggac aaggtctgac gacagatgct atcactttta
    53461 gagaattaaa aggcgtggaa tatgtctatg taacgaaata cactcacggc aaagaagcaa
    53521 aagaagtatt gacaggttta gcagatgtca ttaaaagcct cactttccca gtaacgatgc
    53581 actgggcaga atacgatttt gaatatattc gccctattca ctggatcgtg gcattgttag
    53641 atgacgaaat aatcccgttt gagatcttgg atgtcaaaac agacagagtt tcaagaggtc
    53701 atcgtttctt aggcgaagat gtgacattta cgcatgcaaa agaatacgaa gaaaaattga
    53761 atgaacaatt tgttattgct tcaccgcaaa aaagaaaaga aatgatcatc acacaagcaa
    53821 acgaattagc agaaaaaaat cactggatca ttgatctaga tgaagattta ctagaagaag
    53881 tcaacaatct agtcgaatat ccaactgtct tttctggcga cttcgaagaa aaatatctat
    53941 ctgttcctga agaagtacta gtcacttcta tgaaagaaca tcaacgttat ttcgaagtac
    54001 ggaacgatca aggtatgttg cttcctcatt ttatttctgc tcgtaatggc gatcgggtaa
    54061 aattggaaaa tgtggtacga ggcaatcaaa aagtattgac tgctcgttta gaagatgcgg
    54121 aattctttta taatgaagac aagaaagctt caatcgacca atacgttgaa aaattaaaaa
    54181 ctgttacttt ccatgaaaaa atcggtagca tctatgagaa aatgcaacgg gtacaagtca
    54241 tcgctcgcat cataggcgaa caagtaggac tatctgagga agaattgatg gatctgcaac
    54301 gcgctgctga aatctataaa tttgatctag taaccaacat ggtaggagaa ttccctgaac
    54361 tacaagggat catgggagaa aaatatgctc tgcttaaagg cgagaaacca gccgtagcaa
    54421 cagcgatccg tgaacattac atgcctatat ccagtgaagg agatttacct gagactacag
    54481 taggttctgt tttagcgatt gctgataaat tagatagtat cttttctttc tttgcagttg
    54541 ggatgatccc aagtggctca aacgatccat atgctttgcg ccgtcaagca tatggtgtcg
    54601 ttcgaatcat cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta
    54661 gtgaagttat cagtaaagat acggatcgtt ttggcattgg cttgtcagcg ggacaacaac
    54721 aggtgatcga ctttatcaaa ggccgtctgc gccaattatt gacaacgaag aatatccgtc
    54781 atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag
    54841 cagcacaatt gttcaaacaa catcttgcag atgaagactt caaaccttca atggaagcat
    54901 tgacacgtgt cgtgaactta gcgaaaaaag cgcagcttga aaaacaatca gaagtagacc
    54961 ctgagctgtt cgaaaacgac gcagagaaag aattacacaa agcagtagaa gcaatgaaag
    55021 caagttttgc tgaccaaacg atccacgaaa actacgacca gcttgtcgct cttcgtccac
    55081 tgatcgaaaa ttattttgat caaacgatgg tcatggctga tgaaccagca gtacgtaaga
    55141 accgactaac acaacttagt caaataactg ccatggcttt gtcattagca agcttggatc
    55201 aaatcatcac aaaatagtct tttgtttgaa aaacagagag catgcacgaa acgtgctgtg
    55261 tctctgtttt tttgttataa atataaaagc tgttattaat tctattttaa taa
//
LOCUS       DANKHK010000006        52414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000006 DANKHK010000000
VERSION     DANKHK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(124..501)
                     /locus_tag="PW109_000309"
     CDS             complement(124..501)
                     /locus_tag="PW109_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510191.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            757..1485
                     /locus_tag="PW109_000310"
     CDS             757..1485
                     /locus_tag="PW109_000310"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2510192.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1485..1739
                     /gene="purS"
                     /locus_tag="PW109_000311"
     CDS             1485..1739
                     /gene="purS"
                     /locus_tag="PW109_000311"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2510193.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1741..2412
                     /gene="purQ"
                     /locus_tag="PW109_000312"
     CDS             1741..2412
                     /gene="purQ"
                     /locus_tag="PW109_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2510194.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2413..4635
                     /gene="purL"
                     /locus_tag="PW109_000313"
     CDS             2413..4635
                     /gene="purL"
                     /locus_tag="PW109_000313"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2510195.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4620..6059
                     /locus_tag="PW109_000314"
     CDS             4620..6059
                     /locus_tag="PW109_000314"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2510196.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6082..7134
                     /locus_tag="PW109_000315"
     CDS             6082..7134
                     /locus_tag="PW109_000315"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2510197.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7131..7709
                     /gene="purN"
                     /locus_tag="PW109_000316"
     CDS             7131..7709
                     /gene="purN"
                     /locus_tag="PW109_000316"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2510198.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7706..9241
                     /gene="purH"
                     /locus_tag="PW109_000317"
     CDS             7706..9241
                     /gene="purH"
                     /locus_tag="PW109_000317"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2510199.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9257..10531
                     /gene="purD"
                     /locus_tag="PW109_000318"
     CDS             9257..10531
                     /gene="purD"
                     /locus_tag="PW109_000318"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2510200.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10653..11567
                     /locus_tag="PW109_000319"
     CDS             10653..11567
                     /locus_tag="PW109_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2510201.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTSTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11700..13265)
                     /locus_tag="PW109_000320"
     CDS             complement(11700..13265)
                     /locus_tag="PW109_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2510202.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13258..14709)
                     /locus_tag="PW109_000321"
     CDS             complement(13258..14709)
                     /locus_tag="PW109_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2510203.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14723..15739)
                     /locus_tag="PW109_000322"
     CDS             complement(14723..15739)
                     /locus_tag="PW109_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2510204.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15959..17047
                     /locus_tag="PW109_000323"
     CDS             15959..17047
                     /locus_tag="PW109_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2510205.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17101..18627
                     /locus_tag="PW109_000324"
     CDS             17101..18627
                     /locus_tag="PW109_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2510206.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18629..19834
                     /locus_tag="PW109_000325"
     CDS             18629..19834
                     /locus_tag="PW109_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510207.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20091..21068
                     /gene="guaC"
                     /locus_tag="PW109_000326"
     CDS             20091..21068
                     /gene="guaC"
                     /locus_tag="PW109_000326"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2510208.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21332..22756
                     /locus_tag="PW109_000327"
     CDS             21332..22756
                     /locus_tag="PW109_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2510209.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23217..24326
                     /gene="pdhA"
                     /locus_tag="PW109_000328"
     CDS             23217..24326
                     /gene="pdhA"
                     /locus_tag="PW109_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2510210.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24329..25306
                     /locus_tag="PW109_000329"
     CDS             24329..25306
                     /locus_tag="PW109_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2510211.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25331..26974
                     /locus_tag="PW109_000330"
     CDS             25331..26974
                     /locus_tag="PW109_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2510212.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26982..28388
                     /gene="lpdA"
                     /locus_tag="PW109_000331"
     CDS             26982..28388
                     /gene="lpdA"
                     /locus_tag="PW109_000331"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2510213.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28550..28825
                     /locus_tag="PW109_000332"
     CDS             28550..28825
                     /locus_tag="PW109_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2510214.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28831..29598
                     /locus_tag="PW109_000333"
     CDS             28831..29598
                     /locus_tag="PW109_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2510215.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29766..31595
                     /gene="typA"
                     /locus_tag="PW109_000334"
     CDS             29766..31595
                     /gene="typA"
                     /locus_tag="PW109_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2510216.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31856..33628
                     /gene="recQ"
                     /locus_tag="PW109_000335"
     CDS             31856..33628
                     /gene="recQ"
                     /locus_tag="PW109_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2510217.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33742..34044
                     /locus_tag="PW109_000336"
     CDS             33742..34044
                     /locus_tag="PW109_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2510218.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34047..35210
                     /locus_tag="PW109_000337"
     CDS             34047..35210
                     /locus_tag="PW109_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2510219.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35236..38664
                     /locus_tag="PW109_000338"
     CDS             35236..38664
                     /locus_tag="PW109_000338"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2510220.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38685..39809
                     /locus_tag="PW109_000339"
     CDS             38685..39809
                     /locus_tag="PW109_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510221.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39799..40236
                     /locus_tag="PW109_000340"
     CDS             39799..40236
                     /locus_tag="PW109_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2510222.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40220..40558
                     /locus_tag="PW109_000341"
     CDS             40220..40558
                     /locus_tag="PW109_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2510223.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40677..41237
                     /gene="rsmD"
                     /locus_tag="PW109_000342"
     CDS             40677..41237
                     /gene="rsmD"
                     /locus_tag="PW109_000342"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2510224.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41234..41725
                     /gene="coaD"
                     /locus_tag="PW109_000343"
     CDS             41234..41725
                     /gene="coaD"
                     /locus_tag="PW109_000343"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2510225.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41715..42758
                     /locus_tag="PW109_000344"
     CDS             41715..42758
                     /locus_tag="PW109_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2510226.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42917..43645
                     /locus_tag="PW109_000345"
     CDS             42917..43645
                     /locus_tag="PW109_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2510227.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43761..44006
                     /locus_tag="PW109_000346"
     CDS             43761..44006
                     /locus_tag="PW109_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2510228.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44003..44221
                     /locus_tag="PW109_000347"
     CDS             44003..44221
                     /locus_tag="PW109_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2510229.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44357..44896
                     /locus_tag="PW109_000348"
     CDS             44357..44896
                     /locus_tag="PW109_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2510230.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44889..47162
                     /locus_tag="PW109_000349"
     CDS             44889..47162
                     /locus_tag="PW109_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2510231.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47200..48246
                     /gene="holA"
                     /locus_tag="PW109_000350"
     CDS             47200..48246
                     /gene="holA"
                     /locus_tag="PW109_000350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2510232.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48335..48589)
                     /locus_tag="PW109_000351"
     CDS             complement(48335..48589)
                     /locus_tag="PW109_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2510233.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48989..49258)
                     /locus_tag="PW109_000352"
     CDS             complement(48989..49258)
                     /locus_tag="PW109_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510234.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49728..50183)
                     /locus_tag="PW109_000353"
     CDS             complement(49728..50183)
                     /locus_tag="PW109_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2510235.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50321..52249)
                     /locus_tag="PW109_000354"
     CDS             complement(50321..52249)
                     /locus_tag="PW109_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2510236.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17222 a         9007 c        11425 g        14760 t
ORIGIN      
        1 aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag
       61 gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag
      121 gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag
      181 caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata
      241 atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc
      301 tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat
      361 ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag
      421 caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc
      481 ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag
      541 ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt
      601 tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg
      661 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
      721 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
      781 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
      841 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
      901 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
      961 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
     1021 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
     1081 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
     1141 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc
     1201 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
     1261 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
     1321 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
     1381 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
     1441 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
     1501 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
     1561 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
     1621 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
     1681 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
     1741 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
     1801 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
     1861 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
     1921 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
     1981 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
     2041 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
     2101 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
     2161 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
     2221 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
     2281 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
     2341 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
     2401 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
     2461 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
     2521 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
     2581 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
     2641 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
     2701 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
     2761 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
     2821 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
     2881 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
     2941 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
     3001 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
     3061 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
     3121 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
     3181 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
     3241 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
     3301 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
     3361 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
     3421 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
     3481 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
     3541 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
     3601 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
     3661 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
     3721 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
     3781 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
     3841 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
     3901 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
     3961 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
     4021 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
     4081 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
     4141 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
     4201 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
     4261 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
     4321 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
     4381 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
     4441 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
     4501 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
     4561 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
     4621 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
     4681 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
     4741 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
     4801 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
     4861 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
     4921 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
     4981 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
     5041 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
     5101 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
     5161 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
     5221 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
     5281 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
     5341 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
     5401 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
     5461 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
     5521 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
     5581 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
     5641 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
     5701 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
     5761 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
     5821 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
     5881 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
     5941 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
     6001 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
     6061 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
     6121 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
     6181 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
     6241 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
     6301 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
     6361 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
     6421 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
     6481 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
     6541 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
     6601 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
     6661 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
     6721 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
     6781 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
     6841 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
     6901 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
     6961 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
     7021 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
     7081 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
     7141 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
     7201 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
     7261 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
     7321 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
     7381 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
     7441 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
     7501 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
     7561 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
     7621 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
     7681 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
     7741 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
     7801 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
     7861 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
     7921 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
     7981 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
     8041 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
     8101 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
     8161 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
     8221 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
     8281 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
     8341 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
     8401 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
     8461 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
     8521 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
     8581 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
     8641 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
     8701 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
     8761 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
     8821 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
     8881 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
     8941 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
     9001 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
     9061 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
     9121 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
     9181 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
     9241 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
     9301 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
     9361 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
     9421 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
     9481 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
     9541 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
     9601 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
     9661 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
     9721 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
     9781 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
     9841 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
     9901 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
     9961 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
    10021 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
    10081 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
    10141 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
    10201 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
    10261 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
    10321 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
    10381 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
    10441 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
    10501 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
    10561 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
    10621 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    10681 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    10741 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    10801 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    10861 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    10921 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    10981 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    11041 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    11101 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    11161 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    11221 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    11281 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    11341 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacatcaa
    11401 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    11461 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    11521 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    11581 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    11641 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    11701 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    11761 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    11821 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    11881 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag
    11941 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    12001 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    12061 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    12121 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    12181 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    12241 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    12301 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    12361 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    12421 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    12481 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    12541 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    12601 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    12661 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    12721 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    12781 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    12841 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    12901 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    12961 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    13021 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    13081 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    13141 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    13201 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    13261 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    13321 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    13381 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    13441 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    13501 aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg
    13561 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    13621 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    13681 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    13741 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    13801 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    13861 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    13921 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    13981 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    14041 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    14101 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    14161 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    14221 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    14281 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    14341 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    14401 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    14461 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    14521 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    14581 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    14641 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    14701 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    14761 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    14821 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    14881 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    14941 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    15001 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    15061 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    15121 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    15181 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    15241 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    15301 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    15361 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    15421 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    15481 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    15541 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    15601 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    15661 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    15721 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    15781 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    15841 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    15901 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    15961 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    16021 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    16081 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    16141 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    16201 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    16261 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    16321 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    16381 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    16441 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    16501 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    16561 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    16621 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    16681 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    16741 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    16801 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    16861 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    16921 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    16981 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    17041 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    17101 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    17161 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    17221 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    17281 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    17341 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    17401 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    17461 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    17521 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    17581 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    17641 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    17701 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    17761 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg
    17821 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    17881 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    17941 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    18001 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    18061 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    18121 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    18181 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    18241 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    18301 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    18361 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    18421 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    18481 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    18541 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    18601 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    18661 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    18721 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    18781 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    18841 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    18901 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    18961 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    19021 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    19081 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    19141 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    19201 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    19261 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    19321 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    19381 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    19441 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    19501 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    19561 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    19621 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    19681 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    19741 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    19801 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    19861 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    19921 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    19981 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    20041 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    20101 ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat
    20161 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    20221 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    20281 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    20341 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    20401 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    20461 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    20521 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    20581 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    20641 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    20701 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    20761 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    20821 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    20881 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    20941 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    21001 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    21061 caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa
    21121 taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc
    21181 agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac
    21241 agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa
    21301 agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg
    21361 accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa
    21421 tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc
    21481 caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg
    21541 cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg
    21601 gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga
    21661 agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga
    21721 gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa
    21781 agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag
    21841 taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc
    21901 aatctttttg attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc
    21961 ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc
    22021 gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt
    22081 gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa
    22141 acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga
    22201 tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga
    22261 tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat
    22321 cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat
    22381 ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat
    22441 tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc
    22501 ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg
    22561 gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg
    22621 tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta
    22681 cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa
    22741 gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta
    22801 aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa
    22861 accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg
    22921 tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa
    22981 aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
    23041 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
    23101 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
    23161 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
    23221 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
    23281 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
    23341 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
    23401 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
    23461 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
    23521 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
    23581 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
    23641 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
    23701 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
    23761 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
    23821 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
    23881 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
    23941 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
    24001 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
    24061 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
    24121 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
    24181 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
    24241 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
    24301 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
    24361 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
    24421 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
    24481 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
    24541 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
    24601 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
    24661 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
    24721 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
    24781 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
    24841 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
    24901 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
    24961 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
    25021 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
    25081 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
    25141 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
    25201 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
    25261 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
    25321 aaacaaagtc atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg
    25381 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
    25441 agaagttcaa aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa
    25501 aaatgtgatt gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga
    25561 tgcaccagga cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc
    25621 agctaaaccg gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg
    25681 agtcttccaa tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa
    25741 atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa
    25801 tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt
    25861 tccagaaggc actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca
    25921 caatagtgca ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac
    25981 atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc
    26041 atctgttcgt caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg
    26101 aaaaggtggc agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag
    26161 tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc
    26221 agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga
    26281 acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca
    26341 tacagctcca cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg
    26401 gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt
    26461 cgtgaaagct ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga
    26521 tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga
    26581 tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc
    26641 agacgaaatc aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat
    26701 gcgcaatgga acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc
    26761 agtcatcaac tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat
    26821 cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga
    26881 tcaccgtatc gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt
    26941 agctgatcca gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg
    27001 ctgtagaatt agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc
    27061 gcgctgctga aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt
    27121 gtttgaatgt cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag
    27181 agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa
    27241 aaacacaaga atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc
    27301 tattaaaaaa acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca
    27361 cactacgcgt cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg
    27421 caacaggttc ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt
    27481 ctactggcgg attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg
    27541 tgattggtgc cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg
    27601 aaggctcacc acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg
    27661 atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata
    27721 acggtgacag cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg
    27781 attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag
    27841 cgggtgtcga agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg
    27901 tacctaacat ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca
    27961 gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata
    28021 aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca
    28081 aagaagcaaa agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg
    28141 gtcgggcatt atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag
    28201 ataacgtgct tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat
    28261 tagctttagc aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc
    28321 atccatctct tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc
    28381 atatttgatt tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt
    28441 tagaattatc aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta
    28501 ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata
    28561 tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg
    28621 ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta
    28681 tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt
    28741 tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa
    28801 caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc
    28861 tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa
    28921 aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc
    28981 gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat
    29041 caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat
    29101 tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga
    29161 aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata
    29221 ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc
    29281 ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg
    29341 gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg
    29401 aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat
    29461 gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt
    29521 gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt
    29581 ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca
    29641 aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc
    29701 tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag
    29761 ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg
    29821 gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc
    29881 aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt
    29941 tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag
    30001 gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt
    30061 tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac
    30121 tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc
    30181 ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc
    30241 agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg
    30301 atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc
    30361 ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca
    30421 acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag
    30481 accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat
    30541 ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg
    30601 ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag
    30661 atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc ttagttaata
    30721 attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt
    30781 tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg
    30841 catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc
    30901 gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg
    30961 taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg
    31021 ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg
    31081 gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat
    31141 tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa
    31201 tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa
    31261 aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag
    31321 gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg
    31381 tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat
    31441 ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg
    31501 atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa
    31561 atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac
    31621 cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt
    31681 catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta
    31741 aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat
    31801 caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa
    31861 cgacttatta aaacaatact ttggctatga tgagtttaga cctggtcaaa aagaaattat
    31921 ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc
    31981 aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt
    32041 aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt
    32101 tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca
    32161 gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc
    32221 aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg
    32281 ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa
    32341 tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa
    32401 aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct
    32461 acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat
    32521 caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta
    32581 tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa
    32641 tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac
    32701 aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat
    32761 tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc
    32821 cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca
    32881 gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac
    32941 agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac
    33001 aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac
    33061 gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca
    33121 aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg
    33181 acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat
    33241 tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa
    33301 agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc
    33361 caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga
    33421 actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc
    33481 cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa
    33541 aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa
    33601 tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca
    33661 actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat
    33721 tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta
    33781 aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc
    33841 tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa
    33901 gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt
    33961 gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa
    34021 aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag
    34081 actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata
    34141 gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc
    34201 agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag
    34261 atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc
    34321 tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag
    34381 gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga
    34441 ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac
    34501 ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg
    34561 cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata
    34621 ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata
    34681 tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt
    34741 accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg
    34801 ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat
    34861 ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga
    34921 ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag
    34981 gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt
    35041 tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca
    35101 cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct
    35161 ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga
    35221 tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt
    35281 gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat
    35341 gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa
    35401 ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct
    35461 gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc
    35521 gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat
    35581 aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat
    35641 ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt
    35701 atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag
    35761 agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat
    35821 gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg
    35881 gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac
    35941 caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc
    36001 tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa
    36061 tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa
    36121 catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct
    36181 caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat
    36241 ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac
    36301 acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga
    36361 aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt
    36421 acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt
    36481 agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa
    36541 ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt
    36601 cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat
    36661 ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa
    36721 gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc
    36781 gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt
    36841 agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt
    36901 gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca
    36961 acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt
    37021 cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg
    37081 tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga
    37141 atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc
    37201 caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc
    37261 aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta
    37321 gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag
    37381 gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat
    37441 actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta
    37501 gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc
    37561 agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca
    37621 cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc
    37681 ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat
    37741 ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg
    37801 tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta
    37861 gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa
    37921 aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg
    37981 caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca
    38041 ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa
    38101 ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat
    38161 ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg
    38221 gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa
    38281 cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat
    38341 cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg
    38401 atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga
    38461 gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa
    38521 ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt
    38581 gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt
    38641 ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt
    38701 tgttttggct gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc
    38761 cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt
    38821 ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc
    38881 agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa
    38941 caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca
    39001 gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag
    39061 ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt
    39121 accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa
    39181 ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat
    39241 tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg
    39301 gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg
    39361 ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca
    39421 tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga
    39481 caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca
    39541 gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta
    39601 aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt
    39661 cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg
    39721 attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg
    39781 gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc
    39841 ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa
    39901 cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa
    39961 gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa
    40021 cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga
    40081 tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa
    40141 gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt
    40201 ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg
    40261 cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt
    40321 gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt
    40381 ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta
    40441 ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga
    40501 cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa
    40561 atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat
    40621 tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc
    40681 ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca
    40741 gaccaactac agataaagta aaagaatcca tattcaatat gattggacca tattttgatg
    40801 gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta
    40861 gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag
    40921 aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca
    40981 aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc
    41041 catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt
    41101 ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg
    41161 ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa
    41221 aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg
    41281 tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt
    41341 tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa
    41401 agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga
    41461 aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt
    41521 tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt
    41581 cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact
    41641 gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg
    41701 aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg
    41761 gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag
    41821 ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt
    41881 ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta
    41941 aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg
    42001 aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac
    42061 aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga
    42121 gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag
    42181 tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag
    42241 ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg
    42301 atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata
    42361 ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag
    42421 cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg
    42481 gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag
    42541 gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag
    42601 atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag
    42661 aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag
    42721 tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt
    42781 tgtgacacga gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa
    42841 aaacaaaaga aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact
    42901 gtttcttcaa ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa
    42961 aagaacaatg atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg
    43021 gatgtctatg atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct
    43081 attttataga gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag
    43141 tggagaagat ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat
    43201 gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga
    43261 acgggtctat gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg
    43321 aatcaacttt tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga
    43381 agagctgcca gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga
    43441 ttccgagcct cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc
    43501 tgggatcgga tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt
    43561 ccagaaagtg gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact
    43621 aaaaaacttc gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg
    43681 gacgctaatg gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt
    43741 atttttggat aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga
    43801 atgtctatct ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca
    43861 ataacagtca agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata
    43921 aagtatttat tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa
    43981 cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca
    44041 tgctttcttt atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa
    44101 gttgatcaaa gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt
    44161 atcgattttt atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta
    44221 atggatgacg gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt
    44281 cgttatattt aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa
    44341 cggaggaata taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa
    44401 agttactgat aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc
    44461 agtactgtta tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg
    44521 agaaaaaagg atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat
    44581 agatgaaggg tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa
    44641 tgcactccta cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac
    44701 acattttcca tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa
    44761 ttatctccat gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt
    44821 atctgtacac caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac
    44881 aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa
    44941 ttacgtcgat gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc
    45001 ttgccggaat ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta
    45061 ttttgtgtag cataacggta tccatttctt ataggctaca agagtattca tttttagagg
    45121 aaaaaacaac atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt
    45181 ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag
    45241 taaacacaga agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga
    45301 ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag
    45361 aagcttactt caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga
    45421 tcaccatgca aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct
    45481 ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct
    45541 ataaagatgc aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc
    45601 tgttcagtct atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc
    45661 gacgtatcgg atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt
    45721 atgtttctat tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc
    45781 gattgctagt caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct
    45841 tttggcttct tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt
    45901 ttttcatgag ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg
    45961 cctctcacgt cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt
    46021 ggcctatttt aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc
    46081 ttcctggtgt gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc
    46141 tgatagaaga gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc
    46201 tcattatagg acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt
    46261 gtatggatag gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt
    46321 ttattttttt gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg
    46381 gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata
    46441 ctggaggtca gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg
    46501 ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca
    46561 tcttgacaca cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta
    46621 ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt
    46681 tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca
    46741 gcggttatta tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag
    46801 attcgattgg tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga
    46861 atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca
    46921 aattaggtca tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag
    46981 caaaacatgg gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag
    47041 tattggagac actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga
    47101 tcacctatcg ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact
    47161 agtaattctc atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata
    47221 caagcagagt tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga
    47281 acagaaaagt ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag
    47341 ggcgaagatg acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta
    47401 gtagctgaag cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat
    47461 ccttattttt taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg
    47521 acggcttatt tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa
    47581 aagttggatg aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat
    47641 gtccaacagt tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc
    47701 tcaatcaaaa tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta
    47761 tccaaaatga tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc
    47821 acggtaagag aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg
    47881 acccaatata ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt
    47941 atgcaagggg aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt
    48001 ttacagacaa aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg
    48061 aaaatccatc catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat
    48121 ttgctggtac agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa
    48181 atcgaaaaag aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat
    48241 cgataagaag aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt
    48301 tgacaaataa aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac
    48361 gagatttgtc gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa
    48421 tagctttaac agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat
    48481 cttcaaattt tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag
    48541 cgttttcgct tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct
    48601 ccgataactt aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa
    48661 aggcagcgtg ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag
    48721 gatttttttt cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt
    48781 gggaggcaat agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa
    48841 tgggaatgtt tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt
    48901 gaaaagggat acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt
    48961 ataaccagta tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt
    49021 catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc
    49081 cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc
    49141 gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca
    49201 tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa
    49261 gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat
    49321 tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa
    49381 gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg
    49441 gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta
    49501 tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa
    49561 ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc
    49621 ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc
    49681 agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa
    49741 gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt
    49801 cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga
    49861 tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa
    49921 acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct
    49981 cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct
    50041 ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca
    50101 aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt
    50161 cctaaaaaaa gccaactttc catgaaatat attcctcact ttcttcgaaa caatcataac
    50221 atgtttttag aaagttacaa aagtaggaat tcatccttca agacctattc ataagctgtt
    50281 tttttcataa aataaacaaa atcataaaaa aacactttgc ttatttgaca gaagacagcc
    50341 gcgctttgta tagtggacaa gacttacatt tttttgaaga atcagaaaga tcaggtaaag
    50401 gtcttagtaa acgcaagcca ttcagaataa ccaatattgt gcttccttcg tgtcctacaa
    50461 ctccaagagg tagatttaaa aattgcagga agttagaagc aatcaacaag agaataacaa
    50521 gaatcgaaaa aataatattt tgtttgatga tccgccgcat ttttaaagat agccgatgac
    50581 tgtagacaaa tttatttaag tcgcttttca ttaagacgat gtctgcgata tccatagcgg
    50641 catctgtccc ttgccccata gcgacaccta aagaagcggc tgctaaagca ggcgcatcat
    50701 tgactccatc accaaccatc gcatttactt ggtaaagttc attctctttt ttgactagtt
    50761 gtgttttttc ttccggagta cagcttgcat agtaatgttc aatccctact tgttcagcaa
    50821 tggcctttgc tgtgccagaa tggtcgccgg ttatcatact tgtatggatg ttttgggatt
    50881 taaagtagga aattacttgt tgggtatttg cttttggaat atctagcaga cctaaaacag
    50941 caattaactg ctgatcttta gaaagataaa tcacggtttt gccttgattt tccaaacgtt
    51001 cgatcatttc cgcaatctct ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc
    51061 caactttcca ttgcgcacct aaataggtag tttgtaatcc aaaaccagta atttcttcta
    51121 ttggtaagta ttggatttct tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag
    51181 caagtggatg cgtcgttttt tgttccatag caacaagaag ttgttgtgcc aaatcctttt
    51241 catcaaagaa aaagctgtca gttacaactg gttttccttt agtgagcgtt cctgttttat
    51301 caaaggcaat cgcttttaat tcagaaagct tttctaaatg gataccgcct ttgattaaaa
    51361 tcccattttt tgcagaattt gaaagagcag ctagagaagc tggtgtagca gatgcgacaa
    51421 gcgcacaagg agaagccaca actaataata ccattcctcg ataaaagctt tcatcccaac
    51481 tccaacctag gaaaaagtgg gggagaagga tcattagagg aacagcaagc aaaatcaatt
    51541 ttacataatt attttcgatt cgttcgataa atgaagctgt ttgagttggc gtattctgcg
    51601 cttcttctac taattggata atcttagcaa agacagtttc ggaactcgtt tttgttactc
    51661 gaatcgtgat gggatttcct tgattcagcg ttcctgcata catctcatcg cctaaatatt
    51721 tttctacagg cacagattca ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt
    51781 ctaaaaagcc atctatcgga acagctgctc ctttagcgac aaataccaag ttatccacct
    51841 ttagttgaga aacatctact tcttgcattt gtccattttc ttttaataat tgggcttttt
    51901 gtggttgaag attcatcaaa gcagtaattt ctttttgact tttattagtt gtgtattcct
    51961 ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc tccttcaaag taattaccga
    52021 tcaaacaggc accaatagca gctaaagcca tcaataaatc cacattcagt gtccgatcat
    52081 tccacaattc tttcaagcct tccgatgttt gtttatatcc accgatgata atggcactga
    52141 tataaaaaag tggggtgatg gtaagagaga atcggtcgaa gataaaaccg aagatcataa
    52201 ataaaaggca gccgattgtt gctgcaaaag cttttttctg attagtcatc gttattagac
    52261 ctccttgtca aaacaaggat agcataataa aaatgtaatt gataataata acagctaaat
    52321 gagaacgata atggttatca tttggtgatt aagcttgatg ttgtttttat tatttataat
    52381 gattattatt aaagaaaata aaaaaccaga acat
//
LOCUS       DANKHK010000007        50465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000007 DANKHK010000000
VERSION     DANKHK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..446
                     /locus_tag="PW109_000355"
     CDS             <1..446
                     /locus_tag="PW109_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDL2510237.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            727..1947
                     /locus_tag="PW109_000356"
     CDS             727..1947
                     /locus_tag="PW109_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2510238.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1944..4664
                     /locus_tag="PW109_000357"
     CDS             1944..4664
                     /locus_tag="PW109_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2510239.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4648..5592
                     /locus_tag="PW109_000358"
     CDS             4648..5592
                     /locus_tag="PW109_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2510240.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5661..6671)
                     /locus_tag="PW109_000359"
     CDS             complement(5661..6671)
                     /locus_tag="PW109_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2510241.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6851..7195)
                     /locus_tag="PW109_000360"
     CDS             complement(6851..7195)
                     /locus_tag="PW109_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2510242.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7188..7622)
                     /locus_tag="PW109_000361"
     CDS             complement(7188..7622)
                     /locus_tag="PW109_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2510243.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7760..8497
                     /locus_tag="PW109_000362"
     CDS             7760..8497
                     /locus_tag="PW109_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510244.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8490..9701
                     /locus_tag="PW109_000363"
     CDS             8490..9701
                     /locus_tag="PW109_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510245.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9905..10687
                     /locus_tag="PW109_000364"
     CDS             9905..10687
                     /locus_tag="PW109_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2510246.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10757..11419
                     /gene="trmB"
                     /locus_tag="PW109_000365"
     CDS             10757..11419
                     /gene="trmB"
                     /locus_tag="PW109_000365"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2510247.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11517..13271)
                     /gene="ade"
                     /locus_tag="PW109_000366"
     CDS             complement(11517..13271)
                     /gene="ade"
                     /locus_tag="PW109_000366"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2510248.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLVSLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13403..14332
                     /gene="tadA"
                     /locus_tag="PW109_000367"
     CDS             13403..14332
                     /gene="tadA"
                     /locus_tag="PW109_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2510249.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14289..15338
                     /locus_tag="PW109_000368"
     CDS             14289..15338
                     /locus_tag="PW109_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2510250.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15335..15613
                     /locus_tag="PW109_000369"
     CDS             15335..15613
                     /locus_tag="PW109_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2510251.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15588..16058
                     /locus_tag="PW109_000370"
     CDS             15588..16058
                     /locus_tag="PW109_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2510252.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16075..16377
                     /locus_tag="PW109_000371"
     CDS             16075..16377
                     /locus_tag="PW109_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510253.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16394..16765
                     /locus_tag="PW109_000372"
     CDS             16394..16765
                     /locus_tag="PW109_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2510254.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16737..17096
                     /locus_tag="PW109_000373"
     CDS             16737..17096
                     /locus_tag="PW109_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510255.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17268..18275
                     /locus_tag="PW109_000374"
     CDS             17268..18275
                     /locus_tag="PW109_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2510256.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18300..19484
                     /locus_tag="PW109_000375"
     CDS             18300..19484
                     /locus_tag="PW109_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2510257.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19921..21504)
                     /locus_tag="PW109_000376"
     CDS             complement(19921..21504)
                     /locus_tag="PW109_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2510258.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYSELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22082..22225)
                     /locus_tag="PW109_000377"
     CDS             complement(22082..22225)
                     /locus_tag="PW109_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2510259.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22447..22926)
                     /locus_tag="PW109_000378"
     CDS             complement(22447..22926)
                     /locus_tag="PW109_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2510260.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22999..23283)
                     /locus_tag="PW109_000379"
     CDS             complement(22999..23283)
                     /locus_tag="PW109_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510261.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23361..23555)
                     /gene="ssrS"
                     /locus_tag="PW109_000380"
     ncRNA           complement(23361..23555)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PW109_000380"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23627..24913)
                     /locus_tag="PW109_000381"
     CDS             complement(23627..24913)
                     /locus_tag="PW109_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2510262.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25051..25722
                     /locus_tag="PW109_000382"
     CDS             25051..25722
                     /locus_tag="PW109_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2510263.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25688..26176
                     /locus_tag="PW109_000383"
     CDS             25688..26176
                     /locus_tag="PW109_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2510264.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26189..27136
                     /gene="prmA"
                     /locus_tag="PW109_000384"
     CDS             26189..27136
                     /gene="prmA"
                     /locus_tag="PW109_000384"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2510265.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGVKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27137..27901
                     /locus_tag="PW109_000385"
     CDS             27137..27901
                     /locus_tag="PW109_000385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2510266.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28134..30347
                     /locus_tag="PW109_000386"
     CDS             28134..30347
                     /locus_tag="PW109_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2510267.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30358..30804
                     /locus_tag="PW109_000387"
     CDS             30358..30804
                     /locus_tag="PW109_000387"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2510268.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30975..32234
                     /locus_tag="PW109_000388"
     CDS             30975..32234
                     /locus_tag="PW109_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2510269.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32323..33270)
                     /locus_tag="PW109_000389"
     CDS             complement(32323..33270)
                     /locus_tag="PW109_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2510270.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33664..34956
                     /locus_tag="PW109_000390"
     CDS             33664..34956
                     /locus_tag="PW109_000390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2510271.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34971..36740
                     /gene="aspS"
                     /locus_tag="PW109_000391"
     CDS             34971..36740
                     /gene="aspS"
                     /locus_tag="PW109_000391"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2510272.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36849..37724
                     /locus_tag="PW109_000392"
     CDS             36849..37724
                     /locus_tag="PW109_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2510273.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37990..39873
                     /locus_tag="PW109_000393"
     CDS             37990..39873
                     /locus_tag="PW109_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2510274.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39948..40232)
                     /locus_tag="PW109_000394"
     CDS             complement(39948..40232)
                     /locus_tag="PW109_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2510275.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40222..40956)
                     /locus_tag="PW109_000395"
     CDS             complement(40222..40956)
                     /locus_tag="PW109_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2510276.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41085..41708
                     /locus_tag="PW109_000396"
     CDS             41085..41708
                     /locus_tag="PW109_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2510277.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41900..43021
                     /locus_tag="PW109_000397"
     CDS             41900..43021
                     /locus_tag="PW109_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2510278.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43028..46156
                     /locus_tag="PW109_000398"
     CDS             43028..46156
                     /locus_tag="PW109_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2510279.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46175..49369
                     /locus_tag="PW109_000399"
     CDS             46175..49369
                     /locus_tag="PW109_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2510280.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49515..50222)
                     /locus_tag="PW109_000400"
     CDS             complement(49515..50222)
                     /locus_tag="PW109_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2510281.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16480 a         8860 c        10512 g        14613 t
ORIGIN      
        1 taaactatga gcttgagtat gaaaaatcta tttgttcaat cccttataat aatattgagg
       61 aaattataga aaaggatgct atcagcattt acaaattttt tatttttatt cctaatcgtc
      121 aattgaaagc taatatattt aaccatttga aaaagatgct aaatgtgtct gttactgaat
      181 cggcacctgt aaatattgaa atcgtacctc aaaatgtatc taaaggtgtg gtgtttgatc
      241 gtttggaatc aatctacaat ttaaagaaac ctttgagaat cgcgatcgga gatagtttaa
      301 atgacttatc tatgtttgaa agtgcagata ttagttttgc aatgggaaat agtcatcaag
      361 aaatcatgaa aaaagctgat tatgtcactg tttcaaatga taaagacgga gtatctcaag
      421 catttgaaag aattcttacc atataaacta attaaggtag tatagaacat agaattctaa
      481 atacggaaaa atggagcata aattccagta caaatagtac ttcttcagtg tcaaacgaag
      541 cgaatataac atagatattt aggattgtga caaaaatcat gtcacaatct ttttttcgaa
      601 taaacttatt ttttgaagtc ggccatttat cgtaatttct ttgcataata cgaacaaatt
      661 ttcggttatt gatgaaaaaa aacattgaaa atggtatgat aaaagaaaaa aaggaggaaa
      721 tttttaatgg tgaaatttat ccatgcggca gatttacata tggatcggtc atttgaagga
      781 ctgacaactc ttgataaaac tgtacaggaa aaactattga agacgaatct gaccgtttta
      841 tcaaatatca ttgaacaggc aataataaat aacgttgatt ttgttttact tgcaggagat
      901 aattttcatc aaaatcgtcc ttcattaaaa atacagaaac atttttcaga acagatgaaa
      961 cggttagaaa agaaccatat acctgtattt ataatattcg gaaatcatga tttctatcaa
     1021 aaagagcggt attggttctc ctttcctaaa aatgtccatt tatttacatc cgaaacggta
     1081 gagacaaaaa aaataacgat aaaaagtgga gaaactgtct ctctcagtgg atttagttat
     1141 cgtcaaccgt ggattcaaaa agacaaagtg atggaatttc cttctagaga actcacagat
     1201 taccatattg ggttgtacca cggggaacct ggcgtctctc aaaaaggaaa ctatgctcct
     1261 ttccaaccct caaagatgca agaaaaggga tacgattatt gggctttagg gcatatccat
     1321 gtgccaacgg tcttaaatga aaagcaaaca attgtttatc caggtgcgcc tcaaggtcat
     1381 actaaaaagg aacaagcttc tacatctatt ctactagttg aattaagtaa aggaagttgc
     1441 caaatacatc cgattaaagt agcagaggtc tattggaaga caaaggaaat atcattgagg
     1501 aaagcaagaa cgacaaaaga ggtcattgtt cacattcgtc agcagctttc aaaagtagag
     1561 gatggattta gtttaattga gataaaatta aaagattatg accatctcaa tacagatgtt
     1621 ttggaacgga tacaatcagg agagctatta gactatttgt tagaggaatt ctcagatagg
     1681 ataaatgatt tctttatttg gcgtatttct ctcatagaga atacttttta tacgaagacc
     1741 ccacttgctg cttctgctaa attgatggaa cagttatttc aatactatca gacacctcaa
     1801 gatttccaac aaattttaaa tgaagtatat agtcatcaag aagcggctag aatattgagc
     1861 gaactgccgg aatatcaaga agaaacactt gaaaaagcca agcagctgct taatcaagat
     1921 tttttatttg aggaggatca ggaatgaaga ttttaaggat cgagattgct gcttttggaa
     1981 aatggcgtca aaaaagtttt gacttttatt caggcaatca gctgatctat gggggaaacg
     2041 aagcaggaaa atcaacgatt tatcaattta ttcaagcaat attatttggt ttcccttcaa
     2101 aaggccgtaa aaaaaaggat tatacaccaa aagacggctc agcctatggc ggcaaaatat
     2161 ggcttaaaca tcctgtatat ggtgaatttg ctgtcgagcg ctacaagcag caaaatcgcg
     2221 gaaaatcgaa ggtgtggctg ggggatcaag tgggaagtga tgagttacta gaaaaactga
     2281 tcgcaccttt gacgaaagag ttattccagc aggtatttac tttccagcaa gagcagttga
     2341 tggaactaga tcatttgaga gaaaaagagc tacatgatgc gctgatttct ttaggcatca
     2401 cgggcagttc tcaagtattc caaaagcggc aagaatatta tcagaaagca caacaattat
     2461 tcaagaaaaa aggccaaaaa ttactaatca atcaaaaatt aaatgaatgg aaagagctgc
     2521 aagaaaaaat tcatcaaaaa caagaacagg aagcttattt tggcaagttg atccaacaag
     2581 aacaggaata cgagtcgcaa cagcagcagt tattggcaca attaaaagag gcgaacaaac
     2641 aattttcatt agccagacag caagaactta atttttcttt atatgaagaa tggcaatctc
     2701 tcaaaaaaaa cagcactaca ggtcttcctg atgagcaact tcttttagat ctggagtctt
     2761 tctccaaacg gtatcagcaa acaaatgagc gtttggaaga attagaagca acactggaaa
     2821 agcatagcgg gatggatcag cagtcagcca gatattattt ttatttagag caggagcagg
     2881 aactaaaaga tctccagcaa cttcaattta cagcggaaca attaaacgaa gaatggcaaa
     2941 gattacaaat tgagactaga gaaacaaatt cagaattgaa gattttggaa caaaggtggc
     3001 attggcagaa aaatccgtta ccggaagaat ttttatatga agaagaatgg aagcatcttc
     3061 tggctgaaga aagaaggatt gctgatctag aaagccagat ttctgtgcgt ctccaaatag
     3121 ctaaagaaca gcaagagatg attgaagcag aagtgaatga cttggaagag acctatccag
     3181 cattattaca aaatcagtca gagatagcac cagtctcttc atctaaaccg tatatgctga
     3241 tggctggcgg agcaataatc attgcaggta tatttgtacc aatgcccttg aaagtgatcc
     3301 ttttactaat tggtgcaggc atgattggct atttcttcta caaaaggcaa ccaaaaatta
     3361 aaactaaaaa tgatttctct gagataaaag gaatgtggca agaaaaacta ggacaacttg
     3421 atctagcctt agctcaatta gctgaagcag aagaaaaaca aaagagaact ggagaagaaa
     3481 aacagattct tcagcaagaa gtatatcgtt ttgcggatca tcggcactta ggaaagatgg
     3541 atacgctcaa actcatgaaa gaatatggac aggaagtcat agattatcag aaattatatg
     3601 gcgtatttga gcggaaacat aaaagagaac aggaaataca agaaaaacta cgacaaatcg
     3661 accaaaaatt tgagtttgtt aaagaatggt taccgataca agataagaca atttcagaaa
     3721 aaatggtatt gctttcccgt tttatacaag agatggaaca gatgaaattt gccagaagct
     3781 atcagcaaaa tactttattg aagcaagaaa tcaatcaatt gaaaaagaaa caaaaagtat
     3841 ggatacaaga atacagcgaa caattgactc aagcaggctt atcttatcct tcggaagtac
     3901 ctgtcttttt acaaaaagcc aaacaagccg aacagcagat gaaaagatac aatgaattat
     3961 cggaaatgct tactccacta tttcctgaag agctgacaaa agagaaatta tctgtaagac
     4021 tgcgccaact gaaagaaaag caatcccaac tccagcaaga agaaaaagaa gcaagtgaaa
     4081 atcggcaacg actccaactt caagtagaag agttgcaaag agacggcaca ttggacgaac
     4141 tttatcaaca agctagccgg caaaaagcaa aattacagga attactggag gaatggggag
     4201 cacttgaaat cgccgggaca ttcttaggtg atctttcaac agagatgagt gaaagacaat
     4261 taccccagtt gttaagagca gcaggtgatt atttaggtat tttaacaaaa ggaaattatc
     4321 agaagatatt actgaaaaat gatacactga ctttaacaga cggatataca gaatttccca
     4381 tctatgagtt atctacaggt acgaaagatc agctgattat ggcgatgaga tttgcttacc
     4441 tctcattaga agctaaccgt tcgatatgcc cgatcatcat tgatgatggc tggcttcatt
     4501 atgatcatca gcgaaagtat caattagctg aacttttagc taaatttgga gaaaagtatc
     4561 aaataatttg tttttcttct gataaagaaa tggtaagcta ttaccatgag atgaaccaaa
     4621 tcgtaaaaca attagaagga gcaaataatg aaaaaaattc gtgatttagt aatcgatgag
     4681 cagtttgaac aatttgtcct gatcaaaagt gcagatgtaa gaatcgctaa aaacggaaaa
     4741 aaatttattg cttttacttt ccaagatact tcaggtacta tcgatggtaa atactgggat
     4801 gcttcggagg aggaaatcaa taaatttaca gccggcacag tcgtttttct aaacggtaaa
     4861 agagaggttt atcaaggaaa ccctcaagta aagattcttc atatgcgctt aacaagggta
     4921 gatgagccag gagagcctag tttatacatg gaaagagccc ctttgaagaa agaagaaatg
     4981 gaagaagaaa tcaatcaatt gttgtttgag atcaccaatg cgcattggaa tcgtattgtt
     5041 cgctatctac ttaataaata tcaaaaacaa ttttttgagt ttccggcagc aaaaagaaac
     5101 catcatgcat ttgccagtgg attagcttat catactttga caatgcttca cttagggaaa
     5161 tcaatcgccg atgaatatcc tgagctgaat cgagcattgt tgtatgctgg tatcattttg
     5221 catgatttag gaaaggtgat tgaactttcc ggtcctatgt cgacagaata tacgcttgca
     5281 ggaaatttga tcggtcatct ggtattgatt gacgaagaaa taactaaagc ctgtcttgcg
     5341 ttgaaaatca gcgaagctga cgaagatgtt cttgtattac gtcatatggt tttagcacac
     5401 catggcttat tagaatatgg ctcacctgta cgtccaagaa taatggaagc agaaatatta
     5461 catcaaatcg acaatatcga tgcttcgatg cagatgatgt tgacttcgat tcgtcaaaca
     5521 gaaccaggaa aatacaccga tcggattttc ggtatggaca accgaagttt ctatgtacca
     5581 aaagatttat agcaaaaatt tatttttttc attcaaaaca aaaaaagcga gacataagtc
     5641 ttcgcttttt ttgttttctt ttattttgat gaatcagttg tttcttcagt tgaagattct
     5701 tttgttgaat cagttgattt tgtgtcagaa ctctttgttg aagctgtagt ttcacttgag
     5761 tctttcgtac ttgatgaagt tgttgtgaat gcactcaaca cattttcaaa ggcatcatct
     5821 ttgattttta catttgcatc ttttaattct tcaccgatta ctttagttgt gaatgcattg
     5881 tcacttagtt tagtttcagt agcgatatct tttagttgat ctttgtattt atccatgtca
     5941 ttgcctttat tttggttttt caccattttg acaacgtagt attctgtcgc ataagaagta
     6001 ggatttgttg tagtaataac atcggagatc tcaccgtctt tcaatttgaa agctgcttct
     6061 tttacttctg caggaatagt tgttgtcgtt gagtcgaatt taactttacc gccatcttct
     6121 tttgtctctg tatcagttga tttgtctttg gctaatttag agaaatcatc tccatcatcc
     6181 gctgattttt tcacatcttt tgcttcatct tcacttgata atttgatgat ttgtgcttct
     6241 acttctggat ggaaagattt ccaagcagtt ttcaaatcgt catctgtaat atctacgtga
     6301 gctttcaagc cagcttcaaa agctaaattg ttacgaatat attctttata agtatctttc
     6361 gtatatccag cagcttctaa ttgtgattca aaagtatcac ctaatgattt cgcttgtttg
     6421 tcgtattctg catccacttg cttatcggtt actttatcgc catatttgtt attgaatact
     6481 ttataaatga tcatccgttg tactaaggat tggttcgatg attcaagttt tgcttcatca
     6541 tagaaatcag atacagtgat cgttccgcct ttcatcgttg cgatatcttt attagtatcg
     6601 cctgaacaag ctgctagagt taaaacagcc aacgcactag tagctgccaa aataattgat
     6661 ttttttttca ttaaaatttg cactcctgta attagttatt atttttatat attaactata
     6721 ccatatccca tttcctttaa aaacgttgtc gtggtagttt caaacaaaat tcaaaatttc
     6781 atcacattcc taggaaatac gcaaactttt ctcacttata tagttaaatc tgttgagaaa
     6841 ataatcatta ttatatttgt tcaggatact ctggcagatc agcttttagt ttttctactt
     6901 gttcattgat ttgtgtgata cgtggttcgg cttggaattt aaaatcttcg atatcttttt
     6961 tgattgcttc ttggaaagca ggaatcacag attcagctgt ttcttttgtt tcgatcaatg
     7021 cgcttttaaa atgattcagg ctgttattta aatcatttgt cagagttgtt gcttcttcca
     7081 attcttcgat cagtttcttt cttgtttcat tcccgctcct gggcgccaat aacagacctc
     7141 cgacagtgcc agcagcagct ccaaataaca gtcctttgaa aaagttttta atcatcgcag
     7201 gacacctgct ttacgattgt ttcttgaata gcttttagtt cttccgatcc ataactatct
     7261 tgatgattga caaagtgaat agaaaaatca tcctctttgc tgtagcgagg gactaaatgg
     7321 atatgtgaat gaaaaacaga ttgataggct agttcacggt tgttattcaa gatattaaga
     7381 cctttcattt ctggaaaagc tttttccaat gcttgagcaa tcttaggcac acgcgaaaaa
     7441 acctctcctg caagttcggg atcatattca aaaatatctg ctacatgacg tttaggcaca
     7501 actaatgtgt gtcctttcgt tgcttgagag atgtctaaaa aagcatagac cttgtcgtct
     7561 tcatatattt tatagcttgg gatttcttga ttgatgattt tgcaaaaaat acagttttcc
     7621 atataaaaca tcctttctat aaagaataaa tacttcagct taactttacc ataagttaaa
     7681 gctattgtaa ggaaaaaaac aaaataccgt cattttcttc gtggtataat aaataaaaat
     7741 aaatggcgga ggaaaaagaa tgagtttaat tatcgatcat gtcacagggg ggtacggaca
     7801 tctccctgtg ttaaaagata tcagtttcga tgtacagcct ggagagatag tagggttgat
     7861 tggactgaac ggagcaggga aaagtacgac catcaagcac attatcggtc tgctggaagc
     7921 ttcaaaagga acgatagaaa tcaatcatct atccttaaag aaaaatacgg aagaatatcg
     7981 caaacaaatt gggtatattc cagagactcc ttctttatat gaagaattga cattgagaga
     8041 acatatcgaa gtcactgcga tggcctacga tatacctatg gatatagcaa tgaaaagagc
     8101 agaaaagttg ttggagactt ttcggttatc taatcgtctt gactggttcc cagctaattt
     8161 ttcaaaggga atgaaacaga aagtgatgat cttatgcgcc tttttgatcg agccttcttt
     8221 atatatcatc gacgaaccat ttttaggctt ggatccacta gctatcaatg cattgcttga
     8281 attgatggta gaaatgaaaa aagaaggagc cggtattttg atgtctacgc atattttagc
     8341 tactgcagaa aaatattgtg atcgttttgt ggtcttacat aacggagaga tacgagcaga
     8401 cggttctctt gagatgttga gaaaagaatt tcagctgccg gattcttctt tggacgaaat
     8461 ctatattgct ttaacaaagg agcaggcaca tgagtagttt ttttcaaaaa cgtttagcaa
     8521 gacatcaaaa gaaaatgatg aaatatatgc gttatgtatt gaacgatcat tttgttttag
     8581 tttgtctttt cttgattggc ggtgtgggat tatattattc taattggttg aaaaccctgc
     8641 agccaccgtt tgcaattgga gggctcataa tctctgtatt ttggatgctc tgtttattcg
     8701 tgggtaagat ggctactttt gcagaggcag cagatattgt ttttttactt cctaaagaaa
     8761 aagaaatgca aacctatttg gaacgagctt ttcgctattc ttgcattttt ccgttgatct
     8821 ttttgatatt ggcatgcgga ttggccatgc cattagtggt ggttagtacg gagcaatcct
     8881 tttcgatgtt tttcgtctat ctgatcatat tatggagttt gaaattcagt catctgcaaa
     8941 tcaagagagc agaattgttt cgactagatc aacaagttat tcgttattgg aaattaggct
     9001 ggtttttttc ttctctgatc tttttgtttc tatcatttta ttggctaata tgggcaggaa
     9061 tagcaggtgc attagtacag gcaagtctat attactggtt tttgtggaag caacaagaaa
     9121 aaggattgga ttgggagaaa atggtgcatg tagaagaaca taggttgcat cagatctatc
     9181 aatttatcaa tttattcaca gatgttcctg aaattacggc aaagatcaaa agaagacgct
     9241 atttggatcc tatattgact catattaaaa gagaatcaaa aaacacttat ctttacttgt
     9301 ttgcaagaag attggcaaga ggttctgatt ttagtggtct ttatttccga ttagtaatta
     9361 taggtgggtt attgattgcc ggagtagtcg acttttattt tagtctgatt atcggtttct
     9421 tatttttata tttgatcggt ttccaactgt tacctttagc gaaccagttc cgctatatgg
     9481 ttctcgttca gctttatcca atccataaga atcagaagaa gcaggcgatt cagtatctac
     9541 tcacatgggt attaattatc gcgtcggtta tttttggttt gattgctgca gtcgtattag
     9601 atgggcagca gcgatgggtt ccgctagcgg cttatttgat tgttactatg ctctttgtta
     9661 aactatatgt accaactcgt ttgaagaaga tgtcagactg aaaacaaagc tgtgtagggt
     9721 aaaaatgaga atgaaaaata gagcgttgac agtaagaatg ctgttcgttc tatttttttt
     9781 gagaaaagct ggttttttga atggcaatta aatgaaaaag aacgtatatg tgcttgacgt
     9841 gtagtgttca ggcgactaga ataataagta ctgcaaaaca aagagtaaga ggacgggaga
     9901 atgcatgatg gagtttcagt tagaccaaga gtggcggtta cgtcccatca aaggcgacac
     9961 aggaaaaaca tacataggca tgcgtgatga ggacaaagtc tttattaaac gaaacacgac
    10021 tccgatgttg gctgctcttt caagggaagg aattacacca aaacttgttt ggacaaaacg
    10081 aacggggaat ggcgatactt tgacagcaca agagtggctg gaaggtcgct tgctgactcc
    10141 taatgagatt ggtcaaagaa acgacgtgat tgatgttttg taccatttac atcattcaaa
    10201 ttctcttaaa accatgctga cccgtatcgg tggtcgtgtc atgacgccgg agaagatgct
    10261 gcaaaaatat gaaaaagaat taccggaaga actccagaaa aatcaatttc tgcaaattgt
    10321 ccatcgtttt cttcgtgagc agatcccgtc aatagataag gaaaacttat cagtagtaca
    10381 cggagatgta aaccatcgta attggttagt atgtaaaaat tatttatatc tagtcgattg
    10441 ggattcaatc atgtttgcag accctgcggt ggatattgca acgattttag gaaattatgt
    10501 gccactgtct agctggaatc agtggctgat cagttatggc atacgcccga ctgacgaaat
    10561 gcttgaaaaa tttcattggt atgcggccat gaatttgctc caagaaatca ccagatattg
    10621 caaacgtgaa gatcatcgac ggatgaacga agaaatttta caattaaaac gaattttcag
    10681 tggctgaggt ttggcccaaa ctctgcggag atttggccag acctttttaa ttagaggaaa
    10741 ggaatggaga taaataatgc gtgtccgaaa tcgtccggga gcagcggaaa tgttagcggc
    10801 tcacccgaat tttgtaatta gtgatccaac attgtggaag ggaaaatgga atgaattgtt
    10861 tgaaaacgat catccaatcc acattgagat tggtatgggg aaaggccagt tcatcactgg
    10921 aatggcaaaa gcccatcctg aaatcaatta tattggagta gaaatgcagg tcagtgttgt
    10981 ttcgattgca ctagacaagt tgatcgaaca accgcttcct aacctgaaac ttctacatgt
    11041 ggatggatca gcgttgacag agtatttcgc tgacagcgaa gtagatcaaa tctatctgaa
    11101 tttttccgat ccatggccaa aaaaacgtca tgaaaaacgg cgattgacat ataagacttt
    11161 tcttgctgta gatgaacaga ttcttcgtcc aaatggagaa atccatttca aaacagataa
    11221 tcaaggattg tttgagtatt ctctggcaag tttttctcaa tatggcatga tcctcaaaca
    11281 ggtatggtta gatttacatc aaagtcaatt tgaaggcaat attatgacgg agtatgaaga
    11341 aaaattttct tcaaaaggtc aacgtattta tcgagtagaa gctcaatttc aagataagac
    11401 caaaaagaaa agtctgtgaa actaactttc tgctttcata aaaacattaa tatccataaa
    11461 taaatctcag aaaatgaaca ttacagatgt aatgtatttt ctgggattta tttgatttaa
    11521 aatgtattta tttcatcggt ttttccacaa ggtggaatca attggaagcg agaaaatgaa
    11581 aataatcctt tgtcggtgat cttcaattcg ggtatgacag ataatgttaa gaaagaaagt
    11641 gtcaaaaaag gatcaaaggt tttagctgct cctagttgat gtgctttagt tattaattca
    11701 ttcaactgtt catagacgat cgaaaaaggt tcctgtgtca tgattccgcc tataggaaga
    11761 gaaacacaag ctatttcctg ctgtttgttg acaacggtca tcccgccacc tttttgaatc
    11821 aaaagattag cagcatacag catatcctcg tcgtttgtcc caacgacaac tagattatgc
    11881 gagtcatggg caatcgttgt cgctatcgct ccttgtttca gctgaaatcc tttcactatg
    11941 cctaatccaa tgtttcccgt ttgatggtga cgctcgatca ccgcaatctt taattggtct
    12001 ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca ctttttctac caaatcttta
    12061 gtcactagac tattcgggat aatttcgatc acatgggctt gtgaagaaga aagaagcagc
    12121 tctaacgaat gtggatttaa tggttgagcg tttagttttg gcagacaaca cgtatatgaa
    12181 tttgaatgat cagcaaataa atggtcttgg agttgcccag attgaacaac taatgttccc
    12241 ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt ctgttaagaa gaaatcagct
    12301 tgatacccag gggcaatcgc acctagtgtt ttcaaatcaa aacattctgc tgcattcaat
    12361 gttgccatct gaatggcttg taatggatcg attccaagtg caatagcttg acggacgata
    12421 tgatcgattg atccttcttt tactaaatca ttgatcagtt tatcatcggt gacaaagaag
    12481 catcttcttg aattttcagg tgtgatcgcc ggtagtagcg ataccagatc ttttgcaacg
    12541 gtcccttctc gaaccattaa atacatacca agatccaaac gttctttagc ttcttttgca
    12601 gttgtagctt catgatccgt ccgaatacct gctgcaggat aaacatttaa ctcttccatg
    12661 ccgataccag ctgcatgccc gtcaattttt ttcttatgct ggtgcatcaa ttggatcttt
    12721 tctaggatgt ctgtttcatt atttgctact gcttgatagt tcatgacttc agctaagcct
    12781 aaaacttcgg gtttttgtaa aaaaggggct aatttttttg catttaaatt tgcaccattg
    12841 gtctcaaaag gggttacagg tacacaagaa ggtagcataa aaaagatatt catgagtgtt
    12901 tgtcttgcat cttctatcat aaactcaaga cctgtactcc cagcaatgtt tcctatttca
    12961 tggggatctg tcactacagt agtaactcca tgaagcaagg atacttttga aaattcgctt
    13021 ggtgataaaa gtgagctttc gatatgcaca tggccatcga tgaatcctgg cactagatat
    13081 tgcccattag cattatgtac gctttttcct tcgtattcac cgattcctac gatcattcct
    13141 tcgcagattg ctacatcttt ttccatgatt tctttcgtaa agacgttgac gatcttagca
    13201 tgcttgatca ctaggtcagc aggctttttt ttacttgcta cttcgatata tttccttaat
    13261 gttgtttcca atggagtgcc tcccaagata aatttttata agtataacat aaattcggct
    13321 tttgccaata tttttttgta aattcatttt atttgaaaca aaaagatcaa aaagacgtat
    13381 gttttttgtg gaggggagaa taatggaaat aaaacgatta tcggatcaaa tgataagtga
    13441 aagttttgcc aaacatgccc aagacgtata tatattgcca gcttcagaag tattttacat
    13501 ctattttcgc tatggaaaaa aacgaacatt ctggaagaaa gtctcttcgg cagatggaca
    13561 gcaactgatc tcaagattta agtatttggg acagatggat atcggagaaa aaagaaaagc
    13621 tcagttaggt gcatttactt attctttaga aaaacaggaa gttcgtctgc ggttgtcttc
    13681 tgtaggagat taccttcaac gagagagttt agtgattcgg ctattatacg gtacttttga
    13741 aacaccccat cgttgcttta gttctcgaga ttttgcaact attttacaga aaaccgtcag
    13801 acgtggatta taccttttca gcggaccagt cggatcaggc aagacttctc taatgtatcg
    13861 gttggctttg gaagaaaaat tgcaagtgat tactatcgaa gatcctgtag agatagaaga
    13921 accgtttttt ctgcagctac agatcaatcc gaaaatagca cagacttatg atacactttt
    13981 gaaactcgca ttacggcatc gtccagactt gctaatcatt ggcgagataa gagatgaatc
    14041 aactgcacat gctgcggtaa gagcagcact aacagggcac cgggtttttg caaccgtaca
    14101 tgcacgcaat cttgaaggta cattggtcag aatccatgaa ctgacaaaga aaaaagaaga
    14161 gctatctgaa tgtttagctg gtgtcattta tcaagaactt ttggaagatg ccaatcacca
    14221 tccttctgtt ttatggagtt atcaatttat ggaggaagaa tcagtaaaaa aatcatggga
    14281 ggaaagttat gaagaagctc aggaaaatat ttggcagtca gaactattct aaaggattaa
    14341 caagaagcca gcaaaaccag tttgcacaaa ccatgggaga actgctttta tctggtttta
    14401 gtctgcaaga atccctttat ttattactca cgatccgttc gtttccacct gttattttgg
    14461 caaatatcca acatcagcta aaggaagggc aagcgtttag tgaggtactt tcgcaaatag
    14521 gatatggagc agagcaactg gtacagatcg agttagctga acgtcatggg aatctgccga
    14581 caactttgtt ggaaattgcc aaacagtttc ggcttgttga aggatttcgc aatgatttga
    14641 aaaagctgct tgcatatccg atgctattgc tcaccttttt gctaggtatc ttgtttagta
    14701 tgcggttgtt cgttttgcct cagcttattg gttcaggaat ggtcatgcaa gagcattggg
    14761 gtattcagct gatacaagtc tttcattggt atgttctact attcatccta gcggttactc
    14821 tattggcttt actagtaagg tatcgtctga tgaagttgtc tcctgttgat cagagtgaat
    14881 ggataagcag acaattattg attggaccga tatattcctt gtatcaatcg gcttatttgt
    14941 cactagagat tggaaagctt tttcatgaag gtttcgaatt aaggcaaatc atagtctgct
    15001 taaaggaaac gaaacaagaa agtctgattc agcatcttgc ctctaagttg attcaaggat
    15061 tggaggcagg tatgtcattg gctgagcagt tccagaatta tcgttttcta tcagaagaat
    15121 tcagtaaaat tgttttacag ggagaggcaa agggaagttt aggtaaagaa ctgatttttt
    15181 acagcgaatg gacaagaaaa cagcttttca aacgtcttca tcactggatg cattttattc
    15241 agccaatcat ttttttgggc gtagcggctt tgattttatt agtttatgcc gctgttttat
    15301 tacctgttta cggaagcata gaggaggttt tgccatgaaa aagagaaaaa tatcttatta
    15361 tagcggtttt acattgatcg aaatgctaat cgtcttactg atcatcagtg tattagtgtt
    15421 gttgttcgtc cctaatttgt cgcgttatag gaatcatgta gaccaagaaa gtcgtgaagc
    15481 gatcatccag ttggtagaca cacaaaaaga gctctatgcg cttcaaaata atggaagagt
    15541 tcctactgtg gaggaattgt tgaatgaagg ctatatcaaa cgagaacatg cagaaatcta
    15601 tcagcggcct tgaaggtaag gttttagcat caggatatac gttgtttgaa tcactcttac
    15661 tgattttatt acttacaacg atcctttctt ttccagttct cgcattttct gcatggaaga
    15721 atgaattggc agtctatcaa tttttcggcc aatttgaaaa acgaatctat tctacgcaaa
    15781 aaatcgctat tgtgaatcag atccaaacag gcttttattg gaatagtgaa gagaatcaaa
    15841 tcatttttca tgttccgaat ccagataaaa taaattggga agtattagat attcctgatg
    15901 agattacctt gaaacgccat gcttctatta cttttgctgc tagaaccggg aatgaaagtt
    15961 cactaaaagc ttatcaattt tattgggaag aaaaagagcg gacgattacg tatcaattcc
    16021 agatagggag tggtcgctat acaaaaagaa ttgaataaaa caggatttgt tttgttggag
    16081 tcccttacag cgcttatgat cgcttctgtc tgtgtctact tagtgagctt tggccagctg
    16141 tttttgcttc aggcagaaaa gaaaagtggg gaggaactgc tatttattcg aatgatttat
    16201 gaagatatca agacgcagcg gatgtatcac aaaagaccag agcgaaggga agaaatgatc
    16261 ccatacgata tccaaattgt cgataataat ggagtaatcg aaaaagtgtt tgtcaatatg
    16321 gagaaagaag cgatcagtat tgaaaaaaca agtaattcca gcatttacgc tgattgaatg
    16381 tctaattgga ctgatgatat tgagcagtct attgctcaca ttcaatcttt tgattcagca
    16441 aaccatccaa gtaagtgaat ttttgcagcg taaagatcaa aaagagtggc taatattttt
    16501 agcacaatta gaagaagaac tcaagagcag tcacaatgtc tctgtgaaaa atcagcaact
    16561 gcactacacg atcgaggata aacagtatat catcgaaaga tatcaggcaa tgatacgtaa
    16621 aagaagaact tccggcggac atcaacctat gttaacaagt atcactgagc ttcaattatt
    16681 ggaaaaagaa cacacaattt ctttttatgt acactttgaa aatggagaag atggatatgc
    16741 catatggaca aaaaacgatc agtagaaaat gttataaggg ctctatttta ttttctgtct
    16801 tgcttttatt tcttttatgt acgtttttat tgatggcagc attagaaagc taccgtttgt
    16861 caatggatct taatatacga atcaaaaatt attatatagc aaaaataatg aagcagatgg
    16921 ctttatcgga acagtctacg cttgccgaaa gttcggaagg agttttttac tacacaactg
    16981 gatctgtaac atatcagtgg gtacaacagt ccctattctt agaggtggaa gtctcaccgt
    17041 ttatctttcg gttcacagaa gaagtaaaaa atgatacaga tactagtaca gaatgatgga
    17101 atgttcgtga aaacgccctc ttttctgtta taacactgtt tttaataaaa aaatgtttgc
    17161 cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt gtcaattgtt cgatagaatt
    17221 aaacaggtga aaaaattgtt ataatatatc tagaaatgag gtgcaccatg ttaccagaaa
    17281 aaatggaaca agggtttaat caaaacctgg aagctattca gcttttacag aatgcattgg
    17341 gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa tttaatagat gattatcaag
    17401 tacgtgtggt ggatggagtg ccgacaaaag agacaacgca acggatcaca gctctttatg
    17461 aagagttgaa gaaactttct ttcgagccag aagaatggcg cagactgtct caattacttt
    17521 tattgaaggg cagccagaca gaacatttgc aggcgaacca tcaactcact cctgatagta
    17581 tcggtttttt atttgttttt cttattgaac aactatacac aaataaaaaa gcaccggtta
    17641 agattctgga tattgcggca ggaatgggca acttgttatt aacagttctt ttaaacctat
    17701 cgaatgcggg gtaccaaaca gaaggaattg gtgtggatat tgatgataca ttgttggctg
    17761 tcgcagcttc tacaagtgat ttgacacaag caaatgttca atactttcat caagacgggt
    17821 tgcaggaatt attaatagat cctgtcgatt ttgcaatcag cgatttgcca atcggttatt
    17881 atccgaatga cgaaaaagca aaagagtttt taacaagtac cgaagaaggc catagttatg
    17941 cacatcattt attgctagag caatcaatga aatatgtgaa gccggatggt tttggtttgt
    18001 ttttgatgcc gagtggattt ttagaaacgg atcaaagtga agaaatcaaa aaatggttta
    18061 aagaagaagg ttatcttcaa ggaatgattc aattgccaga tgaattgttc cgtaataaac
    18121 aatcgcaaaa aagtattttg atcttacaga aaaaaggacc acaagcacaa caagtaaaag
    18181 aagtcttgct tgtgaaactt gcttcattaa aagaaccaga gaaggtgacg gaatttttca
    18241 acgagtttaa aaactggaaa tcatctgctc tgtaatcatt caacgagaag gagagtcata
    18301 tgtctaaaac aattgcaatc aatgctggga gttcaagttt gaaatggcaa ttatatcaaa
    18361 tgccagaaga aaccgtagtt gcgaaaggga tcgtcgaacg tatcggttta caagattcta
    18421 tttttaccat caagtacggc gataatcaaa aattcgaaca aatcatggat atcgaaaacc
    18481 acgaagtagc agtaaaaatg ctgttagatc agttgattga actgcaaatt ttaagctctt
    18541 atgatgaaat caccggagta gggcaccgtg ttgttcacgg aggagaaact tatggggatt
    18601 cagtagtgat cgatgagaaa gtaatggacc gcattgctga attagcagaa tttgctcctt
    18661 tgcataatcc tgcaaactta atggggatca aagcgtttaa aaagatttta cctgacattt
    18721 tgagtgtggc tgtattcgat acatctttcc actcaacaat gccaaagcaa aattatttat
    18781 atagcttacc ggtgtcttat tatgaagatt tcaaagtaag aaaatatggc ttccatggca
    18841 caagtcatcg ttatgtgtcg gaacgtgcgg caaaattatt gaacaaaccg atcgaagaac
    18901 taaaaatcat cacttgccac ttaggaaatg gtgtatcgat cacagcagtc gatggaggta
    18961 aatccatgga tacttctatg ggattcacac cattagctgg ggtgacaatg ggtacacgtt
    19021 caggtgatat cgatccagct gttctcccat atttaatgga aaaattagag attgatattg
    19081 atgaaatgat caatgttttg aacaagaaat ctggtctgct aggcctaaca ggattgtcta
    19141 gtgatatgcg tgatcttgaa aagaactatg aaaaagaaca tattcgttta gcttatgaca
    19201 ttttcgtgga ccgcatccga aaatacatcg gcggttatgt gacaacgatg aatggtgttg
    19261 atgcaattgt ctttactgct ggtatcggcg agaatgacgg acatgtacgt agtgaaatca
    19321 tcaaaggtat gtcttggttt ggttgtgaaa tcgatccagc gttgaatgaa ttgcgtggtg
    19381 aagaagtaga tatctctaca aaagaatcaa aagtaagagt attagtgatc ccaacagatg
    19441 aggaactaat gattgcccgt gatgtggagc gtctgcgcgg ctaagccaaa gcattcgtta
    19501 ggataaaaga tgaaacgcat gcaagaggga attcttgcat gtgttttttt tgcggagaag
    19561 accgcaaaat ggtaatttaa cttcaatttc ccttcttttt tacaggatct tcttagggaa
    19621 ttttgcttgg aaaagtggtc aaaagggaaa agactggcat tttatttatt gattttcagg
    19681 aaaatcccaa tattttattt tcattcatct ttataaggga cattacatat gtaaaaagaa
    19741 aaaacatttt tagatcagcc tcgtgggctg tcgactttat tcttcgcttt atgtttagct
    19801 agaaaaccga tccgaaacat tcattgatcg aataataaaa acttgatttt ccacctgtac
    19861 tttgcctgaa tcgtgaaagc acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc
    19921 ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc
    19981 ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg
    20041 atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg
    20101 ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat
    20161 tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact
    20221 tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag
    20281 caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct
    20341 actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa
    20401 ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc
    20461 aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat
    20521 gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg
    20581 aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt
    20641 gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt
    20701 caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc
    20761 ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc
    20821 cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag
    20881 aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa
    20941 atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa
    21001 agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg
    21061 aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc
    21121 ttcaaagcga tgctgcagct cactgtaacg ggccagacac ttctctaagt ttgtatctgg
    21181 ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc
    21241 agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt
    21301 cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt
    21361 caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaagg caattttttc
    21421 accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt
    21481 aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg
    21541 ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc
    21601 cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta
    21661 aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt
    21721 ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct
    21781 gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt
    21841 ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat
    21901 tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac
    21961 atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa
    22021 tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc
    22081 atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga
    22141 aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg
    22201 tatcagattc atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc
    22261 gttttaaacc acaccagggc gactaatcgc cctggtgttc acttttttta ttttacttga
    22321 actcattgac tgttttccct ttattttaaa cattataaag accgatgagt gcttgccgcg
    22381 tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg gcaggtcgtc
    22441 tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat cagttcggac
    22501 aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac caataaataa
    22561 acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt gattatcttc
    22621 tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag aggttacatt
    22681 attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct ttgccatttc
    22741 agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag aacgtgtgtc
    22801 aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga ccgccttttt
    22861 aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt tgtattgttg
    22921 taacataagc attcactcct caagtcaatc gtattgtact cttttattgt agaacacttt
    22981 agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa atcggatact
    23041 gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa agcgttgatt
    23101 gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagcac cgtaaaattg
    23161 taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat cctgtttatt
    23221 aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta aaatcctaat
    23281 catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca agagcaaatt
    23341 gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct gtagtattga
    23401 tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca aatgataagg
    23461 catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg ttcggtcaac
    23521 acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc atatgaattt
    23581 aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag ttttttggag
    23641 gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt tcatatttcc
    23701 ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa tattgctgat
    23761 tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg ttgagtgatt
    23821 tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct ttgccctctc
    23881 gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt ggcgacaagc
    23941 aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc aatttttcag
    24001 cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa cctatatctt
    24061 cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc tcaactaaac
    24121 gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt tgaaaagccg
    24181 aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt ctttggacac
    24241 attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa ggagtcgata
    24301 gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt gcacgcgaaa
    24361 ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg cgttctttat
    24421 cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt ttgacttcaa
    24481 agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc tcagtagtcg
    24541 ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc tgttttgttt
    24601 tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt tttgcttctt
    24661 ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg cgaaatgcat
    24721 aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca ggaggtccat
    24781 atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt tttccttccc
    24841 cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg cgataagcca
    24901 atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt atatcatatt
    24961 tagcaagatg tctagaaaga acagccttct gttgttttgt tttcatagct tatccaacta
    25021 atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat agatgaattt
    25081 ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta tttggagcat
    25141 gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata cctaggtcca
    25201 gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg ggcaatgtat
    25261 gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt gaacatcatt
    25321 actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc cgccgcaatg
    25381 attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag caacggtccg
    25441 ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc tatagcagat
    25501 agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa tgctttgcct
    25561 agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt tgttgtgtcg
    25621 ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg gggttggagg
    25681 aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg aaaaacaagt
    25741 gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg agatcgttgt
    25801 tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag ggatcgtttc
    25861 gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc agaaatctgt
    25921 ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag ggttggcaaa
    25981 atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag ggcaaagcga
    26041 tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc actgggataa
    26101 tcaacaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata cttatttacc
    26161 gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag tagaaactga
    26221 aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga gtggtgtcgc
    26281 gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg agattttaga
    26341 taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt ttccggaaac
    26401 gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat tacctgagtt
    26461 tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa gcaactgggc
    26521 aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga cggttgcgcc
    26581 aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac tagatcctgg
    26641 tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag cactagaaac
    26701 agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg tgctaagtat
    26761 tgcagcaaaa tatcttggtg taaaagatgt ctatgccttt gatttagacg aagtagccgt
    26821 tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc atgtatcagc
    26881 taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga acattttagc
    26941 agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa caggcacgct
    27001 gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca tgttagaaca
    27061 agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta ttttgaaaaa
    27121 accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat caaaagacta
    27181 ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga tggaacccga
    27241 ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa tcacggagat
    27301 tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa aggaactgcc
    27361 aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt ggatcgttca
    27421 gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag cctctgtcgt
    27481 taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga ttgctcaaga
    27541 ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg aaacgaaaca
    27601 agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg aggaatcagc
    27661 aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac cagatagtcg
    27721 aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg attattttgt
    27781 agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag aaacagcacc
    27841 tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt ctttcagcta
    27901 acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt caacaatttg
    27961 tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa agcttttaaa
    28021 aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa ttatcaaaca
    28081 gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt cctgtgacaa
    28141 aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac atgggtcccg
    28201 aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat gacggacagt
    28261 ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt atattagcag
    28321 atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt gttgaagata
    28381 cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct atgttagtgg
    28441 acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag ctagcagaaa
    28501 accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg gtcaaattag
    28561 ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag caaagaagga
    28621 tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg atcagccgga
    28681 tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag tattaccgta
    28741 tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt ggtactgttg
    28801 aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat ggacggccaa
    28861 aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc aacgaaattt
    28921 atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat gctgtattag
    28981 gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat attgccatgc
    29041 caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca ggaaatccgg
    29101 tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg gttgctgcac
    29161 actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg acgaaacaat
    29221 taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct tctgaattta
    29281 tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca ccaaaaggag
    29341 acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt atccatacag
    29401 atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag cttgattaca
    29461 aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt ggcccaagtc
    29521 gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa cgtttcttca
    29581 aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc aaatgtatta
    29641 cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa gaagcactcg
    29701 atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat ggagaagtta
    29761 gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa aaaatcgagc
    29821 agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct gacaaaatga
    29881 aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta attcgtatca
    29941 gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact aaaggcagag
    30001 ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca aatgtagcag
    30061 aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag tatgatgcag
    30121 atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt caaacagtca
    30181 atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat aaaatggcga
    30241 cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc gtggataaaa
    30301 tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag gacagatatg
    30361 agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca agaaattgga
    30421 aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac gcaaaaagat
    30481 gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga acaaggaaaa
    30541 atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca attcacactg
    30601 tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg accagaaaca
    30661 gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat cacagtcgct
    30721 accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc tgtaacgatc
    30781 attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa ctatgaaaca
    30841 cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct gcaaaaagcg
    30901 cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt attttttata
    30961 ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca gatttaaaaa
    31021 aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg gacgctcccg
    31081 tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca tcagcagcaa
    31141 aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa ttagtattga
    31201 agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat caccatcgga
    31261 aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat cgtatcttag
    31321 ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta tttgatttaa
    31381 gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta tcagcgattt
    31441 ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc ggaacgttta
    31501 cttctccgat tgatcacata aaaggtgtcg agaatcgcaa aaaatcagaa aaatgggcgc
    31561 aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat gtggcattat
    31621 atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa gtggcagctg
    31681 ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg gcattgatgg
    31741 gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac ttttcgacgt
    31801 tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca atgctacctg
    31861 cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc agttgggtta
    31921 ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca gattccttta
    31981 tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa tatgagatga
    32041 ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa tggatcactc
    32101 ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc tctgtacctg
    32161 aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc cgagcgctac
    32221 tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa cagtgctgat
    32281 cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc cacgaaatgc
    32341 ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt ggacattgtc
    32401 agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg gattcttcat
    32461 gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa tggggcgaag
    32521 tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg gatttctttt
    32581 tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc ctgtcagcaa
    32641 accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa gataagggaa
    32701 taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta catcgacata
    32761 tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac cgactgctcg
    32821 gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt ttggtgtgct
    32881 ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac gttttagtgc
    32941 atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc ctttatttgg
    33001 tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt tttccttcag
    33061 tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa
    33121 attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat cttccaaacc
    33181 gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt ctgcgtagac
    33241 ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt ctagtctaaa
    33301 acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc agatatctct
    33361 tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat gaaagagcga
    33421 gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc taaagtttca
    33481 ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta cgagcgttat
    33541 tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa tttcattcac
    33601 ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa gggagagaaa
    33661 actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga atcagaaaaa
    33721 tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta taatgaaatg
    33781 cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga tacaacggat
    33841 atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt gacgcttcga
    33901 ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt cggcccagag
    33961 tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga acgccctcaa
    34021 aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc agaaaaccct
    34081 gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt ggggatcacg
    34141 caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa
    34201 gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc taaacgccgc
    34261 cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca aaaatttgtg
    34321 gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca ttttgatacc
    34381 gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa tatggttcgc
    34441 ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc aaaaatgggg
    34501 gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga actaggtggt
    34561 ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt gactatggag
    34621 gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt cgcactcgga
    34681 gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt tggtttttca
    34741 gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc agataaagcg
    34801 aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc
    34861 aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata tgaacatttt
    34921 gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa atggcacaaa
    34981 gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt acattaaaag
    35041 gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg
    35101 aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg
    35161 ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga cgcggagaaa
    35221 atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt gaaatcgtga
    35281 ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca atcagtgacg
    35341 aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca
    35401 aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat gactttctcg
    35461 atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac tacctagttc
    35521 cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa ttgtttaaac
    35581 aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg
    35641 aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa acaacgttct
    35701 taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta atgaaagaag
    35761 ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc
    35821 gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat ctaagcgata
    35881 cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga
    35941 aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg gatcagttag
    36001 gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta gaagaagacg
    36061 gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc attaaagcaa
    36121 cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg
    36181 ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt gatgaatcga
    36241 aattcaattt cttatgggta actgattggc cacaatttga attttcagaa gaagaaggtc
    36301 gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc ccgttattgg
    36361 ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac ggttatgaat
    36421 taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta ctagaaactc
    36481 taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca gctttagatt
    36541 acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca atgttattag
    36601 ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa gcagcagatg
    36661 tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg aatattgatg
    36721 taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt
    36781 ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat ataaagcggg
    36841 gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg accaagcttt
    36901 cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt tatcagccag
    36961 gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact ttgtcaaaat
    37021 cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat atacctttgt
    37081 ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt atcacagtca
    37141 ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc gatccaatca
    37201 tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg ttgagaaacg
    37261 gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt
    37321 cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc ggaattctat
    37381 ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga aaagtaactg
    37441 atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa catccagaaa
    37501 aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc aatgaagcag
    37561 aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca cgatacgaac
    37621 tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta agtattgcag
    37681 aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag tttgacaaaa
    37741 attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt ctaccatagt
    37801 agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg aaaaaaagca
    37861 tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag gacacgattc
    37921 tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta tgaaaagttg
    37981 gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac tgggtttgat
    38041 gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc aactagtatg
    38101 aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt gatcacggat
    38161 ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt ttatgtcaga
    38221 agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc ggcttatatc
    38281 actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag cagcttactg
    38341 tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg atttgctaat
    38401 gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta tttggtttgg
    38461 ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat cttttatact
    38521 gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac accaggaggc
    38581 gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg gttgggaagt
    38641 gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa
    38701 aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg cttcttcttt
    38761 ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag tctgttatgc
    38821 gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg gctgacaaat
    38881 ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca gtttccagtt
    38941 atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt gcatacttta
    39001 gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt ggagcgtccg
    39061 ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt cagcaaaccg
    39121 atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc aattgcactc
    39181 gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca actgcaggca
    39241 aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga aaacaccact
    39301 agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca agggaataag
    39361 gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc tgcaacggat
    39421 ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca attatatgcc
    39481 agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac tgttctgatt
    39541 ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat ggatgcaatc
    39601 ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac
    39661 gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct gattgattgg
    39721 tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca tccaaatcct
    39781 gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg aagttcaagt
    39841 acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac
    39901 atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca tatagattca
    39961 ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt
    40021 ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc tgcatggatc
    40081 atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc attgttatgt
    40141 tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg atctttacag
    40201 aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct ctgcagtgta
    40261 ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc
    40321 ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa
    40381 ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg
    40441 atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac ttgaaatatc
    40501 gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt gtttattagg
    40561 gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga ctaagtcaca
    40621 agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga cagtcatttg
    40681 ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac gaggttgaag
    40741 ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt
    40801 tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt gagcaagcag
    40861 cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga tatcttctgc
    40921 gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa
    40981 tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt caaacctcta
    41041 ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt tatcgattta
    41101 tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc tatgagaata
    41161 aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt acttggtggg
    41221 acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg gcaaaagaag
    41281 aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct tttccagtta
    41341 aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg aaatcgacaa
    41401 atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt aaaggcggaa
    41461 tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat caaggtccgt
    41521 tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg
    41581 acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa cgacgaacac
    41641 aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa
    41701 aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc ctgttcagtt
    41761 cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt tgcgttccaa
    41821 tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata attagaagga
    41881 cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata tcggaaaaaa
    41941 actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta tactagatat
    42001 cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg ataggagtgt
    42061 cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga atttaaagga
    42121 aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac tttcagcggg
    42181 tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag aagcatttga
    42241 accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac caattgcagc
    42301 gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag ccaaggtgat
    42361 agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta gccatttttt
    42421 tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc
    42481 gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca
    42541 agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc
    42601 tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa
    42661 ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt
    42721 gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac
    42781 tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt
    42841 ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga
    42901 aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct
    42961 aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg
    43021 atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac
    43081 cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc
    43141 tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag
    43201 acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt
    43261 gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt
    43321 tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat
    43381 ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag cagatcagtt
    43441 gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt tactacccca
    43501 aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg ttttacgtca
    43561 tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca
    43621 acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa aaaggtttgt
    43681 tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc aatggacaaa
    43741 ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa cggaacttca
    43801 ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga aaaaaagtta
    43861 tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa aagacgaaat
    43921 agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag tcgaaccaat
    43981 ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca
    44041 aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa
    44101 ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa acaaacagat
    44161 gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac aattaacaat
    44221 ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg ctatctatca
    44281 ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc aagaagatcg
    44341 tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag aaaaacaaaa
    44401 agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag caactgaaga
    44461 aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac agctgaaaag
    44521 ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag gggagccatg
    44581 tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac agccaaataa
    44641 ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg tagaaagaac
    44701 tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac aattatcaga
    44761 tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag aacacttgca
    44821 acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa caactcttgt
    44881 agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa gaatagagct
    44941 ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct acaaacagca
    45001 gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa gtttagaaca
    45061 acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac ttgaaaaaca
    45121 gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc aattgttgca
    45181 agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag aaaaaataga
    45241 tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc agtcgtattt
    45301 ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata aagaaaacca
    45361 acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc tgcgtcaatt
    45421 agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc aacagttaga
    45481 agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac aagaacaaaa
    45541 aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa gccagacgaa
    45601 attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa
    45661 gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt tacaagtagc
    45721 aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag cggataccat
    45781 tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc aggcaggtat
    45841 gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac tttctttagc
    45901 attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg atgcattgtt
    45961 tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa tggaagcttt
    46021 agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa gagaattgaa
    46081 agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa gcaaaatacg
    46141 ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc cagaaaaagt
    46201 cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg tccctgacca
    46261 agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga
    46321 tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg
    46381 taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa
    46441 aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa
    46501 aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga
    46561 ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact
    46621 ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta
    46681 tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt
    46741 tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg
    46801 aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga
    46861 taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca
    46921 gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac aattttccga
    46981 tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc
    47041 attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt
    47101 ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg
    47161 attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat
    47221 tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt
    47281 agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc
    47341 tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc atgaggtctc
    47401 gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct
    47461 attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg
    47521 ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt
    47581 cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat
    47641 cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga
    47701 gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga
    47761 aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca
    47821 acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg
    47881 attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac
    47941 gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa
    48001 tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta
    48061 tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc
    48121 ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc
    48181 gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca
    48241 agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg
    48301 agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga
    48361 ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc
    48421 attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa
    48481 tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac
    48541 aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg
    48601 gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc
    48661 tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga
    48721 aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct
    48781 gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg
    48841 ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag
    48901 agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga
    48961 gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat
    49021 ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg
    49081 aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc
    49141 tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga
    49201 agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga
    49261 aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac
    49321 tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa
    49381 aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa
    49441 aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt
    49501 tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa
    49561 gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa
    49621 agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg
    49681 tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa aatctctttt
    49741 tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg
    49801 ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt
    49861 cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag
    49921 cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta
    49981 atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct
    50041 agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg
    50101 atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt
    50161 cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc
    50221 aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac
    50281 agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc
    50341 ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa
    50401 taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat
    50461 ctctg
//
LOCUS       DANKHK010000008        49203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000008 DANKHK010000000
VERSION     DANKHK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(32..361)
                     /locus_tag="PW109_000401"
     CDS             complement(32..361)
                     /locus_tag="PW109_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2510282.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            538..1266
                     /gene="sfsA"
                     /locus_tag="PW109_000402"
     CDS             538..1266
                     /gene="sfsA"
                     /locus_tag="PW109_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2510283.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1282..2295
                     /locus_tag="PW109_000403"
     CDS             1282..2295
                     /locus_tag="PW109_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2510284.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2295..3572
                     /locus_tag="PW109_000404"
     CDS             2295..3572
                     /locus_tag="PW109_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2510285.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3635..6337
                     /locus_tag="PW109_000405"
     CDS             3635..6337
                     /locus_tag="PW109_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2510286.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6489..6806
                     /locus_tag="PW109_000406"
     CDS             6489..6806
                     /locus_tag="PW109_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2510287.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6836..7156
                     /locus_tag="PW109_000407"
     CDS             6836..7156
                     /locus_tag="PW109_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2510288.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7264..8724
                     /locus_tag="PW109_000408"
     CDS             7264..8724
                     /locus_tag="PW109_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2510289.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8792..9013
                     /locus_tag="PW109_000409"
     CDS             8792..9013
                     /locus_tag="PW109_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510290.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9044..9226
                     /locus_tag="PW109_000410"
     CDS             9044..9226
                     /locus_tag="PW109_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2510291.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9226..9639
                     /locus_tag="PW109_000411"
     CDS             9226..9639
                     /locus_tag="PW109_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2510292.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9762..10943
                     /locus_tag="PW109_000412"
     CDS             9762..10943
                     /locus_tag="PW109_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510293.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(11014..11178)
                     /locus_tag="PW109_000413"
     CDS             complement(11014..11178)
                     /locus_tag="PW109_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510294.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11332..11405
                     /locus_tag="PW109_000414"
     tRNA            11332..11405
                     /locus_tag="PW109_000414"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11366..11368,aa:Arg,seq:cct)
     gene            complement(11474..12613)
                     /locus_tag="PW109_000415"
     CDS             complement(11474..12613)
                     /locus_tag="PW109_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2510295.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12912..13547)
                     /locus_tag="PW109_000416"
     CDS             complement(12912..13547)
                     /locus_tag="PW109_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510296.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13660..14295)
                     /locus_tag="PW109_000417"
     CDS             complement(13660..14295)
                     /locus_tag="PW109_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2510297.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14329..14790)
                     /locus_tag="PW109_000418"
     CDS             complement(14329..14790)
                     /locus_tag="PW109_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2510298.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14920..15351)
                     /locus_tag="PW109_000419"
     CDS             complement(14920..15351)
                     /locus_tag="PW109_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2510299.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15369..15689)
                     /locus_tag="PW109_000420"
     CDS             complement(15369..15689)
                     /locus_tag="PW109_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2510300.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15988..16764
                     /locus_tag="PW109_000421"
     CDS             15988..16764
                     /locus_tag="PW109_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2510301.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            16779..16982
                     /locus_tag="PW109_000422"
     CDS             16779..16982
                     /locus_tag="PW109_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510302.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            16998..17336
                     /locus_tag="PW109_000423"
     CDS             16998..17336
                     /locus_tag="PW109_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2510303.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            17323..17502
                     /locus_tag="PW109_000424"
     CDS             17323..17502
                     /locus_tag="PW109_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510304.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17545..18015)
                     /locus_tag="PW109_000425"
     CDS             complement(17545..18015)
                     /locus_tag="PW109_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510305.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18102..18800
                     /locus_tag="PW109_000426"
     CDS             18102..18800
                     /locus_tag="PW109_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2510306.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18978..19319
                     /locus_tag="PW109_000427"
     CDS             18978..19319
                     /locus_tag="PW109_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510307.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19312..19983
                     /locus_tag="PW109_000428"
     CDS             19312..19983
                     /locus_tag="PW109_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2510308.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19989..20675
                     /locus_tag="PW109_000429"
     CDS             19989..20675
                     /locus_tag="PW109_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510309.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            20678..21427
                     /locus_tag="PW109_000430"
     CDS             20678..21427
                     /locus_tag="PW109_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510310.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            21439..21708
                     /locus_tag="PW109_000431"
     CDS             21439..21708
                     /locus_tag="PW109_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510311.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            21713..21877
                     /locus_tag="PW109_000432"
     CDS             21713..21877
                     /locus_tag="PW109_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510312.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            21870..22172
                     /locus_tag="PW109_000433"
     CDS             21870..22172
                     /locus_tag="PW109_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510313.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            22169..22330
                     /locus_tag="PW109_000434"
     CDS             22169..22330
                     /locus_tag="PW109_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2510314.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            22327..22632
                     /locus_tag="PW109_000435"
     CDS             22327..22632
                     /locus_tag="PW109_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510315.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            22632..22988
                     /locus_tag="PW109_000436"
     CDS             22632..22988
                     /locus_tag="PW109_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2510316.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            22975..23193
                     /locus_tag="PW109_000437"
     CDS             22975..23193
                     /locus_tag="PW109_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510317.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            23190..23609
                     /locus_tag="PW109_000438"
     CDS             23190..23609
                     /locus_tag="PW109_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2510318.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            23606..24163
                     /locus_tag="PW109_000439"
     CDS             23606..24163
                     /locus_tag="PW109_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2510319.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            24160..24456
                     /locus_tag="PW109_000440"
     CDS             24160..24456
                     /locus_tag="PW109_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510320.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            24533..24946
                     /locus_tag="PW109_000441"
     CDS             24533..24946
                     /locus_tag="PW109_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2510321.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            25255..25407
                     /locus_tag="PW109_000442"
     CDS             25255..25407
                     /locus_tag="PW109_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510322.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(25404..25679)
                     /locus_tag="PW109_000443"
     CDS             complement(25404..25679)
                     /locus_tag="PW109_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510323.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            26133..26339
                     /locus_tag="PW109_000444"
     CDS             26133..26339
                     /locus_tag="PW109_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510324.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            26535..26702
                     /locus_tag="PW109_000445"
     CDS             26535..26702
                     /locus_tag="PW109_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2510325.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            26728..27072
                     /locus_tag="PW109_000446"
     CDS             26728..27072
                     /locus_tag="PW109_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2510326.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            27077..27358
                     /locus_tag="PW109_000447"
     CDS             27077..27358
                     /locus_tag="PW109_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510327.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            27461..27775
                     /locus_tag="PW109_000448"
     CDS             27461..27775
                     /locus_tag="PW109_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2510328.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     GTAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            27753..29447
                     /locus_tag="PW109_000449"
     CDS             27753..29447
                     /locus_tag="PW109_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2510329.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            29467..30645
                     /locus_tag="PW109_000450"
     CDS             29467..30645
                     /locus_tag="PW109_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2510330.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            30608..31294
                     /locus_tag="PW109_000451"
     CDS             30608..31294
                     /locus_tag="PW109_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2510331.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            31294..32454
                     /locus_tag="PW109_000452"
     CDS             31294..32454
                     /locus_tag="PW109_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2510332.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            32464..33339
                     /locus_tag="PW109_000453"
     CDS             32464..33339
                     /locus_tag="PW109_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510333.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            33336..33647
                     /locus_tag="PW109_000454"
     CDS             33336..33647
                     /locus_tag="PW109_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510334.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            33637..33990
                     /locus_tag="PW109_000455"
     CDS             33637..33990
                     /locus_tag="PW109_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2510335.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            33980..34381
                     /locus_tag="PW109_000456"
     CDS             33980..34381
                     /locus_tag="PW109_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510336.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            34374..34778
                     /locus_tag="PW109_000457"
     CDS             34374..34778
                     /locus_tag="PW109_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510337.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            34790..35398
                     /locus_tag="PW109_000458"
     CDS             34790..35398
                     /locus_tag="PW109_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2510338.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            35418..35780
                     /locus_tag="PW109_000459"
     CDS             35418..35780
                     /locus_tag="PW109_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510339.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            35982..39413
                     /locus_tag="PW109_000460"
     CDS             35982..39413
                     /locus_tag="PW109_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2510340.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            39464..40201
                     /locus_tag="PW109_000461"
     CDS             39464..40201
                     /locus_tag="PW109_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2510341.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            40211..42502
                     /locus_tag="PW109_000462"
     CDS             40211..42502
                     /locus_tag="PW109_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2510342.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            42526..44652
                     /locus_tag="PW109_000463"
     CDS             42526..44652
                     /locus_tag="PW109_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510343.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            44669..44818
                     /locus_tag="PW109_000464"
     CDS             44669..44818
                     /locus_tag="PW109_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510344.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            44815..45261
                     /locus_tag="PW109_000465"
     CDS             44815..45261
                     /locus_tag="PW109_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510345.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            45263..45400
                     /locus_tag="PW109_000466"
     CDS             45263..45400
                     /locus_tag="PW109_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2510346.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            45438..45731
                     /locus_tag="PW109_000467"
     CDS             45438..45731
                     /locus_tag="PW109_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510347.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            45728..45952
                     /locus_tag="PW109_000468"
     CDS             45728..45952
                     /locus_tag="PW109_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2510348.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            45949..46974
                     /locus_tag="PW109_000469"
     CDS             45949..46974
                     /locus_tag="PW109_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2510349.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            47164..47237
                     /locus_tag="PW109_000470"
     tRNA            47164..47237
                     /locus_tag="PW109_000470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47198..47200,aa:Ile,seq:cat)
     gene            47914..49076
                     /locus_tag="PW109_000471"
     CDS             join(47914..48190,48190..49076)
                     /locus_tag="PW109_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2510350.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT        16947 a         7802 c        10047 g        14407 t
ORIGIN      
        1 ataaaggata tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag
       61 tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat
      121 actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact
      181 gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat
      241 cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt
      301 tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca
      361 ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct
      421 tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt
      481 ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg
      541 aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat
      601 tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa
      661 gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa
      721 accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa
      781 ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta
      841 actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt
      901 tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa
      961 aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga
     1021 gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc
     1081 gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt
     1141 cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat
     1201 gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata
     1261 caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact
     1321 catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct
     1381 agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca
     1441 acagatttga agactttttt caatattact catatggaca ccatttatat tgcttctccg
     1501 aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt
     1561 gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa
     1621 cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt
     1681 gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat
     1741 tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc
     1801 tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt
     1861 ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt
     1921 ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt
     1981 gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc
     2041 aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg
     2101 gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct
     2161 catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat
     2221 gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat
     2281 gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat
     2341 atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa
     2401 gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc
     2461 tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt
     2521 cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag
     2581 agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga
     2641 aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa
     2701 aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg
     2761 acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc
     2821 tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga
     2881 aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt
     2941 actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag
     3001 aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa
     3061 aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc
     3121 gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg
     3181 tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt
     3241 taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa
     3301 tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat
     3361 caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa
     3421 ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg
     3481 agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa
     3541 aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa
     3601 cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt
     3661 gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac
     3721 tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt
     3781 aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga
     3841 agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc
     3901 tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca
     3961 tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca
     4021 ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc
     4081 ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc
     4141 ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt
     4201 gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat
     4261 tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca
     4321 gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat
     4381 tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat
     4441 catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa
     4501 agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg
     4561 agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa
     4621 gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa
     4681 ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga
     4741 accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa
     4801 cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga
     4861 gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa
     4921 aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta
     4981 tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc
     5041 tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga
     5101 tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac
     5161 gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat
     5221 gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct
     5281 catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt
     5341 aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta
     5401 tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt
     5461 gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat
     5521 tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc
     5581 aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat
     5641 cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat
     5701 cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt
     5761 gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa
     5821 aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga
     5881 tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact
     5941 aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact
     6001 tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac
     6061 agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat
     6121 cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt
     6181 tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa
     6241 cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct
     6301 tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca
     6361 cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca
     6421 gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag
     6481 tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt
     6541 tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg
     6601 cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat
     6661 taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta
     6721 ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg
     6781 cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga
     6841 agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag
     6901 cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa
     6961 aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac
     7021 acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca
     7081 cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg
     7141 cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg
     7201 attttttatt ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa
     7261 aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc
     7321 ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca
     7381 atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt
     7441 aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc
     7501 atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt
     7561 gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg
     7621 atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca
     7681 ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca
     7741 acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg
     7801 aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt
     7861 aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct
     7921 aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat
     7981 tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt
     8041 gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt
     8101 gttcaagtat tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag
     8161 ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga
     8221 aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc
     8281 cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg
     8341 atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt
     8401 gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat
     8461 gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca
     8521 gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg
     8581 ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta
     8641 tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac
     8701 cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt
     8761 cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca
     8821 agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa
     8881 agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc
     8941 taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc
     9001 aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt
     9061 atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag
     9121 gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt
     9181 catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa
     9241 aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat
     9301 gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc
     9361 aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag
     9421 tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa
     9481 ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg
     9541 cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt
     9601 ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat
     9661 ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc
     9721 tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag
     9781 agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg
     9841 cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg
     9901 gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga
     9961 atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag
    10021 caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc
    10081 gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct
    10141 atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca
    10201 aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa
    10261 gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt
    10321 atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg
    10381 aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt
    10441 ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg
    10501 gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat
    10561 tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata
    10621 tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa
    10681 tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg
    10741 gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc
    10801 aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa
    10861 gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc
    10921 caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc
    10981 ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa
    11041 tttgaatttt ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc
    11101 tttcgtaatc ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc
    11161 ttttggctta tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt
    11221 atcatctgtt aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca
    11281 gataagcttg caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta
    11341 gctcagtagg atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag
    11401 gaacgtataa ataacccctg tatcctttgc ggtacagggg tttgttttgt atttgtgcca
    11461 aaaatgtgcc aaactagtta tcatttttat ataaactatc gaacacagta ttgatttttt
    11521 ctttatcctg atcttcaagc tctcgaacaa tatgaagata agtagacatt gtagtttcta
    11581 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
    11641 tagatgcatg tgtatgcctt agtccatgca aagtaaattg cttttcaagc cctaattctt
    11701 ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga aactaatcca ttttttaagt
    11761 tgaaaaaaac aaaattatga gggtttttga tttcaaaatt ctcgaataat tctttttgat
    11821 tgattttaaa cttttttaag agatcaatcg tatgtttatc aatggaaatt ttcctatttg
    11881 aagtttcgtt ttttgttttt ccccattcat ttaatttata attccatgtt ttattaatag
    11941 tgatgatttg atcttcgaaa tcgatatctt cccatgttaa tcctagaagt tcagcaaatc
    12001 gcattccagt ggcaccagca actaacacga gcataggaga agagtactgc gcagaaaggt
    12061 tttcttctac gagtttcatt aaagatttaa attgatcata atctaaatat ttatcttctt
    12121 ttggcttcaa tgaatctttt cctttgatta ctgcttttct agttggatca aacggaatta
    12181 atccttcttc tacggcatct ttcaacgacg ctctaatatg gttattaaac ttaataacgg
    12241 tagattttac atggtcttta gcatacttat ttaaaaagcg ttgatatccc actctatcta
    12301 aatcagaaat caataccgct ggcatatatt tctttatgtt cattaacgta tcttcgtatt
    12361 ttctataagt gataggagat actgttcctt ctttataaag ttgcatccaa tcctcaaagt
    12421 aatctgataa taatagattt ttgcgatccc ctttaaggcc tttgttcaag ttatattcta
    12481 attcgttggc ggcatctttt gcttctcctt ttgtcttaaa tcctgatttt cttatcttcg
    12541 tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg ccaactattt ccacgtttga
    12601 ctaatcttgc cataattgaa tcagctctct ttctctgata caataggaac tataaagaag
    12661 cccattgttt gggttttatt attttttgca catccttttt gcttgccggc gcaaggatgt
    12721 gctatttgtc gttttaatgg tataattgga tgtgtattag atcatccaat cagaggtgtt
    12781 tattatgatt aaagtgattt ttaaaagaat aactgaactt ccagagttta gattatttgt
    12841 tgattattgg tatgtctggt tttttattgt tgcattgctg ataggtctag atgtcatatt
    12901 agaaagaaaa cttaagtaag agttgagtta ataaattgaa ttcctaaatg agatattttg
    12961 tagcccatat tttgtttgcc gacatcacct aatactatgg agctaatcgt tttttctaac
    13021 agaccaaaat cagataatct atttaagcta ccatctatta gctcttgtga atagccagac
    13081 atgctgatat ttccagtgaa attttcgttt ccacgactca ttaagcatgc taacaattga
    13141 atctcaatcg atgttaattg attcagtaca agaagataat actcctcgtt atcccatgaa
    13201 gcgttgttaa ttcgaagtaa acaattctta taaagatttt tatacatatc tattttattt
    13261 tgtgcctttg atttttctac ctcgtcattt atcccttcga atattccaat gagttggtcc
    13321 ttgttctcta agttgaaaac atcttctgga atttgatcgc gaatgttttc taatgattcg
    13381 tttaaatttt tgtagaattt ctcgatgcgt ttgaaacgct tctcgttttt agacccaaaa
    13441 taaagtgact gcacaacgcc accaatataa ggaattgctc caataccgcc ttcaactatt
    13501 aattcaagat attctttttt ggttagtttt tcattgctat tatccatagt tacctcctat
    13561 tttgtcattt cctttacaag cattgtgact cctgatggca ctcttttgta taggttttat
    13621 tattgtgcac aatctaactc ttagtggggc gtggttcatt tattttttac ttactttact
    13681 attaatcata tcccattgag cataggattt ttctatgttc gatcctcttg aactaatttc
    13741 tcctgtaaaa gttactttat cgccctttgt aaaggtatca gcattgttta agtccttcgc
    13801 aaaaattaca tatgaaactt taggtgttgc agaaatatca ttccaacttt tatcatcgta
    13861 ctctttgtct gctataatgg ctattctagt agtcattgat tcgataacag ttccttcaaa
    13921 agtatatgtt ttaccactaa tatatttagt aaaagctgta ttttgctcag tagctggagt
    13981 aatagaataa taactatcta agaaagcacc ccagtcatga ttatttgtct ctaaaaattc
    14041 ttcaaattga gttaggcttt tattgctgct gttagatgta gtagtttgag tatcagatga
    14101 tgagctttcc tgtttgttat tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac
    14161 ggtagtaaaa ataatcccga taataaggag aatcataggt gttttcttat ttgttttttt
    14221 gataaatctt acaattaaca tgattaaacc tataataaat aaaacaatac cagtaaaacc
    14281 aaatataccg tccattaata gatcctcttt ctcttttgat attgaatatt ataaaccaag
    14341 caattctttt ttcttaaaat cgaattcttc ttgattaata atcccttcat ctaacaactc
    14401 tttatacttt ttaatttcat ctgcacttga aatttgtgtt tctttggaat tattatctaa
    14461 tatagattgt tgcttctcta tatatttttt taaattttcc atttcattcc aatatttttt
    14521 tgtaaacata acggtgtttt catctttcgt ggccgctaat atacctccgc gactttcgtt
    14581 tccgcctagc aaggtaaatt gtatataacc attactcata cctggttttt ttaattgtac
    14641 ggctgatata tttttaaagg ggatagtttt ttctccttta ataccttgat taactaaatt
    14701 tataaatcct tttctaataa ttgaaataga atcatcagtt actttaatca aggtttttcc
    14761 tggagatttt attaaaatct ctttttccat tttttcttcc tcatttctgt gatatgattt
    14821 tttttgtaga agctcatata tgaggaaaga gcccgtgttg cagcacgggc ttttttaatg
    14881 ttttgctgaa atcggttttt ttaataattc ttgatgtatt catacatatt gccttgcgta
    14941 agaatatttt ttcttaaaat agcattggca gacagcataa caagtgtatc tgagcttatt
    15001 ctgaatagaa taatactcca taaatttttc gagattaaac tgagattcat cagtcagttc
    15061 gttctcaata tagatattta ataaaattag aatagctatt ttatctgctt ctgtttcaaa
    15121 ttttgagtga aaagttgtag aagtatcgta caaaactgaa aattcaaaat gagaggcgct
    15181 gaaatgtgca agttcatgag ataaatgaaa ggcttctgca gtttcaccgt atagattttc
    15241 attcaaaaaa atgattctgg gttttggata gtagaaacca ggctctttca tctccatata
    15301 aactactttc aaattgtatt cgctcaacat ttctttcaat ttcaaataca tacaaaccat
    15361 cactccaact attcattttc ctctaaagct ttagcaattg caatcgcttt acgcattgtc
    15421 tccttagata tttcttttcc atcaaaagaa aaaacagtat cgtcttctga taaatccaca
    15481 tgtttagggg tttctctttc ttctctacct agaaggtagt ctacagagac atcgaaatag
    15541 tcagcaatcc gacttaattc atctgaatta ggagtattat ttttccactt tgctagataa
    15601 ccgttagaat atccaaaatt aatttctaac tgccgtatag atatcttttt cctcttcgcc
    15661 aagtctttta ttatttcata ggtattcatc gataaaacaa cctttctaaa tgtttacaaa
    15721 aaaagtttag aaaaatacgc agaaataatt tgactaattc tgagtaatga gctatactat
    15781 atcttgtaaa cagatttaac aactaaaaag acaacaaaaa acactattga tatgtaaatg
    15841 cagaccgcca agaaagcttt aaaatcaatg tttttatgtc ttatttaatt atgctcttag
    15901 tatagagtat tactcagata ctgtcaattg aatttagaaa aaagttgtta aatttgttta
    15961 cgaatataaa agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa
    16021 aacgaagttc ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg
    16081 agtgttttgg gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat
    16141 aagatggggt cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa
    16201 tctggtttgt acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt
    16261 tgggtaacaa gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa
    16321 aaagtagaag aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa
    16381 gaagaaagaa ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc
    16441 tcctatttag atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca
    16501 gattatggga tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa
    16561 aaagtcggta accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct
    16621 catacaactg agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg
    16681 acacagaaag ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa
    16741 atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca
    16801 ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca
    16861 ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta
    16921 aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg tacgaattgt
    16981 aaagggagga acagctaatg caatatctag aagcaaaaat cccaattcca gaaggctatg
    17041 taattatctc ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga
    17101 tgacgttgcc agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag
    17161 ttttaaagaa cccaagatat agaaatatta tcgatatcga gaaaaacaaa aatggatttg
    17221 tctattatcc agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat
    17281 ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga
    17341 gcgctagtgt tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcaggcaa
    17401 gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa
    17461 gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga
    17521 aactagaatg tatattacta ttactcagtt taattctttc actaatttct ctattgggaa
    17581 gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag taaatatcat
    17641 ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa atgcaaggta
    17701 acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt atattctatt
    17761 atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg accatctgcg
    17821 gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg ttgaatagtt
    17881 agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat taagttcaac
    17941 ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac tcaatataaa
    18001 acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac
    18061 cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt
    18121 tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc gagaaaaaac
    18181 atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat tttggacaga
    18241 tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt ttcttcatta
    18301 gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct atctacttca
    18361 aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag aatactgttc
    18421 ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat gttaaaaaag
    18481 atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc
    18541 aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga gcttatgaag
    18601 aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc
    18661 caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt
    18721 ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa cagagacttt
    18781 ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat tgctaattgc
    18841 tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca
    18901 tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca
    18961 taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat ttgattgatc
    19021 ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt
    19081 tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta actatttggg
    19141 atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat atttacgtca
    19201 tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg attgatatct
    19261 cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa
    19321 atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag
    19381 aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat
    19441 gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt ttctggaact
    19501 catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac tgggacttat
    19561 gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc tgttttagat
    19621 tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc attgaagcga
    19681 tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca aggggaagat
    19741 attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac gattctagaa
    19801 gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata tgttaatgag
    19861 cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca gttaagctat
    19921 gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt agataagaag
    19981 tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa aagttcaaag
    20041 gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta aagacgatgt
    20101 atgacggcta cgatggtgat cgagtcattg aaattcgttt tattgatcct agacgtttca
    20161 ccgtacagca acgaaacttc atctatgcgc tgataggcga tatttttatc gatacaggca
    20221 tgccaacgga cttctggaag gaattcttct actttcgttt tgaaggtgtc acagggcgca
    20281 aaataagcct gaaagacgaa tcgaatacaa ctgtgagtga tgccaatgtc ttagcaaata
    20341 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
    20401 ctgcgaatca agaatattac ttctacaaat gcatcacaaa aagagtctgc tgcatctgtg
    20461 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
    20521 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
    20581 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
    20641 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
    20701 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
    20761 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
    20821 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
    20881 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
    20941 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
    21001 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
    21061 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
    21121 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
    21181 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
    21241 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
    21301 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
    21361 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
    21421 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
    21481 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
    21541 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
    21601 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
    21661 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
    21721 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
    21781 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
    21841 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
    21901 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
    21961 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
    22021 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
    22081 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
    22141 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
    22201 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
    22261 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
    22321 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
    22381 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
    22441 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
    22501 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
    22561 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
    22621 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
    22681 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
    22741 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
    22801 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
    22861 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
    22921 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
    22981 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
    23041 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
    23101 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
    23161 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
    23221 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
    23281 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
    23341 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
    23401 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
    23461 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
    23521 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
    23581 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
    23641 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
    23701 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
    23761 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
    23821 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
    23881 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
    23941 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
    24001 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
    24061 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
    24121 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
    24181 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
    24241 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
    24301 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
    24361 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
    24421 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
    24481 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
    24541 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
    24601 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattataac
    24661 ggatatgccg aaggcaccga agcatggcaa taaggcagaa gacgcgatta ttcagatgat
    24721 ggatatagaa acggagagag atgcgattct agcggctttg atggctctta gtctgattag
    24781 tcgtcagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acagaattag
    24841 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
    24901 gtttgcagaa gcatataaac acggaaaaat aattgcttat aaataatttg gcggtttttt
    24961 ggcggaatga tggcggtttt tagacattta ccagtgttat tatggtagtg tcgaaagatt
    25021 aggaaacagg atcgacaaaa taaacgaagg gaggcaatct ccctcatcgt tttaaacttc
    25081 ttcttagata gacagcaact aattatgaag aaaaggatgt gaattcaact cctcctaaat
    25141 tcttcatcgg ttgctgttta ttttgattag acagcagcac tcacaaaact aaacatgttc
    25201 ttgtcgtctt tcaactgctg ctgtctatta atttatgtat tggagggaag aaaaatgaaa
    25261 ttatcagtag aaggcactcc agaagaaata caagaattgc tccaagctat tggtagtagc
    25321 aaggagcaaa aggtaagtgt ttctaattca gatattgacg gtatttgcaa ttgtttaaac
    25381 agagaaaaaa tatctaatcc ttactaagag aaaatatgtt cgttaatgaa tttccaattt
    25441 tcctctgtag ctctaccttg ataatttttt tgcagttcac agatgaataa ggaatcgtta
    25501 ttatcaagta ctgtatttaa tttttcacac atttgatttg gattcaaatt agttttaact
    25561 aaccaaaaag atttttgtag ctttatatat gccccaaatt cttttattat ttcaaatact
    25621 ttatcatact tttgccctgg gttattaaga tcataactaa tcatgtaggg tttatacatg
    25681 ttgtcttgca catccttgtt ttatatttca gcggaccact cgctgataaa taaaattata
    25741 cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt aaatagaaca
    25801 aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga taatctagca
    25861 tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa ttatcaaagc
    25921 aatgttattt tgttgctgtc tattgttttt taattattca cacgataact aaaggtgggt
    25981 gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat gtctatatga
    26041 ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt ttatagtgtc
    26101 actgtggcgg aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc ggcaaccatg
    26161 caaggttcga ttccttgcca gcgacattaa atgcctatga cggttacgac taccgaaaaa
    26221 agatcgttaa gaagctatac ggtgctacgt acggcaatgt agtaagtgtg ctatctgtac
    26281 accaccaagc ttcggtcact gtggcggaag tagaagacgc agcggtaaat ggcgagtagc
    26341 ctcgtgagag cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg actttaagca
    26401 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
    26461 cgtgcaagtt actattacat attagatcac tctttgagtg gtctttttat ttttgcacaa
    26521 aggaggaaac aacaatgtat agaccacaat acttagaaca gaagtatgaa gtaatcactg
    26581 ttcataaagg taatggcgaa aaagtatatg agtatagaag accaataaag agcgatacat
    26641 ataaacgaaa ggaaaacaat gaagttattc cattgtatgg caaaagaata gctaagcatt
    26701 aaataagatt gcgaaaggag acggaacatg accgaggaat tctatagatg gctattacag
    26761 ttgacaagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg caggcttaga
    26821 gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact aggtaagtat
    26881 catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga cttagcttta
    26941 gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca tggcagatat
    27001 cttacagcat tagataaaca agagaagaag atagaaagct ttgctaactt cgatgcaagt
    27061 gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata cagaaaagat
    27121 tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg ttcgctatgt
    27181 aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga atcaagtaag
    27241 agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg gatatagtat
    27301 tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg tgagataacc
    27361 cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg ctgtcaggaa
    27421 aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa acgaattgtc
    27481 tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg aaagtcagaa
    27541 aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat ggggaacggc
    27601 gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg gtaaaaagaa
    27661 tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt tggataaact
    27721 tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt tataagcata
    27781 ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa gagcaattat
    27841 tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac ttcgataagc
    27901 agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa ttaattccgt
    27961 gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat aaaacactag
    28021 tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt tttgcttctt
    28081 ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat attgatatct
    28141 atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa gtaattaaag
    28201 atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt caaaatatcg
    28261 ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat gggaagcgac
    28321 caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg
    28381 ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca actaatggtc
    28441 atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt ctttctggag
    28501 aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt atttgtcgct
    28561 tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt ccaactatta
    28621 attacaatgc agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg
    28681 ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa gatacacgat
    28741 ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa
    28801 tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc agcgttgggt
    28861 tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc cacgaatcag
    28921 cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat caaggaaaat
    28981 cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg tttcttgaaa
    29041 tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca aaaatattga
    29101 agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga tctgttacac
    29161 atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta ttttttggtg
    29221 acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt tctaatggaa
    29281 atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc
    29341 ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca gtcacaaata
    29401 gctcagtaga aaatacagga actggattta ctcctctagt attctaactt gaaaggaggt
    29461 gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc aacggttcct
    29521 ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa
    29581 acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc atatgaagat
    29641 ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa taagaaaaat
    29701 aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa tgaagtattg
    29761 atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg ggatgtcaca
    29821 gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat agtccttgat
    29881 agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca aattaggcaa
    29941 ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat gaatgtttac
    30001 aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc gcaagacaat
    30061 acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt tatggaagct
    30121 gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga tttcagcgga
    30181 aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt tgagatgaca
    30241 gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg gttatcggat
    30301 caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat tcaggatgcg
    30361 tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt acgtgtggat
    30421 acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga tgtaggcatt
    30481 agaaatggag tatttacaat caacgaagga agagaacgcg ttggaaatga tcgctctgat
    30541 aaggcgatgg cagatgaaat atttataact aaaaacaatc aacaagtatc gaaaggaggt
    30601 gaggcgaatg acgacaatga aaacatttct agcagtaaag aatgaaggcg cagtaccgca
    30661 aatttttatt cagggattta ttggttctag ttggttcttt gaagggaata ctgacaaggg
    30721 aatcaaaaat attttggata gtctaggtga tcaagaagaa attgaagtag taattaattc
    30781 aaacggtgga gacgtatttc aagggattgc tattgggaac ttacttaagt caaataaagc
    30841 aaaagttaac gttgtgatta acggcttagc cgctagtgct gcttcaatta tcgcaatggc
    30901 tggcgatact ataaaaatct acaacaatgc acaattgatg attcaccgcg cttccacata
    30961 cggagaagga aatgtcgatg acttccgtac gattgctgac caactggaat caattgataa
    31021 atcggtaaag gcttcatata aaacacgatt caatggcaca gatgaagcat tgcaagaact
    31081 tcttgaaaaa gaatcgttta tggatgcaga aacagctttg agttatggat tggtcgatga
    31141 aattatcgat gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa gcgttgaaga
    31201 aattttgaat gacgttaaag aaaaaagagc agaaaaaatt gctgcattta cagcagcatt
    31261 aaataaaaca tttggacaag gagatgtaaa ataatgacag ttaaaaattt aaaaggtgta
    31321 acagctgcaa gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga atcttttagc
    31381 gcagctatgg taagcttatc taaggaaatt caggataaaa ttttagaaga agcaacagca
    31441 aaaaatcaag atcaattagt attaatgaac cgtggtcagc gtgtattaac tacacaagaa
    31501 acaaaattct ataacgaagt agtgaaaaac gaaggttttg caggggtcga agaattagtg
    31561 ccagctactg tatttgaacg tgtatttgaa gatttagaac aatctcatcc actattgcaa
    31621 aaaattactt ttgttaacac aactggtgta acagaatgga ttgtgtcacg tggagtcaat
    31681 ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag ttttagataa tggctttgat
    31741 gtaattaaca tgaagcagtt caagctatca ggttatattc ctgtatgtaa ggcaatgctt
    31801 gatttaggtc cagtatggtt agatcgttat gtccgtactg ttttagtaga atcattgaga
    31861 attgcattag aacaagcaat tgttgatggt actggtaaag atatgccagt cggaatgatg
    31921 cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga acctattaca
    31981 gctttagatg ctgtaactat gggcggtttg atggcgcgac tatcaaaatt caatatcgaa
    32041 ggtgtgaatg atccgattta tcgtaatgtg aatccttctg atgtggtcct aattgtgaat
    32101 ccaactgatt actggtctaa agtattccca gctaagactg tactaactgc taatggagaa
    32161 tacgtacaag tattgccagt accagtttca gatttgcagt caacggctgt gccagaagga
    32221 aaagcagtta ttggggtagc ctcagattac ttcatgggtg taggatctac actaaaaatt
    32281 gaagcttcag atgaatacca ttttgttgaa gacgaacgca tttatctagc taaacaatat
    32341 gcaaacggtc aacctaaacg taacgatagt ttcattgtat tagatattag cgctttggga
    32401 actactacta caactacaaa accaacaacc acaacaacta caacacaagc gtaggtgatc
    32461 agaatgaagt atattctttg tcagccggca atcaatcggt ttaaatggga gcttgaagtt
    32521 tgtttaacta atctgaagaa actaggaatc aaagatatcg tattgctttt cagcagacac
    32581 gatgatcaga ttcctatttt ttttgagaag gaatatggtg ttgaagttca tgtgtacgat
    32641 gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg gtggaaatat
    32701 ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc ggatgtcatt
    32761 ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg gtattgtagc
    32821 gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg agaaaatatt
    32881 ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac tataaacact
    32941 aattcaggag gcgcacagtg ggttattaac cgtcctaaag cgaattattg gaaaaaggtt
    33001 tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa tatacaaatc
    33061 tggacagccg agatgtgggc acagctttgg aacatgatgt atttcaatat tggtcctaaa
    33121 gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt taaagaagta
    33181 aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt caaagggaga
    33241 tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa aaaatgctct
    33301 tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa gtgactgaag
    33361 aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa gatgagtcaa
    33421 ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt acaacacttt
    33481 ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga tacgactgga
    33541 atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt ttcattcaac
    33601 attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg tagactcgaa
    33661 gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta
    33721 ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag aaatctttcc
    33781 atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac taagaaagta
    33841 aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt tatcgtaatc
    33901 gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat ctatttttat
    33961 cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac gtttaaataa
    34021 gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac tctttcaaga
    34081 ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg aaacaggaga
    34141 aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc taactttcta
    34201 ttcagaaaat agagaagatt taacaggaga ctcactagat attatttcat tgattcaaaa
    34261 tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga accaagatcg
    34321 ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt gtcatggcta
    34381 aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg atggaacgct
    34441 attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt tcggctattg
    34501 cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga agagggcacc
    34561 aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt ttcatcatta
    34621 gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt acttctaaaa
    34681 gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat ccaattacag
    34741 aacttctgat tcgtcaattc gataaattaa ataaataggg ggaacaacaa tggctaaaac
    34801 aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt ttaattttaa
    34861 gaacgcagga aatgcaatcg caacgaattg taatggacaa ttagatggcg aaacagaaat
    34921 gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa tcaaaatcta aaccaatcaa
    34981 tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgtt tcaatgggtt
    35041 gaaacaagat ggacgtatta aaccaggcat ttactcttac ggtcctgatt ccgtaggcga
    35101 agatttctca cttgctgcag agatcgtgga tgacttcgaa gaaaatagaa agttagttgg
    35161 tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga
    35221 agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca
    35281 tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc
    35341 tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa
    35401 aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa
    35461 ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta
    35521 acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc
    35581 aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat
    35641 tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat
    35701 tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg
    35761 aaaaaaagct tcaaaagtaa aaattccaaa gctcttagtt gaaacgtggg tcgatgtcta
    35821 ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt
    35881 agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc
    35941 aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag
    36001 aagcagaagt aactttcata gctaacgatg acggattgaa atctacgtta aaagaaatca
    36061 gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga
    36121 ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat
    36181 tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgcgaaa aaatattatg
    36241 gagaaaattc caccgaagtt cagaaacttg aaagagaact gattaatcaa caaacagcgc
    36301 aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg
    36361 aaatacagac gtacgagtct acaatgcaac agttggatag cgaacaaaaa aatgttcaag
    36421 ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag
    36481 cttctgaagc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg
    36541 cggagaaaac gatagatatc ctaagacgac agttagaagc tacacagtct gaatttggcg
    36601 ctacatccac agaagcaatg cagatggaag cgaagcttaa tgatgctgaa cgtgaatttg
    36661 aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca
    36721 aaatagatat gaataattta atggaagctt ctgacgtttt aagcgacatt ggcgataagc
    36781 ttacagaatt agggaaacaa gcagtggact ctgctaacag tgtaggtagt tcccagagta
    36841 agatacaagc taattttggt ttgtctaaac aagaggctga agaattaacg aatgtagcca
    36901 gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt
    36961 tggtaaagcg taatttagga gatttaaata atcaagattt acaaaatatc acggaacaag
    37021 ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg
    37081 gcttaatggt caatttcggc ttgagtgctc aagatgcaat ggatttaatg gtttcgggta
    37141 ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat
    37201 tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag
    37261 atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt
    37321 taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt
    37381 ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag
    37441 attttagcaa tatggatggt caatacgacc aattaaataa agcttcgaca atttggtctg
    37501 cgcttggcga agataatgcg atgaaagttg tccaatcttt aactgatgtt aaccatacat
    37561 ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttgcaag
    37621 agttgaacgg gaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca
    37681 tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc
    37741 ttccacaacc agtacaggat ttcattgttg ctataggtgg tcttacagca gcatttggca
    37801 tactgttacc ggcaatacta gccgtgtcat atttatttgg tccgatgatg cttataattg
    37861 gaggaattat agctgttata gcaggcgtta ttgtggcaat taagaactgg ggtgcaatta
    37921 ctgactggtt tagtggttta tggaaaaaat ttactgattg gttgggtgat acttgggaaa
    37981 gtataaaaga cggagcctca tcagtttggg atggggttaa agaaacctgg tctggatttg
    38041 tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg
    38101 gattagttga aggagcttcc aacatctggc aaggagtcca agaaacttgg caaacattcg
    38161 ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag
    38221 acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt caagctggta
    38281 ttaatattat cgtgggtatt tttgatgtag ctggacagtt attgggcgca gcttggcaag
    38341 ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg acacaatgga
    38401 taagtatcgc ttggaatggg attgtaacta cattccatac tatatttgat ccagtagtgg
    38461 catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa
    38521 ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac
    38581 aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa
    38641 tttggaattc aatcagctct acagcatcaa atatctggaa tagtgtgact agtagtataa
    38701 gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt
    38761 ggattagcgg aatttggaac ggtatcaaaa atactgcttt gaatctttgg aatggaatta
    38821 caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca
    38881 atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacatat
    38941 gggaaggcat caaaaagact gttagcgctc caattgattg gattagagac aaaatcagtg
    39001 gcatttttga taatttgaat atttctatac cacatattcc gttaccggaa tttatcatgg
    39061 aaggcagctt taacccgcta aaaggtcaaa tcccccattt gcgtgtaaaa tggcatgcta
    39121 aaggaggtat ctttactaaa ccaactttac taggtggaat gaacggtgtc ggtgaagcag
    39181 gacctgaagc agttttacct ttgaaaagat ctgttttgca agaaattggt gatcgtatct
    39241 tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat
    39301 tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa
    39361 tcattgatag cattacaaaa attcaaaatg ataatgcttc ggcatggcgt taaacaggag
    39421 agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca
    39481 ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac
    39541 cataccttct ccaaaaagac agagagttga aacttctgtt ttaggtaggt taggtggctt
    39601 agtacaagat tactcgtttg aagatatgtc gtttacattg cactataact atttagagga
    39661 tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaacta
    39721 tgcaaagaaa ttagaattct ctgacgatcc caacgtctat tacgttatcc agactatcga
    39781 tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc
    39841 gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt
    39901 taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc
    39961 tacttcatgc cagttcatct taaatgatta tgtgtttagt ttcgaaggct tagtagcagg
    40021 aactgatgta gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga
    40081 tattttagat cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat
    40141 caatcatttt aattgcactg gtttgagcaa aatacaaatt tatcgtaacg ggttaaggta
    40201 ggtgaaatag atgatcgata atttaataac tatttacgat aaaaatgacg cgaataattt
    40261 agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac
    40321 tgttagcaat aaactaaacg gagccgagat atttcagggt acttattcaa taaacggaac
    40381 taatgcagat ttgattgtag acggacgtat tattcagtgt tatgtagatg aaaatcgagc
    40441 aaaacagcgt ctacggattt attatgcaaa aacttctgta ataggcaata cgatagaagt
    40501 aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat atgacagcgg
    40561 aacagaaaag atcactgcta ctcaggcatg gcaaaacgca aaagttttag cgaaaccagc
    40621 tatcccttcg cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga
    40681 aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca
    40741 tggtgagttt ctaaaagata ataacacatt acgtcatgaa aaaagtctag gtacggatca
    40801 taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca
    40861 aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat
    40921 cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt
    40981 ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa
    41041 agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt
    41101 agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat
    41161 gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt
    41221 ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa
    41281 aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga
    41341 tcaattgaaa aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac
    41401 tgatatgatt actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag
    41461 tcgtattttg attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa
    41521 caatgctggt attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac
    41581 gttagatggc ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat
    41641 tagaggtact acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa
    41701 aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta
    41761 tgtaagtgct gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat
    41821 tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt
    41881 tctatctttt ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga
    41941 cggtagttct ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg
    42001 taggactatg aaatttacta gtgatggtat gctaacgtta ccaggttgtt tttttggttc
    42061 atgggaagat gggaaacttg ctaggtttga acaatcaacg gtacaagtat ataaagattt
    42121 tactgttaga ggtactaaaa actcaactgt accaacagaa cattatggac aacgactatt
    42181 gaacgcttat gaaactccag aatattattt cgctgattat ggggaagccg ttacaggtga
    42241 caatggtaaa gttcgtgttg atattgaccc catgtttgct gaaacagtaa atctaagtcg
    42301 gtatatgaca catgtgacac ctacagaact agttttgtgt gctgttactc atgaagatat
    42361 tgaccatttc atcattgaaa ctagtaagcc aaacgtatta gttagatgga atttagtggc
    42421 acaccgtcta gggtatgaag atattagatt aaaagaggat acagcatatg atagcacagt
    42481 gcttgaccaa aaacgttttt aaaacgaaga caaggaggta tataaatggc tagcagttta
    42541 tataatttgg ctttagattt cagcaaagaa ttaaactaca ccaaagctat tatggctcgt
    42601 caaggtgata aagggattac ggtgacggtt aaaccgtttc taaatggctt gcagatggat
    42661 acgagtggcg gaacatttac tttaaaagga acaacaccat ctaaccgtta cgtagataat
    42721 gttgcaacta gtgtaactag tgaagaagtc acgttttctc ttgatggcac atttatgagt
    42781 gaagcaggat attataaaca ctgctacgta gaatatagaa aagacaatca aattttaaca
    42841 acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat
    42901 gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt
    42961 atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt
    43021 caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt
    43081 aacttacaag tgatgtacag taacagcatc gacttcgggg actatgatta ttcggagaac
    43141 ccgaatttaa tgccatatat tacagaacct tgggttggac ctttgttagg taatggacat
    43201 acagttaaag atagtgttaa aagggttata actcatacta aaactagaac agctaattcc
    43261 ggcgacatat tatcgcttgg cttaggtatt ccgtgtacag cggaagctaa taataggtat
    43321 cttataacca ctttacgacc atctacgaca tacacattaa gcgttactat gagtgtcggt
    43381 tcagattgga ctggagagac caatactata ggggttaggc ttagatattt aaatgaacaa
    43441 ggtggtatag aactgcccat taatgcatta ataccagcaa atgtagaacg cgataaaatg
    43501 gtcactcata catttactgg tataacaaaa gataatgtaa ctagcataac aaactgttat
    43561 gttgaaatat tctcacttaa tagtgaatat aaaggaacgg ttagtgtcag ctatgacgtt
    43621 aagttaaaag cacactaccc taacttacta gatggccctt actggttagg taaagttcct
    43681 ttaggcgaga atattgctga ccctacagtt gtctttccac acaaaactag tgaatatatg
    43741 gtttatggta gacgcaatac agaaaactat atcgctgacc aaacatatac catatcaatg
    43801 aaagcaacta aactaacagt tcaatcgttt gccgtgtata ttgctgctgg ccgcgttaaa
    43861 gtaggagaca tgaagcctac tgaggggtta gctaatacat gggagttaac ttttactgta
    43921 actaagcaac atatagatag tggggtaact aattatctgg aaatatacca atacccgtca
    43981 gctacaaaag gagctgttca aattgagtgg ctcaaactag aaaaaggcaa tactcgaacc
    44041 ccgaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa
    44101 gattatgttt gggatctagc accagaatat gtcgaagata atttggccac agatattaaa
    44161 atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat
    44221 tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcaaacaaaa
    44281 gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat
    44341 gatacagcta ctaccacatt aacagttaca aacgggaata ccggatcagc aaagctttat
    44401 cgtgaaggaa aaacagtttc tatatatttt gtggctttaa acggaaaaag cagtggtgga
    44461 aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa
    44521 ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat
    44581 ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact
    44641 tacacgattt agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta
    44701 cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt
    44761 cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa
    44821 aacatttgga aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg
    44881 cttgtatccg ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa
    44941 ccgttaacaa tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg
    45001 aacgcactag atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat
    45061 gagaacggcg atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa
    45121 caggaaaata ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg
    45181 atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca
    45241 gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg
    45301 gggctgtttc actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa
    45361 agcagataaa atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg
    45421 attcaaggag ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa
    45481 ccgctatcgt ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga
    45541 ttactgagac tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat
    45601 cgagcaaatc agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag
    45661 atacacatgc agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga
    45721 gaagaaaatg aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc
    45781 cgcactagct actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat
    45841 ctgtgttgtc atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa
    45901 tcgaacctac aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa
    45961 attactatta ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt
    46021 gcttatacta ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca
    46081 attcctaaca aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc
    46141 gccaacatga aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac
    46201 ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct
    46261 aatccttatg cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa
    46321 aaaaactatc gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca
    46381 ttgactcttg atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg
    46441 acaaattatg tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt
    46501 agcaaagaaa agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact
    46561 tctgaaaaca agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca
    46621 gatggaacaa atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat
    46681 gtggctggat ggcacattgc taactataaa tacgagtata ttttcattat ggactacaat
    46741 actgggaaag aattagctcg agtaagagct gatggaattt atagaccaga tgtaaaccaa
    46801 gcttataata cttctggaaa tgttggttat catgtatctt tcaatatgcg taattttcct
    46861 agtaagaaag tctatgtaat gatgcgggca acgaatgatc cagagggaaa cactaaaggc
    46921 ggtgcgcaag atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag
    46981 ctcctcgttg aggagcagta cataactata ttgacaacta taaaaattat tcgataaaat
    47041 agtgatgtta tcgcatatct tcactatcac ccataaatag tcacactcca agctatgcga
    47101 taacaggttt gttgccacac attctactgg ttgattgttt atggctttat gtggcaacaa
    47161 ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg tcgtaggttc
    47221 gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga atttttttga
    47281 agagtattat acaagctaaa gcttttcttc attgccactc aaatgagtgg cttttttatg
    47341 tatcctttta tggattaatg aaaggatgtt tcacatagtt atacttctgt atatttgaaa
    47401 agttttactt tgatttttaa atagaaagac atttgggtta aattgtgaga taataataaa
    47461 gaagagttta aagcgcaccc caaaccactt ccccataagt gtgttacgct ttaaactctt
    47521 ttatatttga agccattaaa aagcatacca tatttttgaa aaaaagtgag aaaaaaggct
    47581 tacaattgga gtggtagtta attagtgact tatttttgat tttatagcac tgatactata
    47641 aaatatagat atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca
    47701 caagtatggt gataaaattc gttccgggct acctttttag gtagcctact ttaatcttta
    47761 tacctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt
    47821 gcgtccggca cgtcagtcat gaatcggcta cactagacta gacagaaaaa ataaggtgtg
    47881 tagaataata aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg aagaacctat
    47941 tcaaaagaat tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa
    48001 attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa
    48061 tcaacgggct cattcaaaga aagagacaac ttaacaccag aacaagcaga attgatcgca
    48121 ctaagaaaga aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg
    48181 atattcggac gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatcagcga
    48241 tgtgcaaaat tctaaatatt tctcgtcaaa cctactatta tcaagcgaaa ccgatcgaaa
    48301 atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga aaggcttacg
    48361 gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa
    48421 tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct cattttaaag
    48481 ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg acctttacac
    48541 aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg gggaaaaagt
    48601 ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt tattcctgtg
    48661 gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg tattctttaa
    48721 cagacgtcaa gctttttcat acagaccggg gaaaggaatt tgataaccaa accattcatg
    48781 agattctgaa tggttttgga attactcgtt cattgagtag gaagggttgt ccgtatgata
    48841 atgccgttgt ggaatcaacc tataaatctg tcaaagtaga attcgtgcat caataccaat
    48901 ttgagacact ggcacagcta cgtctagaat tgtttgatta tgtgcattgg tggaactatc
    48961 tacgcttaca tggcacgttg gcgtatgaaa caccgatcca aattcgacaa cagagattgg
    49021 cgaagcgaat ccttgataat gagcgcggat ctgatacctc tggagaggca gcgtaattga
    49081 atgattgtgc ttctgccgga gaaaatcaga tccgaggatg ctcattgtca agggcaatcg
    49141 tagccataac accgcagcat tcacaacacc ttataatttt tgtcaaaaaa agtgttgcca
    49201 ttc
//
LOCUS       DANKHK010000009        47470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000009 DANKHK010000000
VERSION     DANKHK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(53..787)
                     /locus_tag="PW109_000472"
     CDS             complement(53..787)
                     /locus_tag="PW109_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2510351.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="PW109_000473"
     CDS             1114..1524
                     /locus_tag="PW109_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2510352.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="PW109_000474"
     CDS             1517..2218
                     /locus_tag="PW109_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2510353.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="PW109_000475"
     CDS             2218..3804
                     /locus_tag="PW109_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510354.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="PW109_000476"
     CDS             3823..4044
                     /locus_tag="PW109_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2510355.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="PW109_000477"
     CDS             4041..4802
                     /locus_tag="PW109_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2510356.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="PW109_000478"
     CDS             5182..5691
                     /locus_tag="PW109_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2510357.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="PW109_000479"
     CDS             complement(5766..6305)
                     /locus_tag="PW109_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2510358.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PW109_000480"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PW109_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2510359.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="PW109_000481"
     CDS             7318..8169
                     /locus_tag="PW109_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2510360.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="PW109_000482"
     CDS             8197..8832
                     /locus_tag="PW109_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2510361.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="PW109_000483"
     CDS             8852..9625
                     /locus_tag="PW109_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2510362.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="PW109_000484"
     CDS             10033..10830
                     /locus_tag="PW109_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2510363.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="PW109_000485"
     CDS             10808..11221
                     /locus_tag="PW109_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2510364.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="PW109_000486"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="PW109_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2510365.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="PW109_000487"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="PW109_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2510366.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="PW109_000488"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="PW109_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2510367.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PW109_000489"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PW109_000489"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2510368.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="PW109_000490"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="PW109_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2510369.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="PW109_000491"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="PW109_000491"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2510370.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="PW109_000492"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="PW109_000492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2510371.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="PW109_000493"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="PW109_000493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2510372.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="PW109_000494"
     CDS             21905..22483
                     /locus_tag="PW109_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2510373.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="PW109_000495"
     CDS             22535..23017
                     /locus_tag="PW109_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2510374.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="PW109_000496"
     CDS             23134..23508
                     /locus_tag="PW109_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510375.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="PW109_000497"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="PW109_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2510376.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="PW109_000498"
     CDS             25685..26275
                     /locus_tag="PW109_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2510377.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="PW109_000499"
     CDS             complement(26403..27752)
                     /locus_tag="PW109_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2510378.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="PW109_000500"
     CDS             complement(27920..28660)
                     /locus_tag="PW109_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2510379.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="PW109_000501"
     CDS             complement(28673..30265)
                     /locus_tag="PW109_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2510380.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="PW109_000502"
     CDS             complement(30833..31129)
                     /locus_tag="PW109_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510381.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="PW109_000503"
     CDS             31305..32030
                     /locus_tag="PW109_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2510382.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="PW109_000504"
     CDS             32023..32865
                     /locus_tag="PW109_000504"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2510383.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="PW109_000505"
     CDS             32868..33389
                     /locus_tag="PW109_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2510384.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="PW109_000506"
     CDS             complement(33642..34205)
                     /locus_tag="PW109_000506"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2510385.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="PW109_000507"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="PW109_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2510386.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="PW109_000508"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="PW109_000508"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2510387.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="PW109_000509"
     CDS             37549..37815
                     /locus_tag="PW109_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2510388.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37992..39950
                     /locus_tag="PW109_000510"
     CDS             37992..39950
                     /locus_tag="PW109_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2510389.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="PW109_000511"
     CDS             40304..41605
                     /locus_tag="PW109_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2510390.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="PW109_000512"
     CDS             complement(41735..43105)
                     /locus_tag="PW109_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2510391.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="PW109_000513"
     CDS             complement(43287..43610)
                     /locus_tag="PW109_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2510392.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43935..44543)
                     /locus_tag="PW109_000514"
     CDS             complement(43935..44543)
                     /locus_tag="PW109_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2510393.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44873..45589
                     /locus_tag="PW109_000515"
     CDS             44873..45589
                     /locus_tag="PW109_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2510394.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45602..46297
                     /locus_tag="PW109_000516"
     CDS             45602..46297
                     /locus_tag="PW109_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2510395.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46381..47010
                     /gene="udk"
                     /locus_tag="PW109_000517"
     CDS             46381..47010
                     /gene="udk"
                     /locus_tag="PW109_000517"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2510396.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8790 c         9876 g        13412 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agttcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacaaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcaataga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgac gaaaataaaa taacaaacaa tttgtaaagt tgacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa aatttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcgaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacgattat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttc cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatggctggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggcta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa agtataaatt tttataaagg tacgataaaa gtatgcgagg aaaagcttgg
    43801 taaattagga tttttcgttt gtaaagccat ttacaaaagt ggaagcccca tatgaaaata
    43861 aacgattaga aataaaaaag gcgtgttaaa agctgctgac ccgccgcttc taacaccacc
    43921 tgattgtttc agtattattc ttctgttgaa ccgatcactt ctggttcgcc accttcacct
    43981 gtgctttctt cttcttcact ttgagcgact acagcagcga tttgctcttc actgtcggtc
    44041 acgaatttgt aatcgtcgtt ttttggaaga tcggctactg taatagaatc acctaattcc
    44101 aagtttgtca cgtcaacttc aattcgttca ggcaatttgt ctggtgtagc agatacgact
    44161 actgtataca agttttgagc taacactcca ccagatttag cacccactga ttcgccaaca
    44221 agaacgactt ctgtttcgac ttctgtttct tccgacatgt ttacagatag aaattctgca
    44281 tgttcgaaat gattggtaaa agtgttcgtt tggatcttgt gaaccaatgt attgactttt
    44341 tgtccatcga tgtccatcgt gatgactgtg ttcgctccat gttcacgcaa caatttgctt
    44401 agtgtcaaac catcgaatga gataggggta ctttctactt tatacccata aacaacagct
    44461 ggaactttcc cttcgtgacg aagtttgttt cttaatgaac ggggacgtac ttctctttta
    44521 cttacttcta atgatactga cataaccaaa attcctcctt aaaatgtgac tgtctgcttt
    44581 ggtaacggac agattttatt taagtgaatt ctaaaggcta atctcgtgca catcatcgaa
    44641 ggcagggcgc ttaaaaaagc gcaaaaggag agatgcgcgc atctcccctt agaaagtcag
    44701 gattcaactt ttctccacca ggtcattacg ctgtgtatga gaaatccaat tcactttact
    44761 atttaaccgt aacgtgaact gttaattaag tcaaggaata actatgatga tgtatttgca
    44821 agcaagaata ttcaaggtat aattaattag tagtaataag aaaggaaaca gcatgcgttt
    44881 agataaatta atagaagaaa aattagaaac gacacgcaag gaaatgaagc ggctgtttgc
    44941 tttgaagcaa gtgatgatag atggcacagt cgaatttcga caaaatcgaa atgtagatag
    45001 tttactgcat caaatcgaag tagcaggtga acacttagaa acaaatgagt cctattatct
    45061 tctcaataaa cctgaaaaag ctgttacagc tgtatcagat ccagaaaaga cgacagtaat
    45121 cgatctgatt gcaagtgccg atcgtttcca accattatat ccagtgggaa gactagatag
    45181 agatacaaca ggactattgc tgatcacttc gaatggacaa ttgggatatg aaatgctact
    45241 tccagaaaaa aaggtttcaa agacttatca agtagttgtg aacgaaaggg tgacaccggc
    45301 agacgtaaca gcatttgaaa atggaatcgt attcgctgat gggtatcaat gtaagccggc
    45361 tgatttaaaa atactcaaag cagacaagca ccaaagtacg gttttattga cgatccaaga
    45421 aggaaaattc catcaagtca aaaagatgtt cttagcccaa ggaaagaaag tgaccgcatt
    45481 gaaacggcta agcatgggac cattggaatt agatgaacag ctaggagtag gcgagtatcg
    45541 atcgttgaca ttagcagaga tgaaaaagtt aagtatttat tttaaatagg aggtcaaaaa
    45601 gatgaaatat aaaactttat tgtttgatgt agatgataca ttgttagatt ttcaattaac
    45661 tgaagccaat gctttaagtg ctttgtttgc agaacaagga atcacattga caccagaaat
    45721 cgaaaccagc tataaggctt tgaatcatca tttatggcag gagtttgaaa aaggcagact
    45781 atcacgagaa gaaataatag gcagtcgttt tgggttactt ttccagcaat ttgggaaaac
    45841 agtagatagt gatcaaatgg aaaaacgcta ccgccactac ttgaaccaag gtcatgatct
    45901 tgttccagga agtctagaaa tcctgaaaaa ggtccatccg catgctgatc tttatattgt
    45961 gacgaacggt gtgtcgcaca cgcaacatcg gcgattaaca gatgcaggaa tgcttgatta
    46021 cttcaaggat atctttgtct cagaagctgt cggagcgcaa aagccgatga aagaattttt
    46081 tgatcatgtc ttttcccaca ttcctaattt tgataaaaag caaactgtga tcattggtga
    46141 ttctttgact tctgatatca aaggaggaaa tatggcaggg atcgatacga tttggttcaa
    46201 tagaaataaa ttaccagaaa tcgaagaaat ccagcctact tatcgaatcg attcattaga
    46261 ggaactttat tcattattgg aaattgaaaa ggattaattt tcagaaaaat atagcatttt
    46321 cgtgtttttt cgctataata gattagaatg aaaaaacgga tacgaaagag gttttagtca
    46381 atgacaaaaa gcaagccaat tatcattggt gtgacgggtg gatccggaag cggaaagaca
    46441 agtgtcagcc gtgcgatttt caacaacttc ccggaccatt ccatcatgat gcttgaacaa
    46501 gattcttatt acaaggatca gagccatttg agttttgaag aacgattgaa cacaaattac
    46561 gatcacccat ttgcctttga taatgatctg ctgattcagc atgtaggcga cttactgaac
    46621 tataaagcca ttgaaaaacc cgtgtatgac tatgtagcgc atacaagaag tcaggcaacg
    46681 atcattcagg aaccaaaaga agtgatcatt ctagaaggaa tcctgatttt agaagacgaa
    46741 cgtctgcgtg acttgatgga tatcaaagta tacgtagata cagatgatga cattcgtatc
    46801 attcgtcgta tcaaacggga tatggaagaa cggggacgca cattggactc cgtgatcgaa
    46861 caatatttga cagttgttaa accgatgtat catcaattta tcgaaccaac gaagcgctat
    46921 gcggatatca ttgttccaga aggcggagaa aaccacgttg ctattgattt gattacaaca
    46981 aaagtagcta gtttcttaaa ccataaatag tgcgcagcga aacagttgaa cggactgcga
    47041 ccgtttgaaa agcaaggatt ctgctttttg aacagtcacc agccaactgt ttttttattt
    47101 ataacaagag gttgtgaaaa cagcatcttg acctaagtat taaaaagaag aaacggaaaa
    47161 tagctgttta tattttaccg ttttttgagc taataccgaa ggtcagctga ttgagcaccg
    47221 tgaagtagag gttgtaaaaa agctgtcttt gcatcaagcg ataagagcat ccaaataaaa
    47281 cagctcctca cgtttttcgt tgggttgggc ttattatcgt agatgcaagc ttttgaacac
    47341 cgtttaacga gaggttgtaa cagaagtggt cagatgcaat aaataagaag aaacttacga
    47401 aaattatttc tcgaatttgt gtgagatttc gacttatttt tttgaagaag ctcttttgtc
    47461 gcacccccta
//
LOCUS       DANKHK010000010        46533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000010 DANKHK010000000
VERSION     DANKHK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            75..758
                     /locus_tag="PW109_000518"
     CDS             75..758
                     /locus_tag="PW109_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2510397.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            1403..2593
                     /locus_tag="PW109_000519"
     CDS             1403..2593
                     /locus_tag="PW109_000519"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2510398.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            2695..3656
                     /locus_tag="PW109_000520"
                     /pseudo
     CDS             2695..3656
                     /locus_tag="PW109_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
     gene            3725..5074
                     /locus_tag="PW109_000521"
     CDS             3725..5074
                     /locus_tag="PW109_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2510399.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPSFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(5257..5580)
                     /locus_tag="PW109_000522"
     CDS             complement(5257..5580)
                     /locus_tag="PW109_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510400.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            5709..6872
                     /locus_tag="PW109_000523"
     CDS             5709..6872
                     /locus_tag="PW109_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2510401.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            6942..8291
                     /locus_tag="PW109_000524"
     CDS             6942..8291
                     /locus_tag="PW109_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2510402.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKTKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8336..8767)
                     /locus_tag="PW109_000525"
     CDS             complement(8336..8767)
                     /locus_tag="PW109_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510403.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLV"
     gene            9155..11797
                     /gene="alaS"
                     /locus_tag="PW109_000526"
     CDS             9155..11797
                     /gene="alaS"
                     /locus_tag="PW109_000526"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2510404.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11997..12593
                     /locus_tag="PW109_000527"
     CDS             11997..12593
                     /locus_tag="PW109_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2510405.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            12715..13863
                     /locus_tag="PW109_000528"
     CDS             12715..13863
                     /locus_tag="PW109_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2510406.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(13901..14314)
                     /locus_tag="PW109_000529"
     CDS             complement(13901..14314)
                     /locus_tag="PW109_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2510407.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            14393..14824
                     /locus_tag="PW109_000530"
     CDS             14393..14824
                     /locus_tag="PW109_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2510408.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(14923..15156)
                     /locus_tag="PW109_000531"
     CDS             complement(14923..15156)
                     /locus_tag="PW109_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2510409.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            15339..17009
                     /locus_tag="PW109_000532"
     CDS             15339..17009
                     /locus_tag="PW109_000532"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2510410.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            17156..17542
                     /locus_tag="PW109_000533"
     CDS             17156..17542
                     /locus_tag="PW109_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510411.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            17529..18227
                     /locus_tag="PW109_000534"
     CDS             17529..18227
                     /locus_tag="PW109_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2510412.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            18232..18696
                     /locus_tag="PW109_000535"
     CDS             18232..18696
                     /locus_tag="PW109_000535"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2510413.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            18693..19604
                     /locus_tag="PW109_000536"
     CDS             18693..19604
                     /locus_tag="PW109_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2510414.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            19839..20378
                     /gene="pyrR"
                     /locus_tag="PW109_000537"
     CDS             19839..20378
                     /gene="pyrR"
                     /locus_tag="PW109_000537"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2510415.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            20392..21675
                     /locus_tag="PW109_000538"
     CDS             20392..21675
                     /locus_tag="PW109_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2510416.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            21688..22614
                     /locus_tag="PW109_000539"
     CDS             21688..22614
                     /locus_tag="PW109_000539"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2510417.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            22633..23913
                     /locus_tag="PW109_000540"
     CDS             22633..23913
                     /locus_tag="PW109_000540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2510418.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            23918..24994
                     /gene="carA"
                     /locus_tag="PW109_000541"
     CDS             23918..24994
                     /gene="carA"
                     /locus_tag="PW109_000541"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2510419.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24999..28184
                     /gene="carB"
                     /locus_tag="PW109_000542"
     CDS             24999..28184
                     /gene="carB"
                     /locus_tag="PW109_000542"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2510420.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            28238..29014
                     /locus_tag="PW109_000543"
     CDS             28238..29014
                     /locus_tag="PW109_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2510421.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVTGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            29011..29943
                     /locus_tag="PW109_000544"
     CDS             29011..29943
                     /locus_tag="PW109_000544"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2510422.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIETLERLIKEVREERER"
     gene            29940..30656
                     /gene="pyrF"
                     /locus_tag="PW109_000545"
     CDS             29940..30656
                     /gene="pyrF"
                     /locus_tag="PW109_000545"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL2510423.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLKVGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKEIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            30663..31292
                     /locus_tag="PW109_000546"
     CDS             30663..31292
                     /locus_tag="PW109_000546"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL2510424.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            31280..31594
                     /locus_tag="PW109_000547"
     CDS             31280..31594
                     /locus_tag="PW109_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510425.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            31632..32273
                     /locus_tag="PW109_000548"
     CDS             31632..32273
                     /locus_tag="PW109_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2510426.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            32316..33008
                     /locus_tag="PW109_000549"
     CDS             32316..33008
                     /locus_tag="PW109_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2510427.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYEKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            33005..33907
                     /locus_tag="PW109_000550"
     CDS             33005..33907
                     /locus_tag="PW109_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2510428.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLATHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELIRLLNQQS"
     gene            complement(34065..35771)
                     /gene="efbA"
                     /locus_tag="PW109_000551"
     CDS             complement(34065..35771)
                     /gene="efbA"
                     /locus_tag="PW109_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2510429.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHPLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFTKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            35961..36584
                     /locus_tag="PW109_000552"
     CDS             35961..36584
                     /locus_tag="PW109_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510430.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPDLMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            37034..38083
                     /locus_tag="PW109_000553"
     CDS             37034..38083
                     /locus_tag="PW109_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2510431.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            38080..38961
                     /locus_tag="PW109_000554"
     CDS             38080..38961
                     /locus_tag="PW109_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510432.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38964..39734
                     /locus_tag="PW109_000555"
     CDS             38964..39734
                     /locus_tag="PW109_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2510433.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            40013..40867
                     /gene="aroE"
                     /locus_tag="PW109_000556"
     CDS             40013..40867
                     /gene="aroE"
                     /locus_tag="PW109_000556"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2510434.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFKVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELLTKEKMPI
                     HVIEKLFD"
     gene            41045..42208
                     /locus_tag="PW109_000557"
     CDS             41045..42208
                     /locus_tag="PW109_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2510435.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEEATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            42325..42735
                     /locus_tag="PW109_000558"
     CDS             42325..42735
                     /locus_tag="PW109_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2510436.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFETEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            42909..45137
                     /gene="topB"
                     /locus_tag="PW109_000559"
     CDS             42909..45137
                     /gene="topB"
                     /locus_tag="PW109_000559"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2510437.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQRKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPRDKRTQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYDGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKVVQKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTAHWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            45308..45592
                     /locus_tag="PW109_000560"
     CDS             45308..45592
                     /locus_tag="PW109_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510438.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLMVVAAMIIIYFLPVITQVFHQYFG"
     gene            45752..45988
                     /locus_tag="PW109_000561"
     CDS             45752..45988
                     /locus_tag="PW109_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2510439.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFIGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45985..46383
                     /locus_tag="PW109_000562"
     CDS             45985..46383
                     /locus_tag="PW109_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2510440.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEVYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRREAV
                     DFIIMITTSSLDFDSLKSKITNYLRQTAIK"
BASE COUNT        15279 a         8068 c        10087 g        13099 t
ORIGIN      
        1 atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat ttacttaaga
       61 aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg gaccaattag
      121 cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag ttagctgaaa
      181 aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca gcttacctac
      241 atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa cagcttcgtg
      301 tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt tccattatcg
      361 aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg gaaggtaaga
      421 tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg cgtactgctt
      481 atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg caaacattca
      541 aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat gaaaaactgt
      601 tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa cgaagagaaa
      661 ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac gatgaaaatt
      721 ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga cagaagtggt
      781 cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca ctatttacca
      841 agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaatagctt
      901 ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac accgtttatt
      961 cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat tcaacaagta
     1021 aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg aagatggaaa
     1081 ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa tacaattgat
     1141 tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg tagagtaaaa
     1201 tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa gtaaaaagat
     1261 actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt tatcaaaaat
     1321 atggcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt ctgcaagaca
     1381 gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt tagagaaaga
     1441 attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag aaagtgaaaa
     1501 tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca acaattatct
     1561 tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg aaacttatgg
     1621 tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc agcaattaga
     1681 agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat ctggattcaa
     1741 ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct tatcggatga
     1801 attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa tcatccgaat
     1861 taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag aaagtaagaa
     1921 atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag acattgcacg
     1981 attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg ttgatgatgc
     2041 gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg gattaagcga
     2101 caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg ggggttatgt
     2161 agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat tcctattttc
     2221 tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt tgatgcaaga
     2281 acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag aagaattgaa
     2341 aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca ttttgggaga
     2401 cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt atgctaaacc
     2461 aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc cgactgcgga
     2521 acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag gaaaagaaat
     2581 ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa catgttcacg
     2641 catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat catcatgaaa
     2701 aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac acggctacgt
     2761 ggctacgtca agaactaggc agtgaaaatg tcattgcgac agatatacgc atgcctgaag
     2821 tcattgagaa aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca
     2881 gcatagcaga acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg
     2941 ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat
     3001 tggaagtcgc tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg
     3061 gtccaaatac tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt
     3121 atggggtaac gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg
     3181 tagatactcg tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg
     3241 gtacgacaga ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta
     3301 gtttcattga tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca
     3361 ttataaaact catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat
     3421 cggcgatgtc ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt
     3481 ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt
     3541 cactagatgt ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg
     3601 tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca
     3661 agaaccacat ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca
     3721 agggatggaa gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa
     3781 acgtacgatg ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg
     3841 ggtatttttt aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt
     3901 ttcttggttt ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc
     3961 agctgctatt ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac
     4021 agcagctttt ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc
     4081 cttgtcgatc attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat
     4141 tgttcccgta gctgttacag ctgctgtatc gatcctgctg atcaattttt taggttctaa
     4201 agctggaggg gcttttcagt cgattacgct tgtctgcaag cttattcctt cgtttgtgat
     4261 cgtcattttc ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc
     4321 aggagaaaat ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc
     4381 atacgacggt tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga
     4441 tttgccaaaa gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa
     4501 tgcggtgttc ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag
     4561 tgacgttgca aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt
     4621 gatttctgtt tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt
     4681 tatggcaaaa gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac
     4741 taaagttcct gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat
     4801 cggtggtttt gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat
     4861 ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa
     4921 agttccaatg tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc
     4981 aagcacgctt atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg
     5041 tatcccgatt tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac
     5101 tgcacttctc tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca
     5161 aaagtactcg ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt
     5221 aaaagacaga aatcgggctc aaccgatttc tgccttttac tttgatttgc gtaactgaac
     5281 tattcggcgt acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg
     5341 aagccaaatc agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt
     5401 atccatttca agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga
     5461 aataatcagg agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat
     5521 cgtagcataa aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat
     5581 ctcgacaccc cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct
     5641 cgttacttgc ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga
     5701 ggagtcatat ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt
     5761 tagccttagc gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc
     5821 tagtaacaag ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt
     5881 tggtgatctt accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg
     5941 agctgcattt atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg
     6001 aacctagtac ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg
     6061 ttttattact cataccattt ggcatttatt taagatatta tttccggctt tcttttatga
     6121 aaacgatgat tgctagtttc tgcctttctc tattttttga attgacgcag ctaagtggac
     6181 tttattttat ctacccaaga ccataccgct tatttgatgt gaatgattta gtgacaaata
     6241 cattgggtgg aatcattggt tttgtcgtta cgccgttatt tacatttatg cttcctacaa
     6301 gaagacgaat ggatgaactt tcttatgaaa aaggaaagac tgtctcattg actcgacgct
     6361 tggtcgctta tttgatcgac tggacatttt tatctcttat taatttggta gcggcgattt
     6421 tctttaaaat tgcgttcgat tcaggggcag ttacttctca tatctggtgg cttttattag
     6481 aagtaggggt atactttatg attgtaccgt ttataaccaa tggatggaca cttggcaaaa
     6541 aaattgtgcg tatccgtttg gtggaagaag accgcgaacg aatttcttta aaagcattga
     6601 tgatccgcta cgggtatctg tactttcttt tttatggttt aagctggttg gggcagttgc
     6661 ttcagcaaat catgaattcg acaacgaata cggtgttgca gatgagtgca gtattcattt
     6721 ctctcggtgt cttgttacta caaggtattt ttattttaaa tatcttgcta tcatttttga
     6781 gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac atatactgtt agcacgattg
     6841 gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa aaacgctttg tctgtgttat
     6901 aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg aatgaataat tttgatatat
     6961 tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa cagcccacag
     7021 aggtccagga acgtttgatt cctttaattg caaaaggaaa aaatatcatt gggcaatctc
     7081 aaactggtac aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa atcaatgcag
     7141 aaaaagagga agtacaagtt gttattacag ctccaagtag ggaactagct gcacagatct
     7201 atgaagcagc tacacaaatc gctaaatttt ctacaccgga gatccgtgtc tcgaattttg
     7261 tcggcggtac agacaaacag cgtcaattaa atcgtctgca tcaccaacag ccacaggttg
     7321 tagtaggcac gcctggtaga atattagaca tgatgaatga acaagcgctg aagattcaca
     7381 ccgcatttgc atttgttgtc gatgaagcgg atatgacatt ggatatgggt tttttaaatg
     7441 aagtagacca aattgcaagt cgattaccag aaaagctgca gtttttagtt ttttctgcca
     7501 ctattccaga aaaacttcgt ccattcttac gaaaatacat ggaaaatcca atcattgaag
     7561 aaatcaaacc aaaagcagtt atttctgaag acattgaaaa ttggctgatt tcaacaaaag
     7621 gtaaagatga gaatcaaatg atttatcaac tgctgacaat tggtcatcct tatttagcaa
     7681 tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc cgattacttg aaaaaccaag
     7741 gcttgaaagt agcaaagatt catggagaca tcaccccaag agagagaaaa cgagtgatgc
     7801 gacaagtaca gaatctggag tatcaatatg ttgtagcgac tgatttagct gctagaggga
     7861 tcgatatcga aggtgtctct catgttatca atgcagaaat tccagaagat ctagacttct
     7921 ttatccatcg tgtcggacgt actggacgta atggcttaaa aggaacggcc attacgttat
     7981 attcgccaaa tgatgagcaa gccattgatg cgatagaaaa attgggcgtc tcatttgaac
     8041 caaaagaaat caaaaacggg gaaatcgtca aaacatacga tcgtaataga cgtgccaaac
     8101 gagaaaaaac aaaagatgta ttagatccca cgctgattgg tcttgtgaag aagaaaaaga
     8161 aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc aattgcagaa aaaaataaaa
     8221 aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat aagaaaaatt
     8281 cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct tgtttttaaa
     8341 ctagactttt ttccattccg atacaaagta cttcatcttc aataaaaggt tctgtgcaag
     8401 tatgataccc ttgtttttca taaaaatcta cagccgtcat ttcagctact acataactag
     8461 tctgacaacc ttctgaaacc gcccgttttt cagcagcttc aagcaactga ccgcctaacc
     8521 cttttcctcg gtattctttt gctacgcaaa atcgttccgg cttgaagcaa gtaggtgttt
     8581 tcttttcata acgcaaagta gctactggtc ttttttgatc aagaaataga taataggttt
     8641 gttgcgggat atcttttccg tcgaattcta gttctgcagg aatcccttgt tctaatacaa
     8701 aaacttgata tctcaaaaag cagctagctg cgcgtatcca ttcatccgct ccaactattt
     8761 ttttcatcat attcctcctt gtcatgaata gcagagatgt attgattcct ttattcttat
     8821 cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg agatattgac
     8881 agtgatcaat agaatgcgta tactaattaa gtacgaaaag gatatgattt gttttttaag
     8941 actcacagaa aattctttct tggctgaaaa aagaatagtc actaatcaaa ttgctccctt
     9001 tttctatttt ggtagaaata ccgacgcagc tcagcgttat cgagtttaag aggtaatgat
     9061 tacacatcat tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt gatggtgttt
     9121 ttttgtattt aatttataga aagcaggtaa tgacatgaaa caattaagta gtgcacaagt
     9181 tcgacaaatg tttcttgatt tcttccaatc taaaggacat acgattgaac ctagtgcgtc
     9241 tttgatcccc gtgaacgatc caaccttatt gtggatcaac tctggtgttg caacattgaa
     9301 aaaatatttt gatggttcag ttgtgccaga aaatccaaga atcacaaatg cccaaaaatc
     9361 tattcgaaca aatgatatcg aaaatgtcgg taaaacagca cggcaccata cgatgtttga
     9421 gatgctgggt aatttttcga ttggtgacta tttcaaaaat gaagccatcc actgggcatg
     9481 ggaatttttg actgatccaa aatggatggc attcgatcca gaaaaacttt atgtaacagt
     9541 ttatccaaaa gatacagaag caaaacgcat ctggcatgaa gaagttggcc tgccactaga
     9601 tcacattatt gatgtagaag acaacttctg ggatatcggt gcaggcccat gtggtccgga
     9661 tacggaaatt ttctatgacc gtggagaagc ttataacgat ttagcagaag acgatcctga
     9721 aaactatcca ggaggagaaa atgaacgtta tttagaaatc tggaacttag tattttcaga
     9781 attcaaccat aagcctgata acacatttga accacttcct cataaaaaca ttgatacggg
     9841 gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg ccaacgaact ttgagacaga
     9901 cttgtttatg cctattatcc acgccgtaga aaagctaagc aaacaagtaa aatatggtga
     9961 aaacccagta acagatattt cttacaaagt aatcgcagat catattcgcg ccctttcatt
    10021 tgctattggc gacggtgcat tgccatcgaa tgaagggaga gggtatgtct tacgccgttt
    10081 gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt gaacaagcat tcttatatcg
    10141 tttagtccct gttgttgggg aaatcatggt aagttattat cctgaagtac ttcaacaaaa
    10201 agaatttatt gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa caatcaatga
    10261 aggtttggaa atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg ggacgactct
    10321 agcaggaaaa gacatcttca aactatacga cacatatggc ttcccggtag aattaacgga
    10381 agaggtggca gaagatgaag gactgaaagt tgatcatgct ggttttgaaa aagaaatgga
    10441 tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg gggtacaatc
    10501 tgctttattg acagatatca aagtagaaag taaattcatt ggatacacag aacaagaatc
    10561 agatagtcaa ttattggtca tcgttaaagg tgagtctctt ttgaatgaag tcgcagaagg
    10621 tgaagctcaa atgatctttg atcaaacacc tttctatgca gaaatgggtg ggcaagtagc
    10681 ggatcaagga tggatcatga atgaagtagg cgaaatcgta gcagttgtta cagatgttca
    10741 aaaagcgcct aatggtcaat ttatgcacac cgtagaagta aaagcaccaa tgagtgaagg
    10801 acaaacctat catctattga tcgatcaatc gatgcgcaat cggatcatca aaaaccatac
    10861 agcaacccat ctactgcaca aggcattgaa agaagtactt ggcgagcatg caaatcaagc
    10921 tggttcttta gtcgctcctg gacatttacg attcgacttt acccactttg gtcaaatcac
    10981 ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg aagctatccc
    11041 agttgaaaca attgaaacag atatcgatac agccaaaaac atgggtgcaa tggctttgtt
    11101 tggtgaaaaa tacggacatg acgttcgagt agtaaatatt ggcggctggt caatcgaact
    11161 gtgcggaggc acacatgtag caaatacaga aaacatcgga atcttcaaaa tcgtttctga
    11221 atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag aagcgtacga
    11281 actacttgaa gaagaagaaa aacaactaaa acaagtagca acaattgtta aatcacctca
    11341 attgaaagaa gttgtttcaa aagcggaaca attgcaacag caagtaagag agctacaaaa
    11401 ggaaaatgaa caattagcta gcaagttagc gaatcaacaa gctggtgatg tattcaaaga
    11461 tccagaaacg atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga aagatatgaa
    11521 tcagctgcgt caattagctg accaatggaa acaaaaaaat atttcagata ttcttgtttt
    11581 agcaaacgaa caagaaggaa aagtcagtct gctagcggca atgacgaaag aatcaaatga
    11641 agctggacta aaagctggcg acttgattaa agcaatcgca ccgaaagtag gcggtggcgg
    11701 tggtggacgt ccagacatgg cacaagctgg cgggaaaaat cctgctggta ttccagatgc
    11761 actaaaagct gcaaaagaat gggttgcgca acaatagggc gtttacgttt gacagaaaaa
    11821 caaaagttca aacattgaac gaaacatgat tatgggggcg aagacaaaca agtcttgccc
    11881 tcatactttt ttcaattggc tggcgcatct aatcaaatgg tcggattcgg atggtcttta
    11941 gttgacgaaa gtgacagaaa tttttacgat tagtataaat aaaaaggggt gaaaaaatga
    12001 atagtagaaa taagaccttt cgactagtct tacgtgcgat cttgttagca atcatcatcg
    12061 tacaagcgat ggttccttgg ctggggttta ttccattagg attcattagt ttaacaatta
    12121 tccatatcac agtaattatt gctgcagtag tattaggccc aaaagatggc atggtgattg
    12181 gcttgttttg gggaatcgct acaattgttc gtgcctatgc catgccaaca acgccatttg
    12241 atacattagt gttcacgaat ccgattatct cggtagttcc cagagtactt gttggcttag
    12301 tagcaggctt ggttttccac tggatttatc aaaaaaataa atcaataacg atcagctcag
    12361 cctttgcagg ggtactgggt tcattggtca atacagtctt agtgttagga ttcatgggat
    12421 tattttatac aggtgcaacg gcagatgcct atggtgtaga ttctgctctt ttatttaaaa
    12481 ccttagcagg ggttgcagca atcaatggga taccagaagc aataggtgcg ggaatcataa
    12541 caccattact agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa taatgaccat
    12601 cttttaatag taataggacg cacgatagat agaaaaagga agagaggaga ggaaaacaat
    12661 cgtcctctcc atctttttac ctgagcaaca agcaaactag aaggggtaaa aaacttggct
    12721 aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa aggagtacta
    12781 gcgggtatct taggattggt tacgaatctg atcttgtttg tctctaagct aatgatcggg
    12841 cttttttcag gaagtgtctc cattatggct gatgcagtga acagtctatc agatactgct
    12901 tcatccatat tgactttgat cggatttaaa attgctgcaa agccagcaga tcaggaacat
    12961 ccttatggac acgaacgctt cgaatatatc agtgggttat ttgtttcgat catcattact
    13021 tatgtaggat ttcaattttt agagtcttct gctgaaaaaa tcatccatcc agaaaatgtc
    13081 tctttaacac ccatcgtctt ttttgtccta atcatttcca tctttttgaa atttgcacaa
    13141 ggcaaaatgt atcaggttat tgcgcaatcc attgattctg aaacattgag agctacagca
    13201 acagatagtt ataatgatgt ctttactaca ctggctgtat tgctttcagc agcaatcgaa
    13261 tgggtaactg gatggcgtat tgatggatat ataggttttg ctttagcatt atatatcctt
    13321 tatagtggat tgacgatgct tcgaaatttt gtatacgaac tattaggaag tcgcccaacg
    13381 ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat acttggctat
    13441 catgatttat tggtgcataa ctatggaccc aataaacgct ttgcatcagt ccacatcgaa
    13501 gtagatgaat caatgaattt gaatgtggct cataagatta ttgatctgat agaaaaagac
    13561 tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc tgttgataac
    13621 gaaaaatact ataatatctt gcggcagtta aagcaaattc ttcttgaaac aggtacaggg
    13681 cttaatattc atgattttag agtaattcat caaggaaaaa cgctgcaatt tgatgtagtg
    13741 gtccctgaga aattccagtt tgttgataca gaactagaaa gaatcttacg tagtcaaata
    13801 gaagaaaaaa tcggagatta ccaattggaa attacgtttg atcacaatta tcttttatat
    13861 taaatgagaa actataaaaa gactccttaa agtttttaat ttaaagagtc tttttttctt
    13921 ttaattgttt atggagagcc tgacgtgcaa gattatctgc tcctttattt ttgttttcgg
    13981 gcaaccattg caggatcaat aaagaaaatt cggatagaag ttcttggatt ctttgaaggt
    14041 agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact agtgttttgc
    14101 tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt tttctttaga aattctaaag
    14161 tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc tatagaaagc gggatagata
    14221 gctgttcttg tttaccttga tagacaatca agattccacc tccactagga ccagggttgc
    14281 cttttgtaga ggcatcaatg tatactttta acataaaaag agattctcct ttttgttcaa
    14341 actaatgtta tggtaagatt atatcattac tgaatcggtg agaggttaga gaatgaaaaa
    14401 aaatattcga tggcagcctg aactggcaca tacgatcatc tattggtcat tgaccttgat
    14461 cattctgttt tttagtctga ttctttcttt agaaaacaca ggtccttatt ggaaaagcaa
    14521 ttttgtttta ggtatttttc ttgtttttct atttctaggt ttgcggcgtt cttttatttt
    14581 aaaatcaaac tctgtgatta tacgttatgc tgctttttgg aaagaccgag aaatatctgt
    14641 cggacagatt cgatctttga cgccaactaa tactggggtg caaatgatgt tgcaagatga
    14701 gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt ttgatcattt
    14761 tgaaaaaaac ggacccaaat cagaatcaat tccttatcct tatacgaaaa aaagaaaaag
    14821 ctaattttaa aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa aagagtgaga
    14881 cgtacggttc atcccgttta gcgtctcact ctatttgttt atctattcat gcatactagt
    14941 atcggtagtc tgaacgttgc ctttcttgac atgggcagct tgtgttcctc gattcccttc
    15001 gatgatctca aattcgacca cttgattttc ttccaaagtt ttgaatccct cttcttcaat
    15061 cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc cgtatccttt
    15121 tttgttgtca aaccatttta caattcctgt ggtcaaagca ctcacgacct ttctaaagtc
    15181 tgatcatagc cttcattata aagagtattt ataagtacgt caaataaatt aaaaacctgt
    15241 aattttatgt tttttttctg aataaaaaaa ggtataattg gcatatctta acaaagaaga
    15301 tattttgttg aataaagttc gtgaaaaggg gtaatcaaat gaaaaatgat ctgcaaattt
    15361 cacaagaaac agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag
    15421 acgatttgga actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat
    15481 caaaggataa cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg
    15541 gcgaaggaaa gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa
    15601 aatctgttat tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg
    15661 ctgcaggcgg aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg
    15721 gtgacatgca tgctatcaca acagctaata atgcattatc tgctctttta gataatcata
    15781 ttcatcaagg aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg
    15841 atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg
    15901 ttccaagaga agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc
    15961 ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat
    16021 ttgaccgtca gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc
    16081 tgaaagatgc aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc
    16141 acgggggacc gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag
    16201 cattgagatt agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag
    16261 agaaattttt agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg
    16321 tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg
    16381 agaatcttga tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg
    16441 aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg
    16501 aactcacact gttggagcac ttgtgtgaaa atcaggggat tcttgccaag agagcgagtg
    16561 tatgggcaga tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg
    16621 atagaaaaga agctaattac aagccattat accgcctaga agaaacgata caagaaaaga
    16681 cagaaacaat cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc
    16741 aaaaacaaat agaagagttt acgaagaatg gatgggacaa actgccgatt tgtatggcaa
    16801 aaacacagta ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca
    16861 tcacgatccg cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag
    16921 atgttatgac gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa
    16981 atgacggaga agtgttggga ttgttttaag cttgattata atctaccatc gaaggagctg
    17041 tattttgact acagcttctt tttttagacg atagggaatt aattttgttt acactaggat
    17101 cattcttttt ctttattgta ttttgagcta tactaaagca taataggagg gtatgatgaa
    17161 aacgacattt ataagaaaaa ccggttttta cggtatgggt agctcgattc agctaagaat
    17221 caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg atctggaagt
    17281 gccctttacg atgcaggtca ccttttattg gctaaaaagc cccgtttata cagtgaaaga
    17341 agcaaagcct gcttatgtga tcacaatgaa ttacttgatt cttcaaatct atccgttttt
    17401 atttctagca actgggttgg gggccgtttt cctacaaaat ttatttttca gtttttttgc
    17461 agttattgtg ttgattgcgt tctttttcta tataaaaaac cgggcatatt tgatcaagga
    17521 ggaaaattat ggcaaactct gatctttttt taatcagttt caatcgaatc gaaaaatgga
    17581 tgagagacga gatgggaaat gcacgaaata tgggatttac cgaacttgtc agacgcctag
    17641 ctcaaagaaa acaattaatg atcagaaaat acgaagatga tcttttacag cttgctcagt
    17701 taaggaatgc gatcgtccat gatcggatcg cagtcgattt tgtcattgca gagccaaatg
    17761 attggacagt caagcgaatc cagctgatcg aacaagaact gattcagcct gaaaccgtgc
    17821 tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta cttgaactgt
    17881 taaagatagt agcagaaaaa aggtattcac aatttccgtt atacaataaa ggaaaatttg
    17941 tagcattgat tactttacgt aatatcgggt tttggctagc aaaagaaagc caaaaaggtc
    18001 cggttgattt aacaaataaa cgagcgttgg atttgatcat ccaaaacgga aagtacacaa
    18061 attatcattt tgttccagct aaaacacata tttacgaggt tgaagcgatg tttcgtgagc
    18121 aagggacttt ggaagccgta cttatcacta aagatgggaa tcccgatggt aatctgctcg
    18181 ggatcgtacg acctcgtgat atttataaac aaatagaaaa ggattgattt tttgttaatt
    18241 atttattgga tattcagcgc tttgctagta ggagtggatc agttggtcaa atggtggatc
    18301 actgataatc tagcgcttgg agagacgaaa aatcttatac caggtatttt gtcactgaat
    18361 catattcgaa acactggagc cgcatggagt atgctagaag ggaagatgtg gttctttact
    18421 atagtgacac tcattgcagt cgttgtgatt ctcacattga tgattaaaaa tcgtgaaaaa
    18481 ggtaatcgct ggttcatgat tggattgagt ttgattctag ctggtgcgat tggcaatttt
    18541 atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc agacagattt tatgagcttt
    18601 ccgattttta acgtagcaga tgtgacactt gtatgtggag tgatttgtat attaatttat
    18661 attattttag atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg acgattcacc
    18721 atgaatctgg taggatcgat aaagtattga ctaatttatt caccgattac agtcgttcgc
    18781 aaattcagct gtggctgaaa aatggagcgg tttctgtcaa tggacagcca gtcaaagcaa
    18841 attacaaagt aaaacaagga gacgagatta ctgtcttgat ccccgaacca gaaacattag
    18901 atgtggtccc agaaaatatc ccgttgacga tcgtttacga agatgaagat gtagcggtga
    18961 taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat ggcacaatgg
    19021 tcaatgcgct gatgtaccat atcaaggatc tttctacgat caacggcgtt attcgtccag
    19081 gtattgttca ccgaatcgac aaagatactt ccggactgct aatggttgcc aaaaatgacc
    19141 aggcacacga atcattagct cagcaattaa aagataagac ctctttgcgc aaatatgttg
    19201 ctttagtcca tggagagatt cctcacgaaa aaggaaccat caatgcgcca atcggccgtt
    19261 cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg gaagtcggct gtcacacatt
    19321 ttactgtatt ggaacgtttc gaaggattta ctctggttga attaaaacta gaaaccggaa
    19381 gaacacacca gatccgtgtg catatgaagt atatcggtta tccgttagct ggagatccag
    19441 tatacggacc gaagaaaact ttgaaaggca atggtcagtt tctccatgcg caaatgttag
    19501 gttttacgca cccgactaca aacgaaaaga tgatctttga agcacctctt ccagaagttt
    19561 ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga taacggtata
    19621 gtaattaaca acgaaagcat aaaacttaat agaattcctt taagtttagt cccgtgaggc
    19681 taagaagggt gggagcaagg atacgtatgg tgcgccataa gtagattgct cattgacata
    19741 gtatagctat cagtgtatca gtatacgctc agtctaaaca ttcctcctgt cctcttaggg
    19801 caggagtttt tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa gaagtagtag
    19861 atcaggtaac catgaagcga gcattgactc gaattactta tgagattatc gagagaaacc
    19921 atagtatcca agatatcgta ttggtaggta tcaaaacacg aggaatctac attgcatcaa
    19981 gaattgccga acgcctgaag cagctagaag atatcgatat tcctgtagga gaactggata
    20041 tcactttata ccgagatgac aaaaaagaaa atccagaaga accggaactt cattcttctg
    20101 acatcccagt ttccttagaa ggaaaagaag ttattttgat tgatgatgtt ttgtatacag
    20161 gacgaacgat tcgagcagca atggacgcag taatggattt tggcagacca agaaaaattt
    20221 cattagcagt cctggtcgat cgtgggcatc gagaattgcc aatccgtgca gattatgttg
    20281 ggaagaacat tccaacatct cgggcggaag agattttagt agagatgcaa gagcttgatg
    20341 gacaagatcg catcatgatt ttgaaagagg aagattagag gagagagaaa catgtcggag
    20401 aaaaaaggat ttcataatga tgacgtggtg ttagatatcc acgatcgccc aaccccagga
    20461 cattgggtag gattaagttt acaacacttg ttcacgatgt tcggagcaac cgtgttagtc
    20521 cccatcttag taggtatcga tccaggtatt gcgctagtca gttcaggact aggtacattg
    20581 gtgtacttat ttactactaa aggaaagata cctgcgtact taggaagcag cttcgctttc
    20641 attgcagcga tgcagctttt gatgaaaaca gatggttatc cagcgattgc acaaggagca
    20701 gtcacaactg gattagtcta tttgattgtt gcgctgatca tcaaaaagat cggttcagca
    20761 tggctggata aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt tatcgggttg
    20821 ggacttgcgg caaatgcagc aacaaatgct atgtataatc agggcacgta tgatttcaag
    20881 tatattgctg tagcgctgat cacattagga ttaacaattt tcttcaacat gtggctaaga
    20941 ggttttctcg gattgattcc gattttacta ggtatcatta gcggatacat cgtagccttg
    21001 ctgtttggaa tcgtggatac taagccaatc gtagacgctg cctggtttgc tgttcctaac
    21061 tttgagattc cctttgtaca atatgaacca aagctatatc ttggagcaat cacaacaatg
    21121 gcacccatcg cttttgtgac gatgacagag catatcggcc atcttatggt attgaataaa
    21181 ctaactaaac gtaatttctt tgaagaaccg ggtcttcata agacgttgac aggtgatggg
    21241 ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta cggtgaaaac
    21301 attggtgttc tggctatcac acgtgtccat agcgtgtttg tgatcggtgg agctgcagta
    21361 tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag tatccctgga
    21421 ccagtcatct caggaatcag ttttatctta tttggagtaa ttgccgcaag tggaatgaag
    21481 attttaatcg agaacaaaat cgattttgat aaaaagaaaa atctgttgat tgcttcagtt
    21541 atcttagtag taggaattgg cggactcgtc tttgaagtag gcacatttac attgtcggca
    21601 atggcattag caactgtact tggcatcatt ttaaatctta tcttaccaga aacttcacga
    21661 agcgaagaaa actaaggagg aaacattatg atcatcaaat cagaacgtat cagcttaaag
    21721 catttattga ccgttgaggc acttactgat caagaagtta tgggcttgat ccgcagaggg
    21781 caggaattca agcaaggtgc taattggact ccgcaaaagc gccagtactt tgctacaaac
    21841 ctctttttcg aaaatagtac tcgcacacat aaaagcttcg atgtagcgga gaaaaaacta
    21901 gggctagaag tgattgaatt tgaagcaagc acaagctctg tccaaaaagg ggaaacacta
    21961 tacgatacag tactgacgat gtcagcatta ggcgtagacg tagcggttat ccgtcatggt
    22021 gatgagaact attatgacga actgatacaa agtaaaacaa tccagtgttc gattatcaat
    22081 ggaggagacg gaagcggaca gcatccaacc caatgcttgc ttgatttgat gaccatttat
    22141 gaagaatttg gtcattttga aggtctgaaa gttgccatcg taggtgatat cactcactct
    22201 cgcgtagcta aatccaatat gcagatgtta aaacgtctag gcgcacaagt attcttctca
    22261 ggtcctagag aatggtacga cgatgaattc gaagtgtacg gacattatat gccattggat
    22321 gaactactag atcaagttga tgtcatgatg atgcttcggg tgcaacacga acgccatgat
    22381 ggaaaagaaa gtttctcaaa agaaggctac catcaggaac atgggttaac agttgagcgt
    22441 gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag caccagttaa ccgcgatgta
    22501 gaattagctg attcattagt ggaaggatta cagtcgcgga tcgttcagca aatgagcaat
    22561 ggagtattcg tcagaatggc aatcttagaa gcagtgctaa atggaaaagc gtaattatag
    22621 gaggatgaaa aaatgaaaac actcattaaa aacggtcaga tcaatacacg aaaaaatatg
    22681 acgacaccag cagaaatttg gatagaagat gggcgaatca aagcaatcgg tacaggattc
    22741 tcagaagccg aatttgatga agtgtttgat gcaaagggac aattgatcac acctggttta
    22801 gtggacgtcc atgttcattt gagagagcca ggatttacat acaaggagac gatcgaagct
    22861 gggactcgct cagctgctcg tggcggtttc actactgtat gcgcgatgcc gaatttaaat
    22921 cctgtacctg atacggcaga aaagctgaga caagtatatg acatcattag aaaagatgca
    22981 gttgtgaagg tattgcagta tgcgccaatc acagaaaatc tgcgaagtga gaaattagtt
    23041 gatcaagaag cactgattga agaaggtgct tttgctttca caaatgacgg tgtaggtgtc
    23101 caaacagctg ggacgatgta tctggctatg aaagaagctg caaaaaacaa taaagcccta
    23161 gtagcccata cagaagatga atctttatta tttggcggcg ttatgcacgc tggcaagaag
    23221 gcagaagaac ttggtcttcc cgggatctta agcgtaaccg agtcttctca aattgctaga
    23281 gatctgttgc tagctgaagc aactggtgtt cattaccatg tttgccatgt ttctactaaa
    23341 gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac agcagaagtt
    23401 tcaccccatc atcttatttt gatcgacgaa gatattcccg aagatttcgg cttttggaaa
    23461 atgaacccgc cgctaagagg acgggaggac agagaagctt tgatcgaagg cttgcttgac
    23521 ggtacgatcg attgcatcgc tacagaccat gctccccatg gattagaaga aaaaagccaa
    23581 agtttcatga aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca actgatctat
    23641 actcattttg tagaaactgg tcgattcacg ttggaacaag tgatcaattg gttggcagtt
    23701 aaaccggctg agatttttgg tttgaatgca ggaacgctaa cggttggtgc ccccgctgat
    23761 gtagcagttt ttgatattac acaaacttgc acgatcgata aagaagactt tttatcaaaa
    23821 ggggagaata caccttttat cggctggaaa gtaaaaggcg aaacacaaat gacttttgtc
    23881 aacggaaaac ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc ttatattaga
    23941 agatggaaca gttttcgaag gaaaggcttt tggtgcagat gctagtgtct tcggagaaat
    24001 cgtttttaca acaagtatga ctggttatca agaaactatc actgatcaaa gttttaacgg
    24061 tcaaatcatt acatttacct atcctatggt gggtaattat ggggtaaata gagatgatta
    24121 tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa gaacatgcac gtgtagcttc
    24181 aaactggcga aaccaaatga cgctagattc atttttgaaa caaaaaggaa ttcctggaat
    24241 atcaggtatc gatacgcgcg cccttactcg caaaatccgt gaagtcggaa cgatgaaagc
    24301 tagtatcgtt gatgctggag attccttcga acatgcattt gatcagctga aagcaaccgt
    24361 tctagcaacc aatcaagtgc agcaagtctc aactaacaaa ccttatccaa gtccaggaac
    24421 aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat agtattttac gagaattgag
    24481 taaaagaaac tgtaacctca cagtacttcc ctataacaca gatgccgaga cgattttagc
    24541 gctatcacca gacggagtca tgctgacgaa tggacctggt gatccaaaag atgtgccaga
    24601 agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg atttttggta tttgtctcgg
    24661 tcaccagtta tttgctttag ccaacggtgc agatacctat aaaatgaaat tcggacatcg
    24721 aggattaaat cacccggtac gagaagtagc gaccggaaga attgatttta cttctcaaaa
    24781 tcatggctat gcagtcgatg aagccacgat tgatccagaa aaactcatcg taactcatgt
    24841 agaagtaaat gacggaacag tagaaggagt caaacacaga gattatcccg ctttcagtgt
    24901 gcagtttcat ccagatgctg cccctggtcc gcatgatgca ctacatttat ttgatgaatt
    24961 tatggaaatg atggacgcag gaaaggagca gtaaacagat gccgaaacgt acagatatca
    25021 agaaaattat ggtgattgga tctggtccga tcatcattgg tcaagccgca gaatttgact
    25081 acgcaggaac acaagcctgc cttgccttaa aagaagaagg atatgaagtg gttttagtaa
    25141 attctaatcc tgcgacgatc atgacggata aagaagtggc agatcaagta tatatcgaac
    25201 cgatcacact tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat gctctccttc
    25261 caactttagg cggacagaca ggcctcaata tggcaatgga gctggcagca tcaggtattt
    25321 tagaagaatt gaacatagaa cttctaggaa caaagctttc tgcgatcgat caagcagaag
    25381 atcgtgatct attcaaacag ttgatgaaag aattgaatca gccaatcccc gagtctgaaa
    25441 tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa aatcggctat cctgtcatcg
    25501 ttcgtccagc ctttacttta ggcggaactg gtggaggtat gtgtgaaaat gaagaagaat
    25561 tacgtatcgt atcagaaaat ggattaagtc tttcccccgc tacccaatgt ttgattgaac
    25621 gaagtattgc aggttttaaa gaaattgaat atgaagtgat gagagattcg gctgataatg
    25681 caatcgttgt ctgcaatatg gaaaattttg acccggtggg tatccataca ggtgattcta
    25741 tcgtatttgc ccctagtcaa acattgtctg accatgaata tcaaatgctt cgtgatgctt
    25801 ctctttcgat tattcgagca cttaagatcg agggaggctg taacgtccaa ctcgctcttg
    25861 atccacatag ttttaattat tatgtgattg aagtcaatcc gcgtgtttct cgttcatctg
    25921 cgttagcaag taaggcgact ggctatccga tcgctaagct agctgctaaa atagcgcttg
    25981 gattaacatt agacgaaatg aaaaatcctg ttacaggaac gacttttgca gaatttgaac
    26041 ctgcattaga ttatgttgta gctaaaattc ctcgctggcc gtttgataag tttgaaaacg
    26101 gagaacggct tttaggcacg cagatgaaag caactggtga agtgatggct atcggtcgga
    26161 atatcgaaga gtctttattg aaagctgtgc gttctttaga aatcggtaca caccatatcg
    26221 aacttcctga gttggcagac atcacggacg atgacctaat ccaaaaagtg gttcgagcac
    26281 aagatgatcg cctgttttat gtagctgaag ctattagaag agggtacacc atagaagaat
    26341 tagcggattt gacaaaaatc gatttattct ttttagataa actgttgcat atcgtagaaa
    26401 tcgaacagca gttgaaacag cagccgttgg atgacaagat attgaaaaca gcgaaacaaa
    26461 acggttttac agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa gccgttcgcc
    26521 agatgagaaa agaaaatgat ttgattcctg tatataagat ggttgacact tgtgcagcgg
    26581 aattcgaatc aaaaacacct tatttttata gttcctatga atatgaaaat gaaagttcac
    26641 gatcagaaaa gccctcaatt ctagtactgg gatctggtcc tatccgtatc ggacaagggg
    26701 ttgaatttga ctatgcgact gtgcattctg tcaaagcaat ccagcaggca ggatacgaag
    26761 caatcatcat gaacagcaat cctgaaacag tttcgactga cttttctatt tcggataaat
    26821 tatattttga gccattgacc ttcgaagatg tgatgaatgt gatcgagcta gaacagccga
    26881 taggagtaat cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa cctttgactg
    26941 ctgctggtgt aaaaatctta gggacaacga tcgaagactt ggatcgtgcg gaaaatcgtg
    27001 atttattcga gcaagcgctg aaagagttgg atattcctca acctttaggg gatactgcta
    27061 caagtaaaga agaagccgtg gttatcgctt cgagaattgg ttatccagtt cttgttcgtc
    27121 caagctatgt attaggcgga cgtgcgatgg aaatcgttga aaaccagcga gatttagaag
    27181 attacatgga acatgctgtg aaagcatctc cagaacatcc agtactagtg gaccgttact
    27241 tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta ctcatcccag
    27301 ggatcatgga gcatattgag cgtgctggtg tccattcagg ggattcaatg gctgtgtatc
    27361 cacctcaaac attatctgaa tcgatcaaga aaacgatcga agattacacg atccgcttag
    27421 cattaggttt gaattgtgtc gggatgatga atatccagtt tgtcatccat gaaaatgagg
    27481 tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt tccattctta agtaaggtga
    27541 caggaattcc aatggcacaa attgcgacga aagctatttt gggagagaaa ctaaagacac
    27601 ttggttatga aaacggactt tatccagaaa caaaagaagt tcatgttaaa gcacctgtct
    27661 tttcttttac gaaattgcag aaagttgata cttacttagg gcctgaaatg aaatcgactg
    27721 gagaagtaat gggatctgac agacaattag acaaagcttt gtacaaggca ttcgaagctt
    27781 ctggattaca cttgcctgaa tatggagccg tattatttac gattgcggac gaaacaaaag
    27841 aagaagcact agaattagct aaacgtttta cagaaattgg ctatagtctg ataggaacga
    27901 aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta gcgaaaattt
    27961 ctgaggaagc agcagaaaaa aatgtagtcg acttaataag agatggtaaa acacaagtgg
    28021 ttgtcaatac gattgataaa gatcggggaa atgcttctaa agacggtttc atcatccgaa
    28081 gagaagcagt tgaacatggt attccattgt ttacttcttt agacacagca gatgcgatcg
    28141 ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat ttaacggcga gcacatgtta
    28201 agacaaaaag acgaaaaacc gttgcaggag gggacagatg aaacaagaaa tgatggaaat
    28261 cgttcagcaa aaacagctgg caccaaagat ttttgaaatg acattgaaag gtgatctagt
    28321 aaaagaaatg cagcaaccag gacagttctt gcatattctc gtgccgcgtg cagacttgct
    28381 attacgccga ccgattagct tgaatcatat cgaccacgaa acaaatactt gccgtatcat
    28441 ttatcgggtg gaaggtgagg ggactagagt cttctctact ctttctgccg gagaccattt
    28501 agatgtcacg ggtcctttag gcaatggttt tgatttgtcc aatcttcata caggagacga
    28561 agtatttata atcggtggag gaatcggtgt ccctcctcta tatgagttat caaaacagtt
    28621 agtcgaaaaa ggaattaagc caacgcattt tttaggtttt gccacacatg aagtcgttta
    28681 ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc gctactgacg atggaacata
    28741 cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg aagacggaac ctacagctgt
    28801 ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa cagctgtttt cttcgcatca
    28861 aaatgtccag ttatcattag aatcccgcat ggcatgtggg ataggggctt gctatgcttg
    28921 cgtttgtcat acgccggaag atgaaacagg aacaaaaagt gtgaaagttt gtgacgaagg
    28981 accgattttt aaaataggag aggtaattat atgaatgaga atcctttagc agtcaagctc
    29041 cccggtctag aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg ttttggcaaa
    29101 gaatacggaa aatactatga tttgaatcag cttggatcga tcatggtcaa ggcaacgact
    29161 ccaaatgccc gctttggcaa tcccacacca agagtggcgg aaacaccatc tggcatgttg
    29221 aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta tgcataaact tcttccagaa
    29281 ttagaggagt acaatgagtt acccatcatc gcaaatgttg ctggtgcttg tgaagaagac
    29341 tatgtagaag tttgtagcaa aattggcgaa gcgccaaatg tgaaagcaat cgagctgaat
    29401 atttcttgcc caaatgtcaa acatggcgga attgcttttg gaacagatcc tgatgttgct
    29461 tttcaattaa cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt caaattgtcc
    29521 ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg aagctggagg ggcagatggg
    29581 tttacgatga tcaatactct tcttggtatg cggattgacc ttaagacaag aaaaccgatt
    29641 ttagccaatc aaacaggtgg actctccgga ccagcaatca agcctgtcgc tattcgtttg
    29701 attcaccaag tagcaagtat atccagcctt ccaatcattg ggatgggcgg cgtgcagaca
    29761 gtggatgatg ttcttgagat gtttatggct ggagcaagtg ctgtagctat agggaccgcg
    29821 aatttcaccg atccgtatat ctgtcctaaa ctaattaaag agctccctgt gcgaatgagc
    29881 gaactaggca tcgaaacgct ggaaagatta atcaaagaag tgagagagga acgtgaacga
    29941 tgaatcaact agaccaacga ccaattattg cattagattt ttcaactagg caagaggtag
    30001 aagactttct tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc ggaatggaat
    30061 tgttctacca agaaggacct gaaatcgtac gttatctaaa agaggctggt cataatgtat
    30121 ttttagatct gaaacttcac gatattccga atacggtcga aaaagcaatg cggggattgg
    30181 caaagcttgg tgttgatctc acctgtgttc atgccgctgg cggtattcgt atgatggagg
    30241 cggcaatgcg aggattaaaa gtggggactc cagaaggtgg aaaacgtccg ctacttctag
    30301 ctatcactca gcttacttct acaagtgaag aagaaatgca tgccgatcaa ctaatcgaag
    30361 tttctttaga aaaaagtgtg atccattatg ctagctgtgc caaaaaagca ggattagatg
    30421 gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga aatggcagga gacgaatttg
    30481 tttgcttaac tccgggtata agaccagaag gaacagtcgc aggagatcag acaagagtag
    30541 tcacaccttc tcaggcaaaa gagatcggct caacttttat cgttataggt cgaccaatca
    30601 ctcagtcaac taatccttat aaagcatatc aaacgattca aacagaatgg agctaaccta
    30661 aaatgaatgt tgaacaaagt atcgcaaaag acttattaga aattgaagca gtatttttaa
    30721 atccaagtaa tccttttaca tgggcaagtg gaatcaaaag cccaatttac tgtgataacc
    30781 gaatcacgat gagctatcca aaagtccgaa aagagatcgc aaaaggatta gcaagcaaaa
    30841 tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac tgcaacagca ggaatccctc
    30901 atgctgcatg ggtagcggaa atactggatt tgccaatggt ctatattcga agtaaggcga
    30961 aagatcatgg gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag aagatggttg
    31021 tgatcgagga tttgatttct acaggcggaa gtgtcttgga agcagctgaa gcagcaaaaa
    31081 gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac gtatgagttg ccaaaaggaa
    31141 aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat tctgtattga
    31201 ttgaagcagc tttagaagat cgttatatcg atgagcaaga attaactctg ctaaaagaat
    31261 ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt gatgaagata gagaaatccg
    31321 atttgtgtct ccagaatcat caattggaat agcaaaaatc caaaaactga ttgcgagaag
    31381 catcttttca tcaggtatga aaagaaactt ctatcgtgat cagtttttga tttttttaaa
    31441 attcattttt ctaagactgg ttagcagaaa gaagagaata atctcttctc ttttttctat
    31501 tatatataga ttttctctat tttttggtaa gggagctgtt tcaaaaatgg taaagagcgg
    31561 aaatgttgcg aatatcagtt atttaatgat atgatattcg gaaaaagaaa tctcaaaggg
    31621 aggtaacttt tatgggattg aatgaattta ttttaggatt gaacaattta gagacgatca
    31681 caagggcacc tggatttttt aaatatacag agcatacagt tgcagcacat tcgtatcgtg
    31741 tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa ccgatcgatt
    31801 ggaaactgct ctatgaaaaa tcattgaatc atgactacac agagcgtttt attggtgata
    31861 tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa acagttgaag
    31921 aaaagatgac ggacgaattc atcaaccaag agattccagc tgagttccaa gaagtttatc
    31981 gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg agaactatta gccatttcag
    32041 ataaggttga ccttctttat gaatcttttg atgaaatcat caaaaataac ccggaaaaag
    32101 tttataagga gatgttcatt gaggcagtga ctacgattaa aagctttgaa catcggcctt
    32161 ctgttcagta tttctttaaa gagatatttc cggaactatt gaaccaggaa ttttatggaa
    32221 aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa taaaaatgat
    32281 atgagatgca gggggaagta tagttgaaaa agaatatgga tgtcgaatgg ggctattctg
    32341 gagacaaagg tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga ggtgcggaat
    32401 ttccattgca gtcgcctatt catttgacca agggggaaga aaccaaaata gaaggagaca
    32461 cgctttcctt tcattatgaa aagcaacggt ttactgacaa ggaattcaaa aacacgattc
    32521 atttagtacc ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc tattatttga
    32581 cagatattca tttccacttg ccttctgaac atatcatcaa cggaaaacag gaagatattg
    32641 aatttcattt tgttcatatg aacagcaaaa aagagaatct agttgtgggt gtcatgtacc
    32701 agctaatggt acaggaaggt tggctgtata atcaagaaaa tggggaaagt tggaacttag
    32761 aaaaacatga gcattgggta aatccggatg tgttctttcc ggagcaaaaa agccatttcc
    32821 attatattgg ctcattgaca actccgccta ccagcgggcc aatccaatgg tttgtaatgg
    32881 atcatgtagg aaaactgaac caagagtttc ttctcccctt cgatggtcag tatgctcgac
    32941 cgaacaaccg accaatccag cccttgaatg gaagagaaat ttattatttc gaggagtttg
    33001 agcaatgaat attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg gtagtttcag
    33061 agaagcggca aagaaaatgt ttgtctccca gccaagtctt tcagctggta tcaaagaatt
    33121 agaaaccgag ttagggatca cgctgtttac tcgaacgaat cgaggggcat atttaacaga
    33181 agaagggcaa gacttcttaa atcgtgccga gaaaatactg gtccagcttg atacattaga
    33241 aactcactat ctaacgagtg aaaagagaga caatttttcg attgctgccc agcattatga
    33301 tttcttaggt cctttgaccg cacagctact cgaggaattc aaagacgaga tcaaacaatt
    33361 ccggatcatg gagacgacaa cggcgagagt gatcgaagaa gtcaaagagc agcacagtga
    33421 gatcggtatt atttatatga atgagcataa tcaagctggt atcagaagat atttacaaca
    33481 aggagagcta agtggccgca gcattggaaa attcaaaacg catatttttt taaggcaagg
    33541 acatccgcta gctacacacg cagagatcac caaagaagaa ttgttatctt atcctcaagt
    33601 tcgttttaca caggatggaa ataatttccc gtatttttat gaagacctta tcgagatccc
    33661 tgatcaagaa acagtgattt atacgagtga tcgagggact ttgatgaata tcgttcttga
    33721 aactgatgct tacgcttcag gatctggtat tgtgattgga gggattcgcg agcatttgcg
    33781 gttaatccct ttggcagaag gcgaactaaa tgaattttat atcatttatt cggcgaaacg
    33841 cccgctcagt gaaatcgctc aaagatttat caaagagctt attcggttac tgaaccagca
    33901 gagctaagaa aagcagatga gaacgctgtt tgttgaacac cgttaatcaa aaaagagggg
    33961 ttgtgacaga attttgtcac aactcctctt tttgtagcaa acaatttata actaattgct
    34021 gttgtgggaa agtacgcaaa tatgattcta gaaacactca ttgattacct gttttgtttc
    34081 atagctgtaa ttttttcttc ttctggtgtc acgataatcg tcttttggtt ttcataaata
    34141 acataaccag gcttggcgcc atttggtttt cggatatgct ttacctgtac aagatcaacc
    34201 ggcacttggg cagagtatcg gtattttgaa aaataggcag ctaactcggc agcttctgta
    34261 attgtttcat ctgagggctt gtcactttta atgatgacgt gcgaaccggg tatatttttt
    34321 gcatgaagcc aatagtcggt ttttttagct gtcttcattg tcagctggtc attttgaaga
    34381 ttgtttttac caacaagaat gagcgttcca tcagaagaaa agtattgatc aggctgagat
    34441 ggtttcttac gtttttgttt ttttaagttc ttctttttca ggtatccttc tgaagtcaat
    34501 tcttctttga ttgcttcgat atccattggg ccggcaattt ccagttgaga taagacagat
    34561 tcaagatatt ggatttcatc tttggcttct tggatttgtt cgccaatcag cttaacagcg
    34621 ttcttcagct tttggtaacg atggaaatat ttctgtgcat tttgatttgg tgtcaatgcc
    34681 ggatctagtg ctattttgat tggccgatct tcttcataat aattaggtaa aacaacttct
    34741 tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta gttcgccatc tctacggtaa
    34801 ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt ttttcaattt atttttattt
    34861 cgttttagtt cattttcgat ttttcgaatc agttctccgc cttgttgttt tgcccgatct
    34921 ttctctgctt tttcgtgata atagctatct aataattgac tgagtgttgg ataagctgtt
    34981 acagcagatg cctgttcgga caatacctga taagcaatcg gtgtgaaaaa atctttattt
    35041 tccgtttcgt aaaaagtagg aataggctga tggctgatgg ctgagaaaaa ttcgtcccat
    35101 acaaccatct tttcattagg acgttccagt aatcttttgg tcagctcttg agcggtatcg
    35161 aaaccgattc cctgaaactg acgttggatt gcttttggct ctagttcagt ttgggaaagc
    35221 cgctgaaaga ttttttcttt ctcggagcta aatgggttca agacctcttg ttttggcgga
    35281 gcaacatatt ctacaccagg aagcaaagaa cgataagtgt tttgagagct tccaatgtgt
    35341 ttgatcgcat ccagtatttt tccagtttct cgattaacta agacgatcgt actatgacgt
    35401 cccattaatt caacgatcaa tatgatattt tgtagatcac ccaactcgtt tcgtttagta
    35461 aagtggaaat ggatcactcg atcattttcg atttgttcga tgtcttctaa aatagcgcca
    35521 tccaaatatt ttcgaagcat catcacgaag ttaggcggat tatcaggatt ctgataatcg
    35581 atttgggtaa tctgtacacg agcataactt gggtgcgcag ataataataa tggatggttt
    35641 ttgccgcgtg aacgaatcac cagcacgatt tcattttcat aaggctgatg gattttcgag
    35701 atccggccag atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt aaatacacca
    35761 tcaaaggaca tggttcattc ctcatttctt ttcataagta tattcattat aacgaaaaaa
    35821 agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc ttggactaat
    35881 tacataaaaa acagtgtttc tatcgttgaa atccaaattt ttaggtataa taaaaaatag
    35941 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
    36001 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
    36061 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
    36121 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
    36181 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
    36241 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
    36301 atcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
    36361 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
    36421 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
    36481 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
    36541 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
    36601 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
    36661 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
    36721 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
    36781 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
    36841 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
    36901 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
    36961 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
    37021 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
    37081 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cctcgacgaa
    37141 ccagacaaaa aaaacaacag tcgttggggt attgcaatac gtgagccatc cggcattaga
    37201 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
    37261 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
    37321 acagctagta caatctgatc cagatgtctt gattggaatc gcaacacctg ccgctcaatc
    37381 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
    37441 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
    37501 gccgcctgtt gcttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
    37561 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
    37621 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
    37681 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
    37741 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
    37801 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
    37861 atatcaatta gggcttcaaa gtggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
    37921 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
    37981 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
    38041 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
    38101 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
    38161 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
    38221 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
    38281 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
    38341 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
    38401 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
    38461 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
    38521 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
    38581 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
    38641 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
    38701 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
    38761 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
    38821 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
    38881 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
    38941 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
    39001 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
    39061 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
    39121 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
    39181 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
    39241 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
    39301 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
    39361 tatgtaaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
    39421 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
    39481 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
    39541 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
    39601 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
    39661 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
    39721 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
    39781 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
    39841 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
    39901 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
    39961 atcaaacgct aagtggcttt ttttattgaa gcaagaacaa aggaggaata agatgatttc
    40021 tggacaaacc aaattagctg ggctctttgc tgatccagct tctcacagtt tatctccatt
    40081 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttcaaagt
    40141 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
    40201 aaacctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
    40261 agcgcaattg atcggagcag tgaatacaat cgtgcacgaa aagggtcgtt tgattggcta
    40321 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
    40381 gaaaaaaatg actctactgg gcgctggagg tgcagcaaag gcagtcactg ttcaagcagc
    40441 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
    40501 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
    40561 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcgac
    40621 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
    40681 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
    40741 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
    40801 aatcgcgttt gaattattga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
    40861 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
    40921 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
    40981 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
    41041 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
    41101 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
    41161 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
    41221 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
    41281 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
    41341 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
    41401 atatgtagct gaacatgaca caagcttaga ggaagcgact tcagaaacct ttcatgggca
    41461 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
    41521 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
    41581 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
    41641 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
    41701 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
    41761 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
    41821 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt atgcacgttt
    41881 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
    41941 tattttcagc caattgaaga atccgttgaa tctgatccgt gcaccttatg cagctggtaa
    42001 agctttaggg tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
    42061 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
    42121 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
    42181 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
    42241 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
    42301 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
    42361 gaagccatcg ctttttatga aaaagttttt gagactgaag catcaggtat caccacatac
    42421 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
    42481 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
    42541 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
    42601 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
    42661 gctgttccgt gggctcaatt gtatgggatg gtaactgatc gatttggcat caactggcag
    42721 atcaaccaag agtgacagct aaagatgaat attttttgga aacatcatgc agcaaacgga
    42781 acatcgattg tttgcttgat gttttttgtg cctatttcga ctatacggta tctttttttt
    42841 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaatca agtaacggag
    42901 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
    42961 aaaccttcca acagcgaaag cgacatgaag ggtattatga aatcagcgat ccattgtttg
    43021 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
    43081 cttatgaaga gcgttgggca aaatggtcgt tagaaaattt gcctattttc ccagaaacct
    43141 tccgttacga agttccaagg gacaaacgta cacaattttc gatcgtaaaa cgagagttac
    43201 agtcagctga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
    43261 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttgtgga
    43321 tcaattctct tgaaaaagaa gcaatctatg atggttttaa gaatcttcaa cctgggaaga
    43381 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
    43441 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
    43501 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
    43561 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
    43621 agttttctgg aaaagtcgta caaaaaaaag catttaaaac ccaagaagag ttaacgaagg
    43681 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
    43741 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
    43801 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
    43861 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
    43921 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
    43981 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
    44041 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
    44101 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
    44161 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
    44221 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
    44281 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagcatgg
    44341 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
    44401 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
    44461 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
    44521 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
    44581 ccaatgaacc attactgacg agcgcagaaa tgactgcgca ctgggaaggg tacttgagaa
    44641 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
    44701 tacatttatt agcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
    44761 gcgggacacg acagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
    44821 aatgtaaaaa ggggatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
    44881 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
    44941 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
    45001 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccttgga
    45061 tcgaaaacag tggaacaaaa gcagtagatg gaaacaaaga aacatcaaga aaaaataatg
    45121 aaaacgaagt agagtaaatg aaaacaatga aatagcaaaa tatttattcc gaaaagtcag
    45181 aatataaata ccaataaaac taaaaaatat cagtcaatca agcaaaaatg ttggggtagg
    45241 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
    45301 ttatcttatg ggagcaatta cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
    45361 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
    45421 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
    45481 catgctaaag cgaaagaaac agcgaaaaca attgttgatg gtggtagcgg ctatgatcat
    45541 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
    45601 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa
    45661 gaaaaataaa agtatataca ttgattaaat gtatatactt ggatataatg tacttactat
    45721 agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag
    45781 aaaatctggc aatagcgatg tgacaactat cccaccagaa gtaaaagaat ttataggtgt
    45841 gcagacaggt gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa
    45901 agcacaagaa gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga
    45961 cgcgttaaaa gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc
    46021 aacgcgaaag ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    46081 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatccc
    46141 gaagtctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    46201 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    46261 ccagttgctt tttctcgtcg agaagctgtt gattttatca taatgattac cactagttca
    46321 ttggatttcg attcactaaa gtccaagata accaattatt tacgacaaac cgcaataaaa
    46381 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    46441 tttttttatg gttaatcata caaaatagtt ttttcataaa aatatggaaa atatggatta
    46501 attttgaaat aattgattta aaaggaatac aaa
//
LOCUS       DANKHK010000011        43123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000011 DANKHK010000000
VERSION     DANKHK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            39..1062
                     /locus_tag="PW109_000563"
                     /pseudo
     CDS             39..1062
                     /locus_tag="PW109_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1405..1661
                     /locus_tag="PW109_000564"
                     /pseudo
     CDS             1405..1661
                     /locus_tag="PW109_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2048..2275
                     /locus_tag="PW109_000565"
     CDS             2048..2275
                     /locus_tag="PW109_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2510441.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2291..3457
                     /locus_tag="PW109_000566"
     CDS             2291..3457
                     /locus_tag="PW109_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2510442.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3692..4735
                     /gene="hrcA"
                     /locus_tag="PW109_000567"
     CDS             3692..4735
                     /gene="hrcA"
                     /locus_tag="PW109_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2510443.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4829..5392
                     /gene="grpE"
                     /locus_tag="PW109_000568"
     CDS             4829..5392
                     /gene="grpE"
                     /locus_tag="PW109_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2510444.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5443..7272
                     /gene="dnaK"
                     /locus_tag="PW109_000569"
     CDS             5443..7272
                     /gene="dnaK"
                     /locus_tag="PW109_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2510445.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7423..8589
                     /gene="dnaJ"
                     /locus_tag="PW109_000570"
     CDS             7423..8589
                     /gene="dnaJ"
                     /locus_tag="PW109_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2510446.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8957..9694)
                     /locus_tag="PW109_000571"
     CDS             complement(8957..9694)
                     /locus_tag="PW109_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2510447.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9696..11198)
                     /locus_tag="PW109_000572"
     CDS             complement(9696..11198)
                     /locus_tag="PW109_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2510448.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11457..13451
                     /gene="uvrB"
                     /locus_tag="PW109_000573"
     CDS             11457..13451
                     /gene="uvrB"
                     /locus_tag="PW109_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2510449.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13461..16277
                     /gene="uvrA"
                     /locus_tag="PW109_000574"
     CDS             13461..16277
                     /gene="uvrA"
                     /locus_tag="PW109_000574"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2510450.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16540..17805
                     /locus_tag="PW109_000575"
     CDS             16540..17805
                     /locus_tag="PW109_000575"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2510451.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17809..18540
                     /locus_tag="PW109_000576"
     CDS             17809..18540
                     /locus_tag="PW109_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2510452.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18675..19559
                     /gene="rapZ"
                     /locus_tag="PW109_000577"
     CDS             18675..19559
                     /gene="rapZ"
                     /locus_tag="PW109_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2510453.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19556..20554
                     /locus_tag="PW109_000578"
     CDS             19556..20554
                     /locus_tag="PW109_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2510454.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20588..21523
                     /gene="whiA"
                     /locus_tag="PW109_000579"
     CDS             20588..21523
                     /gene="whiA"
                     /locus_tag="PW109_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2510455.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22091..22747
                     /locus_tag="PW109_000580"
     CDS             22091..22747
                     /locus_tag="PW109_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510456.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22926..23491
                     /locus_tag="PW109_000581"
                     /pseudo
     CDS             22926..23491
                     /locus_tag="PW109_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24190..24711)
                     /locus_tag="PW109_000582"
     CDS             complement(24190..24711)
                     /locus_tag="PW109_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2510457.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24909..28589
                     /gene="nifJ"
                     /locus_tag="PW109_000583"
     CDS             24909..28589
                     /gene="nifJ"
                     /locus_tag="PW109_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2510458.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELQKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHAPAYLHSYNLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVIPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28778..29665
                     /locus_tag="PW109_000584"
     CDS             28778..29665
                     /locus_tag="PW109_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2510459.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29662..30831
                     /locus_tag="PW109_000585"
     CDS             29662..30831
                     /locus_tag="PW109_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510460.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30855..32210
                     /locus_tag="PW109_000586"
     CDS             30855..32210
                     /locus_tag="PW109_000586"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2510461.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32282..34087)
                     /gene="glmS"
                     /locus_tag="PW109_000587"
     CDS             complement(32282..34087)
                     /gene="glmS"
                     /locus_tag="PW109_000587"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2510462.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34497..34787
                     /locus_tag="PW109_000588"
     CDS             34497..34787
                     /locus_tag="PW109_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510463.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34800..35825
                     /gene="sppA"
                     /locus_tag="PW109_000589"
     CDS             34800..35825
                     /gene="sppA"
                     /locus_tag="PW109_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2510464.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35828..36487
                     /locus_tag="PW109_000590"
     CDS             35828..36487
                     /locus_tag="PW109_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2510465.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36644..37576
                     /locus_tag="PW109_000591"
     CDS             36644..37576
                     /locus_tag="PW109_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510466.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37730..39019
                     /locus_tag="PW109_000592"
     CDS             37730..39019
                     /locus_tag="PW109_000592"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2510467.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39266..40516
                     /gene="clpX"
                     /locus_tag="PW109_000593"
     CDS             39266..40516
                     /gene="clpX"
                     /locus_tag="PW109_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2510468.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40599..41186
                     /locus_tag="PW109_000594"
     CDS             40599..41186
                     /locus_tag="PW109_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2510469.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(41260..41415)
                     /locus_tag="PW109_000595"
     CDS             complement(41260..41415)
                     /locus_tag="PW109_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510470.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            41551..42879
                     /locus_tag="PW109_000596"
     CDS             41551..42879
                     /locus_tag="PW109_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510471.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42890..43099
                     /locus_tag="PW109_000597"
     CDS             42890..43099
                     /locus_tag="PW109_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510472.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        14295 a         7365 c         9312 g        12151 t
ORIGIN      
        1 aatgcaatta tattgatatc attaggagct gcattgttat gaatccaaca ttgattacta
       61 agaaagaatt actaaaaaaa ttagatattt ctactggtgt attagctaat ttaattagaa
      121 atggcatgcc aaaagaggga gaaatgttta atcttgataa gattattaca tggcgagaaa
      181 attggtctaa aaacatatta ggtgagttag aagttggtag agtatatact aacaaagaaa
      241 tttcagagaa gtttaaatgt tccaaacagg gtggaatgag aagatctcat caaactaata
      301 ctcttgtact gtttagtgat caaacaggaa gtaatgttta taaagataag tggttaaatg
      361 gtattttaca atataccggt atggggctaa aaggggatca agtattggat aagaatcaaa
      421 ataaagtttt agccaattca aaaagtaatt ttgtaaagat tcatttattt gaaacattta
      481 aaccaaaaga acacacatat ctgggagaag tatatttagc aggacaaata tatactgtta
      541 atgaaaaaga tagttcgggc aacagcagaa aagtatataa atttccatta gctttaataa
      601 atcaagagca attgattgaa gataaagata tataaccagg aagaaaatca aactagacat
      661 attcgtaatt tatctaatgc taaattagag gaagaagcaa gaaaagttag taattataat
      721 aaaaaaatgg ctgcaaaata tgttaacgaa gaggagtttt gcttctcaaa aacaaaaata
      781 tataaacgta atcccttcgt atctgaatat gttaaacgac ttgcaaaagg tatttgtcaa
      841 ctttgccaag aaaaagggcc atttataaaa gacggtgtac cttatttaca ttgtcatcat
      901 atagaatatt tgtcgcaagg tggaaaagat gtgattgaaa actgtatcgc gttatgtccc
      961 aattgtcatg ctagaattca tgaacttgag ttacctgacg ataaagaaaa attgctgaag
     1021 atcgttgaaa aaagagaaaa tagcttttct aaaataccgt aatttatttt tataggacaa
     1081 ataagctaat gtgagctatg ccttctatat aagactaagt tctattataa gtaacgaagt
     1141 aattctcgtt ccttcctgta gtaatatttt ttaaataacc tataggcttc ttcaaaagta
     1201 catcctccag taaattctaa gtactggagg attctttttc tagtgttgct ttactcatga
     1261 aaaagtagtt ggctacttca tcaaaatttc tgaacttttt taatgtataa taaagagctc
     1321 gatcatttac tacaaggatg ctagctccta cattttcaca gttttttagc aagtaaattt
     1381 ttctaaaatc acatcaggaa atgcatgaga ttctttaaga gaggcaatta tagggctatg
     1441 attacggttc gtgtaagtga cgccaaaaaa gaatggcttt cttatatggc tgaattttat
     1501 gggattactt tatctgaact tctaaaaaac tattcgatgg aacaactaga agatgaatac
     1561 aatcgtcaaa cggctgaaat tgcccataaa cattggataa gaagtgggaa aacaacagta
     1621 ttcatggaag aatccttaat gaatttggtg ggctggaatg acctgccgtt taaaattcac
     1681 gcccgaaatt cagaagcagc tctgattggc aaatacgtgt attggtagag atagaagata
     1741 atcaattcgt tgttatcgct attcaagtag ggcaccgaag aaatgtctat gattaagaga
     1801 ctgtttattt ttacaaaaaa ataataattg gaatctagtt aaaactcttg aagtgagaga
     1861 tttgactaga tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt
     1921 gtaaaaaaat catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata
     1981 cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga
     2041 ggaatctgtg aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag
     2101 ctttgcgatt accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa
     2161 gcaagcattt ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt
     2221 ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga
     2281 aagaatttca atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca
     2341 tatcccattt tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg
     2401 gcagccagta gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag
     2461 tcatcctaca gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc
     2521 tgctaaacaa cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg
     2581 taatgagttt actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat
     2641 gaaaaactat ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt
     2701 gaaaaaaatt ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga
     2761 agcagaaaat tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact
     2821 agaaggatat cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt
     2881 gtactcattg atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact
     2941 cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga
     3001 aaagcatgga cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca
     3061 acataacctc atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg
     3121 ttatcttgga gatattcgtt accgcaacaa aggaccaatc caacactatt tacgcccatt
     3181 acgagcaaat gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga
     3241 agaagaaatg ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga
     3301 tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga
     3361 agggttactt gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg
     3421 gaacaatgtg tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat
     3481 cgaaacttga aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata
     3541 actggattta catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg
     3601 agtctttctc ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag
     3661 aatatatgag tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt
     3721 acgtctgatc atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat
     3781 gtctgaggga gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga
     3841 tgaaggattg ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta
     3901 tcgttattac gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct
     3961 gattcgtcag tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc
     4021 tgagatatta tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga
     4081 ccgccgactg actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt
     4141 cgtaacagat atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag
     4201 tcaggaacta gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat
     4261 gacggtgtac cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac
     4321 gacagaaggg atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt
     4381 ttatgtcagt ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt
     4441 caaatcgatg ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat
     4501 ggatagcaat attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat
     4561 gagtatgatt caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt
     4621 agggcctact agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga
     4681 actagcagct aaacttgctg attactaccg ctcgcttgac ttgtcaaact cgtaatgtga
     4741 ataacgaagc tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct
     4801 gactgaataa tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga
     4861 aatgacagat gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat
     4921 ttcagaagct gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat
     4981 ggaagataaa tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa
     5041 tgagcgtgag caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc
     5101 aattgacaac ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt
     5161 gaaaaaaggc gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat
     5221 tgaaaaaatc cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac
     5281 tgttccagcg actgaagata caccagccga tacgattgta gaagtactac aagaaggcta
     5341 caaattacat gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg
     5401 atataaaact tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt
     5461 attgacttag gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc
     5521 atcgctaacc cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa
     5581 atccaagtag gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca
     5641 atcaaacgcc atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca
     5701 ccacaagaaa tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta
     5761 ggtgaaaaag tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt
     5821 caagcaacaa aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa
     5881 ccaactgcag ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta
     5941 gtattcgacc ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc
     6001 tttgatgtat tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa
     6061 atcatcgact acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac
     6121 aaaatggctt tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc
     6181 gtatcaagca cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat
     6241 ttggaaatga cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt
     6301 acaaaagtac cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac
     6361 gaagttattt tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa
     6421 gaaacaggaa aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct
     6481 gctatccaag gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca
     6541 ccattgtcat taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat
     6601 acaacgattc caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca
     6661 gtagacatcc atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga
     6721 agattccaat tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca
     6781 ttcgatattg ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa
     6841 gaacaaaaaa tcacgatcaa atcttcttca ggtctaacag acgaagaaat cgaacgtatg
     6901 gtgaaagatg cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta
     6961 cgtaatgatg tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc
     7021 aaagtggacg aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct
     7081 gttgaagcaa acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt
     7141 caaaacttga ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca
     7201 gaagcagctc aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc
     7261 gacgataaat aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt
     7321 ttcttttgaa aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa
     7381 caagcgacgt gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat
     7441 tattatgaag tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat
     7501 cgtaagcttt cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa
     7561 ttcaaagaag tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac
     7621 gaccaatacg ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga
     7681 tttggtggat tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac
     7741 tctttctttg gaggaggcgg tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat
     7801 ttgcaatata cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc
     7861 aagtataatc gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca
     7921 caaccaacta cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg
     7981 ccacttggac gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa
     8041 atcaaagaac cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt
     8101 aaagtgaatg taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga
     8161 gaagctggag aaaacggcgg accatttggt gatttgtatg tggtttttcg tgtagaagaa
     8221 agtgatatct ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta
     8281 caagcagctt tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag
     8341 atccctgctg gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag
     8401 ttacgtggtg gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat
     8461 ctaaatgaac aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt
     8521 gaagaacagc aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag
     8581 cggaagtagt ttttctagta aaaatataaa gtagacttgc ccaaattttc tatttctagt
     8641 aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatacgtt cgcctggttt
     8701 ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca
     8761 tagtttctga aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt
     8821 cttatgcgat ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc
     8881 tggtacagtt aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg
     8941 gcatttttgg tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat
     9001 tttgcggatt ttcaaagatt tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa
     9061 tgaacatgac acgattggcc acttctttag cgaagcccat ttcgtgcgta acgataacca
     9121 tcgtcatacc ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcgg
     9181 ggtcgagtgc agatgtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac
     9241 gagcaatagc cacacgctgc ttttgtccac cggaaagcat ttcaggatac atatcttttt
     9301 tatcttctag accaactgtc tgcagcaatt tgattgcttt tttctctgct tcttctctag
     9361 attctttctt caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga
     9421 ataggttaaa gtgctggaat accattccga tatgttgtct caccaagttg atatctgtgc
     9481 ttttatccat caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac
     9541 gattcaagca acgcaaaaat gtactttttc ctgaacctga agggccaatg atacagacga
     9601 catctccttc gttgatggac acattgatgt cgtttaatac tgtgttatcg ccgtattttt
     9661 tgactaaatg ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact
     9721 tttgctaatt ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa
     9781 acatacgtac tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact
     9841 acaccgattg cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat
     9901 gatgggatca tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa
     9961 gaaagaccta aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct
    10021 cgaacaatct ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg
    10081 aagtcaggaa tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga
    10141 accatcattg gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg
    10201 cgtattggtg ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag
    10261 gagatcaaag ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt
    10321 tggataagtc caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa
    10381 ccatccgcta catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc
    10441 aatccttcat tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca
    10501 tattttccgc cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca
    10561 taaccgatta ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg
    10621 tacaattgat cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg
    10681 cttaaaaatt cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct
    10741 tcataagact tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag
    10801 taagaatcag aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc
    10861 attccgtctg cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc
    10921 attcggatgt taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct
    10981 tcgtactcgc cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt
    11041 acgtatacgt cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc
    11101 gttgtagtgt tgtcagcttg tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt
    11161 aagaaaacaa atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa
    11221 tattttatta ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg
    11281 aaaaaaatgc gagtttctaa cattttatgt tataaaacgt aacatgcata ataagataaa
    11341 aaagtggcga aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc
    11401 cttttctttt tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga
    11461 ttgagagaga tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc
    11521 agcctgaagc gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct
    11581 tattaggtgc gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga
    11641 ataaaccaac actaattatt gcccataaca aaacattggc tggtcagctg tacggggaat
    11701 tcaaggaatt tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc
    11761 agcctgaagc ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg
    11821 atgagatcga caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga
    11881 tcgtcattgc atccgtttca tgtatttttg gattaggttc gccttttgag taccaaaaac
    11941 aagtagtttc gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag
    12001 tcagtatcca attcgaaaga aatgatatag attttcagcg cggtcgtttt cgagtgcgcg
    12061 gtgatgtcgt ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt
    12121 ttggagatga ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag
    12181 aaacggaaca cgtggcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg
    12241 agcatgccgt agcgaatatc aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg
    12301 aaaacaaact tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga
    12361 tgttagaaat gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta
    12421 aagaaggtga accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg
    12481 cggacgaatc gcacgtaacc atgcctcaag tcagaggaat gtataatggt gaccgagcga
    12541 gaaaacagat gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac
    12601 ttcggttaga agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag
    12661 gaccatatga acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc
    12721 tgcttgaccc agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg
    12781 aaataaatga acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa
    12841 tggcagaaga cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata
    12901 gtgatatcaa aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg
    12961 atgtgttagt tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg
    13021 ttgctatctt ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa
    13081 cgattggacg agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa
    13141 cagattctat gcgtctagcg atggatgaaa catcaagacg ccgtacgatt cagcagaagt
    13201 acaatgaaga acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt
    13261 ctattacgaa agaatcggaa gatgatacaa aagaagcggt acaagtctcg tatgaagaaa
    13321 tgacgaaaga agaaaaagat acgttattga tgaaattaga aaaagaaatg aaaaatgcgg
    13381 cgaaagcatt ggattttgaa acagccgcta atgtgcgaga tatgatttta gaattaaaag
    13441 cttcaaagta ggaggacaac atggctaacg ataaaattac gattcacggt gcacgtgccc
    13501 ataacttgaa aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt
    13561 tgtccggttc tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga
    13621 gatacgtaga gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg
    13681 acgtagatag catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta
    13741 aaaatcctcg gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt
    13801 ttgcacgtgt aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag
    13861 tagatcaaat ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg
    13921 caccgatcgt ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag
    13981 agggatacgt ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc
    14041 tggagaaaaa caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag
    14101 ggattcgttc tcgtctattt gattctttag aggcggctct gcgattggca gacggctatg
    14161 ctctggttga cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt
    14221 attgcggatt tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg
    14281 gagcttgtcc agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga
    14341 ttccagatag aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct
    14401 ctcagtatta tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata
    14461 caccattcga agaattaccg gaagagcatc aggatatcat cttgaatggg acagatgatc
    14521 ttttccattt ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg
    14581 aaggggtatt gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg
    14641 agcaaatgcg tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga
    14701 atccccaagc tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt
    14761 ctattcaaaa agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa
    14821 ttgccaaacc gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac
    14881 tagactatct aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa
    14941 tcagattggc tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac
    15001 catcgatcgg attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc
    15061 gtgatcttgg caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag
    15121 attatctgat tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag
    15181 gcactcctga agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga
    15241 aaaaatcaat ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca
    15301 ctggtgcttc agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg
    15361 tagcagtcac aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga
    15421 aatcgttagc gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt
    15481 ccggctacga aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc
    15541 cgagaagtaa tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc
    15601 aaacgaacga ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag
    15661 gcggtcgttg tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc
    15721 ctgatgtcta tgttccttgt gaagtatgtc atggcaaacg atataactct gaaacattag
    15781 aagtgcatta caaaggaaaa agcattgctg atattttgga aatgacagta gaagatgctg
    15841 tagaattctt caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg
    15901 gcttaggtta tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac
    15961 ggatgaaact tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag
    16021 atgaaccaac gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa
    16081 gattagtaga tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa
    16141 cagcagatta tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg
    16201 cgactggaac accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga
    16261 aaaaaataat ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg
    16321 aatttttgct tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta
    16381 cactgattgg tatagacaaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc
    16441 aatcggagga gtttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt
    16501 ggaaaagaaa aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat
    16561 gaagaattta tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct
    16621 tttaatgaag catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc
    16681 cgctacagta aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtgttt
    16741 atcgagacga tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt
    16801 ttgattgctt gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca
    16861 gaatatttta tctgtccata tattgattat tctttatttg aaagactgat caataaagta
    16921 agcttttatg aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga
    16981 gaagaaatgt tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct
    17041 ttgaaacctg caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct
    17101 tttattttag agactagaga agagttcgat ttaattttag gccggatata cgaagccggt
    17161 tataaaagtg aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg
    17221 ctaaatgcgt atgtagatga agatcatcaa gttcggatga tgtgtcttgg tcatccactg
    17281 ttagaagatc ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac
    17341 gaaaaaatct atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc
    17401 aatttcgata tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg
    17461 agacaaggtc gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc
    17521 acagaagatc gagtgtttaa caaacccttt gttgaaacga cctatggaac gaatcaatca
    17581 gataaagcaa gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa
    17641 aatgaagaca aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgtg
    17701 ttttatgaaa aagaccggtc aatcaagcgc tggttgttga tgaaatatat gttccataac
    17761 tatattccaa gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt
    17821 ttcagtattt taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac
    17881 caccgaacga aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca
    17941 acagttcctg atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt
    18001 ctgctggatg atattgagaa acaaaattta ttgcagccga acttcattgt gttgacttgt
    18061 aacaccgcac actacttttt tgaagaatta caagcggcaa cggatattcc tatccttcat
    18121 atgccaagag aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt
    18181 ctaggaacag aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttgga
    18241 ttcgaaacag tgattcctga tactgttctt caagaaaaaa tcaattatct gatctatcat
    18301 gagatcaagg aatcggatta tttgaatcaa gagctatatt acgaaatctt agaagaagcg
    18361 gtagagcgcc taaactgtga aaaagttatc ttaggttgca cagaattgtc attgatgcat
    18421 gaatttgctg aagataacca ttatccagtg atcgatgccc aatctatttt agcagatcgg
    18481 acgattgaac gagcgctagc tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa
    18541 aaaaattgga tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag
    18601 aaaaagaaaa ctggtaatga tacgcccgga atgatataat tttactatgc acaatgtgag
    18661 aggagttcgt aattatgaca gataatttgc aattagtcat cataacaggt atgagcggtg
    18721 cagggaaaac agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata
    18781 tgccgcctcg attgattcca aaattctggg aattaatcaa agaatcaggc aaagtcacaa
    18841 aaatggcttt aggagtggat ttacgttctc gttccttctt tgaagaaatc caaaacatgc
    18901 tgatcgaaat cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag
    18961 acgaagaact agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg
    19021 gtttgatcac tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag
    19081 cttccgtgat cattgatacg acgacattga caccacgaca attacgtgaa aaaatcaatg
    19141 agtcattcaa atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta
    19201 aatacgggtt gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc
    19261 attatatccc ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga
    19321 tgtcctttga tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat
    19381 tgcctggata cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg
    19441 gtcaacaccg ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata
    19501 aagtcaatgt gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa
    19561 aatgaaaacc taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct
    19621 gccagttatc ctgaaaagtt tgcgaaatca aggagcagat atcacagcag tagtgactgt
    19681 tgcagatgat gggggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg
    19741 tgacttgaga aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt
    19801 ccaataccgt ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat
    19861 cattgccgca gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa
    19921 aatgatgcat gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc
    19981 tgtctttaaa gacggaacga ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac
    20041 cattgaccgt gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa
    20101 agtcgtttca gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac
    20161 cagtatcctt ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc
    20221 tgaaaccgtc tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga
    20281 tgccgatcat atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt
    20341 ggtcaatacg gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct
    20401 agtacaagtc gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac
    20461 cgactttttg gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga
    20521 attgttccgt ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga
    20581 gaataggatg tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg
    20641 agaacatgcg aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt
    20701 gaaccagcag tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta
    20761 ttctcttttg aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa
    20821 attaaaaaag aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat
    20881 ggatcttgat atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg
    20941 aaattcgcaa aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa
    21001 taatccggaa acgagccgtt atcatttgga aatattttct atttatgaac agcataacca
    21061 agacatttgc gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa
    21121 tggctatatt tcttatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg
    21181 tgcaaccaac tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc
    21241 ggtcaatcgt ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc
    21301 taaacaaatc gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga
    21361 aaaacttcag gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt
    21421 aggggaaatg attccctcag gtgcgatatc aaaatcggga attaatcacc gaattcgcaa
    21481 aataaatgaa tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga
    21541 tacatgaagc aggagatgcg aatgtatcag ttttttttaa aagttatact tgcttttctt
    21601 ccgtcaaact ataattccat ctttactgga gtaacataat ttggaaaggc ttctattgtt
    21661 tttaatacaa tcgtataaaa agcggggaga caactgaatg aagattttag ttttgtacat
    21721 aaatttttgc ttaatataga agtcctgata tagataagat caagtattta tctataactg
    21781 gcctgattaa ttaaaaaact ggaaggaact gctcgatcat ccttgagaac tgctcttctt
    21841 acttctggtc aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta
    21901 gctagtcgaa aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat
    21961 ttattcagtg ataatcataa gttcataaat agtaattgag atgtatggtt ggaatacgtt
    22021 atacctgaac ttgaactacc aagatttggt ttagaatttg agaatcgatg tttataaagg
    22081 gagggcaaaa atgcgttttt ttacaacttt tactatcatt atgattgcag tactttttat
    22141 ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc
    22201 aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat
    22261 ttggagaact ccagcaccac cattgccaga gattacatat ggagaatttc cttttagact
    22321 tgagtatgaa ttgaatgaag aattacatgt gatagaagac acgttaatag ttgaatttga
    22381 tggatttgga atgaacgaag gcatagggag atatcgtaga tggacaagtc gtttagccag
    22441 cggagaagat ttagtgttgc tgttagaagt tagtgataat aagcaaatat tttattttcc
    22501 aggacctgct aattattaca tgggagatag gttgaatggt tataatcata cttttcctag
    22561 tgcttctttc attgaaaggg aaagaggcat tactagaagg gatattttac atgacaaaga
    22621 attattagaa caatttggtc cgcttgatca aaatacaata aatgaagaag aattgttaaa
    22681 tcaatataat ataaggctag tgaattggga aatatctgaa ccgattgtta acaattttgg
    22741 tgattagaaa acaattacga taaatagaaa caagccaaat catcagcaag taaaagctga
    22801 tgatttggcc tttcttatac gagtagtagg aattcatagc gaatattatt acgatcagta
    22861 gatattttga tattaaagga gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc
    22921 gaataatgag aaatttatca aatagaaaca aaatattaat cattattgtt gttatagctg
    22981 tgtttcatct gggaacgaat gcggttttaa gtagaataat attgggtcct aagccaccaa
    23041 gacctgcgat tacccgaggg gagtttgatt ttagattaga atatgaagta gatggggaaa
    23101 ggattgtgat agaagataca attgttgcat tatttgatgg attttctgca gatgcaggta
    23161 gcatggcatg gtatcgcacg tggcgtttgc atttggcgag tgacaagagg gcacaacaat
    23221 tattgttaca tgagttagat gatggtcgaa aaatatatta tcattttgga ggagctggat
    23281 tttatatggg gaagttagta gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg
    23341 cattcgaaac tccatatttt atatcgagcg tgggagagat acatctattg ggaacaggaa
    23401 agggttaact cttgaagagt tatataatga ctttggaatc cgtcttgtta gttgggaaca
    23461 caatccacca atagaaaatc gctttgaata atcgaaatct attaatccat ttcatcagcc
    23521 agtgaatgtt gtaatggaac aatttggttt agtgttcaaa accattgaaa tactaggctg
    23581 tgatcagcaa aaatgtatct gaataaaaaa gagagcaact attaaatgag aggagttagg
    23641 agttgttagc tttatacgaa tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc
    23701 catcttgcgt ttggtagacg agataggtat aagctaaaga aacaacttgt ttaaaataaa
    23761 cgacctagct caacaaacga ccatctaatc aactaacagc atacataaaa gcaactattt
    23821 ttgaagtaga attgaaatcg ggataactta aaaagtgatt gggatactag tgtgaatcac
    23881 cgtacataaa tattactcgt taagggaatt tgagaaattt tgtatgaaaa tttaagaatt
    23941 agttagtatg cgcaagtcaa aaatatgaat aaattatttg gtggagataa tttgctcagc
    24001 ttgtcataag aaatgagata atctggcaga tgcaagcttt tggacaccat ttattcggaa
    24061 taaggaggct gggacagaag cgtttaactc caagaaataa gaaggagttg cttctgcttc
    24121 cgccgtttat aggtgtttag agcgtgagac aaaagtgttc tttacttttg tcctacgctc
    24181 cttatttttt tattttgttt ggatcaattc ttttaattcc tctattgaaa tagattcatc
    24241 aaagatttct ttacccaaga tgacagtcgg aacaaatcga atatgtgctt gctcagcttc
    24301 gttgatgaca gcttggctca gctgttcgtc tttttgttct gtcagcttta gtttatctac
    24361 tgcaaaatcc gctacttctt ggagagaaag atgtttccac tcgtcttgag tatcgaatat
    24421 ttgtttgatc tctttgatcg ctttttgacc atcgctgatt gtgagatacc ggtgcatcac
    24481 gtttcctcgc tgcaagcttt ctttttcttt atcgaacaat ttgatcactc gctgcaattg
    24541 cccgctttgg actgcttctt ctaatagctc ataagattct tcaaaccatt gtcggcaata
    24601 cggacaagat aggttgatga attcgaccat ttttttagga gcattcgatg aaccataaag
    24661 gattcctttt tgtgtatttg ttttcgttgc atcaatcact gatatatcca tgttaaaaca
    24721 cctctttcgc ttatagttta tacatgtctg acaaaaaaag gcaacgattt agactgtttg
    24781 tctaaaaatt agatgaccaa ttcaaataga catatgtgaa atatttttat aaataaaaga
    24841 aaaaatggaa tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
    24901 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
    24961 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
    25021 tagtagatca atggtcagca gcaggaaaag aaaatgtatt cggtcaacca gtaaaagtag
    25081 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
    25141 cactgacaac tacatatacc gcttctcaag ggctgctttt gatgataccg aatatgtaca
    25201 agattgctgg ggaactactg ccaagtgttt tccatgttgc tagtcgggca gtgacgacaa
    25261 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
    25321 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
    25381 cttctattga aggagaagta ccttttttga atttctttga tggattccgt acgagtcacg
    25441 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
    25501 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
    25561 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
    25621 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
    25681 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
    25741 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
    25801 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
    25861 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
    25921 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
    25981 gtggtcgcta cggattgggt tctaaagatg tgactcctga ccaaatctca gcagtttttg
    26041 atgaattgca aaaagaccca agcatacgca aaaaacgttt caccataggt atcgttgatg
    26101 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
    26161 tccaggccaa gttttggggg tttggatctg acggaacagt cggagcaaat aaatcagcta
    26221 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
    26281 aaaaatctgg tggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttctg
    26341 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacgc accagcttac ctgcatagct
    26401 ataacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
    26461 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
    26521 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
    26581 tcaatacggc aatggaaacg gcatttttta aattagctga catcattcct tttgatgaag
    26641 tgattccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
    26701 tagaaaaaaa catacaagca atcgaccgga cgaccgaact gcttcatcaa gtcgcgatcc
    26761 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttatg
    26821 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
    26881 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
    26941 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
    27001 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
    27061 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
    27121 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
    27181 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
    27241 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
    27301 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
    27361 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
    27421 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgacaaatg
    27481 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
    27541 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
    27601 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggttg gtacacatgt
    27661 ttgagagcga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
    27721 gatcaattgt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
    27781 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
    27841 atgtacttgc cagcggagca gacgtgaata ttcttgtttt ggataacgaa gtctattcca
    27901 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
    27961 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
    28021 atgtggcgca aattgcttca ggtgccaatc aaatgcagac gataaaggct tttgaagaag
    28081 cagaaaagta tcctggacct tctttaatca ttgcctatac gccttgtatc acacatggtt
    28141 tgcttggtgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggctatt
    28201 ggtcattata tcgatataac cctgcaatgg tcgaaaaaaa caagaaccca atgactttgg
    28261 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
    28321 cctcgctaga gaacgcaaat ccaaatcttg caaaagcttt atttgaaaag actatccaag
    28381 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
    28441 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
    28501 cggaccttga agcagaagaa agaagagctg ccagagcaga acgacgtaaa aaaatggaac
    28561 aagctacaaa agaaaaaaag gaaaaataac agaaaaaata taatgaataa ttctctgaag
    28621 tttaaaaata tttattgcag agaaaaataa aacgcatact tgcaattttc ctgaaaatta
    28681 tgcgtttttc tcaagaaaaa tacgagcaga tcagcctcta aaattaaaac atatgatata
    28741 ctagagagga aatctcaaaa gagaagagag gctttttatg tcatttcaaa tcggggaact
    28801 ttttaatttg gactattggc agcaaatgtt ttccggtgac ttgttctctc tgaatttttt
    28861 ggtgaacata cttgatattc tggttgtctg gtatcttgta tacaaactga tccaactgct
    28921 gcgaggcaca aaagcgattc agcttctgaa aggtgtagct atttttattg ttatccgaat
    28981 cgtagcggaa atcattggtc tgcacacctt gtcttggctg atgaaccaag tcattacgta
    29041 cggggtgata gctgctatcg tgatttttca gccggaaatc cggagaggac tggaacatct
    29101 tggacgcagt tcgcttttca aacaatcaaa aagcgaacag caggaagatg agaagatgat
    29161 cttgtctttt gacaaagcca tccaatatat gtccaagcga aaaatcggtg cgctaattac
    29221 tatcgaacgg catactggat tagatgagta tattgagaca ggaattgcat tagatgcaga
    29281 tatcactgga gagctgctga tcaatatctt tattcctaat acaccgctgc atgacggtgc
    29341 agttattgta aaagaaggaa agatcgcagt agcaagcgcc tatcttcctc tatcagaaag
    29401 tatgttgatt ccaaaagaat tcgggactcg acaccgggca gctgtcggta taagcgaagt
    29461 cagcgacgcg attaccatcg ttgtctctga agaaactggg gatgtcagca ttacattaga
    29521 taatgaactg atggctggct tatcccagca agaatatttg gcaattttgc gtagagaact
    29581 gattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt caatctttcc tagacggtat
    29641 gactaaggga ggggatcgca aatgatctca aaagagaaac ggacaagcat tctttacagt
    29701 tttattgcat tgctgttcag tattgcgctg tacttcaatg caaatggcca gtccgttcca
    29761 aatacgcttt ctggaaatga agcgtataat caaacagtta ctggggtgcc aatcaagatt
    29821 tcttatgatt caaagcgcta ctatatccat ggatatgaaa atgcagtgac tgtcaaactt
    29881 tccagtgcaa accgagttca gttgaatgca gaggcaaacg aagatacacg gatgttccgc
    29941 gtcacagcag atatcactaa gctgggcgaa ggaacacatg aggttccgct aaaaattcaa
    30001 aatttgagca gtgctgtcac agcaaagatc gaaccatcga ctattacagt aacggttgag
    30061 aaaaaggtaa taaaagattt cgatgtggaa acgttgattc cttcaacgtt cacaccatct
    30121 ggatatgaac ttgatgatac ttccgttagt cctaaaaaag tctctatcac tacaggtgac
    30181 aaaacattgg cagaaatcaa acgagtagta gctgatgtgg acgcttcgat ggtcacagat
    30241 gatgggatta atagtgaggt cccaatacag gcgctgaatg cagcagggga agtattaagt
    30301 atcgtctctg atccagtgca agtaacagtg agagctgatt cgatcaagcc aagcaagtcg
    30361 gtacgacttt atgggataca gcaaggaaca ccagctgccg gggtagaaag ctatgatttc
    30421 agtttttcaa aactggaagc agaagtaagc ggctctagcg atttgcttgc atcgatcggt
    30481 gacagtattg ctgtaccgat caatgtctca ggtatcaatc atcgaacaac tcgtaaaatt
    30541 gaaataccag tagaagaggg gttaagtatc aagcccgaat cagttagtgt ggagattaca
    30601 cccgtgttgc aaacgtcaag cagtagctct gcttcgacga gcagtcggac acaagaaaat
    30661 acaagtacga gtagaagtat ttcatctgct gattcaggaa cggaaccatc gacaacaacc
    30721 atttcttcca gttcggaatc tgggacaagc agctcgacca caagtacaga agaaacatca
    30781 gaaacaagcg aatcaacaca aatgagcaga acaaattcct cagaaaatta agttaaagga
    30841 gtaattttac aactatgggt aaatattttg gaacagacgg cgtacgcggt gaagccaaca
    30901 aggaacttac gccagaactt gcatttaagc tgggacggtg cggaggttat gtcctaagcc
    30961 agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg gcgggataca agaatctcag
    31021 gtcaaatgtt agaagaagcg ctgatttcag gacttttatc ggtgggtatc gaagtgttcc
    31081 agctaggtgt gatctctaca ccaggtgttg cttatctgac acgtttgcaa aaagcaagcg
    31141 caggggtcat gatttctgct tctcataatc cagcacaaga taatggaatc aaatttttcg
    31201 gtgcagatgg ttttaaacta gtagatgacc aagagctaga aattgaagca ttattagatg
    31261 ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt gggaactgta gaagaatttc
    31321 cagaaggctt attgaaatat tctcaattcc ttgtacaaac gatcaacggt gacctttccg
    31381 gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc aacaagtgtt aatcgtttgt
    31441 ttgccgatct tgaaacagat ttttatacca tgggcacaaa tccagacggg ttaaatatca
    31501 atgatggcgt aggctctacc catccagaac gtttagctga gatggtcgtt gaaaaaggag
    31561 cagatgctgg actagctttc gatggcgatg gcgatcgtat tattgcggtg gatgaacttg
    31621 gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc taaatacttg gcagaaaaaa
    31681 atcgattgaa aaaagatacg atcgttacga ctgtcatgag taacttagga ttccacaaag
    31741 cggtggaagg aatcggtttg aaagatgtcg ttacacaagt tggtgatcgt tacgttgtag
    31801 aagaaatgcg caaaaacgat tataactttg gcggcgagca gtctgggcac atgattttct
    31861 tagattttaa cacaactgga gatgggatgc tttcaggaat tcagttactg aatatcatga
    31921 aacagacagg taagaaatta tcagagcttg cagcagaggt aacaatctat ccgcaaaaat
    31981 tagtgaatat ccgagtaagt gataaaaacg gtgcgatgga tgttccggca atcaaaaaag
    32041 tgattgaaga ggcagaagct gaaatgggat cagaaggaag aatcctagtt cgtccttccg
    32101 gtacagaacc attacttcga gtgatggcag aagcgcctac tgatgagaaa gttaattact
    32161 atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg actggattaa gagatatttt
    32221 cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg acactggtcc ttgttctttt
    32281 attattctac tgtcactgat ttcgctaaat tacgtggttt gtcaacgtcg aatccccgta
    32341 atagcgttgc gtaatatgca agtaattgag tcggtacgac tgtggttaga gtagttagat
    32401 aagggtggac agttggtagc accagctggt cactttcctt ttctaatccc tctgagacaa
    32461 taactagtgt tttagcgcca cggctttcga cttctttcag gtttcctcga gtatgcgcac
    32521 cagtcacttc ttcactgatg attccaatca ctggtgttcc ttcttcgatc aaagcaatcg
    32581 ttccatgttt caattcgcca gcggcaaaac cttctgcctg gatataactg atttctttca
    32641 atttcaaagc agcttcaaga gaaacgttgt agtcagcgcc acgtccaatg taaaaggcat
    32701 ttcttgtatc gctgagatat acttctgcca actcttctaa ataatctttt tgggcgatga
    32761 ccgattccat cgcattagca actaaactca attcatgagt gatatcgaat acaagagaag
    32821 tttcaaattc ttttttatca ccgatagctt tggctaataa agttagtaca gctatttgtg
    32881 cagtataagc tttcgttgat gctactgcga tttcgggtcc agcatgcaat agcagtgtaa
    32941 aggatgcttc ccgcgaaaga gtagaaccag caacatttgt aatcgtaagt gaagggtagc
    33001 cgagacggtt gatttttacc agaacttggc ggctatctgc tgtttcgcca ctttgactca
    33061 agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt atagccaaat tcactggcta
    33121 aatgaacctc aacagggatc tgtgtcaatt tttccagagt ctgtttacct gctaaccccg
    33181 catggtaact agttccgcaa gcaacgatat agattcgatc gctatcagac aatgtatcga
    33241 ccagcttttg atcaagagag acacgattgt cttctgttac gtatttttga gcgatacgac
    33301 gcatgataat aggctgttcg tcgatttctt tcagcatata aaaaggatag gtacctttct
    33361 cgatatctga agaatctact tgtgcttcga atgaagaacg ttctacgatg tcacctgcaa
    33421 aattttgaat aataactttt tctgatgtga cggtaaccat ctcttcatct tcaatctcaa
    33481 cgaattcctt cgtatgagca agcatagcca ttgcatcgct ggcaatcaca ttgaatccat
    33541 cgccgagtcc gattaatagt ggacttttat ttttagcgac gtaaataaca tcaggtgctg
    33601 tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac ccgcaaagct tttaaaaacg
    33661 cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag atgagcgata atctcagtat
    33721 cggtctcacc aataaagtgg tcatttgcta aataagcaat tttaagttct tcaaagtttt
    33781 cgattacgcc attgtgaacg agcacgaagc gtccactttg ggaagtatgt ggatgggcgt
    33841 tttcttcaga aggttgcccg tgagttgccc agcgtgtatg accaatacct atatttcctg
    33901 tagtatcaat atttattttg tttttcaaat tttgaattct gccttgtgct ttgaccagat
    33961 aatcagttgt gccatctgag acaaagatac cagctgaatc ataaccacgg tattctaatt
    34021 tttctagacc gtcaatcaat cctggtgtta cattttttaa tccaatcatt ccaacaattc
    34081 cgcacataaa aaacatccta ctttctataa tgaattcgta tgaatgcttc cagctgcagg
    34141 aaaatccttt tgttttatct ttgcagatat tttttgtgga ttttctgtag agtgcagtcc
    34201 ctagggccat ccgccgaatc tttcgataaa ccctctcctc gtcatctgtt tacacagacc
    34261 tggcgctatc ctaaaaggta taactatcct cctcttttgt tttttatctg gaaacattta
    34321 aaatcataac cttttgcgaa tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta
    34381 tatatcaaat gctttgtcaa atgctctttt tatgtcaaaa aataatgggt ttttatctat
    34441 catctttttc aggagttcgg tacaatagaa agtaatcgga tagaggagga gtaaaaatgg
    34501 attcaaaaat tgagatcatg acactgggaa tgttaaaaaa acaattgtcc aaattcgaag
    34561 catcagctgg tgtatcagat gatacgaaaa tatttttaga tactggctgg gatagcatcc
    34621 aagaaatcgc acctgatgcc ttggaagttg ctgaagctcg tgagtttacg gtagaagatg
    34681 aattgacaaa agagtcgttt tccgggtatg cgcgggaaga aaaagcagag cggttcgaca
    34741 cttctgagca gtcagaaact gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa
    34801 tgaataagag acgttgggca gcagttctga ttgctgcagg attatttgtg gtttcgttag
    34861 tttcagctag tttgactaag cctcaggaaa acgatcaaat tgagggattg aacagttggt
    34921 tatatgggga tgaagaacta gcttcaaatg tgttggaaga gggagacagt acgcaaaaaa
    34981 tcgtaaaatt atcagtagat ggaacgattg ctgcgggagg gtcttcaggt ttatttgctt
    35041 ctgaaggtta caatcataaa aattttatgg aacaactgaa ggcgattgaa gaagatccaa
    35101 cagtcaaagg tatcttttta gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg
    35161 aaatcgctaa gaaattggat acgattcgta aagaacatga tatcccaatg tatgtcagca
    35221 tgaaaaacat ggcagctagc ggtggctact atatttctgc tcaagcagat aaaatctttg
    35281 caacagaaga aacagtgacc ggctcaattg gtgtgatcat gtcaggattg aattattcag
    35341 gacttctaga aaaactgggt gtggaagata caacagttaa aagcggagcg ttgaaagata
    35401 tgggttcagg aacgcgacca gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg
    35461 acagcgcata tagtcgtttt gtagcaatcg tcagcgaggg acggaataaa tctgaagaag
    35521 aagtcaaaaa aattgctgat ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt
    35581 tagtcgatga attgggattt cctgaagatg ctttaaaagc tatgcgaaag gaacaacagc
    35641 ttgaagatgc ggaactagta gaatattctt ccacgccatc tgggtttgct agtacatggt
    35701 taggaagtaa actagcagaa ttccagggat tgaagccttc tgagactagt cagatcttat
    35761 ccgttcttga gaatctaggg acagcggaat caccaaaagc aatgtattat tacggaggtg
    35821 agtaagcatg tcagagcttt caaatgaaaa taaggaacaa gaaaaagata agcaacagca
    35881 gcctgctgta ggacctgact ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga
    35941 gcctttgacg cccgaagaaa tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac
    36001 aagaaatgaa ccgccaaaag caaatgattt tcctaagtat ttctatgcag gtttttttat
    36061 ccgaatgttt gctttcttgg tcgatctttt gtgtatcgga gctattacgc gtttaacact
    36121 tggtattgcc aaaaatctag gttggcttaa tttttcagat tcttatctga gtatctacgg
    36181 attcacagcc ttacttattt atctagcgta ttttatttta ttgacgaaat tcaatcatgg
    36241 tcaaacgatt gggaaaatga tctttggtat tcgagtcatc agctttgatg aagaagaact
    36301 tagctggtca accgttttag ttagagaagg tgcttgccgg ttcattctaa aatttccttt
    36361 gctgatgtta gggtatcttc cagcaatctt taatcagaaa aagcaacatc taggcgattt
    36421 tttcactaat acaagtgttg ttacgatcaa tctgatcaaa gcattcaacc aggaagtcaa
    36481 cagctgatga gcaataaaaa gcagtaacag ttgaaagcac cttctaaaag tcagatctaa
    36541 aatctagctt ttagagggtg cttcagttat ttttaattgt catgcttttt ttgttttgct
    36601 actattgaac atgagctgaa catgagcaag aggaggacac tgcgtgaagc cgattgattt
    36661 tccagaaaaa tatgagaatt taatgcgagt agcacaacaa gcactagcaa accagcaata
    36721 tcagcaggca aaagaattat ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa
    36781 tagcctgctt gttttttgtc tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc
    36841 actcttgcat gaaaaacagt atcttcaaaa tgaggagttt gctgaatttt attttgactt
    36901 gttaatcagt acacaagatt ttttatatgc tagaaaattg atagcaagta cagattttta
    36961 tgaagctttc gagcagcggg tcattgaaaa aatccaattt gcagaagaat tatccggaca
    37021 aatggagagg cagaagatca aggcactcca caataaatcg gaggaacttc cttcgttgga
    37081 acctgcacgt cagcttgcgt ctattgatga gatcgaacag cttccttatc atgagtttat
    37141 ccaaacagcg agtaaactta ttgtatcgcc agaggtccat attttagcaa gggcaaaatt
    37201 actggagaca cttaggcaat tgaacgaatg ccatcctgtt ttctatttga cgattgaaga
    37261 aaagttagtc aaagtcatac caaaagatct gccaaagccg cagcagcagg caagttacca
    37321 gcaattatgt gtattttcag atcgttacgg taatgaagat gcgctgctta gttccgtgtt
    37381 aaatgaagaa ttcacgttgc aatctgcaat tgtttatcct gtctatgata catacatcga
    37441 agacccgaag cgctggtttc aactgactgt agaggcgtgt acaggaaaaa cgatgtatga
    37501 aggtacagaa gatgaaaaac aagatttttt aaaaaaaaga gaaaaaattt tgcagcaaat
    37561 gatcttcttc cattagattt tatcagtgaa aaagataaaa atgttatgaa aaacaaggat
    37621 tctatccatt caatatgttt acaatttgaa agggttagtg tactatttaa aggtatgcat
    37681 gaaacgtttt tgatgtataa aatgaatttc taaactcgga gggaattaaa tgactgcaac
    37741 atttgaaaaa aaaggcacga atgatggtgt tttaaaattt acgattgcgc aagacgaaat
    37801 ccaaaaagga ttaacaactg cgtttaataa agtgaaaaaa agcttgaacg tacctggatt
    37861 ccgtaaaggg aaagtttctc gtcaagtatt caaccgtatg tacggtgaag aagctttata
    37921 tgaagatgca ttgaatgctg tattaccaga agcttatgaa gctgctgtca aagaagctgg
    37981 catcgatcct gtttctcaac caaaaatcga tgttgaaagc atggaaaaag gtcaagactg
    38041 ggtaatcact gctgaagtaa cagtgaaacc agaagtaaaa ttaggacaat ataaagatct
    38101 agttgttgaa aaacaagacc gtgaagtaac agatgctgac gtggatgcac gtatcaaacg
    38161 tgaacaagaa gcacaagctg aattagttat caaagaagat gctgcagctg aaaatggcga
    38221 tacagtagta atcgacttcg aaggctttgt agatggcgaa gcgtttgatg gcggaaaagg
    38281 cgaaaattac tcacttgaat taggttcagg ctcattcatt ccaggttttg aagatcaatt
    38341 agttggtcat aaatcaggtg aaagtctaga tgtaaatgta actttcccag aagattatca
    38401 agcagaagat cttgctggaa aagaagcagt cttcaaagtg acaatccatg aagtaaaagc
    38461 aaaagaagtg cctgaattag atgacgaatt cgcaaaagat gttgatgacg aagtatcaac
    38521 attagacgaa ttgaaagaaa aatatcgcaa agaattagta gaaacaaaag aaaaagcagc
    38581 ggacgatgca aaagatgaag cagcgatccg tatggctgtt gaaaacgcag aaatcgttga
    38641 attgccacac gtgatggttc atgatgaagt acatcgttca atggacgaat tcttgaacaa
    38701 catgcaacgt caaggtatct ctcctgaaat gtactatcaa ttaactggtt ctacagaaga
    38761 agacttgcat aaacaattcg aaggtgaagc agaaatgcgc acaagaacaa atcttgtgat
    38821 cgaagcaatt gcagctgctg aaaatcttca agcaacagaa gaagaaatca atgcagaaat
    38881 ccaagaatta tcagaaacat acaacatgcc aatcgaacaa atcaaacgcg tgttgacaga
    38941 agatatgctg gaacatgatg tgacaatgaa aaaagcaatc gaattgatca caggtacagc
    39001 gacagaagcg aaagaataga aatcaagtaa taaatcattt ctgaagaagc tacttctaga
    39061 aaattgttta ttcgaaataa gggactgtga cacgattttg tcacagttcc ttattttttg
    39121 ttaagagtaa aaaatgcaaa ggtataggtc agttgatcct ttgttaaaca ctttttcttt
    39181 ttactaaact atgttatgat agtaaaagac tgtaaagtct ttgatagttt cttgcatact
    39241 ttttgcaagt taaggggtga agaccatgta tgacaatcca agtagcaatg agaccgtacg
    39301 ctgttctttt tgcgggaaaa cccaagaaga agtgaaaaag atcgtagctg gtccaggggt
    39361 ctacatctgt aatgaatgta tcgacctatg taaagaaatc attgatgaag aattttatga
    39421 agaagctgta cgcgaattca ctgaagtccc taaaccacta gaaattcttg aagtattgaa
    39481 taactacgtg attggacaag atcgcgcaaa acgctcgtta gctgttgccg tatacaatca
    39541 ctataaacgt gtcaatcagc aagcacaaga aaacacagat gatgttgaac tgcaaaaaag
    39601 caatatctgc ttgatcggac ctacagggtc tggtaaaaca tttttagcac aaacattagc
    39661 aaaaacattg aatgtacctt ttgcaatcgc agatgcgaca agtttgacag aagctggtta
    39721 tgtgggggaa gatgtagaaa acattttgct taaattgctt caatctgctg attacaatgt
    39781 ggaacgtgca gaaaaaggga tcatctatat tgatgagatc gataaaattg cgcgtaaaag
    39841 cgaaaatgta tctattactc gtgacgtttc cggcgaaggt gtccaacaag ccttgctgaa
    39901 aattctagaa ggaactgttg caagcgtgcc acctcaggga ggacgtaaac atccacatca
    39961 agaatttatt cagattgata cgacgaacat attgttcatc gtaggtggtg catttgatgg
    40021 catcgaaacg attgtgaaga atcgtatggg cgaaaagacg attggatttg gtacacaaaa
    40081 ccaaaaactt tctgaagacg aaagtgtcat gcagcatatc attcctgaag atttactaaa
    40141 attcggattg atccctgagt ttatcggacg attacctgtt atggcagctt tagagaaatt
    40201 gacaacagat gatctggtac ggatcttgac tgagccgaaa aatgcgctag tcaaacaata
    40261 tcaaaaactt ctttctctag ataataccga acttgaattc gaacctgaag cgttgagagc
    40321 cattgcgaaa aaagcaatcg aacgcaatac cggtgctcga ggacttcgtt ccatcattga
    40381 agaaatcatg atggatgtga tgtttgatat cccttcaaat gaaaacatcg aaaaagtgat
    40441 tatcacaaaa gaagcagctg aattgtctgg tgagccaaca gtaatctata aagattcaga
    40501 gaaaaaagcg ggttgatgaa aagaggctgc cgtaaaagtc attttggctt ttgcgacagc
    40561 cgcttgcttt attcatcacc ttgttaagga gagagaccat gaaagtccat caagctgaaa
    40621 ttatcataag tgcagtggca ccaaaacaat atccagatac acaattgcca gaaatagctc
    40681 tggcaggacg atcaaatgta gggaaatctt cttttatcaa cacattgatt gatcgaaaga
    40741 atttagcccg tacttccggt aagccaggaa agacccagac attgaatttt tatttgatcg
    40801 agaacgcatt acattttgtc gatgttccgg gatatgggta tgcaaaagta tccaaaactg
    40861 agcgagcaaa atggggaaag atgatcgaga catatttaac gactagagac cagctacgtg
    40921 cggtcgtttc tttagtagat ttgcgtcata agccttctca agaagatatc cagatgtatc
    40981 aatttttgaa gtattatgat atccctgtaa ttgttgttgc cactaaggca gataagatcc
    41041 ctcgtggtaa atggaacaag catgagagcg aaatcaaaaa agcgttggat tttgatcgca
    41101 atgacacgtt tattctcttt tcttccgtta caaaacaggg aaaagaagaa gcgtgggcag
    41161 caatcgaaca agcaattgaa tcataaaaag aataaaaaag acttaccagt caaactcttt
    41221 cataaaagtt tggctggtaa gtcttttttt gagcttaact tacttttgtt tcttgtcttt
    41281 tttcttttca ttttgtggct gtggttttag aaatcgatcg atctcttctt ttggtgcaac
    41341 tgttttttcc ggatcgacag caaatgattc gaaaagttta tctaaattat ctgtacgttc
    41401 aaatttcaac cccattgtaa acacctcttt gtttcttttt acttagtcta tcatgtaatc
    41461 gtccaaattg gtttctaaga cataaagttt tagtttttct ttatcgttgg ttataattaa
    41521 attataaaaa gattttaaag aggtgagaaa atgattggcg aagataaatt attgatgact
    41581 atccagctca cagacttaaa tgaagaagaa aaaaaacagg aaataagcga ttgggctatt
    41641 ttgacacgtc tgttgatcca gccaacattt atccgctctt tcacacaaca agcggatccg
    41701 tatgatcttc gaaagcttca agaaaaaata atgctagcat cggtaagaga cgaaagttgg
    41761 ttagttgtag gaaatgacga gaacgaatgt agttttcgct tggaagataa tcaattattg
    41821 ataaaaaatg tattgtctat ctctgttttc aaagaaaagc aatttttgat tcgagacttc
    41881 attcagaaaa aaatgattgc tcatggggtg ttcgcctata tgcgagctta tagcgagttt
    41941 atctatcaca atacaaaaca gatctctcaa cggctattgt ttgagaaaaa ggatgaaatc
    42001 gaacatttac ctaaaatgaa acaacaaaac ggggaagtag tagtcgattg caaccaattt
    42061 ccaggatacg atctttttta ccaaggttta tgtttcactt cgtgttggga aatgtattat
    42121 agtcgctatt atcatcaaat catccctaaa ccgatttttc tcgacatcca acaagtagaa
    42181 aaagtgaagg aattagaaaa cgaagtcata tgtatccagc tttatcgaga cccctttaac
    42241 tggcaaaagc caaacaatca aatgtttcaa tcctatttta gggaccagct tgggtttgat
    42301 catcttgctt gggacaatgg tgtcggttta ttgaaaccgc cattcgttga atacatttac
    42361 actgaccata cgatccaatc tgtccagtat cagaatgcac aaatgcaacc tgttcctaaa
    42421 aagaatgctt cattttttgt aacaaaaagt tatgatattc aacaaggaga ttataaagaa
    42481 aggcgtgttc gcggaacact aaatgctcag gcgtattttc cttgggttga cgagaatcgc
    42541 tcgcgcatga tgtgctataa agttatcgat ccgactgttg ctttggataa tggaatcgaa
    42601 gcttattgtt attatattag ggaatatcta gaagtagagg taacagatga aaaatatcaa
    42661 gagtatcttt tatccttaag aatctatgtt ccttcagaat ccctgtcaga attaccgtta
    42721 aaggaaatca aacatagact ctctgatgtt accttcaaac gaataaagaa aaagcggcga
    42781 agtatccaat tcgatgtcaa gaaaggtgag cagcatctgc gtgtgcaatt tttagattac
    42841 cgagaattag aacaattgat tactttacaa aaaatatgag gtgaagaaaa tggataaaca
    42901 actctattat cgaataatgg ttccggcatc attattatcc gtctgtctct tttttgttcc
    42961 taattttcct attcgatcag tcatcggttt tttggtcatg tttggcggga tgggacttta
    43021 ttattatctt cttcataagg aaagaaaacc tgcttctgtt tatatgaaaa aaacaagaag
    43081 cgaaaataaa aaacgataaa acggcttgat gatagacaaa aaa
//
LOCUS       DANKHK010000012        41553 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000012 DANKHK010000000
VERSION     DANKHK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            34..711
                     /locus_tag="PW109_000598"
     CDS             34..711
                     /locus_tag="PW109_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510473.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            714..1841
                     /locus_tag="PW109_000599"
     CDS             714..1841
                     /locus_tag="PW109_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510474.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2041..2481)
                     /locus_tag="PW109_000600"
     CDS             complement(2041..2481)
                     /locus_tag="PW109_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2510475.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(2492..4525)
                     /locus_tag="PW109_000601"
     CDS             complement(2492..4525)
                     /locus_tag="PW109_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2510476.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4527..6230)
                     /locus_tag="PW109_000602"
     CDS             complement(4527..6230)
                     /locus_tag="PW109_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2510477.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6324..7274)
                     /gene="manA"
                     /locus_tag="PW109_000603"
     CDS             complement(6324..7274)
                     /gene="manA"
                     /locus_tag="PW109_000603"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2510478.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(7313..8338)
                     /locus_tag="PW109_000604"
     CDS             complement(7313..8338)
                     /locus_tag="PW109_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2510479.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHSVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8547..9416)
                     /locus_tag="PW109_000605"
     CDS             complement(8547..9416)
                     /locus_tag="PW109_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2510480.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(10120..12012)
                     /locus_tag="PW109_000606"
     CDS             complement(10120..12012)
                     /locus_tag="PW109_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510481.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(12009..13736)
                     /locus_tag="PW109_000607"
     CDS             complement(12009..13736)
                     /locus_tag="PW109_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510482.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13752..14402)
                     /locus_tag="PW109_000608"
     CDS             complement(13752..14402)
                     /locus_tag="PW109_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2510483.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14606..14902
                     /locus_tag="PW109_000609"
     CDS             14606..14902
                     /locus_tag="PW109_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2510484.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            15094..15714
                     /locus_tag="PW109_000610"
     CDS             15094..15714
                     /locus_tag="PW109_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2510485.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15806..16972)
                     /locus_tag="PW109_000611"
     CDS             complement(15806..16972)
                     /locus_tag="PW109_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2510486.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16984..17337)
                     /locus_tag="PW109_000612"
     CDS             complement(16984..17337)
                     /locus_tag="PW109_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2510487.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17465..18487
                     /locus_tag="PW109_000613"
     CDS             17465..18487
                     /locus_tag="PW109_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2510488.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18506..18667
                     /locus_tag="PW109_000614"
     CDS             18506..18667
                     /locus_tag="PW109_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510489.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18926..19360)
                     /locus_tag="PW109_000615"
     CDS             complement(18926..19360)
                     /locus_tag="PW109_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510490.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19470..21329)
                     /locus_tag="PW109_000616"
     CDS             complement(19470..21329)
                     /locus_tag="PW109_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2510491.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21447..22606)
                     /locus_tag="PW109_000617"
                     /pseudo
     CDS             complement(21447..22606)
                     /locus_tag="PW109_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(22913..24337)
                     /gene="araA"
                     /locus_tag="PW109_000618"
     CDS             complement(22913..24337)
                     /gene="araA"
                     /locus_tag="PW109_000618"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2510492.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24353..25051)
                     /locus_tag="PW109_000619"
     CDS             complement(24353..25051)
                     /locus_tag="PW109_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2510493.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(25008..26669)
                     /locus_tag="PW109_000620"
     CDS             complement(25008..26669)
                     /locus_tag="PW109_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2510494.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26887..28287
                     /locus_tag="PW109_000621"
     CDS             26887..28287
                     /locus_tag="PW109_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2510495.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28539..29618)
                     /locus_tag="PW109_000622"
     CDS             complement(28539..29618)
                     /locus_tag="PW109_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2510496.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29704..30663)
                     /locus_tag="PW109_000623"
     CDS             complement(29704..30663)
                     /locus_tag="PW109_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2510497.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30675..32186)
                     /locus_tag="PW109_000624"
     CDS             complement(30675..32186)
                     /locus_tag="PW109_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2510498.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(32208..32771)
                     /locus_tag="PW109_000625"
     CDS             complement(32208..32771)
                     /locus_tag="PW109_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2510499.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32777..33640)
                     /locus_tag="PW109_000626"
     CDS             complement(32777..33640)
                     /locus_tag="PW109_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2510500.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33654..34550)
                     /locus_tag="PW109_000627"
     CDS             complement(33654..34550)
                     /locus_tag="PW109_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510501.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34725..36014)
                     /locus_tag="PW109_000628"
     CDS             complement(34725..36014)
                     /locus_tag="PW109_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2510502.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35986..37503)
                     /locus_tag="PW109_000629"
     CDS             complement(35986..37503)
                     /locus_tag="PW109_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2510503.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            37701..38591
                     /locus_tag="PW109_000630"
     CDS             37701..38591
                     /locus_tag="PW109_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2510504.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(38664..39488)
                     /gene="nadE"
                     /locus_tag="PW109_000631"
     CDS             complement(38664..39488)
                     /gene="nadE"
                     /locus_tag="PW109_000631"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2510505.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(39500..40972)
                     /locus_tag="PW109_000632"
     CDS             complement(39500..40972)
                     /locus_tag="PW109_000632"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2510506.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12075 a         8647 c         7207 g        13624 t
ORIGIN      
        1 tatgttatta aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac
       61 atgaaacgtg gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa
      121 gtagctgctt tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc
      181 caactgctgc caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat
      241 acagattatc gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta
      301 tcggctgttg attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa
      361 actgttttcc aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct
      421 ctaggaatga aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg
      481 ttattcgatg aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc
      541 atcagacaat ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat
      601 atccaatcga tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc
      661 tcaaatatgg aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt
      721 tacttacatt tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat
      781 tgttggtcgt gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat
      841 tcgatgaact gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag
      901 atgagaatga gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa
      961 tcttacatgc aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg
     1021 aaaaagatat actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac
     1081 aacaaaaacg tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta
     1141 tttttgactt gccagcgcat ctctctttag caaattacta cgaaaatatt ttttcaggaa
     1201 tgatttctag ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt
     1261 atgaaaaaag aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga
     1321 aacatagcat ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat
     1381 ttttgatcgt ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg
     1441 taggaacgat cattggtcag gagatacgaa tcctgccgat gtatgagtat cttttccaga
     1501 gctttctgtt cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg
     1561 ctttatttga acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag
     1621 agtatcgctt gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt
     1681 cttatgttga ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag
     1741 aacaggttac tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat
     1801 tcataggtat gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg
     1861 cggtgacaaa aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata
     1921 gttttcaaaa gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat
     1981 ttttatcttg gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg
     2041 ttatatatgt ccatgttcaa aaatcatttg gacttcttca atggtttccg catcactgag
     2101 cgccattact tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg
     2161 atgttctttt tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt
     2221 tccaaagtcc acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc
     2281 atctttgtca gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc
     2341 ttgtctttcg atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca
     2401 ctggccgatc tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt
     2461 ttctgctggt agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt
     2521 tcgctaacaa ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt
     2581 cattcatgat gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg
     2641 gcgaaggcgc cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt
     2701 caatggtcaa cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc
     2761 tggatgtatg gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc
     2821 gcttaagcag ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag
     2881 gaaataagct gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa
     2941 aacgaccgag taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt
     3001 ctatcatcca ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat
     3061 gcgaaaaagc ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa
     3121 tatattttcc accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt
     3181 gtaaatcgat tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa
     3241 aatacctttt caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc
     3301 ctgcaatact gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga
     3361 ccatataagc gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt
     3421 tacggattgc attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg
     3481 tcagtgtctc ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca
     3541 ttttgtacaa agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa
     3601 tcagttgttt tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat
     3661 caaatgagtt cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat
     3721 aaagttgttt tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt
     3781 ctttgtttgc cagactatta tatgtctcat ttgtcagtaa atgatttttc gtcacacgct
     3841 ccatcgtcaa tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct
     3901 ttttttggat caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa
     3961 cgaatcgaaa aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt
     4021 cattgatttc taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt
     4081 cgatctcgta tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca
     4141 actgtttgat atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag
     4201 caagactcaa cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag
     4261 cagtggataa gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt
     4321 ctgatgcctc aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt
     4381 tcaatgagac ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata
     4441 aattcatcat tttttggata gatacaggtg acgggctact gagaaattct gctaataatt
     4501 gtttttctct gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca
     4561 ttcgtagcta cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca
     4621 gttacagcta gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct
     4681 attgattctt ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc
     4741 gtcatgccca tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca
     4801 ggcgacgttt tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat
     4861 tggatcgctt cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca
     4921 tagatttgac tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata
     4981 accagctgat ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg
     5041 attttttctg ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata
     5101 cctgagtcgc aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt
     5161 tctttgaccg ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa
     5221 atgaaggtca caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa
     5281 ggcgcattgg caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta
     5341 tctaaccatt gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga
     5401 tacggtcgca gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc
     5461 atcaatgctc cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt
     5521 aagatcccta aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact
     5581 gtttgtgaga attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa
     5641 aatagtactt ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact
     5701 agccaagaca caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa
     5761 acgattccaa ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc
     5821 atttcatatc cagccttgaa tttcacatgg aatctagaaa caaattgttc atatacgatg
     5881 ccggcaagcg gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc
     5941 aaccctaaag aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg
     6001 cctgaataac tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct
     6061 aatgtggtgt gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg
     6121 ataaacagac ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt
     6181 tgtttcatag acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc
     6241 agacttctat ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc
     6301 tgctgctttc agctatctgg gaactatttg cctggttcag acgcaatgat tgctagttca
     6361 ccttggaccg cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat
     6421 ggatatgttt ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta
     6481 taaggggctt gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa
     6541 aactcagttt ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt
     6601 aattctggtg ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct
     6661 cttgtttgtc cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa
     6721 ctttgctgtg tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt
     6781 acataaaaga aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca
     6841 tctttgatca tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct
     6901 gcaccaggtt cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt
     6961 ttcatcccat aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag
     7021 atcttgatca ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct
     7081 gaccatagtt catctaatct tttccctttg aattcgccat tttctactac actgacacca
     7141 tgaggatgcg cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca
     7201 aagactgaac gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac
     7261 ataggttcct gcataattca aactcctctc atttttcata gctattatag cattatttcg
     7321 gtaataccgc tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact
     7381 ccgcacgatg atcgacagaa tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt
     7441 ttatatcaat caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt
     7501 cttctacatc cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt
     7561 gatcttgcag taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc
     7621 ccttgttcaa atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg
     7681 cgttacctgc atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa
     7741 cctcacccga aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata
     7801 agctgttcat cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt
     7861 cttggtaaat ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta
     7921 cttcttttcc tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct
     7981 gagatagctt atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat
     8041 gacgcaaagt gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga
     8101 gttctatctt tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg
     8161 tcagtgaggc tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt
     8221 gggcaaagcc ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac
     8281 ctggataaaa tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct
     8341 ttttctcctg cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga
     8401 atcaaccttt tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact
     8461 attttctctt tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca
     8521 tgcgacaatc gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt
     8581 tcttttggct cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact
     8641 gtgtgctttt tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct
     8701 tccatgcttt cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta
     8761 ggcaaatcaa ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc
     8821 aatacatgga tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat
     8881 tggcgttctt ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt
     8941 gatagtttac tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct
     9001 ggaatttctc tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct
     9061 gggtctacga gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa
     9121 ttcattttac ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat
     9181 gaaaaacggt tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc
     9241 agcagcaaca tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct
     9301 cttttcaaat caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc
     9361 acccgaatgg ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt
     9421 cttgctcccc tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata
     9481 actttgattt tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt
     9541 aatgagtaaa aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     9601 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga
     9661 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta
     9721 gtaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg
     9781 agaaactggc ttttgaacac cgtttaccaa gaggttgtga gcctgccgtt taactccgag
     9841 tcacaaggag caatttcaaa aaacagcttc tcaggtttgt tgactattgt gacttgtgac
     9901 cgaggagttg gcaggagaac accgtttaag gaagaggttg taaaatcagc ggtttgacct
     9961 gagtattaga gatacaaaac ggaaaaatag cttctcatat tttgccgttt tttgagctaa
    10021 taccgaagga cagctgattg aacaccgttt attcggaaaa agggactgtg acacgacttt
    10081 tgtcacagcc cctaaaaaaa ataataaatt tgtactattc tatgaagctt cttcttcaga
    10141 aaactgactg ttataaagat ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt
    10201 tcctgtttct acgatcgaac catgattcat cacaataatg ttgtctgcat cgcgtattgt
    10261 agacaagcgg tgagcaacaa cgaaacttgt ccggttttcc aataggcggt tcatcgcttt
    10321 ttgaatcagt atttctgttc gtgtatctac actagatgtt gcttcatcaa ggatcaggac
    10381 atctggattt gctaagaatg cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat
    10441 attgcttgca tcctcgttta atacagtgtt atatccttct ggcaatttac gaacaaaatc
    10501 gtctacgtga gcagctttag cagcctcaat gatttgttcg tctgttgcat cgtcgttccc
    10561 atatttgata ttgtcataga tacttcctgt aaacagccaa gtatcttgca gtaccattga
    10621 aaactgtgcg cgcaattcat cacgtgaaag atcacgggta tctacaccat cgtaacgaat
    10681 actacctcca gaaacatcgt agaaccgttc aagaaggttg atcagtgtcg tcttccctgc
    10741 accagtcgga cctacaatag cgaccatctc accaggtttg acatccaagt taaaatcagt
    10801 cattagcaag ttatctggtg tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg
    10861 tgtatctttt tcttctggaa ggccagattt ttcattggac atctcttctt catccaacac
    10921 ttcaaatacc cgttcggctg aagcaactgt tgattggata gtgttcatca aacttgcaag
    10981 ttgagtgatt ggttgagaga actgtgttgt atattgcaag aacgcttgaa catcccctaa
    11041 tgtcatgttc ccatttgcaa cttttactcc gcctagtaca gcgacgaaca catagcctag
    11101 atttttaacg aaattcatca acggcatgat gattgccgaa ataaattgag ctttccaacc
    11161 agcggcatac agattctgat tttctttttc aaatacttcc tggtcttttt cagtatgatt
    11221 gaatgtttta acaatggtat gaccaccata tgtttcttct acttggttat tcaataaacc
    11281 taaacttttt tgctgatctg cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat
    11341 gatcaagctt aaagggatca tcaataacgc gactaacgtc aattgccagc tgattgaaag
    11401 catcatcacg atgaccccaa tcaatgtgac tgcactagtt acaaattgag tcaaattttg
    11461 ctgcagcgtg cttgcgatgt tatccatatc attgattgct cgagacatga tatccccatt
    11521 tgtatgtgta tcataatagg aaatcggtac acgattcatt ttggcttcca aatcatgacg
    11581 taattcataa actgttcgtt gggaaacacg tgtcataata aattgctgta aaaagttgaa
    11641 tacagcagaa atcaaataca tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa
    11701 gtctatcgga aattttgtta cttgttggcc ttgttgcatc tgttgagcgc cagccattac
    11761 acctttgaat atttctgtgg ttgctttccc caagaccttt ggtgtttgga tttggaagat
    11821 cacggctgct attgctagta caaatacagc gatgatcgca accatacgtt tagacatata
    11881 gccaaacaat cggcgtaccg ttccccagaa atttttaggc ttttcacctt ttacaccgag
    11941 atttctgcca ggtccaccat gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt
    12001 tgttttactc atgctaagtc ctcctctctt agttgtgaat gaacgatttc ttgataagtt
    12061 tcgtttgttg ctaatagttc ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc
    12121 acaaggatca tatctgcatc cattactgta cttactcgtt gagcaactaa aacggtgata
    12181 ctttctttca tattcgtttt caatgcttgg cgaagattgg catccgtttt gaaatccaac
    12241 gctgagaagg aatcatcaaa cacatagata tctgctgttt tgactaatgc ccgtgcaatt
    12301 gccagacgct gtctttgtcc accagagaag tttcctcctc cttgttccac atggctatct
    12361 aagccatctt ccatctctga aacaaaatct ttagcttgtg cgatttctaa tgctttccaa
    12421 atcatttcat ctgttgcatc cggcttacca tactgcatat tgtctcggat cgttccagag
    12481 aacagaactg ctttttgagg aacaaagccc attaattctc gcaaattata ttgagtcatg
    12541 tcactaatgt ccgttccatt tatttggatc gaaccagatt cgatgtcata taatcgaggg
    12601 atcaaattga ctaaggtcgt tttacctgaa ccagtccccc ctatgattgc gacgatctcg
    12661 cccgacttgg cttggaaatc aatatcttcc aatgcaagtt tttctgctcc atgatatcga
    12721 taattgacat gattgaaggc caatgttgcg ttttttccag caaaagaaac tgttttagga
    12781 ttttcagggt catggatacc gattttttca tccaacactt cattaatacg gtcagctgaa
    12841 gcttgtgcac gaggcaccat gataaaaata gcggaaagca tcataaagct gatcaagatc
    12901 tgcatcgcat aagtcataaa tgcaatcaga ttaccgactt ccaaagtcat atctgctatg
    12961 tagtgtccac caaaccaagt gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt
    13021 ggcatcatca atgcgacgat cgtattaact ttgattgccg tatttgtata atctttattc
    13081 gctaaatcaa aacgattttc ttcaaagcgt gtcttatcga atgctcgaat cactctgacc
    13141 cctgtcaatc cttctcgaaa taccaggttt aaacggtcag ttttcttttg catacttttg
    13201 aataatggaa cagcaaagta catgattccg ccgatcaaaa taatcattac tgggatgaca
    13261 actaagaaga tttttgtcaa ctgatgatct ttttgataag caagtatgct cgcgccaatc
    13321 aacgtgattg gtgcattgat catcatacgt aaaaacattt gtgtaaccat ttggatttga
    13381 gtcacatcat tggtcgttct tgtgatcaaa gaggccgttc catatttatc aaacgcatct
    13441 tttgtcagac tctctgattt tttatagaca tccgtacgca attgcttacc cagtttctgt
    13501 gattcacgag tagcaaaaaa tgtatttgcg atagcagcta agatactgat caaagaaaag
    13561 ccgatcatca caaaacctgt atgccaaata taatcgacat ctccttgcgc taccccttta
    13621 tcaataatat tggaggtaag tgtcggcaaa tataaatctg ccagtacttg gatcaccatg
    13681 aaaactgcag ctgcaattgc tgaagtcaag gagatccgtt ttacgatttt tagcatctag
    13741 ttcccttctt tctatttata tactccgttt ttcagccaag aaagtttcaa attgaattca
    13801 ctgattgctt gctctactga gtaatcttca gatgttgaaa ataagcgata cgaatgcgca
    13861 attgttgtaa atacagtatt catcactaat tggatcagta attccaattc atgatcattt
    13921 tttactttca gcttcgtccg atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt
    13981 tctttatgtg gatcgtgagg cgttttttgg cgactcgtat gaggagcgac ttttcttgag
    14041 gcacggtaat ccatgttcat aaacaagtta cgataaaaag aagcattctc gccttccaaa
    14101 atctcataaa tcattttaga aaaataggat tcgaatcctt tgaatagatc accatcggat
    14161 tctttcatta gttgtaacat atctttagag ctgtcacgtc tcattgtctc aaagtaataa
    14221 aaataaagat cctctttatc ttcaaaatac tgatagaaac tgcctctagg gatatccgct
    14281 aatttgacga tattagcaat cgaagcttct tttaagggta ctctagaaaa ctcgattctc
    14341 gcagcctcga ttagtctttt ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc
    14401 atatggattc tcctttcttt caagatgaca cattgtcata tgacgctatg tcattataac
    14461 acctcctacc tgttttgcaa gacatttgca tagttttttt cattatcatc cgatacatta
    14521 aaaaagtgat aagaggaatc cttccatttg aattaatgag agttgcccat taaaatagcc
    14581 acatactaaa caaaggatga gataaatggc ccaatcaatt agtgtccaag acttcaataa
    14641 attgcctctc gatgctgaca gcatcgtttt agatacaaga aataccttag attacacaaa
    14701 aaaccactta agccactcgc tttctatccc cgctgctatc ttgccaagac aattaaaaaa
    14761 tctagatcca aagaaacttt attatattct aagctataca ggcaggcgat cagaagcatt
    14821 agccgatcaa cttactgcta atggttttcg tgctgtttat atcattggtg ggatggaagc
    14881 tcttcagtcg agtgttgctt gaatattgct tgttagattc aaaagaggct gtgacaaaaa
    14941 atcgtgtcac agcctctttt ataccctgtc tttttttagt tatttattct tcaaccttat
    15001 caatatgact tgatagcctt ttctttttcg agtaaactga ttttgttagt catagttata
    15061 acaatcatag caataaaagg agaaggtaag catatggcat ctgttttagt agtcaaaggt
    15121 catcccctaa ctgcagaaga atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt
    15181 tataaagaaa atcatccaga tgatgaagtg actgttttgg aactttatcg cgatgatatt
    15241 ccggaaatcg atgaagaatt attatcaggt tgggaagcat tacgtgctgg tgcagaattc
    15301 acttccttat cagaaagtca acaacaaaaa attgctcgtt tcaatgaatt gactgatcag
    15361 ttccttgctg ctgataaaat cgttatcgca aacgccttat ggaatctaaa tatcccaaca
    15421 aaattaaaag cttggttcga taccgtcaat gtagctggaa aaacgttccg ctatacagaa
    15481 aacgggccag aaggattagt gacaggtaaa aaagcactgc acatccaatc taatggcggt
    15541 gtatacaacg gacaagactt tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc
    15601 atcgatcaag tagatcagct attcatcgaa ggaatcgatt atgaccctga ccgtgcagat
    15661 gagttgatgc aaaatgcatt agataaagct gctgctttag gtaaatcatt ttaaaaattg
    15721 agataaatgc atacaaaaga gacagaggaa aaaatcttca aagcagcatt tgtgccttgg
    15781 atattttccc atctgtctct ttttgttatt ctttctgctt tttcaacgga ttttcttcat
    15841 aaagagaaga gattgttgtg taccattcaa aaacatgagt aatcactact ttagctgctg
    15901 cataaacagg aatccccaag atcacaccga tgattccgaa caatttccca gaagtcagca
    15961 gaactaataa aatcgttact ggatggattg ctaactgatt ccctagtact aatggagaga
    16021 tcaagcgtcc ttctattgtc tgctctactg caaaaacgat cagtactttg atgatcatga
    16081 atggacctgc tacaatggct aaaaagatag ccggaatcat cgctaaaaat gatcctaagt
    16141 aagggatcag gtttaaaaaa cccgcaataa tccccaacgt gacagcatac tctaaaccaa
    16201 tgaccgcaaa accaatcata aacatgattg ccacagcaaa agccacagtt agttgtcctc
    16261 ttatataaga agaaacttga ctattcattt cactcagcac attcaacgtc ggtttttgca
    16321 tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg tttaccgtct ttcaatagat
    16381 aaaataagat aaacggcatc gtcagcagag ccactaaaat agtcgctacc gcgccgacaa
    16441 aacttccaag actctgaact gtactcttag atatatcttg gatcatatcg cctgccgaat
    16501 tcatgaactt atcacctgct gtttccaact gttcgcgaaa ttggttgaac agcgggtccc
    16561 gcaaaatctc ggttaattta ttatcaatcg tatctacata ttgagggaag ttactgatga
    16621 agctcactgt ttgttcctga atcttaggga tgatcactac gctcccccat gcgattagtg
    16681 caacaaccaa aacaaacagc ccaatgatac tgtaaagcct tggtactttt cgcttttcaa
    16741 agtaatcaac taccggattc atcaagtaat acaagattcc tgctaaaatg ataggcagac
    16801 ctacgattgc taaaaattgc cagattggtg caaacaaata tgaaactttt gtaaataaaa
    16861 agacgatcag caatattaac aatacaacaa gtaaggctgt gactacttga ttatttaaaa
    16921 accagcgcca aaaccacgat accggtctgt tcttcttatt ttttccttcc ataattcctc
    16981 ctgttagtta taaaaaattt ggcttcccaa accaatagca ggcagtctat gcggatgaag
    17041 atagattcca tttgcaattt gtccttcttc tggacaagca tcaaaaatca atgatacgtg
    17101 agctactgtc tttaaattcc cattggccat cggaccgaca taatcgatcg tatatacctg
    17161 atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc
    17221 ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat
    17281 gggttcttcc ttgtttattg cttctgtccc aatttctgta atctttgcct gaatcattgc
    17341 actctctcct ttaattgctt tcaaaaacag tataacacat tctcattctt tagccgtgtg
    17401 gaataattat gtgatagaat aaaaacgaaa actatttcat tacaattagg aggaatcagc
    17461 tcatatgatc gaaaagataa ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta
    17521 tacgatcgac cttgatacag aaaaagaaga attagccaac ctttcattag ctgttgaaga
    17581 aaatagccct acctatgtag caaaaagtaa agctggcaat ctttacacag ttactagtgt
    17641 ggatggatta ggtggtgccg gtgcgtacga taaggatttt cattttttga atgcggtaac
    17701 agagtctggc gctcctttgt gttatgtagc ggttgatgaa gcacgtcaat tactatacgg
    17761 ggcgaactat cataaaggtg aagtaaatgt ctaccatata ttaggagatg gcgaactgaa
    17821 agcagcagat tcaatattcc atcaagaagc gacaggtcct cataaaaacc aagatcatgc
    17881 gcatgtccac tatacggatc tgactcctga ccaacgatta gtcgtatgtg atcttggtac
    17941 agatcgtgtg tatacttatg atgtgtccga aaacggcaaa ttgaatcttg taactacctt
    18001 cactgcagag ccaggaacag gccctcgcca tcttgtcttt catccaaatg gacaattcgc
    18061 ttatttattt ggtgaattag acagcacagt ccgcgtgctt tcttataata aagaaacagg
    18121 tgcttttgaa gaattacaga aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg
    18181 aggggcagct atccgcattt caaatgatgg caagttcctt tatgcttcta accgaggaca
    18241 taactcgatt gctgtattcg cagtttttga agatggttcc cacattcaaa acaagcaaat
    18301 catctcaaca gaaggggatt ttccccgcga tttcgcacta gatccaagca ataattttgt
    18361 cgtgtgtgcc aatcagaaca ccgataatct gacattatat cgccgtcatc ctgaaacagg
    18421 attactggaa ttactgaaaa aagatgtcta tgtaccagaa tgtgtctgtg tttattttga
    18481 tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac gtattagaca gactacggga
    18541 agaactaaat attcctttct ttgatgggaa aatcgaagat aaagagtata gcgaagagga
    18601 atatcagaaa ctcaaagccg atttacagaa ctactttgaa caatatgtac agaatgtaga
    18661 aaactaatca gctttccata ctatctgaaa agaacatcag gacaagactt ttccgtctat
    18721 cctgatgttc ttttctacaa ataaaaaacc gccgagatgg ttagactcga cggaaaagga
    18781 gtatttactc gattttgagt aagtacaagg ttgttgttgg ttataggtat gtcattatca
    18841 taaaggattt tgatcactct atcataaagg taatattaca tttatattag ataactctag
    18901 tttttattgt tatcaaagat ttcttttatt ttttcaacaa caaattagtc attttttgga
    18961 tggcagctgt caattggtct gtcgcttggt cttgttttgc ttcagatgta ttgaatagat
    19021 cttcatctac ccctgattca agatcgatac tttggatcat ttcttcacag cctgctacat
    19081 atgaaagata agctttttca aaatttttat gaatgcccat tgcttttgct ggtggacgca
    19141 aagtactgac tttcataagc attgcccgat atttttttgt tccttcttca aataacagga
    19201 tgatctcttc aagagtcgat ttgtccagtt cacttgtttt tccttcatca attgcgtttc
    19261 tgatcacttc atatttcgga tgcattcctt ctccgattgc ttctgtttcc ttgacgattt
    19321 gatcgatgga ctgcacataa tagccaatat tcggtaacat gttcgttttc ctactttctc
    19381 cattgttgtt attatttatt ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa
    19441 tttaatggtt tctatacagc tcgccattct tattcgtgct caaagaaacc atctgctaaa
    19501 ctatattgtt tccctgcgac aatgtcatat tggatcaatt gatattcgtc gtaaatctct
    19561 tgttgcgagc gattgaattg cgcttcttta taccactgtc cgttttgata gtacagttct
    19621 cgttcaaacg ccggaagttc ctgttctaat gataaaagca gctgataaaa gccatttgtt
    19681 gtctgattcg tctgttccat tagattcgct gcaaaataaa acggactggt gattgcatcg
    19741 tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca taagaaattg tgtttcatgc
    19801 aatgtttgtt tgtcattttt tgcttggatc gtatctgaat agatccctgg aagatgatcc
    19861 ccccaaaaaa caactaaagt ccttctagaa agattcttca attcttccgt gaactgcttc
    19921 agtgccgtgc tgctatagga aatatcttgc agataattct ctaagtcaag tgtcccactt
    19981 ccatctgaag tttttgcagg atagtctaga ttatcatact ttccatcgta gggcatatgt
    20041 gtctgcatcg tgactaaatg aatgaactga ggtgtttttt cttccttgag aagtgtcatt
    20101 acttctttgt aagctgactc atcagaaata tatggattat tctctatcgt atccgtatag
    20161 gtcatcgtac gctcgctgat gaactggtca aatcctaatg tctgataaac atcctctctt
    20221 ttatacatcg aagtattgta tggatggatc gctgtcgtct ggtatgattg tgcgtttaac
    20281 gctgaaacga tagaaggaag ctgatccatt tttggtacaa gcatcgtgta aggggtggtg
    20341 agttgcgcat taaataacgc catagaaaaa ccagtcaatg cttcaaattc gatatttgct
    20401 gttccaccgc catagttttg agaaagcata ttgccactat atgtttgatc agctacttca
    20461 taataatctg ccaatggctc accagatact tctacgccgt ttaatcgact aggatctgag
    20521 aaactttcac tcatcacgaa cacgatgttc ggcgactctt cctctacagt tttatttttt
    20581 tcatcggcta gcttctggta tttttcagtg atttctttga tcttttcttt cgaatatcct
    20641 tcaggttcat ccattggttc taccttcaga ttgtacaaaa acccaccgat aaaaccagtg
    20701 ttatagtagt tcattttctg gctgtaaggt atccatagtg ccgttttatt atatgctttt
    20761 cgcaacaaat tatcgggatt gttgaaatta ctgatatacc ctagcagtcc aattgccaca
    20821 agcagtgtcg ttaatcggat caattggaat cgccttcctt ttttcaggct tcgaaacatg
    20881 taccagcaaa acaatcctat cacggttcct gcggcagcta agatcactgt aaacagcata
    20941 gtgccggtca tatctttcag caatccgatt tcagtgatca tcttcaaatc gtctggataa
    21001 atcggttctt gccgataact cattttcata taatttgcaa aacctaatac accgatggct
    21061 acgacatacg taagggaacc taaagggaca gatcctgcta gtgcaatcag aaaaataaga
    21121 ataatcagca agacgagaca ggctaagaag aatttttccg tatgccaaga aaaagcaaat
    21181 ttcaacgcaa ggtctacgga tagatcattt tgacaccact gcaaaaataa attactaaaa
    21241 acaacaagaa aaaagatact gatgattgct gctgttcctt ttaaaatctt tttctgtttg
    21301 gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag
    21361 aatgttgctt ctcttatttt agttgtacga aattcgaaag taaagaacgg aaagaagaat
    21421 taatgaaatc caaatattct ctttctttat ggcctttctg gtttctttaa taagcggacc
    21481 tttcgtactc cagaaatttt ttctagctgt tgtttcactt gattgagctg ttcaaaatca
    21541 gtactgtcta tgtcaatcaa tatgtagaca tgctcattct tatggttacg cgatatatct
    21601 gcgatcgcta gctcgttctc attcagcaga gaaaatatct ctgctaatat attggttttc
    21661 ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa acattcgtgc ggatggataa
    21721 ttgaccgctt ctctaaccgt accgaacaaa agataatctt ttaaggcttc aactgccaaa
    21781 cgtccgctgt ctgccaaagc ttcctgagtc gatcccctag atgaggcaac ataagtatac
    21841 ggtcattttc taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt
    21901 tttccaatgc ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa
    21961 gtacagcatc ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt
    22021 ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg
    22081 cttgccgaac ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta
    22141 agctatagca acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg
    22201 ttttgccttg caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa
    22261 tattaggacc tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata
    22321 agagcaaaca gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca
    22381 tgacgatcgt cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag
    22441 ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa
    22501 taactattcc ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt
    22561 ctatctcttg ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt
    22621 actaacaagt atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga
    22681 agcaaaaaga gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt
    22741 actgccataa gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg
    22801 tgttccgtta gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt
    22861 cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa
    22921 taaccactgt ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat
    22981 ttagcgagac aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat
    23041 ggaaattcgg tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg
    23101 ttggttcaaa ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca
    23161 tcgatacgac taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc
    23221 tatctcccat tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat
    23281 ctacttccat catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat
    23341 aatcttccat gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg
    23401 ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta
    23461 cagctagtcc aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt
    23521 tatagcctcc ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta
    23581 catgttcctc ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt
    23641 actcttcaaa taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat
    23701 ctccgatacc aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc
    23761 cttctgtgac acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac
    23821 tttcattata ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc
    23881 gtttccagta gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat
    23941 attcgcggtc accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc
    24001 aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg
    24061 tcccgcggat ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta
    24121 cttcttctcg ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta
    24181 attcttgaag gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt
    24241 cttgtgcatg tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta
    24301 caacaaacca aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt
    24361 tttgtccgta atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt
    24421 gaggcagctc ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt
    24481 catctagtac gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac
    24541 cgtgtccgtg taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg
    24601 tttctatgat aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca
    24661 aaaatcgagc acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag
    24721 gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc
    24781 ctccgatttg aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat
    24841 tgagtttacc ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca
    24901 tctcttcata actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag
    24961 cgctcacatt tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt
    25021 ggtatacttc ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga
    25081 tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag
    25141 cctgctattt cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct
    25201 tctgccaaga agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac
    25261 gcgattcccc acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata
    25321 gcagatgcta gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact
    25381 aaacgatcaa tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca
    25441 aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta
    25501 cgtacaaata atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca
    25561 taagaaagta atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg
    25621 aataatttat ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg
    25681 acccaagcat tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct
    25741 gaaggtgtag tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg
    25801 atcatagcga aggcggaagt accagccgaa atgttccctg tccctttggc tacactgttc
    25861 gtagcgacca taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc
    25921 aaatttcccg tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct
    25981 cctgctacta gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg
    26041 attaactgac caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata
    26101 ggaaacatgc ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg
    26161 acataacctg caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa
    26221 atagattgat acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc
    26281 gttaatattc gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt
    26341 aactgttcct tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac
    26401 ccgattgtcg taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa
    26461 ctgacgcgca gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt
    26521 ttgttttccc actcataact accagcggca ataggagtaa aagatgaatc aatcaataca
    26581 gctttgatcc tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct
    26641 aacatacttt gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc
    26701 tgatcaaacc gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat
    26761 gtcaagcagt tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta
    26821 taaaatgaaa ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga
    26881 atacttatga aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt
    26941 ggcggcattc tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag
    27001 catgattggg gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg
    27061 ttaggtgcga tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga
    27121 aaaatgattc ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc
    27181 cctcacaatg gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc
    27241 gctgcatctg ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga
    27301 agattgtcag ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca
    27361 gattatttgc taaaagggtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct
    27421 gctgtccccg cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt
    27481 cttatgcaat ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca
    27541 aaagaagctg aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc
    27601 ggaacttctt ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt
    27661 ggtgtagcag tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg
    27721 atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa
    27781 ggaatcattt tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg
    27841 cgaactttat tgaaaatagg cggtagcgtg atgggacttt ctttcatcct accagctgtt
    27901 ttaggaactg tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt
    27961 tatgtcgcat tttattcttg cacatgggct ccactgacat gggtaatcgt aggcgaaata
    28021 ttcccactag ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt
    28081 tctttcctag ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt
    28141 ttcggcattt ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca
    28201 gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct
    28261 aaacgtattt ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa
    28321 ataagaagga accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat
    28381 gtttgatgaa tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt
    28441 ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt
    28501 aaccgtagga gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact
    28561 gtttcccgtt caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca
    28621 atcgttgtga cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat
    28681 gttgtcaatt ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat
    28741 aggtcatctg gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt
    28801 tgatcgttat agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt
    28861 ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa
    28921 cgcacattat actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga
    28981 tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct
    29041 gccataaaac cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa
    29101 gcagcattga tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag
    29161 tttgggttat actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca
    29221 atcattttct tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc
    29281 aatgaatacc cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata
    29341 taatcagaga gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt
    29401 tctttcttat agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc
    29461 tcatttacca acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct
    29521 tgcagcttga attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt
    29581 tgttttatct cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg
    29641 tacgtacata tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg
    29701 tgctcataag gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt
    29761 aaagcattgc gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt
    29821 ctctggtcga gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga
    29881 gttgtgacca ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa
    29941 tttatgccat gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat
    30001 ggcatagttt tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt
    30061 aaggactgct ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat
    30121 agaaaggagg atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag
    30181 attagaattc ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg
    30241 agaagagaaa gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt
    30301 ttctatccaa tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg
    30361 atgatgagat tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt
    30421 tccattgata taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg
    30481 agcctgccat acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct
    30541 taattcaatc acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg
    30601 tttatatacc caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat
    30661 cataacaaat ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc
    30721 cctacattta attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt
    30781 tctggttctt cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct
    30841 ccttgatcta gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga
    30901 ttgatcaaga aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga
    30961 acatttttta tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga
    31021 cctttagcat atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt
    31081 cctttagcat ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc
    31141 tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct
    31201 tccatagtaa agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt
    31261 tccggttggg catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc
    31321 aaatatcctt ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga
    31381 taagcttttg caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag
    31441 tcttcttcat catacttatc aatatagtta tgcaaagcta aataatctac attctcataa
    31501 gcttcttcta atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt
    31561 ccaactaaga ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt
    31621 gctaacctac catattcata agcactctta gcccccactt gccatggacc gtctagctca
    31681 ttccctaaac accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa
    31741 tcactccaga aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca
    31801 attccttttg ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat
    31861 ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt
    31921 ttttttggtc gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag
    31981 tttcctccag gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct
    32041 tgtctaaacc cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg
    32101 acacttccca tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta
    32161 aaatcctctc tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt
    32221 cctatagtca agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa
    32281 ggagggaaaa gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt
    32341 ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata
    32401 tctttgtttt cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa
    32461 actacatatc cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga
    32521 aaataaataa tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt
    32581 ccttgaaaga aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca
    32641 taaaaaacag cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag
    32701 agaataccta ataagatgta atcagaaacc acaatcaact gtccataaag tttagtctca
    32761 actattttca tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt
    32821 tctgtgcaaa aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta
    32881 caggtccata aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc
    32941 tcatttttga actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca
    33001 gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa
    33061 aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag
    33121 taataaaaaa ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca
    33181 aacctgcata agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga
    33241 agacttggaa tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt
    33301 tgaaacgcat acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca
    33361 ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc
    33421 aaaccttaat atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga
    33481 taatgtcttt ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa
    33541 ccaacaatat aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc
    33601 gactattcac agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc
    33661 atttgttttt ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg
    33721 ataaacaaaa ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct
    33781 cgactgtaaa tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca
    33841 acatatactt ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct
    33901 aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca
    33961 tccatttgag cagattcata caaagaggga gagattccct gaatagcagt caagagaatc
    34021 gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg
    34081 gttggatcgc gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc
    34141 gcccctacat tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa
    34201 ataactggaa tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat
    34261 gctactgctg ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat
    34321 aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca
    34381 aaacttacaa aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat
    34441 gttcctaaaa gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac
    34501 aaatacgcag cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc
    34561 tcatctttca ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc
    34621 gaacaatcgc tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag
    34681 atatctgtat cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat
    34741 tgattgatgg catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat
    34801 ttttcaatca gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt
    34861 aaatatggct ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt
    34921 acttcttcat cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat
    34981 tttttaccaa tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta
    35041 ttattatctg tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg
    35101 tctgaatcac tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat
    35161 ccgttattta accatggccc attgatttca atggctagtt gaccagcatt cattaaattg
    35221 tccatttcgg cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg
    35281 atcgctttta acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg
    35341 ttcactaatt taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta
    35401 ttgaagacaa aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc
    35461 aattcttccc aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca
    35521 ttattccaat acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct
    35581 acttcaccta attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc
    35641 cagaaatcgt ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa
    35701 tttaatgcaa taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc
    35761 aaattggccc aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta
    35821 aattcattaa cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa
    35881 gtcaaagtca cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct
    35941 aaaatacctg ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt
    36001 tgttcctcct tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa
    36061 acaattgatt ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc
    36121 acttttagga agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac
    36181 tccctctaat gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt
    36241 aactgcaaat atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac
    36301 ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc
    36361 ttgtccatac acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc
    36421 gtttgtttct gtcctgattg gtgcaataac atttaccaat tgagcaagac aagcaatctt
    36481 tactctatct gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc
    36541 aaaattatag ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc
    36601 ttgttccgct gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt
    36661 actatgtttc ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc
    36721 catgtctaag gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg
    36781 atgcagagac aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc
    36841 accaaatgta ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc
    36901 tacgagtttc atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt
    36961 atgtccaatc tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa
    37021 agggctctct tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt
    37081 acaatattcg actaaattac gagctgcatc tactccacga gtacccagat tgacagccat
    37141 attgatttcg gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt
    37201 cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat
    37261 actgtcttcc caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat
    37321 gttcaattct tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg
    37381 atgatctggc tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact
    37441 gtacaaccgc ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc
    37501 cataactgac ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat
    37561 aatataaatg ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat
    37621 gattaaaaca aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa
    37681 ccttttaaga ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg
    37741 aagcattagc tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga
    37801 atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga
    37861 aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga
    37921 gccttcgtgt cgataaaatc gatatcagtt ttccagaaaa aatattcaat gcttttgata
    37981 cgaaagaaac atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg
    38041 gtttagctac gatccatgat tttatcggga aagtggatga accacgctat tttatggatc
    38101 ctcgaaggat ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac
    38161 ctaatttttt aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt
    38221 ctgaatttcc tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg
    38281 tggagctagg aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc
    38341 ctgattggta tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca
    38401 aacacttgac gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta
    38461 aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg
    38521 gctgtacttt atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt
    38581 actacagctg atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag
    38641 ggacggctgt ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa
    38701 tgtcgtttat gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga
    38761 acttcttttc cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca
    38821 tctgcaatca ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt
    38881 tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat
    38941 ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca
    39001 gcatgatctg tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc
    39061 atacgttgac gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg
    39121 cttaatactt gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg
    39181 ggctggataa agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga
    39241 agacgaacag cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc
    39301 gttagttgcg ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca
    39361 aaagttttaa gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt
    39421 atttcttctt ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc
    39481 aggctcattt attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc
    39541 gttccatgat agccatttta tggttccagc attcagtaga cagatcgact gggtattttt
    39601 gcggattcaa gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt
    39661 ctttgatttc gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt
    39721 gtaaaactgg gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat
    39781 ggaacatgaa gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc
    39841 cttcagattt cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag
    39901 ttactttttc tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt
    39961 cgatcgatac tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt
    40021 ttgtcccaac accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg
    40081 cttcatccaa gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt
    40141 gttcccgaac acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga
    40201 agttgatttt gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca
    40261 acgtatcata agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa
    40321 atgcttcata atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg
    40381 gtatcccaaa tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg
    40441 ccgctctagt tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca
    40501 atagtggatc ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt
    40561 ggaaattgac catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt
    40621 ccacttggat taatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg
    40681 ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc
    40741 cacgcaagta agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc
    40801 gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt
    40861 caaaaacagc atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt
    40921 gatatagatc cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt
    40981 actcctcttg gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag
    41041 aaatcttcca ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa
    41101 ctccatctgc taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt
    41161 attttattca atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat
    41221 aaaataagta gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat
    41281 ataaccaaat gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct
    41341 ttcctttttt gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt
    41401 ttggaaaggc gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat
    41461 cacacgtttt taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa
    41521 tggcgagaca tcaatcagtc tcgccttgtt ttt
//
LOCUS       DANKHK010000013        39332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000013 DANKHK010000000
VERSION     DANKHK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            34..3300
                     /locus_tag="PW109_000633"
     CDS             34..3300
                     /locus_tag="PW109_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2510507.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEGNAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFRQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPIRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTVKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIDVVVYVMKQRKK
                     "
     gene            complement(3962..5869)
                     /locus_tag="PW109_000634"
     CDS             complement(3962..5869)
                     /locus_tag="PW109_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2510508.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5999..6874
                     /locus_tag="PW109_000635"
     CDS             5999..6874
                     /locus_tag="PW109_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2510509.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEAIELSRWINRKEDIP
                     VLIEEKRSGKELTNCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6954..7928
                     /locus_tag="PW109_000636"
     CDS             6954..7928
                     /locus_tag="PW109_000636"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2510510.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8108..9253
                     /locus_tag="PW109_000637"
     CDS             8108..9253
                     /locus_tag="PW109_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2510511.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPGEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9390..9734
                     /locus_tag="PW109_000638"
     CDS             9390..9734
                     /locus_tag="PW109_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2510512.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10005..11291
                     /gene="efmA"
                     /locus_tag="PW109_000639"
     CDS             10005..11291
                     /gene="efmA"
                     /locus_tag="PW109_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2510513.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVSLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCSTPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11429..12028)
                     /locus_tag="PW109_000640"
     CDS             complement(11429..12028)
                     /locus_tag="PW109_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2510514.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12432..13553
                     /gene="mnmA"
                     /locus_tag="PW109_000641"
     CDS             12432..13553
                     /gene="mnmA"
                     /locus_tag="PW109_000641"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2510515.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLNGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13799..15220
                     /gene="arcD"
                     /locus_tag="PW109_000642"
     CDS             13799..15220
                     /gene="arcD"
                     /locus_tag="PW109_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2510516.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKAWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15234..16352
                     /locus_tag="PW109_000643"
     CDS             15234..16352
                     /locus_tag="PW109_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2510517.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLMILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16412..17704)
                     /locus_tag="PW109_000644"
     CDS             complement(16412..17704)
                     /locus_tag="PW109_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2510518.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17836..18414
                     /locus_tag="PW109_000645"
     CDS             17836..18414
                     /locus_tag="PW109_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2510519.1"
                     /translation="MRGNMEMKKRNLSQEKIINCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMVHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINDFIATLP"
     gene            18543..18920
                     /locus_tag="PW109_000646"
     CDS             18543..18920
                     /locus_tag="PW109_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2510520.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18853..20925
                     /locus_tag="PW109_000647"
     CDS             18853..20925
                     /locus_tag="PW109_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2510521.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLNLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21136..22083)
                     /locus_tag="PW109_000648"
     CDS             complement(21136..22083)
                     /locus_tag="PW109_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2510522.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22329..22793
                     /locus_tag="PW109_000649"
     CDS             22329..22793
                     /locus_tag="PW109_000649"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2510523.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEVKRQLLLLEGYLQN"
     gene            complement(22855..23898)
                     /locus_tag="PW109_000650"
     CDS             complement(22855..23898)
                     /locus_tag="PW109_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2510524.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24275..25240)
                     /locus_tag="PW109_000651"
     CDS             complement(24275..25240)
                     /locus_tag="PW109_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2510525.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     LALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25474..26319
                     /locus_tag="PW109_000652"
     CDS             25474..26319
                     /locus_tag="PW109_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2510526.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26510..27697
                     /locus_tag="PW109_000653"
     CDS             26510..27697
                     /locus_tag="PW109_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2510527.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27703..28059
                     /locus_tag="PW109_000654"
     CDS             27703..28059
                     /locus_tag="PW109_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2510528.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28061..28399
                     /locus_tag="PW109_000655"
     CDS             28061..28399
                     /locus_tag="PW109_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2510529.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28475..28731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28794..29828
                     /locus_tag="PW109_000656"
     CDS             28794..29828
                     /locus_tag="PW109_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2510530.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29821..30507
                     /locus_tag="PW109_000657"
     CDS             29821..30507
                     /locus_tag="PW109_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510531.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30531..31379
                     /locus_tag="PW109_000658"
     CDS             30531..31379
                     /locus_tag="PW109_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2510532.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31576..32349
                     /gene="sufC"
                     /locus_tag="PW109_000659"
     CDS             31576..32349
                     /gene="sufC"
                     /locus_tag="PW109_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2510533.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32362..33648
                     /gene="sufD"
                     /locus_tag="PW109_000660"
     CDS             32362..33648
                     /gene="sufD"
                     /locus_tag="PW109_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2510534.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33645..34880
                     /locus_tag="PW109_000661"
     CDS             33645..34880
                     /locus_tag="PW109_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2510535.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PSIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34867..35337
                     /locus_tag="PW109_000662"
     CDS             34867..35337
                     /locus_tag="PW109_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2510536.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35342..36733
                     /gene="sufB"
                     /locus_tag="PW109_000663"
     CDS             35342..36733
                     /gene="sufB"
                     /locus_tag="PW109_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2510537.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36828..37970)
                     /locus_tag="PW109_000664"
     CDS             complement(36828..37970)
                     /locus_tag="PW109_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2510538.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38009..38743)
                     /locus_tag="PW109_000665"
     CDS             complement(38009..38743)
                     /locus_tag="PW109_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2510539.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38975..39250
                     /locus_tag="PW109_000666"
     CDS             38975..39250
                     /locus_tag="PW109_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510540.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        12752 a         7217 c         8295 g        11068 t
ORIGIN      
        1 aacgctttct aatttgttgc gggaggaata cacttgttaa aaaaacgtat aaaatacagt
       61 gcgctaatga tgaatctcct tatgctcagt accccggttt taatttctgt cgttgaagtt
      121 gctgctaacg aacaacagtc tgtgaacgaa ggaaatgcac acgtgaacat tgaagggatg
      181 tcaacaatgg aggaagtacc aagaactagt gaagatatgg atacggaaga aggaaatgat
      241 cctatgatga aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa
      301 gagtggaaag gggttgtttt cggagaaagc acaagtgcta gtaggtatgc cattgaacca
      361 ttagaagacg gcgtgcggct ctcttccacg aataacggcg gaaaattcca gtcaagcgga
      421 tcagatggaa tgacctatta ttatcaagca atccccaaag acatcaactt taggatgcgg
      481 gcgacgatcg aaatagaatc ttggacctat acgaatgccc aagaaggatt tgctttaatg
      541 gtaagagacg cagtaccaaa aaatcatttg tttaggcagg cattttattc aaattcattt
      601 gccatactag gcagtcggat cgaatatgtc tgggactcgg atagacaaga agtggtcaac
      661 acgagcggca gcaaatatac gatgcgactt ggattaggga cacgggcaat cacaggaata
      721 tcatctgaag atccgcaagc tgcgccagct cctgggagtg tctcagtgga aacaacacct
      781 ttagagactt cagcagggtt cttggaaaaa gaaacaggtt cttataatat attcggaaac
      841 ggtcatggga atctgtttcc ggccacgaac tcgattacta aactggacgt agagatgaaa
      901 aaaacgaata ctggtttaga ggcatattat tacgatcctg aaacaggaga agaaatggct
      961 tctgacatca tgtatgactg ggaaaaatta tacgcttcag atgaatcggt tatttatgca
     1021 ggatttgctg cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt ggcttactca
     1081 gatcctgcga cagatcctcc tggacaagaa aggcccattc gcttgattga tccgatttac
     1141 actgtgacct ctagtaatca ttcaggaaac caagaccata ctatgtcctt tagagcaaat
     1201 gctgacggat tattaacaat caaaaatcaa gcaacaggag aggtacttga gaacgcaaaa
     1261 gacttggcaa tcactgcaaa tcgggaattt gattatcaga caaaactgat ggaaggaacg
     1321 aacgaattta ctttcagttt tacaccagat aaaaactatc cgccagaaga atacgtagag
     1381 atggctagtt atgaaacaaa agagattctg catacagttg actaccgtaa acctaactat
     1441 tcgacagttt atgtaacacc ggaagggtca gatacaggaa acggaagcag acaaaatccg
     1501 ttatctctga cgcaagcatt gagatacgca tatgctgggc agacaatcgt cctggctcct
     1561 ggaacttatt cctttgaaag aggattgacg attccaaaag gagttaacgg gacaagagaa
     1621 aacccgattc aattgattgc agagaaaaat ccagaaaaca agcgacctgt acttgatttt
     1681 aaaggaactg gaaatgggat gacactgttt agtcattact gggttttacg tgggtttgac
     1741 gttacaaata gtgccgctat gcaaaaagga ttgcagattt ctggaaacca taatttgatt
     1801 gaagagatcc atgcttatcg gaatggaaat acaggaatcc aaatcagtgg tagctcaaat
     1861 gatcctttcg aagcatggcc agcgcataac ttagtcaaga actgtacatc ttttgaaaat
     1921 gccgatccag gatttgaaga tgccgatgga tttgcggcca aactgacaat aggagaagga
     1981 aatgtatttg acggttgtat cgcgtatcat aatgcagatg acggttggga cctttttgct
     2041 aaaaatgaaa caggttcgat cggaaaagta atcatcaaaa actcagttgc ttatcgaaac
     2101 ggctgggtac cgggaattga gggagaaggt aacggtaatg gattcaaaat gggcggttct
     2161 tctttaacgg gccctcatga attgatcaat agtttgtctt tcgaaaatct tgcgaaagga
     2221 atcgattcca atagcggaac agatattatc gtcaatagca gtacaagttt taataacggt
     2281 tcatataatg tggctcttta tacctcttca gcaggaaaca cagactacca tgcttctggt
     2341 atcttatctt tccgaacaga gaatcttgag atgagagagc agatccgccc attgaatcaa
     2401 aacgaagatc aaatctacca ctcgttgaac tactattgga atgaacgaga gaaacaatca
     2461 gaaaatactc ttggacatac cgtatcaaaa gattggatcg agtctctaaa tacatgttcg
     2521 aaagaaggtc agcagcctgt tacgcgctat gaaaatggaa gtatccatgt tcatggactg
     2581 atgcagatta aaccggggaa tcgtcaaaca gtaaaggaaa gagtggatgg tcttccatta
     2641 tctgtaggtg ctatttttga cggagaaacc tcgccgtctt cggaatatcc agagtatgca
     2701 atcgtacaag aaccaaatca gacaaaacca aatgaacaaa ataatccgat tattatacaa
     2761 gaagcaaatg gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa gccttttaca
     2821 gtttttgtgt caactttcac accaaatcaa ggaacgcctg tctttgaaga tatgcgttat
     2881 ttcccagaaa agatccaaat cgttgaaaca cagtcaagtg ctcaattttc taatcatacc
     2941 gctatttttt cagcaccttc aaaaggagaa tatacgctag aagtcactta tcgttcggaa
     3001 atatttgatg gggaaaaatg gtctgctcat acagaatctg ttacccagca aaaagcgatc
     3061 actgtgcgag aaactgccga tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca
     3121 ggaaatgaat caccaaatgg aaaaccttct catccggata ggacagcaaa agaaagtgaa
     3181 aaaaatggta ctcaaggact cccaaaaacg ggagaattta ataacccgtt gctagcatta
     3241 gctggaggaa ttttgttaat cgatgtagtt gtttatgtca tgaaacagcg aaaaaaataa
     3301 gtggaatttc tccttttatg atgtaaaaca attgactgag gaaagtaaaa atagaaggaa
     3361 atgagaccaa tagatgtctc acttccttct atctttttat agatgaggtt ctccttgctg
     3421 cttttgattg gctgttctta ttacttgatg caggacagtt ttttcacaac ctctccacta
     3481 aacggtgttc aatcagctga ccctcggtat tagctcaaca aacggcaaaa tatgaggaac
     3541 tatttttccg tttttttctc taatactcag gtcaggacgc tgatttcaca acctctcgat
     3601 aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa
     3661 gccattttca caaaattttt cttactactt gaaactaagc ggctttttca caacctctga
     3721 tatacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3781 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctctg
     3841 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3901 agaagcaatt ttccccaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3961 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     4021 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     4081 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4141 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4201 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4261 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4321 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4381 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4441 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4501 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4561 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaagcga tcttttaatg
     4621 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4681 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4741 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4801 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4861 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4921 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4981 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     5041 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     5101 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5161 ccgtgacaag ttttttcaag tccagtttat ctgtgtattg aataaatgta tcaaaagatt
     5221 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5281 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5341 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5401 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5461 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5521 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5581 attcaacttt ttcgattata ggttgaagtg gttgagcatg caactcattt cttcggtcat
     5641 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5701 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5761 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5821 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5881 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5941 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     6001 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     6061 agataaaaaa attaaaaaat tagccagcac tcagcagttg cagtcaagaa aaagtaaatt
     6121 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6181 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6241 gaagctgatc aatgaagagg caatcgagtt atctagatgg atcaacagaa aagaagatat
     6301 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc acaaattgct gggtgggatt
     6361 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6421 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6481 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6541 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6601 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6661 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6721 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6781 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6841 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6901 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6961 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     7021 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     7081 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7141 aatcaacgaa tgcacctgtc aatgactact atatggaact actgatcatg atcgatgcaa
     7201 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7261 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7321 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7381 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7441 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7501 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7561 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7621 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7681 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7741 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7801 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7861 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7921 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7981 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     8041 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     8101 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8161 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8221 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8281 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8341 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8401 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8461 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8521 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8581 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8641 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8701 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8761 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8821 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8881 tggagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8941 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     9001 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     9061 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9121 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9181 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9241 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9301 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9361 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9421 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9481 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9541 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9601 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9661 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9721 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9781 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9841 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa attttaattg
     9901 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9961 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
    10021 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
    10081 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10141 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10201 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10261 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10321 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10381 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10441 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10501 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10561 ttggtgtcag cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10621 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataacaaag
    10681 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10741 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10801 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10861 catggaaaga ccgtatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10921 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10981 caggagcagg ttgttcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    11041 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    11101 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11161 tgttcttgtt ttctggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11221 ctgctcgaaa ttatgataaa gaattacaga aaaaattggt aaaagaacac catgagcaaa
    11281 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11341 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11401 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11461 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11521 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11581 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11641 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11701 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11761 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11821 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11881 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11941 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    12001 agtgcatatc actggtaaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    12061 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12121 tgtagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12181 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12241 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12301 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12361 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12421 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtaggcatg agcggcggtg
    12481 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12541 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12601 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12661 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12721 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12781 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12841 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12901 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12961 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    13021 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    13081 caaaaaaagg aaatatggtc actcttaatg gtgaagtcaa aggacaacat gctggcttga
    13141 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13201 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13261 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13321 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13381 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13441 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13501 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13561 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13621 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13681 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13741 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13801 ggaaaagaaa tcaacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13861 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13921 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13981 tcatttagga gaaaaacgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    14041 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    14101 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14161 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14221 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14281 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14341 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14401 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14461 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14521 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14581 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14641 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14701 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14761 cggtctgttg ccgaaagctt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14821 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14881 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    14941 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    15001 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    15061 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15121 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15181 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15241 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15301 ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta
    15361 gcgtaagtga atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15421 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15481 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15541 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15601 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15661 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15721 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15781 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15841 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15901 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    15961 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    16021 ctgtacatgc attgaagaat aaagaaaaaa tattggagcg aaacaaaaag atcattacag
    16081 agaaccttat gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16141 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16201 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16261 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16321 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16381 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16441 aagatatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16501 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16561 cacgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16621 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16681 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16741 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16801 caaaacccaa accaatcgca agtattcttt cactcccgat cttgtcacct aagcgaccaa
    16861 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcgatgatt ccgctgatca
    16921 acgtaacatt cccatgtcca tgcagcaatt gacgaatgta cagactgatg attggactaa
    16981 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    17041 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    17101 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17161 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17221 caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt
    17281 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17341 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17401 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17461 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17521 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17581 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17641 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17701 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17761 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17821 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17881 aaaatcatca actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    17941 cagcatttag caaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    18001 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    18061 cgtcgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18121 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt gccccacacc
    18181 aatgaagcag ttttattaga gggaatccat gaaacgaatc agatcatttt gcagatcttt
    18241 gaggcttttc aattaacgaa agaagaaaaa gtccaccgaa gcagagaatt acgtagtatg
    18301 gtccacggct atctttcctt gcgtttttta ggatacttta caaaagaacc gacaatcgat
    18361 ccagaggaca gctatttttg gatgatcaat gactttatcg ctactttgcc ttaaacaatc
    18421 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18481 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18541 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18601 gggaagaaga tattgaagca gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18661 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18721 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18781 tacagcaata caaaggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagcg
    18841 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18901 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    18961 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    19021 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    19081 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19141 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19201 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19261 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19321 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19381 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19441 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19501 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19561 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19621 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19681 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaaa tcttgtacaa
    19741 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19801 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19861 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19921 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    19981 agtaatgaag agcaaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    20041 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    20101 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20161 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcaccatcaa aaaagaacta
    20221 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gacaaatgga aaaatacagt
    20281 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20341 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20401 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20461 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20521 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20581 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatttat
    20641 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20701 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20761 cgttgtaaat acgatggaac gacagaaaaa atgcttgata aaaaagagca gaagaagaaa
    20821 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20881 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    20941 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacggaaa ttgcttctca
    21001 aatttttgtg aaatttcggc ttatttccga agaagctact tctggaacac catttattcg
    21061 gaaaaaagga ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac
    21121 ttcatttgac gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc
    21181 tcttatgata taaacaggac gctgtttcgt ttctaagaag attttcccga tatatttccc
    21241 gatgattcca agacataaca attggattcc accaatgaac aggaagatcg agaccatgga
    21301 aggccatcct gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc
    21361 aataccagaa ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc
    21421 cgagaagttc acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt
    21481 ttcgccagcc acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca
    21541 gctaaataat ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat
    21601 ttgacgagtc atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt
    21661 attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg
    21721 agtcgaacgc cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc
    21781 aggaagcagt tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt
    21841 agaagcttgc aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac
    21901 aaagcggaca taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt
    21961 tgatccgtcg tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc
    22021 atcaaaaaaa agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt
    22081 catcgtttct atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa
    22141 ccttaaataa cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa
    22201 ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat
    22261 tagttggcaa gtagttttgg atagagaaaa aggaggaagt tccagtctat tgaccaggag
    22321 caaacaaaat gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg
    22381 gattgactgc tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcaaattt
    22441 taacgcgcac acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg
    22501 ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga
    22561 ccattcctta tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga
    22621 aagctgtgcg agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt
    22681 gtcatcgagt gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg
    22741 gggttgaagt caaacgccaa ttgttgttgt tagaaggata tctgcaaaat taaaaacagt
    22801 tgtgattcgg acattattcc atgcgagaat cacaactgtt ttttcatgtt cattctaaac
    22861 tttgatcagc caaataaata gtaaagccat cactgtttgg agaagcccga cagctaatcc
    22921 ataagataag aagcgtgtac cttctttcat gaattttgca aagtctaaac gcagaccaat
    22981 tgccgctaaa gcagtgatct caaaccagct gctgataaaa tgtgccgatt ggtcaataac
    23041 tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc acaaacccta atacatacca
    23101 aggaagcggt aattttcgtt ttttctgcgt atcagttggc gtttcaaaca gagctggttg
    23161 tttcttgtgc atgaattttt caaacagata gacaacaaca acgagcatca taatacgtag
    23221 aatcttaaat agcatggcga attcgacaac agaattgtca agtaagcttg ccccagcgac
    23281 tacttgacca acagattgga gtgtaccacc caaaagtgca cttttttcaa tgagatttcc
    23341 accaaacaac tgtaatccaa gaaaagggag agtcaacatc atcacagtac ccagtagatt
    23401 gactagtgtg atgatctgtc ctttttcttc ctctttcgca tggatggaag gggcgattga
    23461 agcaatagca gaagaaccac aaacagcgtt tcctccagcc atcatcaaag aagaagtatc
    23521 gctgaattgc agttttttac caatcaagta agcaccgatg atagtgactg acatttgcag
    23581 taacacaaaa acaattcctt ttataccaat ttgagcaatc gtttgagctg tgacagttgc
    23641 acctagtaaa aaaacagaga attctaatag tttgctttca gcaaattttg tgccgattgc
    23701 tagaacaggc tgtttaaaaa aagtatttcc taataaaata cccaataaaa tagcaatagt
    23761 cgctccacct aactgtggca gataaatagc aagaagcctg ctgaaggcag cgatggcaaa
    23821 agaaagcagt aacccgggta agatttcgcg gaccgctgaa tcctttacag attttattgg
    23881 atgtgacaat gatttcattt gatcggttcc tttcgtcaat aaatactaaa agcatactcg
    23941 aacatagtag cactaaacca gtattttgac aaaagaacaa gaggtcgtga caaaagtctt
    24001 gtcacgacct cttgtttcga ataaacggtg ttcaaaagcc agtttttcgg tattaagcca
    24061 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
    24121 gcggcttttt cacgacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    24181 cccaaccagt acaaaaacat gaacagctgt ttttgtttgg ccgttcttat tacttgatgc
    24241 agaacagctt tttcacaacc tattgaatcg attattaagc gattttttta gaaacaaatt
    24301 ttccatgttt gctatgttct ttaattaaga aacgtacgta atgagcaaga acgtagaaga
    24361 caactacact tgcaaacaga cattccgcaa cttttaagaa atccaatacg ctactataga
    24421 tacctaaact gttgaagtaa tgaacagtag taaatatagc tgttgcagag ataacaagcg
    24481 ggaatgtaaa ggctgcatag cttggataaa aacgaagctt gaacaaacgt ggaagcatag
    24541 acagcacaac gaaatacaat acttgagata aagcgaacaa tgctaagaca agataaaaat
    24601 taggttcagc aaagttttta agataacctg ttaggcaaag agaaccaggt gctgcaacga
    24661 tcgtaatcag cggacgtgtc ggatgagcca tatttcttac gaaaaagaca cgtttgatga
    24721 tgatcggcaa taagaacgca tagcagatca atgaaatcca aaagaaaatt tttccaataa
    24781 gcgggaagaa gttcccggca gttacagtga tgaccccaaa tccaacgtaa atgataaacc
    24841 agcttggata aattgcttca attgaaagat cagctttgat aacatggtga taattgaaat
    24901 aaaagacaag gatcacttgg aagataacag cgcacagcca aatgaatttg acaattggtg
    24961 caatactttc atatgaaaca aaataagtac aaataaccat gagtgacatc gtaaaagttg
    25021 gtgaaacgga ggcgatgatt ggatcatgca acgtttgttt cgtgtgttcg aataagataa
    25081 acaatttaac aaaaatgaat atcataagaa tcatagagat accgccagtt agattgccta
    25141 aagcaacatg gtgatagtct ttaaacagat ttccaaggga agctaaccca aggatcaacc
    25201 cgcagatcgg gatcggaata attttcaaaa agttcttcat atataatatt ctccttttta
    25261 tttaatagat tttagtacca agatgaacga atacattgta aaatgaatag gaatgtatga
    25321 ttacgagcaa gcagacagct gattgtcttc atctaagtta ctagttggat cagcaactgt
    25381 tttgttgtac atttctatta tagatggttt tttgtatatt taaatcaaat agtttttata
    25441 gtaattatca taaaaagtta tggaagtggt gccatgttta agttattaac gacttttcga
    25501 gcagtatatg aaacaagaaa tttttccaga gcagcagaag tactgttttt atctcaacca
    25561 gcagtctcga atcaaataaa acaattggag cgggaattga atatcgatct ttttcaacgt
    25621 aacggaagaa aagagatgct tccaactaag caagccgact tattatatga acggacactg
    25681 gttttgttag aagaatggga agaaactcag caaaaattgc tcaatgaaca aaatgaagaa
    25741 gaagtatgtc ggatcgttgc ttcccacacc tttgctgtct atcttttgcc tcagttgatg
    25801 aaacatctga tcaaaaggtt tcctaaagtg cattttgaga tcgaaatcgc gaattctcat
    25861 gaatcattag aacaagtagc gaaacatgaa tccgattttg gttttatcga aaagccatta
    25921 gagacttccg gcgtacaaag atactctttg atggaagacc agctagtcat cgctggggac
    25981 ccaaaaagca aattgtggct tgtaagagaa gcaaactctg gtgtttttca ttacacgcag
    26041 cgttattttg aagagatgaa tatccaagat aagaagatga tcgtgaaaag taacgatgta
    26101 attgttgcgt tgctgaagga aggaatcggt cgctcgattt tgtctaagcg ctctgttcca
    26161 gaaaatgtgc caatgactcc gttgaatttc catcggtatt tttatttcat ccaacgaacc
    26221 aatctggttt ctgatcagct gctagagatt gccgaagaga tccttcgata caatgaaagc
    26281 acagaaactt ctcacagcga ggagaatctc agctggtaga taattgcatg tatcaaaaaa
    26341 agttctatga tacactaaag ataagcagta ggcttttgta ctgcatttgc tcaagcaact
    26401 gcacaaacgg aaagttaatt tttcccaaat gtctatggaa agactttcca ttatctaact
    26461 ggaccgctat aataatcagc agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa
    26521 attaaaagct tcgaacgata atcgtattga ttatggcgtg attttaccag tctttctgtt
    26581 atgtttgatt ggtctgctct cattatatgt agcacttagc catgatccga acaatccagc
    26641 agtcttgaaa ggcgtaggga tgcaagttgt ctggtattta gttggagcgg cagcaatcgt
    26701 tgtcatcatg catttgaatt ccaaatggat ctggaaactg actccttacc tttatggttt
    26761 ggggttagtg gtaatgggac tcttgctcaa attctatgat cagaatttag ctgcttcaac
    26821 agggtctaaa aactggtttc gattcggtgc gtttactttc cagccagcag agttgatgaa
    26881 gattgcgtac atcttgatga tggcactgat cgtaactaaa cataatacgc aagtaaaaga
    26941 acggacgatg aaatcggact tttggctgat tgggaagctc ttgatcgtga caataccggt
    27001 tttagcattg atcatggccc aagatgactt tggaacgatg cttgtctttc ttgcgatctt
    27061 tggcgggatt ttcctgatgt ctggaatctc ttggcgcatc atcgcacctg tagtaatctt
    27121 ggcagtggtc gttggagcag gaacgatctt cttggttacg actgaaggcg gaagagactt
    27181 gctttacaaa gttggcttca aatcttatca gtttgcacgt attgattcat ggctggaccc
    27241 tttccatgat acgtcaggaa tgagttatca gccggcccaa ggacttttgg caattggtac
    27301 cggtggcttg tttggtaaag gattcaacgt aagcaatatt tatgtgcctg ttcgtgagtc
    27361 cgatatgatt tttaccgtta tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat
    27421 tttcttgtat tttattttaa tctatcgtat gattcgggtg tgtttcgata cgaataatga
    27481 attttacgcc tatatcgcaa gcggtttgat catgatgctg ctgttccatg tctttgagaa
    27541 tattggagca aatatcggat tacttccttt aacagggatc ccattgcctt tcattagcca
    27601 aggaggatct tcgattttag ggaacatgat cggtatcggg ttgatattat cgatgagata
    27661 ccaaaatgaa gcacctgcaa gatcttcaag gaggtaagaa aattgtacac cttttactgg
    27721 tacccgaaat gttcaacatg caaaaaagcc aaagcgtggt tagaccagca tggcgttagt
    27781 tatcaaactg ttgatatgat caaagaagta ccgaaggcct caacgattga gacttggcta
    27841 gaaacgagtc aagtaccaat ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa
    27901 cttggactga aagatcaagt aggcgagttt acggcttcaa aagcgagcca agtcttatcg
    27961 acagatggta tgctgatcaa gcggccactg atcttacgag aggaccagct tgttgcaatt
    28021 ggattcaatg aaaaaattta tgaaggagaa ctgatataaa atggcaggca agcatttgaa
    28081 gaaaaaggac aacctttgga tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa
    28141 tgaagcgcaa gaagaacttg gagacatcac gtttgccaat ctgccaaaag caggacaatc
    28201 gctaaaaaaa gatgaacctc tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc
    28261 cagcccgcta acgggtacag tttcttctgt taatgataaa atcggtgaag atatcaatat
    28321 cttgaatgat tcagatgaaa tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa
    28381 agaatttgac gaattataaa atcagaaaat cacttgaaaa aagttgacac tgattcttcc
    28441 agttgctata atcataagca atcctatatg aaacgagaaa gctttgaaaa gaagagtaca
    28501 tgtcacggac gtttgaagag agccctgttt gctgaaaatg ggtaacgaaa atgagatgga
    28561 agatggtctt agagcagagt aagcgagcgt aagcaaactt actacgggag tgcccgttac
    28621 agcgcgacag tatacggaaa gaagctaagt ccaagtactg gaagaggctg ttattgcgaa
    28681 gtaacggtaa atcaaggtgg taacacggaa gttcaagctt tcgtcctttt gcttaaggca
    28741 agagggcgaa ggcttttttg tttgaaaaat cataagagaa agatgtgaat ggcatgtcat
    28801 taatccaact aacgaatgtc aaaaagacat tcaatgggaa aaacggaaac atccaagccg
    28861 tcaatgacgt ttcactaaac gttgaaaaag gggatattta tggaatcgtc ggctattctg
    28921 gcgcaggaaa aagtacatta gtccgtctcc tgaatggact tgagcttccc acgagcggcg
    28981 aagtgatcgt aaacgatcaa gatattacaa aattaaaaaa caaagaatta cgtacattcc
    29041 gaaagaaaat cggtatgatc ttccagcatt tcaacctgtt atggtcaaga actgtacttg
    29101 aaaatatcca gctgccgctg gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg
    29161 cagaagaact gttgcgttta gtcgggttag aaggacgcgg acaagcatat ccttcacaac
    29221 tttccggcgg acagaaacaa cgcgtcggta tcgcacgagc attggcaaat gatcctgaga
    29281 tcctgctttg tgatgaggct actagcgcat tggacccgca aacaacagaa gaagtattag
    29341 acttattgtt agcgatcaat aagaaattga atttgaccat cgtcttgatc acacatgaaa
    29401 tgaatgtgat ccgcaaaatc tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag
    29461 aagaaggaga cgtattgact gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg
    29521 tacaacaaga catcgaacca gaagctgatt cgacagaact attagctgaa ctcatcaaag
    29581 aaaatccaag cggccgtttg gcaactttga cctttagcga aagaaatgcc aacgagccag
    29641 tcatttccca agcgattcga cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca
    29701 aacaagtgaa agaaggttcc tttggttcat tgactgtctt gatgactggt gaaaccaaag
    29761 aattagatca agcagaagtg ttcatcaaag aacaaggagt aggaattgag gtgatccatc
    29821 gtggataaaa gcttttcaga aacatatcta gattttaaac aaatcaaccc ggaaacgatg
    29881 aatcaggcta tccttgatac actatatatg acattaatct caatggtctt agtgacgatc
    29941 atcggattag ttttgggatt gatcctttac tctattggac gcaaaaacaa accgtatgct
    30001 cgaatcattt atggaatcgt atcgatcatc agtaatattt tccggtcaac gccatttatg
    30061 atcctgatgg ttttgatcat tccatttact aaagcaatcg taggcactat gctaggtgcg
    30121 caagcagcca ttccagctct tgttttatcc gcagcaccat tttatgcccg tctagtagag
    30181 attgcttttc gtgaagtcag ccctggtgta ctggaagcag cagatgcaat gggagcaagt
    30241 tactgggaaa tcatctggaa ggtcttgata cctgaaagta ttcccgcatt gatttctgga
    30301 ttaacagtta caacgatctc gatgatcgga tttactgcga tggccggcgc gatcggcgca
    30361 ggtggacttg gaggtcttgc ttggcaggaa ggctaccagc gtggtaatct gacagtgaca
    30421 ttcgttgcga ctttaattat tttactaatc gtatttatcg ttcaaggaat cggtgatttc
    30481 ttaactaaga aaacagacaa aagataatag gatatagggg gatgtaggat atgaaaaaga
    30541 aaatttttgg atttgcagca gcgcttttgc tgactgtagg attagcagca tgcggtaaca
    30601 acagcgacag caaagattca gcaaataaag cagacgacac gacattgaaa gtcggcgctt
    30661 caccgacacc gcacgcagag attttggaac atgtgaaacc attattgaaa gatgaaggaa
    30721 tcgatcttga aatcgtcaaa ttcgatgact atgtattacc gaaccaatct ttggaagaag
    30781 gcgacattga tgccaattac ttccagcata ttccttattt gaacaaagaa atcaaagaaa
    30841 aaggatatga ctttgtcaat gccggtgctg tccacatcga accaatgggc ttgtactcta
    30901 aaaaagtcaa agatgtttcc gaattaaaag atggtgcaac aatcattact tcaaattctg
    30961 aatcagactg gggacgcatc atcacaattc tacaagatgc tggcttagtc aaagtcaaag
    31021 atggagtaga tctggaaaca gcaacattcg atgatattga agaaaaccct aagaacttga
    31081 agttcaacca tacgatcgac ccatcacttt tagcttctac gtaccaaaat gatgaaggtg
    31141 acctagttgc aatcaacgca aacttcgctt acggcgcagg gttgaatcca ttagaagatg
    31201 ccgtattgct tgaaaaggac aactctcctt acgtgaatat tattgcgact cgtaaaggtg
    31261 atgaagacag tgacaaaatc aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag
    31321 aatggatcac agacaaatgg ggcggctctg ttaaaccagt aaaggctgat gcaaaataag
    31381 gtatggaaca aaaaagatga gaaataagtc tgtaatggct tattcctcat cttttttata
    31441 cctattctaa tttagaaatc aaaattgttt aataataaat aaaagatatt tgattttagt
    31501 aaggaaggtc attgcgcttc attctcaatt agtctaaaat gagtgagaac gaaagagaat
    31561 ttgggggtat aatgcatgtc tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa
    31621 aaagaaatcc ttaaaggcgt aaacctaaca atgaaaaccg gagaaatcca tgccatcatg
    31681 ggacctaacg gcacaggaaa atctacctta tctgccgcta tcatgggcaa tccaaactat
    31741 gaagtaacag aaggcgaaat tttgtttgac gggaaaaatg ttttagactt ggaagtagat
    31801 gaacgtgcgc gacttggctt atttttagct atgcaatatc caagtgagat accaggtatc
    31861 acgaatgcgg aattcatgcg tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt
    31921 tctgttatgg catttttgaa aaagttagat gaaaaaatgg ctttactaaa tatgccagaa
    31981 gaaatggctg aacgttatct gaacgaaggt ttttctggcg gagaaaagaa acggaatgag
    32041 atcctgcaat tgttgatgtt agaaccaaca tttgcaattt tagacgaaat cgattcaggt
    32101 ctggatatcg atgctttgaa agtcgttgct aaaggagtaa atgaaatgcg cggagacaat
    32161 ttcggtgcat tgatcatcac ccactatcaa cgattattga actatatcac acctgatgtc
    32221 gttcacatca tgatggaagg ccgagtagtc atgacaggga atgctgacct agctaaacgt
    32281 ctggaagcag aaggctacgc tggtatcagt aaagaattag gaatcgacta taaagaagaa
    32341 gaagcgtaag ggggagcaag aatgaaagaa aaaaactggc tcgattatct tgacgcggta
    32401 aatgattttt ctttaagtaa aggcgaacct gattggatga gaaccttccg tcaagacgca
    32461 ctagcgaaag cagatgaact gccattgccg catatcgatc gggtgaaatt ccatcgctgg
    32521 tcgttatttg atgtgaaaga aacacaaacc atcagtgaaa caggcacgat tccggcattt
    32581 gatgcaatga aagacaatcc tgtgcttgtt caacaaggtt catggacgat ttttgaacaa
    32641 ctgcctgtgg aattagcaga aaaaggagtc atttttaccg acttgttcac tgcaatgatc
    32701 gaatatccag aactagtcca agaatattat atgaaaaaag cagtaaacat gaacgaagac
    32761 caattgactg cactccatgt agcttttatg aacagcggta ttttcctgta tgtgcctaaa
    32821 aatgtcgtga tcgatgaacc actcgagtcc ttatttattc aagatggcgc atccgatgag
    32881 cacttcttca agcatgtgtt gatcgtggca gatgaacata gtgaattttc ttatctggaa
    32941 cgtttccaga caaccaaaga acaagtagca aaatcatcag gtaatatcat cgtggaagtc
    33001 atcgctaaag ctggttcaaa aatcaaatat tcagccgtag atcaattagg agaaaatatt
    33061 acttcttata tgaatcgccg tgggcacatt ttacgagatg cttctgtcga ctgggcaatt
    33121 ggtgtcatga atgacggtca tgtgatcgct gactttgact cagatttagc aggagaaggt
    33181 gcacacgcag aagtcaaaat cgtggcaatc agctcgggca gacaaatcca aggaatcgat
    33241 acgcgagtaa ccaataaagc ccctcatacg atcgggcata ttttgcaaca cggagtgatc
    33301 cgagaaaaag gaacattgac ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt
    33361 gcagatgccc aacaagaaag tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg
    33421 aatcctattc ttttgatcga tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga
    33481 cgagttgatc cagaagaaat gtactatctg atgagtagag gattgcataa agaagaagca
    33541 gagcgcttag tgattcgagg attcctcggt tctgtcttaa cagcaatccc tgtagaacaa
    33601 gttcggaaag aattagtcga agtaatcgaa gggaagttga atggatgatc gatgctaaaa
    33661 agctacgcaa tgattttcct atcttatttc aagacgtcaa tgatgaacct ttagtttatc
    33721 tggataatgc agcgacgacc caaaagccaa aacaagtact ccaggcatta gcgcactatt
    33781 acgaacacga taacgccaat gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc
    33841 atgattatga acaagcaaga gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag
    33901 aagtgctgtt tactcgtggt acgacgacaa gcttgaattg ggtagccaga agcttcggcg
    33961 accgctatat caaagcagga gacgagatag tgctctctta catggaacat cattctaatg
    34021 ttatcccgtg gcagcagttg gcaaaacgaa caggtgctgt cttgaaatat attgaactaa
    34081 cagaagaagg cttcctggat atggaggatg cagcgaaaaa gattacggac aaaaccaaaa
    34141 tcgtctctat tgctcatgta tccaatgtct taggtgtcat caatcctgtt caagaattag
    34201 ctgaacttgt ccatgaaaac ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc
    34261 acatggccat tgatgtccaa gcactagact gtgatttctt tgctttcagc ggacacaaaa
    34321 tgtgcggacc aacaggtatt ggtgttttat atggtaaacg aaaatggtta gaagaaatgg
    34381 aacctgttga atttggtggc gaaatgatcg attttgtcga attatacgac agtacatgga
    34441 aagaactacc ttggaaattc gaagcaggaa caccaagtat cgccggcgct atcgcattag
    34501 gccatgcaat cgattatttg caaaaactcg gtatggacaa tatccatcga tatgaagaag
    34561 aactagccgc ttatgtgttg ccaaaattac aagcaattga tggattgact gtttatggtc
    34621 cacaagaccc gaattttcgg acaggagtcg ttgcattcaa tttggatggg ttacatcccc
    34681 atgatgtcgc gacagcttta gacatggaag gggtagccgt cagagcaggt catcattgtg
    34741 cgcaaccgct attgaagtac cttgaagtat cagcgactgc tcgcgcgagc ttttatttct
    34801 acaacacaac gcaagacgcc gaccgtttag ttgaagcaat catggcaaca aaggagtttt
    34861 tccaacatgg gactatctaa attagacaat ttataccgtc aagtgatctt ggatcattct
    34921 tcccatccgc atcatcgtgg aagcttgact gactcgacaa gcaagatcga attgaacaac
    34981 cccacatgtg gagatgtgat cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat
    35041 atcgcctttg acggcagtgg ctgctccatc agtacggcaa gtgcctcgat gatgacagac
    35101 gccgtcatcg gaaaatcaat agaagaagca gaacatttaa ctgtagactt tctacagctt
    35161 gtccaaggaa aagaagtcaa agatcccgac caattaggcg atgctgctat gcttggtggt
    35221 gtagctaaat ttccagctcg gatcaaatgt gcgacacttg cctggaaagc actaggaaaa
    35281 gcaatggaaa acgatggtac cgcagctttc catcattacc acaaggaaga ggagtgagaa
    35341 catgggcgta ccagaattag aagaatataa attcgggttt cacgatgatg tcgaaccggt
    35401 tttcagtaca ggagaaggct tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg
    35461 cgaaccagaa tggatgcttg aattccgtct gaaatctcta gaaacattta acaaaatgcc
    35521 aatgcaaaca tggggccccg acctttcaga catcgatttc gatgccatca aatattacca
    35581 aaaaccaagt gacaaacccg ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac
    35641 atttgaaaag attggtatcc cagaagcaga acgagcttat ctagccggtg cttccgcaca
    35701 atatgaatca gaagtcgtgt accataatat gaaagatgaa ttcgaaaagt taggaatcgt
    35761 ctttactgac actgactctg ctttgaaaga ataccctgaa ttattcaaag agtacttttc
    35821 aaaacttgta ccgccaactg acaacaaatt agcagcgctg aattcagccg tatggtcagg
    35881 tggcaccttt atttacgtgc caaaaggcgt caaagtcgat gttccattac aaacttactt
    35941 ccggatcaat gcggaaaaca cgggacaatt cgaacgtacg ctgatcatcg ttgacgaagg
    36001 agcaagcatc cactatgtcg aaggttgtac ggcacctact tattcaagta atagtctgca
    36061 tgccgccatc gtagaaatct ttacaagaaa agatgcgtat tgccgttaca caacgatcca
    36121 aaactggtca gacaatgtgt acaacttagt aaccaaacga gcaaaagcat acgaaggcgc
    36181 aaccgttgaa tggattgacg gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt
    36241 ttacttagat ggaaaaggcg cacgaggcac catgctctcc atcgcctttg caggagcaaa
    36301 ccaaatccaa gatacgggtg caaaaatgat ccacaatgca ccaaatacat ctagctctat
    36361 cgtctctaaa tcaatctcaa aagacggcgg agaagtaaac tatcgaggac aagtaacctt
    36421 cgccaaaaac agtgcgggtt cgatctctca tatcgaatgt gacacaatca tcatggacga
    36481 atggtcgaaa tccgatacga tcccatttaa tgagatccac aacagccaag tctctttaga
    36541 acacgaagcc aaagtctcaa aaatctcaga agaacaactc tactatctga tgagccgagg
    36601 cctgaccgaa tctgaagcaa ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa
    36661 agaacttcca atggaatatg cagtcgaact caaccgattg atcagttatg agatggaagg
    36721 cagtgttggg taaacctgtt ataggagcta gaacccttga tatataaggg ttctagcttt
    36781 tttgttttgc ctgtatacta gaaatgacca gattttgacc agctctttta ctgatgagcc
    36841 atcatatcag caaagtcttg acctgttttc ttaggtgcat tatttgtaat atgtgcatag
    36901 atgtctaatg tcactttact attttcatgc cctaatcgtt gttgaactac cttgacagat
    36961 tctcctgctt ctaataataa actagcatgt gtatgtctaa acccatgagg tgtaatacga
    37021 ggcaagttat attttttcaa aatatatctt aaccaatcat taacagtgtt tggaatgtat
    37081 agagtgtttt taactgttgt aaaaacatgt tgctctttag agctagtatt gtatccaagt
    37141 tttaaatact cttctttttg ctgtaatttc cagttgttga ggatagaaag agtctcagta
    37201 tctaaagaga taactcgtct agaagctctt gttttcggag attgaattat aactttacca
    37261 aattcatcta cagcaagagt tttattgaca tgtagttctt tattaaacgt gtctatgtct
    37321 ttccactgta aagcgagtac ttcactcttt ctcatacctg tgtatgccaa taatctgaag
    37381 aaggcgtatt gtttcatgtt accaaaatct ttaaaagcat ccagtaaatt attaagctcc
    37441 tcttttgagt aaaatttacg atttttctca tactcttttt ttcgtggaag aagtgttttt
    37501 tccataggat tattgtcaat atattgcatt gcaaccccgt agcgtaaaat ttgtgcagtc
    37561 tgttttctca tataatcgta actttcatat ttactgtgcc attcatctac cactttttga
    37621 caatatggga cagagatatt cgatagttta agcttaccaa atgctggaag tatttgatta
    37681 tttgcgtatc ttactgagat tgcgattgtt gaagctttaa cactggtacg ctgatgagtt
    37741 atccattcat tatacaggtc ttcaaatgtc atatcaggtg ctttcttttt aacaccgtat
    37801 aatatttgat gtctgtactc gtcataatct ttttgagctt cttgtttgct actaaaacct
    37861 cttctcgtag tatttatttt ttcaccagtt cgtggatcgg tggcaatgtg tcctttatac
    37921 atccacgcca cttgaccgtt tttctttttg tactttgata tttttgccat taaatcatct
    37981 cctcttagtt tacaaggagt acttaccact agatggtatc acctcccttc atttttttct
    38041 tagtgtattt tttcccaatt gatgaaatcg tttgttctag cttgtttata ttatcagtaa
    38101 tttctctcac agcttcttcg ggataagaca tgtctttcaa ttgaataggt agccattcat
    38161 ctaccattct ttctagtagt tcttctatac tgctagtgag gaaaagaatg ttattgtcaa
    38221 attcttctac aatagcattg atgaataatt cttctatttt taagatattg aaaagactaa
    38281 ttttttcatt tttaacttta ttacaagtct tgttagcaat aaattcttgc atctctggtt
    38341 ctaatttgga atatctatcc agccatgtat ctaaattaga gtgtactggt gggaactgtt
    38401 gacgaagatg ctggaacagg tttttatctg gaaatgttat accatctagt acaatgctat
    38461 ctctataaag aataattccc aaaatatatt cttctacatt gccacaaact aactcctcta
    38521 tactcatatt tcccaaatca gcaattcggg ataatcgctc tggtattggt aaataggttc
    38581 ccttctccca ttcgtagata gtttttttgt caattaattg accgttgtta atcataagac
    38641 caaatgctgg ttgtgttaat tttttactct ttcttatagc ttttattttt gttccgagta
    38701 tcttttttcg ttctaaataa tttattggtg gacgttttat catattgacc acacctccta
    38761 aaattagtat acctcaaaaa aggggtgttg taaataaatt ttacaaagct gttgacaccc
    38821 cttttttatc gaggtattat gtgtatgtcg atgaactggt aatcattttt tgtttgatta
    38881 atcggttaat aacaaaatac ttttattttg agcaaaaaat acgaagataa ttcttagaaa
    38941 atttgttcaa gcaatcacga aaggaggggg aaatatgtct ctacaaatca ttgatagcat
    39001 tgtttctata caagttgaac aacaagtgaa agagataatt acagtgctaa agcctatgct
    39061 agaagacatt atttctggag tcactctaga gtatatggac tatcagcagg caagtgaata
    39121 tctaggtgta tcagtaggaa cgattagaaa atatgtttct caatacgggt tacctgttat
    39181 aaaaattgat acggtcatta gatttaaaaa atctgatatt gatagtttca tggaacggta
    39241 taaaggttga gtaaacaaat aagactaaaa catatagtaa taaagagttt gtttttatta
    39301 tctgtatgtt ttagtcttta acagaatttt at
//
LOCUS       DANKHK010000014        39134 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000014 DANKHK010000000
VERSION     DANKHK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            1..294
                     /locus_tag="PW109_000667"
                     /pseudo
     CDS             1..294
                     /locus_tag="PW109_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface adhesion protein"
     gene            321..503
                     /locus_tag="PW109_000668"
                     /pseudo
     CDS             321..503
                     /locus_tag="PW109_000668"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface adhesion protein"
     gene            525..1265
                     /locus_tag="PW109_000669"
     CDS             525..1265
                     /locus_tag="PW109_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2510541.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1689..1859)
                     /locus_tag="PW109_000670"
     CDS             complement(1689..1859)
                     /locus_tag="PW109_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2510542.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(2053..2232)
                     /locus_tag="PW109_000671"
     CDS             complement(2053..2232)
                     /locus_tag="PW109_000671"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510543.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2619..3731
                     /locus_tag="PW109_000672"
     CDS             2619..3731
                     /locus_tag="PW109_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2510544.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3809..4102)
                     /locus_tag="PW109_000673"
     CDS             complement(3809..4102)
                     /locus_tag="PW109_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2510545.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            4270..4584
                     /locus_tag="PW109_000674"
     CDS             4270..4584
                     /locus_tag="PW109_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2510546.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4605..6584
                     /locus_tag="PW109_000675"
     CDS             4605..6584
                     /locus_tag="PW109_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2510547.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6597..6923
                     /locus_tag="PW109_000676"
     CDS             6597..6923
                     /locus_tag="PW109_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2510548.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6939..7457
                     /locus_tag="PW109_000677"
     CDS             6939..7457
                     /locus_tag="PW109_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510549.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7457..8809
                     /gene="celB"
                     /locus_tag="PW109_000678"
     CDS             7457..8809
                     /gene="celB"
                     /locus_tag="PW109_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2510550.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8893..10398)
                     /locus_tag="PW109_000679"
     CDS             complement(8893..10398)
                     /locus_tag="PW109_000679"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2510551.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10490..11080)
                     /locus_tag="PW109_000680"
     CDS             complement(10490..11080)
                     /locus_tag="PW109_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2510552.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11259..12905
                     /locus_tag="PW109_000681"
     CDS             11259..12905
                     /locus_tag="PW109_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2510553.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12954..13673
                     /locus_tag="PW109_000682"
     CDS             12954..13673
                     /locus_tag="PW109_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2510554.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            14152..15099
                     /locus_tag="PW109_000683"
     CDS             14152..15099
                     /locus_tag="PW109_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510555.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            15111..16031
                     /locus_tag="PW109_000684"
     CDS             15111..16031
                     /locus_tag="PW109_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2510556.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            16072..17529
                     /locus_tag="PW109_000685"
     CDS             16072..17529
                     /locus_tag="PW109_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2510557.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17874..18488
                     /locus_tag="PW109_000686"
     CDS             17874..18488
                     /locus_tag="PW109_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2510558.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18481..20205
                     /locus_tag="PW109_000687"
     CDS             18481..20205
                     /locus_tag="PW109_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2510559.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            20202..21653
                     /locus_tag="PW109_000688"
     CDS             20202..21653
                     /locus_tag="PW109_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2510560.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(22126..24279)
                     /locus_tag="PW109_000689"
     CDS             complement(22126..24279)
                     /locus_tag="PW109_000689"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2510561.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(24281..25327)
                     /locus_tag="PW109_000690"
     CDS             complement(24281..25327)
                     /locus_tag="PW109_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2510562.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25477..26766
                     /locus_tag="PW109_000691"
     CDS             25477..26766
                     /locus_tag="PW109_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2510563.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26779..29481
                     /locus_tag="PW109_000692"
     CDS             26779..29481
                     /locus_tag="PW109_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2510564.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29466..30323
                     /locus_tag="PW109_000693"
     CDS             29466..30323
                     /locus_tag="PW109_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2510565.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30394..31293)
                     /locus_tag="PW109_000694"
     CDS             complement(30394..31293)
                     /locus_tag="PW109_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2510566.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31462..33633
                     /locus_tag="PW109_000695"
     CDS             31462..33633
                     /locus_tag="PW109_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2510567.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGKVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33701..34042
                     /locus_tag="PW109_000696"
     CDS             33701..34042
                     /locus_tag="PW109_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2510568.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(34318..36072)
                     /locus_tag="PW109_000697"
     CDS             complement(34318..36072)
                     /locus_tag="PW109_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510569.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(36083..36919)
                     /locus_tag="PW109_000698"
     CDS             complement(36083..36919)
                     /locus_tag="PW109_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2510570.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36921..37604)
                     /locus_tag="PW109_000699"
     CDS             complement(36921..37604)
                     /locus_tag="PW109_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2510571.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(37763..>39134)
                     /gene="uxaC"
                     /locus_tag="PW109_000700"
     CDS             complement(37763..>39134)
                     /gene="uxaC"
                     /locus_tag="PW109_000700"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2510572.1"
                     /translation="LKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNNFSNLTEAW
                     LGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWSHLELKRFF
                     GIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADDLHYHQLLA
                     HEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVLGQRIEYFH
                     QHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETLIHLTHFYH
                     SHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAEETDQLPKT
                     ILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQFADGSALGN
                     FVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQAISYTNAKN
                     YFGFFA"
BASE COUNT        12570 a         6931 c         8031 g        11602 t
ORIGIN      
        1 gtcgtgttga caaagatcga tgaccaaagc ggggaaatcc tgcaaggagc tgtttttgag
       61 ttacagaacc gagaaggaga aacgctacaa accggtctaa ctacaggaga agatggaaaa
      121 ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca agcgccaata
      181 gggtatgaat tagatgcgac accaatcgag tttgagatcg aaagaagtca gacagcggtg
      241 gtagaattaa cgaaagaaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat
      301 gaccaaagcg gggaaatcct gcaggagctg tttttgagtt acagaaccga gaaggagaaa
      361 cgctacaaac cggtctaact acaggagaag atggaaaact ggcaattgac ggtttagcac
      421 caggagcata tcagctggta gaaactcaag cgccaacggg gtatgaatta gatgcgacac
      481 cgatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagcaaacc
      541 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcggg gaagtcctgc
      601 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
      661 caggagcaga tggaaaactg gcgattgacg gcttagcacc aggagtgtat cagctggtag
      721 aaacccaagc gccaacgggg tatgaattgg atgcgacacc aatcgagttt gagatcgaaa
      781 gaagtcagac agcggtggta gaattgacga aagaaaatcg acttacacca ggcggtgtcg
      841 tgttgacaaa gatcgatgac caaagcggag aagtcctgca aggagctgtt tttgagttac
      901 aagatgccaa tggaaccgtt ctgcagtcag gtctaaccac aggagcagat ggaaaactgg
      961 cgatagatgg cttagcacca ggagcatatc agctggtaga aactcaagcg ccaacggggt
     1021 atgaattgga tgcgacacca gtaactttca agatcgaaaa agaacaagaa gtggcgatct
     1081 ttttaacaaa agagaataga aaagttgctg atagctcaga tataagagga aacaatccaa
     1141 caagcagtgg aaataagcat cttccaaaaa ctggagaaac catcattagc cagtttatat
     1201 tgtcaatatt gggtgtgcta cttgttttca tcagtataaa atttagaaag aaacgaaata
     1261 tatgagctaa aataaaaaat tgaataagca tagtttttaa aatcaactag tacccatcaa
     1321 ttgaatggaa actaccgatt ttagaagggg tatacaaaca aatattagta tcggagtaga
     1381 gaaggttgaa cccaaccagt aaataagtaa agacaatttt gtgctgcgtt gagaagtgaa
     1441 aaataaacct ttgtagtaat actagttaat aaaaaatata caaaaacact gcgaaaatca
     1501 ttcttgagtt tcgcagtgtt ttttgcctaa tgactcaagt ttattgggta atactttgat
     1561 tttctttcta caaataatca gcgatcagtt gtaatttttt ctctagtttt aatggctctt
     1621 tgtcaataag gagaaaaaca agtcgggtct tttgtctcgg aaagcttcaa ccaactcgtg
     1681 aataaaggtt acgcctcctt gaaaggcgtg gaaaaaccaa gtaatcgatc aatcaccaat
     1741 ttcgcatttg gtagaacatg tttggtgagc tgaaagtagg tggcgttcat gtctgtaact
     1801 aagaattcta tttctttagg attagtgcag ctaaggaaat agtttgttaa tcgaggaatt
     1861 ttttgtgtag gtaaattttc tcgattaatt tttctgtctc gccatccgcg gaaataaaac
     1921 tcagtttatc ctcaacggaa acgtgagaac gaaattcaca accctcaaca ctcaagacag
     1981 aattcttttg gaagactttg gtaaatagct tctcaactct ttcaatgtgc gaataacggt
     2041 tgtcaaagat acctaacaac ttttcgaaat aaaaaataac gacacttttt cagtcagtaa
     2101 agaagtgatt ttatgtctga catgatttga aatcgagtgc cttggacgga taaaataact
     2161 ttgagcgttc caatggcttc gacagttttt acaggtatag cgctgctttt ttaggcgtat
     2221 aaccattggc atatgattat attattcaaa gtggacaatg gtttccttct tcttgttttt
     2281 caccaccatt aatttcctat ttccatctac cacagcagaa ccacaatttc tacagacaca
     2341 tggagcaggg aagagaactg cagcaacgat caatgttttt ttcttctgta aggtttcgta
     2401 agagacctct gtaatcatca aatttttgtc tattgatctc agaatttttt tgatagaacc
     2461 attcataaag catatcgttc tctcagttgt ttattttgtg gtgatttaat catacaagaa
     2521 aacgatatgt ttttttataa aaacatgaat aggaaaaata gttgacaaga aaatgacaac
     2581 gccatatgat gttccatagt atttacaacc tgtcatagat gactgtaaaa aataaaagta
     2641 aaaagaaagg acgacaacag gtggattttt atcagtctct acaactagat ccgtttattt
     2701 taaaacaaaa aatacgagaa gcagcttcta aaaaagaaaa gtgcatgtat atttgttcat
     2761 tgtttttacg atcattattc atcgtattgt ttgccatttg ttttatcatt ataattacaa
     2821 cattatttga atcgaagcat aaaccatacg ctgtggtttt attctgtatg ttgatgagta
     2881 ttcgttttgt tgactttggt tataagattt cccattcgat cataggttta gcaatcgtca
     2941 tgttctcact attagtagca ccgtatgtcc agctgatcaa atggtcagtt atgggcatgt
     3001 tgattcattt tattttacta agcagcatat taatggcaac cgctagtgat cccaaaatgg
     3061 ggaatgcttc tttgtacggc ttttcatatc tctttattgt ttattcgtta cctaaagatt
     3121 cgttgaacaa gaatttcttt acacaaacaa gtagcttatt atttttgttt ttttgttggt
     3181 tttcagttct cctttacaga aaacatcgag aaaagaacaa agacaaaagc ttgtttaaag
     3241 agattttttt gaaaggcatg tattcgcagg aaaaaatttg gatgctcatc tatgcatttg
     3301 gcattagtct tttgatcgtt gcaggagagt atgtcccttt tcagcgattg atgtgggcag
     3361 ggtttgcatt ttcttctatt gtatctagct atggattgat gtcgattggt ttcaaagaac
     3421 gagcagtaga ccgaatcatt ggctcattga ttggatgtgt tttatttatt ggtatcagcc
     3481 aatttattcc ttttaactgg gtcggtatat taggtggtct agctttaggt gtttgttcta
     3541 cctatagata taaaacagtt tttaattctt ttggtgcttt ggcaatcact gccagtttat
     3601 tcggggttcc aggagcagta acgatccgca tttttgagaa tatccttggg gtatgtttag
     3661 gaatcatgta tattggggtg actgaaatac ttatccgaaa aataagagaa aaacacggtt
     3721 tgaatcatta gatgagatcg aataaaaaat aaggaaaaag tgaagataca catggatgat
     3781 aaattgctat cattccatgt gtatcttttt acggtaaatc agtgaaacgt tcgactttca
     3841 tttttaattt atacttttta cggagttcgt ttatttgagc catcatttcg gatttgtgat
     3901 gcttttcgat tgcttcttca tctttccaac ggtcgatcag taaaagagat tctggatctt
     3961 ctagtggaaa gtaatagtca tatttctcgt ttccttcttc tgcacgaatc gctttgacaa
     4021 tgtttctttc gaccatttct tctgcaaatt ttctggcttg tccatttgta cttgtataca
     4081 aaatattcac tgtgatactc actaaatccc ctcctgaaaa aattctatca ctagtatatc
     4141 acggtgccag aaattattat tcaagatatg atacacattt tttccgcaat gctacggaaa
     4201 tatatatttt gaatagaaaa cgcttacaag atatgcttgg tttataaaaa taaaaggagt
     4261 gattgattta tgaaaaaagc attgattatt tgtgcggcag gaatgtcatc ctcgatgatg
     4321 gcctcaaaga caacggattt ctttaagcaa aaaagagaag aggtactcgt agatgcagtt
     4381 tcagctacag aaggcgataa tatgattaag aatagtgatt ttgatttatt tctgatcagc
     4441 ccgcaaacga cgatgttttt ggataaattc aaaaaattag gagaatctgt tggaaagcct
     4501 gttgttagta tccctttcca agcgtatgtc ccaatcccta caggcatcca gaagttagca
     4561 gaggtgatcg aagagaacat atgatggaat aggagtggat gataatgaaa cagaaggaaa
     4621 aattgctgtt gagatatttg attgatcaca agaaggagta tgtcaccagt caacgtttag
     4681 cttctgaatt gttattatct gatcggacaa tccgtaatta cttgcagcgg atcaaagagt
     4741 tagtagaaaa aaacggaggt aagatcattg ctaaaccagg ttatggctac caactgcata
     4801 ttttacaaag actgacattt gacttgtttt tgagtcggca agaaatagta cctggatatg
     4861 ctagagaacc gcaaggattt tatgagtccg aagatcgaca aaagtatata ttgaaccaat
     4921 tgcttttgga agatgccatt ctttttatgg atgatttggc agaagagctt tatatcagtc
     4981 gttccagttt gacaaaggac atgcaagaaa tcaaggagcg attgattcca tattcactaa
     5041 aaatcgtttc taagcatggt caagggacat ggatcgaagg ggaagaaaga aatcgacggc
     5101 attttatcat ggatactttt tttggaaacc attatggaaa ttcattgaaa gaatatttag
     5161 ggcaaagccg ttttttccca gacatcagtt ttgaagagct agtcattatc attctcgatg
     5221 aaacaagaga agctaagtta aaagtttcgg attttatcat acagaatctg actttacact
     5281 tggctttggg gatcaaacgg ctccgagaag ggtttgaaat caaggaatta ggcatcgaac
     5341 aagaagtttc taagcgagta gagtatcaag tggcgcagcg gatcgttcaa cgtattgagg
     5401 taatcgcagg tgtatcgttt cccgaagaag aaattgctta tttgacatta catctcatgg
     5461 ctaagtcgaa tcacaaagaa aatcaaaatg atcaggaact atcaaaggaa ttagcgaaag
     5521 ttctgaaaag attgtctcag gaatttcatt tttcattagt agaggatctc cagcttttca
     5581 atggactact cgatcatttg tcaccgatga tgattcgatt gagccgaggt attcgcattg
     5641 agaacccatt gactgaggag ataaagaaag aaaatcccaa agtctttgac gcagtaaaaa
     5701 ggcatttcag caacatgccg gctttgaaaa actataccat caatgaagat gaatgggcct
     5761 atttggcttt gcatctcatg gcggcattgg aaaaagaaag agctgcgcat aaactacatg
     5821 cattgattat ctgtgcaacg ggttacggca gtgcccaact attaaagaat cgcgtagtca
     5881 gtgaattcgg taaaaatatt acagtggtaa gtgtaaaagg ctattatgag atcaatgaaa
     5941 caactttaaa aggaatcgac ctgattattt cttctattga tctttcgaca atgtttttca
     6001 aaattccagt tttacatgtc agtatttttt tgaatgaaga tgatgttcgt aaaatacgaa
     6061 aggtagttgg agaatctata ccctatcatt cctcagatag accgatacca gcattttctc
     6121 tacagcaaaa aaagcagatt tacaccgaac agttgtctga aaaatttttc aagacttata
     6181 cgtacgaacc gaaaaaggaa gaaattcttc atgatttgct gcaaaagctg agcataaatg
     6241 aagaagaagg atatataaga gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac
     6301 aaatcatatt cagtgataca gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa
     6361 caaaggtagc cgttgcattg attccttctg ggatgcagtg ggatcaatat caagaaattc
     6421 attttgtttt tttagtttcg ccttcttatg tcgaaaatga aggaattacg gtcgtgacta
     6481 aagcaatcgt taggttagtt gaccagctaa aagtacagca ggaaatctta gctgaaccta
     6541 tttttgagaa tttcagcagt aaatttatca aactgatcga ataaggagga aaacagatgg
     6601 ctgaaaaaat aaacagtgaa acactacaag tgacttcttt tgatatcatt ttccatagcg
     6661 gaaatgcacg gacactgata catgaagcat ttcgctcgat gcgtaaagag atgtttgaag
     6721 aagtagaaga gaagttgcga caagcaaatg aagagattct tcatgcgcat cagtcacaaa
     6781 ctcaacttct gaaagaatat gccagcggga caaaaataga aatggaaatt atcatggtcc
     6841 atgcacaaga tcatctgatg acgacgatga cattattgga agttgcacag gaaatgaatt
     6901 atttgtatca aaaaaataaa tagaaaggaa ggatcattgt gcaggataca gtaaaaagga
     6961 aaatcgaatt agaaaaaaag caacttagtt tgaaaaacat gtatttcaat cgttatctaa
     7021 tcgtacgtta cctgactgcc tttttctttt tcatgaacat gcaatggatg attctgcttc
     7081 tatcagctaa aagtttagga agtagtttac cgatgattct gattttagct attttgcctg
     7141 cagtaggaga acaagtaaag ctttatcgaa aacaccaaac gaacgttcca tggacaaagt
     7201 ggtatttttt gttccagagt gtctttaatt tattagtgat tccagtgttg tttacctcag
     7261 gatttacatt cttgtatcct tttatggcaa ataacaatgg aggacagtta tttgttttca
     7321 ttcttgttgt aagcggtatt tttgccagtg catttataca gtatcgttta aagaaaattt
     7381 cactcaacca agaccaacag tatatccgta ttaaacaata tgaaaaagcg ttatatttag
     7441 gaaaggagaa taactaatgt ttgatttttt gcagaaatac ctgatgggac ctatggggaa
     7501 aattgcgcaa cttaaaatcg ttcgggctgt catggcagcg gggatggctt ctattccttt
     7561 cacgattgta ggctcgatgt ttttggtttt aaatattttg ccattaccgt tcccattttt
     7621 agaaggattt tttaatgcaa cttttttcaa agttagtgac ttgtacatga tcgtcaatac
     7681 aatgacgatg gggattttat ctatttactt tgccattgtg tttgcttatg aattaacgtc
     7741 aattgaacga gatgaacaaa aattaaatgt taatccgctc acgggggcat tactatctgt
     7801 ttttgcattc tttatgtgta tccctgaatt gatcatgact gatggtaaaa tcagcttgat
     7861 tgcttctatc aatgagacag aaactgtagt aaatggtatt aggatgggct cttttgtcga
     7921 acgtttagga acttcaggaa tctttacagc tattattatg tcgattattg cggtcgaatt
     7981 gtattgtatg tgtgtcaaga gaaattgggt cataaaaatg ccagatgttg ttccgccagg
     8041 agtttctcgt tctttcactg ctctgatccc aacatttgtc attgcgtttg tggtgatgat
     8101 catcaatgga ttattagttg cactaggaac ggatatcttc aaaatgattg ctatcccatt
     8161 tgggtttgtt acgcagttga cgaatacatg gcttggtgtc atggtgattt atttcttgat
     8221 ccatgcattg tggatcgtag ggatccatgg agcgaatatc atcacttctt tcttgacccc
     8281 gatcgtatta gctaatatgg cagctaacgc acaaggcgca aattatccgt tggcaggaga
     8341 attcaacaat tcttatgtaa ccgttggtgg atcaggtgcg actttaggat tgatcatctt
     8401 tattgcattc ctcgcgaaat ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc
     8461 aggattattc aatatcaatg aaccaatcat tttcgggatg ccaatcgtct acaatcccta
     8521 tttagcggtt ccgtttttct tagctccgat ggcttctgct acactcgctt attttgccat
     8581 tcggctggaa atcgtcaaac cgatgcttgc acagatgccg tggccttcac cagtcgggat
     8641 cggagcattt gttggtagtg gtggtgattg gaaagctgct gtgttagcag tcatttgtgc
     8701 aatcatcgca ttcattatct ggttcccatt tatcaaattc tatgataata aattattaaa
     8761 tgaagaaaga gaaaaggcag cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa
     8821 aaaggaactg tgacaaaaaa tcacagtccc tttttttctt agaaatggga aaccactagt
     8881 atataaatac cattattctt caatcaaccg cttcacgatt gcataatccc cttcataagg
     8941 ttcatctaca ccattgattt tttgataggg atccacgcct tttcccgcaa taacgactga
     9001 atcttgcgga gcgctttctg ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata
     9061 atgaacgatc aaatctggat tagtaattgc ttcattgatt tcttccgcga tttttttcgg
     9121 atcttcaaat gctgggtcat ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac
     9181 ttttccaaaa tcatgtcgac gggagattgc tttgtttcct gggctgccta atacaacaat
     9241 tacacggcca tctggatgtt catttttggc aaattccagc aatgttttca agcttagata
     9301 attatgtgca tagtccacat agacatgcgc tccattggat tggatcaatt gatccattcg
     9361 gcctggtaca cgagcttctt ttaatccgtc ttgtgcatct tgactggagg cacctgttag
     9421 tgctgcggca atgatcgcac tggctgcatt ttcttggttg aatcctccag ccagcaaaat
     9481 atcataagca gtatcgcaga tagctaagcg atcttcatga gaagataaag taaaaccgtg
     9541 agtaccttta tctgaacgga tcacttgata attgctttct tccgaacgac cataagtgat
     9601 cgttggaatc tggaatagat cggctgtttc cctaagtaaa tcaaaatagt cactttcatg
     9661 atttaaaaca actgtttttg aattcaagat caattgtcgc ttgcaataga aatagtcatc
     9721 aaatgtcgga tgttcgatcg gactgatatg gtcaggcgag atattcaaga agattccgac
     9781 atctaaagtc aagccgtata cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac
     9841 tagatgagtc ataccatttt caactgcttc tgacatcata cgatataaat ctaatgactc
     9901 aggcgttgtc aaatgggatt tgaaatatgt tttgccatct aacgttgtat tcatagtaga
     9961 aagcaatgct gtttttttat ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc
    10021 agttgtttta ccttttgttc cagtgaatcc gatcactttc aatttatttt gcggatagtc
    10081 gtaaaaagcc atagcaagaa gcgccattgc tttacggata tccgttacga tgattcctac
    10141 tgctgaattt tcgtatggtt gttctgatac ataatattga ataccctgtt cgattgctga
    10201 atccaaatat tcttctttga aattcattcc tttacagaaa aacaaagtag aagcatcagc
    10261 ttttcttgaa tcataactta actgaagaaa ttcagctttt tctggtacat caagatgcca
    10321 gcctcttgtt gaaacaaatt cttttaatag attttctttt aatagtaaat gacggatgtc
    10381 ttctaaagag aagctcattg attgatccct cgctttattc aatttcttgg tttattatag
    10441 cacactcttt ttaatatatc gtttgtttcc gctatgacaa aatccagctt tattcaaatt
    10501 ttcttgacac aggggacaag cggatgagta ttttcataaa caatgaactg actgtaatag
    10561 ataggagaac gaatagtgtc tccaacaatc caaaaacata ataattcaaa aagacgataa
    10621 gactattggt aagtaaaagt ccttgcggca ttggaagccg aaaaaatttg tggaaaataa
    10681 tcggcggtat cgttataccg ccatttgagg ccccgactgt gtacaatgtg taaaccccaa
    10741 gactgaacaa aagacttcct gaaatcagtg aaaagaaatg tgaggagatc atttccactt
    10801 ttggaacaat ctctaaaaca acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata
    10861 ctgattttcc taataggaca gcggctagaa gcagcatcat aagattgatg ctccaaacag
    10921 taactgagcg ttggatagaa aaggccgttt caactaaatg tccgatggcg cccactccgg
    10981 cggaagctat atcgtgaggg agcaagaacc aattcaatcc aactgtgata aagaaaagac
    11041 ctaaaaatat aacgggtagt ttttttattt cttgtgtcat tgatatctcc tataaatgtt
    11101 agccataaaa ggctgtatat ttctttactt taccaaaaaa acgaaaccga attcaaaaaa
    11161 acaattgttc ttataaaagt tatataaaat cgattgccag tttctgtttt accaattaaa
    11221 atgatagtaa tgttttgtag aaagttggaa tctgaccgat ggataatcaa agaaaaccag
    11281 cattagatca attaacaccg caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca
    11341 gtaatatcat ttcaagattg ctaggcgctg tctatatcat tccctggtat gcttggatgg
    11401 gcgaaaatgc taaggctgcc aatggattat tcaatatggg atacaatata tatgcattgt
    11461 ttctactgat ctctacagca ggaatacctg ctgcgattgc aaaacaaact gcacgctata
    11521 attcattgaa tgaatatggc acttcacgac gattatttat ccgtgcatta cagatgatgg
    11581 gcgggctagg cctgttgttt gctttgttca tgtacatcgc ctctccttgg ctagctcatg
    11641 cttctggtgg cggagaagaa ctgattccaa cgatgcgttc tttgagtatc gctgtattgg
    11701 tttttccttg tatgagtgtg atacgagggt attttcaagg caatcaagaa atgatgcctt
    11761 atgcgctgtc gcagattgta gaacaggtgg cacgtatttt ttatatgcta ttggctacat
    11821 tcatcatcat gaaagtcatg gatggtgact atgtgacggc ggtcacgcaa tcaactttcg
    11881 cggcatttat cggtatgcta gcaagtattc ttgtcctttt gtattttttg aagaaacatc
    11941 aagcgtatac atcggcgttt attcactaca gcgaaaacaa aataaatatt gcaacgaagg
    12001 aattgttgtt agataccgtt aaagaagcga ttccgtttat tattgtgggt tcaggggtaa
    12061 cgatcttcaa attagtcgat cagttcactt ttatgaagat catgtctgca tccaccgagt
    12121 attccaatgc acagttactg gatctttttt cgatttttag tgccaatcca gataagttga
    12181 cgatggtagt tattgcattg gcaacatcga tcgcgtctac tgggttaccg ttgattacag
    12241 aagctgttac gttgaaagat cgagtaggtc tggcaaaatt gacaagcgga aatttacagt
    12301 tgttttcatt ttttatgttt ccggcgacat ttggtgttat gttacttgcc tatccgctaa
    12361 atacattatt ttatacaccg gattctctag gaagtaatgt actgatccaa gcaagttttg
    12421 taggactctt cctaggacta tatatgctag tttccaacat gctgcaagga atgtttgaaa
    12481 acaaggcggc aattaattat ttggttattg ggtttctagt gaaactcctt cttcaatacc
    12541 cagccattcg tttatttgaa gtatacggcc ctttattggc gacaatgatc ggatttgctg
    12601 tgtcttgtac cttgatctta aagagaatcc atgaagtagc ccgctttaaa ccaagttttg
    12661 tctggcgcag gacattgctg atctttattc ttaccttgat tatgcttttg gcttcgtgga
    12721 tcacaaagat gatttttggt gcttttttaa acgaagatag caaattccag tctctattgt
    12781 tgattcttgt agtagcaggt ataggggctt tagtgtatgg ttatttagct ttgaaaatcc
    12841 gcttggcaga aagactacta ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa
    12901 aataagtgaa tcagaagagc tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt
    12961 tagataagtt tttagcagat atgggacttg gcagccgcaa agaagtcaaa ggattgatca
    13021 aaaaaggaac gatcgaagtc aatggacaag tggtgaaaag cgataaagcg caagtaaatg
    13081 aattcgaaga tgcggttact tatttaggag aaatcattac gtatcaaaaa gatttttact
    13141 acattttgca taaacctgca ggagtcattt ctgctactca ggataatcat gatcaaaccg
    13201 taatcgattt attggaagac caggattatc gggaagatct ttttccagtc ggccgattag
    13261 ataaagacac agagggcctc ttgattttaa cgaatgacgg ggtgtttgcc catcagttac
    13321 tatcacctaa aaaacatgtg gagaaagaat atctagctga ggttagcggt ataatgacag
    13381 aagaagatat ccgtttattt gcagaaggac tgattattga tggcgatgag cagacattgc
    13441 cggcaaaact ccaaattgat caagtagatc aagagacaga aacttcaatt atccgtctga
    13501 ttttgcatga aggaaagttt caccaagtta agcgaatggt caaagcagta ggaaaagaag
    13561 tcttatattt aaaaagaatc cgtatgggaa aattttggct gcctaaagat ttgaataaag
    13621 ggcaatatcg acccatgacc aaagaagagc tagaacaagt gagagaaaaa taattgaatg
    13681 gatagctaaa agaataagca gagtagttgt agcgccatct gaaaagcaga cttgtccgtc
    13741 tgactttcgg atggcttttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga
    13801 acaccgtttg ttcggaataa gggactgtga caagactttt gttacagtcc ccattgttcc
    13861 ttgtgactca gagttagata gtaaggacac gatctcccta atactcaggc caaaacgctg
    13921 aattcacaac ctctgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    13981 cggcaaaata tgaggaacta ttttcccgtt tttttctcta atactcaggc caaaacgctg
    14041 atttcacaac ctctcctata ctttgtttca ttttccctat actttgttta tttattctca
    14101 ttattgaaag cgctataatt acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa
    14161 cgaaaggggt tcaatcattt ttttatgaat ctatggcgtt ataaagcact tgtcctgatg
    14221 gctgtaccgg gtatgctttg gatgattttt ttcttttaca tacctgtaat ggcgaatgtc
    14281 gtagcattca aaaattttca tatttcagca gatgggttct tggctagctt gagggaaagt
    14341 ccttgggtgg gttgggaaaa tttcaaattt ttattttctt cggatcaagc atttctcatc
    14401 actaaaaata caattttata taatgtagca tttatccttt tgaatctgct gatctcagtc
    14461 gtatttgcaa tcattatgag cgagttgaga aataaacgac tcgttaaagt ttatcaaacg
    14521 atgtccttac taccttattt tctatcttgg gtcattatca gttactttgt ttacgctttt
    14581 ttaagtcctg acaaaggaat attcaatcag tggatcactg gacatggagg agaagctgtc
    14641 aattggtata atgaaccgaa atattggccg tttatcttgg tgttcattgg tacatggaaa
    14701 gggattggtt ataacagcat tatttacttt gcatctgtta tgggaatcga tccgacttat
    14761 tatgaagctg ctatggttga tggagctagc aaatggcagc agatcaaaca tgtgacgata
    14821 ccacaactta ttccactaat gacgatttta acgattttag ccgtaggaaa cattttccga
    14881 gctgattttg gcctgtttta taacattcct aggaattccg gtgcattata tgatgtgact
    14941 caagttttgg atacgtatat ctacaatggc ctgacatcca caggagattt tggtatgacg
    15001 gctgctgcag gactttacca atcagttgtc ggctttgtct tgttgatgat cacaaatacg
    15061 atcgcccgac gttttgatag cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa
    15121 agaaagtgga gagagtccaa gtccgctctt tcaataagac ggctaatttt ttctctaata
    15181 taatgattgc actctttgct atttcctgta ttttgccatt tatatttgtt atcgccatct
    15241 cttttaccga tgagtccata ttgtcgactc acgggtacag tttttggcca gaaaagttca
    15301 gtatatttgg ttatacgtac ttgttcgacc agatgcagga taaaattttc caagcgcttt
    15361 tcgtcactgt acttgttact gttttaggta cactgatcaa tagcacggct acttctttat
    15421 atgcttatgc gatttcacgg tcaaactttc cattccgtcg tttttttacc gttttttgtt
    15481 tgattacgat gttgttctca ccagggatgg tggccaatta tcttgttatg acaaatcttc
    15541 ttcagttaaa agatacgatt tgggcattga tcttgccaat ggctgtcagt cccttcaata
    15601 ttattgtgat gcgcacattc tttaaacgtt cggtctctga ttcgattatt gaatcggcac
    15661 ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat cgttttaccg ttagctgttc
    15721 caggtattgc gacaatcagt ttatttgccg cattgggtta ctggaatgac tggttcaatg
    15781 ctttactata catccaagat gataagctag ttccactaca gtatcttttg atgaaaatcc
    15841 aaagtaatat tcaatatctg acacaaaatt caggagcagg aagccaattg acaggcggat
    15901 tagcctcagt tccaggagaa tctgccagaa tggcgatcgt agtcatctcg acgttgccta
    15961 ttgcgatcag ctatccattt ttccagaagc attttgtcaa aggtctaaca atcggcggag
    16021 taaaagaata aagaaacaaa aatataaatc ataaaattag gaggaagaaa aatgaagtta
    16081 tggaagaaac tagcctttac aggtgtttca gcgttgttgt tgggaacatt agcagcatgc
    16141 ggaggttctg gttctaaaga ccaggcagaa gctagcaatt cggacacttt gcagatgtat
    16201 caaatcggag ataaaccaga taatttcgat caattgatgg aagtagcaaa caaacgaatc
    16261 gaaaaagaga tcggtgtaaa agtcaatatc aattacattg gctggggcga ttacgaaaag
    16321 aaaatgaacg tcatcatttc ttccggggaa aattatgata tcgcctttgc gaacaactat
    16381 gtttccaatg cccaaaaagg agcctttgct gatttgactg aattagcgcc taagtacgct
    16441 gaaaaagctt ataatgattt agatgaagca tatattaaag gaaatctggt caatggaaaa
    16501 ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac aagtacttac ttttaacaaa
    16561 gctttactag ataaatacga tctttcaatt gatggaatcc aatcttacgc tgatgcagaa
    16621 aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa
    16681 ggcttcaaag tccaagggaa ctttgattat cctttaggga atacgttacc atttgctatt
    16741 gatctggatg gagatgattc gaaaatcatc aaccaatacg ataatgaaac gtatatcgga
    16801 ctgttacgta caatgcatag ctggtacaaa caaggactca ttccaagtga cgctgcaacg
    16861 agcaataagg attatccact agaaggaaat acttggctaa tgcgtggaga aacacaagga
    16921 ccgtatgatt atggcgatac aattttgaca aatgcagcaa aacaagaact tgtctcaaaa
    16981 gcaattactg ttccactaaa atctacagct caagcacaaa tggcaaattt tgtcgtatca
    17041 aatacttcaa aaaataaaga aaaatcagta gaacttctag ggctgcttaa cagtgatcca
    17101 gaattattga atgggcttgt ttgggggatc gagggagaag catgggaaaa agtcgatggt
    17161 tcagatgata aaatcaaatt gctagatggt tatcagccaa atacacatat gtctgcttgg
    17221 aatacaggaa acaacaagat tctatataca caagagtcta ttacggatga catgattgcc
    17281 aaacgtgatc aatcgattgc tgatgcagaa acttcaccga ttttaggatt cagcttcaac
    17341 actgacagtg tcaaaacaga attaagtaat atttctaatg tcatgaatca atatttagat
    17401 ggattgaata cagggactgt tgatccagat gaaacattac cgaaattgaa agacgctttg
    17461 gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa aacaatatga tgagttccga
    17521 caagaataat aatgaataaa gacaaagggg ttgtgacaaa agacgtgtca caacctcttg
    17581 ttccgaataa acggtgttca aaagccagtt tctcggtatt aagctaaata tatgtaaaaa
    17641 tgtggaaagc catattcaca aaatttttct tactactcga aactaagcgg ctatttcaca
    17701 aactcttggt aaacggtgtt caatcagctg accctcggta ttcgctcaca aaacgacaaa
    17761 atatgaggaa ctatttttcc gtttgtttct ctaataatca ggtcaaggtg ctgattgccc
    17821 aacctttttt tgtataatga gaaagtataa acttaatgtt tggaggagaa gatatgttag
    17881 ggaaggtttt ggaagagtta tttatccgga tctgggtcat tataaaacta accttgtatt
    17941 tctggatata tacatttgcc ggcgggatca tctttggatt aggtgcggca tggaaaacag
    18001 tcaatgaact attttatttg tacggattcg agtataaaga aatcacaata aaaagaggct
    18061 ggaatatata taaaagaaat tttttaagag gcaatctgct atttagttta tttttatctg
    18121 gtactgcttt attgagttac aatttatatt tatctgttca aatcaaaggg ctcatttttt
    18181 tagctattga ttttatttta ctttttgcct tgctctgcct atttgctact tatcaataca
    18241 gtatgatttt agatagtgaa tatacgatct ctgttcctaa tctgctaaag ctaagtttta
    18301 tttcggtttt ttccagcttt tcgtcttttc taaaaataat tattggtagc ggtataattt
    18361 taggcgtgac ttggcaattc aaaggattga tcctgtttgg tgtcattggt cttttgacgg
    18421 tttggaatgg tacgatgaca acacattggc gtgaagaatt agataaacag ttggaaagtt
    18481 atgaataagt ttcggattct attttcaaaa ggaaccttac taaatcgtct gttgcggaaa
    18541 tacgcggtta tcatgatcgg tgtaacgatg actgctaccg ttatttttag tatccatact
    18601 tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg atgccgttca aagcacaagc
    18661 agaatgctga atgataaaac aaccttgagt aggatcatca aaaaccagct tgtagggaat
    18721 tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac ccatagatca gtatctgatg
    18781 tatgtatacg aacagcaaaa cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa
    18841 gatctctacg caaattatga agaactaagt gcgatatata tcgtgttgaa tcaactaccg
    18901 gagtattatg aatcttctag agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa
    18961 ttactagacg ccttttatat caagttgcct atcacacaag gaggagctga gtcaggaagt
    19021 atttttattg gttttcaaaa gaaagaacta gatatgattt tagaaaatct aacgtcattc
    19081 aatggactct ctttatacat gatttctgga acgacaaatc gactatacac attccatgat
    19141 aataaaatag ctaaagaaac gtttgaaaga caagaaatga tcatcacaga gagtctgaag
    19201 aaaaattcac aattgccgat tgacgatctg gaaaaaaaca attttgttca acaccaagaa
    19261 ctgtctgatg attatcgtat attagctgta ctagataaaa aaagtgtaaa aatcgaaacg
    19321 ggaaaaaatc ttgccccgct gatcattggt atcattattt tagacaatat actgcttatc
    19381 tttctttata aaacattcaa acgatattca cagcaagtat ctatcgtcat gcaggccatg
    19441 gatcaaacag cagcagggaa tttagagacg cggatcgaca taacaaacac ggaatatgaa
    19501 ctgaaggaac tttcaatcgg tatcaacgaa atgttggata gcatcaatca gtttgtagaa
    19561 gacatctaca aattagaaat caaacagcaa gacgcacata tgcgggcgct tcaagcacag
    19621 atcaatccac actttttata taatacatta gaatatatcc gaatgtatgc aatcagcgaa
    19681 ggaagtgaag aattagcaga tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg
    19741 aatcaagaga aaacgatcac tttaaaagaa gaactggact tttgcgaaaa atatgtctat
    19801 ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt ttatgatcga tcctgatttg
    19861 gaaaagatcg aagtacctaa atttgtgatc cagccattag ttgaaaatta ttttaaacac
    19921 ggtattgatt ttactcgatt cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa
    19981 cgggtaagga tcatcatcaa agataatgga aaagggatga ctgaaaaacg gctaaaacag
    20041 attgaagaaa aactgtctca tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac
    20101 gtgaatgaac gtttacgtgc cagcttcggc agcagttatt atatgtcgct tgagaataat
    20161 gagacaggtg gactgaccgt ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt
    20221 tattggtaga tgatgaatac atgattttga atgggttaaa aaagataatt gattggcaaa
    20281 gtttaggctt tcaaatagta gcaacagcag agaatgccaa agaaggacta gctgtacttg
    20341 aacagcgaca gattgattta gtagtaacgg atgtgacaat gccagaaata aatggactgg
    20401 aatttattga agcggctcaa aaagaacgtc ataattttga atttatgatt ttatcaggat
    20461 accaagaatt cgattatttg aaaggaggta tgcaattagg cgccgtgaat tatttgatga
    20521 aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc
    20581 agcaaaatga acaaaaaaat caacaagaaa tctatcaaga actgttattc agtcaatggt
    20641 tgaatgaaga attggatgaa ataagtgaag aagaaataat aggacaaata agtgagaagc
    20701 agcataggat agtattgatt caactggcaa gaactcatga tactcttatc aatagatggt
    20761 tgcaagaacg gcaacaaatc ttttactatc aacgaaatta cggagaccgt gtattgatca
    20821 cgttgttgtt ggaggcagat gaagttgatg gcttctgttg ttttgttcaa gaaaactttc
    20881 ctgatcagga atggctgatc agtatcgggg aagagacaga ttctttggac aaaatccctg
    20941 aaacattcca acaagcgaaa gacaatctac agttgcatca attttatgga gataaagacg
    21001 agcatgtttt ttacgcagta caagcagaaa caaaagatca aaccatcgat ttttcaagtt
    21061 tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca aaaaatgatc actgattttt
    21121 ttgaacagtt tcagttagca gccatgatgc cggaagatat acgatattcc agttttttac
    21181 tgtttatgga gattcaacgg gaattgattg atctggaaga tgaggaatat ttacaaggaa
    21241 tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca gcagctactt ctgtcattca
    21301 tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc
    21361 tgcaccagca ttatcaagaa ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa
    21421 atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt
    21481 tgaaccattt acgtatggaa aaagcgaaac aactgctttt gcacagtaat caaaacatca
    21541 atgagatagc aagtgagatt ggctacaaca acaccacgta tttctctaaa ttattcaaga
    21601 aaatcgtcgg cagatcccct aaagagtacc gagaaaacag tggagaatca taaaaaaaaa
    21661 agccaatgta atgaaataac ttataaaaaa ttgtctagaa atctaatttt gggctgttct
    21721 tattacttga ggcaaggcag ctttttcatg acctctcgat aaacggagtt caaaagccag
    21781 tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    21841 cttaatactc gaaactaaac agctttttca cgacctctcc actaaatggt gttcaatcag
    21901 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc
    21961 tctctaatac tcaggtcaga acgctgattt cacaacctct caataaacgg tgttcaaaag
    22021 cttgcatctg cggtaataag accaaccaaa cgaaaaatat gaggatctat ttttgcttgg
    22081 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttgttaat tatagtttga
    22141 taaggaaaaa ggcaaatctt ttgcttgata tttcttaggg tgagggatga tccctaattg
    22201 gtaagagatc gtgccgcctt tcagcaaatc ttgatgggta aaatataatt tcgtatattc
    22261 tctgtccatc cgtttgattt tatggataaa ttgttgctgc ggccgattag gcgttgcttc
    22321 aatcgtcaat tcctttccat ttgacaaaag caaaacagcc ttgtcaatta atggcatacc
    22381 gataacatat tcaccggttc caggcgtgac aggataaaat cccagactat tgaagatata
    22441 ccaagcggcc atacttccat tgtcctcatc tccagggtaa cccattggag aatcgtcgaa
    22501 ggtctgagtc atcaactgtt taagcagtgg ctgtgccatt tcaggttgtc ctatataact
    22561 gaacaagtaa ggatagtgaa aacttggctg gtttgaaata gctaattgtc caaattctat
    22621 cgcggccatc tcactcattt cgtggatctc aaaaccatat cctcctgggt caaacgttgg
    22681 gggctgattg cacagctcaa tgagtttttc tttatatctc tcactgccac catattttcc
    22741 gatcagtcct gaaaaatcgt gataaacagc aaagctgctt tgccaagcac ttccttctgc
    22801 ataatcttgt ccccaacgat tagctgaaaa tggttctctg aacttgccat ctttatcttt
    22861 tgaacgcata aatcctgact catagtcaaa gatattttga tagttcaatg attgctgctg
    22921 atatttcttc gtcatttcct cttcttctag taccttggca acttggctaa tacaaaaatc
    22981 gctataagca tagtctaatg tgtgattgac tgattcgtgg tagtgattag gaacatagcc
    23041 atatgtcaag taatcttctg ttccttggcg tccataattt ggatgtttgc tttgaaccgt
    23101 agcacctttt tccattgccg ttagaaattc aggcatcaag tctcgtcgga tatcttttac
    23161 agcagcatct gcaatgaccg catcgatcaa tgtcccaggc ataagtccac gttcatcagg
    23221 agacagccat tttggcaaat agccactttc tctatagctg ttcaaaaaac cttccagcat
    23281 ttcttcgtat ttttctacag caatcaaaga ataaagagga taaaccgttt tatacgtatc
    23341 ccaaaaccca ttatttgtat agagaacacc aggttttata gatcgactag tcgtatcata
    23401 gtggattttt tgcccactag gatcaatttc ataaaatgtt tgtggaaata aaaacacccg
    23461 atataaatta tgataaaacg tccgcaattg ttgttgatca tggtgtgtga gctgtatacg
    23521 atcaaaatag gactgccatt gttcttgact gtgagcaagg taagtctctt tgctttggtt
    23581 ttgttctagt tccaggttat aagctgcttg ttccgagctg ataaaagatg tcccaaatcg
    23641 gatagtttgt ttttttgtct gttcaaaata caacgggatc gagtcgctat ttttgttgga
    23701 aacagctaac tcatcaattg ttaaaggata ttcaaatctc aatataaaat aaaaagaaaa
    23761 atcaggatct tctgaaccag caaaatgtgt gatttttcct tccactgtgt aaggatctat
    23821 aacaaagagc tgatggcgtc ccggaaagga taaaagtaaa ccgctgtcat ttttgctgta
    23881 atcaatagat aagattccgc catacatgct aggtatcaat gtagaacaga tgccataacg
    23941 taattgacag atcgacagat gagtcgggca aaatatactt tcttctgggc ggtaagaact
    24001 ttgagcatgg aaaagggtgt tctcctgtag ttttccattg atcggcgtaa acagcatatg
    24061 actgaaatca cccatccaag gacttggttg atgggtaaga cggtagcctt gaaaaacgcg
    24121 gtcatcagga tgaaaccacc agctgccgtt ttgatccgta gtttgcggtg caaaataatt
    24181 catcccaaaa ggaacgccag tataaggctg gcagttccca ttagagaaat ttggctgatt
    24241 cgctgttgca tgtctagtat ctatttgacc gatattcatt ttatttctcc tttgttgatt
    24301 gtcttttgat gaatgaaact ggaaatttac tatcggatgg tcgatctttt gtctctaacc
    24361 aagtgaccaa agcatctccc gcaagcttac ctaactgttc aaaatcttgt gcgatggttg
    24421 tcagtggcgg gtcaacaaga gctgctgctt ggatatcatc aaatccgata attgaaaaat
    24481 cttcagggat atgataacca tgctgtctca agagattcat ggtctggata gcgacaacat
    24541 cgttttcaca aacaaaagca gttacttcat acttagtgat ataagaaagc aattcatcta
    24601 atgtcgcgct tttgtcatcc agtgaagtgt ggaaagtcaa accagctttg tccaatgctt
    24661 caaggtaacc aaggtaacgc tgtcgaacgg attgtggatg agtctgcgta ccaaacaaat
    24721 aagcgatttt tgaatgtccc tgttcgatca aataagaggc agcaagcgca ccgccttgga
    24781 aattatcgga aagaacggta ggaaaaggta actcatagat ttttttatct aatatgacga
    24841 cagggaattc tttcaatgaa agctcaaaaa gtaagtcaag atgttgttct gtatcaagag
    24901 cataataaat caatccgtca aattcattga ttagctcttt tgctgtttta ttttgtagaa
    24961 agtcaagcgt cgtcatcatg acatcaatct gtttatcttg catcacaggg ttcaatccct
    25021 tgctaaaatc accaacagac aaatcagcga catatggaag aagaaacaga atccgtcgtg
    25081 tttttgtagg agaagcaaga atctgttccg gagaagcgga tttaacgaaa ctgcctttcc
    25141 cgcgaacgcg ataaataagt cctaattgct ctaattcagt caatgcacgt ttggaagtga
    25201 ttctgcttac tttataggta cgtgaaagtt ctttttcagt tggtagttgg ctgtctacag
    25261 gtaattcacc agttaaaata gccgtttgca aatcattaaa tattgtctgg tacaatggtt
    25321 gagtcatata aacacctctt aatgatatat catattcaaa taataccata atggatgaaa
    25381 aagtaaataa aaagatattt tttgaaagcg tagacataat tggctgtatg aagtataatc
    25441 tttaaagata tatcaaatag aaagaaggtt ttattagtgg tttataaaga tataccaaaa
    25501 tcggtgcaga aatttatgga agatatcaca caaaaatgtg gggatatcca tgcggactgg
    25561 gcaaagaatt ttacagcagc atttgccaat actctgttga caacagtcaa aaagcaaaat
    25621 gacggaacca cctttttatt gacgggagac attccagcga tgtggttaag agactctact
    25681 gcacaagttc gtccttattt agtgattgca aaagaagatg aggaattagc tgacatgatc
    25741 agcgggttag tcaaaaagca atttttttat atcaatatcg atccatatgc gaatgccttc
    25801 aacgaagaag ctaacggtgc tggccaccaa acagatcata caaaaatgaa cgattggatc
    25861 tgggaaagaa aatatgaaat tgattcttta tgctacccaa tacagttggc ctatcttctt
    25921 tacaaaaata caggaaaaac tgatcaattc aatcaatctt ttattgaagg tatcaaaaaa
    25981 atcttggacg tgttcaaaac agagcaagat catgaacact ctccttatac atttgtacga
    26041 gacacgacaa gaaaagaaga tacgctgatc aatgacgggc gaggtgcaaa aacagctttt
    26101 acaggaatga catggtctgg tttccgtcct agcgatgatg cttgccgtta tggatatctg
    26161 gtaccatcca atatgtttgc ggtggtcgtg ttaggttatg tggaagaaat ttttcaaacg
    26221 atactcgatg ataaagaaat tgtacagcaa acacagaaat tgaagaaaga gatccaagag
    26281 gggattcaga atcacggacg aacggttaat caaaaagggc aaacgattta tgcatatgaa
    26341 gtagatggaa tgggaaacgc cacagtcatg gatgacagta atgtgcccaa tctgatcgcc
    26401 gcaccttact tgggatattg ttctgaacaa gaggaagatt atctgcaaac aagaagaaca
    26461 ttgttgagta aagaaaatcc atatttctat gaaggaaagt acgcaaaagg aattggatcc
    26521 tcccatacgc cagaaaaata cgtttggccg attgcattag ctatggaagg aatgacaacg
    26581 tcagacaaat ctgaaaaaga gcggatattg aatttacttg tagcaactga cgcaggaaca
    26641 caccttatgc acgaaggatt cgatgtgaac aatcctgaaa attatacgag agaatggttt
    26701 tcttgggcaa atatgatgtt ttgcgaatta gtcatggatt actttgatat tagagtagaa
    26761 aagtaggagg tatcgtaaat gaaaaagaaa gtatatatca tttctcatag ccactgggat
    26821 cgtgaatggt acatggctta cgaacagcat cacatgcgac tgattgaatt gatagacgat
    26881 ctactagagc tatttgaagt tgatccgtcc ttcaacagtt ttcatttgga tgggcaaacg
    26941 attattttag atgactatct tcaagtaagg ccagaaaaaa gacaggcaat ccaacaagca
    27001 atcaatgaag gaaaattgag aattggacca ttttatatct tgcaagatga ttttcttatc
    27061 agctcggaat cgaacgtgag aaatatgctg attggcatgg aagaaagccg taaatgggga
    27121 acccctgtca tgttaggcta ttttcccgat acatttggaa atatgggaca gacgcctcaa
    27181 ttaatgaaac aagctggtat ttcagcagca gcgtttggcc gtggtgtaaa accaattggc
    27241 tttgataatc aagttttgga agcagaaaac tattcttccc aatactcaga gatgtggtgg
    27301 aaaggccctg atcaaacagc aatctttggt ctgctatttg ccaattggta tagcaacggg
    27361 aatgagattc cagtagaaaa agaagcagct cttgcctttt ggaagcaaaa attagcggat
    27421 gcagaacaat atgcctctac gaaccactta ttgatgatga atggtgttga tcatcaaccg
    27481 gtacaaaaag atatatcaaa agcaatccat ctcgcaaatg aactgtttcc agattacgag
    27541 tttattcatt ctaattttac tgactatctg gaagcagttc aaaaagatgt gccagaagac
    27601 ttaggctcag tagaaggaga attgacaagt caagaaacgg atggctggta tactttagcc
    27661 aatacagcat ctgctcgggt ttacttaaaa caatggaata ccaaggtcca gcgacagcta
    27721 gaaaatatta cagaaccact tgcgacgatg gcttatgaag ttagtggaaa ctatccccat
    27781 gatcaattag attatgcttg gaagacatta atgcaaaatc atcctcacga cagtatttgc
    27841 ggttgttcag tcgattctgt ccatcgggaa atgatacctc gttttgaaaa agcagatgaa
    27901 gttggaaagt atcttgcaca agattcctta gaacagctga ccgcagcaat cgatacgacc
    27961 ggattcccaa aagacagctt tccatttgtc attgtaaata ctgcaggaat ggataaaact
    28021 ggtgaagcag aaataaccat cgaattagaa agaaaaagat tcgctgaagg aatacctgaa
    28081 caactatatc aagagttaga aaatttgcct aaacgtaagt atcatgtaga aacaaaaagt
    28141 ggggccacta taccagctat tttatctgaa gaaactgtcc aatttggtta tgatttgccg
    28201 aaagaccgtt tccgtgtgcc atatatggca agaatgatca aagtgacttt accattagaa
    28261 aacatgccag cattttcttg ggaaacattc gcgcttgtag aaggggaagc gaaagcagaa
    28321 gaaaaggaaa caatgattca tcagagtgga cgaatcatcg aaaacggccc tctacatttg
    28381 acgattgaaa aaaatggtac cattacaatg gaagatcgca aaaacaaacg aaagttgaat
    28441 aatctgcata tttttgaaga tatcggcgat ataggaaatg agtatatctt taaacaacct
    28501 ttctgtgatc agccaatcct atccagtaac aaagaaaact ctgaagtaaa agtattagta
    28561 gatacaccgg aaatagcaaa aataagtatt ctacaggaaa tggaaatccc tgtttctgca
    28621 gacgagcgtt tagaaaaaga acagcagatg gttgtggaat ttcgttatcg aaaagcagaa
    28681 cgatccaaag aaaaacgaat ccttcaaata aaaacaatca tgacaatcag aaaagatagt
    28741 aaaaaaatcg actttgaaac gactctcgac aatcagatga aagatcatcg cttacgtgtc
    28801 ttgtttccta caaagttaca tgtggaacat cacgaagcag atagtatttt tgaagtggtg
    28861 aagcgcccaa accatgtgtc taaaagttgg gaaaacccca cgaatccgca acatcagcaa
    28921 gcatttgtca atattcatga tgaagagtac ggtgtaacag tcggaaactt tggtttgaat
    28981 gaatatgaag taacagaaga cggacaaatc gcagtgacgc tacttcgcag tgtgggagaa
    29041 ctaggcgatt ggggatattt tccaacacca gaagcacagt gtctaggtga gcatcggttc
    29101 aattacagca ttgaactaca tggaccagaa gaaaaattct ctacttattt gcatgcatac
    29161 gcagcacaga ttcctttttc cactcaacaa atcaaacatc atgaaggaac attgatttca
    29221 aaacaacaat atttaacgat aaaaagtgaa acgtttgcaa ttacagcttt gaaacgcagc
    29281 aagtttagtg ataaagtagt agtaagaggt ttcaatatgt ctagtcattt agaaaaattg
    29341 gaaataacta aagacaatgg aaaaactgtc atattgaatc tgcttgaaga accaacgaag
    29401 caagcggttg tcccaataat ccaaccttat gagattcgga caatcggatt cgaggaggaa
    29461 aacgaatgta tggcggaata gaagcaggcg gaacaaaatt tgtgtgtgcg gtttcagatg
    29521 gtggagaaat tttagaaaaa ttaagtatcc ctaccacaac accagaagaa acattggatc
    29581 aggtatttga attttttgat cgttatgaat tgaagggaat cgggattggc tcattcggcc
    29641 cgattggtat cgacaaaaca aaagataatt acggatacgt tttggcaact cccaaaaaag
    29701 ggtgggcaca attcgatttt cttggcacaa tcaaggaacg ctacaatatt cctgctgctt
    29761 ggacaacgga tgtcaatgct gctgcatatg gggaattgaa gcaaggagca gcacaaggaa
    29821 aaaatagttg tatttacctt accgtgggaa caggaatcgg tgcaggtgtc gtgatcaatg
    29881 aagaaatttt ttcagggatc gcacatcctg aaatgggaca tatttgggtg aaacgccatc
    29941 cagatgacca ttatgaaggt acttgtcctt atcataaaga ttgtttagaa ggattagcag
    30001 caggaccgtc tattgaggca cgcacaggga tcaaagggca agatctgccg caagatcatc
    30061 ctgtttgggc tattcaggct tattatattg ctcaagcact gatcaactat acgttgacgt
    30121 tagcacctga aaagattatt ttaggcggtg gggtaatgaa tcaggatcat ctccttcaaa
    30181 aaatccgtca gcaatttgtt gaattgatgg gtggttatat ggaaacacct caagtaagtg
    30241 aatatatcgt tcgttgggga ctgccgaatg aaagtgggat tatcggtagt ttgctgttag
    30301 cagaacacga acatcaaaag taagcagaca aattacacaa aaagaacaga gtcttatcat
    30361 tatctgctga taagactctg ttctttttta tttctattcc cataatccag gtaaagcttt
    30421 accgactatt ttttcttgag taaatggatg gatcatgtgc agttctaatg catggagcat
    30481 cagtcgttca gctgttctat tttggtagag agggtctcca acgatcggat gtcccttatg
    30541 agctaaatga actctgatct ggtgtgtacg tccggtttcc aaaacacaat agacttgtgt
    30601 ttgttcagaa agtactttgt tgacagtgac atgtgtcaag gcgctctgac catttttatc
    30661 atctacgacc ctttttctcc gatcatggcg atctcgtcca attttattgg tgatggtcat
    30721 atctttggca atttctcccc atacgatggc ttgatatctc cgatagatca ttttttgttc
    30781 caacattctt ccaagtatcg gcaagacaaa tggatttttt gcaaataaaa cggcaccgct
    30841 tgtttctttg tccaagcggt ggacaacgta aggtcgttgc tgtttagtgt cgagataagc
    30901 agctaaatga ttgagtaagg tactgttttc tgtcggttca tttggatgag tcttcattcc
    30961 tgcaggctta tccactataa tcaaatgctc atcttcatat aaaacacgga tgtgcttcgc
    31021 atctcctaat tgaacttctt gataattata atctgtttct tcgaataaca atgtgatctg
    31081 gtcattgccc tttacttcat gatggaacaa agcaggttgt tgattgatcc aaacgttttt
    31141 acgtatacgc aaaaagtgtc gaacctttct aggaactaac cattcctttt caagcaagtc
    31201 gcgaattgtt gtttcaggat ggttcatagg taaagtgatc gtgattttca caagtgtact
    31261 cctttcagtg caattgcaga ctaattgtac catttttaaa acatatttaa gaaaaaactt
    31321 acatttttct gttcattctt atgttactct acttgcacta acgtaaaaaa acatattgaa
    31381 ctacaactta agcaggaaga aaacactctt tcactatggt aaaataagac gattgaatca
    31441 aatagacagg agaagaagca aatggatttt caaagcattt tagaaaagat aaaagctgct
    31501 ttgatcagtt tttggcgttg gatcagacca tatctcggac aattccatcg atggcgaaag
    31561 cgcatctgga aaaaatatca tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc
    31621 ttagtgacaa gtatctacct ctttatttta gcaaaacaag caaatgtcga aacgttaaaa
    31681 tcaggattaa gccaatcgac agtcatttat gataagaaaa acgaagaagc tggcacactg
    31741 tacgcccaaa aaggaacata tgtagaatta aacgcgattt ctccctatat ccaagatgca
    31801 gtaatctcga cagaagatag gaatttttac gagcatcacg ggtttgatat caaagggatt
    31861 gcccgtgcag cagtccgaat ggtcttgtct ggcggaacat cagggggcgg aggaagtacg
    31921 atcactcagc agcttgccaa aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa
    31981 gcgaaggaat tatttttggc aatcgagatc gaaaagaagt acagcaaaga agaaatcttg
    32041 accatgtatc ttaacaatgc ctattttggc aacggggtat ggggtgtcca agacgcttcc
    32101 agaaagtatt ttggagtaga tgcaacgaat gttacgctgt ctgaggctgc tactttggca
    32161 ggaatgctga aaggtcctgg tatttataat ccgatcgatc acccaaaaaa tgcgaatgat
    32221 cggaaaaaca cagtactaag tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa
    32281 caagcagaaa gccagacaga tatcagcgcg tatcttaatg acacatacga taattcagat
    32341 tctggttatc gttatcctta ttattttgat gcagtgatcg atgaagcagt taacgagtat
    32401 ggtcttgatg aagaagatgt catgaataaa ggctataaaa tctatacagc tttagatcag
    32461 gattatcaaa agcaattaga agctacttat caaaacagtg cattattccc agcaaatgct
    32521 tcagatggtg ccatggtcca atctgcttca gtagctttag atccgaatac aggtggtgta
    32581 caagcactag taggacgaag aggagatcat gtctttagag gattcagctt tgctacacag
    32641 atgcgtcgtt caccaggatc aacgatcaaa ccattaagcg tatatacacc agcactggaa
    32701 gctggataca agccttccag tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat
    32761 aattttgacg ggacatacca aggagaagta ccgatgtatc aagctgttgc acaaagtttg
    32821 aatttgcctg ctgtatggct tttgcatgag atcggtttac aaaaaggata tgataaagca
    32881 gaagaattcg gcctgccttt agcaaaatct gataagtatt atggcttagc ccttggtgga
    32941 ctggaaaaag gagtatctcc attgatcatg gcgggtgctt acagtgcatt tgccaatgat
    33001 ggtcagatgt atacgccgca tcttatcacg aaaatcgttg attcgactgg agcggtgatc
    33061 gtcgataaca caaacaaaaa accaaaacag gtcatatcaa aggaaacagc cgatgaaatg
    33121 accagcatgc ttcttggtac attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt
    33181 tatacgatag cagggaaaac aggaaccaca gaaactaatt tcgatgccac aaaagcaaat
    33241 gaccaatgga tgatcggtta cacgccagac gtagtgattt cgacatggat gggttacgaa
    33301 gaatccagca agctgcacta tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc
    33361 aaatcagcgg cagaaggcat ccttccgtat acttcaaaaa caggatttag tgtagcagat
    33421 gcctatgcca caggaggaaa agtggtagct gcagatcaag ttggaaacac aggaaattct
    33481 aatggagagt ctggaaattg gcaagataac ttgaatcaat atggtgaaca agcaaaagaa
    33541 gggctgaaaa acttcggcga tatggtgaaa gaaggcgttc agggaattgg tgaagcggct
    33601 aaagacctct ggcggaaata tcaaggagag tagccccttt gaaaacgaat aaccccatgt
    33661 tacaatagag agtaactact aaaagaaaag agggcataac atgtcaaaca tctacgatag
    33721 cgcgaatcaa atcgaacgtg agatccgtga attaccagaa ttcaaagcgt tgaaagaagc
    33781 gtacgagaaa gtacaagcaa acgaagaagc acacaaactg ttcaaagact tccaagcgat
    33841 gcaaatcgaa ttacaagaaa aacaaatgag tggacaagag ttcagcgacg aagatgcaat
    33901 gaaagcacaa gaaatggctg tgaaaatcca atcagaagaa gtaatcaatg acttgatgca
    33961 aaaagagcaa ggcttcagta caatcatcaa tgacttgaac cgtatcatca tgacaccagt
    34021 tcgcgacctt tacagcgaat aaaactattt tatgaaacaa cagagggtgt gaaagagacg
    34081 ttcggccttg agcgatgaca aaattgcttc ttattgccga taggctgtct cttgaacacc
    34141 atttaccaag aggtcgtgag cctaccgttt aactctgagt tacaaggaac aatttcaaaa
    34201 aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg caggagaaca
    34261 ccgtttattc agaaatagga gctacgacaa aagtcttgtc atagctccta ttttttgtca
    34321 ttcatttttc aagcggtaga aatagccttt ttgagctaac agtttttcgt aagtacctat
    34381 ttcaacgatt tttccttcat cgatcacaac gattttatct gcctctttga tcgttgaaag
    34441 ccgatgagcc acaataaaag tagtgggtgt ttctgtcagg taattcaggg cttgctggat
    34501 ttttttctct gactggttat ctaaagctga ggttgcttca tctagcaaaa tgatttttgg
    34561 ctgacgtatc aacgctcgtg caatggagat acgttgcttt tggccaccag acagcttgtt
    34621 gccatgttcg cctactaagg tatccagacc atcaggaagc tggtcaatca gtgaagaaag
    34681 ttgcgcagct tcgatgactt catctaaacg ttcttttgat acgtttttca agccataagt
    34741 gatgttctct ttgatcgaag cggagaacaa caaagtggtt tgaggaacaa cggctaaata
    34801 atttcgcaca cttcgcatat ccagttcatc caaaggctga tcatctagaa aaatttttcc
    34861 tgaagtcggc tggataaatc caatcaataa gttaaggagt gtagattttc cagagccaga
    34921 tggcccaaca aaagcaattg tttctccagc tttcacctct agagaaaaat gatcgataat
    34981 cggtttgagt gagtccggat aaagaaaaga aacatcctca aaacgaaaat ttccatccaa
    35041 atgttggatt tttttcttac cgtgataagt ttctacatcg ccagcaatca acagttcgcc
    35101 aatagaagtg atagattcca tgccttttga aatcgtagga atcagcgtga tcattccaga
    35161 aacggaattg acgatcgtcg tgaaatacgt ctgatacatc acgacatcgc cgggttggat
    35221 gcgcccttgt aaagctaata tcccagtgaa aatcaaacaa atcagctgaa atatctgaaa
    35281 actagcccag ctgactgatc cgaaaacaga ttgcaggata tctaagtgga atcctttttc
    35341 atagatttga gtaaaatgct tatttaagcg agttgtttcg tgtttttcta acccatgagc
    35401 tcgagcaatc ggtgctaatt cgatcacttc gatcactttt gcagaagtct cttccatttc
    35461 ttgcctgaag tctgagttgg actgtctgat ccttttacgg aaaaagagag taacgaatgc
    35521 ggcgagagga acagtcaata agaaaaacat gaaaacaaca ggacttttat aaagcgtgat
    35581 acctaaagct acgaataaat tgacagcgat atttaatccg ctgacaaata cttgagagga
    35641 caacgtttgg atagcttcta catcacggat gatctttgac tggattcgtc cagattgcaa
    35701 ttctttttga taaggaatag atagttcttg gattttcttg ataagggtcg ctcttagtcc
    35761 agcttctact tgacggatcg aagtgctatg gtattttaca tgaaaataat tcgtcaatat
    35821 attttgacca atcagacaag ccatcaaaaa agcgttgaaa taaatggcct gcaactgaga
    35881 agaatctttc gatgaagctg cattgattac attggcaata gcgatcggca ttaaccaaga
    35941 tggaagatgt ttgataataa aaaagagtcc tgacatggca aaacgccaat aatttccctg
    36001 ataaagatgc agcaatgtct gaaaagcatt tgcatgatat ttttgataac ttttctgtat
    36061 tcgttcctcc ataatcttct ccttaacaat cttggtaaat aaactgcaag tgttctattg
    36121 cttcaggaac ataaatctca ggagtagctt caagagtggc gtacattaga ggacgctcat
    36181 atttgatata atgcagtacc gggcggaaat ctaaacagcc cttgccaaca ggtaccatgc
    36241 ggatctttcc attttccatg atatagtctt tgagatgaag cgctgtaata taaccatgta
    36301 aattgtccaa ggcacctcgg atcacttttt cctgatcagc atgattttct ttatgcatca
    36361 aattcgcaca atctaaaatg atttttaaat ttggtgaagc tacttcatcg attaaacgtt
    36421 tagctaactg atagttatag attggatgat tgatccctgc ttcaacagca acagttacgc
    36481 caaaattttc tgcatgagcg actaattcaa tgatagaatt tctggcgatc tgataagctt
    36541 cttcagtaaa attttctttt gtgtaaccga cagagacact accggtttcc gtagcaatca
    36601 tcttggcttg ataggcagag cacagagcaa tttgctgttt gaaagttgcc aatgcttgtg
    36661 cacgaacttc ttgatttttg ctggataggt tgatatagca tcctaaaatg gaaagttcga
    36721 tattgttttt ttggaagtat tcaccaaaat aggcagcact cccaagtgtc atgtcagtta
    36781 atttaagttg ttcaggaaat gctttttgtg gtgcaaattg gatatgggac aagtgaagct
    36841 gtgatacttt ttgaacgatt tcttcacgat ttcgaacatc taaatcgtgg gcacggatgc
    36901 ctaaatttaa tggaaacatc tcaaactcct ttttgtaaat aattagcaag aggcagacgg
    36961 gattctttta tcaattcaac aattattcgg gcaactttat cagcgccttc tggactaaag
    37021 tgggtattgt cttttagtcc ttctgggtaa tttttacaag tatcaggcat aagatgcagg
    37081 tagaattttt ttgtctcttc ttttgagaag gtctggaaca attcttgtga tcttgagaac
    37141 acatctaaaa gaggaatatg gtgtttctca gcaaatgcac gcatcgcttt tggatagtct
    37201 ccaagtatat ctggattcaa tgttccgttt tctaagtaat ctcgtcgcgt cacagatgtc
    37261 agtaagacca tttggacgtt cgcttttttt gcgcattcaa aaaatatctg cagatttttt
    37321 aaatagctac cgaagggaag cgtacctctt tcttcttgga ttttctggtc gttatgtcca
    37381 aattgtacaa ataagaaatc attcggctga aactgtgaag ctagacgggc taaccttcct
    37441 tcctggataa atgatttggt gctgcgtcca ttctttgctt ggttattgat ctggatctcg
    37501 ggtgaaaaat aagcagagat tttttctccc caacctgtct cgggacgctt gttttcatct
    37561 ttttctgccg cagtagaatc accggcaata tggatagtag gcatgttatc tcttctttca
    37621 taaaggttta taggcaatga agcagaacag caagagaaga agacggagca aatttggtcc
    37681 gtcttcttct ctaaaacgtg gataaacgga ttccgttatt tcactatcct gtttttgatt
    37741 cgttactgtt actattaatc aattaggcaa aaaagccaaa gtaattttta gcatttgtat
    37801 aactaatcgc ttgaactaga cttcctaaaa gttgttcatc ttcaggtgct tctcctcgca
    37861 tgaccatttc gcctaaaaat tcacaaagaa cacgacggaa atattcatgt ctagtgtagg
    37921 acaggaaact tcttgaatct gtcagcatgc cgacaaaatt ccccaaagca ctaccatcag
    37981 caaattgtgt caattgatcg cgcataccag ctcgagtatc gttgtaccac catccagaac
    38041 ctaactgcag ttttccattc tgttcttttt ggaaacagcc catcaaagct aaaatcgcag
    38101 gatagtcatt cggattcaat gaataaagga tcgtctttgg caactgatcc gtttcttctg
    38161 cacggttcaa taatttttgc agaggaatag tcaaagaaag atcattcatt ccatcatagc
    38221 cggtatcggg ccctaatttt tccaacattt gtgtattaca gttacgataa gcatggatgt
    38281 gcagctgcat cgtccaatta tgagaatgat agaagtgggt caaatgtatc agtgtttctg
    38341 taagataagc atcgatttca tctgaagata atagtttgtt agctcgtgct ttttgaaaaa
    38401 tctcttctaa tactgtttca tccgcttctt tatagcgtaa gatgtctaaa gcgtggtcgg
    38461 aaacacgaca tccatgttga tggaagtatt ctatccgctg ccctaagact tctatcagtt
    38521 cttgataggt ggtgatcgtt tggtcagctg cttcttccag tagtgtgacc catgcattaa
    38581 atccttcttt ttcaatattg agtgctttat ctggacggaa tgaaggtaga accttcaaat
    38641 ctggttcttc atgagcaagc aactgatgat agtgcaagtc gtctgcggga tcatctgttg
    38701 tacaaatcac ttctacattc gatttttttg cgatttcccg acggcgaaaa tcaggagctg
    38761 ccaaaatttc atttgcttgt gtccaaattt tctctgcatt ttcttcattt atcaacaagt
    38821 caataccgaa gaaacgcttc aattcaagat gtgaccaact gtaaagcgga tttccgataa
    38881 tttttggtac ggtttgacac caagctaaaa atttttcaaa atcagaagca tttcctgtga
    38941 ttttttcttc aggcacgccg caagctcgca tcaagcgcca tttgtaatga tcgcctccta
    39001 accaagcttc tgtcagattg ctgaaattgt tgttttcgta aatttctttt ggatctaagt
    39061 gacagtgata atcaataatg ggcatgtttt ttgcgtagga atggtaaagc tttttcgccc
    39121 aatcgtcttt taat
//
LOCUS       DANKHK010000015        38086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000015 DANKHK010000000
VERSION     DANKHK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            244..2010
                     /locus_tag="PW109_000701"
     CDS             244..2010
                     /locus_tag="PW109_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2510573.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2112..3371
                     /locus_tag="PW109_000702"
     CDS             2112..3371
                     /locus_tag="PW109_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2510574.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3518..4819
                     /locus_tag="PW109_000703"
     CDS             3518..4819
                     /locus_tag="PW109_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510575.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4822..5673
                     /locus_tag="PW109_000704"
     CDS             4822..5673
                     /locus_tag="PW109_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510576.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5686..6501
                     /locus_tag="PW109_000705"
     CDS             5686..6501
                     /locus_tag="PW109_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2510577.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6652..7818
                     /locus_tag="PW109_000706"
     CDS             6652..7818
                     /locus_tag="PW109_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2510578.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTAAKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8048..8695
                     /locus_tag="PW109_000707"
     CDS             8048..8695
                     /locus_tag="PW109_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2510579.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8779..9723
                     /locus_tag="PW109_000708"
     CDS             8779..9723
                     /locus_tag="PW109_000708"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2510580.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9858..11309
                     /locus_tag="PW109_000709"
     CDS             9858..11309
                     /locus_tag="PW109_000709"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2510581.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11330..12165
                     /locus_tag="PW109_000710"
                     /pseudo
     CDS             11330..12165
                     /locus_tag="PW109_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            12165..12824
                     /locus_tag="PW109_000711"
     CDS             12165..12824
                     /locus_tag="PW109_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2510582.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12950..13639
                     /locus_tag="PW109_000712"
     CDS             12950..13639
                     /locus_tag="PW109_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510583.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQNPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTESLE"
     gene            13645..14601
                     /locus_tag="PW109_000713"
     CDS             13645..14601
                     /locus_tag="PW109_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2510584.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWKVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14645..15157
                     /locus_tag="PW109_000714"
     CDS             14645..15157
                     /locus_tag="PW109_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510585.1"
                     /translation="MYIRQTRKEEVKEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15413..15961)
                     /locus_tag="PW109_000715"
     CDS             complement(15413..15961)
                     /locus_tag="PW109_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2510586.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKEILQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGTFQEAADKIKKGLLI"
     gene            complement(16050..17231)
                     /locus_tag="PW109_000716"
     CDS             complement(16050..17231)
                     /locus_tag="PW109_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2510587.1"
                     /translation="MGYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17466..18974)
                     /locus_tag="PW109_000717"
     CDS             complement(17466..18974)
                     /locus_tag="PW109_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2510588.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19133..19324
                     /locus_tag="PW109_000718"
     CDS             19133..19324
                     /locus_tag="PW109_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510589.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19527..20444
                     /locus_tag="PW109_000719"
     CDS             19527..20444
                     /locus_tag="PW109_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2510590.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVESISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALQQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20437..21510
                     /locus_tag="PW109_000720"
     CDS             20437..21510
                     /locus_tag="PW109_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510591.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21658..22176
                     /locus_tag="PW109_000721"
     CDS             21658..22176
                     /locus_tag="PW109_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510592.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22157..22549
                     /locus_tag="PW109_000722"
     CDS             22157..22549
                     /locus_tag="PW109_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2510593.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQLASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVEEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22625..23560
                     /locus_tag="PW109_000723"
     CDS             22625..23560
                     /locus_tag="PW109_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2510594.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23693..24472
                     /locus_tag="PW109_000724"
     CDS             23693..24472
                     /locus_tag="PW109_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2510595.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24465..24782
                     /locus_tag="PW109_000725"
     CDS             24465..24782
                     /locus_tag="PW109_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2510596.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25026..26636
                     /locus_tag="PW109_000726"
     CDS             25026..26636
                     /locus_tag="PW109_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2510597.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTIQSTM
                     LQSLNHHRFAIRLTVEAIILKVIFEVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAIFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26793..27641
                     /locus_tag="PW109_000727"
     CDS             26793..27641
                     /locus_tag="PW109_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2510598.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHVDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27872..28351
                     /locus_tag="PW109_000728"
     CDS             27872..28351
                     /locus_tag="PW109_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510599.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28384..30612
                     /locus_tag="PW109_000729"
     CDS             28384..30612
                     /locus_tag="PW109_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2510600.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKVSNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDPDELAIL
                     EQALSDSGLLLELQDILRKEKWGHLQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEFPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPSFQGKTPEVRELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30741..32384
                     /locus_tag="PW109_000730"
     CDS             30741..32384
                     /locus_tag="PW109_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2510601.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGNGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPEF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32457..33002)
                     /locus_tag="PW109_000731"
     CDS             complement(32457..33002)
                     /locus_tag="PW109_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2510602.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEARLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33025..33510)
                     /locus_tag="PW109_000732"
     CDS             complement(33025..33510)
                     /locus_tag="PW109_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2510603.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33500..34312)
                     /locus_tag="PW109_000733"
     CDS             complement(33500..34312)
                     /locus_tag="PW109_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2510604.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34451..35983)
                     /locus_tag="PW109_000734"
     CDS             complement(34451..35983)
                     /locus_tag="PW109_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2510605.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRACQLAHLGVKSGYKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPDLSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEVHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(36092..37123)
                     /locus_tag="PW109_000735"
     CDS             complement(36092..37123)
                     /locus_tag="PW109_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2510606.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFRTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFELTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVQVSSI"
     gene            37476..37850
                     /locus_tag="PW109_000736"
                     /pseudo
     CDS             37476..37850
                     /locus_tag="PW109_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
BASE COUNT        12448 a         6825 c         7580 g        11233 t
ORIGIN      
        1 tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga
       61 cactaaatag tacaggaagt agattaaaac ttttaaaagt tttaagtcta tctatttggc
      121 aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg
      181 ttgtcttttg tttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa
      241 tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg
      301 aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg
      361 ctttgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg
      421 aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca
      481 aggcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt
      541 gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga
      601 ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta
      661 tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac
      721 actttacctt ggggaagtaa agatccagac cgagaagact tcttcggggg agatttgcaa
      781 ggtgtgaggg atcatttgga ttacttgacg gaccttggcg tcaatggcat ctatttttgt
      841 cccatattta aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat
      901 cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg
      961 atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa
     1021 gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc
     1081 ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat
     1141 acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa
     1201 tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt
     1261 gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa
     1321 atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa
     1381 ggggatgaat tccatgcagt gatgaattat gcattcacag attcgattaa agattatttt
     1441 gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt
     1501 tacagagatc aggtgaacga aggaacattc aacttattag attctcatga tactgccagg
     1561 atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt
     1621 cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat
     1681 gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc
     1741 ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt
     1801 tctactcagt ggctaatagt atatgatcgt gaagataagc tctatttcac acgggaacta
     1861 gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag
     1921 gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata
     1981 cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt
     2041 ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacacagg
     2101 aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag
     2161 gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg
     2221 atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa
     2281 taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt
     2341 tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga
     2401 tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac
     2461 tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa
     2521 tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg
     2581 atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt
     2641 ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt
     2701 cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt
     2761 cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt
     2821 tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc
     2881 agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat
     2941 acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc
     3001 cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag
     3061 acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg
     3121 aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg
     3181 atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc
     3241 cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt
     3301 caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac
     3361 aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag
     3421 ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag
     3481 cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa
     3541 tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa
     3601 tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat
     3661 gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga
     3721 agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt
     3781 cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga
     3841 gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt
     3901 tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt
     3961 cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag
     4021 tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg
     4081 taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt
     4141 gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa
     4201 acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat
     4261 gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt
     4321 cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg
     4381 gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat
     4441 ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg
     4501 agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc
     4561 gccaacattt attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt
     4621 taacaatggt ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc
     4681 ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac
     4741 gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc
     4801 gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga
     4861 ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat catctatccg
     4921 cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat
     4981 ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg
     5041 acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta
     5101 gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg
     5161 atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc
     5221 atggctttct tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc
     5281 ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat
     5341 ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta
     5401 ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt
     5461 ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc
     5521 ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg
     5581 gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt
     5641 accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg
     5701 ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta
     5761 tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg
     5821 actctttttt atgccaatca gatccaagcg attccattag aacaatttct atccgttcat
     5881 tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg
     5941 ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta
     6001 tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg
     6061 atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc
     6121 caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat
     6181 gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt
     6241 gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc
     6301 caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga
     6361 ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta
     6421 agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc
     6481 aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa
     6541 atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct
     6601 tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag
     6661 aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat
     6721 tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat
     6781 atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc actaggagaa
     6841 gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct
     6901 tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa
     6961 gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac
     7021 gacttttata aactttggct agatccaact ttgacttatt cttgtgctta tttcaacaca
     7081 cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc
     7141 tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc
     7201 actgctgcca aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac
     7261 gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta
     7321 atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc
     7381 gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa
     7441 ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct
     7501 tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat
     7561 aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa
     7621 ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa
     7681 atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt
     7741 gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc
     7801 ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct
     7861 aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag
     7921 cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat
     7981 gacagaaaat tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat
     8041 cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc
     8101 ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg
     8161 ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa
     8221 aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga
     8281 attgttccaa gataaatacc cagatactgc aattactagt ttggaattag atagtgattg
     8341 gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat
     8401 tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca
     8461 aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga
     8521 agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga
     8581 taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc
     8641 agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt
     8701 tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg
     8761 tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat
     8821 cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct
     8881 cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat
     8941 cccgaaccac gctattttga tgttccttta gggagtatca attctatggg acttccaaac
     9001 ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa
     9061 aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt
     9121 gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat
     9181 gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa
     9241 gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct
     9301 cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc
     9361 aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca
     9421 aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt
     9481 cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg
     9541 acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtaca
     9601 gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt
     9661 atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca
     9721 tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg
     9781 ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag
     9841 gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag
     9901 aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg
     9961 aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc
    10021 cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag
    10081 aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc
    10141 atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca
    10201 atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa
    10261 acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt
    10321 catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg
    10381 cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat
    10441 atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag
    10501 acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat
    10561 cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg
    10621 attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag
    10681 atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta
    10741 aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga
    10801 ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt
    10861 tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg
    10921 atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa
    10981 cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac
    11041 agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt
    11101 ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag
    11161 caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg
    11221 ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc
    11281 agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa
    11341 aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc
    11401 cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc
    11461 gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta
    11521 tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga
    11581 atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa
    11641 cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata
    11701 ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga
    11761 tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa
    11821 acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt
    11881 agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc
    11941 tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag
    12001 tggtttttta ttgaaagaag gaaatgatgc ctgaaaaaga aaatcgatgg cgtattaaaa
    12061 gagatgaaag aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt
    12121 tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcctctaa
    12181 ttgatttttc cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat
    12241 tagggattgg cttcgtgtcg tttcttttag gaaatttggt agggttgctt ttgacagttt
    12301 taggtctttt ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta
    12361 gaggcattcc cgcactagtc ctgctgtttc tgttatattt cgggttacct tatcaattaa
    12421 gtgccttaac ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa
    12481 tctatcgagg atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg
    12541 gtttttcttt ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcgg
    12601 taccggcact cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa
    12661 tcacagtgcc agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt
    12721 tttctctcta tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag
    12781 tgcaacagta tttagaaaaa cgttctatcc atcagtcaat ctgaaaaata gaaatggtgc
    12841 tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa
    12901 accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc
    12961 gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt
    13021 tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac
    13081 aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt
    13141 tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa
    13201 actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc
    13261 gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc
    13321 aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt
    13381 cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caacagatca
    13441 attgtacaaa gaattgaaaa gacgttcaga aaaccaaaat ccattttcta acgaacgata
    13501 ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt
    13561 tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc
    13621 tacagagtca ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac
    13681 ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg
    13741 atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag
    13801 caccatccaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag
    13861 catgtgcaca gttggaaagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg
    13921 ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag
    13981 cactacccaa aagatacaga ggtgtggaaa cagctggcag gagttgattc aaaatttttg
    14041 ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac
    14101 tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt
    14161 gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa
    14221 ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat
    14281 attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct
    14341 aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt
    14401 caccatcatc aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa
    14461 caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttagaactt
    14521 attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa
    14581 ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa
    14641 ctggatgtat attcggcaaa caaggaaaga agaagtaaaa gaagtggtag aaatcattaa
    14701 tgccgcagca gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg gacagaaacc
    14761 aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga
    14821 agatcaaatt gctggaacag ctgcattagt ctcaggcgtg gatcccgttt acacaaaaat
    14881 agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc
    14941 agaaagtacc cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc
    15001 agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga
    15061 aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca
    15121 cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga
    15181 taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa
    15241 gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa
    15301 ttttagcgta ttttacgaaa cagctgcttt ctgaaactcc cttcatcgag aggtcgtgac
    15361 atagcttttg tcacgacctc tcggcttatc ctacgagtat tattcgatac atttatatca
    15421 ataatccttt ttttatttta tctgcagctt cttggaacgt tccatcgttt gacacgacta
    15481 aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca
    15541 gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatattt
    15601 ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc
    15661 aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt
    15721 ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc
    15781 ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt
    15841 ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag
    15901 cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca
    15961 ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc
    16021 ttttaaaagc aactttgctt ttttgtatat tataccagca agccagtcac aatagcactt
    16081 aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt
    16141 cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg
    16201 gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta
    16261 ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata
    16321 ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag
    16381 atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc
    16441 atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa
    16501 ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg
    16561 atcagctcat cttctttttc ttctaataca tagggattta caattgaagc cagaatataa
    16621 ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata
    16681 taagatccga cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa
    16741 cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct
    16801 aaaatagcag aagctactcc attataagat tccaatttgc ccatcccgtt gattttactt
    16861 aatcctagac caacagcttt cataaataat ggaagaatct tgatatagcg taaaatcccg
    16921 ataatagcag aaataaatac gattggcatc aatacggaaa gaaagaaagg tgcacttcct
    16981 ttatttgcta caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga
    17041 tcaaacattt ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg
    17101 aaaccaagaa caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt
    17161 ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat
    17221 aaataaccca aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat
    17281 aaaatactaa tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc
    17341 cattagaaaa taaagcgaac aaaataaaag gttcaaaaag tgtaacgtct tagtaaagtc
    17401 tataaaaaac gaaactattg attaagctaa tagtttcgtt ttcaacattt ataagtaaac
    17461 tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt
    17521 cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt
    17581 gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca
    17641 ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt
    17701 gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca
    17761 tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc
    17821 aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca
    17881 gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact
    17941 accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta
    18001 actgcttctt catcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg
    18061 atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca
    18121 cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca
    18181 ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta
    18241 ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct
    18301 gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg
    18361 aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa
    18421 gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt
    18481 tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga
    18541 tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata
    18601 aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag
    18661 ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa
    18721 tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct
    18781 tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca
    18841 cgtttcgcat gcttgtcgtc aactagcttc ccaccaaatt ctaaatataa tttgtcatag
    18901 tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa
    18961 ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg
    19021 attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact
    19081 tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa
    19141 aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga
    19201 aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac
    19261 gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa
    19321 ataaaaaata agataaaggt cattggattc gattctgtcc tatcagatgg aaagactagt
    19381 tgatcgaaaa acaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc
    19441 tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa
    19501 cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc
    19561 cagacgaact agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt
    19621 acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt
    19681 ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat
    19741 tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttactgatgc
    19801 gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaagcatct
    19861 cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta
    19921 ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat
    19981 attctcaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga
    20041 tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa
    20101 tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc
    20161 aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata
    20221 ttgagacgcc tgaaacaatt cagcagcaaa aagaagcatt gcagcaagaa gcaagagcgg
    20281 accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta
    20341 gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga
    20401 atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa
    20461 tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa
    20521 caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata
    20581 cacgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac
    20641 gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc
    20701 agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc
    20761 gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca
    20821 atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg
    20881 ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat
    20941 ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg
    21001 accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca
    21061 atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat
    21121 cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc
    21181 catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac gaagccactt
    21241 cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc
    21301 gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta
    21361 cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg
    21421 tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc
    21481 ttggtcccgt taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc
    21541 agataattct gttccaaagg acttgcggca gttatccaga ataattgtcg caagtctttt
    21601 ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg
    21661 tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc
    21721 gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt
    21781 tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt
    21841 tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt
    21901 ttgttttcaa tcgtttgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc
    21961 tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta
    22021 tatcgtttac ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac
    22081 agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat
    22141 cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat
    22201 actcattttg acaagttgca gtacaaccta tacacaattg gcttccaacc aaaaggagga
    22261 agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga
    22321 aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga
    22381 agtcgaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa
    22441 tcaactctat tggatgttta ctgtggacgg taaaagtgct actactggtg ccaaagagat
    22501 caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag tttcatgaaa
    22561 aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa
    22621 taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa
    22681 tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct
    22741 aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga
    22801 taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata
    22861 ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat
    22921 ggaagctttt gaaaaattga cgcatccatc ccatgaagtg ggtaatccga tcattgttat
    22981 agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga
    23041 aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca
    23101 cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc
    23161 tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg
    23221 attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag
    23281 tctgatcatc ggtgaaagcg tgacagcaag cgatttgtca aaaatcaagt tagcctttga
    23341 acgacctgaa atcagtcggc taattgatat caaaacagtt catttaagtc caacggagat
    23401 tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat
    23461 caacgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat
    23521 tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga
    23581 agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt
    23641 tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa
    23701 aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta
    23761 tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt
    23821 atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat
    23881 gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg
    23941 ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt
    24001 ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc
    24061 aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc
    24121 atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga
    24181 ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag
    24241 cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt
    24301 attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat
    24361 gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt
    24421 caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact aaattatatt
    24481 tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc
    24541 tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg
    24601 tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata
    24661 ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac
    24721 gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt
    24781 aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc
    24841 cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat
    24901 tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct
    24961 tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa
    25021 tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat
    25081 gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag
    25141 atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga
    25201 tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag
    25261 aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca
    25321 tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc
    25381 cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg
    25441 ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt
    25501 atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt
    25561 attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg
    25621 cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag
    25681 acttcttcga acttaaagat tttttgatat ccagccgtta tttcgaaaga gaaaaccgat
    25741 ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta
    25801 ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag
    25861 agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat
    25921 tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa
    25981 gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga
    26041 ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat
    26101 acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag
    26161 cttctttgtt tttctcttta ttcacgatcc aatctacgat gcttcaatct ttgaatcacc
    26221 atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc ttcgaagtaa
    26281 tcggattagg attagttgga ggatacggga tgagcttatc aaatggctta gcattcggta
    26341 ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa
    26401 agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt
    26461 ttgccatttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga
    26521 tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa
    26581 aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta
    26641 actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt
    26701 ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg
    26761 gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt
    26821 cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt
    26881 tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg
    26941 gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt
    27001 tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt
    27061 cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag
    27121 taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag
    27181 ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca catgtcgatc
    27241 gaatccgaga taatacctac gcttatctga ttgtagatga tttgaacaat ttagtacgag
    27301 gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc
    27361 tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat
    27421 ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac
    27481 tttacgttat acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga
    27541 aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt
    27601 taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac
    27661 ataccaaaaa tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa
    27721 agaacgagat ggtgttttta tttctatcaa cgaagctctt tttctttgca atccacactt
    27781 caatcctcta taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt
    27841 actaaaaata aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa
    27901 accagtaatc gttgtagaag actatgataa agttgatgga agaaatgcat taaatacgga
    27961 cgcaaaaggg ctttctctgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc
    28021 aggcaaggta tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat
    28081 cacacgtatt ttagatttag caatcttagc tgcgcaagga agtttatact tccaagatgc
    28141 ttatcgctat gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattgca
    28201 agggaaccga atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat
    28261 tttgtattac gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt
    28321 aaaacgtctt ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga
    28381 agcatggaga atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg
    28441 atctggaaga aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa
    28501 attaaaggga attggcaaga tcctgaaatg aaagtcagta acgtactttt ggaaggagat
    28561 gccgggtcgg ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat
    28621 acaaaagtga cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct
    28681 gtagtagatt ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat
    28741 acacttgaag ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct
    28801 aaagaaaaat tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat
    28861 tacagctatt atccttctga aatcgttcga gcgattgaaa aaggatattt attagagatt
    28921 caagagccga ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgcttgaa
    28981 ccaaatggga tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt
    29041 gtgatcacta caaatcgaaa ttaccaagga aatcgtccat taaatgaatc tttaagagat
    29101 cggatgcaac atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg
    29161 atggcgaaaa ccatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt
    29221 ttgttggatg aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat
    29281 ttttattggg caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa
    29341 gttttgtata aattatccac tgatccagat gaattggcaa ttcttgaaca agctttgtct
    29401 gacagtggat tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatctt
    29461 caatctgagc atacaaaagg aagaaccatt agcgccgaag aagcaaatga acgaaacatc
    29521 actgaaaatg ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa
    29581 ccaaatgagg gagaagagaa acaagaagaa acaatagaaa aaaacgagtt tcctcctcaa
    29641 atgcaaaaag aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga
    29701 gatgattctc aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag
    29761 caactgaata aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc
    29821 atcgttcacc gaccatcttt ccaaggaaaa acgccggaag tacgtgagct acacatgcaa
    29881 gtaatgccaa tagtagaatc acttagccga caaattttag agttactgga taatgaacaa
    29941 acagagacgt accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca
    30001 tatggtgatt tgaggaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg
    30061 gtagctgtac ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca
    30121 aaaaaagcag cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt
    30181 tacggagata cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa
    30241 tttgaagacg gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac
    30301 aatcgtgatg gtgcggcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca
    30361 accaaactgt tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc
    30421 aaaaaagcaa aagaagatat ccagtctgtt ttgaaagaat atgagcgtca aggaatccta
    30481 tttgtctccg cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt
    30541 cgttttgtag atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc
    30601 cgctatctat gataaaataa agctcaacga acgcacttag cagattcatt gagcttttcg
    30661 tttctagtta ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac
    30721 cgttaaagga gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct
    30781 atcaaatcta tcctgctagt ttcaaagata gcaacggcaa tggtcaagga gacatcaatg
    30841 ggatccgtga aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc
    30901 cgatctatca atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag
    30961 aagagagatt cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag
    31021 ggatcaaagt gattatggat cttgtcatca atcatagttc ggatcagcat caatggtttg
    31081 aagaatcgaa aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg
    31141 tagatggcaa agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt
    31201 ctcatgaaac tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt
    31261 gggaaagcga gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag
    31321 ggatagatgg atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa
    31381 aagccacaga gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga
    31441 ccgatttgaa acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg
    31501 tcactgcaga agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg
    31561 aatttgatca tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat
    31621 tgaaacatgc attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat
    31681 atatggaaaa ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgagt
    31741 tttgggctat gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat
    31801 ttatttatca aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag
    31861 ttgatgcagt agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag
    31921 aagaagcatt ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat
    31981 ggacttcgga acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa
    32041 ataccaaaac gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct
    32101 ataagaacat gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag
    32161 aatatcttca tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat
    32221 atcttattat ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag
    32281 atcagtcctg gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca
    32341 tactagcacc ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag
    32401 ttcattagtt ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa
    32461 taattcgctc taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct
    32521 tgtccaaggg ttttcaaacg aggtctgaca ggtacttgga catgtttgat atgcataccg
    32581 attgatgtgt caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga
    32641 tcattgaact ggtcaaaagc agcaacagaa cacgcacctc cagcatgtag tgcaggaaca
    32701 acagagacaa tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg
    32761 ttcaaatgtt cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt
    32821 aacgtacgga tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca
    32881 acgacttcgc ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gcgtgcctca
    32941 gccaatatgt cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc
    33001 attttctaaa accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact
    33061 tgcagttata ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat
    33121 agaagccact ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat
    33181 cataactaag cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa
    33241 agaaccagca acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg
    33301 atatcctgaa atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt
    33361 tggaccataa agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt
    33421 cgtagccggt acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat
    33481 agcatagatc gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag
    33541 accagacgct tccggtatga tcgttcatca gcacaccatt agaaatacaa tgggcaacaa
    33601 attttttact ttctgctact gctttggtaa agtctgcccc taacacaaga ttcgctgtta
    33661 ttgcagaaga aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag
    33721 aaaatacctc ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc
    33781 ttgtgccgcc tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat
    33841 ggatcatttc tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca
    33901 aattaggcgt aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca
    33961 attgattgat cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga
    34021 tattttgttt tgtttttatg aagtctcgaa caaccatgat tgcttcactt gagttcaata
    34081 acccaatttt gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa
    34141 gcaattccaa agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag
    34201 aagtcaaggc gcttagtccg aatagaccaa aattctcaaa agtttttaaa tcagtttgga
    34261 ttcctccacc agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt
    34321 cctccatgaa taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt
    34381 tttatctcat ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt
    34441 ccttaaatag ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt
    34501 tcattgtagc tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag
    34561 gaagttcatg gacttgctcc caccatcgat tccaatccat ttctttgtca ggagctaaag
    34621 caataatcgg ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat
    34681 ctaacgaatc taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat
    34741 ccacttgccg atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc
    34801 tatcccacca atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca
    34861 aagcccgtcc tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc
    34921 caactgaatc tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg
    34981 catcacttgt tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac
    35041 tttggccata tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct
    35101 tcattgtctg gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag
    35161 cttctggatg ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta
    35221 gtgcaattgg ccggtgaact tccaacatat gaacaacttt tgaactttgt gcgtttgcta
    35281 aaggcatcat aggaaatccc atggacatca aagaagagat accaatattg aatctggaat
    35341 gcactggaga gatgtgaaaa gcgattaatt tcttttcaga aatttttgtg aaaactgttt
    35401 tttgccattt cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac
    35461 cagttgtacc agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag
    35521 aaacagacaa atcaggttgt tccattaaat aaagaatagg aatctgtttt gattttgttc
    35581 catttgaaat ttctctcact cgcttttttg tctcatcatc aaataaaatg aaagggtctt
    35641 ctaaacgatc gataaatact tccattgttg ttgttggtag atgataagaa gtcatgacag
    35701 gcactgcacc aaggtaagac acagaaacag ctagaaggta tgtgtcaaaa gcggaactct
    35761 tataaataat aactttgtat ccggatttca ctccaagatg agccaattga caagcccgtt
    35821 tcaacacact ttcgcagcat tttttgtaag ttgtttcaag tcctaattct gggaaagctg
    35881 aaagtggctc atcaaagata atcggggtct cggaaaagtt agctgcagct tcttgaaaat
    35941 tcgtaaataa gttcaaaggg gaatacgtta tggtctccat cattcacact ccattatctt
    36001 ttgttaagta atacacaaat tcaagataat cgtaacataa tcctctatat agtaacatag
    36061 tttattgtga aaaaaagatc atacatttgt tttatatgga agaaacctga acgaatgccg
    36121 ctaattcgtg ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg
    36181 agaggtatcg attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg
    36241 cataataacg atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt
    36301 tgctcaaaat ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa
    36361 caaaagaatt ttcgtccgta ttttgtgctg ttaactcaaa atagttctcc agaaacggca
    36421 tcgcttcttt tggtattggt actttattca gtctgttttc ttttctattc agattaagaa
    36481 tctgcttggt caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc
    36541 cgcaatacag aagaatggca gtaattgcaa ggttcctcgg tcgattcttt tcttcaaagc
    36601 tagcttctct gcttgttcga aagccttcaa tcgggataaa caaatgctca aaatctttga
    36661 aaagttctgc atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca
    36721 aaagagaaat ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta
    36781 atgaatattg cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt
    36841 ggttcgctgt agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat
    36901 aatcttgttt ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc
    36961 tcaaataatc agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa
    37021 gggaagaacg attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt
    37081 gctttaactt ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat
    37141 agtgttcatt ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga
    37201 tcttaggaag caaattttta actgtctttt aaaagtacta gctaaaagat ttgaagtaag
    37261 aagatacatt tttagttatt ttattagtgc agtcattctt ttgatatttt aattgattta
    37321 tcctcttttt agctatgtgg ttacttcatg agcaatgaaa tggatttggt tcgttctaat
    37381 ggcaggaggc tttttctttt atttccgaaa aattagaaaa cattttttca gaatcgattg
    37441 gctctccatt tattcaataa tggtttgtct aacgattaaa agaagaaaaa ttagatgtaa
    37501 atacacatga tgtgacaact ggagaagtcg acatccacaa acatgtggtc aacgatactg
    37561 aaacggtaga agtaccggtc aaacgtgaag aaatcgtcat cgaacgcaaa ccagtaacag
    37621 atcaatcttc acagggaaca gacgaaaact tggaagatga cacgattact atcccaatca
    37681 aagaagagca agtagatgta agcaaacata cagttgttcg tgaagaagtt gggattcata
    37741 aagaagaaca tgaagatgtt gaaaaagtga cagaagatgt tagccgagaa gaattggata
    37801 ttgacacaag cggtgatgta cacattgaag accgcaataa aaaatcgtaa gcgtaagcta
    37861 aagaggttgt gacagaagtg gtcagcttaa agaaatttcg gcttatttcc gaagaagcta
    37921 cttctggaac accgtttatt cgcaacaaaa aagggctgtg acaagacttt ttgtagtgac
    37981 ccccaaaagt tagacttttt tggagtggag aaatccactc cttttttcat gctctaaaat
    38041 gatattttta cacaaccgtt gcggttgttt tacgaaattg gacggg
//
LOCUS       DANKHK010000016        35347 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000016 DANKHK010000000
VERSION     DANKHK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..552
                     /locus_tag="PW109_000737"
     CDS             <1..552
                     /locus_tag="PW109_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2510607.1"
                     /translation="QFQTYALTNAGASQTQATGLGIILNVVALGTTIVFTSISGGKYR
                     NKAFFVGAFVQFAAMLGMVLIGGGAGFIALATTIAFYNFGNPLAGEAIYKVWTQESFP
                     AETRASLQGFINGFSRLCCGLFAFVTPYLVMPDHIQSSMIGFAWIVLVATIAGIVMMR
                     LQKKYGTSETRGTEDLQDTNVTA"
     gene            580..2166
                     /locus_tag="PW109_000738"
     CDS             580..2166
                     /locus_tag="PW109_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2510608.1"
                     /translation="MAFTFQINNDVVWDYDQKLLKKAEENIPKLIETEIQPIRIVEKI
                     PAQQRTNEFKLQDSQKDICTLAQYSLGRGESIILDLGDHYVGQFSIDIDSVGSPMDAP
                     LYLRLKFAEVPSELYARSEDYEGWLSRSWIQEEYLHLDTLPTTLQLPRRYSCRYVELT
                     VIDTSPKWQASFSSPKFIAKSSVSMAQVPTMTIEDNTLKAIYDVSVKTLLDCMQTVFE
                     DGPKRDRRLWLGDLRLQALANYYTFNDLSLVKRCLYLFGGMRTNEGKIPANVFTEPKL
                     TPDDTFLFDYSLFFVTSLYDYVAHTKDKNVLADLYLVAKKAIDLALKQVDIEGRLVLT
                     EDFPVFVDWSNDFDKATAGQAIMIYTLKRFLKLAKLSNEANEMYYQEILEKMINFSNQ
                     TLFNHEKKQFVIEKNNEINVASQVWMVLAEVFDKQTNKEIMLETIKYHFPIQGIATPY
                     MYHHVVEALFIADCQEEAIKLMKEYWGKMIELGADTFWEAFKPEDLTFSPYGSPILNS
                     YCHAWSCTPAYLIGKYLIGK"
     gene            2284..3657
                     /locus_tag="PW109_000739"
                     /pseudo
     CDS             2284..3657
                     /locus_tag="PW109_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343224.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3932..4300
                     /locus_tag="PW109_000740"
     CDS             3932..4300
                     /locus_tag="PW109_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2510609.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            4481..5422
                     /locus_tag="PW109_000741"
     CDS             4481..5422
                     /locus_tag="PW109_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2510610.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMEAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALSK"
     gene            5491..6222
                     /locus_tag="PW109_000742"
     CDS             5491..6222
                     /locus_tag="PW109_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2510611.1"
                     /translation="MKSLLYAIGDVHGKYELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLLHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            6366..8546
                     /locus_tag="PW109_000743"
     CDS             6366..8546
                     /locus_tag="PW109_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2510612.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKKVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFLQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLTDILAGLT
                     QPGRDMRVEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            8566..9048
                     /locus_tag="PW109_000744"
     CDS             8566..9048
                     /locus_tag="PW109_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2510613.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9064..9825
                     /locus_tag="PW109_000745"
     CDS             9064..9825
                     /locus_tag="PW109_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2510614.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVLEKILLMNDPTLQRRTQATFEKVW
                     KSEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            9956..11290
                     /locus_tag="PW109_000746"
     CDS             9956..11290
                     /locus_tag="PW109_000746"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2510615.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     DVPIYYYGVTENDDIQARKIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMMTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            11596..12081
                     /locus_tag="PW109_000747"
     CDS             11596..12081
                     /locus_tag="PW109_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2510616.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12074..12757
                     /locus_tag="PW109_000748"
     CDS             12074..12757
                     /locus_tag="PW109_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2510617.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     AVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            12883..15528
                     /gene="polA"
                     /locus_tag="PW109_000749"
     CDS             12883..15528
                     /gene="polA"
                     /locus_tag="PW109_000749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2510618.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            15573..16409
                     /gene="mutM"
                     /locus_tag="PW109_000750"
     CDS             15573..16409
                     /gene="mutM"
                     /locus_tag="PW109_000750"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2510619.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            16373..17002
                     /locus_tag="PW109_000751"
     CDS             16373..17002
                     /locus_tag="PW109_000751"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2510620.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17054..17386
                     /locus_tag="PW109_000752"
     CDS             17054..17386
                     /locus_tag="PW109_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2510621.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            17698..18189
                     /gene="nrdR"
                     /locus_tag="PW109_000753"
     CDS             17698..18189
                     /gene="nrdR"
                     /locus_tag="PW109_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2510622.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            18212..19585
                     /locus_tag="PW109_000754"
     CDS             18212..19585
                     /locus_tag="PW109_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2510623.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            19585..20511
                     /gene="dnaI"
                     /locus_tag="PW109_000755"
     CDS             19585..20511
                     /gene="dnaI"
                     /locus_tag="PW109_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2510624.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            20640..21080
                     /locus_tag="PW109_000756"
     CDS             20640..21080
                     /locus_tag="PW109_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2510625.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            21224..21925
                     /locus_tag="PW109_000757"
     CDS             21224..21925
                     /locus_tag="PW109_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2510626.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            21922..23043
                     /locus_tag="PW109_000758"
     CDS             21922..23043
                     /locus_tag="PW109_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2510627.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23058..24290
                     /gene="pepT"
                     /locus_tag="PW109_000759"
     CDS             23058..24290
                     /gene="pepT"
                     /locus_tag="PW109_000759"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2510628.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            24422..25330
                     /locus_tag="PW109_000760"
     CDS             24422..25330
                     /locus_tag="PW109_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2510629.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            25365..25637
                     /locus_tag="PW109_000761"
     CDS             25365..25637
                     /locus_tag="PW109_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2510630.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            25648..26694
                     /locus_tag="PW109_000762"
     CDS             25648..26694
                     /locus_tag="PW109_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2510631.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            26945..29554
                     /gene="clpB"
                     /locus_tag="PW109_000763"
     CDS             26945..29554
                     /gene="clpB"
                     /locus_tag="PW109_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2510632.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(29705..30391)
                     /locus_tag="PW109_000764"
     CDS             complement(29705..30391)
                     /locus_tag="PW109_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510633.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(30369..30863)
                     /locus_tag="PW109_000765"
     CDS             complement(30369..30863)
                     /locus_tag="PW109_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2510634.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(30883..32103)
                     /locus_tag="PW109_000766"
     CDS             complement(30883..32103)
                     /locus_tag="PW109_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510635.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            32250..34085
                     /gene="lepA"
                     /locus_tag="PW109_000767"
     CDS             32250..34085
                     /gene="lepA"
                     /locus_tag="PW109_000767"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2510636.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(34179..35306)
                     /locus_tag="PW109_000768"
     CDS             complement(34179..35306)
                     /locus_tag="PW109_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2510637.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        11687 a         6224 c         7507 g         9929 t
ORIGIN      
        1 cagttccaaa cgtatgcttt aacgaatgct ggagctagtc aaacacaagc aactggatta
       61 ggaatcattt tgaatgttgt tgctttaggt acaacgattg tttttacttc tatttctggt
      121 gggaaatatc gaaataaagc tttttttgta ggtgctttcg ttcaatttgc agctatgctc
      181 ggaatggttt taatcggtgg gggcgcaggt tttattgctt tggcaacgac gattgctttt
      241 tataattttg gtaatccttt agctggggaa gccatttata aagtatggac acaagaatca
      301 tttcccgcag aaacaagagc atcattgcaa ggatttatca atggtttttc acgattatgt
      361 tgtggacttt tcgcatttgt tacgccttac ttagtaatgc cagatcatat tcaatcttcg
      421 atgattggct ttgcttggat cgttttagta gcaacaattg ctggtattgt catgatgcgg
      481 ttacaaaaga aatatggaac atctgagaca cgagggacag aagatcttca agacactaat
      541 gtcactgctt gaaaaattga ccaagtaagg agaataaata tggcatttac ttttcaaata
      601 aataacgatg tagtttggga ttatgatcag aaattattga aaaaagcaga agaaaatatt
      661 ccaaaactaa ttgaaacaga aattcaacca attcgaattg ttgaaaaaat tcctgcacaa
      721 caacgtacca atgagtttaa acttcaagat agtcaaaagg acatctgcac attagctcag
      781 tattcactgg gaagaggtga gtccatcatc ttggatttag gggatcatta tgtcggtcaa
      841 tttagtattg atatcgattc tgttggttca ccgatggatg cacctttgta tttacgcttg
      901 aaatttgcag aagtaccttc tgaattatat gcccgttcag aggactacga aggctggttg
      961 tcacgttcct ggattcaaga agaatatctt catcttgata cgttacctac tacactgcaa
     1021 ttacctaggc gctacagctg tcgttatgta gaactaacag ttatcgatac ctctcctaaa
     1081 tggcaagcaa gttttagtag tccaaaattt atagcaaaaa gttctgtttc aatggcacaa
     1141 gtacctacga tgacgatcga agacaatact ttaaaggcaa tctacgatgt ttctgtaaaa
     1201 acacttttag attgcatgca gaccgtcttt gaagatggtc ctaaacgaga tcgaagatta
     1261 tggttaggcg atctacgtct tcaagcacta gctaattact atacttttaa tgatttgtct
     1321 ttagtcaaac gatgcttgta tctttttggc ggaatgcgga caaatgaagg gaaaatacct
     1381 gctaatgttt ttacagaacc taagttaaca ccagatgata cgtttttgtt tgattatagc
     1441 ctcttttttg ttacttcctt atatgattat gttgcgcata ccaaagataa gaatgtctta
     1501 gcagatcttt atcttgttgc taaaaaggca attgatttag cactaaaaca agttgatatt
     1561 gaggggcgtt tggtactgac tgaagatttt cctgtctttg tcgattggtc gaatgatttt
     1621 gataaagcaa ctgctggaca agcaatcatg atctatacat tgaaacgatt tttaaaactc
     1681 gcgaaactat cgaatgaagc gaatgaaatg tactatcaag aaatactaga aaaaatgata
     1741 aacttttcaa accaaactct atttaatcat gagaaaaaac agtttgtcat tgaaaaaaac
     1801 aatgaaataa atgttgccag tcaagtttgg atggtgttag ccgaagtatt tgacaagcaa
     1861 acaaataagg aaatcatgct agaaacgatt aaatatcatt ttccaattca aggaattgcg
     1921 acaccttata tgtatcatca tgtggtggaa gctttattta tagccgattg tcaagaagag
     1981 gctataaaat tgatgaaaga atattgggga aagatgattg aattaggtgc agatacattt
     2041 tgggaagctt ttaaaccaga agatttaacc ttttctccat atggtagccc aattttaaat
     2101 agttattgtc atgcatggag ttgtacacca gcctatttaa tagggaaata tctgataggt
     2161 aaatagttag ttaattagga aaaatacatg gatcagttct tacttgttaa tgaattgatc
     2221 cgtgatttaa gaaaaaatga aagctgttat ctctgttggc agatatagta aaggaggctt
     2281 attatggaaa caacacaaat gagtctatta aaaagaaagt ggttgacaaa atggcgacaa
     2341 cggattggtt atggggtatc tgacttcgca tgtaatctta tctggcaagt aatttcgctc
     2401 tatctcttat atttttatac ggatatcatg aatttgaacg ctgcagcaat cagcctgatg
     2461 tttgtagtga cgcgttttgt agatggtgtg acagatttat tggttggcta cttgatcgat
     2521 cacacgaata cacgctgggg aaaatcacga ccgtattttc tttttggagc agttcctttt
     2581 gcactttttg cttttttgtg tttcaatgtg cctgattttt ctaattcagg aaaattagtg
     2641 tatgcatatg tcacatatct tggtttgtct tttgcatata cattagtaaa tgtaccaatg
     2701 gcatcgattt tgccaagttt gacaaatgat acagaagaaa gaacggcact atcaacttct
     2761 cgaaaatttt ttgctttcct aggtgcaacg attgttagtt catctgtttt gacacttgtt
     2821 gctgtttttg gtaagggaaa tgaacaatta ggcttcaaga ttgtcatggg aatgtttggc
     2881 gtaatcagct gtctactatt cttttttaca ttctccgctg ttcgagaatt gcctgtccat
     2941 ggaacaaaaa atccttcatt caaaagggtc tttacgtctt tgaagcaaaa taaaccttgg
     3001 atgatctttg cagtcaatat cctgtttatg tggaccagtt tttttattta atcaagtgca
     3061 ctagtttatt atttttctgt agtgattgga agcaagacac tttctattac tgtagcgacg
     3121 attatgtcaa ttgttcctat gtttgcaaac ttttttgttc ctatacttgc taaactatta
     3181 ggcaaaagaa atttatatct tttatccgca agtatccagt tattaggatt gatagttatc
     3241 atgtttgctg ctaagaatat aacactgata cttgttggta cctttatttc agccattggt
     3301 tacggagtaa aagaaagtat ttatttttcg atgcaagcag atccagttga ttttggtgtt
     3361 tggaagacag gtgtgaatgt ttctggttca ctttctgcaa ttaatggctt tttagggaaa
     3421 gttgcacagg caatttctgg tggtgtagcg ggtgccttac ttgcttgggg aatgtatcaa
     3481 cctggagaaa tcagacaatc cggaaaggca atttttgcca ttcaagcgat gtatctttat
     3541 gtaccaatgc tcttgattat ctgttcgata gtaacaatga tgttttatca tctagataat
     3601 atttatcccc aaataaaggc cgatttggaa atgaagaaat ctttggatgt caaatgataa
     3661 gaaaagtaac ttagatgaga atactaagta acaagaggtt gtgacttgtt tttagtcacg
     3721 acctcttttt tttatttatt ccatataaat acacattatt tattccgaaa agtgaaagcg
     3781 taaataacaa aaacaaccaa cttatttatt attaaagaaa agcgtattta agtaggacga
     3841 ttctgttgag agctttcgta gattttccca ttttcctatg gtaaactgac aaagttgaga
     3901 atgttagaac agatcgttta ggaggaaaca gatgtttacg aatgaaataa aaatcatgct
     3961 ttatgtggac gatgtagaaa aaaatgcaac gttttggaga gcagttggct ttgcagaaaa
     4021 agatcgtcaa gaaatggatg ggacgcttat aattgaagta ggagttgaag atagtcaaac
     4081 ctcatttctg ttatatgatc gagcatttgt tgagtcccat tcacctggca cagctactag
     4141 tgccccatct ttgatgtttt acagtaaaga tatctttgga ctatataaaa aaatgcagac
     4201 gcaatcagtc acattaggtg atatggtaca gcttggagaa gaatatgtct ttaactttgc
     4261 tgatcccgat ggcaactact ttgcggttac aggtaaataa tagaaataaa gactttgttt
     4321 ttctgtgcga aacaggaaag cgaagtcttt ttaattcaaa taataatatt atgtaaataa
     4381 aaaaactttc catgcgtaat tatacacaag caaataaatc gtcaaatgcg aacgccttct
     4441 ttatactgtt taggaacgaa aagaaggaag gaagaaatac atggattatc aagtactgct
     4501 ttattacaat tatacaaaaa tcgaagaacc agaggaattt gccaaaaatc atttagcctt
     4561 ttgcaaatct ttggatctta aaggtcgtat tctagtagct gaagaaggaa taaatggtac
     4621 tgtgtctgga accgttgaaa acacaactgc ctatatggaa gcaatgaaag ctgatcctcg
     4681 atttgctgat acgatgttca agatcgatcc aagcgaagga catgccttta agaagatgtt
     4741 tgtaagatac cgtccagagc ttgtaagttt aaagctagaa gaggacattg atccaaacca
     4801 agtgacaggt gcttatcttt caccgcaaga atttaaagaa gctattttag atgaacagac
     4861 agtggtgatt gatgcacgga atgattatga atatgacttg ggacattttc gaggggctgt
     4921 acgcccagat atacgcagtt ttcgagagct tccagactgg atccgcgagc acaaagaaga
     4981 atttatggaa aaacgggtag tgacctactg tactggtggc attcgttgcg agaagttttc
     5041 tggttggctt gtccgtgaag gattcaaaga tgtcggacag ctgcatggcg gtattgctac
     5101 ttatggtaag gatcctgaag tccaaggaga attatgggac ggcaagatgt atgtgtttga
     5161 tgaacggatc gctgtcgata tcaaccaagt agataaacaa atcgtcggtc gcgactggtt
     5221 tgacggaaca ccatgtgaac gatatatcaa ttgtagcaat cctgagtgca atcgtcagat
     5281 tcttgcttcc attgaaaatg aagaaaaaca tttaggagca tgtagcatag agtgtgcgaa
     5341 acatccgcgc aatcgctatg ttcaagacca tggaatctct ccagaagaag cagaactgcg
     5401 gtttacagct ttaagtaaat agaaaacaat agttttgatt gtcgaccatt tccaatatgc
     5461 gctatactag cgttgaggat ggtgatctat atgaaatcat tactttatgc tattggggat
     5521 gtccatggca aatatgagct ttttgaaaaa cttattcact attatgatcc aaagatccat
     5581 cagcttgttt tattagggga tctcaatgat cgaggtcctc gtacaaaaga atgtttttta
     5641 ttaggaaagc aattagtcga agaaacagga gccatttacc tccggggaaa ccatgaagaa
     5701 tattttttgc aatttctgca atcaccggag gactggtatg cgccttatat gagaaatggt
     5761 ggaaaagaaa cgtttgaaag cctgcttcac ccaggggcat cggaggaata ctccccaact
     5821 gaaatctcga tgatgatcca atcgagatat tcagaactga ttaaattttt agtccagcgc
     5881 cccttgtatt atgaatggga aaattatctt tttgtccatg ccggtgtgga tttgacaaag
     5941 aaagactggc gtaagacaag tctaagagac tttatttgga taagggaacc atttcatgaa
     6001 gggaaaaaca atacaggtaa gacaattgta tttggacata cgattacacc aatgctccat
     6061 ggagatatgc agacaacaga cctctggatt caggatcata aaatcggaat ggacggtggg
     6121 gctgtattcg gagggtcatt gcatggggtt gtttttgaca aggaaggtat tcttcaagat
     6181 attgaatatc agaacaacga aggaccttgg catggaatct gactaatgga aagaatgaag
     6241 gagctgtgac tttgttgcgg ctctttttat ttgtatagag aatattgttt ctttcagtag
     6301 tttaacccag gataaaaagt atgatagaat aaagaagcaa tttttagcag aaaagaggca
     6361 ttttaatgac ggaaaatttg aaccagacga tcattcagct tgtacaaaaa gagctttcag
     6421 actatcgtcc taagcagttg actactgtac ttaatttgtt aaatgaagga aacactgttc
     6481 cttttatcgc gcgttaccga aaagaaatga ctggaagtct tgatgaagtc cagattcgtg
     6541 aaatcgaaga gcggtatgca tatcttgaaa acttagaaaa acgtaaaact gaagtgattc
     6601 ggctaatcga tgaacaaggg aaactgactc ccgagttaga agcagaaatc acccaagccg
     6661 tgaaaatgca acaagtggaa gatttgtatc gtccatacaa acaaaaacga cggacaaaag
     6721 caacgattgc taaagaaaaa ggacttgaac cactcgcgaa gtggctgatg cagcttacag
     6781 atggagaggt gcaatctgaa gcagaaaaat atattgacaa agaaaaaaaa gtctcttctg
     6841 ccgaagaagc gttgcacggt gctcatgaga ttattgctga acaagtcagc gataatgcaa
     6901 aatttcggac ttggattcgg tcttatacgt ataacaaagg tatgtatgtc agcaatgtca
     6961 aggatgagca agcagatgaa aaaggcgtat atgaaatgta ttatgacttt gcagaacccg
     7021 tccataaaat ggtttctcat cgaatattag ctactaatcg tggagaaaaa gaagatattc
     7081 tgaaagtatt ccttcaagta gatgaagcag caattttagc ttacttggat cgccaaatag
     7141 tcaaaaatcc tgcctctcct tcttcttcat tcgtcagaga agcctatcag gatagttata
     7201 aacgctttat tcagccagca atcgagcgag aactgcgcaa tgaattgact gaaaaggcag
     7261 atgagcaagc aatcgctatt ttcggtgaaa acctccgtaa tcttttgttg caaccgccac
     7321 taaaaggtaa agttgtcctg ggatttgatc cggcttatcg tactggatgt aaactagcag
     7381 tcgtagatgc gacagggaaa gttttagcaa tcgaagttat ttatcctcat aaacctgcag
     7441 cgcaagcaaa aagagaagct gctggaccag cttttatcca attaattaat aaatatcaag
     7501 tagatatggt agctatcgga aatggtacag ccagtcggga atctgaactt tttgtggctg
     7561 agcagttgaa atccgccgac cataaaacct attacgctat cgtcaacgaa gcaggcgctt
     7621 ctgtttactc cgctagcgaa attgcacgaa aagaatttcc gcatctacaa gttgaagaaa
     7681 gaagtgctgt ttctattgcc cgtcgcctgc aagatccttt agctgaatta gtcaaaatcg
     7741 atcctaaagc tgtcggagta ggccagtacc aacatgacgt ttctcaaaaa cgtttggctg
     7801 aacaattgga ttttgttgtg gaaacagcgg ttaaccaagt aggtgtggat gtgaatacag
     7861 caagcccgca gctcttgcag catatatcag gattgaataa aacaactgcc caaaacattg
     7921 tcatttatcg tgaagaaaac ggagaattca ccgcacgaac tcaattgaaa aaagtgccac
     7981 gcttaggacc aaaagcatat gaacaagcaa tcggattctt gcgagtccct ggtgggaaaa
     8041 acattttgga caacacaggt atccatcccg aaagttattc tattgcaaaa gaaatattaa
     8101 cttcagtcca actttcagaa aaagagcttg gtacagaaga agcagtagaa aaattgacac
     8161 gattatccgt agagaaactg gctgaaagcc tatcagtcgg agaagaaacg ttaaccgata
     8221 ttttagctgg gctgacacaa cctggacgag atatgcgtgt tgagatgcca gcgcccttat
     8281 tacgtacaga tgtattgagt atggaagact tgaaaccagg gatggaattg actggaacgg
     8341 tccgtaatgt cattgacttc ggcgcatttg ttgatatcgg tgtgaagcaa gacggacttg
     8401 tccatatatc gaaactcagc aaaaaattcg tcaagcatcc tacagatgtc gtgtcagtag
     8461 gagatatcgt aacagtttgg atagaacaag tcgatacgaa aaaaggcaga atcagtttga
     8521 cgatgctttc cccttatgaa gagtaacaat gaggagtaac aaaaattgac acaagaagaa
     8581 ctgcagcagt tagtagaaga gatatcatta aaaagcttcc agcttccttt caagcaccaa
     8641 gcaatgttca atccacgctt acgtacaact ggcgggcgct atcatttaaa tgatcatcat
     8701 ttagatttta atgcgaagct atttgaagag tcagacgaag agacgattgc aggaatcatc
     8761 aagcatgaac tttgccatta tcatcttcat ctagctggaa gagggtatca gcataaagat
     8821 atggatttca agtatctttt gaaaaaaaca ggagggttgc gctatgcacc agccttgacg
     8881 aagaaacaga taaagatgga gtattatcga tgccaagatt gttctgccac gatctgccgc
     8941 aagagaagaa tcgatgtacg caaatatcgt tgtggaagat gtcatgggga aatcaaatgg
     9001 attgagacaa aaagagaaga ctttaatgaa gcacaaaaaa tcagatgaaa ggatggaagg
     9061 cctttggaaa aaagtgagca gaggaatcta aaagactgga atcagctatt aaatttttta
     9121 gccgcacctg aagagggaaa ttttgaggaa ctgacggcac ctacacttat tttagctgga
     9181 aactgtcttc ctatcttagc tgataaagca gcacaaatgt atttaaatgg ccaagtagat
     9241 caattgtttt tggtcggagg ggtggggcat gcgactagaa tcctttatga gaattttgaa
     9301 aagcaaggat ttcattttga agatggcatg agtgaaagtg aaatctgtcg tcagtatcta
     9361 aaagaagtct atgatttgcc cgataaggct tttctgatag aatcgaagtc tacaaattct
     9421 ggagagaacg ccactttttc actggagatt cttcattcct taggagctgt actagaaaaa
     9481 atcttattga tgaatgatcc gacactgcaa cgcaggactc aagcaacttt tgagaaagta
     9541 tggaaaagtg aacaaactat tttcgcgaat gctgtgccat ttgtgccgga aatccttcac
     9601 ttttctgaag agatcatctt tacagcaaaa gaactgaatc atcaatggcc taaggaatat
     9661 ttccatgcgc tagtattagg agaaatggaa cgactccatg acgatgagaa cggctatggt
     9721 cccaaaggaa aagattttat tcctcacatt gatatatcag aggaagtttg gtcttcttac
     9781 accagaatta aagagtcaat aaaaacggat ttttcccgta cttgaccatt ttagcaagcg
     9841 aatcctcctt tccaaataaa tttggttgaa attttcaatc gtttataaga taatgaggag
     9901 cgtgttgaat gtaatagcta aaagaatgga cgctagataa gagaagagga ttcaaatgga
     9961 aaatcaaaat aaattgtatc attttgttgg tatcaaaggt tcaggaatga gctccttggc
    10021 tcttgtttta catgaacaag gtctaaatgt ccaaggatca gacattgaaa aatatttctt
    10081 tacacaaaga gatttagaaa aagcaaatat cacgattctg ccatttaacg cagataatgt
    10141 aaaaccaggg atgacgatca ttgcaggaaa tgcatttcct gattcacatg aagaaatcca
    10201 gcgagcaaaa gaattaggtt tggaagtgat tcgctatcac gactttattg gtcattttat
    10261 ccaaaactat acgagtatcg ctgtaacagg ctctcacgga aaaacaagta cgactggact
    10321 gctctctcat gtattgagtg gtgtccgtcc aacaagttat ctaattggag atggaacagg
    10381 acatggtgat ccgcaagcgg aattcttttc atttgaggcc tgtgaatatc gccgtcattt
    10441 ccttgcctat tcaccagact atgcaatcat gaccaatatc gattttgatc atccggatta
    10501 ttatacaagt atcgacgatg tatttacagc tttccaaaca atggctggac aagtgaaaaa
    10561 agctattttt gcgtatggtg acgatgctta tttacgcaaa ttaaaagcag atgtgccgat
    10621 ttattattat ggtgtcacag aaaacgatga tatccaagca cgaaagatcg aacgcacaac
    10681 ttctggttct gcttttgatg tctatcatgg ggatgaattt gttgggcact tcactgtacc
    10741 agcattcggt aaacacaata tcttaaatgc tttaggtgtt attgcagtag cttatttcga
    10801 aaaactggat ctgaaagaag tggcagaaga aatgatgact ttcccaggag taaaacgtcg
    10861 attcagtgaa aagatcgtag cagacatgac cgtagtcgat gattatgcgc atcaccctgc
    10921 agagatcaaa gcaacgatcg atggcgcacg ccaaaaatat ccagataaag aaataattgc
    10981 agttttccaa cctcatacgt ttacgcgtac gattgcattg atggatgaat ttgcagaagc
    11041 acttgattta gctgataaag tttacttatg cgatattttt ggttcggctc gggaagaaca
    11101 aggaaacgtt aagatcgaag accttggagc gaaaatcaaa aaaggtggag aagtcatcaa
    11161 agaaaataat gtatctccat tgttagatta tcatgatgca gtagtaatct tcatgggtgc
    11221 tggagatgtt caaaaatttg aacaagcgta tgaaaaactt ttaagcagca cgacgaaaaa
    11281 tgtactgtaa tacaattact gcagtgtcct tataaaaata gaaagagggg gagtggcaaa
    11341 aatcttgcca agctcccttt tttgcaactt atgataaacg gcgttcaaaa tcttgcatct
    11401 gcggtaataa gtccagccaa atcaaaaaca tgagaagctg tttttcttgg ctgttcttat
    11461 tacttgatgc agggcagctt tttgacgacc tctgtaaact ataggaatct gttaaatcaa
    11521 gggagcagaa cttgcctttt acgctacttt tggtacaata atgtatgatt aaaaataaac
    11581 tggagggttt ctatcttgaa cataggtaag ccgagaaaac taggaaaaac aataaatgaa
    11641 atcgaggaag gcgactcgtt gtccttaaca gaatctattg aagataacca gcttttgctg
    11701 tatttgggat tgacgaatga tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa
    11761 acagaatatc aacagccaat cgttccatca gtgatgctga tggggatcat cacaagtgca
    11821 atttcaaagc atcttcctgg accaggatca catgttgtca atttttctgt caattttatt
    11881 gaaccggttt tccattatga aacactgact tttcagtttg aggtcataaa agtagataag
    11941 atgaaggatg tggtgacgat ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg
    12001 gatgctgtcg ttatggtcca accgccacaa gttcctgaat tggaaatagc aatcgaaagt
    12061 gaaggtgaat tgaatgaata atccaagtac atcaatgtca ggtatcaatc gtttttatgc
    12121 aaaagtttat ggatttttag gtatgggact tgcccttagt gcagtcactt cttacttggc
    12181 tctttgggta tttcctttgc aattagcaac ttttatcaat aattttccac taggctttgt
    12241 gggtctgtgg atcgttgaac tgattttagt aatggttcta ggcgtaaaag cacaaaagaa
    12301 tccgagctta gcaatcggtg gatttattgc ctactctctt ttgaatggca taacattagc
    12361 ggttactctt gcatactacg atattggaac agtaacgcaa gctttcttga cagctacttt
    12421 gatgttcatg gggatgtctg ttgtcggagt aacaacaaaa agagatctta gcggggttgg
    12481 aagagcagga caggcagctt taatcggagt gatcattgca ctagtcttga acatcttcat
    12541 tttgagaagt agcgcagttg agctgttcat ctccctactg ttagtcgtca tctttgcagg
    12601 gatcacagct tatgataacc aaagcatccg taatatctat gtccagacaa acggaacagc
    12661 acatagtgga attgctgtat tcatggcatt gaagctgtat ttggacttta tcaacttgtt
    12721 cttggcgttt ctgagaatct ttggacggaa caactaaaaa agaaaaaaat tcatcagttg
    12781 gggctatacc gaattatttg gtatggtccc gctttttctt ttcgcattcc gcaggattct
    12841 ttgttagaat gaataagaac tttacagtaa ggagttaaga agatgacaaa aaataaatta
    12901 ttactcgttg atgggaacag tgtggctttt cgcgcattct ttgcgcttca caattctctt
    12961 gaacgtttca aaaatagaaa cggtcttcat acaaatgcaa tctatgcatt caatacgatg
    13021 tttgaaaatg ttatgcaaaa agagcaaccg actcatgttc tggttgcgtt cgatgcagga
    13081 aaaacgactt ttcggacaga aatgtatgcg gagtacaaag gtggaagatc caaaacacct
    13141 ggcgaattca aagaacaaat gccatatata cgtgatttgt tgacaggact aggggtacag
    13201 tattacgaat tgcccaatta tgaagcagat gatattatcg gtactttggc agaaaaagta
    13261 gacaaagatc aatttgatgt cgtcgtgctt tcaggtgatc gggatttgac tcaattagct
    13321 agtaaagaag taaaagtgga tatcactgta aaaggtgtca gcgatatcga aagctataca
    13381 ccagaacatg tagcggaaaa atatgatggg ttgacgccaa aacagattat tgacatgaag
    13441 ggactagccg gagatgcctc tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca
    13501 gctatcaagc tattgaaaca atatggctca gtcgaaggaa tttatgaaca tatcgatgaa
    13561 atgaagcaaa gcaaaatgaa agaaaatctg ataaatgaca aagagcaggc tttcttatcg
    13621 aaaaaattgg cgaccatcaa tacttcagcg cctgtggatg tctctatcga ctcattgaag
    13681 tatgaaggga aaaatctcga taaactcgtt ccgttttata aagaaatgga tttcaatcag
    13741 ttcctttcta aattgaatat cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa
    13801 gtcgttcatg aattcaaaga ggaaatgttt actacggata tggcgttgta cgtggaaatg
    13861 atgggcgaca attatcatac agaggaaatc gtcggagtag cgtgggggac agaaaagaaa
    13921 atttatgtca cgaacgatct atctgtattc gaaaaccgag cgttccattc ttggatcact
    13981 gatccgactc ggctgaaaaa agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc
    14041 tatactggaa aaacgaaagg aatcgatttt gatgtcttgc taggtgcata ccttctcgat
    14101 acgaatgaaa aaagcacaga tatcgaagga atagctgctc attacggata caacgatatc
    14161 caatcagatg aagctgtata cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa
    14221 ctcttttttg ctcatttagc gcgaaaagta gctgcaatca atgcgttgac accaaaattg
    14281 agccaagaac tggcagataa aaaccaagaa gatcttttcc ggaaaatgga actgccattg
    14341 tctatcattt tggcagagat ggaaataagc gggatcaaag tagatgcgac tcgcttacaa
    14401 gaaatgaagg gcgagttctc tgctcgctta agagagatcg aacaaaaaat ctacgaagaa
    14461 gccggcgaag aattcaattt gaattcacca aagcaattag gagtgatctt atttgaaaaa
    14521 atgggattac ccgttatcaa aaaaacaaaa actggatatt ccacagccgt agatgtactg
    14581 gaacagcttc gtgaacaagc accaatcgta gaggatatcc tcacctatcg tcaaattgca
    14641 aaaattcaat ctacctatgt cgagggactg ctgaaagtga tccaatcaga tggaaaagtg
    14701 catacccgtt atgtccagac attgacccaa acaggacgat taagctctgt tgatcctaat
    14761 ttgcagaaca tcccgattcg attagatgaa gggagaaaga tacgtcaggc atttgttcct
    14821 agagaaaaag actggctgat ttattcttct gactattcac aaatcgagtt acgcgtactt
    14881 gcgcatatct caaatgatga acatttgaaa gctgccttcc tagaaggtca agatatccat
    14941 gccagcacag cgatgcgtgt atttggaatc gaaaaggcag aggatgtcac accaaatatg
    15001 cgtaggcagg caaaagcagt gaattttggt atcgtgtatg gcatctctga ttacggactt
    15061 tcacaaaatc taggaatcag ccgaaaagca gcacagcaat acatcgatac gtactttgaa
    15121 aaatacccag gtgtcaaaga atatatggaa agaatcgtac gcgaagcaaa agaccaaggc
    15181 tacgtcgaaa cgctgtatca ccgccgtcga tatctgccag acatcaattc aagaaattac
    15241 aatatccgtt cttttgcgga acgaacagct atcaatacac caatccaagg aagtgctgct
    15301 gatattttga aaatcgcgat gatcgaattg gataaacgat taaaagaaac aggtcttcaa
    15361 gctacgatgc tgttacaagt tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt
    15421 gaatcattag ataaattggt gaaggaagtt atggaacaag ctgtttctct tcatgtacca
    15481 ctgatcactg acagcagttg gggaaaaacg tggtacgaag caaaataaac attgtttaag
    15541 acagagcaat ataatgaagg tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg
    15601 ttcgtaaagg attggaacgg ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt
    15661 ggcccaagat catcgagcaa ccagagacac ccatctttga ggcttctctt gttggcgaaa
    15721 ccatccaatc cattggacgt agagggaagt ttttaatttt tcatctcgat cattgtgaac
    15781 tgatctcaca cttgcgaatg gaaggaaaat atcaatttac gaaagaaaat acaccaatcg
    15841 ataaacatac gcatgtactt ttcttttttg aggatggaag ccaacttcgc tacaacgatg
    15901 tacgaaaatt tggtcgtatg acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa
    15961 tcatgaaatt gggacctgag cctttgccag atagcttctt attagcagat ttcgcaaatg
    16021 gactgaaaaa gtcccacaaa gcaatcaagc cattgctgct agaccaacgt ctagtgactg
    16081 gtctaggtaa tatttatgta gatgaagcat tatgggaagc gaagatccat ccagaacaac
    16141 cggcgaacac actgcgcccc aaagaggtag aacaattacg tctatcgatc attgatgttt
    16201 tggatcgtgc aatagaagcc ggaggtacca cgattcgcag ttatttgaat gcattaggag
    16261 aatcaggcgg atttcaagta gcactgcatg tctatcagca aactggcaaa ccatgtatac
    16321 gttgtggaac gccgatcgtg aaaacaaaag tggcacaaag ggggacgcac tattgtccga
    16381 attgtcaaag gctaaaaatc gcaaagtagg atttgtttta ggtttgacgg gtggaatagc
    16441 taccggtaaa agtacggcag ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg
    16501 cgatgtgatt gccagagaaa ctgttgagcc tggaacgctt gctctgagga aaattgtatc
    16561 agtgtttggc caagaggtat tgcagcctga tgggcaacta gatcgaggga aacttgggat
    16621 gattgttttt ccatcaaagg aaaaacggca aaaacttgat cagctattag atccgtttat
    16681 ccgaaaagcg ataaaagatc aaatcgcctc tctaagttcc aatcatcctt tagtaattgt
    16741 agatatccca ttgctctacg aaggtcatta tgaccactat atggatgcag tagctgtcgt
    16801 ttatactacg ccagaaactc agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga
    16861 acaagcgcag cagagaatta gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga
    16921 tattttgttc gacaacaatg gaacaaaaga aaaacttgtg ggacaaatcg aaaattggct
    16981 ttttgctaat cgctttcttt gagtatctgt gtcaattttg ctatcctgag tatatagaaa
    17041 gtgagggata gcgatgggat cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa
    17101 agaaaaaatc ggcgagatgc tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc
    17161 agaaacagca gaagaaaaag aaaagcagta ccaagcaaga atcgatgaaa tagaagacca
    17221 gcaggcagaa gaagaagcga agcggcaaga tttgactcaa gaacgaaaaa atccgacacc
    17281 tgatcgtagt aaaacaccgc tcccagaaag tgaccacatt cccgatgagc aaaaaatcga
    17341 ggaacagcga atgaaacact atacaggaat cgaagagaag ttataaaata gtaaaaaatc
    17401 gagatgtgac ttgaagcaga tgagaaggtg ttcatctgct tcaagttttt tgtgctagat
    17461 gactaaatca gcttttgtgt aagttatttc tcagtaatca aggtgatcag tattggaaaa
    17521 ttgaaattta gtaaggacag gggacacaat tacgcatgaa agtagaaaaa attgttctta
    17581 tatccgtgtt ttttgggtag gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac
    17641 tagttgtaaa gaaagacaca tcctttatat aaaatgcgat gcaagaggtg agacaaaatg
    17701 agatgtccca gatgtcaaca taataattca cgagtaatcg acagccgtca agcagatgat
    17761 ggacgtgcga tccgtcgccg tcgcgaatgt gagaattgtt cgttccgatt cactacattt
    17821 gaacgaattg aagctgctcc gttattagta atcaagaaaa acggggaacg cgaagagttc
    17881 aatcgcgata aaattttaag aggactcatc cgttcagcag aaaaaagacc tgtagctatg
    17941 gaacaaatgg aacaaatcgt caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac
    18001 gaagtatcga cgaatctaat tggtgaatat gtgatggaag atttagttga tttagacgaa
    18061 atcgcttata ttcgttttgc aagtgtttat cgccagttca aagatatgtc tgttttctta
    18121 aaagagttgc aagatatcgt ggataaagca aaagaaaata ataaagagaa taataaagaa
    18181 aacaattaag aaacaaaaag gaggcatttc cttggaaaat gcttggaaag agcttcaacc
    18241 aaaaaatatt taccaaggaa gaaaagcggc tccgctgaga gaagacagca agaacgcctt
    18301 gctgtatctt tatcagccac taatcggcgg agaagccttg agtatctatt tcaccctgtt
    18361 atcagaaatc tctcaagaat ctggcaaagg tcctgaaggg atgcatgcag atctgcttag
    18421 tagtctaggc tgcggtatcc cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg
    18481 cttactggat gtttatttta aagaagatag tgatctggga cctcgtttta tttatgagct
    18541 gttagaacca ctcattccta aagcattttt caaagactct gttctggctt ttcttcttct
    18601 ggaaaaagtt ggtgaacgac gattccagaa gcttacccaa ttctttgaac ctaaaaggca
    18661 tacagcagag ggatatcaaa aaatcacaaa aaaattttta gatgtttatc gcttcaatga
    18721 agcagtgtac gctgctaatc aagaaaacat cgaatcgctg aataaatctt ttgaagaatc
    18781 tgaatcagta gcattacttg aagcagattc gacattcgat tggcagtttt tgaatgattg
    18841 gctggcaaaa aaacatattg caagtcttga tgatccaatc atcaaacaag tcgacatgta
    18901 tcatcagctt tacggtttcg atgaattgac acttggcgaa ctgatcagtc aatcttatga
    18961 ctttagtgaa caaaaagtat ctgaaaaaga actgcaaaaa gccattttgg caatgcagag
    19021 acgtccggtg aagcaaacaa aagaaacagt tgcggtttct gaaaacagta atgaaccctc
    19081 gtcaaacgaa catttgacaa acgcagcgct tcagctgata aaagaagcac agagcattcc
    19141 accgatgaaa tatctggaag caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga
    19201 aaactggtta ctgcaagatc ttgtttctca atcaggtctt tccagtagcg tgatcaatgt
    19261 tttgctgaat tatgtactag tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa
    19321 tacagtggca aatgaatggg cacaaaagaa aatcgccaca gcagaagaag cgatccagca
    19381 cattcggcaa ataagtaatc aagcaaaaca atcaaaacaa aagaagcaaa caaattatca
    19441 aactggcaag cgaaatgttc gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga
    19501 tgaaaaacag atcagcagcg agaaaaaagc agagattgac cgccgtttta aagaatacct
    19561 ggcaaaaaag gagggtgata tctagtggaa gacgtaggca aagaattgaa gaggatcatc
    19621 agcaaacgca attatcaaga aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg
    19681 gatgttcaag cttttctaag cgcacaccaa gaagaattga cacaagcgga tattgaaaaa
    19741 agttacgcta aattgtatga attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca
    19801 acgatgatcg ctcccggcta tgagccgaag ctgacgttga attttcatta tgtggatgtc
    19861 acttatgtac caacggaagc attgttagca cggcaaagac aagaagaaat cagaagccga
    19921 gtaaaagcaa tggatatgcc aaaagatatt caatcggctt cttttgaaaa ctttgaacgt
    19981 acagatggac gaggatatgc gagcttagaa gccctagatt ttgtagagaa atatactgct
    20041 gatccaaaag ggtatcataa aggactgtat ctagtaggaa gttttggtat tggaaaaaca
    20101 tatttattag gagcaacagc gagagaatta gcgattgctg gattcaccac gacgctagtc
    20161 cattttccga catttgccgt tgagatgaaa caggcaatcg gaaaagatca agtggcagaa
    20221 aagttagatg cagtgaaacg ttcacctatt ttgatgatcg atgacattgg tgcagatgcg
    20281 atgagtagtt ggatacgaga cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa
    20341 cagttaccaa ccttcttctc atcgaatttt acaatggatg aattggaaca gcatttgtcc
    20401 gtcactcaac gtggagatga agagccgcta aaagcgaaac ggataatgga acgcatacgt
    20461 tatttgacaa aagaaatcga aatgactggt cgcaaccgac gcaatggttg aacagttgac
    20521 aagtccaact gacatttgac ctagtagtaa aagcaggtca gcgaatcatt atatggacag
    20581 aaatgaccgg agacaatcac cgcaacaagt aatgcagaaa cttaaacgga ggatttttta
    20641 tgagggctat tttttctagt atatctgtag ttggtctatt ttttctttta gcaggctgcg
    20701 gtagcgcaga tagcaacaca ggttctgttg attcgattga tgcgggtcaa acagtaaaaa
    20761 caagcagtac gaaaacggag agacaaagca tatttgtcgg aacgattgaa agtatcactc
    20821 aagtagaagc agaagaagaa acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag
    20881 atccagcgaa tatgggaacg tcattcagca atgacggagt gatcttaaat gctactgctg
    20941 aaaaaataag cgtggacata aatgaattga aaatcggtga caaagtccaa tttacattag
    21001 ttgagatgcc gatcatgacg atgtctatcc caccgcaagt tcctggagat tcgattattg
    21061 atgtttcatt gaacaaataa ccaataacat tttttcaaaa ataagaaaat gttgttttat
    21121 agaacgaaaa ggtatatttt ttaaggagat atgggaattt ttttattcct agtctcctta
    21181 ttttatttgg aaaatgctat aattatctag aaggagtgaa gctatgaacc atttagattt
    21241 atctcagcgc ttagccgcag tgggtgactt tgtccctgaa tccgctcgtt tagcagacat
    21301 tggatcagat cacgcttact tacccgttgc attgatatta agaggaaaaa tcgaatatgc
    21361 agttgctggt gaagtcgttc aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga
    21421 tggattagaa gaccgtatca ccgtacgcct tgcaaatggt ctagatgcta tccagccaca
    21481 agatggtatt acagctgtca cgattgcggg tatgggaggt tcactgatcc gagatatcct
    21541 agaagctggc aagttgaatc aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa
    21601 catcggagag cgtacattac gatcttggct gcaagaaaat cagtataaaa tccaatcaga
    21661 aaaaatactt gaagaaaata aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa
    21721 accagttact tacaatgaaa tggagctgat gttcgggcca tttctgcttg aagagaaaag
    21781 tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca
    21841 gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat
    21901 gattgaggag gttttaaagc catgacaatc agcggacgca ctttcatcca aaagtttgaa
    21961 agctattgtc ctcagtggct agctgaagaa ggagacccag tcggactgca tatcggaaca
    22021 ttggataagc caatccaacg agtaatgatg acattagacg ttcgtccaga agtggtcgaa
    22081 gaagcaatca agaaaaaaat cgatttgctg attgctaaac atcctccaat ctttcgtcct
    22141 gtaaaacggc tggttactga tcagccacaa gaaaaaatgt atgcagattt gttgaaacat
    22201 gatattgccg tctatgcagc acacacgaac atggatatta tttgggatgg actgaatgac
    22261 tggttttgtg aacttctcgg tattgaagtg gagagttatt tagtaaagac tcatgagatt
    22321 cattataaaa aactggccgt ttatgtacct gtggatcatg cacagaaaat gcgtgaagta
    22381 ttagctgcag ctggtgctgg tacacaaggt gattataccg gtacaagttt tacctcgatt
    22441 ggacacggtc gatttactcc ggaagctggt gcacaaccag cgattgggaa agtagggaag
    22501 acagaacaag ttcaagaggc aaaagttgag gttattttgc cggaaactat cgaaaaacaa
    22561 gtgattcaag ccatgcggtc agctcatcct tatgaggaac ctgcttatga tcttttcgct
    22621 atagatgaac cagtcgaaat gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt
    22681 tctatagagg catttgtaga acaagtaaaa gaagcgttcc aactagatgg actgagaata
    22741 gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg cgatttgcgg tggaagcggt
    22801 gaaaaatttt atccgcaagc aatcgcccaa agagcagatg tctatatcac gggagatatc
    22861 tattatcata cggctcatga tatgcaaagc gctgggttga ttgcaattga tcccggtcat
    22921 tatattgaat cgttatgcaa gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa
    22981 gaaaattggg atattaactt tttcgtttct gaaacaaata caaacccatt tcaatttaat
    23041 taaaaggaga gaatactatg tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa
    23101 cagaaacacg ctccgatgca acaagcaaaa cgactccatc gactcagaca caagttgctt
    23161 ttgcgcaaga attgaaaaaa gaattagaag agttaggctt agctgatgtt ttctataatg
    23221 aagataacgg attcgttatg ggaacattac caagcaatgt tgatcatgaa gtgcgttcca
    23281 ttggatttat ttcccacatg gatacagcag attttaatgc agtaggagtc aatccacaaa
    23341 tcattgaaaa ctatgatggc gagtctacga tcgtactaga tgcggaagaa cgctatacat
    23401 tgaatacaaa agattttcca aatttgaaaa attatgaagg ccacacattg atcacaacag
    23461 atggcagtac attactggga gcagatgaca aatcagggat tgcggaaatc atgactgcaa
    23521 tggagatctt gatcaataat ccttctatcc ctcacggtac catcaaagtg gcttttggtc
    23581 ctgatgaaga aatcggtgtc ggagcggata aattcgatgt tgagcaattc gatgtagatt
    23641 ttgcttatac agtcgatggt ggtcctttag gtgagttgca gtatgaaacg tttagtgctg
    23701 ctcaagcaaa tattacgatc caagggaaaa atgtacatcc agggacggca aaagatacaa
    23761 tgatcaatgc tctacagtta gcaatcgatt ttcataatca gcttccagcc gatgaagttc
    23821 cagaaaaaac ggaaggatac gaaggtttct tccatttgat ggcattggat gggaatcctg
    23881 aagaagcttc tatgagttat atcattcgcg atcatgatcg tgaaaaattc gaagcacgca
    23941 aagcacaaat cacacatatc caagaagagt tgaatcagcg atttgatcaa gaacgaatca
    24001 aagtagatat gtatgatcaa tattacaaca tgaaagaaat cattgaaaaa gatatgagca
    24061 tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat ctctccagtg atcgaaccag
    24121 ttcgtggagg aacagatggt tcaaaaatct cttacatggg tattccgacg ccaaatctat
    24181 ttgccggagg agaaaatatg cacggacgat tcgaatttgt ttctttacaa gtaatggaaa
    24241 aagcaacaga cgtgattgta aaaattgcag aattgaacac aaagagttaa aatcgaaaaa
    24301 gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag
    24361 ggcaggacaa tggaattggt tgtcccgccc tcttttttga agagtaaata ggaggtaaag
    24421 aatggaaaag ctgctagttt tttataacaa aagttcaggg aaagatgagg gtgagaaatt
    24481 agcacgctgg tttcaagatt atgtaaaaga aaagcagcca gaattatatg tagaactagc
    24541 tgaaactggc ccatcgattg atgatgcgga attagtcaag aaagcagaag ccatagaagc
    24601 tgatacttta gtaattatag gtggagacgg cacgattcat catatcgtcc aagcgttcca
    24661 aaaaacatta tctcaatacc atgtcggatt acttccaggt ggaactgtca acaaccttgc
    24721 acgtgttctt gagattcccc tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa
    24781 tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc attatctcta cactgacaat
    24841 cggaatctta gcagatacag cagtttggat cagtcaagaa gaaaaacagc gttacggctc
    24901 gtggatcttt atcaagaagt tttttaagtt attgctgaag aagaaaaaat atcatttgga
    24961 tttagaaaca gacgaggagc attggaaagg aaaatcccaa ctgttgacag tcacgatgtc
    25021 taactccgtg ggcggtttta caaattttga cgactcagcg accctagatg atggaaaatt
    25081 tcatgtaacg atcgtacctt ccttagatat tttccgattt attttttatt tgcctagaat
    25141 catgagaggc aaattctata cgatccccgg cattacttat ttcacagcca aacgcatgaa
    25201 aatccaagcg caggaaaaat ctgtgggcac tcgaacggat ggagacacga cagatgatct
    25261 tccagttgaa ttatctgttg tatcaaaagg attaacagtt tattcaccaa agaaagaaga
    25321 aaaagcttag aaatagtagt taacagaaag ttggaagaag agtaatggat gtattgttac
    25381 acaatccttt gtggttttac cttttcattg tgaattttta cttattttgt ctgatggggt
    25441 atgataaaca tcaggctaga actagcggct ggcgtgttcc tgagaataat ttgatatttg
    25501 tcagcctgtt aggcggtggt ttgggaggat tgatctctcg aaaagttttc catcataaaa
    25561 ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt acttgttgat ttgattctca
    25621 tttatttttt ccattaagga gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt
    25681 ctgaatctgc ttttgctgat tgtgattttt gccattattt attatttaat caatcaatca
    25741 tttgcagata tctttgccga attgctgtct accagtgttg ttgttttggc agcggctgtt
    25801 atattaggga cagtttacca aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct
    25861 ttcgcgccga attttacaac gaaagatggg ttcttgacct ctagctatat tgcgttttac
    25921 cgaatcgttt cgtttggtac aggaacgctg atctcggaga tttattttta taacaaaaaa
    25981 ggaatgaagc tatcccaggg agcaggggtg acagcactcc atatgattat gtacaaactg
    26041 gcagtgatca cttatgcatt agctggcttg gtcatccaat tctctctttt ttattctaat
    26101 ggtccatcaa tgatctggtt tatcatcgct ggtatccttt tgacgatcgt gattgttgcc
    26161 tttttattgg cgatgtccgt cagcttgaat gttcaaatat tttttgtcag cttatcgaat
    26221 aagttattta aaagtgagcg tttgcggaaa tgggtggata catgcaatca gcagatctac
    26281 tctttaaggg aaacggtcca aaccattatc caagacagga ctgcgctatt gcggatttat
    26341 ctatggaata tgttaaaact tgtattttgg tatgtacttc cttatttgat tctgattgaa
    26401 aaccatccta aaatagattt tctactgacc atttcgttta tcagctttgc agtagtgctt
    26461 tcaggggtta ttcctacgcc tgctggtatt ggttcctttg aatttgtcta tttgttatta
    26521 ttcagaccag ttgttggaac agttgacgca gtttcttcgc ttttactgta tcgtttcagt
    26581 tcctttatct tgccatttct ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg
    26641 atcaaagaag aaataataga agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt
    26701 tataagatct acccttttta tcaaatcaaa gaaaaacgtt atggataaga taattttacg
    26761 tttgtttggc tcttttttta tatacataca atcaattttg tttttttaag atttatataa
    26821 gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac caaaattgac caaagcgata
    26881 taatagtttt tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac
    26941 ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca
    27001 gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt
    27061 acaacctaat cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt
    27121 tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca
    27181 atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg
    27241 tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt
    27301 aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg
    27361 taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca
    27421 atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat
    27481 ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa
    27541 aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga
    27601 agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat
    27661 tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga
    27721 aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat
    27781 cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa
    27841 tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga
    27901 tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt
    27961 agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt
    28021 tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc
    28081 tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc
    28141 cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag
    28201 acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag
    28261 taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc
    28321 gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc
    28381 tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg
    28441 cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga
    28501 agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat
    28561 tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg
    28621 agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc
    28681 agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg
    28741 tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa
    28801 agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga
    28861 atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta
    28921 cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt
    28981 ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga
    29041 cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat
    29101 gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc
    29161 agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt
    29221 gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat
    29281 cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac
    29341 aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc
    29401 aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt
    29461 tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca
    29521 tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa
    29581 aaggggtctg acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag
    29641 tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct
    29701 tttattaggt atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt
    29761 tactttgaga ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt
    29821 cgcccatcgt atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa
    29881 aacgttgaag attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca
    29941 cgccgcaaag tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg
    30001 atatctcagc atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca
    30061 ttggactcac cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc
    30121 ctgtgcctaa aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg
    30181 gtattttaag tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta
    30241 ataaagattc cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt
    30301 cataaaaatt atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt
    30361 tgatttcttt aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac
    30421 tcaatttctg aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc
    30481 aggtagtgaa attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt
    30541 ccgccttgct gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc
    30601 acaccgattt ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt
    30661 aaatcactag ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa
    30721 gcagttttgt cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag
    30781 agctctctga tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt
    30841 ggtgcggcaa aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa
    30901 gtaagcttcc atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc
    30961 taaccgttcg attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa
    31021 tgtttggata tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc
    31081 ctgttcatcc catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat
    31141 gatgaaatga aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac
    31201 atcaacgatc cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat
    31261 caaaaatgct aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa
    31321 atgtgcggaa ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc
    31381 ttttacgtga aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt
    31441 gctatcgtag tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc
    31501 ccaatgaaaa tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac
    31561 ggaaaatacg tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg
    31621 tttaggtagc tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc
    31681 tttacgccga tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt
    31741 gcgcatatta tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc
    31801 tggataagtt ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac
    31861 ttcacaagga tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac
    31921 tttcgatact tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc
    31981 gctttcctcg atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg
    32041 atcgatatgt tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc
    32101 cattttttca tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac
    32161 ttctatagct gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa
    32221 gtgctgatta tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag
    32281 agcagattcg taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg
    32341 atcgtatttt agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag
    32401 actcgatgga tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca
    32461 attacacagc gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg
    32521 tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg
    32581 tcgatgcagc acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca
    32641 atgatttgga gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac
    32701 gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca
    32761 gtgccaaatc agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg
    32821 caccaagtgg agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata
    32881 gttaccgcgg tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca
    32941 aaatcaaact gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc
    33001 caaaagcagt tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta
    33061 tcaaaactgt ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg
    33121 ctgaagcttt accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa
    33181 ttgatacttc tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg
    33241 ctgctttaca attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat
    33301 tcctcggact tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag
    33361 aattgatcac gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg
    33421 ctacagtaga taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac
    33481 cattcgtcaa agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt
    33541 ctcaacgaaa acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg
    33601 tcgtctataa tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa
    33661 gcacgaaagg ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag
    33721 tgaagatgga catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc
    33781 gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac
    33841 gccaacaatt tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag
    33901 acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta
    33961 aacgtaaact cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg
    34021 tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga
    34081 aatagcaagg gtttgtttga aaacagtgta aaaaataaac catgtttaca tggatttgcc
    34141 accggtttgc caccggtggt ttttttcggt ggcaaaaatc aagaattctt tctaagattt
    34201 tccatgatat cgaccgtttc atttctcatc ttatctgtta catgcgagta agtatccatc
    34261 gtaattgaaa ttctgctgtg gcccaatcgt tcagagattt ctttcatttt tgcgccattt
    34321 tcgagaagaa gtgtagcatg agtatgtctg agagaatgga agttaaaaga gagggagagt
    34381 gcattcgata tcctcctagt attccatttc actacacttg gcgtaactag ctcaccgtct
    34441 tccttcgtac atactgcatt tgaatcaatg tagagctttc catacttcat tcgattttct
    34501 aattgttgtt tcttatgttt tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt
    34561 cgattggaac tgcttgtctt tggcgtacca tatacccatg cgccgtcatt ctttaccatt
    34621 tgtttctcta cagtaattgt tccatttgaa aaatcgacat catcccacgt cagaccacaa
    34681 acttcgccaa cgcgcattcc cgtataaaat ccaatattca aaggaatata gaaaggatgt
    34741 ccttcaggag taatttctag catatgatca aagtcctcaa gagaaatgat ttttagatct
    34801 tttttagtcg ttggtcgtgc ttcatatttt ggtattttta catacagcat aggattttgc
    34861 ttgattaatc cccaaggata aaccgccata ttcagcgcat tcttaaggac agagtgagta
    34921 atagtcattg ttttcttcga gtaacccttt ttaaattcag cattgatgaa attttgtaaa
    34981 agagcagggg agagatccgt aagttttttc tttcctaaat aaccgtttat atgatttttg
    35041 atggtaaatc ggtagttttc ataggtattg tattttagat ttagtttaac gtattcctcc
    35101 atccaaaaat caaggtattg ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt
    35161 tcgtcaatat ctgataaaac ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg
    35221 ccaactttct ccactttttt tcttgagcca tcatcattga tatcttcgcc gtttgtataa
    35281 ttaagcgaga caaataaaaa agccatttat gttacactca aattgtaata cccgctaaag
    35341 catacaa
//
LOCUS       DANKHK010000017        34864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000017 DANKHK010000000
VERSION     DANKHK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            47..799
                     /locus_tag="PW109_000769"
     CDS             47..799
                     /locus_tag="PW109_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2510638.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            796..1710
                     /gene="pfkB"
                     /locus_tag="PW109_000770"
     CDS             796..1710
                     /gene="pfkB"
                     /locus_tag="PW109_000770"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2510639.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1728..3659
                     /locus_tag="PW109_000771"
     CDS             1728..3659
                     /locus_tag="PW109_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2510640.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3770..5329)
                     /locus_tag="PW109_000772"
     CDS             complement(3770..5329)
                     /locus_tag="PW109_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2510641.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5484..7718
                     /gene="pcrA"
                     /locus_tag="PW109_000773"
     CDS             5484..7718
                     /gene="pcrA"
                     /locus_tag="PW109_000773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2510642.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7735..9771
                     /gene="ligA"
                     /locus_tag="PW109_000774"
     CDS             7735..9771
                     /gene="ligA"
                     /locus_tag="PW109_000774"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2510643.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9877..10182
                     /gene="gatC"
                     /locus_tag="PW109_000775"
     CDS             9877..10182
                     /gene="gatC"
                     /locus_tag="PW109_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2510644.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10182..11654
                     /gene="gatA"
                     /locus_tag="PW109_000776"
     CDS             10182..11654
                     /gene="gatA"
                     /locus_tag="PW109_000776"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2510645.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11651..13081
                     /gene="gatB"
                     /locus_tag="PW109_000777"
     CDS             11651..13081
                     /gene="gatB"
                     /locus_tag="PW109_000777"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2510646.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13091..14146
                     /locus_tag="PW109_000778"
     CDS             13091..14146
                     /locus_tag="PW109_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2510647.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEARRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRVIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14257..15630
                     /gene="rlmD"
                     /locus_tag="PW109_000779"
     CDS             14257..15630
                     /gene="rlmD"
                     /locus_tag="PW109_000779"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2510648.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRTGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGVKPSVIVVDPPRKGL
                     DHRFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15633..15761
                     /locus_tag="PW109_000780"
     CDS             15633..15761
                     /locus_tag="PW109_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2510649.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16202..16390
                     /locus_tag="PW109_000781"
                     /pseudo
     CDS             16202..16390
                     /locus_tag="PW109_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            16468..17481
                     /locus_tag="PW109_000782"
     CDS             16468..17481
                     /locus_tag="PW109_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2510650.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17548..18582)
                     /locus_tag="PW109_000783"
     CDS             complement(17548..18582)
                     /locus_tag="PW109_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2510651.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18696..20921
                     /locus_tag="PW109_000784"
     CDS             18696..20921
                     /locus_tag="PW109_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2510652.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALIHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHSDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLITDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20939..22822
                     /locus_tag="PW109_000785"
     CDS             20939..22822
                     /locus_tag="PW109_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2510653.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGTWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSTAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGYFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VYLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPEGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22803..23777
                     /locus_tag="PW109_000786"
     CDS             22803..23777
                     /locus_tag="PW109_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2510654.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            24436..25458
                     /gene="aroF"
                     /locus_tag="PW109_000787"
     CDS             24436..25458
                     /gene="aroF"
                     /locus_tag="PW109_000787"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2510655.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGTQSLNEKTYSRMMKEVAIMKEAME
                     KIQRLD"
     gene            25491..26555
                     /gene="aroB"
                     /locus_tag="PW109_000788"
     CDS             25491..26555
                     /gene="aroB"
                     /locus_tag="PW109_000788"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2510656.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            26557..27723
                     /gene="aroC"
                     /locus_tag="PW109_000789"
     CDS             26557..27723
                     /gene="aroC"
                     /locus_tag="PW109_000789"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2510657.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRAAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSQVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSIDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            27740..28831
                     /locus_tag="PW109_000790"
     CDS             27740..28831
                     /locus_tag="PW109_000790"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2510658.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERTNLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            28844..30139
                     /gene="aroA"
                     /locus_tag="PW109_000791"
     CDS             28844..30139
                     /gene="aroA"
                     /locus_tag="PW109_000791"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2510659.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     THTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLQGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            30132..30641
                     /locus_tag="PW109_000792"
     CDS             30132..30641
                     /locus_tag="PW109_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2510660.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            30638..31465
                     /gene="pheA"
                     /locus_tag="PW109_000793"
     CDS             30638..31465
                     /gene="pheA"
                     /locus_tag="PW109_000793"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2510661.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIEITSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINLSKI
                     ESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEETRR"
     gene            complement(31528..33264)
                     /locus_tag="PW109_000794"
     CDS             complement(31528..33264)
                     /locus_tag="PW109_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2510662.1"
                     /translation="MRKKIKEHELFTKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVESYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(33261..34769)
                     /locus_tag="PW109_000795"
                     /pseudo
     CDS             complement(33261..34769)
                     /locus_tag="PW109_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        11493 a         6388 c         7583 g         9400 t
ORIGIN      
        1 agtagaaaaa aaatatgcta ttatttgtat tgaggtgatc gtgaaaatgc ttacagagga
       61 acgtagacaa aaaatcctag acttgcttga acagcagaaa atcgtgaaat cacaagatct
      121 tgtaaacctt tttaatgctt ctgaatcaac gataagacgt gatttacaag agctggagaa
      181 tagtgggttt ctagaaagag ttcatggcgg agctaagaaa gagcaattat tgaattttga
      241 gcagaatatg agtgaaaaat cactcaaaaa cgttcacgaa aaacagcaaa tcgccaaatc
      301 ggcagctgaa accattaatg acgaagatgt catttattta gacgctggct ctactacgtt
      361 agaaatgatt ccttttctgc aaggaaaaca gatcacagta gtgacaaatt ccgttcaaca
      421 tgcggcaaat ttagttgatt tggagcttgc taccattatt ttaggtggaa cgataaagct
      481 ggcaactaat gctgttttag gatctaatgc gatagaacaa ctaaaaaatt ttcattttaa
      541 taaagcattt atgggaatga acggtgccca tcttgaacaa ggattcacga ctccggaccc
      601 agaagaggca gctgttaaac gtttagccat gaaaaattcc gaagagacat ttgtattgat
      661 tgatcatacc aaattcaacc agttgtcctt cacctctgtt gcacctttgg aagcagccac
      721 gatcataacc gagcgttgtc cattagaatt cgttaatgaa ttccgtgaaa agacaacaat
      781 taaggaggca atacaatgat ttacacagta acacttaatc catcaattga ttatatcgta
      841 cgcgtagatg ggttaaaact aggcgatttg aaccgaatga aggaagactt caagttacct
      901 ggaggaaaag ggatcaatgt ttcccgtatt ttgaaacgga tcgattctca atctacagca
      961 ctagggtttt taggagggtt cactggcgaa tttatttcga agtggcttga aaaagaacaa
     1021 atccagtctt tgttcacaac cgtaaaagaa gatacaagaa tcaatatcaa actaaaatca
     1081 gatactgaaa ctgaaattaa cggtttgggc ccatctattt cagatgaaga aatcgatgag
     1141 ttgaagcaag cattaggaac agtaggacaa ggagatatcg tcgtattgtc aggaagcgcg
     1201 ccggctagcc tgcgaaaagg attttatcaa gaactgattg atatcattcg agaaaaaggt
     1261 gcagatttcg tgattgatac aacaggtgat gacctaaaaa atgcacttgt taagaaacca
     1321 ttgttgatca aaccaaacaa tcacgaatta gctgagcttt acaatacaac cttccaatct
     1381 gtagaagaaa tcattccgta tggtaaaaaa ttgctagctg aaggggcaca gaatgtgctg
     1441 atctcaatgg ctggagacgg tgctttgctc ttcacggaac aaggaactta taaaacgaat
     1501 gtattagttc gcccattgaa aaattcagta ggtgcagggg attcaatgat tgccggcttt
     1561 attggggctt acagccgtac gaaagatcca atcgaagcct ttaaatgggg agttgcttgc
     1621 ggaagcgcaa cagctttttc tgatgacttg gcagctcgcg aattgattga tgagttgctg
     1681 ccagaagttg aaatcacaaa aattgattag tttcaaggag gataatcatg aatatcaagg
     1741 atttaatggt caaagaagcg atgatcatgg atttgcaagc gactgataaa aaaggcgcaa
     1801 tcgatgaaat ggtccaaaaa atgtttgatg ccggccggat ttccgatatc gaaacatata
     1861 aagaaggaat tctagcccgc gaagcacaaa cttctacagg tcttggtgat ggaatcgcca
     1921 tgcctcacgc gaaaaacagt gcagttaaag aagcaacagt tttattcgca aaaagccaaa
     1981 aaggagtaga ttatgaagcc ttagacggac agccaactta tctgtttttc atgatcgcag
     2041 ctccagaagg cgcaaacgac acacatttgc aagcattagc agcactatct cgattgctga
     2101 ttgatgcaga tttcgtagga aaattaaaag aagcaaatac accagaagaa gtccaagact
     2161 tgttccaaac agctgaagag caaaaagaag cagaagaaaa agcagaacaa gaaacacaag
     2221 ctgcagaagc ttctgctcat ccagaaagaa aattcgttgt tgccgttaca gcttgtccaa
     2281 cagggatcgc tcacacttac atggcagaag atgctctgaa gaaaaaagcg aaagaaatgg
     2341 gtgtggagat caaagtcgaa acgaacggtt cagaaggaat caaaaaccga ttgactgctg
     2401 aagacatcgc ccgtgcagaa ggtgtaatcg ttgcagcaga caaaaaagta gagatgaatc
     2461 gctttgacgg aaaagaactg atcaatcgtc ctgtcagtga tggtatccgt aaaccagaag
     2521 agctgatcaa tctagccctt agtggtactg caccagtatt ccatggtgat ggaaaagaaa
     2581 gcagttcaga agaaggaaat gcggatggta ctatcggaca aagaatttac aaagatttga
     2641 tgaacggcgt atcacacatg ttacctttcg ttattggtgg aggaatcgct attgcgcttt
     2701 ctttcatgat cgaccaattt attggcgtgc cacatgatca attatcaaat ctagggaatt
     2761 acaatcaagc tgctagctgg ttcaaccaaa tcggacaagc tgcatttggt ttcatgctgc
     2821 cggtattagc aggatttatt gcttcaagta tcggtgaccg ccctggtttg atcgttggat
     2881 ttgctgctgg tgcattagct aatacaggcg gtgcaggatt cttaggtgca ctgattggtg
     2941 gattcttagc tggttatgtc atcgtatttt tacgtaaatt attcaagaac ttaccaaaat
     3001 cattggatgg aattaaaacg atcctctttt atccagtgtt tggcttactg attactggtt
     3061 tcttgatgtt gctggtcaat gtccctatga aagcaatcaa tgatgcattg aacggtttct
     3121 tgacaagtat gagtggttcg aatgctgctt tacttggtgc attattagct ggaatgatgg
     3181 cggcagattt aggcggtcca atcaacaaag ccgcttatgt gtttggtaca gcaactttag
     3241 ctacgactgt tgcaactggc ggaagtgtcg tgatggcttc tgttatggct ggaggaatgg
     3301 tacctccatt ggcaattttc gttgctacac gtttgtttaa gaataaattc agcaaaacag
     3361 atcaagatgc tggtttgacg aacatcgtga tgggactatc gtttgtcacg gaaggatcga
     3421 ttccatttgc agcagctgac ccgattcgtg ccattccaag ttttattatt ggttctgctt
     3481 taactggcgg attagtcgga gctttcggga tcaaattact ggctcctcat ggcgggattt
     3541 tcgttgtatt cttactgagt cacccggtca tgtatttggt gtttattgct atcggagcag
     3601 tcgtatctgg tgtgatttat ggtttattga gaaaagctcc tgcagcagca gaagcataga
     3661 aataaaaaag aagaggctgg tttttcccag tctcttcttt ttttatagaa tcatatagaa
     3721 tcaagtagtg tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt
     3781 gcttcaaatt tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg
     3841 cttcatcagt caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca
     3901 agatttcctg atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt
     3961 ctcgtaccat taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc
     4021 ttgcaccttt tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt
     4081 aaattggatt accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta
     4141 aaggcatcag atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg
     4201 gtgcttttcc aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt
     4261 agattggatc catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc
     4321 ccaatgtcaa tatcggaagg aaaatccccc ctggaagacc accaccgtaa gaaatcatgg
     4381 aaaaaacgaa tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc
     4441 caaaagctaa aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg
     4501 gcaaaatcag caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt
     4561 aaaattttgg caaagccagc aagaccttct gataaataaa acctaagatg cctagaagta
     4621 ctcctaagat cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat
     4681 ataaaacagg tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag
     4741 caaatgatgt cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga
     4801 gtaatcctgc gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa
     4861 ttaaaatctt ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg
     4921 atgcgccaag ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa
     4981 gtacaccgcc aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc
     5041 gaagctgtcc ctctacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca
     5101 gataacccac gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg
     5161 gctgttcccg actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata
     5221 atcgaaacag gctaacgacg attcctgtca gcagtccaac aaatattcct tttagaataa
     5281 aatatatctg agtcttatct aatttcttga tttttccttt tttttccatt caaacgcctc
     5341 gctttctttt aatttagcgc gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa
     5401 caaatgattt tgaactagct agtttaatga aagccaagaa aatgctataa ttaacaagat
     5461 tgataaaaga aaggatgttg ttaatgtcat ccaaagctga attattaaac gggatgaacc
     5521 cccgtcagaa agaagctgtt cttcacactg acggaccatt actgctaatg gcaggtgcag
     5581 gaagtggaaa gacgagagtg ctgacacacc gcattgccta tttgattgag gaaaaagaag
     5641 tcaatccttg gaacatccta gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag
     5701 aacgtgtcaa tgcgattctc gcatctggag gcgaagatgt ctgggtatcg acttttcact
     5761 cgatgtgtgt gcgtatttta agaagagatg ttgattttat cgggtataac cggaacttta
     5821 caatcatcga ttcttctgaa cagctgacct tgatgaaacg gatactaaaa gaactgaaca
     5881 ttgatccgaa aaaatatgat cctcgaagta tattaggaac aatctctcaa gcaaaaaaca
     5941 gcctgcagac ccctcaggat tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg
     6001 caaaatgtta tgctgcttac caaaaagagc ttcaatataa tcaatgtatg gactttgacg
     6061 acctgatcat gaatacgatt cgattatttg aagaacatcc tgattcactc acatattatc
     6121 aaaacaaatt ccattatatt catgtagatg agtaccaaga tacaaatcac gcacagtata
     6181 cgctagtcaa tttattggca ggtagattta ggaacctgtg cgtcgtggga gatgcagatc
     6241 aaagtattta cggctggcgc ggtgcagata tgcagaacat cttggatttt gaaaaagatt
     6301 atccagatgc tgcagtaatt ttgctagaac aaaactatcg ctccacgaaa aacatcctta
     6361 gcgcagcaaa ccaagtcatt gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag
     6421 aaaacaaaga aggaaacaag atcacttact accgtgcaga taatgaacga gacgaaaccc
     6481 gttttatcgt agatcggatg caagaagaga ttcgaagcaa tcatcggaat tacggcgact
     6541 ttgccatctt gtaccggacg aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag
     6601 caaatattcc ttataaaatg gttggagggc acaagttcta cgatcgtaaa gaaatcaaag
     6661 atattttagc gtatttgaat gtgctggcaa atcctcaaga ttcaatcagt tttgagcgta
     6721 tcgttaattc gcccaaacga ggcatcggac caggatctat cgaaaaacta cgcagttttg
     6781 cttctcttca tgaatggcca ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg
     6841 gtggaaaagc aggacagcaa ttaggtgcat ttggagagat gatccaagaa gtcactcaga
     6901 tgatcccata cttgacagtg acagaattga cgaaagaggt tttggatcgt agcggatatc
     6961 tagaagactt aaaaatccaa aatacattag aagcacaagc acgcatcgaa aacttggagg
     7021 aatttttaac ggtcacacaa gaattcgaca aacaattcga acagcaaaat gaagaagacg
     7081 ctgatgcacc agaagaaaaa ctaacggtct tcttaaatga tctggcactt gtttccgata
     7141 ttgacaattt agaagaagat gcttcccaag tgacattgat gactttgcat gcagccaaag
     7201 gattagaatt cccagttgta ttcctgattg gtctagaaga gggggttttt cctttatctc
     7261 gagcattgat ggaagaaagc gaactggaag aagaacgccg tctcgcatat gtggggatca
     7321 cgcgagcaga agaagcgttg tatttaacaa atgctttctc cagaacatta tatggacgga
     7381 cgcaatataa ccgaccttct cgttttgtag aagaaatcga tcaggagtta ctggaaatcg
     7441 aaggcatgcg tcctacgcct aaaaaaacac ctgtattcgc taaaaaaaca gcatatagct
     7501 ataaacagcc agagacagcg gttgttccct ctaaaagtgc tactggaggg gaaaataaca
     7561 gctggaaacc tggagataaa gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag
     7621 taagcgggac gtcgaaggat ctagaactag atgtggcttt cccaagtcaa ggagtaaaac
     7681 gtcttttggc tgcctttgcg ccaattgaaa aagcttagta gtggagggta aatgatggaa
     7741 catgaaatga cgattgacga agctgctaaa cgagcagaag aacttcgtac aagactgaac
     7801 caatggtctc gcgaatacta cgtagaagat aaaccaacag tggaagacta tgtttatgat
     7861 aaagaatacg cagaattagt agcaattgag gaacaatatc ctgatttgat cacgtctgat
     7921 tctcccactc aacgagtagg cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat
     7981 attccgcttt acagtttgaa cgacgttttc agcaaagaag agctgatagc ttttgatcaa
     8041 cgtgtacaaa aagcagtggg acgtgtagtt gactattgct gtgaactaaa aatagatgga
     8101 ctttccgttt ctttgcgtta tgaagatgga aatttcgtga gaggtgcgac tcgcggggat
     8161 ggaactgtag gagaaaacat cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa
     8221 ttaaaagaac caatgaacat cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt
     8281 gtccaattga accaagacag agaagcagag ggaaaagata tctttgcgaa tcctcggaac
     8341 gccgcagctg gttctttgcg tcagttagat tctaaaatca cagcgaaaag aaatctggac
     8401 acttttttat acacagtagc tgatttcggt cctatgcaag caaaaaccca gtatgatgcg
     8461 ctagaagagc tagaaaaaat tggattccac acgaatcggg aaaaacgtct atgtcattcg
     8521 attgatgaag tgtggtcata cattgaagaa tatcatgaca aacgtgtgga tttgccctat
     8581 gaaatcgatg gaatcgtcat caaagtgaat gaattctcgc tacaagatca acttggtttt
     8641 actgtcaaag caccgagatg ggcagcagct tacaagtttc cgccagaaga agttgaaacg
     8701 ctaatcgaaa acatcgaatg gacagtcggt cgaacagggg tagtaacgcc aacagctatc
     8761 atgacaccag tccgagtagc aggaacgaca gtaagccgtg ccagcctgca taacggtgat
     8821 tatattaagt tgaaggatat ccgcttgaaa gatacagttc tgatttataa agcgggagac
     8881 atcatcccag aagtttcgca agtcgtgtta gataaacgac ctaaagacag cgaagaatac
     8941 caattgccta cacattgtcc agtttgtgga agtgaacttg tccatttgga tgaagaagta
     9001 gccttacggt gcatcaatcc taaatgccct gctcaaatga aagaaggttt gaaccacttt
     9061 gtttcaagaa atgcgatgaa tatcgatggg ttggggccgc gcgtactaga acagatgtat
     9121 gataaaaaac tagtagcaga tgttgctgat ctttacaaat tgacagaaga agagttattg
     9181 acgttggaca aaataaaaga aaaatcagca aataatattt tgacagcaat cgacaacagc
     9241 aaagataatt cggtcgagcg gttgattttc gggttaggaa tccgacatgt cggagcgaaa
     9301 gcagcaaaaa ttttagcaga acatttcggt gatctagaaa cattgagtaa aagtgactat
     9361 gagtcgatta ttgcgcttga tacgatcggt gatatcattg ccgacagtgt agtcacttat
     9421 ttcagtaacg aagaagtcca tgaattgatg aatgaattaa aacaagcagg cgtcaatttc
     9481 gaatacaaag ggcttcgaaa tgcccaattg caagaagtcg agtctccttt taaagaaaaa
     9541 acagtagtcc ttactggtaa actaacacga tttactcgtg aagaagcaaa agagacgatc
     9601 gaaaatcttg gcgggaaagt gactggtagc gtatcgaaaa aaacggatat cgtggtagca
     9661 ggagaagatg ctggcagcaa gctgaccaaa gcacaagaat taggaatcga agtttggaca
     9721 gaagatcaaa tggcagatgc attggctaaa agtcgctcag tagaagaatg aaaaatgagt
     9781 tttttcagac aaatttcaat aaacaatttc agacacggca ctcttgtgtt aaaatagaga
     9841 gcaaagtttt taactcgttt agaaagaagg ggtagtatgg cgattagtga agaacaagtg
     9901 aaacacgttg ccaaattagc aaaacttgcg ttcgcagatg aagaacttgc aagttttacc
     9961 gaccaattag gtaaaatcat cgatatggtt gaaatgctgg aagaagtaga tacagaaggt
    10021 gttccattta cgtcgaatgt ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa
    10081 gctggatgga accgcgatga attattgaaa aatgtgccgg aaacagaaga cggctttatc
    10141 aaagtacccg caatcatcga taatggggag gctggcgcat aatgacagaa ttacacaatc
    10201 gttctctaga agaactgcat catatgcttg tatcgaaaga aatcactgta caagatttga
    10261 caaaagcaac atttgaacga atcaaagaaa cagagcctga ggtcgaagca ttcattacat
    10321 tgaacgaaga aaaagcattg gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa
    10381 gcaacgtcct tgctggtatt ccaattggta tcaaggataa tatcgtaaca aaagatatct
    10441 tgacaaccgc tgcatctaag atcctttaca attttgaacc aatttatgat gcaacggtca
    10501 tggataaaat ctatcaagca gatatgatcc ctgttggtaa gctaaacatg gatgaatttg
    10561 ctatgggtgg aagtacagaa acttcttatt tcaagaaaac aaaaaatgct tgggacccaa
    10621 caaaagtacc tggtggctct tctggtggtt ctgcagcagc agttgcagct ggacaaattc
    10681 cagtttcttt aggtagtgat actgggggaa gtatccgcca accagcagct tttaacggca
    10741 tcgtagggat gaaacctact tatggccgtg tctcacgttt tggtttgatt gcctttgctt
    10801 catctcttga ccaaatcgga ccaatgaccc gtacggtgaa agataatgca ttagcattga
    10861 cagctatcag cggttatgac gaaaaagacg gtacttcttc aggcgtttca gttcctgatt
    10921 ttgcagaggg actgacaggc gacatcaaag gaatgaagat tgctttacct aaagaatact
    10981 taggcgaagg tgtggcccct ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc
    11041 gttcattagg tgctgttgtg gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg
    11101 tttattatat cattgcctca tcagaagcaa gttccaactt gcaacgattc gatggtatcc
    11161 gctacggtta tcgctcagaa aatgtgaaat cattagatga tgtatatgtc aactcacgtt
    11221 cagaaggatt cggtgaagaa gtaaaacgcc gtatcatgtt aggtacattc tccttaagtg
    11281 ctggttatta tgacgcttac ttcaaaaaag cgggacaagt ccgtacattg atcaaacgag
    11341 attttgaaaa agtctttgca gattatgact tgatcatcgg gccatcttca ccaacagttg
    11401 cattcggaat cggtgagaac attaacgatc caatcacaat gtacatgagt gacatcttaa
    11461 cgatccctgt caacttggct ggacttccag gtatgtctgt accggcaggt ttatcagaag
    11521 gattaccagt tggattgcag atcatcggta attattttga tgaaaaaacg atgtatcaag
    11581 cggcatatgc gtttgaacaa gcaacagaat tccataaaca acaaccagct attttaggag
    11641 ggaaagacga atgaattttg aaacagtcat tggactagaa gtccacgtcg agttaaaaac
    11701 caactcaaaa attttctctc cagcgccagc tcattttggt gcagaaccaa acagcaatac
    11761 aaacgtgatc gactggggtt atccaggcgt gctgccagta atgaataaag ctgcgctgga
    11821 attcgggatg aaagcagctt tggcattaaa ctgtgaaatc tctaaagata ctcattttga
    11881 ccggaaaaat tacttttacc cagataatcc aaaagcatat caaatttccc aatttgatca
    11941 accaatcggt catgacggat ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg
    12001 gatcgaacgt gtccatttag aagaagatgc tggaaaaaac atgcatggta ttggcggtta
    12061 ttcttacgtt gatttgaacc gacaaggcac gcctttgatc gaaatcgttt ctgaagctga
    12121 tatgcgttca ccagaagaag catacgctta tctagaagca ttgcgatcaa ttatccaatt
    12181 tacagaagtc agcgatgtga aaatggaaga aggctctatg cgctgtgatg ccaatatttc
    12241 tcttcgccct tatggacaag aagagttcgg tacgaaagca gaattgaaaa acttgaactc
    12301 tttatctttc gtgaaaaaag gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct
    12361 ttcaggagga gaaatccaac aagaaacacg ccgttttgac gaaacaacga ataaaacagt
    12421 tttgatgcgt gtcaaagaag ggtctagtga ttatcgttac ttcccagagc ctgatatccc
    12481 gaaattcgtg atcgatgacg aatggattca aaaagtaaaa gctagtttgc ctgagatgcc
    12541 agcttctcgt cgcgaacgct atatccgtga gtttggtctt ccagagtatg acgcaatggt
    12601 attgacactt acaaaagaaa tgtctgattt ctttgaagct gttttggaaa atggtgcaga
    12661 tgccaagcaa gcatctaact ggttgatggg tgaagtttct gcttacttga acagtgagaa
    12721 actggaatta gctgagacaa aattgacacc tgagaactta gcaggaatga ttcgtttgat
    12781 cgccgatgga acgatcagct caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa
    12841 tggcggagaa gcccaagaag tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa
    12901 caaactactg ccaatcatta atgaaatttt ggataacaac caacaatctg tcgatgattt
    12961 caaaaatgga aaagatcgtg cggttggctt cctagtcggt caaatcatga aagcaactaa
    13021 aggacaagca aatcctggtg tcgtgaataa gctacttaaa gatgaattag cgaaacgata
    13081 gaggctgaaa atgaaaaaag cacgcgtaat ttataacccc acctcgggaa aagaattatt
    13141 gaaaaaaaat ttggcggata tcctacaggc attagaagaa gcaggatttg aagccagcgc
    13201 ctatgcaact acaccagaac cagattcagc caagaatgaa gcacgtagag tcgcagaact
    13261 aggttttgat ctagtagtgg cagctggtgg cgatggcaca atcaacgaag tggttaatgg
    13321 aatcgctccg cttgaacacc gtcctaaaat ggccatcatc ccagctggta caacgaacga
    13381 ctatgctcga gcattaaaaa ttcctagaga taatattaaa gcagcagcag aagtgattaa
    13441 aaagcatcaa accgtaaaaa tggacatcgg cctttctgat aacagttact tcatcaatat
    13501 cgctgcaggt ggctatttga cagaattgac ttatgaagtt ccatcagaac taaagagtat
    13561 ttttggttat cttgcttatc ttgcaaaagg agcagagatg ctccctcgag taaagcccat
    13621 caaaatgcgc ttgaagtatg atgaagggga atatatcgga aatgcttcga tgtttttcct
    13681 agggctgacg aactctgttg gaggattcga acagatcgcg ccagatgcca aattggatga
    13741 tgggaagttt tctttaatca ttgtgaagac agcaaatatc tttgagatct tgcatattgc
    13801 ggcactcatg ctgaatggcg gtcgacatgt cgatgatcca agagtaatct ataccaaaac
    13861 aagcagtttg ttcgtcgaaa cagaagatga tatcaatcga ccagtaatga tcaacttaga
    13921 tggtgaatat ggtggggaag caccgatgca tttccaaaat cttcatcagc atattgagtt
    13981 ctttgccaat accgatcaga ttcctgatga agcaatcaca ggcacagatg aagaagaatt
    14041 agaggttgtt agcaaagaat ttgtaaaaga agtcgaacgt ttaacggatg aagatatcga
    14101 cggtgacgga aaaatagcag acgaaacacc taatcaaaag aattaatcaa aagccggtgc
    14161 tgaacgtagt aaggtcagtg ccggttttag ttgtgtaatt agctgattta tagtagagtt
    14221 cagtaggatt gaaaaatagg cttaaaggag caaaatatga cagaacagca aatagtaaag
    14281 aaaaacgaac gatatacagt cgaaatcgtc gacttgagtt atgaaggaat gggggtagca
    14341 aaaatcgatg gttatccatt atttatcgaa aatgcactcc ctggagaaga agtagaaata
    14401 ctagtagtaa aagcaggcaa taagtttgga tatggaaaag tcgaaacatt tattacacaa
    14461 tcacctgatc gtcagcctgt agaaaacaat gcattacttc gcacaggtat cgcaccttta
    14521 gcccatttga agtatgaaca acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg
    14581 gcgaagattg ctaagatgcc agaagttcct gtacttccaa cggtcggaat ggcagaacca
    14641 tttggctacc gtaataaagc acagatccct gttcgccgta tcgatggaca gttagccacc
    14701 ggtttttaca aaaagaatag ccatgatcta gtccctatcg aagattttta tatccaagat
    14761 cctgtcatcg atcaagcaat caaagtagtc agagatatct tgcaacggtt ccaagtacga
    14821 ggatataatg aagaaaaaaa tgaaggacag cttcgtcaca tcattatccg tcgtggtcat
    14881 tattcccatg aaatgatggt cgttttagtt actcgtaaag aaaaattctt taaatccaag
    14941 gaaattgctg aagccattca tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc
    15001 aatgaagaaa aaacaaatgt gattttagga gacaaagaaa aagttttata cggcagagac
    15061 tatatcgaag atcagctatt aggaaaaacc tatcgaatct cagcgaagtc attttatcaa
    15121 gtaaatactc aacaagcaga gtatttgtat caaacagcca tcgaatttgc cgagttggat
    15181 aaaaaagaca ttgtgatcga tgcttattca ggaataggta caatcggatt gtctctagct
    15241 gacagagtag ataaggtata tgggatggaa gttattccag aagcaattga agatgcacag
    15301 ttcaatgcct taaataataa aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa
    15361 gtcatgaaaa aatggcaaga aaaaggggtc aaaccatctg tgatcgtagt agaccctcca
    15421 cgtaaaggat tagatcatcg ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc
    15481 gtctatatct cttgtaatcc agcaactttt gcaagagatg ctaagttatt tgcagaagca
    15541 ggttacgccg tgaagaaagt tcagcctgta gatcttttcc cgcaaaccca tcatatcgaa
    15601 ttagtcgctt cattcgttaa aggagaataa aaatggacca aaaagaaaaa atgaaagaat
    15661 tgatcaaaaa gaaacaaggt ggaggccgat ccaaacaaat ggaaacacct aaaaatgatc
    15721 gtaaaaacat gagaaaaggc cctaaaatct ttaataaata gacgtgctaa agaaagacta
    15781 agaagcgtta acttttgtta ttgtttctta gtctttttag ttagtctaca ttattttggc
    15841 gatttgccaa taaggactgt tgagttgtgc aaaatcaaat ctgggtcaga atgaacgtca
    15901 ttctgtctca gatttttcgt tatttcaatt tgattaactg attgatcaaa aagattttta
    15961 ttttcatatt ctcaaatgat tcaacactaa atatacatat ttgacgcgga aattattatt
    16021 cgatttgggc ggcatcaggg ggttatgacg acgtagcaga cccattattt tactcaagaa
    16081 aatgtccgtg cctactattt agaatacgat agtgaacgtg ctgggagctt tgttctttta
    16141 gagaaagtct ctgaagataa attagttgtc ttagattttg actgtaacga aaaatggcga
    16201 aatggaagag caccagcaag tgattgaccg gatctacgaa gcgacacgat atctgccttt
    16261 ggatcgtttg tgattaagtc ctcaatgtgg atttgcttca acagaagaag aaaatgtttt
    16321 gtttgaagaa caatggaaaa aatttctttt gtcaaaagca ttgcagaaga agtttgggga
    16381 aaatcattaa atcatctagg agagcttgat aggaaataac actatggtat aatcagaatg
    16441 cttaatacta actactgagg gagccttatg aaaaagaaaa ttcttaaatt tatcggatgg
    16501 ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc ttgccttcca gctttccccc
    16561 agacctggag cattcttgat caaccatatg ttttcaaata ctgttcaaat cactgataaa
    16621 aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa cagatttgac gtatgagtcc
    16681 aagtataaag agaatactta tgacatctac tatccaaaaa acagcaaggg gcctgttcct
    16741 gttctatttt gggtacatgg tggaggattc gtcggcggag ataaatcggg tgtcaaagaa
    16801 tttgcgacaa aattagtttc agacgccaac atcgcagtgg ttgcgatgaa ttatgaatta
    16861 gcgcctgatt cagaataccc taatcaagtt cttcaagtga atgaactgat taaagattta
    16921 ttagctgaag aaaaagacga caaattgcta gacatggaac aattattttt tggtggagat
    16981 agtgcaggtt cacaaatcgc attgcaatat gcagctatac aaacgaatga cgcgtatgct
    17041 aaacaaatga aaatcaaaca gctacttcca aaagatagtc tgaaaggaac catttcttat
    17101 tgcggacctg ttgatttgaa gctaacagca cagcagcatt cggatgatcg atttatgaag
    17161 tttttcgtca aaaccgttgc gtggtcgttg attggaacaa aagattggaa aacgagtgag
    17221 caattgaaag aagctagtgt ggctgaccat gtttctaagg aatttccgcc aacgtatata
    17281 actgacggaa atgcatactc attccaggag caaggtcaag cattagaaaa tcggttgaca
    17341 gaattaaagg tacccgtgca atccttgttt tatacagata caaaaaaaga gattacccat
    17401 gaataccaat ttgattacac gctaaaagaa tcgcaaaatt gttaccagca gacattggat
    17461 tttgtgaata agtataagta agaggttgtg aaaaagccgt ttcgttttga gtatgaagaa
    17521 acgaagcggc ttttcacaac ctctctttca ataccttttt gttcttggga taaaactttc
    17581 cctaacaatc aatcgtgcag gataagtcaa aatgattggt tcgtcccgta taccgtcaat
    17641 tcgctccaat gccattcgaa cagctgcttg cccaattgat ttcgcattga tttgaacact
    17701 ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta tcaaagcttg ctacaacaat
    17761 ctcttctcca atttggatat ttttttgttt caatgcccga tagacaccaa ttgccattgg
    17821 atcgctcccg acaagaatgg cagtaggaag aggggattca ttttctaata gattagtcgc
    17881 aagtacctca ccttgttttt ctgtccagcc acctagatga taagacatat atttttctaa
    17941 gtgatgatcg atcataaaat gctgataggc acgcatcctc ttttcattta tagaaatctg
    18001 cttcccaccg aattcatcca tatcgacttt atatcccccg atataggcta ttcttttatg
    18061 accattttct aagaacaatt ccagtacggt tttagtcatc cgttcaagat ctgcatacac
    18121 catatcgacc tcttgctgga tgtcagggtt atccacgacg actatttgca tgttttgttt
    18181 taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca acgatcaccg cacctaactg
    18241 atctagatct tcccattgtt taggattatc agatagatta tagattcgat tcatccctaa
    18301 atgcagacgt cgagcttctt cttccatccc tactcgcaaa tgtttgtaat agatatcctg
    18361 ttcttcgctc gctttgccga tcgtagagat cacaccaatc gaaggcattc tctttttgat
    18421 atatttacga gtactgatgt cataatccaa cgcagcagct gcctctacta tccgttgccg
    18481 agtagtctga gccacatgga tcgtctgatc gttattcaac acgcgggaaa ctgtagcagg
    18541 agagacattt gcctttcgtg cgacatctct aattccaacc atttttacac actcctcaaa
    18601 aaaataaaaa atttttattg acaaaattgt ttagtaaaca gtatacagga attataaaaa
    18661 aagtaaacgg tttccgtttt gttgggagtg aaatgatgga tattacgcat agaaaaacag
    18721 ataaaaattt catcttattt gatgaaaaga gccgagtctt ccatttacga aatacatttc
    18781 tttcttatct gataaagata gaagaaagca atgtattggc gcatatttat tttggcaaac
    18841 cagtcaagca gtacaaagac aataaaaatt atccccgaag agatcgtgga ttttcaggaa
    18901 atgttccctt aaatcctgac cggtctctgt cgaaagatac actaccgcaa gaatactctt
    18961 ctcatggcag catggacttt cgaacgcccg catcgatcat tcagcgcaag aatggatcag
    19021 acttactgga tctgagatat gacagccatt atattacaga tgggaagcca gagatcgaag
    19081 gcttgcctca aacttatgtg atggacaagt cagaggcaca aacgttggtg atttcactta
    19141 aggatagaga aacagcgatt tatttcgatt tgttctatac catatttact gatagagcag
    19201 tgatcacacg atctgtaaag ataagaaatg aaaccggaga aacaatcaaa ctagaaaaag
    19261 cggctagttt ccagctagac tttgcccata ctaggcgatt tgatgaagtc attgctttgc
    19321 caggagcaca tgtcaatgaa agacagattt cacggcaatc tgttttatct ggaacaaaag
    19381 tatttgaaag ccgtcgagga acgagcagtc accatatgaa taactttatt gctttaattc
    19441 atcatcatac gacagaaaat actggagaag cgatcggact tcaatttgtt tattccggca
    19501 accattcttt cgaacttgaa aaagatcaga tcaatcaatt gagagtcgtc ggtgggatca
    19561 acagccatcg tttttcgtgg gaattgaatg caggccagtc ttttcaaaca cctgaaatga
    19621 tcctttctta ctcaagtcag ggattgaaca aaatgagtca gatccaccat gagttgctac
    19681 gtgaaagaat cgcaagaggt cgtcatcaat ttgctgaacg gccgattctg gtcaataact
    19741 gggaagccac ttactttgac ttcaatagtg aaaaaatcaa ggcgatcatt gatgaagcaa
    19801 aggagctagg catcgaaatg ttcgtattag atgatggctg gtttggaaaa agagatgcgg
    19861 acaattcttc attaggagac tggttcgagt atgaaggcaa gttgacaaat ggattacgtg
    19921 aaatagcaga ttatgctcat agcaaagggt tgaaatttgg tttatggttt gaacctgaaa
    19981 tgatctcggt tgattctgaa ctgtatcgaa cacattctga ttttcttatg caggagccag
    20041 gtagaatgcc ttctgcttcc agatcacaac atgtactgga ttttacaagg ttagatgttc
    20101 ggcagacgat tgagaaacaa atgagaaaga ttttagatac gattcctctt gactacatca
    20161 aatgggatat gaaccgttcg ctttcagatg tctattcgat tacccttgac ccacagagac
    20221 aaggagaagt agcgcatcgt tatatgctcg ggctttatga actacttgaa catttgataa
    20281 ctgattatcc ggaaatctta tgggaaggct gttctggtgg aggtggacgg tttgatgccg
    20341 ggtttattta ttatatgccg cagtcatgga caagtgataa cacggatgca gtggagcgta
    20401 tgaaaatcca atatggaaca agtcttgctt atccgatttc gtccatcaca gctcatgtat
    20461 cagctgtacc aaatcatcag accgacagat ctacttcgtt gaaaacaaga ggagaaacag
    20521 ctatgagcgc tgtttttggt tatgaactgg atttgacaaa gctgtctcct gaagaaaaaa
    20581 agcaagtcaa agaccaaatt atatcctatc aaacgattcg tccggtgatc cagtatggcc
    20641 attattatcg tttggcgagt ccttttgaag aaaatattgc cgcatggatg tttgtgtctc
    20701 ctaaacaaga tgaagctatt gtgtttttag ggaggatact agcttcagct caaccagcgt
    20761 tccatgaagt atatctgatg gggttagatg atgaggcact ttatcaggaa cagacctcga
    20821 agcggatatt ttcgggggcc gaattgatga cagtcggact ttacttccca gattttcaag
    20881 gtgatttcca aacagagctg cttcatttca aaaagttatg agagagaagg aaaaaagtat
    20941 gaaagcaaat ataaaatggt tagatgatcc acaagttttt agagtgaacc aatcaaatgc
    21001 acatagcgat catgtttgct atgcaacaag ggaagaagta gaaaccacat cctcccttaa
    21061 acagtcttta aatggaacat ggcgtttttg ttattcgcca aatccttcgg tacgtccaaa
    21121 aacattttat aaaaaagatt ttgatgcctg ttcttttgat gaaatcactg ttcctagtca
    21181 tattgaatta gcaaattatg acaagatcca ttacatcaat acgatgtatc cgtgggaggg
    21241 acatgtattt cgccgtcccc ctttcacgac aagccctgag aaagaggcgg aagggatatt
    21301 ttcaactgct aaagacaatc cggtgggttc gtatttgacg acttttcgat tagaagaagg
    21361 tctaaaggga aaacaaatct cgattcattt tgctggtgta gaacaagcaa tgtttatttg
    21421 gttgaatggt tattttatcg ggtatgccga agattcgttc actccgtctg aatttgattt
    21481 aacaccttat ttggaagaag aaaatattct agctgtggag gtttacaaaa gaagtacagc
    21541 tgcattcttg gaagatcagg actttttccg attttttggg atcttcagag atgtctactt
    21601 agttgctcga ggacaaaacc atttagaaga tctttggatc aaacctcgat tagatgacaa
    21661 atgtcaaaat ggcaaacttg aagtagattt gtcttttgag caagtagcca aaaacagtca
    21721 gctgaatgta gcgatactag atcctgaggg gaaggaaatc tatcaacagc aatatccgtt
    21781 aaaagagcaa atgacgattc ggatcgatgg atttgaaaac gtggcactgt gggggcattt
    21841 tcagcccaat ttatataagc ttgaattgga gacgatagac gaagaaaaaa gagtaacgga
    21901 gtatattgac tattccttcg gttttcgtaa aatcgaaatc aaagataaaa tcatctactt
    21961 caatggaaaa cggatgatca tcaacggggt aaatcgacat gagtggcatc caaaaaaagg
    22021 acgcgccatc actatagaag atatggaaaa agatttggct atcataaaaa atgcaaagat
    22081 caacggcgta cggacatctc attaccccaa ccagatcact tggtattatt tatgcgacca
    22141 agcaggcatc catgtgatgg cggaaacgaa cctcgaatcc cacggctcat ggcaaaaaat
    22201 gggcgcattt gagccttctt acaacgtgcc cggtgcagta cctcagtggc aggctgctgt
    22261 tcttgatcgg gcaaaaacga atttcgaaac attaaaaaac catccaagca ttcttttttg
    22321 gtctcttgga aatgaatcct atgcggaaga aaacattcga caaatgaacg attacttcaa
    22381 aaaagtagac cccgatcgac tcgtccatta tgaagggaat tttccaaatc cagcattcga
    22441 agattcgatt tcagatgtaa aaagctgcat gtatgcgcca ccagaagaga tcattgacta
    22501 ccttgaaaat aaagcggaaa aaccgttcat cttatgtgaa tacatgcatg acatggggaa
    22561 ttcgttaggt ggaatggatt cttacatgac gttgcttgat cgttatgaaa tgtaccaagg
    22621 aggctatata tgggatttta aagatcaggc gatccaagtg actgatccaa tcactggtag
    22681 agaagtgatg cgctatggcg gtgactttga cgataaacca agtgactatg aattctcagg
    22741 gaatggaatc gtttttgcag atggacaaga aaaacccgcc atgcaggagg taagatatta
    22801 ttatgaaaaa tacagtaaat aaaagtcata tggatacgga aaaagttgca atcgtcttcg
    22861 gcgactgtac attaggtgtc aaatcgggga atacgcatta tattttttct tatacaagag
    22921 gcggactgga atcgctcaat aaaaacggaa aagaatggct ataccgggaa acacttccag
    22981 ttttttggcg agcaacgacc gataatgaca gaggtagcgg atttggattt aaatccggta
    23041 tgtggttagg agccggattg tatccaaaag tcaaagaaat agaagtactg gtggacaata
    23101 aaaagatcga tttgccgatt gctccagtaa ataatcaata ttcaagtaat gaatacgctg
    23161 aaacggttga aatcattttt acactggaaa tcaacacagt accaaaaacc caagtgacaa
    23221 tttcctacca aatcgctgga gagggtaaaa tgcttgtcaa tatgcattat tatggacaaa
    23281 aaggattacc ggaattaccc gcattgggtt taaggttcat tatgccaacg aaagcaaaat
    23341 tttttgagta tgaaggccta tcaggggaaa catatccgga tcggatggct ggtggtgtcg
    23401 aaggtattta taaagtggca ggactccccg tttccccata ccttgttccc caagaatgcg
    23461 gcatgcatat ggaaacgaaa tgggtagaac taacaagaaa tcaaacgttg gataatacgg
    23521 ataaagatca agaagatttt tcgttgcgat ttgaacagca ggatacgcca tttgcttttt
    23581 ccgctttacc attcaaagca gaagaactgg aacaggcaac acatatagaa gaactaccgc
    23641 cagaaagacg aacggttctt acgatttttg caaaagtaag aggagtcggg ggtatcaata
    23701 gctggggagc ggatgttgag tcaccttacc gaatcagcag cgaagaggac catgaaatcg
    23761 gctttattat tcagtagcaa tctttagatt attatttgaa aaacggaatt tacaagacaa
    23821 agctgcttta ttctatatgt catgaacaag agagtaaaaa agttctcaga tacttttgtt
    23881 catgactatt ttcttcaata aaaccaattg ttatttttct gaaaaacaaa gttaactatc
    23941 agaaaattaa aaaaagtttc ataaaaatga ttgacagtcg atttttttat gtctatacta
    24001 ggtacaattc ttacatcagt ttggagcgat tttttatgaa atctagaaaa cttacaaaac
    24061 tgaatacgta ttactttagc tattttagat aggtttgtat tcatgacttc gtgggtacaa
    24121 acaaaccagg tttgtatcta tgatggctaa agatttttaa acacgactta cagccacgta
    24181 gagaaattca tctatagtgg ctattttata tttttgcagc gctttttaag gctcgttgaa
    24241 gatgttcact gtagaggcaa tttcctacag ggacatcttt ttttgttgat tatctgggtc
    24301 ataagtagga aagaaggagc gaaaatgaga acaacaggac acggtcattt agtaggaatt
    24361 aatgtaaaac caccttgaaa aagtagagaa aatcagccaa gatagataag taaacaaaag
    24421 aaagcgggaa aaacaatgat agtaattatg aaaaaagaag caacggaaga acaaataaaa
    24481 aaagtcgtcg atcatatcga aaaagaagga ttcaaagtct caatcgatca aggaacagaa
    24541 cgagtagtga ttggactaaa aggtgatacg cgaagtttac aagaaggagc atttacacgc
    24601 tatgaaggag tagaaaatgc tgtgcgtatt ttaaacacat acaaattgac ttctcgagaa
    24661 ttccatcctg gcaatacagt cgtggatgta gacggagtaa aaattggtga cggcagtttc
    24721 gtgacgatgg ctggtccttg ttctatcgaa ggattagacc aaatcagaga atgtgcgcga
    24781 atggccaaag ccgggggcgc aaaaattctg cgaggtggtg ccttcaaacc acgtacttct
    24841 ccatatgctt tccaaggttt agaagaagaa ggattgaaat acatccgtca agcggctgat
    24901 gaatttggaa tgaaagtgat tactgaagtg atggatgaag ggcatatcga tatggtggct
    24961 gagtacagcg atatcctaca gattggcgca cgtaatatgc aaaattttaa actgctatct
    25021 gctgtcggta aaacaggtaa acctgttgga ttgaaacgtg gaatttccgg tacgatcaat
    25081 gaatggctta atgctgcaga atacattgca gtcgaagaca aatctcctgt catttttatc
    25141 gaacgtggga ttcggactta cgaaacagcg acacgtaata cgtttgattt aagtgctgtc
    25201 ccattgatga aaaaattaac tcatttccct gtgattgttg atccaagtca tggtacagga
    25261 atttgggaac ttgtaccacc aatggctcgt gcgggtgtgg caagcggagc agatggcatg
    25321 atcgtagaga tccaccctga tcctgcaaat gcttggtcag acggtaccca atcgttgaat
    25381 gaaaagactt actcacgcat gatgaaagaa gttgcgatca tgaaagaagc aatggaaaag
    25441 attcagagac tcgattaatt ccagcttttt aaaagaaaga agtgaaacga atgcttactg
    25501 tacatgttga taaccaaaaa tacgatattc atattgaaaa aggcgcacta gaacaggctg
    25561 gatcatggct tcaaacgatt tggagacctc aaaaagtagc tgtgatcact gatacaaacg
    25621 ttgctccact atatgcagaa aaaatcatgt tccaattgag acaagctaat tttgaaccag
    25681 tcctttcaat cgtaccggcg ggtgagacca gtaaatcttt agaacaagca gctgagttat
    25741 acgatttttt agcagataat gattttaccc gcagcgatgg gatacttgca cttggtggag
    25801 gaatggtggg tgatctagcc gcattcgtgg cctcgacgtt catgcgtggg atccatttcg
    25861 ttcaaattcc gactacgcta cttgcacaag tagattctag tatcggcggg aagacagcag
    25921 tcaatacaaa caaagcgaaa aaccttgtcg gaacatttag ccagccagct ggtgtattga
    25981 ttgatcctga ggttctaaaa actttgccgg tacgtcgttt acgagaagga atcgctgaaa
    26041 ttatcaaatc agcagctata gcagatgcaa cgctttggaa acagctgaac gagctgaaag
    26101 acgaaaaaga tcttctggaa catgctgttc caatcattac tgctacgttg gaaatcaaac
    26161 gaaaagtagt ggaagaagat gtctttgacc aaggaaatcg acttatttta aattttggtc
    26221 atacaattgg ccatgcaatt gaaaatacag caggatatgg ggtagtcagt catggagaag
    26281 gagtagctat tggaatggta gccatcactt ctcatgcaga aaaaatcggc ttgtctccaa
    26341 ctggatcgac agctgcttta gaaaggatac ttcaaaaatt ccatctgccg attacgtacg
    26401 atctaagtca tccagatgaa ttattccacg cgataaccca tgataagaaa gcaagaggaa
    26461 gccaattgaa tatcatcttg ctagaaaaga ttgggcaagc aaaaatcgtt cctgttccca
    26521 cagagacatt caaagattac ttaggaggac tgtaagatgc gttatatgac tgcgggagaa
    26581 tcccatggac cagaattaac agcgattatt gaaggactac cagcaggatt acctctgtca
    26641 gaagaagaaa tcaatcacga actcgctcgc agacaagctg gttacggcag aggaggaagg
    26701 atgaagatcg aaaaagatcg tgttcggatc acgtctggca tccgtcacgg caaaacaatt
    26761 ggttcacctg tcactttagt ggtagaaaat aaagattgga aaaattggac aactgtcatg
    26821 tctgtagcag aagtgcctga aaaagaccaa tcactgcggc gagttgcacg tccgcgtcca
    26881 ggacacgcgg atttggtagg cggtatgaaa taccatcatc gagatcttcg gaatgtgctt
    26941 gaacggtcct ctgcaagaga gacgaccatg cgtgcagcaa tcggcgcaat tgccaagaaa
    27001 attctggaag aacttgacat catcgtagca ggacacgtga tttgtcttgg tggaatccaa
    27061 gcagaaatac ctggaaatat ctcgattcaa gaaatcaaag aaatttctga agcgtcagaa
    27121 gtacgagttg ttgatccagc agttgagcaa agaataaaag atttgatcga tcagacaaag
    27181 aaaaatggag atacgatagg aggaattgta gaagtgctcg ttggaggtgt tccagctgga
    27241 ttaggaagct acgtacaatg ggacacgaag ctagatgcca aaatcgcaca agctgtggtt
    27301 agtatcaatg cttttaaagg cgtagaattt ggggtcggat tcacttctgg ttcattacca
    27361 ggctcacaag tgatggatga gatcctatgg gaagaagaaa gaggctattt ccgacgtacc
    27421 aataatctag gcggcttcga aggcggaatg accaatggcg aaccacttgt cattcgcggg
    27481 gtcatgaaac ccatccctac attgtacaag ccacttcaaa gtattgatat cgatacaaaa
    27541 gaaccttaca aagcaagtgt agaacgttcg gatagtacag cagttcctgc agctagcgtc
    27601 gttgcggaaa atgttgtagc cactgtggta gcgacagagc tattgtcaaa attcccaagt
    27661 gattcgcttg ttgagttgaa agttgcagtg aaggagtacc gtgactactt gaaacattat
    27721 taaaaagggg gcaaataaga tggcacaaca tgtttttatc gtggggcttg gactgattgg
    27781 tgcttctatg gcactttgta ttcgtcagac caatccagaa ctgatagtag ttggttggga
    27841 tagtcagtca aaaacccgtg agcaagcagt agaacagcag atcgttgatt acgttgcaag
    27901 tgattttgaa acaggtgcag aacaagcaga cgttatctta ttagccgttc ccgtccgtac
    27961 aaccttagct tatcttgatg tccttgaaag aaccaacttg tctgatcacg tcatcattac
    28021 ggatgtcagc agtacgaagc agcaagtcgt gacatccgct gagcagaaaa atcttcggtt
    28081 tgtcggtgga catccaatgg ctggttctca taaatcaggt atacaggcag cagatgctaa
    28141 actatttgaa aatgcctatt acatctttac acctttggaa aagacgaaaa aagatgttga
    28201 aaaactgcaa gaactatttt gcggaaccaa agcaaaatat gtaactctta cagcaagaga
    28261 acatgaccgt ataacaggaa tgctaagtca ttttccacat attctggcag ctggcttagt
    28321 caatcaggca gaacaattca atcaagagta tccgcgagcc aaacaactgg cagcaggagg
    28381 ctttcgagat atcactagga tcgcttcctc ggatcctgtt atgtggacag atattttgtt
    28441 gagcaataag cagatattat tggaacgatt ggcggattgg cagcaagaaa tggcacaaat
    28501 agcagaatgg atcatgactg agaaccaatc ggaaatcttt tcttttttca atcgggcaaa
    28561 agaaagtcga gaccagttgc ctatccataa gcaaggcgcg attcctgcct tttttgattt
    28621 atttatcgat gtgccagatg aacctggcgt gattgcagaa gtaactggac tgatcggaaa
    28681 agcgggagtt tctttgatta atttgaagat ccaagagacc agggaagata ttttaggaat
    28741 actgcaaatc agttttaaaa accaacagga tttgttacaa gcaaaaagaa tcattatgtc
    28801 taataccagt taccattgct ggatcaaata ggaggctgga taaatgcaat tattacaaca
    28861 aatacatgga ttaagaggaa ctgttaggat accagcagac aaatcaattt ctcatcgcag
    28921 catcatgttt ggggcaattg cggaaggaac gacgactata caaaattttt tgcgcgcaga
    28981 agattgtctg agtactttac atgccttcca gcaattaggc gttgaaatcg aagaagagga
    29041 agagatgatc aagattcatg gtcgcggtag ccactcattt gtccaaccaa ccgaatcaat
    29101 cgacatggga aactccggta cgaccatacg tttattgatg ggtattttgg ctggacagcc
    29161 ttttacgact actctgttcg gggatgcttc gttgtctaaa cgtccaatgg ggcgagtgat
    29221 ggaaccttta cgcgagatgg gcgctgactt gcaaggaaaa gagaatgagc agtatctacc
    29281 aatcactgtg acaggaacca actctttatc acctatccga tacaatatgc ctgtagccag
    29341 tgcgcaagtc aaatctgctt tgctgtttgc ggcattacaa gcagaaggca catccgtaat
    29401 cgttgaaaaa gaacgttccc gtaaccatac ggaagaaatg attcgtcaat ttggcggaag
    29461 gatctcagta gaaggtaaaa caatcacggt aacaggacca caaaaattaa ctggtcaaca
    29521 gataactgtt ccaggcgata tttcatcggc tgctttcttt ctagcagcag ggcttcttgt
    29581 tccggaaagc caactgttat taaaaaatgt cggggtcaat ccaacaagaa ccggtatctt
    29641 agatgtgctg gaggagatgg gcgcagcgat tacccatacg aatcacaatg aacagaacca
    29701 atcggctgat ttaagtgtca agacgagtca tttgaagaaa gcaacgattg gtggagaaat
    29761 cattccacgt ctgattgatg aattgcccat tctagcttta gtggctactc aagcagaagg
    29821 aatcacgatc atcaaagacg cagaagaatt aaaagtaaaa gaaaccaaca gaattgatgc
    29881 ggtggcagaa gaattacaga aaatgggagc aaagataaaa gcgacagcag acggtctgat
    29941 catccatggg ccgacacctc tgcagggcgc aaaagtgtcc agcagaggcg atcatcgaat
    30001 cggtatgatg ctgcaagtag ctgctttgat agctgatgga ccttgcgaac tagaaggagc
    30061 tgaagctgtt tcaatctctt atccggcatt ttttgacgat ttagcagaac ttgtgtcagg
    30121 aggagaagct catggctagc attttgctga ttggtttcat gggcgctgga aaaacaacaa
    30181 tcggtaaagg gttagctcaa cgtttacaaa aagagtatgt agatttagat acaaagattg
    30241 aggaacatat ccaattgtcg attgccgaat attttcacta ttacggtgaa aaaagctttc
    30301 gaaaagtgga gtcagatatt ttaagaaagc tgtcaaatga agataaaatt atcgcgactg
    30361 gtggagggat tgtccaaagt gcagaaaatc ggcgtttttt aaaaacacag ccgattgttc
    30421 tttacttaga ggcagaagcg gactgtctag ttgatcgtat ccaacaagat gaaacgtcta
    30481 ttcgaccttt agctcttgga aaaacaagag aagaaataaa agcattattg gcacaacgac
    30541 tttcttggta tgaagaaagt gcgacacatc gaatcaacac gaccaatcaa acaccagaaa
    30601 agatcatcga taccattata gaaaggatca aaagcatatg aacgtcagct atctagggcc
    30661 agaaagttca ttcacttatc aggcggcttg tcagctattt cctacagaac aattgactgc
    30721 gtatgcttcc atccctgctt gtttgcgcgc tttgtttcgc aaacaagtcg atctggctgt
    30781 cgttcctgta gagaattcat tggaaggatc tgttcatcac acaatcgatc ttctttccaa
    30841 acatccagaa gtcgaagtta aaagtgaaat cgttcttcct atcaagcagc agcttttagg
    30901 caatccagca acaaagatta caaaaatcct ttcacatcca caagcactag cacaatcaca
    30961 acagttttta gaaacgcatt atcctaatgt tcctttagta gcaacagaat caacaactgc
    31021 tgcagcaatg tatgtagcag aacatccgaa agaagacgca gcagcgattg cttcattaga
    31081 aacagcacag catgttggat tggagatctt agctgagaat atccaagata atgagttgaa
    31141 tcagacacgt ttttggatca tagagatcac cagccagcag tcagctccag taaaaatgag
    31201 tgtcatcttg acactacctg ctaatcggcc agggatgctt cataaaatgc tggcagcttt
    31261 tggctggcgg gaaatcaact taagtaagat tgaatcccga ccattgaaaa ctagcttggg
    31321 agaatatttt ttcgtgatcg atttgttgtt ggaccgccca atgacattag tagagaatgc
    31381 gctccaagaa atcaagatgc tcggaggaga aagtcaaata ttgggctgtt atccagtgct
    31441 gacagtagaa gagacgcgta gatgaaagtc aagaatactg attgatttat ttaacttaaa
    31501 ataaaaaaat cagcgcttgg ctattcttta tttttgatat tctgattgat ttttaggaag
    31561 aagcggtaga caaatcgtta cagtcgttcc tgtcccaatc actgattgga tcgttaagcc
    31621 atatgcatcg ccatacatta ggcgtatccg ctcatctaca tggctgatgc cgattcctga
    31681 aaaatgatca tttttttgtt tggtattttc agtatcgaag ccatcgccgt tatcaatcac
    31741 ttcgaataaa agaagctgat tttttgtttt gccgtacacc gtgatgaatc cttgcgtttt
    31801 attgtgaaaa gcatgaagat aggcgttttc gataattggt tgaaggatca tattcggcat
    31861 agataatgac tgcagttcat caggaatcat tattttagcc gagattcttt cgccatatcg
    31921 catactcaaa agatcgatgt aactttgtac gcttttgatt tcttccttga ctggaatcac
    31981 ttcttctttt ttcccaatcg tacttcttag taaagtaatg aaactgtcga ttccttgaac
    32041 tgcttcatct gtttttttct gccaaatcaa aaatttcaag ctagtcagcg tattataaat
    32101 aaaatggggc tgtatctgtg cttgcatagt ttggatttca agcgaacgtt tcttcttttc
    32161 gtcttggatc gtctgttcat aatgatgctg agcattactt agcaattgat tgatcgtatt
    32221 ccctagcaat tggacttcat ggcttccttg tacaggaaca gtagctttta agccatcttc
    32281 ctctagccgt tttagcgaat caatcaagcg gtagatcgat tttgtgtgtc gatccaaata
    32341 cataaatacc aatgagccag caaaagcaag tgtcacgata ttatacaaaa taatcggaaa
    32401 aatcacgccc atttggttag acaaagaatg aagatcgact tgactgagta atgtacaatc
    32461 ctggcggtac aaaggaagac gggtcaactg ctgtccattt gaaatcgttg tttcacctac
    32521 tgttagtttt ttgaaaatca ttgatcggct cccaattgta ttttcttcat ttgatgcgat
    32581 gatcgtctgt tcttcgttca tgaaggagat cttcgatatc tcaggattta ctacagattg
    32641 aaaaagattc gctagttgct tggatgagat cgataaaaca gcatatccgt aaacgtgatt
    32701 caatgtattt ttcagttttt tgacaaagaa gacgctgggt gctttcttat cgcgaagagt
    32761 caatccgtgg tgtgcaaaaa gcatttgtgt atcagcagca cgttcattca ctctctgagc
    32821 aatcgcagat tgaaaaaaag aatcggcaga tacggcagtg agcatgtcgt ttccgacaaa
    32881 agttcttcca ttttctccaa agatcaatac atcgtaatcg atcattggat ggatttttga
    32941 aagagagtca ttcatttttt ggattgttct tagttgctga agctgttcca tatgttgggc
    33001 tggatcattg gaaaaatagc tttcgacagc tggattagaa tcaaaagcgg ctaatgtatt
    33061 ctcgattgta tcattcaagc tttcaaattc agattggatc ttttttagta ccacatcatt
    33121 cgattgattg aacagctgga tataggcatc ccttgatcga tgataaataa acaaagagat
    33181 gacgatggct tgcagaaaaa gaccgatgaa caaaatgaac agaagtttgg taaagagttc
    33241 atgctctttg attttttttc tcattttaaa aaagttcctt tacggtattg agaaggtgtc
    33301 attcctgtct cttctttaaa aactctggag aaataaccca gttcggtata gccgactttc
    33361 tcacagattt ccgtcaaagt aagccgtgtt tccgtcaaaa agagcttggc tttactgata
    33421 cggacttttt ttagatattt tgaaaagttg ataccaaatt tttcagtaaa gatggctgag
    33481 agatagctat atgaaaaatg atatttttcg gataaaagtt gtaaattgat ttctttctca
    33541 taattttcct ggacgaactc gtagatcaaa gtcaagatat ctaaattgtc tttttttgca
    33601 tttgcagaaa agtgggtgag tttctcaagt gtttctttga caagaataga aaaatcttcg
    33661 aaatactcag catcggctaa taaagaagga agggcgatct tgagcaaaga aagttcttca
    33721 ttcccaacat gctcgtcagc caaagcgttg agtaatgcat agaaacgatt gattgcctca
    33781 tgtttcaaat aagctggatc aataggctgt tccaatatcg tcgtgaagaa attctcaagt
    33841 tctttgacag ctaaagcgta gtctttatga acgatttttg ttaaataact acttgcttgc
    33901 tgctcgtggt tggttaataa cggaagaagt tcatgctgat aagcaaggtt ctttccctta
    33961 tgatagaaac gttgtccttt ggtgcaattt ctgatctctt ggaatatttc attgatatct
    34021 tggattgcaa aagaatcaga aacaacaaaa aaatcacctg gaaaaacttc ttgacgaaac
    34081 ttttgaacaa acttcctaag tgtatctttg gaatcagtat tgattacata gccggattct
    34141 tccgaaaagg tagaaacagg ataaatagta aacgaatcat cgctatccat caatgaatgg
    34201 gtgatcgact atttttgttt agagccttgt tttgaactga atggccgttt ttcggtatat
    34261 aacagaaaaa acagggtgtg ttcaaaaaga agagggtcaa gcaatggacg agtttgtcca
    34321 gcaatctgct gtgaaagatt ctcgcagatc tgctcataac gattggtgat tgggcttttg
    34381 ttgggctttt ttttgatttt ttgtgaaacg gcagttaagg aatttagaaa aaaatcagga
    34441 ctcaatcgag gctttaaaag ataatcagcg acgccttctt taaaagaatc tttaaccata
    34501 tcaaattcat catagctaga gaggataagg atctgtgttt caggttgttg ctgttttaat
    34561 agccgcgata attcgattcc atttaattca ggcatcatta catctgtgac cacgatatcg
    34621 attgcatgta ccaaagaaaa atcaagcgct ttcaatgggc tggaaaaagt ccctattaat
    34681 tgaaacccgt gctcagacca attgatcaac tgtttcattc catctaaaat cgtctgttca
    34741 tcatcgataa gaattacgcg tattggcatt tttttacccg ccttaagaat agtcttataa
    34801 tttaaaaata cttatttcta ggagaaaatg caaggatatt tttattttaa agaaacctca
    34861 ccat
//
LOCUS       DANKHK010000018        34489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000018 DANKHK010000000
VERSION     DANKHK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            212..604
                     /locus_tag="PW109_000796"
     CDS             212..604
                     /locus_tag="PW109_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2510663.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PW109_000797"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PW109_000797"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2510664.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PW109_000798"
     CDS             3165..4775
                     /locus_tag="PW109_000798"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2510665.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PW109_000799"
     CDS             5067..5936
                     /locus_tag="PW109_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2510666.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PW109_000800"
     CDS             6101..7372
                     /locus_tag="PW109_000800"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2510667.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PW109_000801"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PW109_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2510668.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PW109_000802"
     CDS             8763..9026
                     /locus_tag="PW109_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2510669.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PW109_000803"
     CDS             9471..9920
                     /locus_tag="PW109_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2510670.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PW109_000804"
     CDS             9977..11395
                     /locus_tag="PW109_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2510671.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PW109_000805"
     CDS             11395..12255
                     /locus_tag="PW109_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2510672.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PW109_000806"
     CDS             12429..13202
                     /locus_tag="PW109_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2510673.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PW109_000807"
     CDS             13349..13714
                     /locus_tag="PW109_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2510674.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PW109_000808"
     CDS             complement(13862..15070)
                     /locus_tag="PW109_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2510675.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PW109_000809"
     CDS             complement(15089..15367)
                     /locus_tag="PW109_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510676.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PW109_000810"
     CDS             15521..16216
                     /locus_tag="PW109_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2510677.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PW109_000811"
     CDS             complement(16658..17455)
                     /locus_tag="PW109_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2510678.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PW109_000812"
     CDS             complement(17452..18636)
                     /locus_tag="PW109_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2510679.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PW109_000813"
     CDS             complement(18661..19908)
                     /locus_tag="PW109_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2510680.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PW109_000814"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PW109_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2510681.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PW109_000815"
     CDS             21888..22202
                     /locus_tag="PW109_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2510682.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PW109_000816"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PW109_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2510683.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PW109_000817"
     CDS             22887..23531
                     /locus_tag="PW109_000817"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2510684.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PW109_000818"
     CDS             23546..23875
                     /locus_tag="PW109_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2510685.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PW109_000819"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PW109_000819"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2510686.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PW109_000820"
     CDS             24863..25687
                     /locus_tag="PW109_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2510687.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PW109_000821"
     CDS             25680..26027
                     /locus_tag="PW109_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2510688.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PW109_000822"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PW109_000822"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2510689.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PW109_000823"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PW109_000823"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2510690.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PW109_000824"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PW109_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2510691.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PW109_000825"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PW109_000825"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2510692.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PW109_000826"
     CDS             31657..32451
                     /locus_tag="PW109_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510693.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PW109_000827"
     CDS             32441..34483
                     /locus_tag="PW109_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510694.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11080 a         6118 c         7246 g        10045 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatgtcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacg
//
LOCUS       DANKHK010000019        34211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000019 DANKHK010000000
VERSION     DANKHK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            66..1022
                     /locus_tag="PW109_000828"
     CDS             66..1022
                     /locus_tag="PW109_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2510695.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1095..1211
                     /locus_tag="PW109_000829"
     CDS             1095..1211
                     /locus_tag="PW109_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2510696.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1266..2948)
                     /gene="recN"
                     /locus_tag="PW109_000830"
     CDS             complement(1266..2948)
                     /gene="recN"
                     /locus_tag="PW109_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2510697.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2966..3415)
                     /locus_tag="PW109_000831"
     CDS             complement(2966..3415)
                     /locus_tag="PW109_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2510698.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3522..4340)
                     /locus_tag="PW109_000832"
     CDS             complement(3522..4340)
                     /locus_tag="PW109_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2510699.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4349..5239)
                     /locus_tag="PW109_000833"
     CDS             complement(4349..5239)
                     /locus_tag="PW109_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2510700.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMINQFDVEK"
     gene            complement(5241..5465)
                     /locus_tag="PW109_000834"
     CDS             complement(5241..5465)
                     /locus_tag="PW109_000834"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2510701.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5470..6807)
                     /locus_tag="PW109_000835"
     CDS             complement(5470..6807)
                     /locus_tag="PW109_000835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2510702.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6808..7662)
                     /locus_tag="PW109_000836"
     CDS             complement(6808..7662)
                     /locus_tag="PW109_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2510703.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7759..8208)
                     /gene="nusB"
                     /locus_tag="PW109_000837"
     CDS             complement(7759..8208)
                     /gene="nusB"
                     /locus_tag="PW109_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2510704.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8201..8626)
                     /locus_tag="PW109_000838"
     CDS             complement(8201..8626)
                     /locus_tag="PW109_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2510705.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8872..9936)
                     /locus_tag="PW109_000839"
     CDS             complement(8872..9936)
                     /locus_tag="PW109_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2510706.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10108..10353)
                     /locus_tag="PW109_000840"
     CDS             complement(10108..10353)
                     /locus_tag="PW109_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2510707.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10492..10866)
                     /locus_tag="PW109_000841"
     CDS             complement(10492..10866)
                     /locus_tag="PW109_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2510708.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10949..11863)
                     /locus_tag="PW109_000842"
     CDS             complement(10949..11863)
                     /locus_tag="PW109_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2510709.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11884..12687)
                     /locus_tag="PW109_000843"
     CDS             complement(11884..12687)
                     /locus_tag="PW109_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2510710.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12720..13709)
                     /gene="manX"
                     /locus_tag="PW109_000844"
     CDS             complement(12720..13709)
                     /gene="manX"
                     /locus_tag="PW109_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2510711.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13812..14303)
                     /locus_tag="PW109_000845"
     CDS             complement(13812..14303)
                     /locus_tag="PW109_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2510712.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14494..17301)
                     /locus_tag="PW109_000846"
     CDS             complement(14494..17301)
                     /locus_tag="PW109_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2510713.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17735..18028)
                     /gene="rpmA"
                     /locus_tag="PW109_000847"
     CDS             complement(17735..18028)
                     /gene="rpmA"
                     /locus_tag="PW109_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2510714.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18041..18385)
                     /locus_tag="PW109_000848"
     CDS             complement(18041..18385)
                     /locus_tag="PW109_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2510715.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18412..18720)
                     /gene="rplU"
                     /locus_tag="PW109_000849"
     CDS             complement(18412..18720)
                     /gene="rplU"
                     /locus_tag="PW109_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2510716.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(18740..18816)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19002..19643
                     /locus_tag="PW109_000850"
     CDS             19002..19643
                     /locus_tag="PW109_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2510717.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19705..21336)
                     /locus_tag="PW109_000851"
     CDS             complement(19705..21336)
                     /locus_tag="PW109_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2510718.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21459..21818)
                     /locus_tag="PW109_000852"
     CDS             complement(21459..21818)
                     /locus_tag="PW109_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2510719.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21977..22050)
                     /locus_tag="PW109_000853"
     tRNA            complement(21977..22050)
                     /locus_tag="PW109_000853"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22014..22016),aa:Arg,seq:tct)
     gene            complement(22359..23042)
                     /locus_tag="PW109_000854"
     CDS             complement(22359..23042)
                     /locus_tag="PW109_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2510720.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23170..23688)
                     /locus_tag="PW109_000855"
     CDS             complement(23170..23688)
                     /locus_tag="PW109_000855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2510721.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            23900..25744
                     /locus_tag="PW109_000856"
     CDS             23900..25744
                     /locus_tag="PW109_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510722.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            complement(25789..26793)
                     /locus_tag="PW109_000857"
     CDS             complement(25789..26793)
                     /locus_tag="PW109_000857"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2510723.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26804..27544)
                     /locus_tag="PW109_000858"
     CDS             complement(26804..27544)
                     /locus_tag="PW109_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2510724.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            complement(27614..28417)
                     /locus_tag="PW109_000859"
     CDS             complement(27614..28417)
                     /locus_tag="PW109_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2510725.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            complement(28417..29499)
                     /locus_tag="PW109_000860"
     CDS             complement(28417..29499)
                     /locus_tag="PW109_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2510726.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(29640..30908)
                     /gene="dltD"
                     /locus_tag="PW109_000861"
     CDS             complement(29640..30908)
                     /gene="dltD"
                     /locus_tag="PW109_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2510727.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(30911..31144)
                     /gene="dltC"
                     /locus_tag="PW109_000862"
     CDS             complement(30911..31144)
                     /gene="dltC"
                     /locus_tag="PW109_000862"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2510728.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(31189..32394)
                     /gene="dltB"
                     /locus_tag="PW109_000863"
     CDS             complement(31189..32394)
                     /gene="dltB"
                     /locus_tag="PW109_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2510729.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(32391..33905)
                     /gene="dltA"
                     /locus_tag="PW109_000864"
     CDS             complement(32391..33905)
                     /gene="dltA"
                     /locus_tag="PW109_000864"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2510730.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(33916..34065)
                     /locus_tag="PW109_000865"
     CDS             complement(33916..34065)
                     /locus_tag="PW109_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2510731.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT        10155 a         7318 c         5651 g        11087 t
ORIGIN      
        1 cacattccat gtattccaat tccctatccg ttataataaa agaaacaggc aacaggagga
       61 aaatcatgat caaatatttt acattagata atgagaaact gcgtaatacc ggaaaggaaa
      121 ctgaccaaac gatctggtat tgtgtagaac gtcctaatca agaagagatt catcagctga
      181 cacatcaatt tgaaattccg tcagattaca tcacttccat attggacgat gcagaaaatt
      241 cccgtacaga agaattcaat caatcaaagt tgacaaagcc agcgttatta ttgttacaat
      301 atccttttgc taaaacaagt cctagcggtt attttcaagt ggatacatac ccgctctcta
      361 ttattttgac gcccaaaaag aagcttatca caattacgaa tcatgaacca ctgtttttaa
      421 agaaagtctt tagccagacc tttccaaaaa gtgatatttc gcctaatctc aatatcttct
      481 ttcaattcat ttggcaggtc acactctctt acaacagtta tttagctacg cttcaatctc
      541 aatgcgatac tttggaagcc aaacttcaag tatccactga aaacaaacaa ctttatcaaa
      601 tcatggatat ccaaaaaagc ctcgtttatt tcagagaagc gacttctgct aatttagatg
      661 cattgaaaga actagcgcaa aataaacttt tccaagaaaa tcatgctttg aaaaatcatt
      721 tacgtgatgt attggtggaa acagagcagg caatgactac ttctcgcatt caattaaaac
      781 tagtagaaca aatgaatcag actttttcag caatcgtatc gaataatctg aataatatta
      841 tgaaaatttt aacttcttta acgattattt taactatacc gacaatcgtc ggcagtatct
      901 atggaatgaa cattaagtta cctattgctg aaagggatga tgcctttata tggctagttc
      961 tattaacatt atttatttgt atcgtaacta cctattattt aaaaaaaggg aagtttttat
     1021 aaaaaagccc tcttgtggaa atggaaaaaa gattgtacac ttgaatcaac taattaatga
     1081 cgaggttgaa aacaatgcgt gaaaaaaaac aaacaagtac ttttgctaaa gtaacaaaat
     1141 ttgtcatttg gacaatgctg attttaacga ttggatctgt tgtattgact gctgtaatga
     1201 gtgtcatgta aaaataaaat atagctatca gctgctgaaa aggacctgaa ccactgcgag
     1261 gtggctcagg tcctttttgc gagtttcaat agttcttttg catgttcgag tgtgagtttt
     1321 gtaatctcac ttccagccag catacgtgca atttcttctt ctctttcttt tgttttcaac
     1381 tctgaaacag aagtttgcgt acgtccatca atcactgctt tcacaatata atattgatag
     1441 tcggcgactg cagcaacttg aggcagatga gtgatgcaca aaacttgaga gtatttcgat
     1501 atttgagaaa ttttatctgc gatggcctgt gcaactcttc cgctcacacc tgtatccact
     1561 tcatcaaaaa taatacttgt cacaccttgc tcagaagaaa aaatagtttt caaagcaagc
     1621 aacatccgag acagttcgcc accagaagcg actttcacca aaggcttcag tggctctcct
     1681 ggattggtcg taatgtaaaa ttcgatttgg tcaaatccat catgattcaa ttgtctattt
     1741 tcttctttta agaaacgtac ctcaaactca gtgttttcca tatacagact tttcaattca
     1801 tgcagaatgg atttttccaa ttcttttgcg atttttttac gcgcttcatg aagatctgcg
     1861 gcttgttgct gaagtagaag ctctttttgc tctagttcct ctttcatttt gtccaattga
     1921 ccttctgaaa aatcagaaga agctagctct tcggtgattt cgtcatagta agccaatatt
     1981 gactcaatgg attcgccgta tttacgtttc aactgtcgga ttaattctaa acgttgcgta
     2041 acttcttcta aacgactctc atctaactcc aataaatcaa tctgacgact catatcgccg
     2101 attgcatctt gcagcaaata gtaagcactt tggatatttt cataaattgc ttcatactcc
     2161 acatctagat gggcgatccc ttgcagttct gaaacggcca agccaacacc atctacactg
     2221 ttttgttcat tattcgtcag tgctccgtaa cctgcagcta atccatcagc aattttttga
     2281 taattgctaa gtttttctct ttcttctttt aatttctctt cttctccgac ttgcaaatca
     2341 gcagcagcta tttcttcttg ctggaaatgg agcatatcta ttctctgaac ataggatttt
     2401 tcattttgct gcacttttcg tacccttttt tccagctcac gataagagat atattcttgc
     2461 tgataactct ttttcttctg ttggaaagct acatctccaa aacgatccaa taaagcaaga
     2521 tgtgattcag gctggagcag ttcctgatgt tcgttttgtc cttggatatc tactaaataa
     2581 ctgccgattc ttctcagatt agctaatgtg ataatatgac cattcactcg acaaacattt
     2641 tttccagtta aggacatatc ccgtcgaaca atcagattgt cctcatctgt ttcgatccct
     2701 aattcaacca tcagttcaga gaatccttct tgttttggta gttcaaacaa cccttcaagc
     2761 acacattttt ctgctccttg acgtatataa tcgcttgaac ctcttcctcc tgctaacaga
     2821 cctaaagcat cgataataat ggatttaccc gcaccagtct ctccggtcag cgcggtcatt
     2881 ccttcgtgaa aagaaagccg caattccgga ataatcgcaa agttagtaat actgatttct
     2941 tgtagcatca cattcgctcc tcaacttaaa ggtaatgcag gaagtctttc ttcaataaaa
     3001 ctgcctggtc ttcagttcgt gtaatcatca aaacattatc gtcatcattg ataatcgaaa
     3061 ataattcttt cttgtaacgt ttatcaatca aatttgctgc tgccgaagca tttcccggca
     3121 atgttttcaa aataacaaat ttttccattt ggtcaactgc cacgaaagcg tcttttaata
     3181 gtttttccaa tttagtattt acatcttcgt ttgtttcagc tggtatgcta taacggtagc
     3241 cgccatctga agccggtacc ttgatcagtt tcagttcctt tatatctctg gaaatcgtag
     3301 cttgagtaac agatattcct ttgcttttca ggatttcaac aaattcttcc tgctttcgga
     3361 tatcttgctc actcaacaat cgagtaatca aacgatgccg atcttttttt ctcattacgt
     3421 cttcgctcct tagcatattc ttcttaatca atgatagctt atttccttgg tataataaag
     3481 aagaatccgc atacttctca cttgattttc acagttacaa tttatttatg gtcaaactgt
     3541 tcgtgtgcat ttgaaaccac ttctgccata ttaatagctg aattgattgt tccgctttcg
     3601 ggtactttcc tcaagtgagc caagaactcg atatttcctt ctccccccgt gatcggagaa
     3661 taacttaatt ccagcacatc atatccacct tgtgccgcaa atgacaaaat gtcttctaaa
     3721 actttttgat gggtctgagg gtcacgaaca attccgtttt tccctacagc ttctctgcct
     3781 gcttcgaatt ggggtttgat taaagcaatc acttctccct cttcttttaa aatagcatgt
     3841 aatggaggca gcatcagttt tagtgaaata aaagaaacat cgataacagc aacttctgga
     3901 attcctaagg taaagtcttc gggttttgca taacgaaaat tcactctttc cattacgacg
     3961 actctaggat cttgtcttat tttccaagct agctgattgt agccaacgtc taaagcataa
     4021 ctcatttttg cgccattttg caaagctgca tcagtaaatc cgccagtgga tgcaccaata
     4081 tctaacaatg tttttccttc aacagataga tcaaatgctt tgagtgcttt ttctaatttt
     4141 aatccccctc gagaaacata aggaagtttt ttacctttga ctcttaattc actactcact
     4201 ggaatttttt ctcccggttt atcaaaccgc tcgttttttt cgttgtaaat caaaccagcc
     4261 atcactgaac gtttggcttg ttctcttgtt tcaaataatc cttgtttgac cgctaataca
     4321 tctacgcgtt ctttttccat tctttccctc atttctccac atcaaattgg ttgatcatct
     4381 ggcgcatcag cccgccatca aaagaaaaaa cgtcctcttc taatttgtct atgatcttgt
     4441 tggcggataa cagctcaatc tccaacgcct cacgtgtctt ttctaggcct aataaacgcg
     4501 ggtaagtatt tttttccata cgttcatctt taccagctgt tttccctaat gttttcgttg
     4561 taccaatcac atcgagtaga tcatctctta tctgaaaagc taaacctaaa tgttcggcta
     4621 atcgttgaag tagcaataag atttcttcag gttgcttcgc taatatgcca ccagccagca
     4681 aggcataacg aatcaaatgc cctgtctttc tttcatgaat aaatgctaat tcttctaaag
     4741 aaagcttttt cgattcgccc tcgatatctg cagcttgccc agcaaccata ccttgtgatc
     4801 ccgcacaaac agctaattgc tgaagcagca gaagcttagg agaattacct aagtgtgcca
     4861 tactgattag ctggaatgct cctgtaagca aaccatctcc ggccaatata gctaatgctt
     4921 cgccgaatac tttatgattg gtaggttttc ctctacgaag atcatcattg tccatcgcag
     4981 gcaaatcatc gtggatcaag gaatacgtat gcaccatttc taaagcagct gctacttgat
     5041 agacgggaac atcaataggc tcatcaaagg aagcaacggc cgttaaaagt aataagggac
     5101 gtattctttt cccgcctgct ttcactgaat aactcatcgc atcatagagt cctctatccg
     5161 ttgtatgctc atctaaaaaa gacaagattt ctttttctac agcaggtaga tgaatagctg
     5221 aaaagtcagt taatctcatc tcagctttcc tcgtttccgt caaaatcaac ttcttcattt
     5281 gattctgtca tcattttcgt caaggtctct tctgctttag ctaatgtatc ttggcaatgt
     5341 ttgcttaatt ccattcctcg tttaaacgaa tctagagctg attctaatgg aacgtctcct
     5401 tgttctaact gcatgacaat tttttctaat tcttgcagtg actcttcaaa agttggattt
     5461 tccatatttt tattctcctt tgtctatctc ttgtacttgt cctttaacga ttccatcttg
     5521 ataatggatc gtcagcaaat ccccttgatg aatctcggtg acacttttta ctacttgatc
     5581 atttaacgtc gtatagctgt agcctcttcc catgattttc aatggactca acaaatcaag
     5641 agattgaaca gcttgagcaa aacgctgctg ctgttgtttc atatattgtt ggattttctc
     5701 ttctaagcgt ttttctagat aagcagtttc ttggcgagct gctttaactc gataaagcgg
     5761 agccacttgt tccaattggt ggcttaatgc gatcgtttgt ttttctttcg catgataaat
     5821 ttgctgtgta gcttgatata atcgctgttt caactggtct aatttgatgg tctgaccttc
     5881 atacaatctt tcaggctgcc gaaatacata ggaacgtcga gcacgctcga accgttcttg
     5941 tttgcgctgg atctggcgta caaacgcctg ctgcaatcta gcttgccgtt cttgaatgcg
     6001 catcaactct tctgacaaaa caggaacagc taattctgca gctgctgttg gagtggcagc
     6061 ccgaacatct gctactaaat cagcgatcgt ggtatctgtt tcatgtccta cagatgaaat
     6121 caccggcgta ttagcttgat aaatcgcccg agcaacccgc tcttcattaa acggccataa
     6181 atcctcaatc gaaccacccc cacgaccgat gatcattgta tcgaaattgc ccatctcttc
     6241 tactctctgg atattcctga caacattgtc cgctgcttgg tttccttgta caactgttgg
     6301 gaacaaaacc aattggacaa ttggatatct tcttttcacc gtcgtgatga tatcgcggat
     6361 aaccgctccg cttggactgg taaggaccgc gattcgcttt gggaaacgtg gcagcatttt
     6421 tttaggccct tgaaaaaggc cttccttcat cagcttttct tttcgttctt ccaattcttg
     6481 atataaagca cctattccat caggctccat gtgctctaca tagatttgat aagaacctcc
     6541 cgcttcatat aatgaaatac gcccaacgac aaggactttc attccttctt ttggctggaa
     6601 tcgtaatttt tggaatgctc ctttgaacat aatagcagat atttttgcat ggtcatcttt
     6661 taagctgaaa tattgatggg cattggcacg taaacggaaa ttcgatattt cgcctgttaa
     6721 atatacgcgt tctaaataag gatcggcatc aaattttctt ttcaaatact tcgttaatgt
     6781 agtgacagtt aaatattctt gtgccatcta ttcacctgct ccaaaagctt cggcagattt
     6841 tacggtttga tacatcagca tcgtgatagt cattggccct acaccctttg gcacaggagt
     6901 gatccagcct gcaataggct ccacttcatc aaatttcaca tcaccgatca gcttaccgtt
     6961 agcatcacga ttcatcccta catctatcac tacagcacct ggttttacga attcttttgt
     7021 gacaaaatgt ccacgtccta ttgctacgac taaaatatct gcttcttgtg ccagttcagc
     7081 cagattttcg gttttagaat gagcaacagt aacagttgca tctgccatca agagtagctg
     7141 agccatcggt ttaccgacga tattgcttcg tccgataact actgctcgtt tacccgccgg
     7201 atcgatagaa taagcttcaa acattttcat gatgccgtat ggagtacaag gaatcttgtc
     7261 cggtttaccc gcaaggagtc tgcctaaatt catcggatga aaaccgtcta cgtctttttc
     7321 agggcggata gctaacagga tcttttcttc atctatatgc tttggcaatg gtaattggac
     7381 caatatacca tgaaaactag gatcttgatt gtatttttca atttctgcaa gtagttcgtc
     7441 ttcagaaatc gtctcaggat aacgctctac caatgaatgg attccgattt cttttgctac
     7501 acgttcttta tttcttacat agatttggct ggctggattt tcccctacca ataaaacgac
     7561 taatccggga tggataccat tatctttcaa tttttggatt ttttcagcaa tctctgcctg
     7621 cattttttca gctaattctt ttccgttaat caattctgcc atctaaccac tcccagatat
     7681 tttttgtgtt ttcattctac catagaattt tcccgattta taacaggttt atgatgattc
     7741 atagaaaaaa gctaggactt acgccccagc ttctaattct ttcaatacat tggataaaac
     7801 accgttgacg aatttacgtg aacggtcatc actaaatgtt tttgccaact cgattgcttc
     7861 atttaaagca acagccgctg gtacatctgt cacataagtc atttcaaaaa tcgcgatacg
     7921 caagatcacc aaatccattt ttgagatccg attgatgtgc caattgtttt ttaaatgttt
     7981 ttcaatcact ttatccaatt catctttttt tgcacagaca ccgccaacta gcgtatcaag
     8041 ataaacagga acaaactctg attcatcttc gttgatcatg tcgcggtggt ctaattcgat
     8101 tgcgttgaaa atcgcatctt cttttgttag gtctgcattg aagtctaatg gaaataaagc
     8161 ctgcaatgcc atttcccgaa tctcgtgtct tgataattct ttattcattc tcttcctctt
     8221 cgtcttcaaa aagttcttcg attgttggtt gttctaattt ttcaggaaca acagctacta
     8281 catggatatt gacttcagat aaatcgatat ccgtcatgaa aaggacttgc tggctgaccc
     8341 gttcctgcat ttccattgct actttaggaa ctgaaacacc atattctaga taacagtaaa
     8401 gatctacttt caagccatct tcctcgtttg ctaagtaaac accttttccg tgagctgcac
     8461 gtcctaataa ttcagtgaca ttgctggcaa aagttccgcg cattccgtat acgccttcta
     8521 ctttagaagc tgcaatcccg ataattactt cgatgacttc aggagcgatc acgatctctc
     8581 ctaaatcttg gtttgcgttt aaaatcaaat ttttttcatc tgccatttat ttattcctcc
     8641 ctttgcctaa gcacgagata catccgaatt ctctatgatg cggcatagtg atcacgtggt
     8701 aagaaaactg ccgccgatca gctgcaaact gctatgaatt ggagaaaaca cccaacttta
     8761 ttatctctta cagcttaaaa gttcttctat agtgtatcat taaatcgagt gattttctat
     8821 aacaaagtct gtgaacactg atttttacgc ttacttcttc tattgatgaa actataagat
     8881 gatcaattct tttggtgaac gagtcaacac acgatttcca tcttctgtga tcaacaagtc
     8941 atcctcaata cgtactccac cgattcctgg taagtagatc cctggttcat cagtgatcac
     9001 atttcctggt acaaattgct tttctgcacg agaagaaacg tttggtcctt cgtggatctc
     9061 taaaccgatc ccatgtcctg tagaatgtcc aaatgcttcc ccgtaaccat gttttgaaat
     9121 ataatctcta gcaactgcat ctaattggac accagtcatt cctggttttg cagcatcaat
     9181 aactgctaat tgagcatcta agactatttg ataaatttct tttaattttt ccccaggatc
     9241 accaatagcg aatgtacgtg tcatatcgga aacgtaccct tcgtaatagc aaccaaagtc
     9301 caatgtgatc aagtcacctt gttcgatgat ttttttgctt gctacaccat gcggcatagc
     9361 cgaacgcaat ccgctagcaa cgatcgtatc aaaagaaaca cctgaagcgc caagagaacg
     9421 catataaaag tctagttgat ttgcgacttc aatctcagtc atgcccggtt ggatcatttt
     9481 caggatgtga tcatatgcca tatccgcaat atgacatgct ttttcgataa tagcgatttc
     9541 ttcttcatct ttcacttctc tgagttcttc gatcatctgt gaaacaggta ctaattccgc
     9601 atcgatgatt tcttctaaaa ccgaatattc taaaaatgag actgttgttt cctcaaaagc
     9661 taatgagtgc agttcttctt tgcgtactaa gtttgctacc tcatcaaaaa taggtccgac
     9721 atttttaata atttcaaacc cttgagcctg cgctgccgcc tgctcggtat aacgaaaatc
     9781 agtaataaaa aatgcttttt ccaaagtaat aacagctaat cctgtcgtac ctgtaaagtt
     9841 cgttaagtaa cgaagattgt acggactggt aaccaaaaat gaatccaaat tttcttcttg
     9901 catttttttg cgcaattttt ctacacgtag catcataatg aaactccctc tttctttgca
     9961 cgcttttttc cattatagca aacttttctt cgtacacgac agttaaataa aaatttccgg
    10021 aaaacttctt tttattttaa ataaataaag ccaaaaagga gtcacagtct cgctgcaact
    10081 ccttttagtt tactattttg taaaaactta tgatcgtttt ccaaagaaaa atgagacaac
    10141 tgctaccaaa atcactgcgc ctaaaataga tggaataagt gccatacctg cagcttttgg
    10201 tccccattcg ccgaaaatcg cttgtccaat acttgaaccg actagtccag caatgatatt
    10261 cgttacacag cccatcgaat ttcctttact ggtgatcgca cctgcaattg ctccgatcac
    10321 ggctccaaca atcaatgtcc ataaccaact cataatataa cctccttctt tgctaatcaa
    10381 ttttagcata tgggatttaa aggggacaaa taattggttt tgctatttca tattttcttc
    10441 tgcaaagtaa aaaaataaag aaaacctcct cttagaagtt ttctttattt tttacttgcg
    10501 tttaaacata ctacgaatac cacgtttcac tacatcaaag aaactcaatg attgtaccat
    10561 tctatcttca tctacatatt ctctgatcgc acgaagcact tttttaggtt ctttggaaga
    10621 aaatgtgtat gtaccatttt ttttcgtttg aatggcatat cgaggaatcc attttccctt
    10681 tagcatgact gatgctagta cataatccac ttcttcccat ggaatttgga tatatttgcg
    10741 tctatctcgc gcatggtaaa actcaaaacc attatcacct atcatgattt gcccgtaatc
    10801 tgtcagaccc gtaaacgaag tagcatcaat taccaagtct acttttgtat tgattgattg
    10861 aaccattttc tccctccaga tatttgttta tgaacagtat actatataaa aaaagctcgg
    10921 gacaacgccc gagctatatt tttatggatt ataaaagtcc gatgacgtgt ccaacaacac
    10981 cgactacgaa taaaccaagg atgataacga ttggagaaac ctttttctta agcaaccaca
    11041 tacataacaa tgttaatcct aatgcagcta aaccaggaat caattggtct aagttgtctt
    11101 gtaatgttgt taccttttca ggagaaaggg ctaagccatt attaacttgt tcaaatgcac
    11161 tcttgattcc ttcaccacct gaaggcaatt tatcccattc gatgtaagca cctttatcta
    11221 attttacagt tgatacaact ggtaagaatt tgattgatac ccatctttgt acaagggcag
    11281 ctaatacgaa catccctaaa attgaagcac cttttgttac gtcttgtaac aatccgccag
    11341 aaaggtcatc agtaatttta gaaccagctt tgtagccaaa ttcttgtgta taccacatga
    11401 atgcccaacg aataatgttc caacctaaga agaagatgat tggtccaagg atatttcctc
    11461 ccatagctaa tgaagcacct agtgcaccta gcattggacg tactgtaaac cagaacactg
    11521 ggtcaccaac accagctaaa ggacccatca tacctacttt aaccccttga atcgctacat
    11581 catcaacagg cgcgccattc gcacgttctt cttccagagc caatgtaacc cctaaaattg
    11641 gagaagcaat gtatgggtgt gtattaaaga attccaagtg acgttttaat gctgcagaac
    11701 gttcttcttt tgttttgtat aattttttga ttgctggaat catagaaaat gcccagccac
    11761 cgttttgcat acgttcgtag ttccatgaac cttgaatgaa ggtagagcgc cacgcaacag
    11821 ctaaacgatc tttttttgat aatttgattt tttcttctgc cattttattt tcctccttca
    11881 agattaatag tcattcaaaa tgtcgcctaa agggtcacca gagttgcttc cgcctccatt
    11941 atttgatgaa ccacccattt tagaaaggtt caagtaaata agagctaatg caacacctaa
    12001 agcacctaga gcaatcaatg ttaattgaga aattgcagct acaacgaaac caataacgaa
    12061 gaatggccat acttcttttg tagccatcat gttgattacc aaagcgtaac ctacagctac
    12121 gaccattccc ccaccgatag ccataccatc tgttaaccat gctggcattg attcaaggaa
    12181 tgattgcaca gtttgtgctg ggataaatag caacgctgct gctgggaccg cgatacgaac
    12241 cccttgtaaa catacagcaa tgatgtgcca catttcaacc ttacggatgt ttccttcttc
    12301 agcagccgca tccatcaaat gaacgattgg aacagcgatt gtacgtacga tcattgttaa
    12361 gaacaaccct gcaacagcca aaggaacggc aatcgcgatc gcagaaggaa cacctttaac
    12421 accttgtcca cctaaaacta aaataattgc tgatgctact gatgctaatg cagcatctgg
    12481 tgctacagcg gcaccgatat ttgcccaacc taaagcgatc atctgaagcg taccgccaag
    12541 tacgatacca gcttcgaggt taccggtaac taaaccgatc aatgtacatg ccactagtgg
    12601 ttgatgaaat tgaaattcat caagaatacc ttccatacca gctagaaagg caactaatac
    12661 gactaaaatt attgaaataa tagacatgat aagcctccta taaattttaa tattatttat
    12721 tacgcttttg ctaattcagc ttttgctttt ttcaaaatat catccatgtt atcgcgtgaa
    12781 tcattaggta ctttacgtac atcgaatttc acaccttttt gttcaagctt ctcaaacgct
    12841 tcaacgtctt cttgtcccat agacaatact ttacttacaa cgactttacc gaccgaatgc
    12901 gccattgacc caacatttaa ttctttgatg tctacgccac cttcaacagc tgtcaatacg
    12961 tcttcaggat tttcaaataa aagcaatgct ttggtatttc caaaacgagg gtcttttgct
    13021 acttcgatca ttttgctaat aggaataaca tttgctttta cacctggagg agcagcttgt
    13081 tcgattagtc tcttacgcag atcatccttt gatactgcat ctgaaacaac gataatacgg
    13141 tttggttgca ctgcttttgt ccaagctgtc gcaacttgtc catgtaacag acgtgagtca
    13201 acacgtgcta atacatattt gatttttccg tctccgacca cagttccttc tggaagtgct
    13261 cctttaggtt gtgcgtcttc aactgctgct ttcggtgctt ctgccggttc taattcttcc
    13321 ggttttactt ttacaccgtc ttttgctgtt tcaatgatgt gtgctgcaat ttcatgagca
    13381 gattccattg aaaagcgtga tgcgtaggct tcaatcaaca ttggtaaatt caaaccgctg
    13441 acgattgccc atttatcttt gtgctcttcg aataatgtgt ttgattgatt gaacggtgtt
    13501 ccgccccata aatcaactaa gaataacact tcgtcttgat tatcaaaaga agcaaccgct
    13561 tcttccaatt tggctctcaa gtcatctgga ccttcacttg gtttcaaaat cacggctttg
    13621 acgttttctt gttcgccaaa gatcattgaa ccggattgta agattccttc agcaaattgt
    13681 ccatgacttg ctaggataat ccctaccata tttatacctc ctatttactt ttgttgtaac
    13741 aaattcaaaa atattaataa ttgctaacca ttacaacaga attatttgac cttttccaac
    13801 aaccattctt gctacacctt tttgattttc gctagtagtt ccatcaaaag gattttcctg
    13861 tcagcaggta cttttcgaat ctctaactcc acttcttgac tgttcaagta acgaaaggaa
    13921 tcaatatctt gttgatttac tgaaacaaaa ttggtaatca ttgatttgcc ttcagtaaaa
    13981 ctcataccgc cgatattcaa cgacttcaac tgtacaccgc agcggacaac cgcttcaaca
    14041 tcttggggat tggtgaataa aagcatcact tttgctcgtg ataataatcc actatgatag
    14101 atttgaatca ttttctgcac ggttattaca tttgctgtga tccccggcgg tgcagcttct
    14161 tttagtagaa actttctcaa tttatcttga gcgactacat cgctgatcac aatgatacgt
    14221 tcaacacctg tttgcttcga ccaaactgtt gcgacttgcc cgtggattaa acgatcatct
    14281 atacgtgcca atcgaatatc catctgcaaa actccttctt cctcactact tcacagaaac
    14341 ccctatcatt tctgtgtgaa caaccacctc ctagcacaca ctactataag caaaaagcgt
    14401 gccaactttt ttacgataca ctaattgaat ttaaaggatt cttaaccaat acacaaaaac
    14461 gatacactat aaaagtgtat cgtttttgtg tattcatggc acagtgtatt cttttttatc
    14521 taactgatta tggactagac gtagcagata atacatttct tctatcggca cttttacttt
    14581 tatctgatct tccaacagaa caactgaggc tgccaattgg ctataaagag gatcattcgt
    14641 tgtatttgtc aattcgtttt cttccacagt caatggttca ttccgaataa tacgttcgat
    14701 cattcctgct gtgtgcatca ctaggttgat tttgaatcca tattcttcat cgccgattcc
    14761 cgtcgtctga gagatttgtg tagaaaattg ccacattgga tcgattaact tactaccatt
    14821 aataaagatg aagttatcag agataaactc aacacacgta tttttagctg actcttcatc
    14881 tagggagatg aattcagttt cctccaattc tgattccatc agcaactgat ccaaaatgac
    14941 ttcgatattt tgatcaataa aacgttctaa aggaataaat ggggcttgga tattcaaatc
    15001 agtaatccca gtagtagcta tgatctgata ttctttttgg ataactggca gttcttcttt
    15061 cacttccaca atcgacaaag ctagaacttc taattctgtt tcttgacgct tgtttaccgc
    15121 tcgttcgatc aattctttga ttctttgtgc tgtaccttcg ccagatgcgc agatagccaa
    15181 aacagctttt ttcttatgaa taggttttgc ttcctgaaca gattctacag aagatatttc
    15241 tgcgtaacct cgaaaatttt tcaatgattc atgcaacata tctagatctg tccctaatac
    15301 tgagaccttt cgtacagttt ccaaaactag agaagttgtt accatatcaa cagtacgtac
    15361 tggaattcca gtgtctcttt gaatttgcga attaaacgac gcaagggacc ccatatctac
    15421 taagagtatg accccgcttc cttcatctac ttcttgtaca ttgcgttcaa ttcggtccaa
    15481 tgctgttttc ggatccatat ccaaaggcat gtctactgct cgtacgttct ctgcatctaa
    15541 caactgttgg acaacttgga ccatactgct tgctgtgctg tttccgtgag ctgcaacgac
    15601 tacaccgatt ctgccgcttg cttgatttgc ccgtaacgag acgagtagaa cggtcaagta
    15661 atagtcttca ctttctggaa tctccacttg aaaatacgtt tcgatataac tacgcacttc
    15721 tttcgcaaca gcatattcat ctggaaaatc aaaagccatc tcacggatgt tatcattcgt
    15781 atgccgttct tctcctaagt tgattttttt caaaaaagaa ctgatatgca agctcatagc
    15841 ataaataaag ttttgctgaa actcatatgg taaagatctt ttgaccatta cataaatctg
    15901 attggtcact tctatgattt tcggatctac aaattctgct aatttgctgt ctgcattaaa
    15961 agaaaaacca tggtctttat agaaagactt cagatggatg ttgatatctg tagaaatgaa
    16021 atgattgatt gcttcttgat ccaaaccatc cgacttcagt aaagctgctt tatctccgat
    16081 gatatcatat agattataag gtaactcata ggagtccgat tggattttca tgatcgtatc
    16141 atttggtgct acagttatct ttggctccaa tattttagaa agctctgcca ttgcactgcg
    16201 gttgcttgct aattgaatca atccgctacg aatcccttct gtcaaatcat cgattgtgat
    16261 cgagatttcg ggtgattgca tatgatttag aaatcctcgt gcacatacta actggatatt
    16321 tgacttcaat tgtcctatat ttccataggt cacactgccg atcaatgctt ttgctacatc
    16381 ttctgtcaaa gaaatctttc gctgcgtgcg gtttgcttcg tgggcaatca tgatttttac
    16441 taaatcgact ttctcatttg caggacgttt ttcaaaagcc ggaagtttga tattgattgg
    16501 gatacgccga acaaaagttt ctaatagaga agaacctggg tcttctgtgg tcgcacctac
    16561 gatccgtaca tttgcttcat gggatttcgt tgtttcccca agacgactgt aagttccatg
    16621 atccataaag taaaagatca tttcttgacc ttcaggcggc aaccggtgaa tctcatccaa
    16681 aaacagcata ccgccatccg cttgatcgat aatccccatt ttttcttcat ctgcgcctgt
    16741 aaaagcacct tttgcgtacc caaataaatg actcatcaaa agctcagggt tattggcata
    16801 atcagcacaa ttgaaaacaa tcagttccgt ttcctcatct attacatgat gcgcttttgc
    16861 aaaatgatac atcgcgtgtg caaaataagt ctttcctgat ccggtaggtc ctgtaatcaa
    16921 acaatttaat cctcttggcg gatacaaaat cgcagctttt gcctgttcca ctgaattttt
    16981 catgctgccg ttagctccaa tgatttttgc aaagatatcc ttagtatcta tttggagtgt
    17041 atttttttct tcagagaccg gcgtttcttt gtaactgact acatgttttg taactggctt
    17101 tacgtgcgta acgagtgttt gatatatgta acgtactggt cgtccgtctg tctttatcag
    17161 ttttccttcg cgcactagct gattcagatc tttactgcta tttgttcgct gaatccctaa
    17221 cacttcagca atttcctgtg ttgttactcc ttgatcatat tctgcctttg ttaaatgagc
    17281 ggttttttct ttgacataca tataaattcg gtcaatgcgt tttgccaatg gtatcgctcc
    17341 ttttctatag actattgtac ttaggcttca aaacataata ctatgtattt atttctgttt
    17401 cgtcaactat ttacctgatt ttgttttgtt gataacacgc ggatagatag aaatgttcaa
    17461 tttaaaagaa cgaaaaatcc cgatacgatt tttataatgg aaacaagaaa aagaacactg
    17521 tttaccatat aattatttca ctgatagcaa acaatatagc ttccatcaat tgaaaacgat
    17581 agttatcaaa acatcctgat tccaaattgg caaaaaaata ttaagtgtaa aaagacaaag
    17641 taaacttcgt agcctccact acaactttta tttttataaa caaaaaaggc tgtgacaaaa
    17701 gtcttgtcac aacctcttta atcataagat tcaattaagc ttcttgagct actggataaa
    17761 cagacacttg ttttttgtta cggcctttac gttcgaaacg tactacgcca tcaacttttg
    17821 caaataaagt gtcgtctcca ccgataccca cgtttacacc tggatagatt tttgtaccgc
    17881 gttgacggta aagaattgat ccacctgtaa ctgtttgtcc atcagcacgt ttagcaccta
    17941 gacgttttga ttctgagtca cggccgttag atgtagaacc gccacctttt ttgtgggcaa
    18001 ataattgtag attcatagat aacaacatgg tctgcacctc ctatttatca ttaattgttt
    18061 taatttggat atattctgag ttttcagctt ctatcgattg taatcctaat aaaagatttt
    18121 ctaatagaat ctcagcaata ttgttttgtt cttgtgtcat tcctgaagtg acttcggtat
    18181 aaagataacc gccttcttct tcattcattt caacaatcgg ctcaaaaccg gctaatgagg
    18241 caatcccgtt gacagtgctg atcgctaacg cagaaacacc cgcacaaacg atatcacttc
    18301 cataaggacc agcatcagca tgcccggtca aagtgaatga aacgatctga cctgcgtcat
    18361 ttcgcttaaa tgttcctttg atcatatata ggccttcttt ctcaaacgct attaagcgtt
    18421 gatcgcgtcg ataaccactt ttgtatatgg ttgacgatga ccttgtttac ggtgtgaatg
    18481 ttttttaggt ttgtatttga aagtaacgac tttcttttgt ttgccgtgtt tttcaacagt
    18541 accttcaaca gttgcacctg caacagttgg agcgcctact tttgtagatt ctccgcctac
    18601 taagataact tcgtcaaaaa caactttttc tcctgcttca acgtcaagtt tttcaacata
    18661 gattgcttgt cccacttcaa cttttacttg tttaccacca gttttaataa ttgcgtacat
    18721 gctataaagc acctccttct gatatactta gactcgccat ccgaagtgac agcatatgct
    18781 gtgcttatta cttgttctga gcggttgtag ctgtggagtc cacaattaca acagtactag
    18841 cttaccagaa cagcaaccgt tagtcaacta aatttaaaat caaaaagtaa aacaaaaatt
    18901 caagcaatac ttctgctttt ctatcctcac agttctacca aataaaatag tcgcattgtg
    18961 tttcttttat tcttataata aaagaaaaag gaggagcata aatggaatac ccaaaagaac
    19021 tgatacaaga agcttcttta agaatgaacg gccgtcctgt agaaggattt atcgctgggc
    19081 aaggacctct tcatcctaaa ctgatgctcg tgggagaagc cccagggaaa acagagattg
    19141 atacgcatgt tccttttagc ggtcaagctg gaaaagaatt gatgcaggca ctttcttcaa
    19201 ctggcttaac acgagaagag gtgtatatca ccagtgctgt aaggagccgc ccgtatcgcg
    19261 tgactcatcg gatcaataaa cggacacaac agtcagaaac tgtttatcca aatcggacac
    19321 ctactcgttc cgaagtattt gcgcatgcac caatattgga ttacgaactg atgaaggtcc
    19381 agccaaaatt aattgcgaca cttggaaaca ttggtcttca acgtttactt ggcaaagaat
    19441 atacggttac caaaaatcat ggtcaactct atacagggcc tgtcttacaa ttaacagaac
    19501 aaaaggatgc ctacgaatgg tcagaaaaaa actatcgaat tcttccactt tttcatccag
    19561 cagctatttt ctacaatcgc tcccttgtac ctgttatcca tcaagactgg caagtactag
    19621 cttcactttt acagcaatat taaaaagaac agacaagacc ataaaatctt cagtttacag
    19681 tcttgtctgt tctttttcat tgttttaatg gctaattgca gtatatttcg catgtaaagc
    19741 tgtcaacgca tgagcaactg tttgatgttg atgcgtgaat gtatgttccc acaagtgaga
    19801 aagttgtttt tctacttctg gctgcaagga agtatagata atcgtttgtc ctctttgctc
    19861 tgctttttct tgaaattcat caaaaactgc acgtgccgtc aaatcaatac ttggtacgcc
    19921 tcgcatggag aaaatgacac catcgtgatg atttagttct tccaatttat ttttgagttt
    19981 ttctgaagac ataaaaaaca gtggaccact aatatacact actgtccagt tattcagctt
    20041 ttccgtttct ggcagcccca ttctttgcca atcgatcgtt tctaatgaaa tcgtgatagc
    20101 agcgcttttg acaacaaaaa atacacagcc gccgatcacc ccgatcacga tagcaatact
    20161 taaatcaaag ataactgtac aaatcatcgt aaggataaac aatagaactg ctgaccaata
    20221 tttattagta aatagctcct tgatcgtttc ccattcattc atacgccaag ccgtcacaat
    20281 caaaacacct gcaagtgctg gcattggaat ctgtgacatc actggcgcaa aaacaagcat
    20341 ggagagcagt aagacaactg catgtatcat accagctaat ctagtttgtg caccagattt
    20401 gatggccacg cttgtacggg cgatggccgc agtagcagga atcccgccaa agaaaggaag
    20461 caacagattt ccaattcctt gcgccattaa ttcttgattg ctatctaagg gcttatttgc
    20521 cattcggcca gccgaagcac cacatagcaa gctttcaatc attcctaata aagcaatgct
    20581 aatagctgga actgccactt gttgcaaggc agaaaagcta aaatctccca tattcaaacg
    20641 attactactg atcaatgttt gtggaatctt acccacagtt gctactggta aatggaacaa
    20701 tagcataact gctgttgcca aaatgatagc cagcagcgaa ctaggcattc gtttcgccca
    20761 tttttttgga tagataaaca tccctaaaat gactagccct cccatgatca aagaggctgt
    20821 gtttatcgga aatcctatcg tttgataact acttagtttt tcaataacgt ttgtaccttc
    20881 cgagtgaaca ccaaataaat tatcgatctg acctaatgca ataatgatag caatacctga
    20941 agtaaacccc gtaattacgg gtgctgggat aaaagaagtc agtgttccta atcggaaaag
    21001 tcctgctgca agtaaaaata gacccgctaa aaaagtagca agcaatacac cttgcattcc
    21061 ctttgttgcc gcaatagaca tcaagatagc tgccatcgca cctgtcggac ctgatatttg
    21121 ataaaaaccg cctgataaac cgccaataac gataccggca atgatagccg taatcaaacc
    21181 tgcggccgca tcagctccgc ttgatacccc aaaagccaag gctaaaggca atgctacagc
    21241 agctactgtg atcccagcca aaagatcttt ctgaaatctt gaaagatcat aacctgaaaa
    21301 ttcttttttt agtgaggaaa cataattttt gaacacctat ctaactcctt tttcatgaaa
    21361 ttttctaaaa taccattcat tagaatatca tatttcatga gataaagtaa tatttttcac
    21421 aaagtttcct gttagataaa ttcttatttt ttctatattt ataagaaaat agaaagcaat
    21481 gccaacagag ctagcccacc ttgtttgagt aaaatccaac gatcgctagt caaactgcca
    21541 tataatgcaa caaaaaaaat agatattaat agcatacttg tgatttcttt agatgcggaa
    21601 gaaagataag ctccataaag tagtcccact cctaatagtc cattgtacac tccttggttc
    21661 ttaaacaatg tttgaacaga ttttctgctt aactcttctt tactcatttt aaaaacccta
    21721 cttgtcgtgt cagaatcagt tctcaccgtt tcgatataca tgatataaaa aaattcaatt
    21781 gcaactagag tcactaaaat tctagatagt atcgtcatat cttcttcctc ttttctgatg
    21841 agttttattt ttttgctcct tataattgta ctcgataagg atgttttttt attttatttg
    21901 cctttgaaaa caaacaatat tggcacaaaa aacaaagaaa aaccgttaga aacctcagtt
    21961 tctaacggtt ttttagcgtc acaggaggga ttcgaacccc cgaccgtccg cttagaaggc
    22021 ggatgctcta tccagctgag ctactatgac aagaatcagc ggaacatcaa taattataga
    22081 taattaaatt gcttctgtca atacgaaata gttatttaat tagcaaataa tgaattattt
    22141 tggcccaact actaaatcaa tcttttaaaa cgagacattc tccttcagct gttctaaggt
    22201 tatcaattga aaacaggagc tacattgact tcatgctact cctgtttatt tagatttcct
    22261 gtatccttta attgacttac aaatatagaa acttttacat caagttcgaa ttgagccctc
    22321 gattcaatcg attatctaca ggctgccata atcatgtatc acaactcgat tgtttctgat
    22381 tcctttgtca ttaagatcac accatcacct aatggcaaga ttgtcgatgt caaatcggga
    22441 tgatccatca caacacgaag aaattcattt aatttacgat ggatagctcg tttgccacgg
    22501 ggaatctctt catctgggag caagatcgtt cctccttgga agatgtcatc taccatcaaa
    22561 acgccacctt ttcttaacaa acgtaagcat tcaggtaaaa attcaatata ttttgatttt
    22621 gcactatcca taaaaataaa gtcatacgga ccagacaact ccggcaagat atctgctgct
    22681 tgaccttcaa gcaaagtcac tttatctgtc agtcctagac gttcatagtt ttttttagct
    22741 ttctcgatca tgacatcgaa acgatcaatt gtcgtaacat gagcgttttc tccgattgtc
    22801 gtggccatga cacttgatga aaaaccgata gctgctccga tctccaagat tcgttcaggc
    22861 tgaagctgtc caagtaaaaa tttcaaaaat acaactgttt catggggaat aactggtact
    22921 tctgctacat gagcttcttc ttccagtttg cctagctcac ccggtaattt tttttgctgg
    22981 gtacgcataa aatccaatag ttcttctttg actacaggac gatgcatcat ttcattgcgc
    23041 atactactcc tcctcttcac acattatacg aacaactgac taaatctaca agctgcatga
    23101 gtgttttctc gtgtttcatg ttcagaagag tcgttggatc tctggaatac atttatgttt
    23161 ttaacattct tacaaaaatt tctgtgttcg tctccgcttc tttttcaact ctacaaaggt
    23221 attccatgct gtgattcctg ttaggcaaaa agcaatcgta aagaataacc cctgaatcca
    23281 tgcaaatgcc gtttttgaaa ggactagtcc aatatatgga atctgaaaga tgggactacc
    23341 gataatattt ctctttttta ctactccagg ctcaatataa gtctttgtat cagcttttgt
    23401 ataaaaatct tcgtggttgt tttcaacggc tattacgcga cgcgtcgaga ttttttttcc
    23461 tgaattttca taatatgtaa taatatctcc gactaaaatc tcatttgtat ttttcggtcg
    23521 tacatatatc agatttcctt gtgcataata tggactcatt tctttgtcct caataatata
    23581 aggagtcaaa ccaaaaattc ggggaaccaa gacgacgaga agaataagaa ttaaaaatat
    23641 aatcaacgtt atagaactta taaaagttat ttttttgtaa tcaggtatcg ttttcaactt
    23701 ctttctttat tcaatccttc ttaatcatat aataacgata aatttttttc aaattatagc
    23761 tgtaaaaatg tttcccttta ataattatat gtttctaatt ctatttcctc atctttatct
    23821 cattttttat tataaataat tgacgttagt ttaaaaaacg tttaccctaa cattacaagt
    23881 tttagaaagg aataccttta tgtctttttt taataacata tataataaaa aagaatcttt
    23941 ttatttacgt ataaagtgga gcataatcat tatattatca ttgttatttt taacaggaat
    24001 tgcttatttc ttttatttta ttttttctcc tgcggacagc aatcaaatga aagaaataaa
    24061 agattcgtgg gttttctata caaaagatga tcctgatttt aagtttgaca gcaagtacac
    24121 tcgtacgatt cctaccgtgg ataaagacga aacattcatt atggaaacga cacttgaaaa
    24181 accgttggac gaagcaaatt tactaatcaa aggaaaccat caatggatta ctgtatatct
    24241 aggcgaaaag attttatacc aacggaaaga ttatcaggaa gaaagcaatc ccggactctc
    24301 attggctgtg atagatttac cagcaaatta tatcggaaaa aaattgaccc ttacagtctc
    24361 atctccttat cagaactatg ctggactgcc accgaaagtt tatctaggaa caactggacc
    24421 attgattagt tttatttatt cacaatcagt gccgcaagta ttgacgatga taatcgctgt
    24481 ttttatcgct atagggacct ttttctatac gatctacttg atgtataaac aaaaacgtct
    24541 tgaatactca cttattctct taagttgctt tgctttggca ttaggatttg aagctgtatc
    24601 agaagatatt ttaagtggta ttctttttga accattcgtt cattcttttt tatctcattt
    24661 atttattatc ttaacgtcag ctttcatcat ctgctactat tggagccgaa tgttttacta
    24721 taaaaaatgg tacggaattt tttgtacgtt ccaacttttg atttttatcg gcgttctgct
    24781 ttatgcaact tttactcctg cagaacttcc tgaattaatg ccgattgcta atattgctag
    24841 tgttataagc actttagtca cttcacttgc atgcattggc gaagcatata agaataaccg
    24901 tttttatgta gcttgtacac cttggatcgt cttggtagcc attatccatt gcttgattta
    24961 catccaaacg gcattaggta tctatcaaac aacgatcaat tggtctacga ttttatatat
    25021 tatcattcta atcgttatta tcagctacaa tctaatcgat cacatcttaa ataccgacaa
    25081 agatcggaga caagtagatt tcttaaaaat caaaaatgat ttgttggagc aacattatga
    25141 gcagcttcga cagcatatcc aagaagtaaa tacacttcgc ctggaatttg tccaagagat
    25201 ggagaatctg caagatttaa tgcatacgga ccaaacaaaa gctaaaaact atgtggaaaa
    25261 gatattagaa gaagcaaaaa atttcgaaat gctgtgttct ttttgcaacc accaggtgac
    25321 aaatcttatc ttagcgcgtt accagaagtt agctgccaaa cggaatattc agataacctt
    25381 ccaagcagat cttcccgaag aacttcctat caacgatgac gatctggctc aacttttgat
    25441 ccacgcttta gagcattcct ttagagaaac tcatgccatc gaaaatccac tgtatcgaaa
    25501 aatttatttg tcgatacacg aacaagagga acatttcgtc atttgttgcg aacatagtgc
    25561 acattacgat acaaacctat ttagtcgagg catcacagaa gaattagatg atcaggaacg
    25621 ttttgactta atgatgatga aagacgtagc ggatcgttac acaggaacac taacgcagga
    25681 aaaagatact ttcattgatc ggatcactgt tcaactctct cgaaattacc cgaatcctgt
    25741 ataaaaaata gcggaatcca tatatgtgga ttccgctatt ttttattttc aataaagtaa
    25801 ggtaagcaat tcttctggtt ctatcgcacc aaaatatttc ttcacatcaa tttggctgac
    25861 tatttttgat agagatgctt tatcatattt ctgtccaacc aatttttttt ccacgtcaga
    25921 gatatctcct agtccgaaga aatcgccaaa gatttttgct tctttgatca ttccttctga
    25981 gacattcaaa tggacttcaa tcgaaccgat agaaaaacgt ttttggcgag agaaattgaa
    26041 atctggagac ttcccgtaat tccaatccca atttcgataa tattcctcag agattcgatt
    26101 gatttttttc caatcttcat cagtcaattc gtacgttttt acttcttcta ctgactctac
    26161 gccgaaaatt ttcaacaaga tttcttgacg aaattcttct gttgtcattt tttgcttttc
    26221 ttctggaaga aatggtttga tattcgttac acgagaacgg atcgatttga ttccttttga
    26281 ttcgattttg tcttttttta cttttaatgc attgactact tcattgatat cagagtcgaa
    26341 cataattgtt ccatgagcaa acatccgacc gttggtagca tacatcgcgt tgccagaaaa
    26401 tttcatgcca tcaatgacta gatcatttcg gcctttcaat tccgccccag caacacccaa
    26461 ttcatgaagt gcttggatga ttggctgtgt tactttcgca aagtcacgga atgaatcccc
    26521 atcatcaggc atgataaaac taaagttcag gtttccaaaa tcatggtaga cagctccgcc
    26581 tccacttaag cgacggacaa catgaatgcc attttcttcc acataatctc gattgatttc
    26641 ttcaagcgta ttttggtttc tgccgatgat gattgaaggt tcattgatat aaaagagcaa
    26701 aataggttca tctaaaggca tttcttgcag caaatattgt tcaattgcta aattgattct
    26761 tgggtcgtgg ttttcatttg gtacaaaaat catgttcttc ctcctaaatg ttaaacactt
    26821 tgtcaactgc agcaagttct atatcaatcc ctttgcctgc tgccttttgc gcttcatttt
    26881 tcaattgttc taaatcacca aactgaggta ggtgtgtaag tacaagtttc tttacgttag
    26941 ctgatttcgc aagttctcca gcttcttttg aagtgaaatg ggcatgatga tgttcatttc
    27001 cttcaaaaag ataggtatct gctagaaaca aatcagcatc tttcgcaaaa tcagcgaaac
    27061 tttccaaata acctgaatca cctgtaaata cgaatacttt ccccgttgtt tcttctacga
    27121 tacgcatggc gtaacatact actggatgga ttgtttccat aaaagtaatc agaaaaggac
    27181 caatttttaa ttcttcagct tcaaaataag gatgtccttt tgagacattt tccaacgtca
    27241 attcttcaaa gtgaatggaa tcttttgtgt gtccataaat tgaaagcaat gtctccggat
    27301 ttctcttagg atgaagttgc cagtaatact gtaatacacc aaggtcagca atatgatcat
    27361 gatgataatg gctaaggatc acagcatcta gatcaagtgg atcaaggtgt tcttgtaatt
    27421 tgatcagcgt agcactacct gcatcgatca acaaattgaa cccgtcagat tgaagcaaat
    27481 aacctgttgt cccttgattt ttataaggat acgctcctaa gcatcctaaa acagttaatt
    27541 tcatcgtaca ttccccctca aagatttatc ctaccacaaa agttattttt ttgatgaata
    27601 aactgcttac atgctaagta aaataggcgt ctggacctcc tttttcaatc cgtgcttgtt
    27661 cataaaattc tgcaactgac acattcacat aaacaatgac ccacaaaaga ccgatgaaca
    27721 aagcgattgc tcctagcaaa taccagccga taaaagacag ctgcaacaaa atatacgtac
    27781 caattctatc tttcaataaa aaccagccat atttcaaaca ttgaataacc gacaaatcag
    27841 gatagtcgaa ctttgtccat gcagcaaatt ggaataaacc gccaacaata ctcatgatga
    27901 gtgctgaaac aaaaatcatc cctatcatcg ataaaacaaa gctgatatca agaaaatctt
    27961 ctggcatgac acccgtaggt accgcatttg aactgaaaag cagctgcata taagtactca
    28021 ctccgctgat taaaaacatg ggcagaaaga ttaaactgtt gagtagccag ttgatgactg
    28081 aattgatcaa attgatgcca agtatcgaac caaaatactt tctttggaaa actgaaaata
    28141 aaaggttact ttttggtctg ccaccccgta caactactag cgcaatgtaa aacaatgcat
    28201 agttgaatgc gaataaaagt aaattatcga ctagaaaact caataagtgc tgctgcatgc
    28261 tttcttccgg aaaaggactg aaaccgtcaa aaaagttctg gatgacgctc gtaaatatta
    28321 tagataagaa aacaagccaa gtattgattc cccattgatt ttccaaagaa actcgtgctt
    28381 ttttccgata ttcagaaata ggtattctat tattcattat tcataactcc gaattttttc
    28441 aactgtttca ctatcaagcc gtttcactaa ttctgtgact agtttcactg tattcaaata
    28501 atcatcttca tgaatcactg atgtatggga atgcagataa cgaacaggaa cggtaatggc
    28561 tacagacgga acaccatctc ttgtcaaatg catttgtcct gcatcagttc ctccgccaga
    28621 aatcactgta tattggaatg gaatatccat ttcctctgct acatcaataa caaaattcaa
    28681 taatttttta tgcgggatca tagatgcatc aaaaatcaaa atctgtgggc cttttcctaa
    28741 aatcgaatct gcttcttttg gtgtcattcc tggagtgtct cctgctgtac cagtatctaa
    28801 agcaaaagca atatcgggat ttacaaggtg tgtacttgtt cgagcacctc gcaagcctac
    28861 ttcttcttgc acatcgcttc ctgcgaataa aacatttggg tgtgcttctt tagatagatt
    28921 ctctaatact cgcagagaaa cagctgtccc gatacgatta tcccatgctt ttgccagtaa
    28981 atatttactg ccattcattc gtttgtattc aatatatgga gtgaccatat ctccaggacg
    29041 tatgccccac tctttggctt cttcttggct tgatgcgcca atatcaatga acatatcctt
    29101 tatttcataa ggtttatttc ttacttgagg tgttaaaaca tgtggtggtt tggaaccaat
    29161 cacaccgtgg acgattttcc cttcacgtgt tttgatttca acttgctgag caagcatgac
    29221 ttgcccccac caacctccaa gagtttggaa ttccaagaat cctttttcgg ttatttttgt
    29281 taccataaaa ccaacttcgt ccatatgtcc agaaataaag accttggggc ctccgccagc
    29341 accatgtttg gcaatcacac tgcctaaacc gtcataaaaa atatcatcag cgaatggttt
    29401 ggcatattct ctgaatactt ctcttacctc ttcttcattt ccaggtactc cttttgcaga
    29461 agttaaatca ataagtagct gttcttcttt tgtatccaaa tgttttcccc catttctata
    29521 ttcatattgt gattatacca ttgcttatga tttttgttca ggcaaacttg gtatttttat
    29581 cttttattca gaatattcaa aaataaagaa aacaagcgag caacactcgc ctgttttctt
    29641 tattttaatt gtagtttatc tggtatttga tgttgccaag atttgctaaa aaaagcatcg
    29701 tcaagatgat acttactagc agtaatatga ttttcttcta aaaacggtct gatctgttga
    29761 tctgcagcca accagccttt ccagcctaga tggatcgtgt cttccataaa atagttggta
    29821 cccgcttgat tgacaaaatc agctattcga ttgaatcctt ggctatttaa ttgaaatttg
    29881 atctttttgg caaatccttg taacatttct tgagatagtc cggtataatc gctccatttc
    29941 tcgttaaccg gtgggatgat gaacaatact tcgttatggt tttttgctaa ttgatccaag
    30001 accagctgaa aatcagaaaa ttctggcgaa aaacgataat cccaattttt ttgtgactgt
    30061 ttgagctctg gctcgtgggc tcggatacga tgcgtataaa aatcgttttt caacccgaat
    30121 ggattattcg tcgtttctcg ttctccaatt tgatttgcta aaatggataa ttctgtttct
    30181 tggtattgtt ttggtaatcg ttttgactca tgatcgattt tttgctgtcg atctttcaac
    30241 ccaatattgc tgaatacttc atcctctctc ttcagcatgt tccattggct gcgcagctga
    30301 ttcaaagatt caggtaaagg tagcttccct tttttgatcg tttgcagaat cgccgtcaac
    30361 gtatcattct cttttacttt tgagaaagtt aataatcttc tagctaaata gcgatcagca
    30421 ggcgttactt tcttcattga aaacaaccag tcgtatgttt gaagttcaga ataatacgta
    30481 ttaaagtaat cagtttttac accattttta acgaaccact gaggagaaat aataaagacg
    30541 actttcttgt ttttcatagc atctcctgca gaacgcatca tcatatattg gcttaacgat
    30601 tgtgtcccgg gtgctcccag caaaaatgga cgatagtttc gctggtattt ttctgccaga
    30661 acagacggat gaaatggact gattctgctt aattcacttg agccaaaaaa aggaatatat
    30721 tgtttttcag ataatgcata attttttatc gcatctcctt tcaacacgtt cactgacatg
    30781 gaagaagagg cacgtctaat cgtttctgga ttagagatat ctacttgata ggaggaggta
    30841 aaaaacacaa acagtaagcc aacagctaaa acaaccgggc cgaaaatagc aaaaagtttc
    30901 tttttcatac tcaacccttc aatgcagttg cttgttgaat gattttattc ggtgtgctcc
    30961 aatcctctcg ctcgaattca gatacaggga cttggatatc cagctctccg tctagctcga
    31021 ctaataattg aacagatgct aaagaatcaa gcaacccttc atcaaataga tcaatatcac
    31081 gattttttct tacttcatct gttcctgtta tttcttctaa gattgataaa atttgttctt
    31141 ccattttcct tttctccttt gttatataaa ttaatatgtc actattcgtt agctaaaaaa
    31201 aagtttatct aaaaatcctg aaaaaatcag gaagctaaaa catactgctt gaaatgtcaa
    31261 aaatacggct aagccatgcg taaatttgtt agaaggcaac tgctctttat gcttcttctt
    31321 aaatcggatc catgcatctg tcagacagat cagacaagca tgatacagtc catatacgat
    31381 gtagtaccac gtcaatccat gccatatacc cattagaaga aacaaagcaa aataaccgat
    31441 atttgacatt acgatcttgc ttttaaaaaa ttttttcttc atcatgaaga acatcaatcg
    31501 catatataca taatcacgga accaaaagga cagcgtcata tgccaacgat tccagaattc
    31561 tttgatattc catgataaaa aaggtttatt gaaattgatt ggtgtctgat atcccatcat
    31621 gtagcttgta ccgactgcaa ataagctata gccagcaaaa tcaaaaaaca aatacatact
    31681 gtacacgtac atataaccta ctaatgccca tgaaatacca ccatgctgta aggcaatgcg
    31741 tcccactaca ggaagcagta cttgaccaaa ataataaccg ataatgaatt tatataaaaa
    31801 accgacaaaa atattttgga cgccttttcc caaaagatca acatatttat cacgaacagg
    31861 cggatggagc tgatcttttt caaatctcct atagcgatcg attggtccag aagagattgt
    31921 tggaaagaac agcagaaact ggatataacg aaatggctta tatgttttga tcatgccatc
    31981 tcttatttcc atgatcattt gtacagattt aaaggttaaa taagagattc ctaaaaaacc
    32041 aagtaacgat gcttttccat gttcaaaaaa aggagtcact ttcgttaaaa acagtggaat
    32101 tatcgctaaa ataacggcgg caaaaaacca tcctgtctga tttttccttt gccgataaac
    32161 aaaatagccc catacaagta tcgtctgcca caaaacatac ccaatcagcg ccgctccctg
    32221 ttgccaataa ttacctccaa agctgacaaa taaaaagaaa agtgtcactg ctgattgata
    32281 aactggtaat cttttttccc atatcaataa agatagaacg atcggtacaa gcgcaatggc
    32341 taaaagaaaa aaataaaacg gctctgcata agggatcata tgaggtaaca tcattcagga
    32401 ttcacctcag ctgttaattt ttttcgatcg atttttccat taactgtctg aggcagcaga
    32461 tcgacataat ggaacttctg aggaatcata taatccatta caacttctag taattgttct
    32521 ttgatagatt tagtcaactg aaaatccggc tgtttcgttt tgttttctaa tacgacaaaa
    32581 gcaaccagct gctgtacttt tgttccttgg tattttggta cgacgacagc ttgtttgatt
    32641 cggttgtctt ggagcagata atgttcgata tccccgagtt caatccggta tccatgtaat
    32701 ttgacctgct gatccatacg tccttcataa aataaaagcc catattcatc tagtctacct
    32761 gcatcccctg tatggtaact tgctaagtca ttttgacaaa agaacgcagc agctgtcttt
    32821 tcgggattgt tcaggtaacc ttttgatacg cttggtcctg caataatgat ttcacccgtt
    32881 tctccttttg aaacaggttt accttcttgg acaatagaaa ctttcgtgtc tttttttacg
    32941 tagccaatcg gtagtcggtc ataactatct agtacatgct ggtcaatctg aatacttgaa
    33001 atagctacag ttgcttctgt tggaccgtaa gtattatata tccgtgcatt tgggaatcga
    33061 tcaagtagac tttctgcagt ttttttcgtc aattcttctc cgcaaaataa aaacatgtcc
    33121 aattccggca cattttctcc attgaatgta ggttccatca agcaaatatc cataaatgat
    33181 ggagtagaaa cccacacatt caacttcaag gctggcaatg tcgcgaataa ttgacgaaag
    33241 tcttgaacaa ctgctttatt taacggcttc agcattccgc cagaaactaa tgcaggataa
    33301 atagaaaata ccgataaatc aaaagaaaat ggcgcttggg ctaagaaatg cataccaggc
    33361 ttgatcccaa agtcttccat cgtccattga acaaagctga ctagattatc atgactgatt
    33421 tgtacgcctt ttggttctcc agtagtacca gaagtaaaaa taatataaaa attttctgta
    33481 ccttttacgc tatttctggg ataaaatcct actggtatct tcttgatttc ttgaaattct
    33541 ttttttgtta tgacaggaac cgaaggaata tcttctatcc agtccccgat actgatgacc
    33601 atagatggtt tagccacccg aaagatcgat aggatacgct ccttgggtgt atgctcttca
    33661 atcggcaaat aggcatggcc agatttgaca acccctagaa atgagacaat catttcaaac
    33721 tccaaattgc caaaaacgac aattggtcca tcgccttcta cctttttttc taaataataa
    33781 gcaagtgcgt ctgatgcttg tttcaattca ccaaaagtat gcgtctcatc cgcctgataa
    33841 gcaactcgat caggttcatt tattccccaa tcatcgattg cttcaataat cgttttgatt
    33901 tccatactct tcctcctaaa attcgttata gataaaagtt cctccttgaa tatttttata
    33961 gtgatacaga taaatcagga acagcagtac tgcactataa aaaattgttc gaatgatgaa
    34021 ttttccccaa taattcaatt ttggattaga acgccatttg ttcattggat tccctcctca
    34081 tataacctca cacaaagtgt aactaatttt gacaaaaaga aacatgtgtc tagcttacag
    34141 aaaccgtttt tttactaaca gttttgtcac atagctttta taaaaatagc acatcaaatc
    34201 actgcttttt t
//
LOCUS       DANKHK010000020        33839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000020 DANKHK010000000
VERSION     DANKHK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..1031)
                     /locus_tag="PW109_000866"
     CDS             complement(<1..1031)
                     /locus_tag="PW109_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2510732.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PW109_000867"
     CDS             complement(1113..1802)
                     /locus_tag="PW109_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2510733.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW109_000868"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW109_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2510734.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PW109_000869"
     CDS             complement(2329..2832)
                     /locus_tag="PW109_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2510735.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW109_000870"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW109_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2510736.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW109_000871"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW109_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2510737.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW109_000872"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW109_000872"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2510738.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW109_000873"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW109_000873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2510739.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PW109_000874"
     CDS             complement(6444..7463)
                     /locus_tag="PW109_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2510740.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PW109_000875"
     CDS             complement(7636..8541)
                     /locus_tag="PW109_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2510741.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW109_000876"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW109_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2510742.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDGSWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW109_000877"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW109_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2510743.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PW109_000878"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PW109_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2510744.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="PW109_000879"
     CDS             9754..10398
                     /locus_tag="PW109_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2510745.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="PW109_000880"
     CDS             complement(10454..10957)
                     /locus_tag="PW109_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2510746.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="PW109_000881"
     CDS             11554..12519
                     /locus_tag="PW109_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2510747.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="PW109_000882"
     CDS             complement(12618..13790)
                     /locus_tag="PW109_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510748.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="PW109_000883"
     CDS             complement(13777..14922)
                     /locus_tag="PW109_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510749.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="PW109_000884"
     CDS             complement(14936..15868)
                     /locus_tag="PW109_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510750.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="PW109_000885"
     CDS             15985..17064
                     /locus_tag="PW109_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2510751.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="PW109_000886"
     CDS             17061..17690
                     /locus_tag="PW109_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2510752.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="PW109_000887"
     CDS             complement(17800..18027)
                     /locus_tag="PW109_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2510753.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="PW109_000888"
     CDS             complement(18130..20718)
                     /locus_tag="PW109_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2510754.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="PW109_000889"
     CDS             complement(20738..21397)
                     /locus_tag="PW109_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2510755.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="PW109_000890"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="PW109_000890"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2510756.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="PW109_000891"
     CDS             complement(22170..23036)
                     /locus_tag="PW109_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2510757.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="PW109_000892"
     CDS             23145..23786
                     /locus_tag="PW109_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2510758.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="PW109_000893"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="PW109_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2510759.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="PW109_000894"
     CDS             complement(25227..26126)
                     /locus_tag="PW109_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2510760.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="PW109_000895"
     CDS             complement(26128..26925)
                     /locus_tag="PW109_000895"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2510761.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="PW109_000896"
     CDS             complement(26903..27607)
                     /locus_tag="PW109_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2510762.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="PW109_000897"
     CDS             27738..28313
                     /locus_tag="PW109_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2510763.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="PW109_000898"
     CDS             28431..29108
                     /locus_tag="PW109_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2510764.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(29187..30995)
                     /gene="pepF"
                     /locus_tag="PW109_000899"
     CDS             complement(29187..30995)
                     /gene="pepF"
                     /locus_tag="PW109_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2510765.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(31171..32169)
                     /locus_tag="PW109_000900"
     CDS             complement(31171..32169)
                     /locus_tag="PW109_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2510766.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(32395..33048)
                     /locus_tag="PW109_000901"
     CDS             complement(32395..33048)
                     /locus_tag="PW109_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2510767.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(33380..33778)
                     /gene="spxA"
                     /locus_tag="PW109_000902"
     CDS             complement(33380..33778)
                     /gene="spxA"
                     /locus_tag="PW109_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2510768.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         9951 a         7164 c         5973 g        10751 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaaccgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa aaatacgtcg actcccttac aagagagccg acgtattttt ttcgtttcta
    29161 ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga tcaattcttc taattcattt
    29221 agacgttgtt caaacatcga cattgcatct tcaatatatg cagcctgtgt catatcaact
    29281 cctgcttttt tcattacttc cacaggataa tcgctgttac ctgcttttag gtaagccaag
    29341 tagttctcta gtgcctctgg ttcctggttt agtattttct tcgccagtgc tgaagcagca
    29401 gaaaagccag tcgagtattg gaaaacatag taattataat aaaaatgcgg tatccgtgac
    29461 cattcgaatt taatttctgg gtcttcttcg actgctggac cataatattt tgcattcaat
    29521 ttaccataac tatcacttag gtattcacta gtcaatggca cacctttttc atcttccgta
    29581 tgcatgaaat gctcgaattc agcgaattgt gtctggcgga aaactgttcc tttaaaccca
    29641 tccaagtaat ggttgagtac ataagcccgt acacgagggt ctttttctgt ttccaataaa
    29701 tactccgtta ggatattttc attcgttgtc gaagcaattt ctgctaaaaa gatggagtag
    29761 tcgccgtaca cataaggctg gttcgaacga gtgaaataac tatgaacact atgtcccatt
    29821 tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc aattcaataa aatatatgga
    29881 tttgtgtcat agcttcccga agaataagca ccgcttcgtt tccctttatt ttcgacaaca
    29941 tcgatccaac gttcagagaa tgctttttct acgatggcca tgtattcttc acccattggt
    30001 ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg taaaggtaat tggagcttcc
    30061 cctaagaccg gtgtataaag atcatacatg tgcagttttt ccacttctaa taaacgtttc
    30121 cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt ttaccacgtc taccaaagta
    30181 tcgtatacac tttcaggaat atgattattg ctcaaagacg cttctctggc agagctgtaa
    30241 ttacggactt tcgctttaaa attatgtcct tttatatgtg tgcctaaagt agaagcaaat
    30301 gtatttctaa attgttcgta aacactgtac aatcccttaa atgctgcttc gcgcaccctt
    30361 cgatccgtgc tttctagcaa ctgaccatac acgccatgag ataattggac tatttcacca
    30421 ttttctcctt caatcgttgg aaaaactaga tctgcattat tcaaaacagc aaatgtatca
    30481 cttgaagcat caaagatttc acctgctcca gcaagaagag attcctgctc agcagataag
    30541 acatgggctc gattatctac tatttgctgg atataatggc gatagacttc caattttggt
    30601 tcttctttga aatattgcca aatctggtca tctgacaatt gcaatatttc tggttcaaac
    30661 caggaaacgg cttcactaac tttcgaaaac agactgcttg ctcttgcata aagcgcttgg
    30721 taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg catatacata aatgacttca
    30781 gtttggcgat aaaccctcaa tacgaattca atcgcattta aaaattgaga agcgccttga
    30841 tcaagtgttc ctttgtgttt ttcagattgt tttaactctt ctgataattc caaatatttc
    30901 tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt cccaagttaa attttcaggc
    30961 aattcttctc gttttggcaa ctgctttact tccatgaaat ccactccttt tcttccctta
    31021 gctctatcta tcaaaataga tcatagtcta acatccacat gtcacagcat ctaacttttc
    31081 tactcattca tgtgtctgct tccatcaatt ttagcataac taaatcttgt tttacataga
    31141 aaaccgattc ctagcctatt tttatactta ctattttttc aaagaagaca agtgtatgca
    31201 ttcgttaaaa aactgctgat agaccatctc ttcatcaatc ataggaaaaa gccattccct
    31261 tttatgttct tttatttggc aaagccatga gttgaaatca ggaactttct ggttttccaa
    31321 ggcttcttcg tacaacattc gataaagaaa aacctgttct tgtaaataaa agaaaaaccg
    31381 acttttttga tagatccacg atgatagatg caaaagatgt ttatgacgca agtagcattg
    31441 ttcttgtatg gatactgtat tcttttgctt gtgaataagt tggcgtgtca gccatttctt
    31501 cccatcttca ctcacttttt ctattttttt catttggttc attttaattt cgttactagt
    31561 gaatatttct ttaaggtccc tactagaaaa agtccagcag ctactgcaaa aggaaatatg
    31621 ccctgataca gtctctgtaa tatgatgata caaataaagc ttttgtttct tgagatcagc
    31681 tttccagcaa tgcactcctc tttttctatt atagtagcag caactttttt ctatcaatga
    31741 gaattgcgat tgtccaagaa aagaattacc aagtatccac catacttggt aactgttatt
    31801 caaataattt tgggttcttt caataaatct ctggtgagac agtctagaac attggatttc
    31861 tattgctaga ttcgccaaca aaatatccgg cctttgccgc aacctcggca aataggcttc
    31921 caaaaatact ggttctactg actgtcccag ccaatccatg aagcattcct ttaactgtaa
    31981 gtgctctttt gattctcttt cactaaagac cgaacagtta tctgattttt gatgggcaaa
    32041 atgagcacat tttttctttc cctttttcaa aatcactctt tttcgacagc cacgacaata
    32101 atatttcttt cctttttctg catgtttagc aaaacagcac ttattatatt cattcattgc
    32161 aagcagcatt tttctccctc tgaacttatt tacgtctaag aatttattta cacgaaaaaa
    32221 gagggcgtga aaaagctatt ctgcatcaag taataagaac agctaaacaa aaatagttcc
    32281 caatattttt cgtttggttg ggcttgttac cgcagatgca agcttttgaa cacatttata
    32341 cgaaaaaaag actgcgacac aatttttgtc acagtctctt actataatgc tgatttatac
    32401 aaaataaaat ttcgttcgct cgatagcatt acgcgccatc agacattgtc catattcatt
    32461 caatgtttct ggtgtacgat cgctcttttc agcaaactcc aaaattcgag caatctgatt
    32521 ctctacatcc gcttggttga gattttccat ccagaagtga acgactaggt aatagcgctc
    32581 ttcgaataaa tacaaatcag tccaagcaga ctctagttcg acatctgttg caagttggat
    32641 cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt tcctgttctt ctagggcgct
    32701 tccagataat tcttcctccg gatcgctgcc aataatctgc tgacgaagaa agtcactcac
    32761 atcttctgaa tcaacagtag aatcttctga ttgagaaatt tcttcatccg gaagattttt
    32821 actgataaac agttctaatc cgtctccttt tggcaaaact tggaaagtga cagcttcgct
    32881 tcctcggaac tcctcttcga tatcaacttc ttccaaaata ctataaaaga aattttcgat
    32941 ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc cgttcctcta aatcttcatg
    33001 accaattagt acacggatcg tattttcatt aatatgttcc atttccattt tgctacacct
    33061 cactttgcca caatgtttaa tatagattca ttgtagcgaa atcagcttaa atgtaaagaa
    33121 aaaaaatctc gctgttaagc gaatttaata aaaccagcgg atttttcaac ctttcaagct
    33181 atcttattta actataacat ttttggcaga cgaatactat atagccgact tttcagctaa
    33241 ttttccctct ctatttttcg tgcaatcatc atcttctact acctttttat acatctaatc
    33301 aagaatactt tttgtaaaaa caaaaaaact tggaaaaact atctttgtaa tagtcttttc
    33361 caagtttcgt attatccttc tataatccag ccatcaattg tgcttgacgt aattctaatt
    33421 gtcgtacatc gcgcggtagg aaccgacgaa tttcatcttc attaaaacca acttgcagac
    33481 gtttatcatc aatcatgatt ggacggcgaa gcaatccagg attgttttgc acaagttcca
    33541 ataattcttg taacggcaga tcatctaaat ccatgttcag tttttggaat actttggaac
    33601 gtgtcgagat gatttcttct gttccgtctt ctgtcatttg taaaatcgct tttaattcag
    33661 aactgttcaa tggttctgaa aaaatattac gttcaacaaa tggaatctga tgttcttgca
    33721 accacgcacg agcctttctg caggaagtac aacttgggga agtatataat gttaacaacg
    33781 tgcaccactc cttttcgtga taagaatcac ttttttgtaa cagaagatca taataatct
//
LOCUS       DANKHK010000021        32821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000021 DANKHK010000000
VERSION     DANKHK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(92..217)
                     /locus_tag="PW109_000903"
     CDS             complement(92..217)
                     /locus_tag="PW109_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510769.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(351..1244)
                     /locus_tag="PW109_000904"
     CDS             complement(351..1244)
                     /locus_tag="PW109_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2510770.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1426..2352)
                     /locus_tag="PW109_000905"
     CDS             complement(1426..2352)
                     /locus_tag="PW109_000905"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2510771.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2437..3198)
                     /gene="pflA"
                     /locus_tag="PW109_000906"
     CDS             complement(2437..3198)
                     /gene="pflA"
                     /locus_tag="PW109_000906"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2510772.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3442..5673)
                     /gene="pflB"
                     /locus_tag="PW109_000907"
     CDS             complement(3442..5673)
                     /gene="pflB"
                     /locus_tag="PW109_000907"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2510773.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(5950..8400)
                     /gene="parC"
                     /locus_tag="PW109_000908"
     CDS             complement(5950..8400)
                     /gene="parC"
                     /locus_tag="PW109_000908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2510774.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8412..10457)
                     /gene="parE"
                     /locus_tag="PW109_000909"
     CDS             complement(8412..10457)
                     /gene="parE"
                     /locus_tag="PW109_000909"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2510775.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10623..11057)
                     /locus_tag="PW109_000910"
     CDS             complement(10623..11057)
                     /locus_tag="PW109_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2510776.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11184..11822
                     /gene="plsY"
                     /locus_tag="PW109_000911"
     CDS             11184..11822
                     /gene="plsY"
                     /locus_tag="PW109_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2510777.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(11954..12829)
                     /locus_tag="PW109_000912"
     CDS             complement(11954..12829)
                     /locus_tag="PW109_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2510778.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(12911..13702)
                     /gene="codY"
                     /locus_tag="PW109_000913"
     CDS             complement(12911..13702)
                     /gene="codY"
                     /locus_tag="PW109_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2510779.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13720..15120)
                     /gene="hslU"
                     /locus_tag="PW109_000914"
     CDS             complement(13720..15120)
                     /gene="hslU"
                     /locus_tag="PW109_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2510780.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15134..15682)
                     /gene="hslV"
                     /locus_tag="PW109_000915"
     CDS             complement(15134..15682)
                     /gene="hslV"
                     /locus_tag="PW109_000915"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2510781.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15719..16624)
                     /gene="xerC"
                     /locus_tag="PW109_000916"
     CDS             complement(15719..16624)
                     /gene="xerC"
                     /locus_tag="PW109_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2510782.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16822..18198)
                     /gene="trmFO"
                     /locus_tag="PW109_000917"
     CDS             complement(16822..18198)
                     /gene="trmFO"
                     /locus_tag="PW109_000917"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2510783.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18167..20245)
                     /gene="topA"
                     /locus_tag="PW109_000918"
     CDS             complement(18167..20245)
                     /gene="topA"
                     /locus_tag="PW109_000918"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2510784.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20367..21224)
                     /gene="dprA"
                     /locus_tag="PW109_000919"
     CDS             complement(20367..21224)
                     /gene="dprA"
                     /locus_tag="PW109_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2510785.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21280..22047)
                     /locus_tag="PW109_000920"
     CDS             complement(21280..22047)
                     /locus_tag="PW109_000920"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2510786.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22040..22900)
                     /gene="ylqF"
                     /locus_tag="PW109_000921"
     CDS             complement(22040..22900)
                     /gene="ylqF"
                     /locus_tag="PW109_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2510787.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23160..23558
                     /locus_tag="PW109_000922"
     CDS             23160..23558
                     /locus_tag="PW109_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2510788.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            23837..24337
                     /locus_tag="PW109_000923"
     CDS             23837..24337
                     /locus_tag="PW109_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2510789.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(24420..24971)
                     /gene="lepB"
                     /locus_tag="PW109_000924"
     CDS             complement(24420..24971)
                     /gene="lepB"
                     /locus_tag="PW109_000924"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2510790.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIL"
     gene            complement(25123..25350)
                     /locus_tag="PW109_000925"
     CDS             complement(25123..25350)
                     /locus_tag="PW109_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2510791.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25347..25865)
                     /gene="msrA"
                     /locus_tag="PW109_000926"
     CDS             complement(25347..25865)
                     /gene="msrA"
                     /locus_tag="PW109_000926"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2510792.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(25885..26598)
                     /locus_tag="PW109_000927"
     CDS             complement(25885..26598)
                     /locus_tag="PW109_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2510793.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26601..27497)
                     /locus_tag="PW109_000928"
     CDS             complement(26601..27497)
                     /locus_tag="PW109_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2510794.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27655..28497)
                     /locus_tag="PW109_000929"
     CDS             complement(27655..28497)
                     /locus_tag="PW109_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2510795.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28629..29282
                     /locus_tag="PW109_000930"
     CDS             28629..29282
                     /locus_tag="PW109_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2510796.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29345..29872)
                     /locus_tag="PW109_000931"
     CDS             complement(29345..29872)
                     /locus_tag="PW109_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2510797.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29892..30839)
                     /locus_tag="PW109_000932"
     CDS             complement(29892..30839)
                     /locus_tag="PW109_000932"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2510798.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(30851..32737)
                     /locus_tag="PW109_000933"
     CDS             complement(30851..32737)
                     /locus_tag="PW109_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2510799.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         8652 a         6909 c         5683 g        11577 t
ORIGIN      
        1 aatctaaaag aataacccaa gattggataa attattaatg actcgccatt cgtttcttag
       61 tgattctgga ttatcaaatg gacttcctaa tctaatcagc tagttcttcc cataaaatgc
      121 catacttgac tgaatcataa tattgattat ttaaaaatct aacttttctt atcactactt
      181 cctttgtcat tccacatttc tcaccatttt tttgcattcc tttgtttcca gaccatgtag
      241 tgaatctaat aggtggtaga taggcaaaat actcaaataa ttcttttaac cataaaggaa
      301 gagaaaggct cctcctgcaa gagtcagtat aatttttgct tactgctctc ttaggtttta
      361 ctcactaaaa ctgttccata ttctttgata taagaaaatg catgcggtat aaatttcttg
      421 ctcagctcag aaaagatttg tcgtttaaga aaggccattt cctccattgt caatgaagga
      481 tccaatgttg gtaattcaat ctcaaaattg tttttttcga caataggtaa ttctaatgtg
      541 aatgattctt cttcctttat agtattcgtc acaagtagag actgatggaa aatactaggc
      601 cggcgatttt tatatttatt cttacgtatc ctgtcaacga taacttctat ttgatcaaaa
      661 aagtacgatt catttacgtc tccaataagt tcggctaatt ctttgaatgc cttcgttaca
      721 ggaagctgat tcttctgtac aaaaaagtaa ttagccaaag taagcgggaa gtgttctata
      781 gaggaaagtg tgataaaacg gtacttctca tttcctgcag ttgtcctcag caattctgtc
      841 tctcggctgt ttcttactcc ataaaaatac gcttctttcc gttcatcttc gaaacaatac
      901 gcccaaataa cttctcccat cgctaatttt tgttcatcaa cagagagtct cttcaattgt
      961 tcagctatgg tttcagcagc cgaagtggct tgttgcaagt aatctttagg atcaaatatt
     1021 ggaaagttca aatgatatct tcgttcttcc ctcttgatta atcctgcatc gatcaataaa
     1081 tcaagaaaat gggaaaattt attttgcgaa aattcttttt tcaattctct tagaatcgtt
     1141 tcttgttctc gatgttcata tagataatcg gtcaatgaag aaaaaagcgg atcatcggtc
     1201 agtttttgat aaaagttttc cttatttttt gaataatagt tcatatatgt tctcttttct
     1261 atccgtttta ttttacttaa aaggctttta cgtaaaaagg aggaagaaca agagtcataa
     1321 ttgactgtta ttctcctcca tttcttcggc ctttagtatg ttctatgttt ctttttatat
     1381 caatcgctta tccattcatt ttgaatgctt catactacca tttgattagc taaatacagc
     1441 cgtcaattga ggtacgactt gttttttacg tgaaacgaca cctttaagca aagcacggtt
     1501 gttggtcaaa gttgtttgga aagcttcttc tactttttct tttcctttac cagcaacaag
     1561 taattctgaa tcactgtcta agatgtttgt gacaactaat acgaacagat cgtagccatt
     1621 tgctttcatt tcatcttcca ttgctgcttc tagctctgct tgtcgatcca tcacttcatt
     1681 aaggtccact gtattgactt gagcgatacg aacgttggaa ctgcccattg ggaaactttt
     1741 tgcatccaaa tcaagaagaa ccgcagctga tttatcactt agatttgttc ctgctttcaa
     1801 catttcaagt ccataagtgt ttagatcgac ttcagcgatt gctgcaagtt cttttgcagc
     1861 tgcttcatct tcaggcgtac atgttggtga tttgaacaac aatgtatctg aaatgatagc
     1921 tgagagcatg attcctgcga tttctttagg aattgttact tggttttctt tatatagttt
     1981 taaaataatc gtacttgtac atccaactgg ttctgcacga tagtacaatg gattagctgt
     2041 ttcaaagttt gcaatacgat ggtggtctac tacagctaaa atatttactt cgctgatatc
     2101 tgaaatactt tgttggaatt cattatgatc cacaagcatg acatctgcat tttctgcttt
     2161 agctgactct actactcgtg gtgcagatag ctgaaagtgg tctaatgcgt attgtgtttc
     2221 ttcattaggt gtacctagag caactgcttc tgtttcttta cctaattcat tttgcaagtg
     2281 tgaaaacgca atggctgccc cgattgcatc tgtatctgga ttttggtgac caaaaactaa
     2341 aatttttgac atgtcattcg ctcctttgtg atttctccta tttaataata gcaaaaaaac
     2401 ggaaagaagc aatacttatc ttctttccga tgcgacctat cgcgttaaat attttgtata
     2461 ctcttctgta tgaagcaatc gtttagcatt ttcgacacgt tcttttgtag gtggttcgat
     2521 cccttctaat ggatatttca aaccaagtgt ttcccacttg tattttccca tcgtatgata
     2581 aggaagaatc tctactctgt ctacatttga caaggtttga ataaagttgt ctagtcggat
     2641 caaatattca tcataatcac ttcgctcagg aactagtaca tggcgaatcc aaaccggttt
     2701 gttgattttt gacaaatatt gagccatttc taaaatattt tcattgtccc aatgcgttag
     2761 ttttttatgc tcttcattat cgatgtgttt gatgtcaaat aacagtaaat ctgtatactt
     2821 catcaattct tgaaaacgtg aaaagaaagg ttcttcatac gtaaaaggtt ttccgcatgt
     2881 atccaatgta gtatgcatct tccgctcttt tgctttttta aatagttcga tcaaaaaatc
     2941 gatctgcaat agaggttctc caccactaac agtaatacca cctttatctc cccagtaagg
     3001 tctgaagcgt tctgctttat ctaaaagttc atctgctgtg tattctttcc ctccgccaat
     3061 attccaagta tctggattgt gacaaaattg gcaacgcatg cggcatcctt gcataaacac
     3121 gacaaagcgc agtccaggtc cgtctactga accgaaagtt tcgatagaat gaacatagcc
     3181 gattgttttt tcttccattt aagttcacat cttttctttg atttcatttt aagtacaagt
     3241 tagctgtcga agcgaagctt tctgaacaaa atttttcgca attgccgctt cgttatgtgt
     3301 aaaatttaca gggacggaac atacaagtcc tgcccctgta aatcgtttct ttcaaataag
     3361 catactgatt attcgatttt gatgcgatgg ctattcaacg tcctttcgac gctaagtcga
     3421 aaaacttttt tcacgaactt tttacattct gtcgtgtgaa gttctcatca agacatctaa
     3481 ttgttgttct cttgtcaagt cacggaattt aactgcataa ccagataccc ggatcgtcaa
     3541 gttcggatat ttcattggat tttccattgc atcacgcaat aaatcattac tgaatacatt
     3601 gacattcaaa tgataagctc cttttgagaa gtatccatct aagacattac gcaagttttc
     3661 gattcttgta tcttgttctt ttccaagacc tgaaggattg attgtttggg tatttgaaat
     3721 tccatccaat gcacagccgt aattcatttt agctgttgag ttcaggctag ctaataaccc
     3781 actttgttcc gcattgtaac ttggatttgc tcctggcgca agtggttttc cagcttcacg
     3841 gccatcagga gtatttcctg tatttttacc gtaaacaaca tttgaagtaa tcgtcaataa
     3901 tgaagttgtt ggttttgagt tgcggtaagt tttatgacgt ttgatttgtc ccatgaagta
     3961 ttccaagatc catgaaacta attcatctgc acggtcatca tcatttccgt atttagggaa
     4021 gtcgccatcc acttcaaaat caactgctat tcctttttca ttacggattg cttttacttt
     4081 tgcatatttg atcgctgaca agctgtctgc cgcatgagag atcccagcaa tacctgtagc
     4141 aaatgtgcgt tgcaagtcag aatccatcaa agccagctga gctgcttcat aagaatattt
     4201 atcatgcata taatgaatga tgttcaacgt attgacatat agttctgcta accaatccat
     4261 catattcatg aagttatgtt tcacttcttc aaagtctagt atttctgatg tgattggacg
     4321 atattctggt ccaacttggg cgccagtcac ttcatcgact ccgccattga tggcatataa
     4381 tagtgtttta gccaagttag cacgagcgcc aaagaattgc atgtcttttc cgacaactgt
     4441 tcctgataca cagcaagcaa tcgcacaatc atcagaaccc cattgttcac gaagctgatt
     4501 atcattttcg aattgaatcg aagaactttc gatagcgatt ctgctagcat atgttctgaa
     4561 tccttctgga aggtgttcag aatacaaaac agtcaagttt ggttctggtg aaggtcccat
     4621 atttgttaat gtatgaagca gacggaaatc attctttgta actaatgaac gaccatctaa
     4681 tcccatacct gcaatggata aagtagccca gatagggaat cctgagaata attggttata
     4741 ttcaggtgta cgcgcgaatt tgaccatacg taatttcatt actaagtggt cgatcaattc
     4801 ttgtgcttct ttttctgtga ttactccatt atccaaatca cgttggatat agatatcaag
     4861 gaatgctgag atacgtccga ttgacatcgc agcaccattt tgtgatttga ttgctgctaa
     4921 atatccgaag tatagccatt ggacagcttc ttttgcattt gcagctggtt tagaaatatc
     4981 atagccatat ttcgcagcca tttctttcat ttgcgctaat gcacgatatt gttcagtgat
     5041 ttcttcacgt aaacgaatca cttcttcact cattgtatga taaccagtca tatcatgatc
     5101 ttttttcttt tgttccatca aatagtcgat tccatacaat gccacacgac gatagtcacc
     5161 gatgatacgt ccgcgaccgt aagcgtctgg aagccctgta atgattttgt ttttacgagc
     5221 agcacgcatt tcagcagtat aagcgtcaaa tacaccttga ttatgtgttt tgcgccagtc
     5281 agtgaaaata tgtgtcaatt catcgtttgg tgtatagcca ttgcttttta gcgcattgtt
     5341 agccatattg attccgccga acggcatgaa tgcttgtttc aaaggtttgt ctgtctgtag
     5401 accgacgatt ttttccaaat ctttatccaa atagcctgca tcgtgtgaag taatagttga
     5461 tacgatttct gtgtccatat catagacacc gttacgctcg aattggatat catttaactt
     5521 ttgcaattca tcccataatt tttctgttgc ttctgttggg ccttccaaaa agctgtcatc
     5581 accgcgatat tcagtgtagt tattttgaat aaagtctcta gtgtctacgc cttctttcca
     5641 tttggtacct ttaaatccat tccattgctc cattgtatgg acctcctaaa tttctgtttt
     5701 atgtaacagt ttttacgtga ttagtataac acagcaccct atctttgcaa gggttttctt
     5761 gttttttatt taacaatttt tatgttttat ttacatatat ttaaatcaac gttgacatac
     5821 caacatttaa gcattgtgaa cactttcatc tttttttgca aacgtataag gatttattaa
     5881 aaatgaatga aaaagagacg ccacctgtat taatacagat ggcgtctctt cattaaaata
     5941 ctacaacagt tacgactcga tgaaaatctc aggagcatcg ataacttcga tgacttctcc
     6001 accttttttc tcatccatcg caaatgatcc attcgatgtt cgatcaccta tagggaaatc
     6061 agttgggtca atcgttaact gctgcccttt ttgattcatc aaggttattt cttttgatct
     6121 tccttccgac atatagataa tacggtgcgg attttttttc agctcacgca tgaccgtcac
     6181 tccccgcttc gcacgtgaag tttggctgat ttcttgagca agcatgcgtt taacagctcc
     6241 tcgctgagta acgattacga tcggcgtatc accttctgct aacacaagca gcccgttgat
     6301 aatcctatct ccatctttca aattcataga cttcactcca gctgcctttg ctccaacaac
     6361 gggaacatct gttaaaggat agcgcaaacc cattccttga tgcgttacta agaaaacatc
     6421 taagcctggc tgatcttctt tcagatacac agcaatcagt tcatcttctt ccgcctttag
     6481 cttcatacag ctgagcggac ggctcttata ggtgcgccat ggttcgaact ctgtcattcg
     6541 tgtttgcttg atcaatccat tcttgctgat gaatacaaat gttttctctg cttgaatttt
     6601 ttgatatggg aaaacagcta aaatcgattc gtctacagac agattggaga ttgtttgaga
     6661 aatgtgctca ccagcatctt tccatttcaa atcgggtagt tcatggacag ggcggtagat
     6721 cacatttgct ttatttgtga tcagaagaac atggtccaat gtactcaatt cacctgagta
     6781 tagcaagtag tcgccttctc gcatcccgat ttcttctggt ttggaagcac tataagaacg
     6841 aaggcttgtt cgcttcacat agccttctcg cgtcacactg acaattacgt cttcttgtgc
     6901 cactaagact tgcgtatcaa ttttgatttc ttcgatttcg tcttcgattg tcgttagacg
     6961 agcggaagaa taattttttt tcacttctcg caattctttt ttcataacag agaaaagctc
     7021 tttgtcattt gaaaggattt tattcaactc tgtgatttgc gcaatcaagc tttcggattc
     7081 cttttgaagc tctgtgatat ccgtgttcgt caaacggtat aattgcaatg tgacaattgc
     7141 ttctgcttgt tcttctgtaa attgaaagac gtcgactaaa ttctttttgg cgtctttttt
     7201 gtctttactt tcacgaatcg ttgcgattac ttcatccaga atcgataaag ctttcatcaa
     7261 tccttctacg atatgctgtc ttttttgagc tttggctaaa tcgaattgac tacgttttgt
     7321 tatgacttgc tttcgatgct cgatataact tcttaaaatg tctttcagtc caacttgatg
     7381 cggagtcata tggtcgatag cgaccatatt gaaattgtaa ttgatctgta gctcagtgtt
     7441 tttgaacaaa taattcaaaa ttccttgagc attggcgtct tttttcaatt cgacgacaat
     7501 ttgcagacct gttctatcac tttcatctcg tacttcagct attccatcga ttttcttatt
     7561 caaacggatc tcatccattt ttttcaccaa ggtcgcttta tttacttcat atgggatttc
     7621 tgtgatcacg atttgctgtt tattaccctt cattgcctct atctttgttt tcgaacgaag
     7681 aataactttt cctcgtcctg tttcataagc ttttttgatt tcatcttttc cttgcaagat
     7741 ccctcctgtt ggaaagtcag gtccaggaat atattccatc aatttttcaa ggctcgcttg
     7801 tggatgatcg atcatataga ttgtgccatc gatcacttca gctaagttat gagtcgggat
     7861 ttctgttgca tatccagcag atatacctgt cgatccattg acgagaaggt ttgggtatcg
     7921 agctggaagg actgttggtt ctttttctgt atcatcaaaa ttccacacga gatcgactgt
     7981 ttctttctcg atatctgcta gcatctctcc acttaacttg gataatctgg cttcagtata
     8041 acgcatcgca gcaggtggat caccgtccat actcccgtta ttcccgtgca tttcgatcag
     8101 tacttcccgc aacttccagt cttggctcat gcggaccatc gcttcataaa tactgatgtc
     8161 accatgagga tgataattcc ccatgatatt tccgacagat tttgctgatt tacggaatcc
     8221 tttgtcatac gtattgccat ctttgttcat ggcaaataat atccggcgtt gcactggctt
     8281 caatccatca cggatatcag gcagtgcacg ttcttggata atatatttgg aataacgtcc
     8341 gaagcggtca cccatcactt cttctaaagt taattcttgt acttcttgtc gattttccat
     8401 caggtgacct cctattctac ttcaaataac tgattgtttt catttttatc tgctcgttct
     8461 tcatctaata aatcattaga aactgaagga gagatttcag tatcctcttt tttatctaag
     8521 atactgccat cttcttcgag cgtaaattgc acgtgatttt cgatccattt tcttcgaggt
     8581 tctactttgt cacccatcag tgtggttact cgacgttcag cttgcgcagc atcatcgata
     8641 cggacgcgga tcaacgtacg ggaagtcgga tccatcgttg tttcccacaa ttgttccgca
     8701 ttcatctctc caagtccttt atatcgctgc agcatatatc ctttgccgac ttttttaatc
     8761 attgctgcca attcgtcatc tgtccacgca tattcaatga cttgcttctt tccttgtcct
     8821 ttggatactt tatataaagg cggtaacgcg atatatactt tcccagcttc aatcagcggt
     8881 ttcatataac gataaaagaa agtaagcagt aatacttgaa tatgggcgcc gtctgtatcc
     8941 gcatcggtca tgatgattac tttgtcgtaa ttacagtctt cgatagaaaa ctcaggaccg
     9001 actcctgcac caatcgtata gatcatcgta ttgatttctt catttttcaa gatatcttgc
     9061 attttagctt tttcggtatt gatgactttt cctcgtaaag gtagaattgc ttggaacttt
     9121 ctgtctcttc cttgtttagc agatccgcca gcagaatccc cttcgactaa atacaattca
     9181 tttttcttag ggtttcggga ttgtgcgggt gtcaattttc ctgaaagaag agattcgcct
     9241 tttttacgct tcttcccatt tcggctttct tctctggctt tacgtgctgc ttctctcgct
     9301 tctctggctt tgattgcttt acggaccaac atctggctca tttcgctatt ttcctgcaaa
     9361 taaaagccca tttgttcact gatgacgtta tctactacgg tacgagcaac tggcgtacca
     9421 agtttttctt ttgtttgccc ttcaaattgc agaagatttt caggtacacg gatagaaagt
     9481 acagcagcta acccttctct gaaatcacta ccttccagat tcttgtcccg ttctttcagc
     9541 aaaccaactt ttctcgcata ttcattataa gatttcgtca tcgccgcttt cataccagct
     9601 tcatgggttc ccccatcttt tgtccgaacg ttattgacaa aagataaaac attttctgaa
     9661 taaccgtcat tgtattgata agcgacctct acttcgatac cttctttttc tcctgaaaaa
     9721 taaacaactg gtgtcaatgt atctttttct tcattcagat aatcaacaaa ttctttgatt
     9781 ccttcttcgt aatgaaaaat ttccttgact ggttcttctc cacgaagatc agaaagagag
     9841 attttaacgc cttttagaag aaaggcagat tctcttaacc gttcggctaa aatttcatat
     9901 gaaaatttcg ttgtactgaa tattgtatca tctggtaaaa aatggacagt cgtcccgttt
     9961 gctttttttg tttttcctat ctttttcaaa gtaccgtcag gtttaccgcc gtttttaaat
    10021 ttctgctgat actctacgcc atctctaacg atcgttacaa ttagccattt cgataaggca
    10081 tttaccacac tggctcctac accgtgcagt ccaccagaag ttttgtaccc tccttgacca
    10141 aactttccgc cagcatgcag cacagtaaaa atcacttcaa ctgttggtat tccggaagca
    10201 tgcattccta caggcatccc acgtccgcta tctgcaacag ttatactatt gtcttcatgg
    10261 atggtcacat caatctcact gccataacct gataatgctt cgtcaactgc attatcgacg
    10321 atttcataga ctaaatgatg caagcctcgg ctgtcagtgg atccaatgta catccctggt
    10381 ctttttctaa ctgcctctaa tccttccaat acttggatgg acgagtcatt gtattcgttg
    10441 tttacttttt ttgccaaggc aaacgctcct tcaataatac tttgcggcaa aaccgcacca
    10501 cgattatcat actagaaaag gtcgtgaaaa aaaagagggt agacgagaaa tcaattttaa
    10561 ttatctccta accgcttttt cgacattccc ctctatcgat catctcttct ttgtggtcat
    10621 ttttattttt ggttttcttt caacttacca agttcaatct taatacaacg atccattacg
    10681 atatctgtac gtcctgcttt tcttagcatc tcagctgctt ctttgttttc aatccctaat
    10741 tgtgcccaga acactttagc atcagttttc aaaaaatcac ctgctatttc aggtaaaaat
    10801 tcactccgtc taaatacatc tacgatatcg attggatcat cgatttctgt caaattcgca
    10861 tagactttct cgcctagaat ttcttcacca gccaataatg gatttactgg tatgatttta
    10921 tatccattgt cttgcaatac ctgtgcaacc atgtaacttg ttcgttctgg tttattgctc
    10981 aagcctacga cggcaatatt ttttgcttct tccaaatagt tgaaaatcgt ttcttgtgtt
    11041 ggatttttta cagacatttc tgccacccct tgcttttctt tttattccat ctattatact
    11101 tgtatctttc attttttcaa ttgttcctgt tacctattca tgctaaaata aaggatagtt
    11161 ttcaacttga aagagggtaa aaaatgaaat tgatcttatt acttatttta gcatatcttt
    11221 taggttctat tccttccggt gtctggattg gcaaattgtt tttcaaaaaa gatatccgac
    11281 aacacgggag cggtaatact ggaacgacca atacatttcg tgtattaggc aaaacagccg
    11341 ggattgttgt cctgcttatg gatattttaa aaggtacatt ggctacttgt ttgccgatga
    11401 tcttccacct ttctgggatt aaccctcttt ggtttggggt atgcgccatt cttggccata
    11461 catttccgat ttttgctggg ttcaaaggag gaaaagctgt tgcgacaagt gcaggaatgc
    11521 ttttaggatt taatcctgta ttttttgttt attcttgtat catctttatc gttagtttat
    11581 tttgtacaag tatggtgagt ttgagcagta tgatcagtgc agtattgatc actttatcta
    11641 cgatcgtcct tccttatgta gctcctgtga ttttggctaa gcccaattgg ttactgacga
    11701 tcattgcctt tttagtttct tcctttatct tttatcggca taaagacaat atcaagcgta
    11761 tcagaaacca tactgaaagt cgaatcccgt ttggtttgaa tagcagcaag aaaaaatcct
    11821 gatacttttt cattccaaag agataaaacg ggagtgtgac aaatcgatgt cacactccct
    11881 tattacttac aggatagctt ttttacacac cttggtaaac ggtgttccct gctaacttct
    11941 cggtcacaac ctcttacttc actgtgatgg aatattttgt cttgaagatt tcatgggcac
    12001 ctagttgatt gatccctact ttttccatca agtttccact actgttcacc gtgtcagcga
    12061 ttccgcacca cggttcgata caaacgaaag gcgcttcttt tggataagga gaccagatcc
    12121 ctacataagg catatcttta tagcttaaag ttacgctatg ggaagatttt tcacttcgaa
    12181 tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc atcttgctca aaaagttcac
    12241 gattcaaagc cagattcgta tttgtctgac ctaacgtctt ttgatccata tcgatgaatg
    12301 ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga aaatgccaga tagaaatctt
    12361 caaatgacaa tccttcttca agaggtacat tgaaagcagg atgtccaccg atagaaaaga
    12421 acatttcttt ttcaccggta tttgccactt gataatgaac cgtgatgccg tctcctccta
    12481 attcatagct gatcactaat tcaaaatcaa atggatactt ttctaatgtc tccggcgtac
    12541 tccttaagat gaaactagcc atttcttgtc catgttcaat gacttcaaaa tccatatccc
    12601 gagcaaatcc gtgttgcccc atttgatatg ttttcccttg ataaacatac tgatcatctt
    12661 ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct gccccaatac gctggatcac
    12721 cttgccagat atactctaaa ccatgttctt ttgatttcag actgatcaat tccgcaccgt
    12781 gttcagcgat ttcggcgatc agctggtcat tttgaataat aacagtcatt gccattcctc
    12841 caacttcgac ttacttttta aaatgggaag gctggaacaa acatttctat ctgccccagt
    12901 ccccttactt ctaataactg tgttttgcta actcgtcttt aaaacgggaa ttcaatactt
    12961 ttaaataagt tcccttcatg cctaacgaac gagattcaat gattccagct gattccaatt
    13021 ttcgtaaagc attgacgatc acagaacgag tgatgccgat actatcggca atattagaag
    13081 cagtcagacg accttcatct ccatccaatg cttcaaagat agcttgtaca gccttcaatt
    13141 cgctataaga taatgtgttg attgccattt ggacagccgt tgcactccgt acatctgctt
    13201 cgatgcttcg agacttctga taaaggatct gcataccgac aaccgtagct ccatattctg
    13261 ctaagactag atcgtcatct gcaaacgatt gttccatacg agataagata atcgtaccca
    13321 aacgttcgcc agctccaaag atcggaacga cagtcgtcag accaaatgga tatttctctc
    13381 ttgactctac cggaaatgca gtaatatcac tagtaattgg gatattagct tcagttttca
    13441 tcaactgtgc cactgtatca gtataagatt caggaaattg tttctcaaca aacattgttt
    13501 tgattcgttc attgttcaca tcgtgacgtt cattataacc tagtaatact ccgtcacttc
    13561 ctatgatata ggtgttgctg tcaagaatac cacctagtgt gacagccatt ttatcgtaag
    13621 gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa aagtgtgttt atcttgcggg
    13681 ttttcgttag caagtctgtc attgtcgtcc tccgattttt tataaaatat agcgacttaa
    13741 atcttcattt gccgcaattg agccaagttt gtcatccaca tatgcttctg tgatcgttat
    13801 ttctcccatt tgcatatctg gtgcttcaaa taatagatct tctagtagtt tttccaaaat
    13861 ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg ttgacatcga aagcgatttg
    13921 agcgatccgt tcaattgctt cttttgtaaa cgtaactgac acattttctg taccgatcaa
    13981 cgcgacgtat tgttttacta aagcattatt tggttcggtc agaatcttca caaaatcttc
    14041 agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt ccttgaagtt ctggaattag
    14101 atcacttggt ttagacaaat ggaaagctcc tgaagcaatg aataggacgt gatctgtttg
    14161 gatgatcccg tattttgtgt ttacttgtga accttcgaca attggcaata tgtctctttg
    14221 gacgccttct ctggatactt ctccagattg ctgtgattta gaagtgatct tatcgatttc
    14281 atcgataaaa ataattccac tttcttccgc taatttgatt gcctcactat ggatatcagc
    14341 atccttgact aattttgctg attcttcctt gatcaataac tctaaagctt ctttgactgt
    14401 taccgtccgt tccacttttt tcttcggtgt caaagcacct agagtttcat ttaggtcgat
    14461 ccccatttgc tccaatccat tgttcatcat tggtgcaggt ttcttttggt cttcaacttc
    14521 aatggttact tcacgactat ccagtaaacc cttttgtagt tgttccaaga ctgtacggcg
    14581 attgattcga atatcttctg tcacttcttc ttgcggttca gcggtatttt gctgactagc
    14641 attaaaaaga ttcatcattt gttcaaattg attagtggat tgtttctgtt cttttttgat
    14701 accaggtacc aagattttta ccaaacgttt ttctgcattt tttttagcac ttgtgtatac
    14761 acggctgtac tgctcttttt ctacgatttg gatcgcattt tctactaaat cccgaaccat
    14821 tgattctaca tctcttccga cataaccgac ttcagtaaat tttgttgctt cgactttgac
    14881 aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt tctgttttac cgacccctgt
    14941 aggtccgatc atcagcatat tttttggtgt aatatcttgt tgcatttttt catctaactg
    15001 caaacggcga tagcggttac gcaaagcaac ggcaacagat ttttttgctg cgtgctgtcc
    15061 tacgatatat tcatctaatt cacttacgat ttgtcttggt gtttgtttta attcatacat
    15121 atctactcac ctcttacagt tcttctacga taatattatg attagtaaag atacagatat
    15181 ctgcggcaat atttaacgca ttttctgcaa tttctttcgc actcatgtcg ttttgagcat
    15241 agtttttcat tgctcgggct gcagccaacg caaaattccc tcctgatccg atagctaaga
    15301 taccatcatc tggtgtgata acttctcctg tacctgaaac aagaagcatt tctttatcat
    15361 tcattacgat cagcatcgct tcaagttttt gcatagattg ctgtgtccgc cattcttgag
    15421 ctaattcgac agctgcgcgt tgtagattcc cgttatattc gtttagtttg ccttcaaatt
    15481 tttcttctaa agtaaacgca tctgcaacac ttccggcgaa acctacaaca acttctccat
    15541 tgtagatacg tcgtactttc tttgcagtgc ctttcatgac aacagattca cccattgtta
    15601 cttggccatc tccagccata gcaaatttcc cgtctttttc gacggcacag atagtggttg
    15661 aatgaaatgg tgattcgacc atgtttgttt cctcctaaat ttgttctttt ccttattttt
    15721 acgctcttgg atggaaagaa cgatagtttt tttgtaaact ttcttttgtt acatgggcat
    15781 agatctgggt agtcgataaa ttggcatgtc ctagcaattc ttggactgtc cgcaaatctg
    15841 ccccattatt taataaatga gtagcaaatg tatggcgcag catgtgagga tggatatcac
    15901 tatccaaact gctttttttg atcatttgat tcaagacata ttcaattcca gtaggcgtga
    15961 ttggttttcc atgatgattc acaaaaacga tatcatgctc ttcgtgatat tttgtcatca
    16021 atacctggcg gccttccctg aaatattctt gcagagcatc ttgagcaaaa gaacctagag
    16081 gagcatatct ctctttgttc cctttaccat ggatcaacat gacctctgaa tcaaaatcaa
    16141 tgtctgatac cgacaaactg ctacactcac ttaaacggat gcctgacccg taaaggactt
    16201 ccagtaaagc gcggttcctc aaatccaaag gactatctcc tgctacgctg tcaaaaagtg
    16261 cttgcatttc attttcataa aaaaaacgtg gcagtctcaa atttttcttt ttgagatgaa
    16321 cgtaagaaaa tggattttct tttatcactt cttctttgag aagatactga taaaacgaac
    16381 gtaggctggc tatcttgcga ctgatagaat ttctgctata gctaggacga tgatcagcta
    16441 atgcactcaa atagacacga accgtaaaat attccgcctt cagcagatca gcgtctccag
    16501 attcctccaa aaaatgtttg aaatggagaa tgtcttcttc atatgcttct ttcgtttttt
    16561 ctgaatatcc tctttcgaca atcaaataac gcaaaaattt ttgttcccac gtggcttctg
    16621 tcattttctc accttccttt tgtcccagag aataatttag cacagcgttt ataaaaagac
    16681 aaataaattg tcagaattta accaatattt ctaagtttgt tcacaattgg cacaattcct
    16741 cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa aagagcgaga ctatccgtct
    16801 caccctcttc atggagtttc cttactcatt tgggattgca gttttttcta attctgtttt
    16861 tattgcctca ttagctttta aagctcgttg tgcaattgct tcatatcttg cttttttgtc
    16921 tcgaatgcgc tctggcagtt caggaaacag accatagttg gcattcatcg gttggaaatg
    16981 ttttccttcc gcatgagtaa tgtagtaagc catacttcca atagctgttt cttgtggaaa
    17041 ctcgactggt tccatttctt ttgctaagcg tgcggcgttg attccagcaa gtagtccgct
    17101 cgctgcactt tcaacatagc cttctacacc agtcatctga cctgcaaaga acagatcttc
    17161 gcgtttcgtt gattggtaag tcggtttcag caattctggc gaattcataa agctgtttcg
    17221 gtgcatcaca ccatagcgaa cgaattcagc attttctaac ccaggaatca tccggaaaac
    17281 acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc acgatgttgt ataaagaagc
    17341 tgcagcattg tcttgacgca gctggataac agcataagga cgtttgccag ttttaggatc
    17401 ttccaatcca actggtttca taggtccgaa aagcatcgtt ttgaatcctc gctgtgccat
    17461 tacttcaatt ggcatacatc cttcaaaata tttttctttc tcaaaagatt taagttctgc
    17521 cacttcggca ttgattagtg cctcatggaa agctttgaat tcctcttctg tcatcggaca
    17581 gttcaagtaa gctgcttctc ctttgtcata gcgggacttc aaatatactt tatccatatc
    17641 gatcgtagct ttgtctacga ttggtgcagc tgcatcatag aaataaaaac catctgaacc
    17701 attgaatgct ttgatgcttt ctgctaatgg atgagaagtc aaaggtcctg ttgctactac
    17761 ggtgattcct tccggtattt cagtaatttc ttcgtttatc actgttacta atggatgctc
    17821 tttgactttt cgggtaattg tttcagaaaa agaatcgcgg tcaacagcta aggcaccacc
    17881 agctggcact gctgtttcat ctgccgaagt gattactacc gaattcaatc ggcgcatttc
    17941 ttctttcaat aaccctactg cattagccaa actgtttcct cttaatgaat tggaacatac
    18001 tagttcagca aaattacttg tgtgatgcgc ctcagtcgat ttgactggac gcatttcata
    18061 taaacggaca gggactcctg cttgtgcgat ctgccaagca gcttcacttc cggcaagccc
    18121 tgctccaaca acatttacca ttttcaatga aagaacctct tttctattat ttttggatgt
    18181 cttcttcgta atctccattt acacagacta cttgtttacc gcctttgact ttcttctcga
    18241 ccagatatcc gtcacatttt ggacaatttc tgcccactgg tttatcccaa gaagtgaatt
    18301 cacattctgg gaaacgggaa caaccataga atagtctgtt tttcttagat tttcgttcaa
    18361 tgacttgtcc ttcatgacat actgggcaag tcacaccgat ttcttttacg attggtttcg
    18421 tattccgaca atcaggaaaa ttactgcaag cataaaactt cccatatttc cctaatttta
    18481 tcaccatcgg atgtccacag acatcacaat cgaatcctgc tggttcatct ttgatttgaa
    18541 ttttttcgat tttttcttcc gcattcgtca gttctttttc aaacggttta tagaaacgat
    18601 caaccacttt gacccacgct tccgtgcctt cttcaatttt atccaaatct ccttccattt
    18661 ctgctgtgaa atggatatcg acgatttgag ggaagaattc acagattaat gaattgacaa
    18721 tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa ttttacatag tagcgtcttt
    18781 ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc taccccgttt tcttctagtg
    18841 cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg agtaaaatgt tgttttggtt
    18901 caatatcaac agattggact acatcgcctt cttcaagttc tggaagaata ttttctttct
    18961 cttctttgcc gtcatctcta ccttccacat acacttgcat gaatcctttg aatttgattt
    19021 ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa tgtcactttc attgtatcta
    19081 aaatagctgg tgtcatctga ctagccacaa aacgtgacca gatcaatgta taaagtttca
    19141 attgatcttt atctaaatat tgtttgagtt catctggtgt acgaagcaca ctcgttggac
    19201 gcacagcttc atgggcatct tgtgcgcctt gagcattttt gatctttcgg tgatcatgtc
    19261 tagaaaattc ttttccatac gtatcttcaa taaatgctgc ggcctctgct tttgctgtat
    19321 cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa gccgactgtt ccttgtttac
    19381 ccaaagtgat tccttcatat aattgctgtg ctaccatcat ggtttttctt gttcgaaagt
    19441 ttaacttacg ggcagcttct tgttgcaggc tacttgtagt gaaaggattc gcagggtttc
    19501 ttttgcgctc tttcttttct acttttttaa cttcgaagtc tgttccatca attctagcag
    19561 tgacttcttt tacactttgt gcatctggta atttcttttt cttaccgtcg attccccaga
    19621 aatttgcttt gaatttcttg cgagcttttt taaaattacc gtcgatgctc caatattctt
    19681 ctggaacaaa ttctcggatc tctttttcac ggtcaataat aatttttaaa gcgacagatt
    19741 ggacacgtcc tgcacttaac cctttcttga ccttacgcca gagaatagga ctgatcgaat
    19801 aaccaacgat tctgtctaag gtacgacgag cttgctgtgc atctactaaa tctacatcga
    19861 tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt tgtgatttca ttgaagacca
    19921 cccgattttt ttctttcaga tccaacccaa gtaggtacgc taaatgccaa gcaatcgctt
    19981 ctccttctct atccgggtca cttgccaagt aaactttttg agcttttttt gctgcggctt
    20041 tcaggctttt gatgacatcg ccttttccgc gtatagaaat ataatggggt tcataattgt
    20101 tttcaaaatc gatacccatt ttacttttcg gtaaatctct tatatggcca acactggcca
    20161 ttactttata gtttcgtcca agatatttct caattgtctt ggcttttgca ggtgattcta
    20221 caatcactaa atatttatac gccattactg aatggactcc tttccattgt cgtgttccta
    20281 ctattaaaag aggattcata caaatcgtag ctaatcatat ccattcagaa aacgtttgtc
    20341 aagaaaagca aatgaaaagt atggtgttat tctttaaatt ctgccaagat atcttcaccc
    20401 gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac atccggtaga atgttctgtt
    20461 aaaatatttc ctggaacaga aaaaactgtt cttccaagat ccaatgcttg ctgtgctgtt
    20521 atcagagagc cgcttttttt cttggcttca aagacacatg ttcctttaga caaagctgcg
    20581 ataatccgat ttctcattgg aaaacaatgc tttttcgggc cggattgagg aggatattca
    20641 ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt cacgattttc agcaggatag
    20701 acttgctcta tccctgttcc aatgactgcg attgtccgtc caccatgaga gattgctgct
    20761 tggtgaccgt atgtatctat ccctttcgcg cagccactga cgatcacata tccagcttct
    20821 atcactggtt ttagtaaata ataaactgtt tttttggcta aagcagaagt gtgtcttcca
    20881 ccaacaacgc ttaacatgtt acaagaaagt aaagatagat caccattata aaacagtaca
    20941 attggcggat aggtcaagtg aagcagctga gggggatatt tgggatcaaa gcaagtgata
    21001 aacgactgtt tcgattggat tttgccccag ttagcgtgaa tttcctgcca acttttgcga
    21061 aacagctccc ggtgtttggt tattttacct atcttaatga tgtcttcaaa tccgatttct
    21121 ttataaggat actgcatcat ccagttagca agtgcccatt ttccttgact actgattcct
    21181 tttgtataag caagtttcaa taaattttct tctatttgat acatcataaa accgcctttc
    21241 gtctctcttt ctaataagat acgtaaaagc ggctgttttt tattgtaaat atttttttac
    21301 aggtgcaaaa cttttacgat ggattggcgt aattccttgt cgttcaagtc cttctaagtg
    21361 ttcctttgtt ccataacctg cattatgcga gaaaccatat ccaggataaa gcacgtcata
    21421 ttctttcatc aattggtctc gatatacttt agcaatgata cttgcagcag caatcgacac
    21481 agatcgtgca tcccctttga ttattttttc ctgctgaata ggcaaatcca aggtcatcgc
    21541 atcgatcaac aaatagcttg gtacaatgga caatttttct actgcttgga tcatcgctaa
    21601 tttacttgct tcataaatat tcacatcatc gattgtctgc tgatcaacga tcccgatccc
    21661 gattgccagg gctttttctt gaacttgttg ataaagaagt tctcgctttt tctctgataa
    21721 ttgtttggag tcattcagcc ctaatatgat ctcatttttc ggcaagataa ctgcagctgc
    21781 cacgacaggt ccagctaatg gtcctcttcc tacttcatca atgccggcaa tccattggtg
    21841 accttgtgcg tacccttgct tttcaaagca aaacatttct tcatattttt ccttaattaa
    21901 tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct tgctgcacgc ctttgcgtgg
    21961 atcatttctt aatgattcta gtgtaggatc atctattctc tgtatgcttt tcaatcgttc
    22021 tttgatactt tggatggatt cagtcatcac ttttttgctc catttcttcg caacgatcca
    22081 gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac gactaattcg ctgccacgat
    22141 catagtcttc tttataccca cgttttgctg taatatccat cagtaattcc ggtgccaatt
    22201 tttcttcttg ctcgattgat aaaccataac gatcgctgat tcttcctgaa taatatcggg
    22261 caaaaaagtc taatccgtaa atcgctaaat catctaaatg gacaagctgg tctttgatcg
    22321 cgcctgtcag cgctagtttt ttgccgattt cttcatcttc aaatttgggc cataaaatcc
    22381 ctggtgtgtc taacaattcc aactcattac ctaaacgaag ccactgctga ccttttgtta
    22441 cgccaggctt atttcctgtc tgtgcaattt tcttgcctac tagtcgattc atcaacgtag
    22501 atttcccaac atttggaata cctaagacca ttgcccgaat agcgcgaggc ttgatccccc
    22561 gagcagcatc tcttgcccgt ttttctttca atgcttcttt agccgcaggt actagacctt
    22621 taattccttt actttcttgt gccgtgatag ctaatgcaag atggccttgt ttacgaaaat
    22681 attcttgcca cagctttgtc tgttgtggat ctgccagatc cgctttattc aataaaacaa
    22741 ggcgaggttt ttgctgaatg atctgatcta gcattggatt acgagaagat aaaggtaatc
    22801 gtgcgtctac caattcaaat acgatgtcca catatttcat tttttcagaa acttctcgtc
    22861 gggcttttgc catatgtccc ggaaaccatt ggatggtcat tgttctctcc attcctttca
    22921 taccggaata ttccccggct ttacttgttt ttttcttttt caatataaat tcactttttt
    22981 attataaaga aattccatct attagttaag tgaaagtgaa ccagtcctca ttctatcaca
    23041 tcttcacttt tttgatgatg caaattttta ttttggtttc tatatatgaa tctaaatttt
    23101 tgacagcgtt tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa
    23161 tgaatagtat ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt
    23221 atgaaaagct gggttttaaa aaaaatgaag aattttctac tgaagaagct ggttcaatgg
    23281 tatgggatga cactttttgg ataatgcttc tctctcatga tttttataaa aaatttataa
    23341 aagataaaga aatagctgat aataaaaaaa caagtggtgc tttgatttcg tttagtgttg
    23401 aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa agaaaatggt ggtactttcc
    23461 acgaagtaga tatgggaatg cctgaagaag aaatgttttc attagaagtc caagatctag
    23521 acggaaatac attagaaccc gtatggatga agatgtaagc agataactaa tctgacgttt
    23581 tttataacaa aaaaacaaag gcggatgata cgataagtat tctccgcctc tatttttttt
    23641 attcgtacaa tctctttttt acagtttata aaagcagtct tctcaccctt tttttaattg
    23701 ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca cattggtgat tttatggtat
    23761 ccttttatta ttaactgaca aaggagagaa tattttgaac actttgattg caaaagacgt
    23821 ttttagattg cccgaaatga atgcaagatt gttgtcgacc tctgctcttt tattagccct
    23881 tgatgtcatc ttatacaaga tagcgattgg tcctgcttat tttcaaatca cattcggatt
    23941 tttaagtatg gcgcttatgg gttatctata tggaccgatc tgggctggtt tgcttgaaat
    24001 gttgtcgaac actttaaatt tcacgatatt tggttcagga acgtttcaag ctgcttttct
    24061 tatcacggct ttccttggtg gatttattaa tggcttattt ttatatcata aaaagataaa
    24121 cctgccttcc gtcatcgctg cccaattatt gattatgatc ccagtcagcc tttttatcaa
    24181 ttcttggctg atgtctgttt tgtttggtgc agatttcaaa gtaatttttc taggtagatt
    24241 catcaggaat atgatccaaa tccctattca aataatggtt gtttattcat ttatcaaaac
    24301 tttagaggct agaaaagtgt ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac
    24361 tgattttgga ttaaacaata aagcgtgcag tctcaacaga ctgcacgctt tattgtttct
    24421 cacaggatcc attttatttc atcaagtgga taatagacaa atcttgcttt acctagaata
    24481 tcagttttat tgatcgtgcc aaacgaacga ctatcttttg aaactcttcg attatctcca
    24541 agtacaaaat aattatcttc cggaagcttg gattgtcccg ttagctcttg tagtgtaaag
    24601 tccgtcgtat aggatgcatt tgcatgatct gatttcaggt ttttagttag ataaggttcc
    24661 ttaactactt tcccattgat cttcaattga tcattttggt agctgacatt ttctcctgga
    24721 agaccgatta ctcgtttgat ataaatcgtg ccatctgcta attgaaaaac aacaatatca
    24781 aatcgtctga tctccgaaaa tttttccatc acaaccatat caccttgctt caacacgttt
    24841 tccattgaat tgccttccac tggtaccggg atgaataaaa atccccgcaa catgaacgca
    24901 aggaatacag aaacaagtaa gtatttgaat acaagccaga agcgattaat atagcgttgt
    24961 ttttttgtca atctctatca atccatccct ctttttttca ttctttcttt attataagaa
    25021 aaatatcaaa aaaatgcgac tagatagctg aaataaaaat aattctttca attaggctat
    25081 tggacaaaaa acttttttga tcataaagta caaaactatc tcttatatgt catgattatt
    25141 ttctaaatat ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt
    25201 cagttccaga taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc
    25261 tttaccggct tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt
    25321 cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag
    25381 gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga
    25441 tggtcttctt ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt
    25501 tcgaaacgtc cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct
    25561 tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa
    25621 gcgtcagttg gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat
    25681 gttggatcat agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt
    25741 tcataagtcg gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca
    25801 ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt
    25861 tccattatga ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt
    25921 gctttcagta ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt
    25981 atcttttggc aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc
    26041 tttaatatac atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc
    26101 atctgtattg acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac
    26161 atctttgatt ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt
    26221 tccgttttcc atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac
    26281 ttcgaaagta ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact
    26341 atctttttga tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga
    26401 attgatcgtt aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc
    26461 tggttcccga ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc
    26521 aagaaggata aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac
    26581 gtcagatctt gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc
    26641 tcgatcagct gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg
    26701 aaatgatctt cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta
    26761 gttgtttgat ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc
    26821 aaatcattga ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct
    26881 ttgttccaat tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat
    26941 ggattgtaga tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc
    27001 aatagttctg tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt
    27061 aacccgaata aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg
    27121 ataaaatcag ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag
    27181 atctggtcac ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta
    27241 taacggtctt ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct
    27301 cctactcctt cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt
    27361 ggatcttctc tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca
    27421 gctaaacctg catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt
    27481 cgtttcgtta atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa
    27541 ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa
    27601 gaaaaagaga ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc
    27661 tacataatac atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt
    27721 atgcagtaca gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg
    27781 cgccaattct tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt
    27841 cagttcattt ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt
    27901 tacagggatt aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga
    27961 cagcaaacca gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat
    28021 tccgatgtat aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac
    28081 tgttttacct tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc
    28141 agtggtatca ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc
    28201 tgcttctact gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc
    28261 ttctcctagc tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg
    28321 cagatttttt gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc
    28381 gggatatact gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc
    28441 gcgtacattt ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat
    28501 tttctctctc tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat
    28561 ttctattttt agtataacag acaaataatg atttttcatt aattacacct agtaaaggag
    28621 ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta
    28681 atgcagtcac ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag
    28741 taaaaggtgc ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga
    28801 tgatgattct tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa
    28861 aaaaggtgtt ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata
    28921 ctcctttttg tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct
    28981 ggttgtttgc gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag
    29041 tatcaaaagt atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg
    29101 ttcctcttta tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt
    29161 cttacacgat aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt
    29221 ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt
    29281 aataaaaaaa ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc
    29341 ttttttatat cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg
    29401 tttcaaattg atgcggataa cgattttttt catccatttc tccttgacta gtattgatca
    29461 gtgaccaatc agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga
    29521 tgactgtacg gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc
    29581 cagcgatgaa aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat
    29641 gcatgacatg cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac
    29701 gattcggcaa cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat
    29761 ttgcttctgt catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt
    29821 cttcttttcc aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat
    29881 cctttctgat tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct
    29941 aattggatat cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa
    30001 gtcggaaatg cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata
    30061 taaagatgag catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt
    30121 gcaattaggt gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca
    30181 tccccactgc gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac
    30241 atcgtatgac aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg
    30301 atcaatcttc tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg
    30361 ataaagccac cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga
    30421 tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc
    30481 tgctttttat attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc
    30541 atatctggtc cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa
    30601 atatggtttt tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc
    30661 aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt
    30721 tgcagatcaa accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct
    30781 tctttacgat gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc
    30841 ctttttcctc ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt
    30901 caagttgtgc ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat
    30961 ctccttgatg attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct
    31021 catcttcaat cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag
    31081 gttccttgct ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct
    31141 tcagtaaata actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata
    31201 ctaataactt atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag
    31261 cacctggaaa acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat
    31321 tggtcggttc atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata
    31381 accggcgttt ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa
    31441 ataaaaatcg ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt
    31501 cttcagcagc ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt
    31561 cttgtgtata atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg
    31621 gaattctgcc agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa
    31681 tacctagtct ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat
    31741 ttatacggta actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta
    31801 ggttcagctc taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga
    31861 aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag
    31921 ctaattcctg tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac
    31981 gttctgcttt ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa
    32041 atgaaagttc gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca
    32101 tcaatagtgc gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat
    32161 ctaaatggtt tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg
    32221 ccagactaac tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac
    32281 tgattcctaa tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt
    32341 tattcatttg ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt
    32401 cccctagttc gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc
    32461 cttgaaagac tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat
    32521 aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga
    32581 taccggcaat aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga
    32641 tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag
    32701 tttttttgag gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt
    32761 gtatcataaa aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct
    32821 c
//
LOCUS       DANKHK010000022        32590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000022 DANKHK010000000
VERSION     DANKHK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(41..421)
                     /gene="yajC"
                     /locus_tag="PW109_000934"
     CDS             complement(41..421)
                     /gene="yajC"
                     /locus_tag="PW109_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2510800.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(518..1663)
                     /gene="tgt"
                     /locus_tag="PW109_000935"
     CDS             complement(518..1663)
                     /gene="tgt"
                     /locus_tag="PW109_000935"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2510801.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1881..2579
                     /locus_tag="PW109_000936"
     CDS             1881..2579
                     /locus_tag="PW109_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2510802.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2747..5386)
                     /locus_tag="PW109_000937"
     CDS             complement(2747..5386)
                     /locus_tag="PW109_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2510803.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6005..7036)
                     /gene="queA"
                     /locus_tag="PW109_000938"
     CDS             complement(6005..7036)
                     /gene="queA"
                     /locus_tag="PW109_000938"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2510804.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7229..8404
                     /locus_tag="PW109_000939"
     CDS             7229..8404
                     /locus_tag="PW109_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2510805.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8408..9049
                     /locus_tag="PW109_000940"
     CDS             8408..9049
                     /locus_tag="PW109_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510806.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9046..9963
                     /locus_tag="PW109_000941"
     CDS             9046..9963
                     /locus_tag="PW109_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2510807.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            9960..10631
                     /locus_tag="PW109_000942"
     CDS             9960..10631
                     /locus_tag="PW109_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510808.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11172..11660)
                     /locus_tag="PW109_000943"
     CDS             complement(11172..11660)
                     /locus_tag="PW109_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2510809.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11861..12127)
                     /locus_tag="PW109_000944"
     CDS             complement(11861..12127)
                     /locus_tag="PW109_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2510810.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12127..12390)
                     /locus_tag="PW109_000945"
     CDS             complement(12127..12390)
                     /locus_tag="PW109_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2510811.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12866..13051
                     /locus_tag="PW109_000946"
     CDS             12866..13051
                     /locus_tag="PW109_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2510812.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13193..15016)
                     /locus_tag="PW109_000947"
     CDS             complement(13193..15016)
                     /locus_tag="PW109_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2510813.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15614..18319)
                     /locus_tag="PW109_000948"
     CDS             complement(15614..18319)
                     /locus_tag="PW109_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2510814.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18625..19572)
                     /locus_tag="PW109_000949"
     CDS             complement(18625..19572)
                     /locus_tag="PW109_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2510815.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19762..20031
                     /gene="rpsN"
                     /locus_tag="PW109_000950"
     CDS             19762..20031
                     /gene="rpsN"
                     /locus_tag="PW109_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2510816.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20207..20569)
                     /locus_tag="PW109_000951"
     CDS             complement(20207..20569)
                     /locus_tag="PW109_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2510817.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20580..21701)
                     /locus_tag="PW109_000952"
     CDS             complement(20580..21701)
                     /locus_tag="PW109_000952"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2510818.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21717..22067)
                     /locus_tag="PW109_000953"
     CDS             complement(21717..22067)
                     /locus_tag="PW109_000953"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2510819.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22197..23708)
                     /locus_tag="PW109_000954"
     CDS             complement(22197..23708)
                     /locus_tag="PW109_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2510820.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24030..25382)
                     /locus_tag="PW109_000955"
     CDS             complement(24030..25382)
                     /locus_tag="PW109_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2510821.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25535..26611)
                     /locus_tag="PW109_000956"
     CDS             complement(25535..26611)
                     /locus_tag="PW109_000956"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2510822.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26807..28552
                     /locus_tag="PW109_000957"
     CDS             26807..28552
                     /locus_tag="PW109_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2510823.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28832..29269)
                     /locus_tag="PW109_000958"
     CDS             complement(28832..29269)
                     /locus_tag="PW109_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2510824.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29253..30626)
                     /locus_tag="PW109_000959"
     CDS             complement(29253..30626)
                     /locus_tag="PW109_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510825.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINIGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(30948..31436)
                     /locus_tag="PW109_000960"
     CDS             complement(30948..31436)
                     /locus_tag="PW109_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510826.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31458..32060)
                     /locus_tag="PW109_000961"
     CDS             complement(31458..32060)
                     /locus_tag="PW109_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2510827.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            32159..>32590
                     /locus_tag="PW109_000962"
     CDS             32159..>32590
                     /locus_tag="PW109_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510828.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAE"
BASE COUNT         9362 a         6930 c         6028 g        10270 t
ORIGIN      
        1 atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt
       61 ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt
      121 ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt
      181 tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt
      241 tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac
      301 ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg
      361 acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca
      421 tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata
      481 acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt
      541 tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga
      601 ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca
      661 aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt
      721 ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg
      781 catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag
      841 taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag
      901 cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt
      961 ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac
     1021 cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg
     1081 ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc
     1141 gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga
     1201 atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga
     1261 ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc
     1321 cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg
     1381 ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt
     1441 cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt
     1501 ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca
     1561 ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt
     1621 tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct
     1681 atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc
     1741 cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca
     1801 aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta
     1861 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
     1921 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
     1981 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
     2041 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
     2101 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
     2161 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
     2221 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
     2281 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
     2341 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
     2401 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
     2461 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
     2521 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
     2581 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
     2641 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
     2701 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
     2761 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
     2821 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
     2881 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
     2941 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
     3001 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
     3061 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
     3121 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
     3181 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
     3241 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
     3301 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
     3361 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
     3421 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
     3481 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
     3541 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
     3601 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
     3661 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
     3721 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
     3781 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
     3841 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
     3901 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
     3961 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
     4021 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
     4081 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
     4141 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
     4201 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
     4261 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
     4321 tcgttacttc atcttctttg ctccatactc ggcggacgcg catttcattt tgtgtcagcg
     4381 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
     4441 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
     4501 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
     4561 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
     4621 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
     4681 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
     4741 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
     4801 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
     4861 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
     4921 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
     4981 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
     5041 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
     5101 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
     5161 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
     5221 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
     5281 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
     5341 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
     5401 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
     5461 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
     5521 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
     5581 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
     5641 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
     5701 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
     5761 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
     5821 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
     5881 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
     5941 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
     6001 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
     6061 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
     6121 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
     6181 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
     6241 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
     6301 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
     6361 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
     6421 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
     6481 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
     6541 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
     6601 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
     6661 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
     6721 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
     6781 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
     6841 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
     6901 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
     6961 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
     7021 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
     7081 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
     7141 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
     7201 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
     7261 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
     7321 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
     7381 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
     7441 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
     7501 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
     7561 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
     7621 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
     7681 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
     7741 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
     7801 tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca tcatgagtga
     7861 aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg caaacgattt
     7921 cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg cgacaagagt
     7981 cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat tacaagaagc
     8041 aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata attcgaatgt
     8101 attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag tatccagtgt
     8161 cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc ttcgtgatac
     8221 cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg aacagaacaa
     8281 agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg tgatttgggg
     8341 agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat ctaaggaggt
     8401 ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa aaggttttga
     8461 acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg taccgcttgg
     8521 tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa ccagtgcctt
     8581 gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg gcgtaggtaa
     8641 attacctgcg attatcgcac tctttattta ttcattattg ccgattttac gtaatacgta
     8701 cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca tggggatgac
     8761 tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga tcatggctgg
     8821 tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt atatcggcgc
     8881 tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag atctcatttt
     8941 ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc taggtctctt
     9001 ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa acgtatacta
     9061 aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt ccctggtctt
     9121 gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc agaagcacag
     9181 atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa taccgcaatc
     9241 atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg agatgcttca
     9301 atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct tcctcctgaa
     9361 aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag atacggtcaa
     9421 acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg aaaagacacc
     9481 gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga tgaattagta
     9541 gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg cttccaagaa
     9601 acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt ttatgacgca
     9661 gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg gatcgccagt
     9721 tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga tgctgctcca
     9781 gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt aaataagtta
     9841 ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga taataatcta
     9901 gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt tgaagggaag
     9961 tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc caggaaaatg
    10021 gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat ggtgtcctct
    10081 ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt aaattagcaa
    10141 actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc atgatctcca
    10201 ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta tttttgtatt
    10261 ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac aaaaacatcc
    10321 ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg gtagagttac
    10381 cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt gccatcggga
    10441 taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt atccgaggaa
    10501 ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact gcattaatgg
    10561 cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca gccagccgta
    10621 catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt ggtcgcaaag
    10681 gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc ctccttatgg
    10741 gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt catactgcca
    10801 acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc
    10861 aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct
    10921 gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc
    10981 ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac tggtgactgc
    11041 cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc
    11101 taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg
    11161 gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg atgatccttc
    11221 accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg
    11281 gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa
    11341 agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg
    11401 gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt atcttttcca
    11461 aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt
    11521 gcttttttca acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc
    11581 tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt
    11641 ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc
    11701 attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat
    11761 catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag
    11821 actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg
    11881 cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact cgatgctgtc
    11941 tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg
    12001 tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat
    12061 tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt
    12121 tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac tgtcgtcttt
    12181 ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat
    12241 cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat cactaataat
    12301 ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc
    12361 agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat
    12421 agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc agcaaacttt
    12481 tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt ccacgctcac
    12541 ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac aacgtatcat
    12601 ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa tacacgaagg
    12661 ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac gcacgaaaga
    12721 tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc tcaaaaatga
    12781 ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg cttagaccgt
    12841 tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa aatggtcact
    12901 tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg aggtaaagga
    12961 agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa attatgatca
    13021 agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat ttcatacgct
    13081 gttcccatct ttaatggctt atagagggca cctttagctg agaataagac aaaacagacc
    13141 ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg gtttatttct
    13201 tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat cgcaatctta
    13261 accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt acaaattgag
    13321 gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg atatctacat
    13381 aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa aaacggacat
    13441 ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca tcttcgtcta
    13501 gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc aacgcaccga
    13561 tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca taatcatgta
    13621 gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga tcatggatct
    13681 tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg tcccctaacc
    13741 gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta atattggcaa
    13801 tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc cctgtttgag
    13861 ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt tctgttgagc
    13921 catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc ccattggaat
    13981 agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct tttgccagat
    14041 tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct aaaatcagcg
    14101 ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct ttaccaattt
    14161 gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg ataaatgtga
    14221 tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca gcggcatttt
    14281 ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc ccagttaaag
    14341 aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc ggcacagccg
    14401 ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta tataaaccaa
    14461 caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat aggaacgatg
    14521 cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt gtcacagaaa
    14581 tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca gcacctgctg
    14641 agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg gaaatcaagc
    14701 cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca tgaggataag
    14761 catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata acaaaagcgg
    14821 caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg acaacgattt
    14881 gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc aaagcagcaa
    14941 atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct actaacaacc
    15001 tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa gttttatagt
    15061 cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa gtcaataaac
    15121 tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa acgctttaat
    15181 acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa taaaaggata
    15241 gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt ggaagagaga
    15301 tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag ttgtcacaac
    15361 ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac caaattaaaa
    15421 acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca gcttttttac
    15481 aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa aaaaacggca
    15541 aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa cactgatttc
    15601 acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg aatttcgtca
    15661 ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca acaaacaaac
    15721 cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct gcagattccc
    15781 gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg aagaatttcc
    15841 ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact aagatgataa
    15901 tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg atcatcatga
    15961 acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg acatctactc
    16021 ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc attgtcaaaa
    16081 atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg tctttctttc
    16141 ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc cacagacata
    16201 gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa agcgcattgg
    16261 tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct agtttcaata
    16321 atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg tctgctgctt
    16381 tttggatacc agctttgata tttggccagt cattgttgat caagtccgca gcagcgttca
    16441 cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg gtgatttcat
    16501 tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat ttttgctgaa
    16561 tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg acgatggtat
    16621 ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt gcaggcattt
    16681 ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc gttgagctga
    16741 gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca gtcccaatcg
    16801 tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct gtattgatac
    16861 cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc aattcatcaa
    16921 tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg ttttgcagat
    16981 tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat ttcgtatttc
    17041 cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca ttttgttgtt
    17101 gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt tcatcatcag
    17161 tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct gtcgttgttg
    17221 ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt ggtatcgcat
    17281 ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc tgattttcta
    17341 aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag ccttgttttg
    17401 cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg tcgatctctg
    17461 ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca aggatgattt
    17521 ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt tcacccatct
    17581 catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt tccttgattt
    17641 caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc gtgtccaaat
    17701 tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg ttcgtatcga
    17761 tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt ttgataaatt
    17821 catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc ggtgcaatcg
    17881 cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca ccactagtaa
    17941 aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa tactttccgg
    18001 aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct aattgtttat
    18061 ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct tgaaactctg
    18121 ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca tatggatccc
    18181 ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc gcgatgatca
    18241 gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat aatcgaagcg
    18301 tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa ttactttacc
    18361 atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt tggacatatc
    18421 acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga ttacagcctt
    18481 tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta tctattatac
    18541 gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg tttcagcttt
    18601 tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg
    18661 aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag cagacagatt
    18721 cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc
    18781 ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg
    18841 ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat
    18901 ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg
    18961 atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa
    19021 tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc
    19081 atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta tcattacagt
    19141 atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt
    19201 gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga
    19261 tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc
    19321 tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc
    19381 tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac
    19441 atcataagga ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc
    19501 tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc
    19561 aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg cctttatttt
    19621 gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt
    19681 cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa
    19741 aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag
    19801 caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac
    19861 gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgattta
    19921 atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt
    19981 gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct
    20041 ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg
    20101 ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag
    20161 ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag
    20221 gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg
    20281 gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct
    20341 ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc ccaatgtgag
    20401 tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa
    20461 aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc
    20521 ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct
    20581 tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc
    20641 gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata
    20701 agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata
    20761 catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc
    20821 tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga
    20881 gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatc
    20941 aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag tgcgtgccca
    21001 gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca
    21061 tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc gggaagtttt
    21121 ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct
    21181 gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat
    21241 gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct
    21301 gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct
    21361 gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc
    21421 agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc
    21481 aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct
    21541 gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac tgcaaacaat
    21601 tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta
    21661 tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag
    21721 tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccataca
    21781 ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt taaaatctcg
    21841 acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg
    21901 caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt
    21961 ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga
    22021 gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt
    22081 ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt
    22141 ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt ttatgtttaa
    22201 tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt accgccatct
    22261 ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg ataattaccg
    22321 ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt gtatcctttt
    22381 ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc agctggatct
    22441 tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc ttctaataat
    22501 ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt ttcgatttgt
    22561 tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg acgtaaagtc
    22621 gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc atttggtgta
    22681 acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat acggtgaacg
    22741 tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc tgaaatatct
    22801 aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct cttaaatgaa
    22861 cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc tgcacggtaa
    22921 ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg tccgaagacg
    22981 attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa tttttcgtat
    23041 tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt cgctttgatc
    23101 ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc tggcggcatc
    23161 gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat tttttcgata
    23221 tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa tgtttgaacg
    23281 gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg tgtacccaca
    23341 acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc tccgccgtaa
    23401 acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg
    23461 atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg atctggatcg
    23521 attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt
    23581 ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat aggcgttgct
    23641 tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc tagttcttta
    23701 aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt ttgctacccc
    23761 gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc acgtttttat
    23821 ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag gcattagaaa
    23881 agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca acaagttgtt
    23941 tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat tctaacatag
    24001 tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg ttctaccact
    24061 tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc ttcaagattt
    24121 tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac aggtagttgg
    24181 gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata aaggaaaatc
    24241 atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt taaagactct
    24301 ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt tggcagattt
    24361 ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac atcgctcaaa
    24421 atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat agaaacatgg
    24481 gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat cgtatacgca
    24541 atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt ttctccttct
    24601 actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac atcgccattt
    24661 ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt
    24721 tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat ttccattttg
    24781 gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc gatcattgtg
    24841 attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt ggcgtgatcc
    24901 atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat cgtgtaagga
    24961 agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt ttcagacaag
    25021 acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact
    25081 tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt caaaggatct
    25141 gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct ccaaaatgaa
    25201 gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg cgtaccagca
    25261 agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac attggtaatc
    25321 tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat tgttagattc
    25381 attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc
    25441 atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa tgaacgcggt
    25501 tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca aaaaaagatt
    25561 gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa tcaccgtatt
    25621 tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat cctggcatag
    25681 agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg caccggctca
    25741 atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa
    25801 cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata tattttgctt
    25861 cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc cgaacgtctt
    25921 catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca acgattgctc
    25981 gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg ttttctgctt
    26041 ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga taaagcaaag
    26101 aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc ttaagtactg
    26161 gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc atgattttat
    26221 ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc aatgtctcta
    26281 agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga aaaacgatgg
    26341 ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct tcctctgtct
    26401 gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt tctgttagta
    26461 atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact tggtaataat
    26521 tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca tgctctgcgc
    26581 tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt
    26641 cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact tatttataaa
    26701 aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct agattatttg
    26761 tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa
    26821 tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg tagtgccaaa
    26881 ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac taggccccac
    26941 cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt ctgccttcac
    27001 tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa cggtcaaaaa
    27061 gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt tttcaaaatc
    27121 gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc
    27181 ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg atcttcaact
    27241 atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag ttgatctaaa
    27301 aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct taaaagaaag
    27361 tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc gcagccccaa
    27421 gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa
    27481 cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg gaattcaaaa
    27541 caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga aacaggtgtt
    27601 tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc atgtactaac
    27661 aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg
    27721 ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat ataccatcaa
    27781 ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa aactcacaca
    27841 agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact
    27901 ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga caagttttct
    27961 tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat tcacacatgt
    28021 cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc ttttgctgaa
    28081 agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt ccctcatgga
    28141 agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag aagatacatt
    28201 gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga tccctcaaca
    28261 gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt tggaattgaa
    28321 aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg ttttcggtat
    28381 gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa
    28441 cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat tcgtcattat
    28501 ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt agaaaaaggt
    28561 tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat tgtttctcaa
    28621 atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac cgagaataaa
    28681 aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg aacatggaac
    28741 acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata aaataccttc
    28801 gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt aacgcttgac
    28861 gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt atgccagctt
    28921 ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa
    28981 attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt gaagctaaac
    29041 cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt ttataatccg
    29101 cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat tctttggtcg
    29161 tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct cctaatttta
    29221 tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt ctttttcgcc
    29281 ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaat attgatccat
    29341 tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt cagcgtgatc
    29401 aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag cgtcatcaat
    29461 aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac gatccggcga
    29521 tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct gcctgttact
    29581 acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat caagaggacc
    29641 aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt taaacgtttc
    29701 aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg caaatacata
    29761 aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct aaataaaaaa
    29821 aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct tggcaacttc
    29881 cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag caagaaacca
    29941 taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct caaaagaaac
    30001 aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag atttcttttg
    30061 atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta ggccatgacg
    30121 taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg caactcaatc
    30181 gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc atccatattg
    30241 ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac aagccatgct
    30301 gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa tacgcctaat
    30361 attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa cgtgtaacta
    30421 tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa ggcataaaac
    30481 agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa aaataaaaga
    30541 taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt aaaattacgc
    30601 ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt ataactaatc
    30661 tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata tattcttgta
    30721 aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac ttgattactc
    30781 aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat
    30841 aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg taaaccagag
    30901 tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta tgcgagagct
    30961 tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat aagttcttct
    31021 gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc ctgattcact
    31081 cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact
    31141 gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact taaataaatt
    31201 tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa
    31261 aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc
    31321 agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg ccatacttct
    31381 tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac
    31441 ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc
    31501 atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat aaattcccta
    31561 aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc
    31621 agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca
    31681 aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg
    31741 acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca
    31801 aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga
    31861 atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc
    31921 tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga
    31981 atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa
    32041 actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct
    32101 ccttatgatg tgcaccccaa aagttagact agaaatctaa ttaaaagggg tgcttttttt
    32161 gcggaaatat acatttgttt ttaagaaaaa agtagtttca gactatttaa ataacgaagg
    32221 cggctacaaa tatcttgcac ataaatatca aataaatcgt acactggtta gacattgggt
    32281 aaggatttat aactatcatg gttgggaagg cttggttgga ggtggcaaaa gctacactac
    32341 aaaatttaaa cttgatgtta tagaatatat ggaaacaaat ggtctttcta tccaagaaac
    32401 tgctaaaaaa tttaatatcg gttcaaatag aactctaagt aaatggatag agcaatatga
    32461 agaaggcggt gcttcttcac ttgagagcca aaaaaggggc agaaaaatta gtatgaattc
    32521 caagctaaac attcctaaaa aacttaaaga tgagtctctt gaagaagaag ttattcgttt
    32581 aagagcagag
//
LOCUS       DANKHK010000023        31898 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000023 DANKHK010000000
VERSION     DANKHK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            293..1432
                     /locus_tag="PW109_000963"
     CDS             293..1432
                     /locus_tag="PW109_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2510829.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="PW109_000964"
     CDS             complement(1499..2074)
                     /locus_tag="PW109_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2510830.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="PW109_000965"
     CDS             complement(2079..3053)
                     /locus_tag="PW109_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL2510831.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4304
                     /locus_tag="PW109_000966"
     CDS             3336..4304
                     /locus_tag="PW109_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2510832.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4401..4694
                     /locus_tag="PW109_000967"
     CDS             4401..4694
                     /locus_tag="PW109_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2510833.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="PW109_000968"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="PW109_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2510834.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5151..5906
                     /locus_tag="PW109_000969"
     CDS             5151..5906
                     /locus_tag="PW109_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2510835.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="PW109_000970"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="PW109_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2510836.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="PW109_000971"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="PW109_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2510837.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="PW109_000972"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="PW109_000972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2510838.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="PW109_000973"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="PW109_000973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2510839.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="PW109_000974"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="PW109_000974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2510840.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="PW109_000975"
     CDS             10643..11185
                     /locus_tag="PW109_000975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2510841.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11208..12764
                     /locus_tag="PW109_000976"
     CDS             11208..12764
                     /locus_tag="PW109_000976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2510842.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="PW109_000977"
     CDS             12778..13680
                     /locus_tag="PW109_000977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2510843.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEVSSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="PW109_000978"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="PW109_000978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2510844.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="PW109_000979"
     CDS             15123..15545
                     /locus_tag="PW109_000979"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2510845.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="PW109_000980"
     CDS             16014..16244
                     /locus_tag="PW109_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2510846.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="PW109_000981"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="PW109_000981"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2510847.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="PW109_000982"
     CDS             17655..17831
                     /locus_tag="PW109_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2510848.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="PW109_000983"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="PW109_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2510849.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18568..19035
                     /locus_tag="PW109_000984"
     CDS             18568..19035
                     /locus_tag="PW109_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510850.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            19120..20004
                     /locus_tag="PW109_000985"
     CDS             19120..20004
                     /locus_tag="PW109_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2510851.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            20118..20354
                     /locus_tag="PW109_000986"
     CDS             20118..20354
                     /locus_tag="PW109_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2510852.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20442..20522
                     /locus_tag="PW109_000987"
     tRNA            20442..20522
                     /locus_tag="PW109_000987"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20476..20478,aa:Tyr,seq:gta)
     gene            20529..20600
                     /locus_tag="PW109_000988"
     tRNA            20529..20600
                     /locus_tag="PW109_000988"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20561..20563,aa:Gln,seq:ttg)
     gene            20725..20895
                     /locus_tag="PW109_000989"
     CDS             20725..20895
                     /locus_tag="PW109_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510853.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            21115..21822
                     /locus_tag="PW109_000990"
     CDS             21115..21822
                     /locus_tag="PW109_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2510854.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21881..22525)
                     /locus_tag="PW109_000991"
     CDS             complement(21881..22525)
                     /locus_tag="PW109_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2510855.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            22651..22938
                     /locus_tag="PW109_000992"
     CDS             22651..22938
                     /locus_tag="PW109_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510856.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            23046..23366
                     /locus_tag="PW109_000993"
     CDS             23046..23366
                     /locus_tag="PW109_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2510857.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23447..24760
                     /locus_tag="PW109_000994"
     CDS             23447..24760
                     /locus_tag="PW109_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2510858.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            24757..25476
                     /locus_tag="PW109_000995"
     CDS             24757..25476
                     /locus_tag="PW109_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2510859.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            25604..26155
                     /gene="raiA"
                     /locus_tag="PW109_000996"
     CDS             25604..26155
                     /gene="raiA"
                     /locus_tag="PW109_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2510860.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26377..28911
                     /gene="secA"
                     /locus_tag="PW109_000997"
     CDS             26377..28911
                     /gene="secA"
                     /locus_tag="PW109_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2510861.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            29037..30138
                     /gene="prfB"
                     /locus_tag="PW109_000998"
     CDS             join(29037..29108,29110..30138)
                     /gene="prfB"
                     /locus_tag="PW109_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2510862.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            30243..30929
                     /gene="ftsE"
                     /locus_tag="PW109_000999"
     CDS             30243..30929
                     /gene="ftsE"
                     /locus_tag="PW109_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2510863.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30913..31806
                     /locus_tag="PW109_001000"
     CDS             30913..31806
                     /locus_tag="PW109_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510864.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGTKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
BASE COUNT        10668 a         5548 c         6898 g         8784 t
ORIGIN      
        1 gtttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatga gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat catccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaaaa catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaacc aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttgtggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaaa cgatcaaggt agtctgtacc tgcctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccacgactt attccttgag tttccacagc agtgaagctt
     2281 ttgaaacctt cacagatggc ctcctcgata aaataagcaa cagctaacgg atcattgatg
     2341 acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tgacagcagt attcgaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt gagattctcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggcgaacag
     2581 ttaccatggc ttttgtaagt tccacccatc gagacaaaac gttccatatg tttcccaata
     2641 tttgggttga ctttcaaagc agaagcgata ttggtcaaag gacccaaagc aatgactgat
     2701 gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag aggttctgcc
     2761 tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc atgagtgtct
     2821 tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac aggaatgtct
     2881 aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc tgcagggaca
     2941 ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac agctaatgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg cgaatgtaaa
     3181 tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt ggataataga
     3301 tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat ttcattctta
     3361 ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat cgtttgatgg
     3481 accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa gagtgtgaaa
     3541 gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg aaactagctg
     3601 gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta catgattacc
     3661 gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat gaacagccga
     3841 atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg atggcaagtg
     3901 gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat gtttctgttt
     3961 atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa atggtcatga
     4021 gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa gcatcagcta
     4081 cgagacagct tggcagcaat catctgcctt tattagtcat ccatggtgaa agagatcatt
     4141 ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat aaagcactgc
     4201 tgcttgttcc ggaagcagaa cacttggaag catcgatgaa agatccgaaa acctattgga
     4261 cagtgatttt ttcatttatt gaacaaagaa taacgatgaa ttaattttct cttctttttt
     4321 gtaagtaatt cgaggataaa taattgttta tagatcagga gactgctaaa ataaggtagg
     4381 aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga agaaaaaatc
     4441 gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg tcagatgcgg
     4501 gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt
     4561 tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg aaaaagatca
     4621 ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat caagacgtct
     4681 tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta ttccatttat
     4741 atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca tatttatctt
     5041 cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgagacgag
     5161 tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc
     5221 acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg gaaaaagcag
     5281 attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat tttttacagt
     5401 ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc tttgctgtac
     5461 gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg
     5521 taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg aagacagcag
     5581 gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta gctgaacaac
     5641 aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca gatatccaac
     5701 agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat ggcgtatttg
     5761 aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct gaaagtggtc
     5821 atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg ctgaaggttt
     6001 agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa aaccaatctt
     6121 gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg gagacaccgt
     6241 agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag aggggaaagt
     6301 agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg cttattcaga
     6361 agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata gaaagctcaa
     6421 ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa gtattgtgct
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg tcgtggctaa
     6601 tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag ttccagatca
     6661 aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca ttgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat tagataacgg
     6781 taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac aaggcagtca
     6841 attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc gagtaattcc
     6901 gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg ttccaaggct
     6961 gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc atgatatttt
     7021 cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt tccaagaaat
     7141 gggtgaattg caccgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga tttcttttga
     7201 ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca tccagctaag
     7261 aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca atgaaacggt
     7321 agctgaacat tataataaat taaatctgcc gtttatttac cgaatacatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac ggccaaaaac
     8101 acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc cagaaagaaa
     8161 tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg agatcaagtc
     8401 gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca acggagaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg aaaatgaatt
     8581 gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg tgtataaaga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg ttagcaattc
     9661 cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa gcatttgtct
     9721 tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa taatattcgt
     9781 tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt tgtcgccttg
    10021 attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt ggtttgttac
    10081 tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat caatcaatat
    10621 atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg gctatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg atcttttgtt
    10921 catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt tgggaagtgt
    10981 cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgt ggccaacgtg
    11701 aattagttat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagt ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac tggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtaa
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgttt cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgttgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttaa
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtatttc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg cgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaacaa tttggaaaat agcttcttat attttgcaaa
    18301 attgtgagtt attgccgaag tgttggattt tgaacaccgt caaaaaaaag gttgtgaaaa
    18361 atacgagtag cactgagcga taaagaaaca atttggaaaa tagcttctta tattttgcaa
    18421 aattgtgagt tattgccgaa gtgttggatt ttgaacaccg tcaaaaaaaa ggttgtgaaa
    18481 aatacggcta gcactgagcg ataaagaaaa agaggaaatt tattgtcaaa gtaacaactc
    18541 cgcaactact tatatggggg acaaaaaatg atcgaagaac taaacaagaa agatttacca
    18601 tggtccttgt tattagatgc agatcctgat aaagaaaaag ttcaagtata tgtttccaga
    18661 gggagtggct ggatatggaa agaaaaagaa aaaacaatag gtgtactgat ctatgtggca
    18721 agggaaaaag aatacgaaat cgtcaatgta gcagtaaccc caacgtatca aggaaagggg
    18781 attggcggaa aattacttga gacagctttt cagaagcttt cacaactcgc ttccagtcaa
    18841 actcagatca tcatacgaac cggtagtact agcagtgcgg cgttacatct ctatcaaaaa
    18901 atgggctatg tggagattgg tcgagtcaaa gactatttta tccatcatta ctctgagccc
    18961 atttttgaaa atggagatca attaagggat caagtgattt tagcaattca cttacctaaa
    19021 aatagcttta actaataaaa tttcaattca agaaaatgct ttcaaatgct aaatagatac
    19081 attacaatag ctttagcaaa caaaaaggag aatattttta tgggaatatt agtttttgat
    19141 tttggtggat cggctgtcaa atatggttgt tgggatggca aagagattaa aggtaaggga
    19201 caatttgcaa cgcctgaatc ttgggaagaa atgaaagcgc aattatttca agtttataaa
    19261 aaaatactcg tttcttttga agtcgaagga attggaatca gctctcctgg agtagtgaat
    19321 aatgagaaac aggtgattga gggaatcagt gcgattcctt atattcatgg atttaatatt
    19381 ttccgagatt tggaaactct gtttcaactt ccagtaacga ttgaaaatga cgccaactgt
    19441 gccggaatgg ctgaatttta tgaaggtgct gcccgcgatt atcaagatgt tgcttttgtt
    19501 gtggcaggaa ctgggattgg cggggcgttg ttcacgaaag gacaaatcaa taaaggggca
    19561 catctttacg gtggagaatt tgggttgatg ttcttaaatg gcgataagac attcagtaaa
    19621 cttggtacag cggtacagat ggcttggcgt tattgtgaac gcaaaggtct cgataaaaat
    19681 gcttataccg gaaaagatgt ctttgaatta gcagaaactg gagatgcaat agcaaaagaa
    19741 gaagttgaga gtttttatac gtatcttaca aagggattat tcagtattca attttcgttt
    19801 gatccagaag tgatcgtatt aggcggcggg gtttctgcaa aagaagggct cattgatgag
    19861 atcaaaagcc gaatgaagaa gctgacagaa caattcgatc tgcatgattt cgaaccgcag
    19921 attttattat gtgaatacag aaacgatgct aatcttgtag gtgcagcagc aaactatctc
    19981 gctgtacgtc aaggggagaa ttaagagctt tagagaaggt gcagcttgca taaaagaagt
    20041 gaatgaaatg aataggagat ttttacctaa ggaatgtttt atttgctgca gtatgataaa
    20101 atatctgaga ggtgaaaatg atgtcaaaaa aaaataaaga tattgaagtg cgtatagaag
    20161 aagtgaaaaa agatatcaaa ggtaagaatt atgaagtcac tcagttattt atagggaaaa
    20221 aaatgattgg tgagatctta gcctatggac caaaagaata tgaaattttc tttggtgaag
    20281 aagattttgg aaaagaaaaa agtcttgaaa atgcgataga aacagtgatt cgacactgga
    20341 atctgcacga ataaaaaaat atcaaaaaaa ctttcaataa agggttgcaa aaatctggaa
    20401 aattaggtat tataactaag tcaggttgat acaaaccttt cggaggagta gcgaagtggc
    20461 taaacgcgac ggactgtaaa tccgttcctt agggttcagt ggttcgaatc cactctcctc
    20521 catttcattg gggtatagcc aagcggtaag gcaagggact ttgactccct catgcgttgg
    20581 ttcgaatcca gctaccccag tataccacaa tcgtaagctc tcgttagttc gagagctctt
    20641 ttttttgctt tgctaccttg ttccttgaag attctgctga tttaagatag agttaagtta
    20701 actacaagaa ttggagggat ccacatgaaa tggaaagaaa aagtagacga agcagctgaa
    20761 aagttatatg atttggtcaa atcagaaaaa tacaatattg aagtgaatat tccgaaaaaa
    20821 ggcggaaaag cggtccgggt aaaatcaaaa cgcccaacga atcatacaaa aaaatggatg
    20881 gctaaaaacc gctaataata acaaaagatc cataagtcgg cattttcatc gattttaggg
    20941 tcttttgtta ttttctaggt tggacatatc taaaaaattg tttctgacaa taggagtagg
    21001 aagaacgatg tgtgtctaaa tcctctgttg atggatagaa acctttatct ttttcagttg
    21061 tgaatcaggt atataccagt tataatagaa gaaacgaaag gacgggtgta cgttatgtcg
    21121 aatcaattac ctgtatatat acagattcat gaccaattaa aaaatgaaat cgaacaaggt
    21181 atatggaaag tcggagaccg tcttccttca gaaagagaat tagcagtgaa attcaatgtc
    21241 agccgtatga cgttgagaca agcaattcaa aacttggcag atgaaggaat cttagaaaga
    21301 aagatcggtt cgggtacata tgttgcacga gaaaaagtac aagaaaaaat gtctggtgca
    21361 acaagtttta cagaaattat ggaatcacaa aatcgagtac cgtctagtcg gacaatctct
    21421 tatttcttga cttcgccaag ctcaagtgaa atggaaaaac taaacttgag taaagatgac
    21481 acgatcttgc gaatggaacg tattcgtttc gcagatgatg tccctatttg ttttgaagtt
    21541 gccagtgtgc cacaaaagct gatcgatggc tacagcaaag cagagattac tgaatcgttg
    21601 tatcgcacgc tagaagaaaa aggcgggaaa aagatcggtc cagcgaatca aacgattact
    21661 gctgtgttgg cttctgagaa aattgctgac tatttagagg taaaacgagg agatgcagtt
    21721 cttcgtttgc gccagatttc ttattttgaa gacggtacgc cgtttgagta tgtgcgtacg
    21781 caatacgtag gaaatcgttt tgagttttat ttggaaaaat aaaaaacagg tgatccctga
    21841 ttccgtatta gaaataaaag agaatcagaa tcacctgttt ttatatatga tcagttacca
    21901 gttgctcgtg ataagaaaca ggaccctctt caaattggac ttgtccgttc agcagctcaa
    21961 tgatggattc tttaaagtgg tcagcacttt tttcatctac agcacagatg acgctaactt
    22021 gatcagtata ttggatttct tttataaaaa aatgttggag ttcaagataa ttctgtaatt
    22081 ttcctagttg cggatacgtg atcatcacag agatctcctg ttgcaaggta ccttctacga
    22141 ttccaatgtc agacaatgct tgagagaccg catgagtgta tgctcgtatt aatccgcctg
    22201 tgccgagttt cgttcctcca aaatatcgag tgactactgc agctacattg atcagttgat
    22261 tttttttgag gacttccaac atcggtatgc ccgccgttcc acttggttct ccatcatcgc
    22321 tgcttcgctg gatttcattt ttttctccaa tgatgaaggc gctgcagtta tggttcgctt
    22381 tccaatgttc tttttttatt tcttgaatga aactttttgc ttcgtcctct gattctattc
    22441 gtctaagaga acagataaag cgtgattttt tgatctcgat ctcactttgt ccattttctt
    22501 ttattgtacg atagcgctcg atcatcttgt cacctctttt ttcattttct ttattcagta
    22561 taaataaagt tctaggttgt ttcaacactg taacggttgc gcaattgctg tagtatgcga
    22621 taataaagac aaaggcaggt ggatctgggg atgcaggaac gaatgaaaaa gtacgatgca
    22681 attacgcatt atttgaagaa caatggcggc tctcaagtaa cgttgacctt tacccaattt
    22741 gacgaactgc tgtttcccag taacggactt cccaaaacag caagggaaag tacagattgg
    22801 tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg gctggataaa tgcaggctat
    22861 gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt tcaataaatt agtgaaatct
    22921 agttggctat ttgattaaat aaaccgcgtt tagaaaacgt tgacaaattt ctttcttttt
    22981 atataataga gtaacagacg cgaagaaaac gtttgcgtag tttgttttaa aaaggagaga
    23041 aaagtatggc taaaaaaaca atcatgcttg tatgttcggc tggtatgagc acaagtttac
    23101 tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat cgattctgat attttcgctg
    23161 tgtccgcttc tgacgcagat aacaatcttg caaataaaga cgtggatgtg ttgttattag
    23221 gcccacaagt tcgctttatg aaagctgatt ttgagaaacg tctggaacca aaaggcattc
    23281 ctttagatgt catcaatatg gctgattatg gtatgatgaa tggcgaaaaa gtgttagatc
    23341 aagcaatcac gctcataggt gaataaatag aacagtataa ctatcgaaca agcgatccaa
    23401 aaaatagatc gcttgttttt tcgtatattt ttttagggcg aataatatga atcatttttg
    23461 gggtagaagg cttctgacga gagatattaa agcaatagac tgtgaggagg taaatttacc
    23521 aaattatgag aagatcgcag cagtggaacg tttgagcaac tggattcttt gtcatcgctg
    23581 tggaatgaga acgtccgtca ttgatggaca gcttgcagat tacggttatt tttgtacaca
    23641 ttgtctatat ttagggcgat gcgacactca acaagattta tatttgtttg atcagcctga
    23701 agcaaaacct cgagaagtcg tctttttttg gacaggacag cttactgaaa aacaaacgga
    23761 aatctcaaag agaatacttt gccattcaga aaaaagacat catcttattt gggctgtaac
    23821 aggtgcaggg aaaacagaga tgctttatcc tattttagtg gaaactttaa aagctggagg
    23881 aagagtagcg atttgtacac cgcgagtcga tgtttgtaat gaattgtttt tacgctatcg
    23941 ttatgttttt ccagaagaaa aaatcacgtt gctccatggg aatacagcaa cggcttatac
    24001 ctttacttct tttgttatct gtacgattca tcagctatac cgtttttatc ggtcctttga
    24061 tttaatagtg atcgatgaga tcgatgcgtt tccttataac ggggatgcag gattagctca
    24121 tgctgtgcaa acagcgatta aaccagacgg acgattcatc tacttaagtg ccacacctga
    24181 taaaaagtta ctaaaggaga tcaagcagac gtttgacatc cacaaacttg ctttacgctt
    24241 tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg aataattggc gacaaaaatg
    24301 caagaacaag aagaagataa aatctttggt gcggatcatt catcaattaa ttaaagaaaa
    24361 ccatatcctt ttattttgtc caagtatcac gatgatgcat cgtttgaaag ccacacttca
    24421 agaggctctt ccgagatatg agatcacaga tgtttcttca agagacaatc agcgggcgga
    24481 aaaagtccag aaaatgcgag aagcaaaata tgacatactg ttaaccacga tgatccttga
    24541 acgtggagtc acatttgaaa gaatatccgt tatcgtttta ggagcggatc atccggtttt
    24601 tactaaatct tctttggtac aaatcgcagg aagagccgat cgtaaagggc cttatacgaa
    24661 cagtcaagta tattttttct atgaagaaaa aactgcagct attcgaaaag cttgtcagga
    24721 aatcaaagaa atgaatgaag aagggaagaa gatactgtga ggtgcagttg ctgcagtaaa
    24781 gatatcgtcc aaaatttaac attcagcgag attattggtg tttcgaaata cagattacct
    24841 cagacttgtt cagtttgtcg cgcacaattt catttattgg aaacaagagg ctgttctggc
    24901 tgtcaaaaac caatccattt caatctagag agtacgaaaa agaaagtaaa ttctatcccc
    24961 gtttactgca gtgactgtca agaatggaag aagaaatatc cgacctatga ttttcagcac
    25021 cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc tcaatcaata caagtttatg
    25081 ggagatatcc gactagcagg cacctttgca tataaatgga cgcagataaa aaaagaatac
    25141 ccgacataca tctattgtcc aataccttta tctgaaaaaa ggcgaacaga aagaggattc
    25201 aatcaagtaa gcgagatgtt gaaagcagca agaattcctt ttcagatgct actcaaaaga
    25261 gagaagcatc tgctggctca agcaaaaaac acgcgaaagg aaagattgga gatgcctcaa
    25321 ccttttgaac ttgcagtcac cttttctaag atacaaaata aaaaaatatt gattattgat
    25381 gatgtctata caacaggaca gactcttttc cacgcagcag attgtttgca gatcgcacag
    25441 ccgaaaatca tccgaactat ttctttggcg agatgaatta gatgtggaaa tttgatagcg
    25501 atttaatttg caaataacct agagttcgat ataatagaat taagaagagg gaaagagtgg
    25561 tccttctcct tcttgattgt ggtagacgaa aggggtaatt tttatgttta gatataatgt
    25621 acgcggcgaa aatatcgaag taactgaagc gattcgcgac tacgttgaga aaaaagtagg
    25681 caaactagaa cgctatttca gtgacgcacc cgatgcaacg gcgcatgtga acttaaaagt
    25741 ttatacagaa aaaacagcaa aagtggaagt aacgatccca ttaccttatc ttgtattacg
    25801 tgccgaagaa acttctccag atttatacgc aagtatcgat ttggttgttg acaaattgga
    25861 acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa tcccgtgaaa cgggcatgaa
    25921 tactgcaaat gctgctgctt tattcaatga agaggacgaa aacgaaaatg attctgaatt
    25981 agatatcgta cgtacgaaac gcctttcatt gaaaccaatg gatagtgaag aagcagtctt
    26041 gcaaatgaac atgctgggac ataacttctt tatttttgaa gatgcagaaa caaatggtac
    26101 aagtatcgtt taccgccgta aagatggaaa atacggactg attgaaacag actgatcagc
    26161 cttacttaaa taacaacgaa aacttgcagc agctactgac agctgccgca agtttttttg
    26221 agtttcctcc tcttttagat gaaatatgaa taaattatga agttcttact ttaatacgct
    26281 taagatattt caattatcta taactaatga taaaatgaaa agtaaagtct acatagtctg
    26341 gataatacac agaaggaaaa ggagattaca caacgcatgg ccaatttgat tcgttcactt
    26401 atcgaaaacg ataagaaaga actaaaacgt ttagatagca ttgcaaaaaa agttgaagca
    26461 tacgctgacc aaatggcagc attaactgat gaacaattac aggcaaaaac tgctgaattc
    26521 aagggcagat accaaaaagg agaaacatta gatcaattac ttccggaagc ttttgcagtc
    26581 gtccgagaag cagcaaaacg tgtattaggt ttatatccat accacgtaca attgatgggt
    26641 ggaatcgtac ttcacgatgg aaatatccct gaaatgcgta ctggtgaagg taaaaccttg
    26701 accgcaacga tgccagtata tctaaatgct ctgactggcg aaggggtaca cgtagttacc
    26761 gtcaatgaat atttagcaac ccgtgactcg acggaaatgg gcgaacttta taatttctta
    26821 ggattaacag tggggttgaa tatcaactca aaatcttcgg atgaaaaacg cgaggcttat
    26881 aattgtgata tcacttatag tacgaataat gaattaggat ttgactactt gagagacaat
    26941 atggtcgttt accgccatca aatggtacaa cgtccgctga attatgccat tgtcgatgaa
    27001 gttgactcaa tcttgattga tgaagcgaga acgccgctga tcatttcagg gcaagcagaa
    27061 aaatccacag ctttatatac aagagcagac aacttcgtga aacgtttgaa agaagaagaa
    27121 gattataaaa tcgatgtaca atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa
    27181 gcagaagaaa cattcggttt ggataactta tacgacattg aaaatactgc attgacccat
    27241 cacttggatc aagcattgag agccaactat atcatgcttt tagatatcga ttatgttgtc
    27301 caagacggaa aagtactgat tgttgaccag ttcactggtc ggatcatgga tggacgccgt
    27361 tattcagatg gacttcacca agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa
    27421 acgaaaacca tggcgacgat cactttccag aactatttcc gtatgtacaa gaaacttgcg
    27481 ggaatgactg gtacagctaa aacagaagaa gaagaattca gagaaattta taacatccaa
    27541 gtataccaga ttccgacaaa tcgtccggtt atccgtgatg atcgtccaga tttgctttac
    27601 ccaacattac aaagcaagtt ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa
    27661 ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat ctgagttatt atcagatatg
    27721 ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga aaaatcactt taaagaagca
    27781 gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga ttgctaccaa catggctggt
    27841 cggggaacag atatcaagtt aggacttggg gtaagagaac ttggtggtct agcagttatc
    27901 gggacagaac gacatgaatc tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt
    27961 caaggagacc ctggtatgtc acagttctac ttgtcattgg aagatgattt gatgaaacga
    28021 ttcggttcag aacgaatcaa agctttctta gatcggatga agatcggtga ggaagatgct
    28081 gttatccaaa gcaagatgct gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga
    28141 aataactatg acacacgtaa aaacgtctta caatacgatg atgtgatgcg tgaacaacgt
    28201 gaagtaatct ataaacagcg acaagaagta atcatggaag aaaaaagctt atccaagcca
    28261 ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag atgcgcatac acaattagag
    28321 aaagaaaatt ggaatttgga aagtatcatc gattttgccg gcagtactct tgtccatgaa
    28381 gatagtatta ctcttgcaga tatcgaagga aaaacagctc aggagatcaa agagtatcta
    28441 aacgaacgag cacaagaagt atttaatacc aaagcggctc aactaaatgg tccagagcaa
    28501 ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg actctaaatg gacagaccat
    28561 attgacgcaa tggatcaatt acgtcagtca atcggcttac gtgcttatgg acagaacaat
    28621 ccattagtcg aatatcaaac agaaggctat aagatgtttg aagatatggt aggcgccatc
    28681 gagtatgaag tcactcgaat ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa
    28741 caggtttctc aaggaacagc ttctcactca gaagacggag atgccaaaga aaatagtaat
    28801 actagcgcga aaaaacagcc aatccatgtg aacaaggtcg gaagaaatga tccttgtcct
    28861 tgtggaagcg ggaaaaaata taagaactgt catggaaaaa atgcaaacta atttctgaca
    28921 gcaggattgg tgagacaaat tcttttttta agaattcgtc tcaccttctt tctgttattt
    28981 gctgattctg ttagactaga tataatgaaa aaagtatagt gaaagaagga aaaaacatgg
    29041 aaatatccga aattcgaaat ctcctgacag aaatgcagga aaaggtcaca agtttcagga
    29101 ggtctctttg acttagatca gctggaagaa gacatcgcgg aagcagaagc aaaaatggct
    29161 gaacctggtt tttgggatga cagcgaagcc gcacaaaaag tgatcagtga aaataacgct
    29221 aataaagaaa cctatgacca attccattct cttgccgaag aactagaaga aatcgaattg
    29281 atggtggaga tgctgcaaga agaacctgat gcagcaatgc aagaagaagc agaaacacgt
    29341 atcaatgcat tagatgaaaa aatgaagacg tatgaattat ctatgctgtt agacggacct
    29401 tatgatcgaa acaatgcgat cgtcgaattg catccagggg ctggcggaac ggagtcccaa
    29461 gactggggca gcatgctttt acgtatgtat acacgttggg cagagcagca tggctatcaa
    29521 gtagaaacac tagattatca agcaggtgat gaagcaggga tcaagagtgt gaccctgctg
    29581 attaaaggac ataacgcttt tggttatcta aaatcagaaa aaggtgttca tcgccttgta
    29641 cggatttcgc cttttgactc agcgaaacga cgtcatactt ctttttgttc ggtagatgtg
    29701 atgccagagc ttgatgaaac gatcgatata gaaatcaatc cagatgattt gaaaatcgat
    29761 acgtaccgag caagtggtgc aggtggtcag cacatcaata aaacagaatc tgctgtacgg
    29821 atcactcact tgcctacagg aacagtagta gctagtcagg cacaacgctc acaattgaaa
    29881 aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt atcaattaga agtggaaaag
    29941 aaagaacaag aagcagctgc acttcgagga gaacaaaaag aaatcggctg gggttcacag
    30001 atccgttctt atgttttcca tccctattct atggtaaaag accatcggac aaacgtagag
    30061 acaggaaatg tccaagcagt tatggacggt gaccttgatt tatttattga cggctattta
    30121 aaaatgcatc tacaataaga acccattgtt aaattgaaat aaaattgtaa gattttgcaa
    30181 gcatgttgaa acattgccat gctataatag ctaaagactg aaaaagatat ggagagaacg
    30241 ccatgattga aatgaaggat gtaatgaaga agtactcaaa tggtacgacc gctatccgca
    30301 atctctcgat tgtcatcgaa caaggagagt ttgtctatgt ggttggtccg tctggagccg
    30361 gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga
    30421 acgtcgcagg tcatgatcta atagcaatca aaaaccgcga agttccttat ctacgcagag
    30481 aaatcggcgt ggtgttccaa gactataaat tattaccaag aaaaacagta tatgaaaatg
    30541 ttgcgtacgc tatgcaagtt atcggcagaa aacctcgaga tatcaaaaaa cgagtaatgg
    30601 aagtgctgga ccttgttggt ttgaaacaca aagttcgagt ctttcctagc gaattatccg
    30661 gtggggaaca gcaacgtgtc tctattgctc gggcaatcgt gaatacgcct aaagtactta
    30721 tcgcggatga accgacagga aatctggatc cagaaaactc atgggaaatc atgaaattat
    30781 tagatcggat caatgcccaa ggaactactg tcgtgatggc tacacataac agtacgatcg
    30841 taaatacgat tcggcaccga gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag
    30901 cggaagggga atatggatac gatgattaga acctttttcc gccatttatt agaaagtatc
    30961 aagagtttga aacgtaacgg ctggatgaca gttgcttcag tcagtgcggt aacgatcacg
    31021 ttgactttag tcggtgtatt catggcagtt atcatgaatg caactaaatt agctcaggat
    31081 atagaaggaa acgttgatgt atctgtattc gtagatatcg gtacaaaaga cgaagagatg
    31141 aaaaagctag aaaaagaatt gaatgactta gatcatgtta aaaaagttga attttcaagt
    31201 aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg attcctggaa attgtttgaa
    31261 ggtgacaata acccgttata tgatgtgtat gtagtaagtg ctactgatcc ttcttataca
    31321 aaacaagtag cagaagaagc agcagatttg aagaatgttt ctcgttctga ttacggtgga
    31381 gcttcctcag atcggatttt ccaaatcgct ggtgcagttc ggacatgggg attagttgca
    31441 gcggcattgc tgctgttcgt agcgatgttt ctcatttcga acacgattcg gatcacgatt
    31501 ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg gtacaaaaaa tggatatatt
    31561 cgctggccgt ttttccttga aggtggctgg atcggtctga tcggtgcgat tcttccgatt
    31621 ttgatcatca catttggtta tgcatggttt ttccaattga tcaatccatc attattacgt
    31681 tcccattatt cattgattca tccacaaaat atcgtttgga aaatcaatct tttaatggtc
    31741 ggcattggtg tgatcattgg ttcacttggc tcgatcattt ctatgagacg tttcttgaaa
    31801 ttctaatacc acaatataaa taaaatgaga aagttggaca gtttcgttcc agctttcttt
    31861 ttttattggc taggagaata gaaggaagcg gctttttt
//
LOCUS       DANKHK010000024        31603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000024 DANKHK010000000
VERSION     DANKHK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            51..1100
                     /locus_tag="PW109_001001"
     CDS             51..1100
                     /locus_tag="PW109_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2510865.1"
                     /translation="MDKIFKIFVSSTFTDLEDVRLQAMQGVLKAGHMPIMMEGFAATA
                     TQKEIIKKKITSCDAYMLIVGSKYGTIDPESGLSYTEWEYDYALDNGIPIYTMILEEK
                     YIQERMANGLLKLSDIETTSENYKAFVSKASSRQVDFINSIEQVKSMTEAGIMQLVKD
                     FGSSMIGWVSATIVDNVKMLENELDNLRKTRSELQVKLTNAETREIRESELPPNSSFD
                     GLLSKMFAPNSTDPILEQGMIYIMEFIREKVEQLKATIESHRGNIALGYDANKKDQVL
                     LRLGKDCLFFLADYNKAIINVRFAIQDPYFSENIDTLHVKNDVLYSEKYSEPLSLDYL
                     NKTLSQYHEIKKQQN"
     gene            complement(1357..2502)
                     /locus_tag="PW109_001002"
     CDS             complement(1357..2502)
                     /locus_tag="PW109_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2510866.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(2563..3210)
                     /locus_tag="PW109_001003"
     CDS             complement(2563..3210)
                     /locus_tag="PW109_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2510867.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            3404..3697
                     /locus_tag="PW109_001004"
     CDS             3404..3697
                     /locus_tag="PW109_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510868.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            3741..4052
                     /locus_tag="PW109_001005"
     CDS             3741..4052
                     /locus_tag="PW109_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510869.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            4049..4673
                     /locus_tag="PW109_001006"
                     /pseudo
     CDS             4049..4673
                     /locus_tag="PW109_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            4814..4972
                     /locus_tag="PW109_001007"
     CDS             4814..4972
                     /locus_tag="PW109_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510870.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            4973..5335
                     /locus_tag="PW109_001008"
     CDS             4973..5335
                     /locus_tag="PW109_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510871.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            5372..6220
                     /locus_tag="PW109_001009"
     CDS             5372..6220
                     /locus_tag="PW109_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2510872.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            6210..7685
                     /locus_tag="PW109_001010"
     CDS             6210..7685
                     /locus_tag="PW109_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2510873.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            7951..8358
                     /locus_tag="PW109_001011"
     CDS             7951..8358
                     /locus_tag="PW109_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2510874.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            8361..8576
                     /locus_tag="PW109_001012"
     CDS             8361..8576
                     /locus_tag="PW109_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510875.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            8580..8960
                     /locus_tag="PW109_001013"
     CDS             8580..8960
                     /locus_tag="PW109_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2510876.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            9094..9249
                     /locus_tag="PW109_001014"
     CDS             9094..9249
                     /locus_tag="PW109_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2510877.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            9318..9791
                     /locus_tag="PW109_001015"
     CDS             9318..9791
                     /locus_tag="PW109_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2510878.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            9788..11482
                     /locus_tag="PW109_001016"
     CDS             9788..11482
                     /locus_tag="PW109_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2510879.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            11448..11633
                     /locus_tag="PW109_001017"
     CDS             11448..11633
                     /locus_tag="PW109_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510880.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            11637..12812
                     /locus_tag="PW109_001018"
     CDS             11637..12812
                     /locus_tag="PW109_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2510881.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            12805..14328
                     /locus_tag="PW109_001019"
     CDS             12805..14328
                     /locus_tag="PW109_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2510882.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            14384..14668
                     /locus_tag="PW109_001020"
     CDS             14384..14668
                     /locus_tag="PW109_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2510883.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            14655..14990
                     /locus_tag="PW109_001021"
     CDS             14655..14990
                     /locus_tag="PW109_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2510884.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            15285..15911
                     /locus_tag="PW109_001022"
     CDS             15285..15911
                     /locus_tag="PW109_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510885.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            16653..17489
                     /locus_tag="PW109_001023"
     CDS             16653..17489
                     /locus_tag="PW109_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2510886.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            17554..17985
                     /locus_tag="PW109_001024"
     CDS             17554..17985
                     /locus_tag="PW109_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510887.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(18053..19375)
                     /locus_tag="PW109_001025"
     CDS             complement(18053..19375)
                     /locus_tag="PW109_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2510888.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(19529..21169)
                     /locus_tag="PW109_001026"
     CDS             complement(19529..21169)
                     /locus_tag="PW109_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2510889.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            21499..21969
                     /locus_tag="PW109_001027"
     CDS             21499..21969
                     /locus_tag="PW109_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2510890.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            22598..23701
                     /locus_tag="PW109_001028"
     CDS             22598..23701
                     /locus_tag="PW109_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2510891.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            23985..24665
                     /locus_tag="PW109_001029"
     CDS             23985..24665
                     /locus_tag="PW109_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2510892.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24755..25789
                     /locus_tag="PW109_001030"
     CDS             24755..25789
                     /locus_tag="PW109_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2510893.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25803..26138
                     /locus_tag="PW109_001031"
     CDS             25803..26138
                     /locus_tag="PW109_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510894.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            26122..28602
                     /locus_tag="PW109_001032"
     CDS             26122..28602
                     /locus_tag="PW109_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510895.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGDFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28658..30217
                     /locus_tag="PW109_001033"
     CDS             28658..30217
                     /locus_tag="PW109_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510896.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            30214..31578
                     /locus_tag="PW109_001034"
     CDS             30214..31578
                     /locus_tag="PW109_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2510897.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT        10660 a         5331 c         6431 g         9181 t
ORIGIN      
        1 atgttaagta taaaattaat gactttttgg tctgaaagct gagggattca atggataaaa
       61 tattcaaaat ttttgtgtca tcaacattta ctgatttaga agatgttcga cttcaagcaa
      121 tgcaaggggt attaaaagcg ggacacatgc ctataatgat ggaagggttc gctgccacag
      181 ctacacagaa ggaaattata aaaaaaaaaa ttactagttg tgatgcgtat atgcttattg
      241 ttggtagtaa gtatggaaca atcgatccag aatcagggtt gagttataca gaatgggagt
      301 atgattatgc attagataat ggtattccaa tatacacgat gattttagaa gaaaaatata
      361 tacaggaacg tatggcaaat gggttactta aattatcgga tatagagact acttctgaaa
      421 attacaaagc ttttgtgagt aaagctagca gtaggcaggt tgattttatt aattccatag
      481 agcaggtaaa aagtatgaca gaggccggaa tcatgcagtt ggtcaaggac tttggaagct
      541 cgatgattgg ttgggttagc gcaacgattg ttgataatgt taaaatgtta gaaaatgaac
      601 tagataattt gagaaagaca agaagcgagt tacaagtgaa attgacaaat gcagaaacaa
      661 gagaaataag agagtcagaa ttaccgccaa atagtagttt tgatggatta ttatcaaaaa
      721 tgtttgctcc taactccact gaccctatcc ttgaacaagg aatgatttat attatggagt
      781 ttattcgaga aaaggttgaa cagcttaaag caacaattga gagccatcgt gggaatattg
      841 cattaggata cgatgctaat aaaaaagatc aagtactttt gcgcttagga aaggattgcc
      901 tatttttttt agctgattat aacaaagcta ttatcaatgt acgatttgct attcaagacc
      961 cttatttttc ggaaaatatt gatactctcc atgttaagaa tgatgtattg tacagtgaga
     1021 aatattcaga accactttca ctggattacc taaacaaaac gcttagtcaa tatcacgaaa
     1081 taaagaaaca acagaattaa atttcctgcc aaaagcacgc cacgcaattt gaatacgatt
     1141 ttgaatacga ttagaaaact aatcaagatg atttatcatt aataaagcga ttagctttaa
     1201 tgaaaaatca ttgaaatatc aactatcttc aattatcatc aatgaactta acttcatggc
     1261 ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg gtttctcctt
     1321 tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta agtgttgtac
     1381 taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac aatcgatcgt
     1441 ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag ctccagcgtc
     1501 caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg gaaccatagc
     1561 tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta gttctttata
     1621 gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat atttccgctg
     1681 attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa gaaccccagc
     1741 actacaatca gttttaggat cactgatttt atagccattt ttggattggc ttagtgtttt
     1801 attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg caagggcttc
     1861 actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa agtcgtttcg
     1921 ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt ctttactgta
     1981 aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata cgttgtctag
     2041 tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct tagaagttga
     2101 atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt gttgggcttt
     2161 ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc actttctaaa
     2221 cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg tttctagcca
     2281 caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt ttttatcgta
     2341 gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt aacctcgtct
     2401 acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt catagagcca
     2461 aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt tccctccata
     2521 gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg aatagtgttt
     2581 atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat aaaattcttg
     2641 atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt catatcgtaa
     2701 aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag gaaattcaat
     2761 tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct gtgagacagt
     2821 caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa ttatagagtt
     2881 atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa tttcgggcat
     2941 catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg atacatctaa
     3001 tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat tttcccaatt
     3061 actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt ccattgagag
     3121 tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt cggggtattt
     3181 ttttctagcc acattttcac caccattcat atattttctc tactatatca cgatttaaat
     3241 atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg aaaaggtatg
     3301 atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt gcattttttt
     3361 aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat taaaaattga
     3421 caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg ttcaacaagt
     3481 agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt ttccaaaata
     3541 tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta tgatgcagaa
     3601 atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa tttcacaaga
     3661 tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg gggcaaaggg
     3721 attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa aaagtattat
     3781 caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc aaagcgctag
     3841 aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta ccacaagaac
     3901 caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag tttaaagaac
     3961 gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg ttagcgagct
     4021 tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg gacacattat
     4081 aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg cggaagcgtt
     4141 aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg ttactactag
     4201 taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg aaaaaaaata
     4261 tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa gactggattt
     4321 tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa gtattccgca
     4381 tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct attactgaac
     4441 caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta tcaactattt
     4501 atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg gttgatcgtg
     4561 aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat gggttgcagt
     4621 tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact taggagcatt
     4681 aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga caggataaag
     4741 gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata ttaaaaaatc
     4801 ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg gcggaatttt
     4861 cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt tatatctctt
     4921 cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt agatgtacaa
     4981 aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc aagggatcgc
     5041 tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg aaaaactaac
     5101 agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag aaatcaacga
     5161 gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag aagcaagggt
     5221 gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg aatttgttcg
     5281 ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg aatgatttat
     5341 accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag cagcaacgga
     5401 attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa ttccaataaa
     5461 aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg aatggtggac
     5521 ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg tggtagttga
     5581 cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac ttgcttcaat
     5641 gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa tccatttctt
     5701 ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg ctgatatctt
     5761 cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta aaggagttcc
     5821 aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac ctattgaaaa
     5881 cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac tgctaaaaaa
     5941 accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag caaaaaaatt
     6001 agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa ctaagttatg
     6061 cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga tttatacagc
     6121 aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta ttcgatcgat
     6181 gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta gatagtattt
     6241 tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa acctggatgt
     6301 cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg aatgcagaat
     6361 tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa tttagcggat
     6421 acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa tgggaagaca
     6481 gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt tttgatgaca
     6541 acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac ccagtaaaag
     6601 aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg tttattgacc
     6661 ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg atagtcggat
     6721 cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt ttggatggac
     6781 cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat tttttcaccg
     6841 attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa ggcaatgtaa
     6901 ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag ataaaaaact
     6961 tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc atagcttggg
     7021 caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa gatgatacag
     7081 gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat ccgtttaaaa
     7141 caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt gataaaggac
     7201 aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca aaagactatc
     7261 aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat ttggaaatgg
     7321 aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat tatcagaact
     7381 atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga gctactatgc
     7441 aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa gctgtttttg
     7501 ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa aagattgcct
     7561 tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga aataaacgac
     7621 gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga aagtacaaaa
     7681 agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc cttactgccc
     7741 caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac ttttgaaata
     7801 gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg ttaccaaaac
     7861 gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac gtaggtgtta
     7921 ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac aaacaaagca
     7981 ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct ggcatttagt
     8041 gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa gataatggaa
     8101 gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt agtgggatca
     8161 ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt taattgtctg
     8221 cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga taatttgttt
     8281 ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt gataactaat
     8341 ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat ggtgagaaac
     8401 acattgagta ttcaaacact caagaagatg tagactttat tagttttgag gaagataaaa
     8461 tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct tatattgaat
     8521 acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt tggtaatcta
     8581 tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat tataggcaga
     8641 agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa agaaagcaaa
     8701 gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct tcatacttgt
     8761 atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata aggaaagctg
     8821 atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta gatgaaacta
     8881 attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa agacaaagac
     8941 gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga caatcgatgc
     9001 ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta caaataacga
     9061 ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt attgaaaaat
     9121 acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc tcattttggt
     9181 caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt agataaagaa
     9241 aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct ttttcttttg
     9301 tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa ggcaatatat
     9361 caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag cagcaaccat
     9421 taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa tggaatcgta
     9481 tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt tcattggtag
     9541 cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga aaatcaggta
     9601 cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg gttcaatcac
     9661 aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta gaatcacgat
     9721 caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc aaagagttga
     9781 tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg cacccaagaa
     9841 aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt cagatagctt
     9901 cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg agttgctacc
     9961 atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc taggcagtct
    10021 ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt ttaccagtat
    10081 gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg cgttgattat
    10141 ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt tgaaacaagc
    10201 taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt ctagtaagtt
    10261 tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct ctaattcgtt
    10321 cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa caaccgcaat
    10381 tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta agtcaggaat
    10441 gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg atttaaacgt
    10501 tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag aacaagccga
    10561 caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg atcaagagaa
    10621 atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa tgattccaac
    10681 cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg tattggtgaa
    10741 aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg aagactggca
    10801 ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg gagtggattt
    10861 atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata acggcgaact
    10921 ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca aagaaaagcg
    10981 cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta ctcaattaga
    11041 aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga ttcaagaaaa
    11101 tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc ttatcagtaa
    11161 agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta ctctaaacgt
    11221 tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca ataaaaatac
    11281 gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg gcattcagat
    11341 taataaatca aagaacagta acaaaattga tccaatagct gcattaatca atgcctatgt
    11401 gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg aattttatac
    11461 aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt ggattggtgt
    11521 gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta gcgctgggca
    11581 tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt taaaagatgg
    11641 cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat gtggtatcaa
    11701 tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga aatagtgatg
    11761 tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt caattggtca
    11821 aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag aaacctaatt
    11881 cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg ctaaacggta
    11941 atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac ttattaccta
    12001 actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag attggtgata
    12061 aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc caagatggtt
    12121 taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa caagctggca
    12181 accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt ttgaaggttc
    12241 ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt gaagaagcca
    12301 acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa acaatggact
    12361 acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat gattggaaca
    12421 caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt gaaaatgaac
    12481 attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt cattacttta
    12541 atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt aattccgatc
    12601 agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag gtcaaagggt
    12661 cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg gatggtggcg
    12721 atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa tatcaattaa
    12781 aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa acggctgaca
    12841 gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga aaaacaacca
    12901 acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc aaaagattta
    12961 ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt atcaaatgtg
    13021 ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc aggtacgttg
    13081 aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga cacgacctat
    13141 tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc ctttgggttc
    13201 gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg atcaatagat
    13261 aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga ttcatcaaat
    13321 gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc aaaacaaaca
    13381 aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat ggaaaaaact
    13441 ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg ttcacaaggc
    13501 gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga agaagtaatc
    13561 ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc aacagtaaaa
    13621 acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc agtgttagca
    13681 acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac ttcagttgat
    13741 tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt ggaagattca
    13801 gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga aaacactgac
    13861 aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc tacgttggac
    13921 gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat ggtaattcta
    13981 aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg ttacatttta
    14041 caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt agtattgatt
    14101 gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat gggggatatt
    14161 gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga aaaattcgat
    14221 tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat tgatcaaaat
    14281 gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa aaatattggg
    14341 cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa ttttggatag
    14401 cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc aacaattgat
    14461 tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg ataaagatat
    14521 ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt ggtatttgaa
    14581 ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag tacaacaaat
    14641 gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag agagtcaagt
    14701 ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt gaagaaactg
    14761 tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt gctagtgtag
    14821 gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat tatgaactaa
    14881 caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca atcaatgaag
    14941 gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag aatgattaca
    15001 cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt tagctgcctg
    15061 aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt aggtgtggtt
    15121 gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta atacaggcat
    15181 gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag aacacgtctg
    15241 ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac aaagcagagt
    15301 ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt aataccacag
    15361 attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat caaaaactac
    15421 tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga tttgatagca
    15481 gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa ttaatgtgtt
    15541 tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa ccttttggag
    15601 atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta aaacatggtc
    15661 gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta gcaagtctat
    15721 atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta gactttataa
    15781 gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg atgcaaaaag
    15841 tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt aaagaacaag
    15901 aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt gtatgccatt
    15961 tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt cataaacgct
    16021 gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg aagaagaaga
    16081 atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa acgtcttgtc
    16141 acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat tcgctcgaaa
    16201 aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag gtcaaaacgt
    16261 tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
    16321 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctgaa
    16381 cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg cggtaataag
    16441 ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    16501 aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa aagtagaaaa
    16561 gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt aacaatcgct
    16621 aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat tttgtggtga
    16681 tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg ttgatcaaac
    16741 cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac tcaacagttg
    16801 cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg aaatcactga
    16861 attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta gtcggcaaca
    16921 ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag ctgatccaaa
    16981 tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat catcagccaa
    17041 aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg ggctttttag
    17101 ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg ctgatgtttg
    17161 cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt tctcaactgg
    17221 caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta tctatgttgt
    17281 tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt agcagggttt
    17341 tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt cttttggtca
    17401 attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta acgcaagtca
    17461 aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata aaaaaattca
    17521 ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg tttagaagat
    17581 gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc attaaccgtt
    17641 acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt cttggtttat
    17701 gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta tgaatcgatt
    17761 tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc tgaaaaaaga
    17821 ggcatcggaa aggctttgat gactggcgta gaagaagcga aaaaacgagg aataacgaca
    17881 ataaggctta attctgcaga atacagagta gaagcacacc aattttatga acacatcggt
    17941 tatcattccg ataaaatgca aaaaagattt ttgaaaatcc tgtaaatctt tatcaaaaaa
    18001 atacccgatc accaagccga tacttgatga tcaggtattt ttgttttttt gactaagaaa
    18061 gtaattgcat caagaaacca gagatgatca aaacgatggc accaccaagt cgattgccca
    18121 tttgagcaaa agcaataagt tccatccgat tagctgcaga taagacggct acatttcctg
    18181 ttccgcccat cgagttgttg cataagccag cagtaatagt tgattcaatt ggatataaac
    18241 caaacaaacg accgacaaat cctgaaacaa ctgaaatcgc gataacactt gtcagacata
    18301 agacaacaaa ttgccatgtc aaagcagaag ctaatacatt cagattcagc aacgcaatac
    18361 cgataccggc aagaacagct gctgtcaagt ttttgacaat caagttattg aacatgatag
    18421 ctgcatcttc ataatactct ggtaataggt tggcgatttt acagatgaca acgataataa
    18481 tcataaatgc gtaagcatgg actttaggaa caaaatagtt accaatttgt cctaagatga
    18541 agaaagtcaa tgataccatc aaaccaacac ctaactgtgg aatggaaggt ttcacagttc
    18601 gaggttttcc atcgccaaga tcgccttttt ccattaattt accattgccg ttgaattttg
    18661 gcattgattg tccagcacga gcaactaatg ctgcaccgat gatagctaag acatttgtca
    18721 ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc atccgtacct aaaacatttg
    18781 cataaatcgt tgataaagga acggctccgg ctccaacacc accagccatc gcaggtaatg
    18841 agatgtataa aacggaattt gcaaaaccat ttccgataag catgcccacg agtcctacgg
    18901 caaaaaagga gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca
    18961 tcaatagatt acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt
    19021 ccaaaaagcc catattgctg acaaagtttt ctgtggattt cactacatat tcaggtaaga
    19081 tcccaaatgt agcaatcgca gcagaagcga agatcgcaaa aacggaacca ccacccaaat
    19141 aacttttgac gatagggatt tttgtcccga taaaatgaaa taaattaccg aaaataacca
    19201 aaactgcgat tggtccaatc atattcgtcg gtaatttccc taaagccatt gcaatgatca
    19261 ggacaaggac catgatcaaa taaagtggca aacttacgcc ggtgattttt gtggaccaaa
    19321 aaccttcttt tttcttttcc tcaactgcag ttgcagttgt tgttttttgt gccattttta
    19381 cagatccttt cttattctgt atcatatgaa aaaagccaaa gaaaagcgac aaaagagcag
    19441 gagttggctg gccgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt
    19501 ttctgtatgt tcttttacta taacgatctt aatcttctaa agatttatat tcaggaaccc
    19561 atttagcaga ttcaactgct tctttgatat ccgtgatttc ttcacgattc aagttttgtg
    19621 cgacaactga ttttgcgaca gtttcagcaa tcgtttgaga aaattcagtg atttttgcta
    19681 ctggaggcaa gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac
    19741 tagcttgaga aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagtc
    19801 ctaaacctgg gtacatcaaa gcattatttg cttgaccgat ttggtaagtt acaccattat
    19861 attcaacatc agcagcaggg atacctgttc caactaatgc ttttccgtcc gtccattcta
    19921 ttaaatcttt tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgatcg
    19981 gacgtggtgt atgagcagcc atttctttca cgatttcttc agtgaatgct ccaggttggg
    20041 tagaagtacc aatcatgatt gtcggatgga tcgcttttac aactgcttct aagtttgtca
    20101 attcatcagc attagtgaaa tctgagcgtt tgcgagcgaa tgggatttga ccaggagtca
    20161 atccttctgt atcttcaaac aataaacctt gtttatctac ttgatagaaa tgttggcgtg
    20221 cttcttcttc aggtacacct tgacggatca tctcatctaa aaggatgttt gcgataccca
    20281 caccagcagt accagcacca aatgtcaaaa ttatttgatc tttcaattgt tcaccggata
    20341 tattcaatcc gcctaataca cctgctagga caacaattcc tgtaccttgg atatcgtcgt
    20401 tgaaagtagt gatttgatct tcgtattttt ctaagatcgc tgcagcattt cctcgtccaa
    20461 aatcttccca atgcaataac aattctggga ataattcggt agctgattga acgaattgat
    20521 cgatgaattc atagtaagtt tcgccttcta cacgagcatg acgattgcct aagtataagg
    20581 gattattcaa taattcttca ttattagtac cagcgtcgat cgatacaggc aaaacttgtg
    20641 ctggattaat accagcagca gcagtataga ccatcaattt gccgatagcg atgtcaacac
    20701 cgttcacacc ccagtcgccc atacctaaga ttccttctgc atcagtcaca acaatcaaac
    20761 gaatgtctcg tccatctgcc gcgttcttca aacttgcttt gatgttttct ggttcgtcga
    20821 ttgaaagaaa tgcagcatct tgcggtttca agaaaatttc gttgtattgt tcgattgaat
    20881 ccgcaacgac aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt
    20941 tataaaacaa tgtccgattc gtattgaata gattcattaa gaagatacgt ttttccaaat
    21001 ctgtttgttt gcttaagtat tgaccataag cttgaacaga ttgttgttcc agtgtttgga
    21061 cagtacttgg aagcatacca gtgattccat attttgcccg ttcttctttt gtaaaagctg
    21121 ttcctttatt tagaaaagga ttatttaata agtttaaccc tgtaagcatg aatgattcct
    21181 cctaagtttt aatacaaacg aagtatagaa gtcagatgat gatatagtca aaaagagcag
    21241 gtatgataaa ttttatttat atgtaatata gccaaaagaa atcaaaatag cagtatttgt
    21301 tctttttata tcgacaatcc ttgcttgaat actatgcgta tctcttatat gctagagaaa
    21361 gaaaagtaga agaataaggg atagatggaa tggataaata ggatttctga ggaaaaaagc
    21421 acatgatagc gttgatagga gtcacctatg tcaggtgata aaaatacata agtagaaaat
    21481 taagaggagg aagagaaaat gaaagaagtt caattgatta gaaaaagcga actatcagaa
    21541 ggcggttgca atgcatgtgg cgtagtagaa gcgacaagct atacattaaa attagattcc
    21601 aatcaagcaa taatctctga actgacagta ggcggtcttg tggacagtct ggcgcttgct
    21661 gaaggtttta ctggagaaga tatttatgag atgttttcag aagtccgtca gttaaaaaaa
    21721 ggagaaaaat gcatagaggt tcatcatgag agcccgaatg tgaggttcaa acgtggtgat
    21781 aatgagatga tcttcaagaa tcatgtgtct gatcacactg aactatatga aatcgtgaat
    21841 caaatactgt ctgggatttt tgaattagga ccttatgagt tcaaggaaga aaacggtaat
    21901 ccaaaattaa atgaagaatg gcaagaaacg attgaaacac aaagaaacaa tccacactta
    21961 tttcaatagt cttccacagt atttcgtaaa aatgttgatg aaaaagaaac accgtgaatc
    22021 agaggtcgtg aaaaacctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
    22081 ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagtttttga acaccgttta
    22141 aggaagagac tgtgaagtca acgttctgac ctgagtatta gagagacaaa tggcaaaata
    22201 gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    22261 ttatcgagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa
    22321 tggctttcca catttttgca taaatttgac ttaataccga gaaaatggct tttgaacacc
    22381 gtttattcaa aaaaagggac tttgacacta cttttgtcaa agtcccatcg gttttttatc
    22441 tatttttatt acaatcattt cttccagagt agtgacatct ggaatggctg aaatgtaaac
    22501 agattatttg cctgtatcaa tgtcgaagag taatataaaa agtgtaaacg ctatcatatt
    22561 gttcatcatt caaattttat agagagaagg cattgagatg gaaaaaacat atcaagcagg
    22621 tacaaatgaa ggaatagtag attttatcaa tatggaagat ttagaaatcg cggcttcaca
    22681 agtgattcca gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta
    22741 tcaagaaaat gaacgggcat tcaatcatcg tttgattatt cctcatgtat taagagatgt
    22801 agaattaccc gatacaacta cacattttga cgaagaaatg ctgactgcac caatcatcat
    22861 ggcaccagtt gctgctcatg gattagctca tgtgaaggcg gagaaagctt ctgcaaaagg
    22921 tgtagcagat tttgggacga tctatacagc aagttcttac gcttcttgta ctttagaaga
    22981 gataagagaa gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga
    23041 taatggaatc aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt
    23101 attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa acgggtttac
    23161 cttcccgctg ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga
    23221 tgctgtatat aaatcttcca aacaaaaact tagtccaaaa gatgtagaat ttatcgctgc
    23281 tcattctgat ttgccagtct acgtgaaagg tgttcaatca gaagaagatg tctatcgatc
    23341 attagaatct ggtgctggtg gaatttgggt atctaaccat ggtggccgtc aattagatgg
    23401 ggggcctgct gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc
    23461 aatcgtgttt gacagcggtg tacgacgtgg acaacatgtt ttcaaagcaa tcgcatcagg
    23521 tgccgatctt gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac
    23581 aggagtccat caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc
    23641 tggaacacaa acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta
    23701 agaaataaat gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata
    23761 gttcaaggat gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt
    23821 catacttaat aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt
    23881 atacaaaaag ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact
    23941 gtctctacta tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga
    24001 ttgggacatt attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg
    24061 agggtcaagc gacttcaaaa ggagatatta cctttactga accaacaaat accgttgagc
    24121 ctcttaaccc tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa
    24181 ctggccagac aggttcttta acattggatg tagttcctga attgccattt ggtacacacg
    24241 agattgaaag cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc
    24301 tccaagtatc agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga
    24361 cagtttcggt ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt
    24421 ttggaacatc cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa
    24481 ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc
    24541 aaggtctggg aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga
    24601 aagcagcagc tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag
    24661 catagagtat gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt
    24721 taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt
    24781 ttcgtttttt tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga
    24841 ttcagcagga gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat
    24901 cggttatttt gatttattag tggcaccgga acaaaatcag acactagaag taattatcag
    24961 caattcttca gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga
    25021 tggtggaacg atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga
    25081 tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac
    25141 aactatacct atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg
    25201 tattcatgtt tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat
    25261 caaaaatcgt attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga
    25321 accggatctg aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca
    25381 gcttcgtttc caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat
    25441 cttttatgaa aatcagcttt atattgaaaa tacgtctaat gaatttttag ttgcaccaaa
    25501 ttcgaatttt cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg
    25561 cgcagaaatc atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac
    25621 gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca
    25681 atctttccca tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt
    25741 gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt
    25801 cgttgaaaac atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc
    25861 aagagccagt ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc
    25921 cagttccgca tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga
    25981 tttataaaga atctaatcaa catggcgtat tcccggagac gaatgaagaa aagatccgtt
    26041 ttctttattt gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa
    26101 gaaaggaaaa ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc
    26161 ttttttactt cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt
    26221 ggagcaacgc ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca
    26281 aatggtggac gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa
    26341 ttgcctaaat tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc
    26401 acggtccgtt ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat
    26461 atcactgcac aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc
    26521 agtgatgtat taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc
    26581 atcaaggtta ctagcggtag tttattatca gcaggaagcc ataccttgcg ttttgggatg
    26641 gggtcgtggg atacaaatac tctattggca gatattattg ctattattac gcgaggcgat
    26701 ttcaacgtgg acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca
    26761 gaaaacggag ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa
    26821 ccttctatgt ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc
    26881 caagatcgta tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta
    26941 actggcggaa acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg
    27001 tggaatagtt acatctcaac aagtgattca acaggaaaat ataatacatt tgctgatacc
    27061 acagaatacg tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg
    27121 acgatggggc tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta
    27181 gtcaactatt ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca
    27241 agcacaagtg gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc
    27301 ggattgcttt ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt
    27361 ttaccaacaa acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac
    27421 caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt
    27481 tattcgaaag gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc
    27541 caatcaaata acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg
    27601 caatattatt ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt
    27661 gcgtacaaat ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct
    27721 aattttccat ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca
    27781 ggagatcccc tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta
    27841 acaaaatctg gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact
    27901 acttatatca atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa
    27961 caaggagttt ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact
    28021 tacaactatc aattaaatac tgtagatact tctgtacaat ccgtcagcat actgacaaag
    28081 gcagctgtac tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt
    28141 gtccaaacat ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta
    28201 ccagatgatt ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa
    28261 gcaaaaggcg atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg
    28321 caactgacag ggacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga
    28381 acaaaattat acttcaatca ttctggatca aaacaattga ttcagcaagg tcaaaacacg
    28441 ttgatttatg agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc
    28501 gatggtttac tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa
    28561 ggcatggtca catgggaact gacagctggg ccaaccagct aagtgattag aatttgtatg
    28621 gatctgtgat aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt
    28681 aaaatcagaa atgcttggag gagtggactt ggaacttttc agtttgcttg aaaaaaatga
    28741 acaacggcaa atcaaacttt tccaactttt attagaggaa aaaggagaga tttccgttaa
    28801 taacgtactt acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt
    28861 acaagctaac ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt
    28921 attaatcaga catggaaata tagcagcagt cgatgtttat tatcattgct acttgcgcta
    28981 ttctacaaaa tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa
    29041 acttgtcatg gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa
    29101 tgattcttta aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag
    29161 tcaaatttgt tatttttatt tccaattatt ttggtttgga cgtccttatc ttgaaaacaa
    29221 aaaagaatac agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag
    29281 ttttactatt acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa
    29341 gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt
    29401 tcaaacgaca caatttttat tggacctcca tacatttttg acccgttact tctttcaata
    29461 tgcctttcct tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt
    29521 tacattagat acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga
    29581 cgacgtagta gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt
    29641 ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc
    29701 tcattttgcc tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg
    29761 gttaactgaa gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac
    29821 atttgctatt gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc
    29881 tcgctatgca tctattttgc ggacgctgat caagcagtta gaattacgtc tgcaaattgc
    29941 ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca
    30001 attgctgaat acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat
    30061 cattttgact agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt
    30121 cactagcaac gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta
    30181 tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc
    30241 catgcaggca tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta
    30301 ctgatcgatg aagatcataa agatggacta ggctatgttt caaatggaat caacctttat
    30361 ttaaaaggaa agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa
    30421 agtggggcaa aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag
    30481 ctcatcctcg catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta
    30541 gcaacaggtt cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc
    30601 tataaaaatc tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta
    30661 gtgatcttcg gtgctggtta tattggattg gaattagcag atgctttgag aaataaagga
    30721 tacattattc atctagttga ttatatgccg aatgttttat caagatattt cgataaggat
    30781 atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa tcaattttta tccaaatgaa
    30841 ttcttgatcg attggaaaaa atcagaagag aaagtagtat ccgtacaact tctatctcaa
    30901 gcaataaagg cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta
    30961 aaagaaaaag tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca
    31021 agtgatccgg acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa
    31081 cggcatctct ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta
    31141 actttgagtg gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt
    31201 atagactact ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa
    31261 aataccggtt cgtgttcagg agaatttgat ctatttccac aatacgatga agaaaacaaa
    31321 acagtaaatg caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg
    31381 ataagccaag aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa
    31441 actacgattc cacagctagc agttgaggat tttggatttc tgtctgaaca cacctctcgt
    31501 tttcactacc tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga
    31561 gtagataaaa gactgtgaga aatatcacgg tcttttattg tgt
//
LOCUS       DANKHK010000025        29213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000025 DANKHK010000000
VERSION     DANKHK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(56..1351)
                     /gene="purB"
                     /locus_tag="PW109_001035"
     CDS             complement(56..1351)
                     /gene="purB"
                     /locus_tag="PW109_001035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2510898.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1364..2488)
                     /gene="purK"
                     /locus_tag="PW109_001036"
     CDS             complement(1364..2488)
                     /gene="purK"
                     /locus_tag="PW109_001036"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2510899.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2481..2969)
                     /gene="purE"
                     /locus_tag="PW109_001037"
     CDS             complement(2481..2969)
                     /gene="purE"
                     /locus_tag="PW109_001037"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2510900.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3385..4692)
                     /locus_tag="PW109_001038"
     CDS             complement(3385..4692)
                     /locus_tag="PW109_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2510901.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4695..5276)
                     /locus_tag="PW109_001039"
     CDS             complement(4695..5276)
                     /locus_tag="PW109_001039"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2510902.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5392..5489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5848..7140)
                     /locus_tag="PW109_001040"
     CDS             complement(5848..7140)
                     /locus_tag="PW109_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2510903.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7137..7760)
                     /locus_tag="PW109_001041"
     CDS             complement(7137..7760)
                     /locus_tag="PW109_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510904.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7763..8599)
                     /locus_tag="PW109_001042"
     CDS             complement(7763..8599)
                     /locus_tag="PW109_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2510905.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8616..9503)
                     /locus_tag="PW109_001043"
     CDS             complement(8616..9503)
                     /locus_tag="PW109_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2510906.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9519..10802)
                     /locus_tag="PW109_001044"
     CDS             complement(9519..10802)
                     /locus_tag="PW109_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2510907.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11485..12168)
                     /locus_tag="PW109_001045"
     CDS             complement(11485..12168)
                     /locus_tag="PW109_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2510908.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12186..12803)
                     /locus_tag="PW109_001046"
     CDS             complement(12186..12803)
                     /locus_tag="PW109_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2510909.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12813..13637)
                     /locus_tag="PW109_001047"
     CDS             complement(12813..13637)
                     /locus_tag="PW109_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2510910.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13630..14190)
                     /locus_tag="PW109_001048"
     CDS             complement(13630..14190)
                     /locus_tag="PW109_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510911.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14364..15320
                     /locus_tag="PW109_001049"
     CDS             14364..15320
                     /locus_tag="PW109_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2510912.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15625..16581)
                     /locus_tag="PW109_001050"
     CDS             complement(15625..16581)
                     /locus_tag="PW109_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2510913.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16685..17287
                     /locus_tag="PW109_001051"
     CDS             16685..17287
                     /locus_tag="PW109_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2510914.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17375..18874)
                     /locus_tag="PW109_001052"
     CDS             complement(17375..18874)
                     /locus_tag="PW109_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2510915.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18890..19414)
                     /locus_tag="PW109_001053"
     CDS             complement(18890..19414)
                     /locus_tag="PW109_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2510916.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            complement(19500..19685)
                     /locus_tag="PW109_001054"
     CDS             complement(19500..19685)
                     /locus_tag="PW109_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510917.1"
                     /translation="MAETIKETNVEKQTTRMIVFWSFNGFFLKKGNIGYLERIYCENF
                     NMKTKFNKLSTINTTFL"
     gene            19711..20460
                     /locus_tag="PW109_001055"
     CDS             19711..20460
                     /locus_tag="PW109_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2510918.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20451..21236
                     /locus_tag="PW109_001056"
     CDS             20451..21236
                     /locus_tag="PW109_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2510919.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21205..21921
                     /locus_tag="PW109_001057"
     CDS             21205..21921
                     /locus_tag="PW109_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2510920.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22023..23057)
                     /locus_tag="PW109_001058"
     CDS             complement(22023..23057)
                     /locus_tag="PW109_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2510921.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23061..24119)
                     /locus_tag="PW109_001059"
     CDS             complement(23061..24119)
                     /locus_tag="PW109_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2510922.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24340..25488)
                     /gene="nagA"
                     /locus_tag="PW109_001060"
     CDS             complement(24340..25488)
                     /gene="nagA"
                     /locus_tag="PW109_001060"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2510923.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25564..26400)
                     /locus_tag="PW109_001061"
     CDS             complement(25564..26400)
                     /locus_tag="PW109_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2510924.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26584..27801)
                     /locus_tag="PW109_001062"
     CDS             complement(26584..27801)
                     /locus_tag="PW109_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2510925.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(27950..28129)
                     /locus_tag="PW109_001063"
     CDS             complement(27950..28129)
                     /locus_tag="PW109_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510926.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            28184..28267
                     /locus_tag="PW109_001064"
     tRNA            28184..28267
                     /locus_tag="PW109_001064"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28218..28220,aa:Leu,seq:gag)
     gene            complement(28507..>29213)
                     /locus_tag="PW109_001065"
     CDS             complement(28507..>29213)
                     /locus_tag="PW109_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510927.1"
                     /translation="TPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMN
                     ILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMI
                     WSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVIL
                     MIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFG
                     LTVLGICTFIITVINR"
BASE COUNT         8704 a         6245 c         5021 g         9243 t
ORIGIN      
        1 ttttttggtt atttttgcta ttctatttta attgaatttt gcaactcact cttctttatt
       61 ccaaacctac tcgtttgaat atcacgtcaa cgtttttcaa atgatgatga taatcaaagg
      121 catcgtctat ttcttcttta gataataccg atgtgatggt tggatcttct tctaacagcg
      181 ggcggaactg cacttggttg tcccaagcgt acgcagtttt tggctgaaca agatcataag
      241 cttcttctct tgtcattccc ttatcgatca atttcagcat aacgcgctgg ctataaatca
      301 aaccaaatgt tgcagacata ttccgcttca tgttttcagg aaagaccgtt agatttttaa
      361 cgatattgcc aaaacgattt aacatgtaat tcaataaaat cgtggtatca ggcaagatga
      421 ttcgttctgc tgaagaatga gaaatatctc tttcatgcca taatgtgaca ttttcatagg
      481 cagtgaccat gtgtccgcgc accactctgg ctagaccagc catattttct gaaccgatcg
      541 gattcctttt gtgcggcata gcagaagaac ctttttgacc tttcgcaaaa aattcttcta
      601 cttcacgtgt ttcggatttt tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg
      661 tagcaataag cgccatcgct gagaaatatt cagcatgtag atcccgaggg agaacttgag
      721 tagagatttc ttgtggacgg atccccaatt gtttacatac atactcttca ataaatggcg
      781 ggatatttgc gaacgttccc actgctcctg agatttttcc tgcttctact ccttttgccg
      841 catgttcaaa tcgttcgatg ttccgtttca tttctgaata ccatgtggca agtttcaatc
      901 caaaagtagt tggttcagca tggacaccgt gtgtccgacc catcatcact gtatatttgt
      961 gttcttttgc tttttcgcca atgatttccg taaaacgttt caagtcttcc cgtaaaatat
     1021 catttgcttg cttgatgaga tagccataag cagtatctac gacatccgta cttgttagac
     1081 cataatggac ccattttctt tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa
     1141 cgacatcatg gcgtgttaat ttttcgattt ccaagatccg atcgatatca aatgatgcat
     1201 gctcacgaat ttttttcaca tcttcttgcg ggatttcacc aagttctgcc caagcttcat
     1261 ctgctaaaat ctcgacttcg agccacgcac ggtatttgtt ctcttccgtc cagatattcc
     1321 ccatttcagg gcgtgtataa cgttctaaca tgttttttct cctctaatcc caaatattcg
     1381 tttgtttgat ttcttccagc gtttgctgga tgtcttctgt caagatagtg atatgtccca
     1441 tcttgcgacc tttttttgct tgtttttttc catagaaatg gaattgccag tctggcttat
     1501 tctcaatcaa gcgatagctt tccagcagtt gttctcccaa aacattgacc attactgcat
     1561 tgcttatcaa acgtactttt tctcctaatg gccaaccaca aatacctcga atatgggcat
     1621 cgaactgact catagaacaa gcttcaatag aataatgtcc agaattgtga ggacgaggag
     1681 ctaattcatt cacatatagc ccgcctgttt ttgttaaaaa catttctacc cctaaaacac
     1741 ctgaaaggct cactgcttct gcaatcacac gggcaatacg ctcagcctcc tcgatcacat
     1801 cctgatcaat cgcggctgga gcaatcgttt catgaagaat attgttgtgg tggatgtttt
     1861 ccacgaccgg aaaagtcgtg aaatctcctt gaccatttcc tgccaccatg atcgaaatct
     1921 ctttttcaaa cgggatccat gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg
     1981 caggagctag atccgcccga cttttcaaaa catactgccc cttgccatca tagccgcctc
     2041 gagtggtttt caaaacacaa ggatagccga tcccgtcaat cgcatcttgg atatccgtcg
     2101 gactgacgat cgttgcataa ggagcaatca cgatgttatt cgtttccaaa aaagattttt
     2161 ctaacaggcg gtcttgcgta atcgcaagca agtctgtccc ttgaggaata aatgacatgg
     2221 gtaaaatcgc atttaacgcc tctacactga cgttttcgaa ttcataggta atgacatctg
     2281 ttcttctggc aagctctttc agcgcaaagg tgtcatcata gacactttca atgtgccaat
     2341 ctgcaacttg tgcggctgga caatcgctca caggatccag cacacctact ttaaagccca
     2401 tttcttttgc actaatcgcc atcatacgac ctaattgacc gccaccgacg atcccgatcg
     2461 ttgaaccagg caacaaaggt ttaaccaagt tgatcactac tttctataac agttttagcc
     2521 aacattgaac gtctttcagc caatttagcc tccaattcta aatcatacat ggaaagcatc
     2581 tgtatcgcaa gtagtccagc attaattgct cccgcttttc cgattgctgt agtagctaca
     2641 ggtactcctc caggcatttg gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt
     2701 gattggaccg gtacaccgat aaccggcaaa gttgtttttg atgcgaccat acccggcaga
     2761 tgcgcggctc cccctgctcc tgcaataatg attttgattc ctttttttcg agcgttttgt
     2821 gcatattcaa acatcaaatc tggggttcga tgagcagaaa cgaccttttt ttcataagac
     2881 acaccgaatt catccaatat ttgacatgct tctttcattg tttcccaatc tgaagtactc
     2941 cccataatca cagagatttt tgctgtcatt gtttttcctc ccagtttttc tacattcata
     3001 ttttaacaat cacttttttt gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt
     3061 ttattgttcg tgtttattaa agataaaaaa aaggaatgtg acaaaagtta ggtcacattc
     3121 ctttttatgg ataaacggtg ttctcctgcc aactcctcgg tcacaaggaa caatattcaa
     3181 caaacatgag aagctttttt ttgaaattgt tccttgtgac tcggagatga acggcaggct
     3241 cacgacctct tccttcaacg gtgttcaatc agctgaccct cggtattagc tcaaacaaac
     3301 ggcaaaatac gaggaactat ttttccgttt gtttctctaa caatcaggtc aaaacgctga
     3361 tttcacaacc tttcttttgc tctattactt cttgaaggct tcttccaata tttcttcatc
     3421 ttttttcaat ccattaaaga tcaaattcaa tatgatagcc gtaatgctac tcattacgat
     3481 gccatttcca gtgaacattt gcaatgtttg cggcatttga ctgaataatg tcggcgtaac
     3541 attgaaaccc agcccaaatc caacagaaac agcaacaatc aatagatttt tatcattgct
     3601 gaaatcaact tccaacagca tacggattcc ttggactgcc accatcccga acatgactaa
     3661 cattcctcca ccaagtaccg gctcagggat gatttgtgct aacgcaccga ttttagggaa
     3721 taatcctaaa acaatcaaga agaatgcaga gaaatagatg ggttttcttg ttttgattcc
     3781 cgaaagttga accaatccga cattttgaga aaagccagta taagggaatg tattgaaaat
     3841 accgcctaag ataacggcaa gtccttctgc acgatatccc tttttcaatt cttcttctcc
     3901 aacatttttc ccagtaatgt caccaagtgc aaaatataca cctgttgatt ctaccatgct
     3961 gaccatggaa atgatgatca ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa
     4021 ataaaagggt tgtggaacat ggaacaacgg cgcatctcca atcgcgctca gatcaatcat
     4081 tcccatgaaa gcagctaaga tgcttcctcc gactaaacca atcaataccg cgattgaacg
     4141 aatgaaccct tttgccagca tttgcacgcc tacaattagg agaatcgtca caaatgcaag
     4201 tagcagattt gttttgtcac caaaatcagc tgctgttgca ttacctccac ccattttctc
     4261 gattgctaca ggaatcaaag tcaaaccaat aacggtgata accgttcctg ttactatagg
     4321 agggaatagt tttttaattt tagaaaagac accagaaact aacacaacaa agatcccaga
     4381 agcgataata gatccgtaga tagctcctac tccttcattg gttccgatca agatcagtgg
     4441 agctactgct tggatcgcac atcctaaaac gacaggcaag ccaattccga aaaatcggtt
     4501 aactgtcaat tgcaataatg tcgctaatcc acacataaag atatcaattg aaatcaaata
     4561 agtcatctgt tcttgattaa agcctagtcc tgtaccgatc aaaagaggca ccgctactgc
     4621 tcctgcatac atcgctagca gatgctgcag ccctaaaacc gcagcttttc cattttgtgt
     4681 ttctttttcc acgattatgc gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa
     4741 gcaatacgcg caagagaaac gactctcaat cccatttctt caagtaactg acgtccatct
     4801 tggaaagact tttcgatgac gatcccgatt ccttctactt gcgctccagc ttgctggcat
     4861 aattcgatca atccttttgc cgcttgtccg tttgccaaga aatcgtcgat aatcaatact
     4921 ttgtcttctg aagataaaaa tttacgagaa atagaaatcg tacttgtcac ttgtttcgta
     4981 aaagaataaa cagaagctgt caataattct tcatccatcg tcaaactttt tgcttttcta
     5041 gcgaagatca ttggaacatc taattctctt gcagcaaata aagctggtgc gatcccagaa
     5101 gcttcaatgg tcacaacttt cgtgattcct tgttctttaa atacttctgc aaaccgtttt
     5161 cccatttgtt ccatcaatac agggtctact tggtgagtga cgaagctgtc gacttttaat
     5221 acgccctcgc ctaatatacg tccgtctttt tgaatacgtt caactaattc tttcacttta
     5281 tttgctcctt tatcatttat taataccgaa ataaccgctt ttgtgcaaaa acctactatg
     5341 tttctgttaa agaaaaacac cctccacttt atcataagaa aagaagggtg aaatagccaa
     5401 tcttttactt atagtccagc atttacggta ctggctagaa actgtcatcc atattatgac
     5461 gatatataag taggtatgta aaactaataa tagtgtaaat tcattttctt gcaactgttt
     5521 tgcacgtgaa ggcagtttgc catatttgaa taaaaaatgt tgtttcaaat attgaaaaca
     5581 gtctaacact actttctgaa actgacaacg tggtttactt atttttttgt aaaatctaat
     5641 gatttttaat gcattattcc tttttgttcg ggtttaaata gttattttta ttttctccta
     5701 tctaaaaaag cagcccacat aaaaagaagg catgacaaaa agcatgtcat agcccctttt
     5761 tccgaataaa tagtgttcaa aagacagcct atcggcaata aggcaagatt ttataaacta
     5821 cgggaagtaa tttccgtaaa tctattctta ttcttttaag ctgacaagct cgtcaatcac
     5881 tactgctttt cctgaagcaa tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat
     5941 accatctacg tgagttgctg cccgattcca cggatcttct tctttttctt caccaaatag
     6001 atcatctaat aaacgcggat ctcctccgcc atgtccgccc gcttcttgtt cgacgattac
     6061 ctcatatggc tcaccgaaca tcggatagac atggatggac tgaagaacgg ctgctccttc
     6121 atccttttta tctccacctg cattgatgta agacttttcg atcactttca tttcgatccg
     6181 tcctttgctg ccattgaaag aaacattaaa tccttcccac ggcatatacg cgtttagcga
     6241 atacgtcaaa atcgcaccag tttcatattt gaccgttacg cctagtgtat cttcaataga
     6301 gatatcatcg gaaaatacac tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa
     6361 atacatttct cgcaattcct cattggctga caggtctata gcaaatggat cagctttagc
     6421 tagttcgctt ccatagcatc gactgtaaaa tatttctact ccgcgtttct ccgcattatg
     6481 catcccataa aagtttaatc caccgaatgc aaataccttt tggggacggc tgttcaacca
     6541 aaaattcact agatcaaaat gatgggtaga tttatgaaca agaagcccgc cgctattgtg
     6601 cttatttcga tgccatcttc tgaaataatc tgccccgtgt tgcgtattta acagccattc
     6661 gaagtggata gaaaacacat caccaatcac atcactttct atcagttcct ttatcttcgt
     6721 gtgatacggt gcataacgat aattaaaagt gactcgcagg cttcttcccg tctgtttttg
     6781 gaccgttatt atttcttgag ctttttcctc atcgatagtc agaggttttt cgcaaataac
     6841 atcatatcct tttttcattg cacgaatgat ataatcatga tgcgttcgat ctactgacgt
     6901 aataatgact tcagccggcc gtacgtcatc cagtagttgc tcaaattgtg atggcataca
     6961 tgtctttact tttttctgtc catacttgtt cacaagtatc cgatttgcat aatccatcct
     7021 tgtttgagat acatcacaaa aaccgactag ttcacaagtt tgatgatacg tagaggcaat
     7081 tgcctcataa aaaaaacgcg ctctcccacc aagtccgact tgtacatatt ttttcttcat
     7141 ttaaagtcat tctccttttc ttttaagcaa aatactgtga caaatggcag ccctggatac
     7201 agtaatgcag tgagtaaaaa atcatgctgt atacaccaga acagtccata caaaaggcat
     7261 aaataccaca gaaacccctt gacgtttttt gacaacagcc agatattcct tttgatcaaa
     7321 taaacaagac tatctgcttt atcttctttg cagatatcaa ttagcgtact aaaaaaacta
     7381 tgggatacaa aaagcaacag acacaataga aaaggaatca gataccaagc tccaatcttt
     7441 cccactaata aataccaatc aactgcaaaa ataatgacaa acagctgaaa caacgtaatg
     7501 atgaaacaca tccgtttgat ttttcctttc ctccaacata cctttttttc tgccaagtaa
     7561 tggacagata gataataaga aggatacatc atcaaaaaat aaatcagata gacaacacga
     7621 gcagacagca ctaatcctaa agtgaacaaa catcctagaa ctaaagcgaa aagagcatta
     7681 ctgataagac tggttaaaaa aatccgacag acatagtagt tgagcttgta aagaaacatc
     7741 tcttctattg gctttttcat attcagccct tcagaccgct tgtactgatc ccctggacaa
     7801 gatatttatt gaagaataag aaaatcagaa aaaccggtag cagcgacaac gtagacatcg
     7861 caaacatact cgcgtaagtc gtactagatc cagcgtctgc aaataatttg atggcaatcg
     7921 ataccgtgta agatctcggc ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt
     7981 tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa
     8041 ttttggtaaa gacactatac ttgctgcatc cgtcaataat cgctgattca tctaactctt
     8101 tggggatgct gaccatgaat tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat
     8161 gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag ccgattgtaa ataataaact
     8221 gcggaatcaa aatcacttgt gcaggaatca tcatcgttac taacatgacg acgaaccaga
     8281 atctttttcc tttgaaatcg attctcgata atgcgtaagc aatacaagtc gagctcagaa
     8341 ctgtccctat cgtcgaaact gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg
     8401 aaattccagc aaaccccgcc catccttttg cgtaattttc ccattgaaaa gtctctggaa
     8461 tcaaattcag cgaaccactc ataatctctt gatccgtttt aaaagaacct gcgatcatcc
     8521 ataagaccgg ataaatcatg actagaccta atagcaaaat aaaaccgtat tgaaaccatt
     8581 tcgttactgt attattcatt tcttcctcct ctcattcaca caacgaccca tttttttgat
     8641 gatttgaaaa tcagaaaggt gacagctccc ataatcaaca gcatgatcca cgaaatagca
     8701 ctagcatacc ccatagaatt aaaagaaaaa gcttggttat acacataaag agcataaaag
     8761 ttcgtggcat cattcggacc accttgggta atgataaatg cctgcgtaaa agtcataaac
     8821 gccgcgattg tctgcataac caagttatac aaaatgatcg gcgaaagaag aggtaacgtg
     8881 atcttgtaaa acgtctgcca tttgttcgct ccatcgatct ctgccgcttc ataatacgag
     8941 cctgggatct ctttgatacc tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca
     9001 gacatcaaaa caagagggat catgaccagt ttttgatcgc taaaccagga aagcgcaggt
     9061 gcgcctaatc gttccaggaa aatattcact aacccgtttt tagaaaacaa ctcccgccaa
     9121 actagagcga tagaaatact gccaccgatc aaactaggca cataatacaa agaacgaaag
     9181 aaagtcgttc ctttgatatt cctcgttaaa agataagcaa tcaatagacc aaagatcagt
     9241 ttcacaggta cagatgcaaa tacatagaga aaagtgactc ggatggagtt caaaaaacgg
     9301 gcatcactca gcatctttat ataattatcc aagccgatcc atgagactgg atttcccaaa
     9361 cgataatcgg tgaacgaata ataaagtgac aagatcatcg gtatcactgt tatcaataga
     9421 aatcctagga tgaatggaga agcaaatacg taaccgacca cattgtcttg atccaagaac
     9481 cgttttagtt tttctttttt catgccttcc tcctgctgct atcctagaat gtttgacgca
     9541 aactcataaa gactctctgc tgcttcttct gctgttactt cttcataaat gactttttca
     9601 actaaaagat tgtattggtc ttgaatttcc ggattcaaag cactatcaaa gacattcact
     9661 tcgccgttgt ccatatcgga aatcatttct aaataatcaa atgtcgcttg caaagttgca
     9721 tcatctgcac gagcgagctg ttctcgaata ttcgacatga ttggtacacc acgttctcct
     9781 tttaaaattt cattggcttc ttcactgttt tgaaagaaat caataaactt agcagcttct
     9841 tctttatggg ctgagctttt tgccatactc agcatttgcg aagaacgtaa agggatacca
     9901 gattctccat ctgcttttct gcgaggtgga ttaatcaact gaatctccat atcttcagga
     9961 gcccctgaca atatttctgt caactggtta cttgatagaa acaccattgc cgcttctcta
    10021 tccctaaccg cctgaaaaga cagatccgtc gtacttgcag ctccaccacc tggttctgga
    10081 tgggcccctt tgtctaccag atttttacgc agctggaaaa aatctgttag atagtctgga
    10141 ttttcaaatc ccaagctgtt agatagatct tcccgattat aaaaattcaa tcctttctca
    10201 acttgtggta tttgaataat acagccagct ataaaatctt cgaatcttga aatagcatag
    10261 attcctaatt cttcagtgat ttgatagctg atttgttcaa attcatccca tgtccaattt
    10321 gaagctggtt cagggatatt taattgttca aatatatttt tattgtaagc aatcgcaaac
    10381 gaattcaccc cgttagacag tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag
    10441 gattcatctg cattagaaat atcaattatc ccttgctcca catattcatc taatggttca
    10501 atctgattga tgtattgagg aaattgattg ttcagttgga aaatgtccca gatatcatct
    10561 gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat aatcataaaa ttcataatcg
    10621 atcgtcacat ggggattttt ttcttcataa agctcgatta cttttactgt tgcatcgtgt
    10681 cggacttgcg aaccccacca agccatgttc agcgtgattt ttttgtcatc ttcttgccca
    10741 caagccgtca agaagaataa cataacactt aaagctaaga tgattctcat tcttttcttc
    10801 attctctgca cctctacttc cattgttatt tttcaatata ctgtattttt atacagaaca
    10861 tatttattaa acgatctttt tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc
    10921 ctaggatgaa caactcaata ctttcaagaa aaattagata gtgctgattc ttctttcatc
    10981 gctaaaaaca cttcttgctt caatgatggg aggataatta cgcaaagaaa aaaggatatc
    11041 agacaactgc ccaatatcct tgacatagct aaataagcat gcttcttcgt gtgcgctctt
    11101 ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc
    11161 gatgcacaag cattgactat ggcaacttga ttggtcagtt cccgctgact tttcaagccc
    11221 cgacaattta gctgctacta aattgtcggg tgcctttttt agtttttatt cgtttttcat
    11281 tctatagaaa gcgcttcaat aaatcaagac atttttttgt tagaatgcta ataataacaa
    11341 aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt
    11401 ttgaattctg aacaaatagt actggagtac aaaagacgag gaagagccac tatctgctct
    11461 tcctcgtctt ttgattctca cctgttattc atgattcttt ttctcagttt cccgatattt
    11521 ttccatttta cgactaaaaa gctctccgct acttggtggc gtatatgtga tggcatttgc
    11581 tcctgcttga atggtctcag caatactttc ttccgttgga ccacctgtcg caatgatagg
    11641 aagttcagga tattttttac gaaaatggcg tactgcttct gcagtttctt tacctgcact
    11701 gatattgata atcgaaatac cagctgctaa cttttcatct atttctgtat atttcgacgt
    11761 taccgtactg ataactggaa tatcaactac tcgacaaact tcttttactg tctctaaagg
    11821 cgtaggaccg ttcaaaacga cccctaacga accttgcgct tctgcgaaca tgctcatgta
    11881 ggaagaacga gcaccttgtg tcaaaccacc acctacaccg gagaaaactg gaatgtcagc
    11941 tgcctcaata atgcttttag taatcgctgg atgaggtgta aaaggataca cagcaattac
    12001 tgcattcgca tttgtattac gtatgatcgc aatatccgta gtaaagatga tcgaacggat
    12061 caattttcca aatatcttga tcccgcttgc ttctaaaatg atttccggta ctcttacgat
    12121 atcttttctc aattccgtca tgatttctgg cgtaaattgt tctgccatca gccacttctc
    12181 ctttgctatc tccggtcata ctgattcaat tgatacgtct cgatgacctg gatgattttt
    12241 tgcgcataac cggggtctgt cgcataacct gcatcttgca atgcttgtgc ggcttcctga
    12301 tagttggtgg ccgtaatcac tttttcatac agcgcaggat tccaatctac tccttggacg
    12361 aacagttgtg tatggtcatc catggactgt tcccatgaat catacacttt gaagtctcct
    12421 tggatagtta tccattcttc gttaacaaat tcctttgttt ccaggcttac tttcttctga
    12481 tccccataag ccttgatccc gaaaagatta ttgtatttgc tagccaaggt actttttccc
    12541 caatttgatt ctaatatagc ttgtcctaaa atgatactag gaagcactcc ataaccatct
    12601 tgtagttctt gggcatgcgg aacaagccgt tcaataaatt gctgatggga ttgcgcttca
    12661 ttttcttgct gattatcagt aaaaggagaa gacagattct tcaaggaaaa aacaaaggct
    12721 aagagtatca ctgctacacc ggcaaccact gctggccaac ggatattctt gcgcttttgc
    12781 tttttccact tttttttcgc catgatcaat tcctactttt catttaattc attcagatat
    12841 tcttctagcg tcaattggtg tttggtgata tactcagcgt tttctttacc gacataacgt
    12901 aagtgccaag gttcatatgt gatcttagtt atatcttcgt gtccttttgg ataacgaacg
    12961 ataaatccat actgcgcagc attatctgcg atccatttag ctccaggctg atctccgtaa
    13021 cttgcatcca atacagtaga aggataactg ttgtaccagt tttgatcgac gacatcgact
    13081 gccagacctg tgtggtgttc gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt
    13141 gtcgcctcct cttctgatac accttgacga ctcatcacat cttggacatt ttgttcgaaa
    13201 acttgttgtt gggatgatac agaccggtaa gctgagacca tcactaatgg aaatccagct
    13261 gcttcagcag ccttggcaaa tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat
    13321 ccgttactta atgatgccaa ttgagtagac tcatcaattt ctttttctaa tttgtgatct
    13381 ggaccaacca aaactagctt ccaatcgtct gttttaacat ctggcaattg tttggacgta
    13441 cgatcttttg tttctttgtc ctttaccttg ctgctggttg tttgactgtt cttttcttta
    13501 ctagcagttg ttttggtgct tgttcgttcg actgcttgat tgccggcttg aacatgcgaa
    13561 tcatcatcgc taaaaataac atagcccatt gctgcgatca taatcacaac tgctccaaat
    13621 cctaaaaatt tattcacttg aaaaatcctt tcgttgttct tcacgttccc cattcgcctc
    13681 atgaacgtaa gtttcttcca gattttcatt cacccaatcg agaagttcga ttttttctcc
    13741 ttcgatctgt ttttgaacat ctgctggcag acgaaaattc aaaatatcat catagcccca
    13801 ttcatcatat tgaatactta ctttcaagtc aaggtatcta ccagcctgct gattattttt
    13861 caaagggatc agctcgactt ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt
    13921 tttgatttgc ttgtactctg caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg
    13981 acgcaaaatc ttttgcgtta gcatccattc ataatcagtg aaaagatttt ttactttctg
    14041 catcgcttca gatggcagat ttttctcgcc atctttccat ttttgccatt gttctggact
    14101 gatcagcaag tattcatcta aaaaattttt ttcagtttca aactgttcta ataatacact
    14161 cataataaga tcaatatata cttgatgcac gtttatcccc tccttttaac tattctactt
    14221 aagttttgat caaaaagcac ctcttaaatc gctgctttca ttttttttaa tgtttcaagt
    14281 cctctcctct ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc
    14341 caactatgaa ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta
    14401 ccatcgcaca tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg
    14461 cttccagaac ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt
    14521 acggaagcta tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac
    14581 caaatagaca gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat
    14641 gtgaaaaagc catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag
    14701 aaaagaattt gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt
    14761 tacgctcaat aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg
    14821 gcagttacaa agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag
    14881 ctctgttgga tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac
    14941 ctgaagtagt tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca
    15001 ctatcttgcg taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa
    15061 tgccaaaagt cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc
    15121 gtgctgatcg tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga
    15181 atacatctga agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact
    15241 tgcccaatcg ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag
    15301 ttctatggaa aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat
    15361 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca
    15421 gaggaaagct tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag
    15481 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
    15541 cgaggagttg acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct
    15601 gaatattagc tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt
    15661 gatttcaata aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa
    15721 ttcagggcga ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg
    15781 tgttaaaatt tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa
    15841 accatagata tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat
    15901 gtctttaatc acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg
    15961 ctgtctgagc cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa
    16021 tgtcagtaaa tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc
    16081 tgccatttgt ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag
    16141 ctgcttcgta cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc
    16201 cattaatccc gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg
    16261 agtgaccaaa accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg
    16321 gatgaacagg aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact
    16381 gaacagtgct gttgccgtag ctaatataaa ggcggcgatt cgattcatgc ccgtacgcaa
    16441 tgtagagcgc ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata
    16501 caacagcgat aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac
    16561 tgttcgcatt cctatacgca tttacttccc ccttttttat tttaattata cttgaataat
    16621 gaaaaactag ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat
    16681 ctctatgcaa tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt
    16741 ttttttatgt atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt
    16801 atctgctgtt ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag
    16861 gatcatcggc aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta
    16921 ttttcacaat gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat
    16981 cgtcgtgtca gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt
    17041 gctgattgcg ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact
    17101 agatactttt atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga
    17161 aacagaaaag aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt
    17221 gctaagaaca aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga
    17281 ggactaattt tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc
    17341 ctccattttt gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt
    17401 ctgcctcttt tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat
    17461 tctctacgat ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag
    17521 aaagcaaatt tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc
    17581 ttcctgtatc gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca
    17641 tcaattgcgg tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga
    17701 aggcattggc gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt
    17761 cggcttctgc ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca
    17821 cacggaatcg ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt
    17881 gttctttagc gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt
    17941 cacgacgtgt gatttctttt tcttccagtt cgatttgttt ttgacgttct acgattttaa
    18001 tctgcatttc ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt
    18061 ggtctgcttt tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt
    18121 tttcttttaa tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt
    18181 tttctgcttg agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg
    18241 catctctttt gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt
    18301 ctctgacctc tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag
    18361 aagcgacttc ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag
    18421 tcatagaacc aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca
    18481 gttcttctct tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg
    18541 aaccgatttt gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca
    18601 cttcaggggt ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa
    18661 aaactggcaa gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat
    18721 caatgtgcac atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt
    18781 ttgctgtctg gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga
    18841 ttgcgatcgc gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta
    18901 tcatgctttc ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt
    18961 tcgttccccg ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg
    19021 aaccggtaac acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca
    19081 caacagctac ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt
    19141 tcatcggcat caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac
    19201 tagcaataaa taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata
    19261 agcttgtgaa agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga
    19321 atacatctcc aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc
    19381 aataaatcgt ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt
    19441 ctatcatacc aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt
    19501 tataggaagg ttgtgtttat cgttgacaat ttgttaaatt ttgttttcat gttaaaattt
    19561 tcacaataaa ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag
    19621 ctccagaaca cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct
    19681 gccatattga tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc
    19741 tctagcattg ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt
    19801 gacccgacac atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat
    19861 tttatcgttg tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc
    19921 ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc
    19981 tctgaaaaag aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat
    20041 caagagatta ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc
    20101 aaagatggac aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata
    20161 ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa
    20221 atagcagaac agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta
    20281 gccatctctt ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac
    20341 caaacagagc agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg
    20401 ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa
    20461 atgggataga aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag
    20521 cagtcgcaac tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga
    20581 cgaaacaata tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg
    20641 tcatttctgg tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc
    20701 taaatcaaaa ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa
    20761 ttgccggttt tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt
    20821 tagaaagcga ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc
    20881 aagtgttagg gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa
    20941 acggacaggt ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg
    21001 tctgcgatga cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt
    21061 tttcgacgaa gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac
    21121 gccacggtgg atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag
    21181 aattaccaaa aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca
    21241 atggttgcag aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa
    21301 agtttcagaa atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg
    21361 ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag
    21421 caaggaaatg atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag
    21481 aatgtgatcc gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt
    21541 cagctggcga tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc
    21601 gatcagaaat tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca
    21661 ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt
    21721 ctaaaagaag atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt
    21781 atatccgtca agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc
    21841 aaatatttgg aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa
    21901 aagctcacag attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca
    21961 gaagtaacaa ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt
    22021 tcttatctta attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct
    22081 attatttcac gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta
    22141 tttgaaccta catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata
    22201 gcagggtctg gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt
    22261 gcaaaaacat cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct
    22321 tcattatctt gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga
    22381 gctaaaaatc cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata
    22441 ctgacaatat cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta
    22501 gcagcttctg tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa
    22561 acagcttccg tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt
    22621 tcacgactaa acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta
    22681 tacgcagtat ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt
    22741 ggcgaatacg atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg
    22801 atatgattga ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt
    22861 gcaagattgg tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt
    22921 gctgacacga tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa
    22981 atattcaatt tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc
    23041 atactttctt cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca
    23101 acgatccata caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag
    23161 gctaaaagtg cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt
    23221 gtagccatca tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt
    23281 gccgtcaaaa ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga
    23341 ctgtaatacc atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt
    23401 gcgccgattg ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga
    23461 aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct
    23521 gcacctgtaa ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca
    23581 aaagtagcga taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc
    23641 aagccactca tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa
    23701 attgaggaat acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa
    23761 aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag
    23821 cttaagaaaa aaggttttga aactagaata agaataatat aaataactaa tccccaaatc
    23881 aagacattga ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat
    23941 gccagtgctg gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca
    24001 gaaaaaatcc ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg
    24061 attgcactga caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc
    24121 tttctcctcc taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac
    24181 gtattaacat ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag
    24241 aaacttttga aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca
    24301 aacttaagaa gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac
    24361 gccatctaga taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc
    24421 gcgtccttga gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag
    24481 actagccatc atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat
    24541 tgcttctttc aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc
    24601 ttcttttact actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc
    24661 catcatacag tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc
    24721 cgcagcattt ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa
    24781 agataataac gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa
    24841 agcatggaca aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc
    24901 attactgtgt cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc
    24961 tacaccttga cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg
    25021 ccattcatgg aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt
    25081 gtgttcttct gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc
    25141 ttcctgatac attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt
    25201 caatgttgtt ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt
    25261 gatgccttct gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc
    25321 taccaaacca ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg
    25381 tagttctttg gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt
    25441 gacacctgac gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc
    25501 ctttgttttt ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac
    25561 catttacaaa ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc
    25621 tctttctggt tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag
    25681 gtaatagtag ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc
    25741 ttcgtcgatt tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa
    25801 ttcacttttt gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata
    25861 gtagcgtgct tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag
    25921 ttgtatttct gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc
    25981 tttcaaataa taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag
    26041 ataataataa tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga
    26101 ttttagtact tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc
    26161 aaataattta tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt
    26221 tacccctagc cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt
    26281 ttcgatccga cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat
    26341 tttttgttct tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat
    26401 aagtatcctc cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg
    26461 caaacttcaa gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca
    26521 ctagaaagag agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta
    26581 aaatcatttt tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa
    26641 atcatccatt ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc
    26701 accaccatgt acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc
    26761 atccaaaata ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg
    26821 ttttacggct gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc
    26881 atagatcctt ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt
    26941 ttggataatt tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag
    27001 cccttctata tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg
    27061 aaatcctgct acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg
    27121 cgctaatgta gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc
    27181 gtcggaataa ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc
    27241 ttttggataa agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg
    27301 actcgttatt tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca
    27361 aatataatga acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc
    27421 cggcattgga accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat
    27481 caattcgctc actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt
    27541 cagttggcaa tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta
    27601 gccttctgag tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa
    27661 tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat
    27721 ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt
    27781 ggatttttca aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc
    27841 caaatttttt gttatttact atttcaatat catactccat cctagaaaat tttgccactt
    27901 atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat
    27961 gatagtcatt ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca
    28021 taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca
    28081 gtcctgaaaa ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg
    28141 acaactatgt aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt
    28201 ggcagacaag catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc
    28261 atctgcacta caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc
    28321 tttatgccat tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa
    28381 cagcctgagg tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt
    28441 aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt
    28501 tgtatattat ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc
    28561 aaattgcata aattcatttt tttgtacact catgcttttc attagctctt taactgccat
    28621 tagtactaag atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca
    28681 tactgtggga gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac
    28741 taaaaaaata gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag
    28801 taataatgcc aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt
    28861 cgttcctaac tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt
    28921 atttccattg acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt
    28981 taagaaaaac ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt
    29041 tagaaggaaa attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg
    29101 ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc
    29161 aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag ttg
//
LOCUS       DANKHK010000026        28192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000026 DANKHK010000000
VERSION     DANKHK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            90..1055
                     /locus_tag="PW109_001066"
     CDS             90..1055
                     /locus_tag="PW109_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2510928.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1112..1342)
                     /locus_tag="PW109_001067"
     CDS             complement(1112..1342)
                     /locus_tag="PW109_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2510929.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1428..2315)
                     /locus_tag="PW109_001068"
     CDS             complement(1428..2315)
                     /locus_tag="PW109_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2510930.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2404..3627)
                     /locus_tag="PW109_001069"
     CDS             complement(2404..3627)
                     /locus_tag="PW109_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2510931.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3644..4690)
                     /locus_tag="PW109_001070"
     CDS             complement(3644..4690)
                     /locus_tag="PW109_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2510932.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4854..6581)
                     /gene="ptsP"
                     /locus_tag="PW109_001071"
     CDS             complement(4854..6581)
                     /gene="ptsP"
                     /locus_tag="PW109_001071"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2510933.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6581..6847)
                     /locus_tag="PW109_001072"
     CDS             complement(6581..6847)
                     /locus_tag="PW109_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2510934.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7326..9560
                     /locus_tag="PW109_001073"
     CDS             7326..9560
                     /locus_tag="PW109_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2510935.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9708..10028
                     /locus_tag="PW109_001074"
     CDS             9708..10028
                     /locus_tag="PW109_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2510936.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10233..10457)
                     /locus_tag="PW109_001075"
     CDS             complement(10233..10457)
                     /locus_tag="PW109_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2510937.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10655..12235)
                     /locus_tag="PW109_001076"
     CDS             complement(10655..12235)
                     /locus_tag="PW109_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2510938.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12636..14012)
                     /locus_tag="PW109_001077"
     CDS             complement(12636..14012)
                     /locus_tag="PW109_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2510939.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14140..15258)
                     /locus_tag="PW109_001078"
     CDS             complement(14140..15258)
                     /locus_tag="PW109_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2510940.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15400..15834
                     /locus_tag="PW109_001079"
     CDS             15400..15834
                     /locus_tag="PW109_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510941.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15912..16445)
                     /locus_tag="PW109_001080"
     CDS             complement(15912..16445)
                     /locus_tag="PW109_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2510942.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16539..17717)
                     /gene="mutY"
                     /locus_tag="PW109_001081"
     CDS             complement(16539..17717)
                     /gene="mutY"
                     /locus_tag="PW109_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2510943.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17710..18507)
                     /gene="recX"
                     /locus_tag="PW109_001082"
     CDS             complement(17710..18507)
                     /gene="recX"
                     /locus_tag="PW109_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2510944.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18614..19996
                     /gene="rlmD"
                     /locus_tag="PW109_001083"
     CDS             18614..19996
                     /gene="rlmD"
                     /locus_tag="PW109_001083"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2510945.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20170..20748)
                     /locus_tag="PW109_001084"
     CDS             complement(20170..20748)
                     /locus_tag="PW109_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2510946.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21070..21540
                     /locus_tag="PW109_001085"
     CDS             21070..21540
                     /locus_tag="PW109_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2510947.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21648..22874)
                     /locus_tag="PW109_001086"
     CDS             complement(21648..22874)
                     /locus_tag="PW109_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2510948.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23628..23828)
                     /locus_tag="PW109_001087"
     CDS             complement(23628..23828)
                     /locus_tag="PW109_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2510949.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23962..24570)
                     /locus_tag="PW109_001088"
     CDS             complement(23962..24570)
                     /locus_tag="PW109_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2510950.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24655..25125)
                     /locus_tag="PW109_001089"
     CDS             complement(24655..25125)
                     /locus_tag="PW109_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2510951.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25173..26087)
                     /locus_tag="PW109_001090"
     CDS             complement(25173..26087)
                     /locus_tag="PW109_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2510952.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26233..27036
                     /locus_tag="PW109_001091"
     CDS             26233..27036
                     /locus_tag="PW109_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2510953.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27214..28161
                     /locus_tag="PW109_001092"
     CDS             27214..28161
                     /locus_tag="PW109_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2510954.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         8496 a         5668 c         4748 g         9280 t
ORIGIN      
        1 tgtgaacaga actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc
       61 tttttattca aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca
      121 tgctgccggg tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag
      181 caaaacggct caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa
      241 aagtattacg tatgattcat catataacag attttgattt cgtcgtagta gacacggaat
      301 tagatgcttt attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac
      361 aaatgaatta tttcagtaat tataattatg tacatattac caataaggga tttgtattaa
      421 cagatactcc tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta
      481 ttttgcgtat aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag
      541 ctttgcgagt ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac
      601 tccaagggct gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac
      661 aacatttcat ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat
      721 tagttactta ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc
      781 tcaccttcag catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt
      841 atggtcaact tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg
      901 aaaagaaaga agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg
      961 tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt
     1021 taaacaaaaa agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat
     1081 cttcaacttc aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg
     1141 ctacgatatc gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa
     1201 atgtatcggt cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac
     1261 ggcgaacttg cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg
     1321 cgactaattg tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc
     1381 aaaagcatag cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt
     1441 tctggtattt ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca
     1501 aaacctttat ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca
     1561 agatccattt tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca
     1621 aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag
     1681 ttagctgcac ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt
     1741 tgtgcataga caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta
     1801 gccatttttg tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat
     1861 tctttgaata aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc
     1921 agtgtaccat tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg
     1981 cctttttctt ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa
     2041 tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt
     2101 tcttctacat ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc
     2161 gcttttggtg tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt
     2221 gtccgattat agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt
     2281 cccatgacgc ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa
     2341 atccttgctt ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga
     2401 attttatctt ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc
     2461 ttttcttcgc atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc
     2521 ttcgccgaaa tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt
     2581 aggaacttca ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa
     2641 agttttccct aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac
     2701 acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga
     2761 agcactgaca aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc
     2821 tcctgtgaac tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc
     2881 tacataaggt ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg
     2941 aggaaaccca ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag
     3001 aatcatcaaa tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg
     3061 agtgatatca gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg
     3121 tgctattaca ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc
     3181 tgtcgtatga tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt
     3241 tcctttaccg atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg
     3301 tattttcaac ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt
     3361 atgaataaga tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac
     3421 aacaactctt cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt
     3481 ttccaactta tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct
     3541 ttctaattct tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta
     3601 ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat
     3661 ttactttttt taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa
     3721 gttcttcacc gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg
     3781 tactcggtag ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct
     3841 tccccatata gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa
     3901 caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat
     3961 aagaagggaa aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa
     4021 tgtaaccagg aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc
     4081 gaggaatcga gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct
     4141 tagcaatttc tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga
     4201 ttactttttg atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat
     4261 cagggtgata tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac
     4321 catagctttc caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt
     4381 ggtacaatcc taccagatat ttttttacga taggagaaag ctggttcgat ccgataaagg
     4441 aatttcggaa atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc
     4501 tggctttatt gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat
     4561 aatcttcttt ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat
     4621 gatcaagcgc acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata
     4681 ataccttcac cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac
     4741 tcaaacttaa gccctatttt tgtctattct tactgacaaa aacgttttct taaaaaagaa
     4801 caaaaaaact ggcgtagctc ttttacaagc cacaccagct tttgatacgt tgtttatttt
     4861 acatattcat gaacaagttc aacgacttct tccatcatat cgcattcttt caatgcacga
     4921 tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca ttaaactacg cgttttaaga
     4981 atagatgtcg cactcattga gaactcatct agacccatac ctactagcaa tggaacagct
     5041 gtttgatctc cagccatttc tccacacata cctgcccatt ttccttcagc gtgtgctgca
     5101 tcaattacat ttttgatcaa acgtaaaatt gatgggttat atggttggta caagtaagaa
     5161 acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa tcaaatcgtt cgtacctaca
     5221 gagaagaaat ctacttcttt tgcaaacttg tctgctaaga ctgctgctgc tggaatctcg
     5281 atcatgatgc cgacttggat cgtatcagat acttccttgc cttcacttat caatttttgt
     5341 ttttcttctt caaagatcgc ttttgctgcg cggaattctt tgagtgtagc taccataggg
     5401 aacatgatac gtaaattacc atgaacagat gcacgaagca atgcacgcat ttgtgtacgg
     5461 aacatttcgt cgccttgttc agataaactg atacgcaatg cacgatatcc taagaatgga
     5521 ttcatttcat gaggtaattg cagatatggt agttctttat ctccaccgat gtccattgta
     5581 cgaacaacta caggttttcc ttccatacct tctaatacag ctttgtaagc ttcatattgg
     5641 tcatcttctg ttgggaagtc tggagaatcc atatacagga attcagtacg gtacagacca
     5701 actgcttctc caccattatt atggacacca actagatctt ttggtgttcc gatgtttgca
     5761 gctaattcaa aatgcttgcc gtcagctgtg acagtttctg cattttttaa tttttcccat
     5821 tctgcttttt gtgctgcgta atctgcacct gctttttcaa attcagcttt ttgttcatct
     5881 gtaggatcga tgattacatc gccttcgatc ccgttgacag ccaaaatatc gccttctttg
     5941 actttagcag taatttcttt tgtcccaacg attgctggaa tttctaaaga acgtgccatg
     6001 atagcagaat gtgaagtacg tccaccaatg tctgttacaa acgcttttac aaaattgcga
     6061 tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa caacgaccac ttcttcgttg
     6121 atcattgatg gatttggtaa agtaacgcct aataagtgcg ctaaaatacg tttagcaacg
     6181 tcacgaatat ctgcagcacg ctcttgcata taagcattat cttccattgc ttcgaacata
     6241 ccgatataca tatccgttac ttcttttagc gctgattcag catttacgct gttgtcttta
     6301 atattttgct tgatttgacc taccatttct ggatctgaaa gaaccatcaa atgggcatca
     6361 aatacttgcg cttctgcttc acctaagctt tgcgctgctt tttcacgaat ttgctgcaat
     6421 tcttcagtag atttagctaa tgcagcatcc aaacgagctt cttcagccgc agtatcttct
     6481 actgttgcct tactgaatgt taaatccggt tgaactagca ggtaagcttt tgcaacggct
     6541 acaccgtcac tagcggcaat cccttttagc atttcaacca ttattcagat aagccttctt
     6601 tcttcattgt ttcaacgatt gctgccattg catcagcttc gtcagcacct tctgcagtaa
     6661 tagtaacatc agaaccttga cctacaccta aagacataac gcccatgatt gatttcaagt
     6721 ttactgattt acctttatac tctaagttta catcagagtt aaatttgctt gctgtttgta
     6781 ctaatagtgt agctggacga gcgtggatcc cagtttctgc tactacgtga aattctttct
     6841 tttccatatt aatcggtctc ctttaagtga gtgttgtttt tatgtgaggg ttttcttttt
     6901 tagtaattca gagaatccta acaaatgcca ccctttcatc tgtaaagatt aacatttttt
     6961 ttcgttagat acaacagttt tcattcaatt tcagataaaa gtattcaaat tattccggat
     7021 tctttttcat ccttttcttc acattttata acaaacgtgt ttatttcctt tttcttgtcg
     7081 ctttctcttc tcctgacgtt tatcattctt tcctttgacc ctctttgttt tcatatgaaa
     7141 cgaattactt gcttcccttt ttatttttag tataataaga gtcaaaggtc aggaatggtc
     7201 aatagagatt tcaccttttc tacctcataa atctcttaaa aaactagggg ttattctttt
     7261 acctcatgaa aaacgagatg taaaatatgg atgtacccat ttttgtgaaa ggacgtgaat
     7321 gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac gattcacttg tatgccactg
     7381 tcaatggtca gcggacacaa ctcgattact gtcagagctg ttatcaaaaa atcaaaaatc
     7441 aacaaaatgg aggtctgact aatatggccc aaaatgatcc atttggattt ggcagtttgg
     7501 acgatctttt ccgttccatg tctcgccaaa tgcaacagca aggaaattat gagcaaacac
     7561 caccaacaca atttggcgga aacaacaact tcaatggtgg tcagccacct caaggcaata
     7621 cagaaggttt attgggagaa tacggaatca atatcacaga acaagcacga caaggtgata
     7681 tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt cattgaaatt ttaaatcgcc
     7741 gaaccaagaa caatcctgtc ttgattggag aaccaggtgt tggtaaaaca gcagttgttg
     7801 aaggtttagc acaaaaaatc gtagatggtg atgtaccgca aaaattaatg gacaaagaag
     7861 tcattcgttt agatgtcgta tctttagtcc aaggtactgg tatacgagga caatttgaag
     7921 aacgtatgca aaaattgatt gaagagatcc gtcaagcaga aaatgtgatc ctattcatag
     7981 atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga tggaaatatg gatgctggta
     8041 atattttaaa accagcttta gctcgaggag aattgcaaat ggttggtgct actaccttaa
     8101 atgagtaccg catcatcgaa aaagacgctg ctttagaacg tcgtatgcaa cctgtacggg
     8161 tagatgaacc aactgtcgaa gaaacgatca gtattttgaa aggtttgcaa aaacgttatg
     8221 aagactatca tcatgtaaaa tatactgatg aggccatcga agcagccgca actttatcca
     8281 atcgttacgt ccaagatcgc ttcttgccgg ataaagccat cgatttgcta gatgaaacag
     8341 gttctaagaa aaacttgacg atccaaatcg ttgatcctaa aacaatcgag aaaaaactgc
     8401 aagaagcaga agaacaaaaa gttttagctt ctcgagaaga agatttcgaa aaagcagctt
     8461 attatcgcga tcagatcaat aaacttcaaa aaatgaaaga acgtcagctc actgaagagg
     8521 aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt tgaacaacgg acaggtattc
     8581 cagtgggtga attaaaagaa aaagaacaga cacaactgaa aaacttagca gatgacttga
     8641 aagctcatgt catcggacaa gataacgctg tagatcgagt agcgaaagcg attcgccgaa
     8701 accgtgttgg attaaacaaa cagaatcgtc caatcggctc cttcttattt gttggaccaa
     8761 ctggtgtagg taaaacagag cttgcgaaac aacttgccta cgaactattc ggttcacaag
     8821 attcaatgat tcgtttcgat atgtctgaat atatggaaaa acatagtgtc tctaaattga
     8881 ttggttctcc tccaggttat gtcggatacg aagaagctgg tcaattaaca gaaaaagtac
     8941 ggagaaatcc ttacagtctt gtcttgttag atgaagtaga aaaagctcat ccagatgttt
     9001 tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac agatgcacaa ggaagaacgg
     9061 tcagctttaa agatacgatc atcattatga caagtaatgc cggaactggt aaagtagaag
     9121 caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg ttctgtattg aaccaattaa
     9181 acaactactt tacaccagaa tttctgaacc gttttgacgg gatcatcgaa ttcagtgcat
     9241 tatcaaaaga aaacttgatg acaatcgtca cactaatgct agacgatgtc aaccaaatgc
     9301 tggcagcaca gcaacttcac atcgaagttc ctacgaatgt caaagaaaaa ttagttgact
     9361 taggctatga tccatctatg ggtgctcgtc ctcttcgacg cacgatccaa gaacaaatcg
     9421 aagatggtat cgctgaattt tatcttgatc acccttctat ccatgagttg aaagcaaaac
     9481 tggataaaga tggaaaaatc atcgttactt ctaaaccaga acgtttggct aaagaatcag
     9541 ccgaagaaac ggctgaataa atccagtttt atagcgtgag acggtatttc tttgagaaat
     9601 gccgtctcat tcttttttca gaaaagtcaa aatactgttc attttataaa aaagaaatta
     9661 taataagaga aacaagcaat taaaaataca taaaagaggt gagcgccatg aaattggtaa
     9721 atgtaacaaa cagccattcc cgtcttgtat taaaccagct tgaaaacact gatgcccatc
     9781 tagtcaaggt ctacacagcc ggaaatacca ctattgtcta cactgaagcc cctgaacata
     9841 atgaaattct gctgatcaat gacaaacgta aaatccagcc gaaagaaatc gaagatgcta
     9901 aaaattattt cttagataaa atcaaagacg ccgtttacca cgaggatgaa atcaaagtaa
     9961 tcgaattggc aggccttgtt gaaatctcga ttccaaaaca tgaattaggc gctacattag
    10021 ccgtgtaaac aaattttgaa cataataatt aaagagcgtg ggacaaaagt aaagaacact
    10081 tttgtctcac gctctaaaca cgtataaacg gcgaaagcag aagcaactcc ttcggaaata
    10141 agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa ttccagctta tttcttggag
    10201 ttaaacgctt ctgtctcagc ctctttttct ttctagcttt ttgatttttc tcttttcttt
    10261 tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt tatttttaac tttttcctct
    10321 ttatggatga ttgatcgtac tttttgtgca atttgctcgt ttttgtccca gtcttttaca
    10381 tctaataatc gacataatcg taacatctct tcttttttct gtttttttct ttcacgtctt
    10441 tgccattctg tttccatgtg tcacactctc ctttctttaa tcatatgtac gcaaaataat
    10501 ttttatattt ttttgattag acatttctat gattttaata tagtaaaaag agccgtcacg
    10561 gaaaatccgt cacggctcta aacaaaatat gagaggctgt ttttgtttgg ctattcttat
    10621 tacttgatgc agggcagctt gtgtcaagac ttctttacat caaacttttt aattttacat
    10681 tgggatattt atccgcaaac cagcgttctg caaattggtt ctcgaataag aacaacggtt
    10741 ggtcgaaacg gtcacgagcc aagatattgc gacttgaact catcttctca tccaaatctt
    10801 ctggatcgat ccagcgagcg attttgtgtc ccattggaga catgattact tcggcgttat
    10861 actcatttaa catacgatat tggaatactt cgaattgtag ctgaccaaca gctccaatga
    10921 tatattcttc cgttagatat gttttataaa gctgaatcgc tccttcttga actaattgat
    10981 agattccttt gtggaaggat ttttgtttca tgacattttt tgcagtcact ttcataaaca
    11041 gctctggagt aaatgaaggg agttcttcat aagcaacttt tagttttcct tcgaacaatg
    11101 tatccccaat ctgataattg cctgtatcat atacaccgat gatatcgcct gctactgctt
    11161 tttcgacgtt ttctctcgaa tctgccataa attgtgtgac attgcttaat ttcagttttt
    11221 tatttgtacg ttccaaccag acatccattc ctcgttcgaa tgttccagaa cagattcgga
    11281 caaaagcgat tcggtcacga tgggctggat tcatatttgc ttggatcttg aatacgaaac
    11341 ctgaaaattc ttcctcataa ggacttacca tctgatcatc ttgtgttttg tgtccataag
    11401 gagctggtgc caattcaacg aaagtctcta aaaaagtctg tacaccgaag ttagtcaatg
    11461 cagaaccaaa aaatactggt gtctgatcgc cacgagcaat tttttcttga ttaaatgcat
    11521 ctccagcctc tttgattagt tctacttctt ctaagacttg ttcatataag gaattttcag
    11581 ttagaggatg attttcggga agcagtccat cttcatccaa ttctactaat cgttcatcct
    11641 gataattttc cggacgataa aactctacac gattatggta gatatcataa agaccttcta
    11701 gccctttacc cataccgatt ggccaattca tcggataaga ttcgatatct aatagctctt
    11761 ctaattcttc taacaattcc aatggttcac gaccatcacg atctagtttg ttgataaaag
    11821 taaatatcgg gatgccacgt ttttttacaa cttggaataa tttctttgtc tgtgcttcga
    11881 tcccttttgc actatcgatt accatgaccg cactatccac ggccatcaaa gtacgataag
    11941 tatcttcaga aaaatcttca tgtcctggtg tatccaagat atttactcgt ttcccttgat
    12001 aatcaaactg cataacagaa ctagtaacag aaatcccacg ttgtttctcg atttccatcc
    12061 agtctgattt ggcaaaatta cctgtttttt tgccttttac ggtcccggct tgacggatag
    12121 ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt cccagcatcc ggatgggaaa
    12181 tgatcgcaaa agttctgcga ttgtccactt gttgctttaa ttttggatta ttcataacct
    12241 aactccattt cttccattca attatttata ttttctctag gcacagattg tccgatctct
    12301 taaagaaaga caatttcata tagggctgtg acaaaagccg tgtagcagtc tctttttccg
    12361 agtaaacggc gttctagaga cagcctatca gcaataagcc cacccaagcc aaaaatacga
    12421 aaagctgttt ttgcttggct gctcttatta cttaatgcag gatagctttt tcacaacctt
    12481 tgtcaactac tacaactgaa tcagtatagc atggattcag cgaatgaaaa agccaccaaa
    12541 ccataagagt caaatatttt tttgtcagct gactaaaaga aaagacgtgt tgcttgttta
    12601 ttcaagcaac acgtctttca taaccaagtt tccaattatc ttcttggctc gtcatcttcg
    12661 aattcttttc tgaagtatct tcgttcttct tcaggttccg cttctgtttc ttccggatca
    12721 tgaaaaatga cacggatttt aaggattcga gaaccttcca tttcttcaga aaccagcgta
    12781 atattatcga catcaaacga taacttttcg ccttcatccg gaatcatgcc taatgctgta
    12841 atcaaataac cagccatcgt atctacatca ctcatatgca gatcactgtc aaaagcttca
    12901 ttgaactcat caatcagcat acgtccttgg atgatatatt cgtgttcatc gattttttca
    12961 taaagattct caacttcatc tgattcatca tcgatttctc cgacgatttc ttctaaaagg
    13021 tcttctagtg ttgctaaccc aacgacgccg ccatattcat ctaataaaat agccatctga
    13081 ttctgcgtct ttttcatttc atataacaga tcatcaataa agaccgtttc aggaacgaat
    13141 aaaggctcct gcatgatttt cttgatatct aaattatcaa agccgaattt atgggcagct
    13201 tttagtaaat tctttgtatg aaggattccg actactttat ctttgtcttc gttataaaca
    13261 gggattcttg aatagttttc ggataaaact tcattcacat tttcttccac agtgtcgttg
    13321 atatcaatca taaatgcatc cgttcgtggg accatgactt ctcgggcaac tttcgtatca
    13381 agagaaaaaa ccccttggag catttctaat tcttcattat tcaatacgcc ttcgttttca
    13441 agcatataac gcatttcatc tctggtcatt tttgaatctt catcatcaaa agtcatcggc
    13501 gtgatacgcg ccaataaact tgtggaagca gaaagcagcc agacgaaggg cttagctact
    13561 acaccaatca agcgtatcag ccctgaagtg aactttgcga cttcttctga tttatttaaa
    13621 gcaatacgtt tagggtacaa ttctccaaaa acaatcgata cataggtcaa aatagccagc
    13681 acaatgatat ttgcaatatt ttttgccgct gtgccgccac ctaataccgg agctaaccgt
    13741 tccgataaag tattagctag tgaagcacca gacaaaatgt tgaccaaagt gattcctact
    13801 tggattgtcg ataggaaatt atttggattc tgcaatattt ttaaaagctt ttgagatttc
    13861 acatctcctt catcagcttt ttgctcgatt cgattcttat tgatcgaaac aacagcaatt
    13921 tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga tcaataataa aatctgcgct
    13981 aacagcgact gactctcagg gtcagcattc ataatcgcat tctctcctta taaaattaat
    14041 ttttagttta cgatagaaaa aaacgtggca ctatagccac gttaaaatgg tatcacaaag
    14101 caattttctt tgcaataaaa acaagtgtac taaaactacc tacgaggtcg tagcaatttt
    14161 cttcttgttt tcattttgct tgtcttcttt gacgattttg attatatagg aaatgatcgt
    14221 cttgcagatt gcgtaaaaag gaacacctaa gaagatacca agcaatccgg caatatttcc
    14281 agctactaga agaacgataa tgatcgttaa tggatggatt ttcagtgatt tcccaattac
    14341 attaggataa atgatattcc catccaactg ttggacgatt agcacaacca cacaacaaag
    14401 taatgctttt accggatcat tgaagactgt tactagaaat gcaggtgcta gtcctagata
    14461 aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg accccaaata agaaagcata
    14521 atccacacca attaccatat atcctaaaaa tgtgaaggta ccaacgaata aacattcgat
    14581 tgcttgtccg ctaatgtaat tagccaatgt tttattcagt tgccccaata actccacaat
    14641 ttggtttctt cttttttctg ggaaaaactg ttggatgttt ggcactagtt tttctccgtc
    14701 tttcaacata tagaacaaga tgaatggtgc tgtaatgatt actacggttg ccgaagcgat
    14761 cgttccaacg atcgaaccta aactattgga tactccacta aggaattgct gaatgattgt
    14821 cccgtaagaa atgtctaatt gctcaaaata tttattgata tctacttcct tgaacaaagg
    14881 caattcggct acctctttta accagctttc tacctgtttg atgaaatcag gcatattaga
    14941 tgccaatgag gaaatttgct gtactagact tgggatgaca ctaagtaaaa tccaaataat
    15001 tgctgcaact aaaaggagca gcacaattgc gacagcccat attcttttga tttttgcttt
    15061 catcaacagc ttgacaatag gattcaataa atagtaaaga aagcctgcaa tcagtacagg
    15121 tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa ataaagttta tttctgatga
    15181 aatcatgatc aatgcagcta tgatcaataa ttcaagcgac caaaacatta gtttagactg
    15241 tctaagtttt tcaaacaata gatttcctcc tttctttctt actagtatat attatattcg
    15301 ttaaaaattc ttttattctt tttttatttt tgaaaacttg ccatactttt tatttgtttt
    15361 attttccagt atactatacg aaaggaaggt ggctagacca tgaaagttgt acaaacaaaa
    15421 gatacaatga gcaatattta cttagatgct gtacggatca ggcatcaagt ttttgtagtc
    15481 gaacagggtg tgcctttgtc tcgagaaata gacaaagatg aagcccattg tatccacttc
    15541 gtcctgtact cagataaaaa agaaccgcaa ggaactgtac gtcttttgcc tttagaaaac
    15601 ggaaaaatga aattacagcg gatggcgatt ctttctgaat acagacacca agggttagga
    15661 aaaatattga ttgaagaagc agaaaacttt gctaagaatc aaggctataa tacgatttta
    15721 ttaggggcac agtccacagc tgaaactttt tacgaaaaac taggatatac agcttacggc
    15781 gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt ataaaaattt
    15841 atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg gcttactgtt
    15901 cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat atcaatatag
    15961 ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt cacattttct
    16021 tttaggatga agtcaatatc gttcgggtaa ttcatcattt tgctgtgcat ctcatattca
    16081 tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg
    16141 atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca ataataggaa
    16201 accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg aaaataaaca
    16261 atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca
    16321 ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg ccacgttcga
    16381 tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc ttttggaact
    16441 cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt tcattacacc
    16501 gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt cctattgttt
    16561 cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac gaccgaatca
    16621 aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg ttctgctccc
    16681 tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa aatatgagta
    16741 acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa tgggagattt
    16801 tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt ttcccattct
    16861 tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt ccacatattc
    16921 gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg attttgaaga
    16981 gctgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac aggaaaactc
    17041 gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt ctcacacttt
    17101 ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt gaattcacct
    17161 ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct gctagaagct
    17221 tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca
    17281 actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct
    17341 attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc aaattctgac
    17401 atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc aaggccctcc
    17461 catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt aggaaaccat
    17521 tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg aagcatgatc
    17581 tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt tcttttttct
    17641 tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc tgtccatttt
    17701 tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga tcatatccaa
    17761 atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt taggttcaaa
    17821 ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat attctatttc
    17881 ttgatctgct tcttgcggaa gatcagctat cgcttgttgg atcacttctt gagaaaagcc
    17941 tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt ttccgtgatt
    18001 ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt caaatgtata
    18061 ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca tctgctgcgc
    18121 taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac tttccccata
    18181 gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat ttcgatcttc
    18241 ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc gcaactgata
    18301 acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct tcttgatctc
    18361 ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta ctaatacatc
    18421 ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag ggcctttgcc
    18481 tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat tctctatcat
    18541 accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag aaaaaagaaa
    18601 aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc ctttgaaaat
    18661 caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga tcatatttgt
    18721 tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta
    18781 tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg
    18841 tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc aagctcaatt
    18901 ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag caggctttcg
    18961 atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata gaaacaaagc
    19021 acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt tatacgaagc
    19081 aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc gaacacaaaa
    19141 agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct atgatgaacg
    19201 gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt
    19261 acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa tgatccgtga
    19321 gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac
    19381 gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca ttgaagaaca
    19441 aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca atcctcagca
    19501 gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta atgaaacgat
    19561 cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac aagccaaaga
    19621 agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag
    19681 attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc tgcctaaatg
    19741 gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta cgggtttgga
    19801 taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt atatctcctg
    19861 taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca agattgaata
    19921 tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac
    19981 gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc aagtaataag
    20041 aacagccaaa caaaaatagc tcttcatgtt ttatgaatac tgtacttgtg accgaagagt
    20101 taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg tcacagccct
    20161 ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg tcgtatcgct
    20221 gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa gtgtgatcgt
    20281 ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga tcaattcaaa
    20341 atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt agtaatccca
    20401 agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc ctggattaac
    20461 catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc
    20521 tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac tttgcggttc
    20581 ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc ctaggtatgc
    20641 acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa
    20701 ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt agaaacctcc
    20761 ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt atgcttttat
    20821 aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa cagaagtaga
    20881 cgagaaaagt acaaatagaa aacatgagaa tcaaatcata tgatcttttt caaaaacatc
    20941 attcatataa acaaatgttc aactttttaa taacaacaat tatcaatttt tttcttactt
    21001 cacaaactaa atacaggtct tgctgtgagc cttcatggta aactaatatt atagaaagga
    21061 gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat aggaattgtc
    21121 atattaggct ttttatcagg gttgcttagc ggaaatcccg gaaaatatta ctattctttg
    21181 caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac cttactgtat
    21241 atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa acagcgaaac
    21301 aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc tgctctattt
    21361 tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt tattttcttg
    21421 tcagtgctta tgtatcagtt gtggctgcat catcgcctgg caatgtggct catgattcct
    21481 tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt tttaaactaa
    21541 gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct aagttagatt
    21601 ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta gtacccactt
    21661 gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg tccagcgtaa
    21721 ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc ttctgtttgc
    21781 tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata ggaagaataa
    21841 tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc ttccgaatca
    21901 tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc gcttgttttt
    21961 atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt tcctaaaatt
    22021 tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg tttgattgtt
    22081 tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc taagtccgtc
    22141 gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc atctaaactt
    22201 aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg accgatatcg
    22261 ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc attcaatgtt
    22321 tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt attcactttg
    22381 atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt tacataatga
    22441 tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac accaccaaat
    22501 gcgtaggcat gatttaattt atctaccgtt ccataaccta cgatattcgt ataaatatct
    22561 cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac taccatcatc
    22621 gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc taatagcaaa
    22681 accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga ggatattcga
    22741 ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa tctgattccg
    22801 tatgctgata atccggcaat cagcaaaacg aataccccta acaaggttaa tagaactttt
    22861 tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc tcatataact
    22921 gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat ttttgtgtaa
    22981 cgattattat tttttaaaat ttctcaaaat tcttaactat cataccatac cgtgaaaaag
    23041 atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttatg tattttttcc
    23101 agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga tcgcacatac
    23161 ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga tttcacgaca
    23221 atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt atttccagct
    23281 cactgcatgg ctcttaaaat gactcgaact agtgattgca ttaccatcat cttttaatgg
    23341 atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga attgacatgg
    23401 taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc caaaaactga
    23461 aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa ttagttagga
    23521 catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc ataaatagac
    23581 aaaaagacac gactctctaa gaagtcgtgt ctttattcaa caaattatta taatttagtc
    23641 acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac ttcttgacct
    23701 tcttctaggc tcttgaagcc gtctccttga attgctgaga aatgaacaaa tacgtcatct
    23761 ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt tgaaccattt aaccgttccg
    23821 tgttccataa atgtatatcc tccaaaaaag ttcttgcaag taaccttgca agaacattat
    23881 acccaattta gaaaacgcta gcaagctttc gctcttttat tttccaaatt atttttaaga
    23941 ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta cttcatccca
    24001 attaataaca ttccagaatg ctgcaatata atctggtcgg acatttttat atttcaaata
    24061 ataagcatgt tcccaaacgt ccaaacctag aattggtgtc tttccttcca tcaatggaga
    24121 atcttgattt gcagtagagg taatagctaa ttttccattt tccattacaa gccaagccca
    24181 tccagaacca aatcgtccag cggctgcttt tttgaattct tctttaaaag aagaaaaatc
    24241 accaaaagct tcattaatcg cttcttttat ttctcctgta ggttcgccac cagcatttgg
    24301 tgccatgatt tcccagaaaa atgaatggtt agcatgtccg ccaccattgt tacgtacagc
    24361 tgtcttgata tctgttggaa tagcgtccat atcagataat aattcttcta ttgttttttc
    24421 gcctaattct gggtatttct caattgctga atttaaattc gtcacataag tattgtgatg
    24481 cttatcatga tgcagatgca tcgtttcttc gtcaatataa ggttccaatg cgtcatatgc
    24541 ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg ttttcttaat
    24601 aatgtctaca ttacaaagag taacaaagag aaggaaattc ttcaattgtt atgcctagtc
    24661 gtctttttca ctttgaccta cttcttcttc aaaaacctca gtttctttaa aaataaacaa
    24721 cttgctgaaa atatagttcg caaggataac aataatttga gtgatgattt tcgccacata
    24781 ctcattgctg ttcataacat tgatcaacaa taccattgcc cccatgtcca atccgtaaga
    24841 aaggatacgg tagaagaaga acttaccgca ttcaataagt gcatgtgtaa aattcggtga
    24901 ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca aacaatacag aacaaaacca
    24961 cgaaatagaa ttggataagg ccatacttaa atcaaaatat tggcgtgcga gagcaaaact
    25021 aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga tagactagta tctcccaata
    25081 tccttttgct tctaaatatc cttttaactg tcgatactgc ttcatgagcc aacctacctt
    25141 attaaaaatt cttatttatc atactctata tttcaataac tttcatcttt atttaaaaaa
    25201 attaattttt tttgatcctt tgcctttgtc tctgcttgga tcgtcacatg tgtaatacca
    25261 tattttaatt ttaataaaga aattatacga ggatatatct tttcaatctc gcttagtagc
    25321 tgatcttcaa aatcaatatg tccgtcgaaa ataattatct tttcgctata ctgccaaata
    25381 tggacatgat gaacatcctt taccccatcg attgcttcaa tttcttgttc cattttttca
    25441 tagtcgatag caggtgcact ttgtatcaaa atagcggcag cttttttgat caccattatc
    25501 gcttctatca atatatatat cgaaatgatc agtgtaataa ctggatcgat ccagtagata
    25561 tcgaaaaaac ggatcaaaat tgctccgata atcacactta ccgaagacag tgtatcactt
    25621 aacaaatgca aataactaga ttttatattc aaactttcat ctgcttcttg tcgtaataat
    25681 aatactgaga acaaattcgc gagcaatcca ataactgcta ctgttagcat caaatgacta
    25741 tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa caaggataat
    25801 atgatcaata tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg gtagccatac
    25861 gtacgttttt tatttgcttc ttttcgggcc attttttgtg ccccgtaaga aaaaataatt
    25921 gccaatgtat cactaaggtt atggaaagca tctgaaacaa gagacaaact ccctgacata
    25981 atccctccaa taaattctga taaagtgatc accgcattca aaaccactac tccaaatagt
    26041 ttttttgatg atctctgtgg ctgtgtaggc actattcctt cctccatttt atttatattg
    26101 gatagtcttt agacaagaat aacatttttt tgatatttca ccaatcattc agctgtttcc
    26161 ttaaagcact tgtatttcac ttgatacttc gctagaattg caagtgatga cactactaga
    26221 tgaggagtac ttatgacaac aaaacaatta attatcagtt cctttatacg attcaaagac
    26281 ctaaaggagg cgagaaatgt cccattttgg aaagccattg tctatctttt agttttgagt
    26341 ataattatgg ctttgccaat ctcttttcaa gtttttcaag tcttaaacaa cattcaattg
    26401 gatgggcaaa agattgctac tcgcattcct gattttacaa tccaaaacgg gcagcttcaa
    26461 accgaagaaa aagaaggatt catttaccaa acaaactcca ttatcttcac atttgatcca
    26521 gagggaaaac gaacagaaca ggacatctct actgatttga tggggaattt tgttagtgtc
    26581 gggatgttaa aaaacaaact tatcatagct cttcctaata caggcacgac ttctgctttg
    26641 ttgaacaata accaattaga acttccctac accaatgaat ctttaaaaaa cttaacaggt
    26701 aagcagctcc gttctttttt aagtgaagca tctataccac tttggataaa agtcctgaca
    26761 tttatatttt ctatttatcc tagttttctg aatctgctga tcactttgct atttaccaac
    26821 attgctgctt ttctctatgc acgctttcga ctaacaaaag ctacttttct tgattgttta
    26881 aaaacattga tctattcaat ttcattgcct actataattg caacaatctt gatggtcttt
    26941 cttccttctt ttgacactag cgccttcatt gcttttgcag gaatctttat ttttgctcaa
    27001 gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa taaagacaac tcgttatttc
    27061 tacttcaata gaccctttta gaggttgtga catttttttg tcacagcccc ttttttattt
    27121 gaaaaatatt tctttctcaa aaaacaagca aggcgcttca aagttcttct tgtatgcgct
    27181 acacttagag taaagaaagg cgggaaatta actatgtcag aaagaatcat ttatgcagat
    27241 cgtccatttc gtgatgaatt tatcgaagaa atccatacta tagcgcctgg ttatatcttt
    27301 aaaacagaat tacaaccaga ggatctacaa aatgtagaga tcagtttggg ctggaactca
    27361 gactaccaag aaaaactatt agcctcttct accttgaaat gggtccaatc tatctcagct
    27421 ggtgttgatc agctacccct tgagactttt tcaaagaaac atattttact ttcaaatggt
    27481 agtggcatcc acagtgaatc gattacggag catgtcttag gaatcatcct tgggtattct
    27541 cgtggtcttt ttcaagcaca aaaggcacag agtgacatga aatggttagg ttcatcggtc
    27601 cattaccaat ctatccaagg gaaaaaaata ctaatcattg gaactggcca tattggaaaa
    27661 atgattgcaa aaaaatgcaa agcattagac atgaactgct atggaatcaa tacatccggt
    27721 catccagcag aaggaatgga aaagactttt tctttaaatc aactaaagga agttcttcct
    27781 gaagtagatt tcgtagtcaa tatcttacct ctaacggagg aaacaactgg gctctttgat
    27841 cagcatatgt ttgaacactt tgatccaaaa tctgtattca tcaatgttgg ccgtggtgct
    27901 tctgttaaga cacaagatct ggtgcaggct ttaaataacc agcaactttc ctttgcagca
    27961 ttagacgttt ttgaggaaga gcctcttcca caagatcatc ctctttggaa aatggacaat
    28021 gtactgatta cttcccacat tgcaggtttg acacctgatt ttcaaaagaa attaatggct
    28081 atttttttaa ccaatctgaa aagctacttt gccacccatg agcttcaaac caatcaagta
    28141 aaattgtctg caggatatta atagctgaaa acaatatttt caaaagaaaa aa
//
LOCUS       DANKHK010000027        25690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000027 DANKHK010000000
VERSION     DANKHK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            129..2189
                     /locus_tag="PW109_001093"
     CDS             129..2189
                     /locus_tag="PW109_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2510955.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATAQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHSLTDIKNGGSAQ
                     VSYVYEKDYSQFEGKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2272..2841
                     /locus_tag="PW109_001094"
     CDS             2272..2841
                     /locus_tag="PW109_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2510956.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3016..3822)
                     /locus_tag="PW109_001095"
     CDS             complement(3016..3822)
                     /locus_tag="PW109_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510957.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3960..5474
                     /locus_tag="PW109_001096"
     CDS             3960..5474
                     /locus_tag="PW109_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2510958.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5877..6860)
                     /locus_tag="PW109_001097"
     CDS             complement(5877..6860)
                     /locus_tag="PW109_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2510959.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6865..7512)
                     /locus_tag="PW109_001098"
     CDS             complement(6865..7512)
                     /locus_tag="PW109_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2510960.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7597..8229)
                     /locus_tag="PW109_001099"
     CDS             complement(7597..8229)
                     /locus_tag="PW109_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2510961.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8245..8796)
                     /gene="coaC"
                     /locus_tag="PW109_001100"
     CDS             complement(8245..8796)
                     /gene="coaC"
                     /locus_tag="PW109_001100"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2510962.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8793..9530)
                     /locus_tag="PW109_001101"
     CDS             complement(8793..9530)
                     /locus_tag="PW109_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2510963.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9527..10516)
                     /locus_tag="PW109_001102"
     CDS             complement(9527..10516)
                     /locus_tag="PW109_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2510964.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10774..11316
                     /locus_tag="PW109_001103"
     CDS             10774..11316
                     /locus_tag="PW109_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2510965.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11333..12418
                     /locus_tag="PW109_001104"
     CDS             11333..12418
                     /locus_tag="PW109_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2510966.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12430..13236
                     /locus_tag="PW109_001105"
     CDS             12430..13236
                     /locus_tag="PW109_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510967.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13237..14064
                     /locus_tag="PW109_001106"
     CDS             13237..14064
                     /locus_tag="PW109_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2510968.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14061..15134
                     /locus_tag="PW109_001107"
     CDS             14061..15134
                     /locus_tag="PW109_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2510969.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSEYLGIYNDLFLEFKMYRR"
     gene            15562..18258
                     /locus_tag="PW109_001108"
     CDS             15562..18258
                     /locus_tag="PW109_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2510970.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18335..18823
                     /locus_tag="PW109_001109"
     CDS             18335..18823
                     /locus_tag="PW109_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2510971.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18835..19329
                     /locus_tag="PW109_001110"
     CDS             18835..19329
                     /locus_tag="PW109_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2510972.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19350..20159
                     /locus_tag="PW109_001111"
     CDS             19350..20159
                     /locus_tag="PW109_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2510973.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20156..20977
                     /locus_tag="PW109_001112"
     CDS             20156..20977
                     /locus_tag="PW109_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2510974.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21028..23127
                     /locus_tag="PW109_001113"
     CDS             21028..23127
                     /locus_tag="PW109_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2510975.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23124..24242
                     /locus_tag="PW109_001114"
     CDS             23124..24242
                     /locus_tag="PW109_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2510976.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24281..25159
                     /locus_tag="PW109_001115"
     CDS             24281..25159
                     /locus_tag="PW109_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2510977.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25350..25502
                     /locus_tag="PW109_001116"
     CDS             25350..25502
                     /locus_tag="PW109_001116"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2510978.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8125 a         4469 c         5427 g         7669 t
ORIGIN      
        1 aacaataaaa aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta
       61 tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact
      121 aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag
      181 ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga
      241 tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacggca caaaccatca
      301 aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt
      361 tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt
      421 atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa
      481 ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc
      541 agttaaaaca gatcactgat attttgaatc attctttgac agatataaag aacggtggaa
      601 gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaagga aagaaaatcg
      661 atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag
      721 atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga
      781 ctaatacagg aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa
      841 tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc
      901 ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa
      961 gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa
     1021 ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag
     1081 aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt
     1141 ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc
     1201 gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat
     1261 cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat
     1321 tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata
     1381 ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt
     1441 cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga
     1501 tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa
     1561 tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta
     1621 tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg
     1681 ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt
     1741 cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa
     1801 gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc
     1861 aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa
     1921 taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag
     1981 atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc
     2041 tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt
     2101 cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa
     2161 atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag
     2221 tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat
     2281 aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc
     2341 acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg
     2401 gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt
     2461 caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca
     2521 aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg
     2581 tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa
     2641 gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat
     2701 cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact
     2761 tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt
     2821 ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa
     2881 cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat aactaatggc
     2941 atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt
     3001 gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa
     3061 tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat
     3121 aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg
     3181 gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat
     3241 atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg
     3301 gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa
     3361 ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg
     3421 agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc
     3481 cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata
     3541 aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc
     3601 tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa
     3661 aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac
     3721 tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc
     3781 agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt
     3841 atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac
     3901 agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa
     3961 tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg
     4021 gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat
     4081 catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg
     4141 tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg
     4201 ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat
     4261 caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga
     4321 atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc
     4381 ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca
     4441 aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg
     4501 atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg
     4561 aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca
     4621 atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg
     4681 tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt
     4741 tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg
     4801 aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc
     4861 aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc
     4921 aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta
     4981 cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca
     5041 cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat
     5101 tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata
     5161 cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact
     5221 tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg
     5281 gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt
     5341 tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt
     5401 cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt
     5461 cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct
     5521 aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct
     5581 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga
     5641 cttattaccg cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa
     5701 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa
     5761 ttccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
     5821 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat
     5881 tcagttatct tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct
     5941 tgtttgactg cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct
     6001 agaaaaggcc cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt
     6061 ctaggtgtca ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct
     6121 atctcaggca cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct
     6181 atagaagcta aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact
     6241 ccccgacctt cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc
     6301 aattcatctg caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg
     6361 atgattttcg cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt
     6421 gctcctgcat ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt
     6481 tgataagcag tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca
     6541 gggagaagga ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg
     6601 actaatccgt atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact
     6661 acctctccag ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct
     6721 cttttcataa tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct
     6781 tgttttggtt taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc
     6841 ttgattgaaa tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt
     6901 caactctttg ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac
     6961 tagagcggca agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt
     7021 aggcttagtt aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag
     7081 aatgacaaaa tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata
     7141 ataatctgtc atggactcag gccattttgc tggttcatct gcataaacag cactgaccat
     7201 cagatagcga gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa
     7261 gtctacttgc agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc
     7321 tttgaatgct tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac
     7381 attctctttt tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg
     7441 ggaacttgga gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac
     7501 aaaaattttc atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca
     7561 aaagagaaaa aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt
     7621 gaagtatctt aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc
     7681 aggtatacca cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt
     7741 tccgatgact ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat
     7801 cgtattggtc aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg
     7861 tttcattagt ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg
     7921 aggaataaac gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat
     7981 aaacggagaa aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat
     8041 cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat
     8101 gattacagga atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg
     8161 cgtaacagct aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt
     8221 atttttcata agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg
     8281 ttcgatgatc gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag
     8341 agattctctt ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg
     8401 agtgatcggg ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc
     8461 cactggaagc gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt
     8521 gccgataatg tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc
     8581 gatatgattg attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt
     8641 cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac
     8701 atctacctgg gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga
     8761 aatacttcca gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc
     8821 tcagtaatca atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta
     8881 gataagagat agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata
     8941 aaatcagttt tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt
     9001 acatctacta atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa
     9061 gaaatgattt ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc
     9121 tttgaaacag tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc
     9181 gttgaagaag tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta
     9241 aaatcactga ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct
     9301 tctaattgta aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgc
     9361 cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca
     9421 gctaaataat cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt
     9481 acttgatcga ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac
     9541 ctacatttac tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag
     9601 gcaattgatc cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca
     9661 tctgttgcca taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca
     9721 actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat
     9781 tgccacataa agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct
     9841 cgccacctac tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct
     9901 ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga
     9961 cagactcttc ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg
    10021 ctatcgctgt actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg
    10081 tcataccttg acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa
    10141 ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta
    10201 ctttttgata ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa
    10261 gtacaagatc ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat
    10321 caatccctgg agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg
    10381 catctttata attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact
    10441 cagtaaatgg cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag
    10501 atcgaaaagc gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa
    10561 gcctactctt ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag
    10621 aatcaaacaa ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac
    10681 aaagggttgc ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt
    10741 ttactattag aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat
    10801 ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa
    10861 gggtatattt cccagttgga acgagattta agttcaccat caatggaaac attcttttct
    10921 attcttgaag tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg
    10981 caagtggttt attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa
    11041 ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt
    11101 gaaggtgcag gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta
    11161 ttagaagggg aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag
    11221 tcgatctatt atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca
    11281 aaagtattga ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa
    11341 accaatcatt tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa
    11401 agtaagtttt gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg
    11461 taaaacaacg atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta
    11521 ttttgaaggg aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt
    11581 ccaagattat gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa
    11641 gatcaagaaa atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt
    11701 ccagcttcct ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg
    11761 tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt
    11821 atcggcgctt gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca
    11881 acgattagga atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag
    11941 tgatgagatt tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat
    12001 ttatgatgag ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga
    12061 cggtgtaatg atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga
    12121 tggcgggatg cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat
    12181 cacgacacca gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt
    12241 ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa
    12301 aaaagctaaa gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat
    12361 gcgtttcaac gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg
    12421 gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt
    12481 ttattcgtga tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg
    12541 caattcacgc tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg
    12601 atcaattctg tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg
    12661 actgcttatt ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg
    12721 cctacatggg tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac
    12781 ggcagcgtga atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca
    12841 gacttcagtt ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt
    12901 ttcaatgcat tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg
    12961 aatagctttg agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt
    13021 ggtgtccaag cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc
    13081 ggaaatcggg tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac
    13141 tgggggatgg gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc
    13201 ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat
    13261 ctttatttag ggatcgtctt tttattctta tatgcaccga tcttctatct gattttttat
    13321 tccttcaatg aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa
    13381 gctgtttttg aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc
    13441 ttatctgcat tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg
    13501 aaaaaacgac gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct
    13561 gatgttatta tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt
    13621 tttcagagtg tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc
    13681 ttgccaaaat tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct
    13741 aataatttcc aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct
    13801 ggatatttta tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca
    13861 ggaaatggct ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt
    13921 ttagaaatca atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga
    13981 tactacttta ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc
    14041 gagggggtgg cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca
    14101 ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag
    14161 caaaggtact taccatctat aactggggag attatatcga tcctagtttg atcacgaaat
    14221 ttgaaaaaga atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt
    14281 tcacaaaaat ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga
    14341 tccaaaaaat gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat
    14401 taaataatat tgatccacga tttttagact tggattttga tccagataat cagtattcga
    14461 ttccatattt ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc
    14521 aaattaagca ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg
    14581 acggtgcacg agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta
    14641 agaatatggc acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca
    14701 aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag
    14761 tgacattttc tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga
    14821 tcccaccaga aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa
    14881 ataaagaagg tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa
    14941 atgcagagta tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag
    15001 acatttcgtc ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct
    15061 acaaagatct aggatcggaa tacctaggca tctataatga cctattctta gaattcaaga
    15121 tgtataggag ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc
    15181 taaaaatcgt cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc
    15241 cgcttttttt gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca
    15301 aaaccattgt aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag
    15361 aaggataaag taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa
    15421 aactaatata tgaatccagt tcaatatttg tagctatgaa aaaacataaa aatcatatta
    15481 gataaaaact aaattggtag ataaattatt ttgtcagata tctgaggcaa atattcgact
    15541 atttgtgttt caaagtaaaa attggaatac aaatatagaa tagacatatt gcagccgata
    15601 gtttattatg cttttagaca cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat
    15661 cgagtttttg atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa
    15721 atttcgaaga atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg
    15781 gaagataaaa aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa
    15841 tatgcggttg tagataatac ggagcaaata aacgaagcct ttcattctgt agttggtcat
    15901 gacggaagtc tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac
    15961 ggactcagtg tattaataaa tggagccacg ggtgttggaa aaagtttttt agccacaaaa
    16021 atttttgaat acgctatcca tgagcaaatt attgaaaaag atgcaccatt tgctatctta
    16081 aactgtgccg attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag
    16141 aagggggcct ttacgggggc tgaaaaggat accgatggtt tattggcaac ggccaacaac
    16201 ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt
    16261 ctatttattg atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct
    16321 aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa attatttatt agacaccttt
    16381 gatcggcgta ttcaaattag tgttatgttg ccaacttttg aagatcgccc cattcgagaa
    16441 cgattagagc tgattcaatt attttttcaa aatgaagcgg atgttttaca aaaagatatt
    16501 attgtttcaa gagaagcctt gtctttaatg ttatctcatc cctttagtgg gaatattggt
    16561 aagctgaaga atatcattaa aatcagttgt gcggatgtgt atagtcgaac acaagaagaa
    16621 ccattaacga ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta
    16681 gaaagcttgc cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga
    16741 atcaaaccgt ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt
    16801 aatttcaatg aaaatttttc ttcgataaag aatgaagtac agcatttggt gcatcacatt
    16861 gataaggatt cttttttatc aatgagtaga aaattgatga ttcagatgtt tagaaaggtc
    16921 tgggtatccg ttttaggaaa aacgtacgga ttgacgacga gtggacctgt tgcagaaatt
    16981 attagcagat cctatgtcat ttccccaagc tataagacag acttaaccgg gataaatcgt
    17041 acattagctg caaatctacc tcggacgact tacttatgta atcagttctt ggagcattta
    17101 ccaccgctct cagaaaagga cgagaacttt ctaagactgc ttattgtcgt gactttatct
    17161 gattatgtag atgaatccat cgagctgaag ggcctgttgc ttgctcacgg agatagtacg
    17221 gctagcagca ttcaagcagt agtgaatcaa ttatgcggca attatgtttt tgaagcgttg
    17281 gatatgccag tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa
    17341 cggcaagtga atacagatag tctagtgctg atcgtagata tgggctcttt aaatcaaatt
    17401 tacacacaga tcaagaacga tttaaaaggg gagttacttc ttctgaataa tttgacgact
    17461 tccattgcac ttgatattgg cttgaagatg acgatgaatg cgccatttaa acagattgcg
    17521 gaggatgcag cagacaagta ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt
    17581 agcaacatta tcatttcctg tatgtcgggt ttggggattt cggacaaatt gaaagatgtt
    17641 tttcgtcatc atttatcgga tgaatccatc gaagttttca cgaaggacta cagagaacta
    17701 cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca agacaaaatt ggtcatcacg
    17761 acttcggatc ttccacgttc cttttcaatt cctaatatta atgtttatga catcttagac
    17821 ggtgatggag cagccaattt ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt
    17881 aaccgcttga ttcaggagct agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg
    17941 aactttctga acccaacgat cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa
    18001 aattactatc atttcacttt aaatggccgt gtaaaactaa atctctatat gcatacggca
    18061 ttgatgatgg aacgtttatt cttggctcgg acagaaaaat ctgaagaacc gaattatgat
    18121 ttgaaaccag atgaagatga attttattat gtttctagaa gtatttttca atcaatggag
    18181 atgaaatata attttaaagt caatgcttat gagctttcgc tgctctatga attattagga
    18241 ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt taggttagac
    18301 acttttttta atgtgaatta acgtgattaa accaatgttt ttaaaagttg gcacaggaat
    18361 tgcttatata aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa ttctaatagc
    18421 gactcacggt cacttagctt caggctttct tagcagtatt caattactga cggggaaggt
    18481 tcaagagatt acggtgatta atgcttatgt ggatgagtgt gattttgaac aggagttgaa
    18541 tgtatttgta aaccaggtga acgagcagga tcagattttt gtttttaccg atttatttgg
    18601 cggaagtgtc aatcagaaga ttaccaaaac ctttttggag aaagagattg ctgtaactat
    18661 tttgtctggc tttaatctgc caattctgct cgaaatagta ttggcaacca aggaattaac
    18721 aaaggaggat attcaggaac tggtaaataa atgccaacag gaattaaaga tgaccattct
    18781 tgatgaggta catgtagtaa atgaagagga agattttttt taggaggaag aaaaatgatt
    18841 aaagcagtgc gaattgatga acgattaatt catggacaag tagcgatgac ttggacaaag
    18901 agtctaaatt taaccgggtt agtagtagca agcgatgagg cggctaccaa cgatattcaa
    18961 aagatgacat tgaagatggc tgcaccatcg aatgtcaaag tcattattaa aacagttgat
    19021 gacgcgattc atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc
    19081 cctaatgtca aagatgcggt aaaagttgcg aaagctttcc caacggaaat cgaattaatg
    19141 aacgtgggga atgctgggaa aatgacaccg ccatcggaag ataagaaaac cttatcaaag
    19201 gaagtgatgt taacgacgtc agaaattcaa gacttaaaag aattagttga actttatcca
    19261 gataccgttt ttcagcctac accggcgatg gaaaaacgtt cagctatctc tattttgagt
    19321 gatttttaga aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc gctttggccg
    19381 tttttattgg agttgctgga catgaaatgt tcgggatgtc catgctgagt cgtccgattg
    19441 ttgttgctcc gttagcagga gcgttgatgg gggatttgca gacggggttg attgtcgggg
    19501 cgtcactaga agccatcttt atgggtgtcg tcaatatcgg aatctctagt acggcggagc
    19561 cttctttggc tgctgggctg gctacagcgt tcgcaatcaa aatgggagga aacgtggatg
    19621 cgattattcc cattgtcttt ccactagcag tattaggatt acagttaatg aatatgattt
    19681 tttcttttgt atgtggaccg atggctgcta aatttttcaa atttgcaaaa tctggtgatc
    19741 aaaaaggaat catcaatctg cacttcttta tgtggtttgt ccactatgcg ctttatgctc
    19801 ttattccatt ctttgccgtt ttatttggag cagatgttgt caaaagcgtc ttggataata
    19861 ttccagaggt catcatgaac ggattaacag tcgctggaaa tctgctgccg gcagttggga
    19921 tggcgatgct gttacggatg ctttggagta aaaatattgc tgtgtggttt ttctttgggg
    19981 ccgttgttat ggcgtatctc gagttaccat tgattgcaat tgccgcagtt ggaacgatta
    20041 ttgcaattgt tatagcccaa agagatctac aactaaaaaa tatatctgtt catgcagttc
    20101 aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga ggaggatttc tttcaatgaa
    20161 attagctgat ggaatttcta aagatgagaa aaaattggtt cggaagatgt tttggcgctc
    20221 gatgactatg tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt tcgcctattc
    20281 tatgatgccc ttcattaatc atttctacaa aagtgaagaa gatcgagctg cggcgctgga
    20341 acgtcataca cagtattttt cgacgaccat tccttgcgcg tcctttgtca tggggattgc
    20401 ggcatcgatg gagaaggaaa attctgaaaa agctggtttt gacgtggaaa gtatcaacgc
    20461 gatcaaaacg agtttgatgg gaccattggc tggaattggc gattcattgt tctggggagt
    20521 ttggcgtgtc gttaccgcgg gactagcgat caacttagcc aatgccggaa atattttggc
    20581 accaattatc tttttagtta tgttcaatat acctaattac ttgatgcgat actatggcgg
    20641 cgttttaggc tattcattag gctctaaata tattgaaaag ctgtatgcga acgggttaat
    20701 tgaaattctg acaaaagctg ctagtatctt gggtctgatc atggtaggtg caatgacgat
    20761 cagtatggtc aaattcaata ccgttttgag cttttcaatg aagggcgaag aactaatgaa
    20821 tgtccaagaa atcctcgata gtatttttaa aggaattgta ccgttattgg taacattgag
    20881 ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt
    20941 cttgggaatt ttattgtcat tacttggaat cgcatagtag gaagctgcag ctactaggct
    21001 gcagtttttt tagcaaggag ggaaaagatg actattaatg aagtaattta ttcacccaaa
    21061 cacccatttt ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc
    21121 tactatcgga atattcctga tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca
    21181 ttagcaaacc ttgatgtttg cgggacgatt caaacgttga ccttttatcg agaaaattta
    21241 ctaactgagg aaaaaccagg tgtttgggtc aataaacagc tctctcaaac caattcgtta
    21301 tccttaaagg ttgaagtaaa cgggattgtc taccatttgt cagaatcgga tcatcgtatt
    21361 gaagtcgatg tgattcaaga ctgtttgcct agatatattc atcattattc gacgtttaag
    21421 gtcattgtag tatcctttgc tcccatactg gctaataagc gtttaagtat gcttgttcaa
    21481 caggtttaca ttgtgaatga aacacacgaa agccttcaag tttcagtgaa agatgtgcct
    21541 tcgtatcaag agaagtattc ggatcagcaa aatgtgttga tttctcaaaa aggaaataag
    21601 gacacgatcg cacaacagga agtggcaagc ttcgccattg cttttgtgga cccaaatgct
    21661 tttgaagaag taatgacttt tcaggaatct gatatcgaaa aatggctgaa ggaaacattg
    21721 aactattttg ctcagctatt tgggcagctg tcaatgccaa acgatagagt tgtccatctc
    21781 tatcgtcgtg cactttatca aagcttttct agtttcggta tgaatcgaca gggacaggtc
    21841 gtcggcagca attggggcag ctatcctgct actaatcgaa tttggaataa ggatatgtat
    21901 tacgcatcat tgccattcac catgtttgaa ccggagttat gtcaaaaaac ggttctatgg
    21961 tttgatcaat atggcgttaa gtttcctggt tcaaaattta caggaggtat caatcattct
    22021 ctgagtaatt cgttatccag tgcgttactg gctagtcttt attttgaaca tactggggat
    22081 acaaaattct ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat tattgaggac
    22141 atattagctc aacgtcggtc aggggaacca atgctttttc catctgtttg gttatcagac
    22201 gcctttgcac ttggaaagta tcataccggc agcaatcttt gtctttggaa ggcctgtatt
    22261 ggacttgcag aaatttttga agccctagaa caactgagtc ttagcaagcg atacaaaaac
    22321 attgcctgta aactaaagga atcaatttgt aaacaaatga ctatcgatgg tccttttggt
    22381 gagcaatttt tggagggaat cggtgatgag gagaaaacaa cctattcagt gaagcactat
    22441 caaaaaccga ttcttgagca agggttaatc tttttaagtg atgtgattgt ggatggaaaa
    22501 attaatttat tgatgcatga tggagaagaa tcagacacta cgttgatgcc tttttatcaa
    22561 tttttagata ataaagatca tttatatcag aacaccatgg ccttttctgc tagttctttc
    22621 aatccaacct atagcgagga aatcaaaggc atcacttggg gattagaatc cggcgccact
    22681 tttcctggct ttatcactgt tttaatgagc gagctgcaca accctcaagc tttcgtaacc
    22741 cgattagaag aactaatttg tttggcagat ttagacggtt cgtggtggtg gtggccgtat
    22801 cgcttagagg ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa atgcggctgg
    22861 gcatcgggtc tttttgtcag cctaatgatc agtcaatatt tcggattgaa attcaaaaaa
    22921 ggagtattgc aaattgatcc tgttgataat ttaacgtttt cttggaagaa tttgaagttc
    22981 ggtcaagcct tcatttcgat tgaatgtacg caatccgagt tgagtatttc aaatttgtca
    23041 gaaaagccat tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg
    23101 attcacattc aaaggagaaa aagatgagta tactaaaaaa tattacccaa tttaaaaaaa
    23161 tccagagtcg agcaatcact cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt
    23221 tgccaagcga gctaggacca agccgcaaag gtcgtggcgc aattccgctg acttctggtg
    23281 aagaaactgt gattgcggat attactggaa cgggaataat tcgtcatatg tggatgacga
    23341 ttcgtgatcg aacagaattt ggcagttttg ttttacgtga tttaatctta agaatttatt
    23401 gggatgggtc ggatagccct gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt
    23461 ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt taatcctaca gggggcttta
    23521 attcctattt cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag aatttacatc
    23581 cgaaggatat gtcctcattt ttttatacga taaattacgc tttggtggac gaattggaag
    23641 aaaatagcct atattttcac gcaaattggc atcgggaacg cattaccaaa ttacaggagg
    23701 attataagtt aattgatcga ttaaagggtc aaggctatta cgtgggtact taccttgcgc
    23761 ttacctgcct agaacgttat tggtggggcg aaggcgagtt taaattctac attgatgggg
    23821 atagtgagta tccgaccgta acctcaacag gttcagaaga ttattttggc ggggcgtggg
    23881 cctttcatga gtacgatgag ttcggacgtc cacatgcgaa aaactattca acagcctttt
    23941 tagggtatcc ttaccaatca aatagagatg ccacaagaga tcgttttgaa acgggaaaat
    24001 tgaatgccgt tcatgccttt ggtgacgacg caatgccacg acatggtctt tatcgttggc
    24061 atcttcaaga ccccatctgt tttaccgaag atttatcggt cactttacaa caaatcggca
    24121 acgatgatgt tcgcttattt gagcggcaag acgatgttgc tagtgtcgct tactggtatt
    24181 caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc ttgccgcgtt
    24241 aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg gggcaattga
    24301 agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga ttttggaaag
    24361 agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg atttttttaa
    24421 accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa ttgatattca
    24481 atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt ggcaaaattt
    24541 caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga caacagatgt
    24601 caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag caggtgttgc
    24661 atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga ctttcgtgga
    24721 agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa aggacaattt
    24781 ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag gaccagcgat
    24841 tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat
    24901 tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag
    24961 aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa
    25021 atttgttgag cttataaatg gatatgttga aataccacca attgatagct acattattac
    25081 gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt
    25141 aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa
    25201 ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg
    25261 ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc
    25321 ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa
    25381 atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta
    25441 tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt
    25501 gatagatttt ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat
    25561 aaaaagatag caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt
    25621 tctattgcta aaaaagtaga aattctatat ttacctttaa ctctcgcttc tcttgcaaat
    25681 gtagttagca
//
LOCUS       DANKHK010000028        25595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000028 DANKHK010000000
VERSION     DANKHK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..2068)
                     /locus_tag="PW109_001117"
     CDS             complement(<1..2068)
                     /locus_tag="PW109_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2510979.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTS"
     gene            complement(2081..2356)
                     /locus_tag="PW109_001118"
     CDS             complement(2081..2356)
                     /locus_tag="PW109_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510980.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2718..3173)
                     /locus_tag="PW109_001119"
     CDS             complement(2718..3173)
                     /locus_tag="PW109_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510981.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3327..4535)
                     /locus_tag="PW109_001120"
     CDS             complement(3327..4535)
                     /locus_tag="PW109_001120"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2510982.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4647..4979)
                     /locus_tag="PW109_001121"
     CDS             complement(4647..4979)
                     /locus_tag="PW109_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2510983.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5127..6002
                     /locus_tag="PW109_001122"
     CDS             5127..6002
                     /locus_tag="PW109_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2510984.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6084..6665)
                     /locus_tag="PW109_001123"
     CDS             complement(6084..6665)
                     /locus_tag="PW109_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2510985.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6671..7930)
                     /locus_tag="PW109_001124"
     CDS             complement(6671..7930)
                     /locus_tag="PW109_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2510986.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7932..8978)
                     /locus_tag="PW109_001125"
     CDS             complement(7932..8978)
                     /locus_tag="PW109_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2510987.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9213..9488)
                     /locus_tag="PW109_001126"
     CDS             complement(9213..9488)
                     /locus_tag="PW109_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2510988.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9731..11041)
                     /gene="der"
                     /locus_tag="PW109_001127"
     CDS             complement(9731..11041)
                     /gene="der"
                     /locus_tag="PW109_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2510989.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11223..12452)
                     /gene="rpsA"
                     /locus_tag="PW109_001128"
     CDS             complement(11223..12452)
                     /gene="rpsA"
                     /locus_tag="PW109_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2510990.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12587..13267)
                     /locus_tag="PW109_001129"
     CDS             complement(12587..13267)
                     /locus_tag="PW109_001129"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2510991.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13316..13951)
                     /locus_tag="PW109_001130"
     CDS             complement(13316..13951)
                     /locus_tag="PW109_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2510992.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(14017..15420)
                     /locus_tag="PW109_001131"
     CDS             complement(14017..15420)
                     /locus_tag="PW109_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2510993.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(15407..16438)
                     /locus_tag="PW109_001132"
     CDS             complement(15407..16438)
                     /locus_tag="PW109_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2510994.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16469..16690
                     /locus_tag="PW109_001133"
     CDS             16469..16690
                     /locus_tag="PW109_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2510995.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16855..17592)
                     /locus_tag="PW109_001134"
     CDS             complement(16855..17592)
                     /locus_tag="PW109_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2510996.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17745..19085)
                     /locus_tag="PW109_001135"
     CDS             complement(17745..19085)
                     /locus_tag="PW109_001135"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2510997.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19322..20845)
                     /locus_tag="PW109_001136"
     CDS             complement(19322..20845)
                     /locus_tag="PW109_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2510998.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(21223..21834)
                     /locus_tag="PW109_001137"
     CDS             complement(21223..21834)
                     /locus_tag="PW109_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2510999.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(21957..22082)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(22164..22880)
                     /locus_tag="PW109_001138"
     CDS             complement(22164..22880)
                     /locus_tag="PW109_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2511000.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(22886..23455)
                     /gene="scpB"
                     /locus_tag="PW109_001139"
     CDS             complement(22886..23455)
                     /gene="scpB"
                     /locus_tag="PW109_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2511001.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(23439..24236)
                     /locus_tag="PW109_001140"
     CDS             complement(23439..24236)
                     /locus_tag="PW109_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2511002.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(24206..24577)
                     /locus_tag="PW109_001141"
     CDS             complement(24206..24577)
                     /locus_tag="PW109_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2511003.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(24596..25483)
                     /gene="xerD"
                     /locus_tag="PW109_001142"
     CDS             complement(24596..25483)
                     /gene="xerD"
                     /locus_tag="PW109_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2511004.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         7289 a         5481 c         4310 g         8515 t
ORIGIN      
        1 gactcgtttt ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt
       61 tgttcatcgt ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg
      121 tcgtgatttt ttcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc
      181 ttagcatttt tttcgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc
      241 ccgagttcat ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt
      301 catcataaat atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat
      361 atgtctctct gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatatc
      421 ttctttgcca aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga
      481 accaaaagcc gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa
      541 aacgggagta aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca
      601 atccatcccg ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat
      661 catcggttaa atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg
      721 cttcagtggc taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa
      781 cttcaacgac ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg
      841 tttccgtcag cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg
      901 ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca
      961 aaataaaaat cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga
     1021 tctcgttcaa ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga
     1081 tagaaatgaa tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa
     1141 gaccgtttgg atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc
     1201 ctaaaaatat aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg
     1261 acaagctcga caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa
     1321 tacctgctgc cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg
     1381 ctagaaaagg aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt
     1441 tttcgatcaa atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc
     1501 ccccaatact agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga
     1561 tcagcgcacc aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt
     1621 ctccttccaa aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga
     1681 ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga
     1741 cacttggcaa caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc
     1801 caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt
     1861 ctccttctct aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat
     1921 cgattgattg tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg
     1981 gagctggaat cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa
     2041 atacaataac taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa
     2101 gcatttttaa aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg
     2161 tttggttccc ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt
     2221 tcagacgttc caactgacga tacatcaata tatttctttt acgcaactta caaagtattt
     2281 gacggcggtc atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt
     2341 caaaactctc tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt
     2401 tctttttgga tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat
     2461 aaatttttca acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa
     2521 agataaaagt ttctgacaaa aggatgttta ctttcccatt cctgcctttc ttgttctttt
     2581 atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa
     2641 acggtgtgga agagacagcc tatcagtagt aataagacac atctctttca cagcctcatt
     2701 tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct
     2761 ggattgatca cgatatacaa gttacctgtt ttttcgctga tttttgttga ttgatattga
     2821 ttgtctaatc cttctgttcc tttatctaga tatgggaagt acacaagatc aggtgtgcca
     2881 tttcggtaaa ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg
     2941 cccatttcca gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag
     3001 atttcgattt taaatccagc acacggatgg atttcaacaa attcactgcc attgattcgt
     3061 ccaaaacttg ttgtgatttg tttgatccaa agatcgccga tatgacggcg gttgatcgtc
     3121 caggtttctc catttctaaa ataaaatttt agcgctgtca tttctttact cattaaaaag
     3181 actcctttat gatgtaattt attcttgtac tacctcagtt tagcacaaat ttatgtaagt
     3241 ggtcacatct tatgtttttt cttctaatga cgtatctgcc cactttaagt agccaacaaa
     3301 gctgagcaga tacagtctag tgttttttaa atcaattttt ctttcgataa aaaatccaaa
     3361 acctgttttt tatcattttt tattctttgt tctacgacgg ctttttcagc taattgcaaa
     3421 gcttcactaa cccacttacc aggttttttt ccagtttctt ctagcaagat tttaccatct
     3481 actgctaatt cgcttaatga cttgataggt aaacgatcat aggattctcc tacggcatcc
     3541 atctgaggtg tttcgtcaaa atagatcatc aattcttcta cttgcagtgc atgttttttg
     3601 cctaagaagt acaattcgaa tggttccaaa gctccagttt ctcgttgtcg tagtccttgg
     3661 acaagtgctt gaacgtcttt aatcatttgg ttagaacact tccacgattt caaaaaggaa
     3721 cgaatctcat tttcttttag atccagttgg ctgatcaata atgcccatgc ttggctctct
     3781 tctttcaatg ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc
     3841 aaaccgggac agcaaatgta gcactctgtt tcaataaatg catggattcc cgcggcccgt
     3901 tcatttccca agagcatctt gatgaattcc acatttatac gttctacaga tattttacct
     3961 aatagctcgt ggttttcttt gatggcgtag aaggtttctt cctctaattt gaaccctagt
     4021 tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga
     4081 ggattgccta cagctcgcaa gatctgttct tctagatctt gcagaccatc gaacaaatcg
     4141 atgatttctc catcttcttt caaagcaaac gcattgatcg taaaatctct tcttttcaaa
     4201 tcttctgcca acgaacgaac gaattcgaca tgatctggtc tacggtagtc ctgataagtt
     4261 gattccgtcc gaaatgtggt gatctcatat tgttcatcat tttctaatac caacacagtt
     4321 ccatgttcga tcccaatatc tatcgttttt ggaaaaagct gtttgatttc ttctggaaat
     4381 gcgcttgttg cgatatccac atcatggatt ggctggttca gcaagatatc acgaacactt
     4441 ccacctacaa aataagcttc atatcctgca ttctctaatt ttttcagaac tggaattgcc
     4501 ttttggtatt ccagaggtaa ttgttttaat ttcatttttc caacctttcc tgcgtgtttc
     4561 ttgtcctgag ataaagactc agttgcatgg tgactgtttg acaagtccgt ttcagagtct
     4621 tcaaggccag gaccacttca tcttgtttac tcgtccttac gctcaaagcg attatggtct
     4681 ctttgattga atttttccat gtttttttca aaaacttcag ttaagtcgat attcaatgaa
     4741 ttagccatga tcatcgtaac gaataaaaca tcaccaagtt cttctgctac cgagttaact
     4801 ggttcagaag cctttttgct tttttctcca taagtgtggt tgacttctct tgccaattca
     4861 cctacttctt cagttaatcg agccatctga gctaatggag agaaataccc caccttgaat
     4921 tgctggatat attcatccac ttcctgctgc atactgtata aagaacgctt tttatccatc
     4981 tttttccctc caaaactatc ttaccaaaac tttaacgcaa atcctagata agagcaattg
     5041 agaaaatcga atttccatgc tacatttagg aaggaatttc taaatggaaa ttctgtgaaa
     5101 atacataatt gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt
     5161 ttatcattat aggaacaagt atttatgcat ttggtcttgt ttacttgaac atcgctaatc
     5221 atttagcaga agggggagtc tcagggatca ccttgatctt aagagcttta tttggtattg
     5281 atccagctta tacgacttta ttaatcaaca tcccattgat tttactagga ggaaagatcc
     5341 tcggtaaacg ttccttggcc tatactgttc taggtacact ttctttgtca ttctttttat
     5401 ggatctggca aagggttcca ctagaaatca atttacagca tgatctactg atcgtttctc
     5461 tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa
     5521 caggcggcag tgatattatc gctcggattt tagaacaaaa ttacggcatc actatggggc
     5581 gttccctatt ggcatttgat atcatcgtct tacttgcttc attaacctat atcgacttga
     5641 aacgtatgat gtatacgttg attgtttcgt atgttttcag tcgtgtgatt gattcgatct
     5701 tagatggcgg atattctgca aaaggtctac ttgtcgtctc caacaaaagc gaagagattg
     5761 caccactact gatgaccggt ctgcaaagag gtgtgacttt cctttctgga gaaggcgcat
     5821 tctctggtaa ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa ttaactgaag
     5881 ctaagaggat cgtccacgag attgacgaaa aagctttcct ttcaatcatc aatgtgcatg
     5941 aagtggaagg gcaaggcttt acttatttga agccacagac aagattactg aaaaaactat
     6001 aagaaaaaag acaaagatag gctatgagga aagattccac atccagctga cctttgtctt
     6061 ttttagtact atactatttt taactaattg ttatgttcac ttacccggtt acttttcttt
     6121 tgtgtctgca aaaggatctt tcggttaact tcatcagata atttcaaaaa agcatcccta
     6181 tctccatctt ctagtgcttg atcgatttga gcataaagca tttttagttt tgcctcttct
     6241 tctttttctt gaaaatatcg ttcaattaat tcagtatctt cttcactgac tgaagaatcc
     6301 caagaagaaa atggattgtc ttctaagata gatagatatt ctcttgtctg ccaagcattt
     6361 tcgaatatac attccacata taaaggcttt ttccaattca aacggatttc atgaaaaatc
     6421 tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc cccttcaaca
     6481 gatagatcac tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca
     6541 ttgcttaaaa tcgcttcatg attggcaaga taattcaaga tccaaaaaac ttctcttcgg
     6601 ctaaaagata catgattgac catccacgtt aaaaaattct tcttttcccg tacatcaatt
     6661 agcatttttc tcacctctct gttaggtcat ctaataatga cagtgcctcc atatctcctg
     6721 gttcaagtgt taaataataa gtcaataatt cttgcgctct tttcagctgc ccttcttctc
     6781 ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg tgatagttca tgataagcct
     6841 gttcatagtg tacagctgca acggtaaagt cttctaattc attataagca tgggctaggt
     6901 tccattctgc ataaggatta gcagtttctt ccatctgatt gatcacttgt atcacatctt
     6961 catatctttc atcatccaag taaagattac ttaaagtaag taatgtttca tcttgtttgt
     7021 ctcctacttc taaagctttc agtaaaagtt cttctgctcg ctgtctatca tgcagacgga
     7081 aagcattttc cgaggccagg tgataaagtt cgacttggta aggattttct ttgattcctt
     7141 cttctaacac tgtttgtgct tcttcaatca tttcttcttc ttgaagagct tgtcctagat
     7201 atagatataa cgattgataa tgcggattaa cttctcgaag ttcttccaga tagttgattg
     7261 cctgctgatt ttctttcaat tgaagattcg taaaagctag ctggaacagt cgatcatctg
     7321 tcctttcttc tttcagtgct tcttctaaag gtacgatagc tgcttcgaat tctccttgca
     7381 tacttaagga ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca tttatcccgc
     7441 ttgccaacaa tgtttcataa accactgctg cctctttgaa acgatcaact gaaaaataca
     7501 attcagctag cgcaaactgg atcaatggtt catcaggaag caacccagct gcttctttca
     7561 attttgcttc actcacttca gggattccga taacttggta caaatcagct atcgccaata
     7621 agctttgcgg atagtaatca ctttgcttat ccactttttc caggtattca aaagctaagt
     7681 cgatctcatt atcttcgatt gcaatttctg ctaacgggat gttcagtccg tcattttctg
     7741 ggtattggtt gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca
     7801 gcaattcttc gcctagttca gctaatatat ctgctgggtc ttcttttaat gctttttcta
     7861 aaaagatttg tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt ttttcgctat
     7921 ttgtattcat ttcaattccc tcttatctca ctgacgattt gttgaaatac cgtcattgtt
     7981 ctcaataatt ctttttcgtt tggatgttgg tatccgtcta ttatacctat tttttctaaa
     8041 acaagtattt ttttgctttt ttctgccaaa aaaagaactt tttccaaata ctccatcttt
     8101 gaaatctgtt ccagccagtg gattggatag ccaagcaagt ccagccacac aaaaaagttt
     8161 tttagttgga aatcaaaagg atatgcttgg agattccaca cgaaatgaaa catcattcct
     8221 aaaaattttt tcatactagc agaaagtaga tggccatttt cggtcagata aaaagcttgt
     8281 tcaaacagtt taccataatt tgacaggttt tctgcctcta attgataaaa atagatcatt
     8341 tcttcgatca tcccggcaaa cggccgagtc gttaattgct ttttatttgg atagttttta
     8401 tacagatttt gcaaaaaacg atgatcacaa atcattccgc aacagataaa agtcaaaaag
     8461 tcaatctgct ttccgtccac ctgccgctcg ctgattgttt gatcataaac gatgcgttct
     8521 ggcagattga tggtttgcag cactacacga tttggtagct gcaaaatcgt ccgctgcggt
     8581 gttaatgctc ggcttagaga acggatcgat acagggatca tccatagtga tgaatcaagg
     8641 gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc cttcatttcc aaatccgatg
     8701 ataaacatgg attgattttc gggatagcgt ttgacgaaat caagcaggtt ctttaattct
     8761 gttaaatgat tgcattgagt gttggcacaa atataccagt cattgtttgc ttgatttgca
     8821 aataacgggt tgatcttatc tgcaaacaaa tcataatagc gctgattcgt taaaaataag
     8881 attggctgat caatggaaag ttctcttagc tgactgacta gcgtttctcc gtaaaacact
     8941 ttcgtcaacg catcttctac ttgataatca agttccatac gcatccctcc tcttcttatt
     9001 tttattttac cattagtaag caaattctag aaaagataag agcaaagctc acctcataac
     9061 ttgcaaataa ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc
     9121 ggaatctctg tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta
     9181 gccctagagc cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta
     9241 cctggtttga atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg
     9301 ttacgacctt tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga
     9361 actttttcgc cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca
     9421 gttgcatctt ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg
     9481 tttgccatgg atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa
     9541 aactttctga gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta
     9601 tcacgatatc atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt
     9661 tagtcataga aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat
     9721 ggtaaaatgc ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa
     9781 atgcttttct gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt
     9841 cattgacaaa aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct
     9901 tcaggcgttt tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat
     9961 cattcaacac agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt
    10021 caggcagacg ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat
    10081 caagataccg gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt
    10141 tctttacaag atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt
    10201 aacccgctac tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa
    10261 cgatatcaga acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt
    10321 cgtatacttt tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac
    10381 tttcagaaac gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa
    10441 ttacacgttc ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac
    10501 caatcaaact aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact
    10561 tcactgcttc atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag
    10621 gatcgcctag ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta
    10681 ctttattgac tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct
    10741 catctgcgtc tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt
    10801 caatcgcgat ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa
    10861 ttccgcctgt gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac
    10921 gatcacgagt tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt
    10981 taaaaatcgt agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca
    11041 tattatttcc tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt
    11101 tcaagcatag attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct
    11161 tttcaaaaga aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc
    11221 aattattttt cagaatcatc ggttgctttt tcttctactt cttctttcaa cgcatcacct
    11281 aaaatgtctc ccatagtaaa tccagtattt tcttctggta attcataaga ctcaacattt
    11341 tttggttctt cttctgaagc tggtttttct tctaaagctt tgatactcaa tgcaatacga
    11401 tgttcttctg gatggatctc taatactttc actttcactt cgtcaccttc atgaagcact
    11461 tcatgtggtg tcgcaatatg tttatgtgaa atttgagaga tatggactag cccttctact
    11521 ccagggaaca cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat
    11581 actgtgccaa ctggagcttt tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg
    11641 attgacaagg aaacacgttc ttgttcagga ttgatagaca acactttgac atttacatca
    11701 tcattgacag ccaatacatc acttggtttt gctacatgac tatgtgaaat ctctgagaca
    11761 tggactagac cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca
    11821 acacgtccac tgagaacatc tccctcatgg atagttgaaa gaatttcttt tttcttttct
    11881 tctctttcgt tttccactac cgctttatgc gataagatca aacgattttc tgatggttcg
    11941 atttcgatga ttttaaagct caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa
    12001 tgatcttcca ccattgatgc agggacaaaa ccacgtacac cgacgtcaac gactaaaccg
    12061 cctttgacaa catttgtaac aggtgcttcg atgaccttgc cctcttggaa atctctttcg
    12121 atatcttccc aaactttttt agcatctaaa cggcgttttg aaagtagata gctgccgttt
    12181 tctttatctt tgccgatcgt actgataacg actaaatcta atacatcacc gacttttact
    12241 acttcgctga tatcttctac cggcaaagtt gataattctt ttgccggaac gactccttca
    12301 actccagccc cttcgattcc gacaatcact tgtttgtctt caatgactaa aacttcgccc
    12361 ttaacaacat cgccaacttt tacttcttgt acgcttttca ttgcgtcttc cattgactgg
    12421 ttgttctctt gattttgatc aaattctgtc ataacacttg tcctcctaac caacattctg
    12481 ccgtaagtac ggtactgttt ttagtttaat tgattattca aaaaaaataa agcgatttcg
    12541 ttcgttttcc ttgaaaaaac aattgttttt catttttttt cttaatttaa aaaagaagat
    12601 aacctttttt caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag
    12661 tcgtatcgat acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat
    12721 cataatggtc tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt
    12781 tttcttggtt ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga
    12841 ttttcacttc tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc
    12901 caccgcgttc ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg
    12961 cagagacttc agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt
    13021 ttgatgctat gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt
    13081 tgatcagcga aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat
    13141 aagttactgc gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta
    13201 atatcttggc aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt
    13261 gtgtcatgat ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt
    13321 taattcttaa ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt
    13381 caaacaattg atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg
    13441 cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat
    13501 tgctgcttga atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg
    13561 ctgcaatact gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga
    13621 ttgtactaga ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac
    13681 tttctactac tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc
    13741 catttctgat ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca
    13801 aaaatagcga aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg
    13861 aaacttcatc atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt
    13921 ctgtagattg atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt
    13981 attataccat aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa
    14041 taaatttagt aaaatcgttt cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt
    14101 tggcagaagt tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag
    14161 tttttcgcca aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg
    14221 aagcatctgc tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca
    14281 attttctggt ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa
    14341 gggagcttgc tctgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga
    14401 aagttgatta aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc
    14461 actttcttcc tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc
    14521 tggcaaatca taatggacca cataacggat atcagattta tcgatcccca taccaaatgc
    14581 attcgtagca atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct
    14641 ttgagctgca tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg
    14701 atacaatcgt tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct
    14761 ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat
    14821 attttgccga ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg
    14881 tatatctctt gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc
    14941 taaaagcttt cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg
    15001 agacacgcaa tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc
    15061 taaaagagaa ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag
    15121 atgacggagg acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg
    15181 tttctcccct cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga
    15241 cacgatgatg accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc
    15301 cgttcctgta ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc
    15361 ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat
    15421 tctgtatcgc ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta
    15481 attgtccatc tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt
    15541 ccaaacgttc gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca
    15601 aggcccactc aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga
    15661 tctggtcaat tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga
    15721 aattttgctg taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga
    15781 tttgtgctaa caaaaggtgg atacatctgc tttgttgcag ataaaccgct gactcatcat
    15841 tgttttttgc cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag
    15901 ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg
    15961 cagtatcaat caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga
    16021 aagtataaaa aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag
    16081 aaattacttg aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc
    16141 ggtcaaaacg caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag
    16201 gcgttagctt tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca
    16261 aaatttgata aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt
    16321 cgtgggtaaa tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa
    16381 gagaactaat ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat
    16441 tattctcctt aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg
    16501 ctgtattgca tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga
    16561 aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga
    16621 agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa
    16681 taaaatataa atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat
    16741 aagccgggat ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg
    16801 ctatctatag ttaattgact agttttttca cggaccctat aggacccatt tatattattt
    16861 caactttttc agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga
    16921 aatttctgcc tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc
    16981 gccattgatt gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc
    17041 ttcctgattc aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc
    17101 tgtcaatgtt ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt
    17161 gtctgaatga ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc
    17221 gatcacataa tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat
    17281 cactgctaga tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc
    17341 ttgcgcccaa atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa
    17401 tggagacttt ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt
    17461 tccttctttt accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata
    17521 gtaataaccg gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct
    17581 aaatattttc actttctccc accttttcta tctgagtgac ccctttttct taatcaagta
    17641 tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag
    17701 acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa
    17761 cgatacacag cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt
    17821 tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac
    17881 tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca
    17941 gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat
    18001 aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt
    18061 aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt
    18121 tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt
    18181 tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct
    18241 aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata
    18301 gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct
    18361 tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc
    18421 ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca
    18481 gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa
    18541 attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta
    18601 tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt
    18661 gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa
    18721 ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg
    18781 aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag
    18841 ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct
    18901 ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc
    18961 acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt
    19021 cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt
    19081 ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac
    19141 ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa
    19201 caaatgatga aagaaaaaac actcactgaa tggagctatt gacttatcca cgtgcttatc
    19261 gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg
    19321 tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta
    19381 atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca
    19441 ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag
    19501 tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg
    19561 ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa
    19621 atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta
    19681 agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta
    19741 ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag
    19801 actctttcat gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa
    19861 tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta
    19921 ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg
    19981 ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa
    20041 catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct
    20101 tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg
    20161 atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc
    20221 gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa
    20281 aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag
    20341 ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa
    20401 tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg
    20461 gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta
    20521 gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga
    20581 ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat
    20641 tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg
    20701 atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag
    20761 taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa
    20821 attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa
    20881 ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt
    20941 ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcaggttt
    21001 ttaaagcttt cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt
    21061 cttttaaaag gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc
    21121 aaaaaaatac tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt
    21181 tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg
    21241 acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac
    21301 taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag
    21361 tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa
    21421 gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt
    21481 tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa
    21541 ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa
    21601 attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc
    21661 aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc
    21721 gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc
    21781 gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa
    21841 tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac
    21901 tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct
    21961 atcttcttta tccagactat actgtcggta tcggaatttc accgattcag ctattttttt
    22021 gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga
    22081 accttatata atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc
    22141 taacacttat aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt
    22201 ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt
    22261 tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga
    22321 ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat
    22381 aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa
    22441 ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact
    22501 tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc
    22561 cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa
    22621 agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt
    22681 ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca
    22741 gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca
    22801 cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt
    22861 actttctgca atctctccat tacaattatt ctcctttatt cattccatca aagaatagat
    22921 ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta
    22981 atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac
    23041 ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg
    23101 ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat
    23161 aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg
    23221 catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg
    23281 tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca
    23341 ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga
    23401 tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg
    23461 gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg
    23521 atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta
    23581 gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct
    23641 tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact
    23701 ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt
    23761 ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt
    23821 tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat
    23881 ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat
    23941 aattcttcat cgtctgtgac gaattcttgg acaggaagca atgtcttgct tttgattgcc
    24001 atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt
    24061 gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg
    24121 atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat
    24181 ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc
    24241 caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta
    24301 attcatctaa catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt
    24361 catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg
    24421 ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa
    24481 tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag
    24541 caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag
    24601 cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa
    24661 tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc
    24721 cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt
    24781 aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac
    24841 ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac
    24901 gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc
    24961 gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca
    25021 atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg
    25081 cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt
    25141 ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca
    25201 tcggatcgtg atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg
    25261 agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac
    25321 ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat
    25381 gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt
    25441 caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc
    25501 ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag
    25561 aaaaagccgt ctagacactc agacggactg gtttc
//
LOCUS       DANKHK010000029        25073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000029 DANKHK010000000
VERSION     DANKHK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            12..84
                     /locus_tag="PW109_001143"
     tRNA            12..84
                     /locus_tag="PW109_001143"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PW109_001144"
     tRNA            88..160
                     /locus_tag="PW109_001144"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PW109_001145"
     tRNA            168..249
                     /locus_tag="PW109_001145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PW109_001146"
     tRNA            267..339
                     /locus_tag="PW109_001146"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PW109_001147"
     tRNA            351..422
                     /locus_tag="PW109_001147"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PW109_001148"
     tRNA            439..524
                     /locus_tag="PW109_001148"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PW109_001149"
     tRNA            535..608
                     /locus_tag="PW109_001149"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PW109_001150"
     tRNA            618..691
                     /locus_tag="PW109_001150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PW109_001151"
     tRNA            711..783
                     /locus_tag="PW109_001151"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PW109_001152"
     tRNA            811..884
                     /locus_tag="PW109_001152"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PW109_001153"
     tRNA            898..971
                     /locus_tag="PW109_001153"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PW109_001154"
     tRNA            995..1084
                     /locus_tag="PW109_001154"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PW109_001155"
     tRNA            1097..1170
                     /locus_tag="PW109_001155"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PW109_001156"
     tRNA            1189..1264
                     /locus_tag="PW109_001156"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PW109_001157"
     tRNA            1266..1336
                     /locus_tag="PW109_001157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PW109_001158"
     tRNA            1350..1423
                     /locus_tag="PW109_001158"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PW109_001159"
     tRNA            1448..1535
                     /locus_tag="PW109_001159"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PW109_001160"
     tRNA            1557..1628
                     /locus_tag="PW109_001160"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PW109_001161"
     tRNA            1651..1740
                     /locus_tag="PW109_001161"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PW109_001162"
     tRNA            1753..1826
                     /locus_tag="PW109_001162"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PW109_001163"
     tRNA            1845..1917
                     /locus_tag="PW109_001163"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PW109_001164"
     tRNA            1921..1993
                     /locus_tag="PW109_001164"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PW109_001165"
     tRNA            1998..2068
                     /locus_tag="PW109_001165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PW109_001166"
     tRNA            2081..2154
                     /locus_tag="PW109_001166"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PW109_001167"
     tRNA            2166..2237
                     /locus_tag="PW109_001167"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PW109_001168"
     CDS             2503..3696
                     /locus_tag="PW109_001168"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2511005.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PW109_001169"
     CDS             3826..5298
                     /locus_tag="PW109_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2511006.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PW109_001170"
     CDS             5381..6169
                     /locus_tag="PW109_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2511007.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PW109_001171"
     CDS             complement(6236..6904)
                     /locus_tag="PW109_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2511008.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PW109_001172"
     CDS             7019..8731
                     /locus_tag="PW109_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511009.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="PW109_001173"
     CDS             8734..10503
                     /locus_tag="PW109_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511010.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="PW109_001174"
     CDS             complement(10708..11274)
                     /locus_tag="PW109_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2511011.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="PW109_001175"
     CDS             complement(11274..12230)
                     /locus_tag="PW109_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2511012.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="PW109_001176"
     CDS             complement(12230..12886)
                     /locus_tag="PW109_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2511013.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="PW109_001177"
     CDS             13256..15274
                     /locus_tag="PW109_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2511014.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="PW109_001178"
     CDS             15701..18115
                     /locus_tag="PW109_001178"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2511015.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="PW109_001179"
     CDS             18463..20016
                     /locus_tag="PW109_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2511016.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="PW109_001180"
     CDS             complement(20397..22181)
                     /locus_tag="PW109_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511017.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="PW109_001181"
     CDS             complement(22196..23917)
                     /locus_tag="PW109_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511018.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="PW109_001182"
     CDS             24017..24778
                     /locus_tag="PW109_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2511019.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLHDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7737 a         4617 c         5349 g         7370 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttc atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcgaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgt
//
LOCUS       DANKHK010000030        24344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000030 DANKHK010000000
VERSION     DANKHK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..838)
                     /locus_tag="PW109_001183"
     CDS             complement(<1..838)
                     /locus_tag="PW109_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2511020.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1062..3002)
                     /gene="thrS"
                     /locus_tag="PW109_001184"
     CDS             complement(1062..3002)
                     /gene="thrS"
                     /locus_tag="PW109_001184"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2511021.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3339..3518)
                     /locus_tag="PW109_001185"
     CDS             complement(3339..3518)
                     /locus_tag="PW109_001185"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2511022.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3565..5388)
                     /gene="cadA"
                     /locus_tag="PW109_001186"
     CDS             complement(3565..5388)
                     /gene="cadA"
                     /locus_tag="PW109_001186"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2511023.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5666..7945
                     /locus_tag="PW109_001187"
     CDS             5666..7945
                     /locus_tag="PW109_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2511024.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8038..8685)
                     /locus_tag="PW109_001188"
     CDS             complement(8038..8685)
                     /locus_tag="PW109_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511025.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8864..9583)
                     /locus_tag="PW109_001189"
     CDS             complement(8864..9583)
                     /locus_tag="PW109_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2511026.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9714..10865)
                     /locus_tag="PW109_001190"
     CDS             complement(9714..10865)
                     /locus_tag="PW109_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2511027.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11082..11822)
                     /locus_tag="PW109_001191"
     CDS             complement(11082..11822)
                     /locus_tag="PW109_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2511028.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11826..12149)
                     /gene="rsfS"
                     /locus_tag="PW109_001192"
     CDS             complement(11826..12149)
                     /gene="rsfS"
                     /locus_tag="PW109_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2511029.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12206..12799)
                     /gene="yqeK"
                     /locus_tag="PW109_001193"
     CDS             complement(12206..12799)
                     /gene="yqeK"
                     /locus_tag="PW109_001193"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2511030.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12783..13433)
                     /locus_tag="PW109_001194"
     CDS             complement(12783..13433)
                     /locus_tag="PW109_001194"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2511031.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13443..13754)
                     /gene="yhbY"
                     /locus_tag="PW109_001195"
     CDS             complement(13443..13754)
                     /gene="yhbY"
                     /locus_tag="PW109_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2511032.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13770..14879)
                     /gene="yqeH"
                     /locus_tag="PW109_001196"
     CDS             complement(13770..14879)
                     /gene="yqeH"
                     /locus_tag="PW109_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2511033.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14876..15403)
                     /locus_tag="PW109_001197"
     CDS             complement(14876..15403)
                     /locus_tag="PW109_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2511034.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15583..16368)
                     /locus_tag="PW109_001198"
     CDS             complement(15583..16368)
                     /locus_tag="PW109_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2511035.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16361..17230)
                     /locus_tag="PW109_001199"
     CDS             complement(16361..17230)
                     /locus_tag="PW109_001199"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2511036.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17231..18610)
                     /locus_tag="PW109_001200"
     CDS             complement(17231..18610)
                     /locus_tag="PW109_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2511037.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18618..19037)
                     /gene="fabZ"
                     /locus_tag="PW109_001201"
     CDS             complement(18618..19037)
                     /gene="fabZ"
                     /locus_tag="PW109_001201"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2511038.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19062..19538)
                     /gene="accB"
                     /locus_tag="PW109_001202"
     CDS             complement(19062..19538)
                     /gene="accB"
                     /locus_tag="PW109_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2511039.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19541..20776)
                     /gene="fabF"
                     /locus_tag="PW109_001203"
     CDS             complement(19541..20776)
                     /gene="fabF"
                     /locus_tag="PW109_001203"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2511040.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20795..21532)
                     /gene="fabG"
                     /locus_tag="PW109_001204"
     CDS             complement(20795..21532)
                     /gene="fabG"
                     /locus_tag="PW109_001204"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2511041.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21544..22461)
                     /gene="fabD"
                     /locus_tag="PW109_001205"
     CDS             complement(21544..22461)
                     /gene="fabD"
                     /locus_tag="PW109_001205"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2511042.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22568..22792)
                     /locus_tag="PW109_001206"
     CDS             complement(22568..22792)
                     /locus_tag="PW109_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2511043.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22845..23807)
                     /locus_tag="PW109_001207"
     CDS             complement(22845..23807)
                     /locus_tag="PW109_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2511044.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23817..24257)
                     /locus_tag="PW109_001208"
     CDS             complement(23817..24257)
                     /locus_tag="PW109_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2511045.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6658 a         5427 c         4241 g         8018 t
ORIGIN      
        1 ccataaaata ttcttggatt gcttctcgtg tttttttcac tttttcgcta agcttttcgt
       61 ttttagccat atagaaataa aaaatgatat cgatcgtagc aaattgggcc aaaagtgaat
      121 tagttgccgc acttcgattc gtgatttctt ttggtctaga tgtttgtacg agtacatcgg
      181 ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg atattcatgc
      241 ttttcgccag ctcggctaat gcgactacat ctgcacttct acctgagttt gaaacaagcc
      301 aaagcacttt ctttttctca ttgctgacaa gttgcgggag caagacgtgg atatcttttt
      361 caaaaatgat tggcttaccg acacgagacc atttttgcag aatatcttct actactaaag
      421 aagaggcgcc aaccccatat aaaaaaacag tctctgccga ttccaaacac tctacgactt
      481 tttggatcgt ttcttcttct agaatatcta ctgtttcttt tatcgctact tgtgcattgt
      541 taccaagttt gtttttgata gagtaaaagg actcatttgc ttctatatca ctaaaacctt
      601 catcgattcc agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc agactttgaa
      661 aaccgatttt tttggaaaaa cgtacaactg tcggcgcact caacccggaa gcttctgcta
      721 attcattagc tgtcattgta gggaccttct caatattacc gataatgtag ttggccaact
      781 ttttttctgc tgaagaataa tcttccatca tactttcaat acgactaatt acgctcataa
      841 gttcctccac ccttttaatg atagtctatg taagtatacc atatcagacc cttattcacg
      901 gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa cagaatatct tatcccatca
      961 atcccacaga ttctattgag catctgtgtc tttgaggaat acctgttatc tattgttcgg
     1021 attgctttct atctactact tttttacagg aaatttacct attagttttc tctgctgtaa
     1081 tttttgactt ctgcttccat agcatcaata aagatattca aatcttctac agctgtttct
     1141 ttacttccat aacgacgtac gttcactgtt gcatcttcca attccttgtc tccaacaact
     1201 aattggtaag ggatcttttg tgtttgagag gcacgtatct tatagcccat tttttcatta
     1261 cgatcatcaa cttctacacg caaccccttc atttgcaaac gttctttgat ttcataagca
     1321 taatcgccat gtgcatcaac agaaacaggg atgattgttg cttgtatagg tgccaaccat
     1381 gttggaaaag cgcctttgta tacttctgtt aaataagcga caaaacgttc cattgtagaa
     1441 acaattccgc ggtggataac tactggacga tgcgtatttt ccccgtcttc tccaacatat
     1501 gtcaaatcaa agcgctctgg caataagaag tctaattgta tagttgataa tgtttcttct
     1561 gtaccaagag ctgttttgac ttgaacatct agttttggtc cgtaaaaggc tgcctcgcct
     1621 tctgcttcaa agtaatctag ttctaattca tccatagcgg ctttcagcat tgcttgggct
     1681 ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc gcgatagctc
     1741 aagcggaaac gataatctgt aatattgaag tcagcatata ctgccaccat caattcaagc
     1801 gtacgtttaa attcatcttt gatttgatca ggacgaacaa atgtatgacc atcattcaac
     1861 gtcatttctc gtacacgttg taaacctgac aaagcacctg atttttcata gcgatgcatc
     1921 attcctaatt cagcgatacg aattggtaat tcacgataag aatggatcgt gtttttgtag
     1981 accatcatgt gatgagggca gttcattggg cgaagaacaa gcatttcgcc atctcccata
     2041 tccattggcg ggaacatgtc ttcttgataa tgatcccagt gtccagatgt tttgtacaac
     2101 tcaacatctc ccattattgg tgtatataca tgttggtaac ctagactaac ttctttgtct
     2161 acgatataac gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca gaatggcaaa
     2221 ccagaaccta cttctgggga aaccatgaac aagtcaagtt cttttcctag tttacgatgg
     2281 tctctttctt ttgcttcttc acggagacgg atatattctt tcaaagcttt cttgtcaaaa
     2341 aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg agtttccacg ccaataagca
     2401 cctgccactg ataataattt aaatacttgt atacggccag tggatggaac atgtggtcca
     2461 cgacacaaat cgatgaattc gccttgttga taagcagtaa tcacttcttc ttctggcaat
     2521 tcactaatca gttctacttt gtacggatca cttgcaaaaa tttctaatgc ttcctgtttt
     2581 gaaagaacac ggcgttcgat cggcagattt tctttgacga ttttcatcat ttctgcttcg
     2641 attgccggca aatcttctgc agttacttgg ttttgtccat tatctgtatc gtagtaaaac
     2701 cctgaatcga tagctggtcc aacgccaaag tggatatttg gatagaggcg acgcatggct
     2761 tgggccatca aatgagctgc tgaatgacgg actaagccaa gtgcatcttc atggtctggt
     2821 gtgatgatct caatacttgc atcttcctcg attggacgag tcaaatcaac gagttttccg
     2881 ttcactttac cagctaaagc tttttttgct aagctttttg aaatgctttc agcaattgcc
     2941 aatgttgttg ttccagactc aaattctttg actgcaccat ctggaaaagt aatttttatc
     3001 ataatagttt cctccttgaa atgttttgat tgatcagatc atcaagctga aaaacaaaaa
     3061 aatcctagca tctcttgaaa agagatacta ggacgaaaag ttcgtggttc cacctaatgt
     3121 tcaaacgcaa atgcgttcct caagcgttgt aacggtccga ccgcccttgc ttcaacaagg
     3181 gtttcgaaag tggtcatcct ttggttttct cctggaaaag tttcagccta gcttttcctc
     3241 tcttatcgaa gactctcaaa ttcagttggc tttctcatca atcaattatg tgtttattta
     3301 accactaata aatagaaaaa tcaagtggga aactaaactt attttttcca ttccccattt
     3361 acaccgtatg ttcctttttt catctcattg atgatgacat ggatgtgttc ttttggtgcc
     3421 tgcgtgtttt tatgaacagc ttccgtgata tctttcatca tattttctaa ttgttccgga
     3481 ctgcgacctt ctactaattc gacatgtacg aatggcataa tgctctctcc tctttctgat
     3541 cgttcttttt tcaactcgat tctatcatac tatgtttgtc ttgggtactg aatcaggaaa
     3601 aatggctgga tctttttttg ctctcaaggc actcaaaata gagaccgtat ccacgacttc
     3661 ttgcaaaaca gctccgaata atgcggggat tagtcccgta cttgcaatta acatcaaaaa
     3721 aatacaaata aagatgccaa tcagtacaga ctgtttcgct attctcatcg tatctcttga
     3781 aatcactact gcttttgcta cacgcaagag atcatctttc aagatgaccg catctgcact
     3841 ttcactagca gctgtagaac catgagcccc cattgcaata ccgatatctg cagcagcaag
     3901 tgctggtgcg tcattcacgc cgtctcctac catcgcaaca ggtctttgat ctttagggat
     3961 actttctaaa atggtcagtt tatcttgagg caagctacgg gcatgtacag aatctaattg
     4021 gactttttct gcaatcgttt gagcaatggc agatccatcc ccggtgatca tcatcaaatt
     4081 tttcacatca agttttctta gctccttgat tgtctttgct gcttctgggc gaatctgatc
     4141 cgaaaatagg atcgttcctg caaaagatcg atcaattgtt acatagattg ctgtattgga
     4201 cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca atctttactt catgattatt
     4261 gactactgct tggattcctt gaccggtcac ttctttcagt tgactaacag gatacagttg
     4321 gctgtttttc acgttgttca ctaacgaacg agctaagata tggctagatt cctgttctgc
     4381 actagctgca tacagaagta gctcttcttt ttttattcca ttgactggct gaatcgcctc
     4441 tacttccaat atccctttgg taagcgttcc tgttttatca aaagcaatcg tttttgtacg
     4501 tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc ccgtttttac ttgaacggct
     4561 cattcctgca actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg atgccactac
     4621 cagtacctca gcaaatcgta aagggtcttt tgagaaccac caagaaatac caccgatcaa
     4681 ataagcaatc aacgtgaaag ggactgcata acgatcagct aaacggacaa atcgggcagg
     4741 cttttctttt gattcttcga ctagtttaat aatcatttga tactggctgt ctgcggcacg
     4801 tttttcagcg atcatttgga tcgatccgtc tccatttaaa gaccctgaca taatctgatc
     4861 tccaggtttc ttggtttccg ggcgggactc acctgtcaaa gaagattcat cgaccgaaga
     4921 ttctcctttg gtcactcgac catctgcggg tacaagttcg ccaggcttga ctagtaactg
     4981 gtcatcaata tttatttcat cgattactat atcctgcaat tttccttctt tgaatcgatg
     5041 ggcaattctt ggggtgttat ctaacaaaga ctgcagttct ttacccgctc gtttattcgc
     5101 gtaatcctct agactatctc cgcccgtaag catgatcaat atcatcaaac tagcccagta
     5161 ttctttgacg gctaatgttg caaggattgc ggtaatcgct aatacatcta cgccatatct
     5221 tcctgaacgt aacgtcttga tcatttccaa aaacatcaag aaggcgagaa tccctccagt
     5281 aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa acaaattcac ttaataaagc
     5341 tatcccacca ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg ttttttctcc
     5401 tcttttatgt gacttagaga ggttagtaat aaatttccag ctgcctcttt catcttaagc
     5461 atagtccttg actagcactt tttaaaacaa taggccttaa ttgaaaacaa ctatcactat
     5521 cagcgtttcc aaaattcatc agttctacaa acaaaaatat tcagcaagga ttactttttc
     5581 gattcatcag ttaaaaagta ccttttttcg aaaaactatg ctatactttt aattcgttat
     5641 ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa cgcacgtgga
     5701 tgaaacgatc gaactaatcc agatcgaaca aaatatgctg gatacaaaac aacaagaatt
     5761 gactcaggaa atgaatcatt cgacaaaaga cacagctaat cacaagattc gagctggttc
     5821 caatgaagct ttttatgaat ctgctgttga atatcgccaa catgagcagg aattgctttt
     5881 gaaataccat actcttgaag cacagcaaaa acgcctgcaa acgttgtctg tcatgaaaga
     5941 tagtccttat tttgcccgta tcgactttaa agaagaccgt gaaacagaaa cattatatct
     6001 aggtatcgct tctttaagag atctggcaga agaaacaatc gttattgact ggcgcgcacc
     6061 aattgccaat ctttattatg aaggtgagat tggtcccgca ttttatgaaa ctgaaacaga
     6121 tagaatagat gtcgatctgc agttgaaacg acaatttaaa atccaagaag gacgtatttt
     6181 atctatggta gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga tattggatga
     6241 atcatcaagc aaccatatga aaaacatcgt ctctacgata caaaaagcac aaaatgcaat
     6301 cattcgggac acaacaagta aagtaatgct gattgaagga atcgccggaa gtggaaagac
     6361 atccgctttg cttcaacgta ttgcttttct gttgtatcat aatcgcaagt ggctcgaggc
     6421 tgaaaacgtt cttctatttt cacccaacca tttgttttct gattacatct cgactgtatt
     6481 gccttcatta ggtgaaagcg gcgtgcctac acagacattc aaaaattatt tggtgcagct
     6541 gctgccagag ttttctttac tagatgaaca gcaacaagaa agtggttttt tgctagggga
     6601 aaaagatccg atacaagtga tgaaaagcgg attgacctta gtcgatcaga ttgatcgcta
     6661 tatccaaggc atcacaaact acggtccctt gttccgtgat atgaaaataa atggccgtac
     6721 gatcctatca aaggaatcga ttcgccaatg gtataaagaa acaaatgaac aattaccttt
     6781 acatcataga ctttctctat tacaaacgaa gctcctgaaa aaattaggcg gtctgcaaaa
     6841 agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc aagaactata
     6901 cgcaacagat ccaaatcttg aatatacaga acaaaaggag cgttcattac gtaaaaaact
     6961 ggccaaccaa atcgtacgta aaagattccg tcgaatccat cgagggatca tgaattatca
     7021 atttgtcaat ataacgaaac aatatctcca ctttctacaa actgtgccac tttctcttct
     7081 aacacgacat aaaatcagcg agaccaggtg gcaggaacat ttgacaactg ttcgtacaca
     7141 cttgcgtcaa agagaattgc gtcaagagga tgctgtacta ttctttttac tgatgcgccg
     7201 aacatatcca gtgacggtta cacacaaagc tcgctatatc tttattgatg aaatgcagga
     7261 tttcgcacca tcacaagtgg cattgctgca agccttgttc ccaaaagcaa atttgacttt
     7321 ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat cattggaaga
     7381 ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg acaagctatc gctccacaaa
     7441 agaaatcact gattttgcta atcattttat tgtgaaagaa catcaagtag aaacaacagc
     7501 aagaaaagga gaccttcctt tgctcatcca aggaacaaca gatgatatca aattgaattg
     7561 gttagcagat accattcgaa cgactcaggg aaaagcccgg tattggcgta cagcaatcat
     7621 agctaaaaca caagaagaat gcgaagctct ttatgctcaa ctgccagacg agttgaaaca
     7681 acaagtccag ctgattttaa atgagagtga ctttatgaag cgttccatta ttttacttcc
     7741 agccttttta gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg tagatgcaga
     7801 acatttttct tctcagcaag acaaattgat cctttacacc atgtgtacac gtgcgatgca
     7861 tgaattgacc ctttttacct ccaataatga aagtcctttg ttaaacaata tggatatttc
     7921 atcttatgag aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa tgttagcagt
     7981 tgtcttttta tttacaataa taattgcaga aaaagtaatt caatcattta aggtcattta
     8041 ttctatatat aaatccaaat ctgtttcagg atattttaat ttgacaagcg aaataatcat
     8101 tttcagttct tccatgtttt ctggatattc tgggtcaaat accttgctat gacggccaat
     8161 cccgccattg tcgatcaatc ggtaaattcg attccattca atgaattggt caattgattg
     8221 gaaattgctg attggatagc cgccacaatc caaaatctct gatacacgaa atgctaggta
     8281 tgcactatcg atcactttat tttttttcgt gtctatcttc tcatttcttt cgataagaat
     8341 tttttcagga cgatgtttca agctccactc taatagtaga aagaaagcct ccatccgata
     8401 ctcttcttca aataaccgaa aggctgaatc agaacgacga aaaagcagcg gaatcagata
     8461 attttgtcta taaatcagca ttggtgcgcg gtccaccata cactctaaat aacggttcag
     8521 ataccctgta ccgacatagt ttcctaatcg atagccttca acgccgaatc ttgcgtattt
     8581 ttgcgctaat tttgaacaat cagttacgtt cacagctaac tcttctactt tgcggtaacc
     8641 gcttaagata cgttcaaaag gttctaaagt gatttttgct tccatattct tccccgtcca
     8701 tcttccacat acttcatatc atttcacatg atacttatgc tctcatcata ccacaggttg
     8761 aattagaaaa gaaaataaat cacacaaaaa agcaagacaa tctgttagtg agattgtctt
     8821 gcttttcgaa aaagactgaa tacttttcgt taaaactggt tgcttagtct tctaatgcca
     8881 cattatggaa cacatgctgt acatcttcta gatcttccag tgcatcaatc attttttcaa
     8941 atgtttcttg atcttctgga gatagtgtta cctcgttttg agggatcatt tggatctcag
     9001 cgattgaaaa atcagtgatt cctttttctt ttaatgcttc ttgtatacta tggaaatccg
     9061 ctacttcacc gtaaacgatg atttgtcctt cttcttccat tacatcgcga acatcgatgt
     9121 ctttttccat taggtattca aggatctcgt cagcgtcttc tcctgcaaaa gcaaagattg
     9181 ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc catgttcccg ccatttttcc
     9241 caaatgctgc ccgtacgtct gctgctgttc gattcacatt gttcgtcaat gtatcaacaa
     9301 tcaccattga gccgcttggt ccaaatcctt catagcgcaa ttcagagtat gtttcatctc
     9361 ctgcaccttt tgctttttcg attgctcggt caataatatg ttttggtaca ttataagttt
     9421 tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg cggatcagga tcacctgatt
     9481 ttgctgccgc gtagatttca atcccaaatt ttgcataaat ccggctatta ttagcatctt
     9541 tagcagtttt ctttgctacg atatttgccc atttacgtcc catattttca cttcgctttc
     9601 tgatttgata gtttcataac gcaaaaacgt cctgttccat tatacttgtg aatcacgatt
     9661 ctgtgaagct ttttttcttt cttccttcat tcttctcatt tttagtcaat tatctatact
     9721 cggattggga tttgcccgaa attctgttcc ggaatattgc ctcccaattg atagatttga
     9781 tcacttctaa ctaacagttc cgcttcttgt gcctcttttt ttccgattcg ggaataaata
     9841 gaaatctctt ttttttcttt taagaatctt cttccagcat cggtaaatcc aagtagtcga
     9901 agataatttt tattttgttg gacaggaatc tcttgctttt tgatattcag caagacataa
     9961 caagataatc gttggatccg tgtccatgta tatctttttg tttttacctt tgacacaaaa
    10021 tcctcaaaac tatcagcagt tgcagctgct gttttcatgc gcgattctat tccttcggtc
    10081 atctgataga tatctgtcag ttccagcaaa gtagaagata acaaccgata acgcaaatac
    10141 ggccaataag attcccaagt ctgcagcgaa caagttgaaa gatcttcttc tgtctgaggc
    10201 ggcacaagtc cagctattct ttttccttct tctaacgcat ggcgaatggc tgttgcactg
    10261 gctatcctct gatctgtgat gattttttca tgatagcctg cacttttacg tcggatcgga
    10321 tgtaattcca tagggtggac atacttggca ttttcctttg cataacttaa tcccaagata
    10381 tgatttggcg aagaaaaatc aagcgtaata tcgggataca gactgcgaaa aacctcagtc
    10441 attttttgcg gataactata cgtttgatca gacagttctt gaaatgcctc ttctatccta
    10501 tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat caaacgaagt ctcttcttct
    10561 gttccaaaac acaaactgtc acagtccagt gtttgcaaga tttttactgc acctttagca
    10621 aaatagtcag ctgactgaac cgcccattcg acgggaagct ccactactaa atccacacca
    10681 ttttgtaaag cttcattcgc gcgatgccat ttatcgatga tagccggttc gcctctttgc
    10741 aaaaaattcc cgctcattac agcaatgacg acctctgctc ctgttatttt tcgtgccatt
    10801 tccacatgat agcgatgtcc attgtgaaaa ggattgtact ctacgataat cccgcagctt
    10861 ttcatgcgca tcctccttca tagttatcga taaaaaagaa atagagacaa gtaaaaaaag
    10921 gcaaaaatcg gaaaaaatac ccgaactata taaactattc tcgcaaacac aatacttctg
    10981 cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt cggatatata atttatgttg
    11041 aagatttatt cttttcttgt taactcgagc aggcaaaacc gctagtcttt gtatgctaca
    11101 aagaaccagc gctttgattt ctctgatgct gcttcgtctg taaaatcaga aaatgcttcg
    11161 actttcgaaa agccgctgtt ttccagcatt cttaaataac tttccaatgg ataggtacgc
    11221 tcttgatgta gttcatcttc acgaataaat cgaccagacc cttcttgttc ttcaacgaaa
    11281 aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg gataactatc ccacaaaaaa
    11341 gcaaaattat ccccttgata atgatagcta tattctggaa aaacctcatc tacttgatag
    11401 gtggagtgta cgtcaaataa aaatcggcca ttttcttcta acgcttgata gacttcatca
    11461 aacacttgct gtacctcttg tctatttttc atatagcaca gcgaatcaga aaaacaggta
    11521 attgcctgat attgtccgat ttctgataaa tcgagcatat tgccttccac aaattggatc
    11581 gatgcgtctt cttcatatgc tcgctggctg gcaatcatca acatctcttc tgaaaggtct
    11641 aaccctgtca ctttatatcc atcttttgca aaggcaaccg ctaatgctcc agtaccgcat
    11701 gccagttcca aaatattttt tgtgtcttct tgtaaatgac gtttcgaaaa agctaaccat
    11761 tgctgataaa gtgtttcgtc catcacttca tcgtaaatga aagcaaatgt ttcataagcc
    11821 atatgttaat ccaaccattc actaagatcg actagcggag catctgacca tagtttttcc
    11881 agattataga aatttcgttc tggcgcatgg aaaacatgta cgatcacatc tccaagatcg
    11941 atcaataccc attttccgcc ttcttttcct tcaatacgtt ttacttcata tttgttttct
    12001 tcttctttat caatgatttc ttctgtaatt gcgttgattt gacgttcgct atttgctgaa
    12061 cagatcatga aataatctgc caacaaggaa acatttcgaa cgtctaaagc gataatgtct
    12121 tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta attgttcttt actatctatg
    12181 atgatttcct cctaatatga gcagcttatt tttttgctac ccagtaatta tatgtttcga
    12241 ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg ggccaatgta tgttttgttt
    12301 caaatgctac agcttcgtct agatcagccc atgcaatggc tcgtgcatct tgtaccccag
    12361 gaaaatctcg acctggttca atatagtctg ctacataaat gattttgtca aggagactca
    12421 tcttagcagc cccagtcgta tgcacccgga ttgcatgaag aatctctgca tcggtgattc
    12481 ctaattcccg ctctacaaac gaagcgccaa caagaccatg ccagattgca ttattataat
    12541 tcaataactc cggatcaaaa ccgtctctta cgattaccat tttgaactcc tcatctggtc
    12601 gttcttttgc ataatcatgt gtcaatgcag caatactagc tttttcagga gaagcaccgt
    12661 attttttggc aagagccacg gctgtttctt ctactccaag cacatgcttg aagcgttgtt
    12721 cgctcattct catctgtatt ttttgcatca ggttttctct agaataagca gtatattcat
    12781 tactatagtc catattcata caacccttca ttttgaatat aatcaattac tttatctgga
    12841 acaaggtagc ggatagaaca gccttctttg atcttttgac gtattttcgt ggaactgatg
    12901 tcgatctcag gtacatctac ccaaattaca ggatatggag tatccgttgt gtatcctggt
    12961 cgacgtatac ccacaaagtt gaccatagaa gtcagctcat cgattttata ccattttggc
    13021 aaatattcta ccatatcccc accgataata aagtaataat ctgtatccgg attgttttgc
    13081 gtcaattcct tcatagtatc ataggtgtaa cttttcccac cacgagctaa ttcaatcgtt
    13141 tcaatctgca aaaaaggatt gtcttccacc gctaattcca acatgttgag tcgatgtttt
    13201 gcatcaatcg tctgtttctc atcaacatga ggtggctgat aagaaggcat caaaaatacg
    13261 cggtcaagac ctaaattccg gccagcttgt tcagccatca ctaaatgtgc aaggtgaacg
    13321 ggattgaatg ttccgcccaa gatccctact tgctttttct tctctaagaa ttgaggcatt
    13381 tcttcaggaa ataattgtga acgaacaaat gtcttagctt gcgtattcat catggtcact
    13441 ccttatattg cttttacttc ttttgatagc ctttgatact tttcttttgt ggaaggtttg
    13501 tataatacaa taacacgtcc aatgatttgg acgatttcgc aatctatttc tttttctaat
    13561 acttgagctg cttcttctgc aatctcatct gtattttgaa gtaacgatac tttgatcagt
    13621 tctctttttt ctaaagcttc ttcaatttga acgatcattg ctgaattgat cccacctttt
    13681 ccgatttgaa aaatcggctg aagatgatgc gctttgctac gtaaaaaacg cttttgtttt
    13741 cctcttaaac tcataatttt ccttcttcct taaatcaatg ctttgcgttt caatacatct
    13801 acaccctttg gcgcccagcc cgcaattact actggcgctg ttacagtgat ccagcctaga
    13861 ccggcaaaaa cgatgtctgt tttttctttc acagaaaact caaaacgaac aagctccgga
    13921 aatgcctcga cttcatcagg acgaggaggc tgtaataatc cacctacatg tttttgataa
    13981 aattcgtctg ctttttccag tttcgttcga tgaatattta catcatttga cacataagca
    14041 ataaatgaag cacgattgcc gctgataaaa tcaaatcttg caagtccgcc caagaaaagt
    14101 gtttgccctt cattcagctg ataaacttta ggtttgattt cttttaacgg tgcaatgatc
    14161 cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc gatggataat tcccggtgta
    14221 tcaatcaaaa aatgcccgtc atctaaagga atctcgattt tatccaaggt cgttcctgga
    14281 aactgcgaag ttgtgattac atcttgtact cccgctgtat ttttgatgat ttgattgata
    14341 agcgtcgatt ttccgacatt tgttactccg acgatataga catcccgtcc atcgcggtat
    14401 tcttcgattt tttctaatag ttccgtcatc tcatgagatt tcttagcact agttaataat
    14461 acatctactg gacgaattcc tgcttcgtaa gcacgttcct tcatccattg tgttaattta
    14521 gtttttttca atgatttagg taatatatcc actttgttcc caactaaaag tactggattg
    14581 tcaccgacaa aacggtgtag tcctgggatt agtgaaccat tgaagtcaaa aatatccacc
    14641 acattgacga tcaatgcatc tttttgccct aattcattca gtaagcgcaa aaagtcgtca
    14701 tccgtcaatg agacatcttg gatctcatta taatggcgta atctgaaaca acgctgacaa
    14761 tagacttccc cggaagctaa acctttttcc aaagccgttt gcggcgtgta tcctaattca
    14821 ccgggtttgt ttgtttgtat aacggcacca cagccgatac attgtaattc ttctgtcatt
    14881 cgattccacc tcgccagccc atatcagggt gttttgctaa tagataggcc ataattcttt
    14941 tttcccaaaa acgattaaat tgtgtcttcc aactatctgt tgcgataatg ggttttacaa
    15001 ggatgctacg tatccctgca cgattggctc cacgtatatc agtcataatc tgatcgccta
    15061 ccattactat ctcatcatct gctaaatcca attgtttctt tgctatgttg atccctcttg
    15121 ctaaaggttt cattgctcgt gcaacataca aaagatcaaa tttttcaatc gctcgcgcga
    15181 cacgttttga attattgttt gaaacaacaa caacagggat tcctgcattt ttcatttcta
    15241 aaatccaagt aagcaactct tctgttccat ctggattatt ccatgcaatc aacgtattgt
    15301 ctaaatcggt caatactgct ttgatgccta gattctttaa ttgggcaggt gtaatctgat
    15361 agatggcttc taccatccat gttggtttgt aatttgaaaa catgctgtct cctcatctgc
    15421 aaaaaatgaa ggagcacata aagtactcct caaccccgta ttatactgca tatcagcaaa
    15481 aaaaactacc tctatactcg ttcgtctttc agaaaaagca tttctttaca aaatgaccaa
    15541 aaataaagat tgatcacaga aaaatttaga aacaaaaaac aactaatatt ggcggaacct
    15601 cttgtatcgt ttttccagta atttatgctg atctaatcga acaagttccg tcaatctttc
    15661 aatcaacgct tttttcataa tgtgtgcgac tttatatcgt ggcaactgct cgccgttgaa
    15721 cgtttcagga ataactcgat caacgattcc taactcgcgt agttctggcg cagtaatttt
    15781 catcaactca gctgcttctg cggcgcgact accgtctttc cacaaaatcg acgcaaatcc
    15841 ttctggagaa agcacagcat aaatcgtttc ttccagcatc cagacttcat ccgctaaacc
    15901 tagagcaagg gcaccgccac ttccaccttc accaatgatg atggcgatga tcggtacctt
    15961 taaatcagac atttcgagca gatttctggc aatagcctct ccttcgccgc gctcttctgc
    16021 tcctacaccg caatatgcac ctgctgtatt gatcaaggta atcaccggtc tattgaactt
    16081 ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg gagaaccaaa
    16141 atttctgcgg atattttcct gcagatcccg tcctttttga ataccgatca ctgttaccgg
    16201 tttgccatct aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa ataaacgatc
    16261 accatggcat tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc ttgtccatcg
    16321 ttctttagct cttgctaact gaacgacatc atgtgctgtc ttatccacga tctatccatc
    16381 ctttctgtgt atgcaattca agaagcgtat ggattttctg tttcaattcc atgcggggaa
    16441 caatctgatc gacaaaacca tgagataata agaattcagc cttttggaaa tcttctggaa
    16501 gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc aagctttggg
    16561 gttctgctag aataatatcg ccttccattg caaaactggc agttactcct ccggtcgtcg
    16621 ggtccgttaa aacagttaga taaaaaagac cttcattgct atgccgtttg acagcagcag
    16681 agatttttgc catctgcatt agagaaaaga ttccttcttg catccgtgcg ccacctgaag
    16741 ctgtgaacaa gacgactgga agactttcct ttgttgctcg ttcgaataat ctggtgatct
    16801 tttctccgac aatcgttccc atacttccca tgataaaatt aggatccatc actccaataa
    16861 tgaagggaat cccctgaatg gttgctttac cagtgaggac tgcttcatga agtcctgttt
    16921 tatcttgcat ttttttaatt ttttccgtat attcgggaaa tgctagcgga tctttggtta
    16981 caagatccgt atcccattct tcaaaacttt tttcatcaat aatcaaagct agacgctgcc
    17041 atgcaccgat tcgaaaagcg tatccgcaat gagggcatac tttttcttca ccgatgtctt
    17101 tcgtataaag gatatgtttg caatttggac actttgccca catgttatca ggaacagatg
    17161 gcgagttagc aggtttgctc tctctattag gattgatccg aatatatttc tttttcttaa
    17221 acagagccat ctatgcgcct cctatctccg tttctggtgt ccaattcggt aaaaattctt
    17281 cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc aggatgacta atcaaatcca
    17341 tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag gctcgctgca
    17401 tcttcattaa tgcttcaaaa cgcgtatttc catggacgat tatttttgca atcattgaat
    17461 cataatatgg cgggatcgta tatcccgggt aaactgcact gtctactcgc agtcccatcc
    17521 cacctgctgg aagcagtaag ttttggatct tccctggaga aggagcaaaa tggaaagcag
    17581 gattttctgc attaatccgg cattcgattg cgtgtccttg gaatactact tcttcttgac
    17641 acacattcaa aggctcaccg gctgctatct ccacctgttt ttttactaaa tcgattcctg
    17701 tgaccatttc tgttactgga tgttccactt ggattcgagt attcatttcc atgaaataaa
    17761 aatcgccttc ttcatccatc agaaactcga tcgttccggc attttcataa ttgactgctt
    17821 tagctgctcg aacagcagcg tctcctagtg cttctctttt tgtttgtgag atcactacag
    17881 aaggagattc ttccagtact ttttgattgt tgcgttgtaa agaacaatct cgctcgccta
    17941 aatgaatgac atgaccaaat cgatcaccta agatctgaac ttctatatgg cgagcaggat
    18001 aaatgatttt ttccaaatac atatcatcat ttccaaatgc cgctgctgct tcttgctgag
    18061 ctgaagaaaa atgctgtggg agctcttctt ttgattgaac tttccgaatg cctttcccgc
    18121 caccacctgc agctgctttc agcatcactg gataaccaat tctgtctgct atttccaatg
    18181 cttcttctac tgtagataat acaccagtac ttcctggaat gactgggaca tttgcttctt
    18241 gcatcaattg acgcgcattg atcttatttc ccattgcatc gatcgttgca cttttaggac
    18301 caataaatgt gatattgcac tcttcacaca tggaggcaaa tcggctattt tcagataaaa
    18361 aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc agtacttcct
    18421 gaacattcag atatgaatca acggcttttg ccgggccaat gcagattgct tcatctgcta
    18481 actgtgtatg aagtgcttcg gcatctgctt ctgaatatat cgctacggtt tggatcccta
    18541 gttcgcggca tgcacgaatg atacgtacag cgatttcacc gcggttagct atcaatactt
    18601 tagaaaacat atttcaccta tccaatcata aaggttaatt ccgcttcggc tactttttta
    18661 ccgtctactt tagcgattcc tttgcctatt ccggctgaag ctttcacttt caatatctca
    18721 acttcaaggt ataaggtatc tcccggtgta actttcttac ggaattttgc tttgtccaat
    18781 ccgccgaaat aagccgtctt tcccttgaat tgttcaagag aaagtaatgc aactgctcct
    18841 gcttgagcca ttgcttcaac aatcaatacc cctggcataa caggttcttc tggaaaatgt
    18901 ccttgaaaaa agggctcatt gatcgttaca tttttcttag caacgatccg ttctccttca
    18961 accatttctt ccactcgatc aatcaaaagc attggataac gatgtggaat gatttctttt
    19021 atttcttgta tgttcattgt tttccctctt tcctgctttg cttatttttg ctgaatccta
    19081 aagagtggct gtccatattc gacaactgac tcgttttcta caaggatttc tgtgatcact
    19141 ccgtcggcct cactagttat ctcattcatc agtttcatcg cttcaataat gcagataact
    19201 tctcctttgg caacttgatc acctactttt ttgaaagctg gtttgtcggg cgctggttgc
    19261 agataaacga ttcccacgat tggcgaaaca acctcgattc catcaaatac tgccgattct
    19321 ttttctttca ttatttctgg agcagttgtt gcttgttcag atatgacatt gactggctcc
    19381 gaaactggca acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt tttattttta
    19441 ttcatataca attcaaattg accttcacgc aaatcgaatt ccgttagcga ggattgatca
    19501 aattgactga ctaattctct gatttcattg atatccaaaa ttattcctcc cagcgtttca
    19561 tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt actcattgca taagtaaaac
    19621 ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat ctcaggatct tgtttttcta
    19681 cattgatcgt aggtgggata aattgatgtt ctaatgccag caaagttgcc aaggcttcga
    19741 tcccgccggc tgctcccaat aaatgtccag tcatcgattt cgtgctgctg acgtaagtat
    19801 ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg tcattggctg
    19861 gtgtacttgt tccgtgagca ttgatatatc cgacttgttc cggctgtaca ccagcttctt
    19921 ccatcgcaag cttcattgct ttagatgcac cagaaccatc tggagtagga gctgtcatat
    19981 ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc atagattttg gcaccgcgag
    20041 cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc tccttcaccc atcacaaaac
    20101 cattccgatt ttcgtcaaac ggaatcgaac cacgattagg atcagttgat tctgagagtg
    20161 ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat cgttgcttct gttcctccag
    20221 caagaatgac atcagaatag ccatgtttga tgttacggaa tgcttcccca attgaattcg
    20281 ttccgctagc acaagctgtt acgatagctg tacaaatacc ttttgcacct acacgcaaag
    20341 caatatttcc agctgccatg ttggcaatcg ccattggtac gaacattgga gaaactcgtt
    20401 ttgcaccttt ttcatgtaga cgaataactt ggttttcgat tgttggaaga ccaccgatcc
    20461 cagatccgat gatgacgcca aaccggtctt gattcatttt ttctttatct aaaccgctca
    20521 tctccattgc ttctaaagca gcgtagattc catagatcga aaacatatcc attcgtttgc
    20581 tatctttttt aataaaatat ttatcgtaag ggaaatcttt gacttcaccc gctaaggtaa
    20641 tccctgtttc tgaagcatca aatttagtga tcggtgcgat gccattcgtc ccctctttta
    20701 agctattcaa aaagctgtct gcgtcatttc caataggtga agtgactcca tagccagtaa
    20761 ttaccactcg attcatttgt ttgctctcct tttcttatcc gtgcatgacc aatccgccat
    20821 cgacattgat gacttgtccg gtgatatatg gattttgtgc taagaaaact gctgtttgtg
    20881 caatatcttc tacttgtccg aaacgctgca tcggaatttg cttttccgct tgttccttga
    20941 ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca atggcattac
    21001 atgtaatgcc tctagttgca gcttctcgtg ccacagattt tgttaatcct actacgccag
    21061 ctttactagc tgcataattt gcttgaccaa tatttccaat caacccagat acactagaga
    21121 gattaatgat ggccccttct cgctgcttca tcattttttt caatacgtgc tgcgtcatat
    21181 tgaaggttcc aatcaaattg atatccagac attttttgaa atcttctgca tccattcgca
    21241 tcacaagttt gtcatttgtg attccggcat tgtttactaa cacatggatc gatccaagct
    21301 tttcttctgc ttctttgatc atttgtcccg ctttctcata atcagagatg tcaccggaaa
    21361 ctcctacgca ttttacaccg aatgcttcga tttcttctat cttttctttt ggaatctctc
    21421 cgcgaccatt caaaatgata tttgctccag cttttgcaaa agctaaggcc atcgctttgc
    21481 cgattcctct tgtactacct gtgataaata cattttttcc ttttacatcc ataacgttcc
    21541 tccttatagt attgctattg tttcatcaaa tgtctgctta ttctcaacac gcagaactgg
    21601 tattgtttta tcgatttttt tcataaatcc gcttaatact tttccggggc cgacttcgat
    21661 gacttgttcg atgccgattg tttttaactt atggatactc tcatagaaac gaacaggttg
    21721 catgacttgc tgttcaagta gtcccgcgat tgtttctttc ttcattattt ccgttgttgt
    21781 gttgcttatg ataggaaaag aaggttcaga gaattgaatc tgacttaggt ccttcgccag
    21841 tttttttgct gccggttcta aaatagctgt atgaaaaggc ccgctgacat ttagagggat
    21901 cattcttttg aacccttttt cttttaatag taaaacagct tcgtctactg ctttttcttc
    21961 gccaccaatc acgatctgct gaggtgtatt ataatttgca ggagatacga tgccgtactt
    22021 gctagcttca tgacagcttt cctcgatcgt ttcaatcggc gcattcatca ctgcaaccat
    22081 tttcccgctt ccagctgggg tagcttctgt catataagcg cctctttttg ctactaacgc
    22141 aactgcttca gtgaaggata gcgcattact agctactaat gccgaatatt ctcctaaact
    22201 cagtcctaaa gctgcatctg ggatgattcc atgctccttc aataagcgat aatacgcgat
    22261 gctgactgtt agaatagctg gctgtgtata ttgcgtctga tccaaacgtt cattttctgt
    22321 aaagcaaagt tcagccatat cgtagccgag aatctcactt gcttcatcaa atgtctgctt
    22381 tacaacggct tcttcatata aatcttttcc cataccttga tattgtgcac cttggccact
    22441 aaataagaat gctgttttca tgtttttctc caatatatcc agaatatatg tttgattctt
    22501 cctattcaaa aaggccgaga cattcctatt tcaggaaatg tcccagcccc caacctaatt
    22561 ttaatgatta tgcagtttgt ttttctacgt aagtcactaa atcttgaacc gttttgattc
    22621 cgtcttctgt ttcgattttc acgtcaaatt catcttcaat ttcattgatg atttggaata
    22681 aatccaagct gtcagcttct aagtcttctt ggatgtcagt agtcaatttg atttcttcag
    22741 tttcttttcc taattcttct gcgatgattt cttgaatttt ttcgaatacc ataataatat
    22801 attcctccga tattgtttgc tttttatttt tataatttaa cagtctacaa ctccatgaga
    22861 atacttcccc atgttaaacc tccgccatag cctgtaaata ttactttctg ctgttttcct
    22921 aaggtgatct ttccactaga aactgcttca tccaataaga tagggatgct tgcagcagat
    22981 gtattcccat atttatccat attggtcagg aacttttctt gcggcatctt tacttttcgg
    23041 gcaattttct caataattct gaggtttgcc tgatgcaaaa gcagataatc aactggtttg
    23101 tctttcatga cttcttggat attgtttgca acgtctcgta cggcaaaatc aaaaatatct
    23161 ctgccagtca tttgcaaata atacgaacct tctgaatctt ctgaatagaa cggattttta
    23221 ttttgctggt agccagcggt caatgagcta cttctggtac cgtcagctgc taatttttct
    23281 ttcagcaatt ttttctgtgg attcgcttct aagagaatcc cacctgctcc atcgccaaac
    23341 aacacagcag tcgtacgatc agaccaatcg attagcttag atagtgtttc accaccaatc
    23401 accagtccct tagttgagcc gcagcggatc attttttcag ccatgcttaa cgcataaaca
    23461 aatcctgagc acgcagcact gatatcaaaa gcgatagcat gagttgctcc caatttccct
    23521 tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg acattgttgc tactaaaata
    23581 aaatccaaat ctttagggtt tagatttttc tgttttatca atttttccgc cactaaaatg
    23641 cataaatcag aagtctcctg atttgttaca catcttcgtt cacgaatacc cgtccttgac
    23701 gaaatccatt catcagaggt atccatcatc ttggaaagat cattattagt aataatcttt
    23761 tcaggaagga cgctggctgt tgcagtaatc attccaaatt gttccattaa atatcctcac
    23821 ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc atgctgttcc
    23881 tccgtagcca ttccgtctaa gatgttcttg accatttcac gatgaaaatg ctgatggacc
    23941 cgaaatagta actttccttt tttcgttagt cccaacttta caacgcggcg gtcatcttca
    24001 ctgcggatac gttcgacata gccttttttt actaacttgt taatagcagt ggtcaaggtc
    24061 ccaacagtaa ttgatagttc tttggctact tctgaagttg tttttttctt gtacatcccg
    24121 attgcctcga tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga ttttttcagc
    24181 tcagactctt cgattgtcaa aatatcattg aagacactaa caagataatc atttactgtt
    24241 tttaaatctg gttccatgtt ccaccgtcca ctaattagtt tgattatcaa atcttttgat
    24301 gttcaaacta tatcttaact tttttcaaaa tgcaacctta aaag
//
LOCUS       DANKHK010000031        24264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000031 DANKHK010000000
VERSION     DANKHK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            299..1210
                     /locus_tag="PW109_001209"
     CDS             299..1210
                     /locus_tag="PW109_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2511046.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1466..2383)
                     /locus_tag="PW109_001210"
     CDS             complement(1466..2383)
                     /locus_tag="PW109_001210"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2511047.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2585..3022
                     /gene="zapA"
                     /locus_tag="PW109_001211"
     CDS             2585..3022
                     /gene="zapA"
                     /locus_tag="PW109_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2511048.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3080..3628
                     /locus_tag="PW109_001212"
     CDS             3080..3628
                     /locus_tag="PW109_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2511049.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3743..6103
                     /locus_tag="PW109_001213"
     CDS             3743..6103
                     /locus_tag="PW109_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2511050.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6190..6504
                     /gene="trxA"
                     /locus_tag="PW109_001214"
     CDS             6190..6504
                     /gene="trxA"
                     /locus_tag="PW109_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2511051.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6686..8464
                     /gene="uvrC"
                     /locus_tag="PW109_001215"
     CDS             6686..8464
                     /gene="uvrC"
                     /locus_tag="PW109_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2511052.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8609..9508
                     /locus_tag="PW109_001216"
     CDS             8609..9508
                     /locus_tag="PW109_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511053.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9501..10763
                     /locus_tag="PW109_001217"
     CDS             9501..10763
                     /locus_tag="PW109_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511054.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10924..12822
                     /locus_tag="PW109_001218"
     CDS             10924..12822
                     /locus_tag="PW109_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2511055.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13121..13840)
                     /locus_tag="PW109_001219"
     CDS             complement(13121..13840)
                     /locus_tag="PW109_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2511056.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            14177..14509
                     /locus_tag="PW109_001220"
     CDS             14177..14509
                     /locus_tag="PW109_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511057.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14496..16499
                     /locus_tag="PW109_001221"
     CDS             14496..16499
                     /locus_tag="PW109_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2511058.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16518..16988
                     /locus_tag="PW109_001222"
     CDS             16518..16988
                     /locus_tag="PW109_001222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2511059.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17009..17593
                     /locus_tag="PW109_001223"
     CDS             17009..17593
                     /locus_tag="PW109_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2511060.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17608..18594
                     /locus_tag="PW109_001224"
     CDS             17608..18594
                     /locus_tag="PW109_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2511061.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18584..18895
                     /locus_tag="PW109_001225"
     CDS             18584..18895
                     /locus_tag="PW109_001225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2511062.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKKQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18917..20698
                     /locus_tag="PW109_001226"
     CDS             18917..20698
                     /locus_tag="PW109_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2511063.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20691..22067
                     /locus_tag="PW109_001227"
     CDS             20691..22067
                     /locus_tag="PW109_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2511064.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22074..22709
                     /locus_tag="PW109_001228"
     CDS             22074..22709
                     /locus_tag="PW109_001228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2511065.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22739..24094
                     /locus_tag="PW109_001229"
     CDS             22739..24094
                     /locus_tag="PW109_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2511066.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         7982 a         4171 c         5312 g         6799 t
ORIGIN      
        1 tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg ttccaggaag
       61 tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac tatttattgg
      121 gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa
      181 atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc ttttttgcgt
      241 acttaagctg attttaagtt ttgaccctta gtctggatct acgaggtgag agtcaagaat
      301 gaaaaaaata attggcgctt ttgtatttgc aatcattatc attattatct gttttcaaat
      361 agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga acagtacggt
      421 tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca aaatcggaaa
      481 aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa ctataaacgg
      541 cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca agacaaatga
      601 tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac
      661 aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg gcggaaatga
      721 tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat taagtacagc
      781 cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga cccatactac
      841 agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa aggcagatgt
      901 ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg gaatcacgac
      961 atattattac tatggacaag aggaaacatt agcacagaca attgctgact ccttcactga
     1021 tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc gagaaaatct
     1081 tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt taaaggaatt
     1141 agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat tgaccactta
     1201 ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt tcgtttggcc
     1261 gggcttatta tcgcagatgc aagcttttga acaccgttta aggaagaggt tgtgagcctg
     1321 ccgtttaact ccgcgtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgatgact
     1381 attgtgactt gtgaccgagg agttggcatg agaacaccgt ttattcggaa caagggactg
     1441 tgacatgact tttgtcacag ttccattatt tttttagcag cttcaaagct ttttctgtat
     1501 tggcaaaatg aagtttggca tatttttcaa gcataggcat gccgcctttc tccaatagtt
     1561 ttgccgctac ttggtcagat ttcgttccag ctcctgatgg aagggtgatt ccagcttcag
     1621 ctgatgcttt gtgtaactct tccaaaaagc tggcacgact gatgatagaa gcagcagcaa
     1681 cagctaaatg atattgttct cctttagtaa cgaagtacag tttttcttgt aattgatttt
     1741 tttctaaacg cgcatatttt ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg
     1801 cttctggttt ttctggctcg atttttttta gcagcaaatg gatcgcttga ttgtgcaaag
     1861 cgactttcat ccgaacggca ttgtattttg gttggatctc attgtatttt tctggagata
     1921 agacaagcag tttatagggg atggtttttt ttaagatgtc agcaagctgg atgatttggc
     1981 tatcttttaa ctcttttgaa tctctaacac caagtttacg taattctgca agctgatcac
     2041 gtttcacata agcagcacat actgttagcg gaccaaaata gctgccattt ccgacctcat
     2101 cactacctaa aacggataat tgactgatat tttttggcaa agaagaggct gtttttgctg
     2161 gcttggcttg tttatcctgt gcctcccccc atttatcagc ttctattgct gcattcgtac
     2221 cttgaaacat cacttttccg gatgtataag cggtaatggt cgtcccgtct ttttttgcag
     2281 caaagctagt ataaggaatc tgtttattta acaaataaga gcgatagtat gctttcattt
     2341 gactaagttg gtcgtttgtt gctttgataa tgacgttgct cataaaattc tcctcgtttt
     2401 tatacataaa actataccgc aactcacgac ttttcgtttt ctaagagatt gcgagacata
     2461 gcattttttt aaagtttatc atataatagt taagtatgtg aaaaatattt gtggagaacg
     2521 gtgaaagaac caacttcatg gtacactaaa taaaactttc atcaaatagg aggaaatgat
     2581 tcgtatggcg cacgaaaaaa cacgctacaa agcagtgatt gccaaccaaa catatactat
     2641 tatcggacgg gaaaccaagc atcatatgga tatcgtaacg aagctgatca atgaacagtt
     2701 agcagaattg aaacaattat caccacaaat ggataatgaa caagcagcaa tcttgatggc
     2761 tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga atcttggagc tggaagaaga
     2821 aacagcagaa ttaaagaaaa aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg
     2881 cattgaagca atcgagaacg aagctcggga agtattgaaa gaaaatggtc aagcagatca
     2941 taccattaaa aatcatgtgg aagcacaaca gattttaaac gagaaacgaa aaggacaaat
     3001 taaacaaaaa tcttctaatt aagatccttt tatcaaaata agaaaaattt aaaaaagtta
     3061 gaagagaaag gaacgtagca tgctgagttt gctgatttta tttttattgc taattgcgtt
     3121 tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt
     3181 gtcatttatt gctgcccagc atttttacaa accattagca agtcatttgg aattatacat
     3241 tccataccca gctgtcaccc caacatcaaa gttagcattt tttgatcagg tatttgcttt
     3301 tcacctagat gaagcttttt atgcaggaac tgcttttctg attattttgc taattggctg
     3361 gctgcttacc cgatttgtgg gggtttttgt ccatggattg acgtatgtgc cgatcttgag
     3421 acaggtagac tggattgcag gaggcatcct gtcactaatc atggcttacg ttactatatt
     3481 tttgattttg cagttacttg cctttgttcc attagatgtg gtccaaaatc aattcggcga
     3541 gcatagtttc gctcgtttta tcgttgagag aacgccattt cttaccaaca agatgtatga
     3601 gctttgggta acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt
     3661 gtacagagta gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca
     3721 tagtgaaaga taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa
     3781 agtcaaacag atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga
     3841 attggtacca gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg
     3901 attgaaagtg caacgtctgc gaggcgggat ccctatacct aaacttgaaa atatccgtcc
     3961 ccatatgaaa cggatcgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc
     4021 cagagtattg tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga
     4081 aatcgaattt gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag
     4141 ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga
     4201 gttaaagagc ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga
     4261 tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa
     4321 tgaccgctac gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca
     4381 cgatcaaagt gcttcaggac agactttatt tattgaacca aaacaagtgg tggatttgaa
     4441 taatcgtttg cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga
     4501 actttctgct gaacttgttc cccatcgtca agaaatcatc cacaatgctt atgtgattgg
     4561 taaaatggat ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc
     4621 gggaatcagt gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca
     4681 ggaaaaagta gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac
     4741 aggacctaac acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat
     4801 ggggcaagca ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga
     4861 agtatttgcg gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc
     4921 tcatatgacg aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt
     4981 tgacgagctc ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt
     5041 agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa
     5101 agtttatgga tacaatcggg caaatacgat taatgccagc atggagttcg atgtggatac
     5161 actaagtcca acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat
     5221 ctctagccga ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga
     5281 tgagagccaa gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga
     5341 gaccgaatat ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt
     5401 gaaagaagct tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa
     5461 aaaagcaaac gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat
     5521 tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc
     5581 aaagacccaa ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa
     5641 gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg
     5701 ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt
     5761 aaaaatgaat gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca
     5821 acgagttatc catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct
     5881 tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat
     5941 tctagctggt tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa
     6001 gggaattact gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc
     6061 aaaccaaggc ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag
     6121 cagatggttc gctatccaag tatagaaatg ctatactaat catagatttt aaaaatggag
     6181 gtctttgata tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga
     6241 cttgtattga ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt
     6301 ttggatcagt tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg
     6361 gatgaaaatc cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg
     6421 aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt
     6481 caaatgatcg atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga
     6541 atccagttcc gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt
     6601 tgagtataat tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt
     6661 aattgaatat gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact
     6721 tcctgatcag cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg
     6781 caaagccaaa atattaaaaa atcgtgtccg ctcatatttt actggcagcc ataatacgaa
     6841 aacggaacgg cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat
     6901 cgaagcattg ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat
     6961 gttaaaggat gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt
     7021 ggtgattact cgtaaagtac taaaagacaa agcactgtat tttggaccat atcccgatgt
     7081 cggtgcggcg aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa
     7141 acctagtcag acgaaggagc cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta
     7201 ttatttcgac gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaagttttt
     7261 aaacggagga cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga
     7321 aaacatggag tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat
     7381 catgactcgc caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc
     7441 gattgacaaa ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga
     7501 acgagatgtt tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct
     7561 cggtcagttt tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac
     7621 gatcgacaag cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg
     7681 agaaaagaaa aaattagttg aattggcgaa taagaatgcc tcagttgctt tgaatgaacg
     7741 gtttgatctg attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa
     7801 cgcgatgaat atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg
     7861 gacgaatcct gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata
     7921 tcggaaatat aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt
     7981 catctatcgc agatattcac gagtgattcg cgaagggttg cctttacctg atttgatctt
     8041 gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg
     8101 aatcgatatt cctattgctg gcatggcaaa aaacgataaa cataaaacaa atgagttgct
     8161 attcggtata gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact
     8221 ccagcgaatc caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag
     8281 taaaaacagt tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa
     8341 aatgctgctt aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt
     8401 acaagaaatc ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga
     8461 atgaggaaag ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa
     8521 agagcgttta cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata
     8581 tgtgatttct tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt
     8641 tgggaactac acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg
     8701 gctaattggc caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt
     8761 gacacaagac caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga
     8821 tgtcatcggt tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca
     8881 actgctttat tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga
     8941 tttttggatg gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc
     9001 taaaggaaat cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat
     9061 cattttggat gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg
     9121 aatattggaa ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa
     9181 tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg
     9241 aaaagtacat gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt
     9301 aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa
     9361 tacgttagag attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac
     9421 tcagttcggc tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa
     9481 attgaaagca ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa
     9541 aaaatgttaa gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta
     9601 tcatttatgt agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat
     9661 tggctgataa tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt
     9721 tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg
     9781 cgtatttagt agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt
     9841 cattaggaca aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc
     9901 ttcgagcaag tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa
     9961 gtttgactcg acaaaaagtc aattttgatt caaaaggaaa aatgacagtt ggagaagata
    10021 actcgacagt ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc attattattt
    10081 tgacgtatgc gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg
    10141 aagtgatcct gtccagtacg actgcacaaa agcattttta cggaaaacta acaggtgttt
    10201 tacttgttgc attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc
    10261 aattcaaaga tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga
    10321 ttggaccatt cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg
    10381 ttttagcagc tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt
    10441 tgccagtgac ttatctatct ctaggcggat atatgcttgg attgatacta ggagcttcag
    10501 atccaaacaa tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta
    10561 tgccggttcg cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg
    10621 tacttgtagc cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg
    10681 tactcgtgta tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga
    10741 gaaatgaacg aaaaaaagca taaaatatag aaaaatatag aaggaagctg gacatttatt
    10801 gttcccagct tcttttttgt atagacctat ttctattttt ttattatatt ggatatatat
    10861 tcattgaaaa tagagcataa caactttata atgttgagta taccaatttt tggaggcgtt
    10921 ttaatggacc gctatttaga tttactttca gagaagttcc ctacaacgga agatgtcatc
    10981 acggaaatca tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg
    11041 agcgatatac atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat
    11101 cttaaagaaa agatcaaaga ccttttctct gaacaaatga cggaaacgga gatcagccaa
    11161 ttcactattt ttacggctta tccagaatat gtgttgacga ctgattggta caaaaaacaa
    11221 gataaatccc agctgatcca tcgtttgatc gatcttttac ggtttacgac agtcaaatac
    11281 actcgatcaa aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc
    11341 ttgtatatcg atgatcgtat caacgggaaa aaagactatg taaaaaaaat cattgaacaa
    11401 cttgtcttga tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa
    11461 tctgtcatcg atcatttgca tatcgtgggt gatatttttg atcgaggaac tcaagcagcc
    11521 aaagtaatgg atcgaatcat ggatttttcc tctgtagata tccagtgggg gaatcatgat
    11581 attttgtgga ttggtgcgta ttgcggctct gaggcatgtt tattgacgct tttacggatt
    11641 gccgcacgct acaactattt atttgaatta gagaaagaat atggattaaa tttacgtcca
    11701 ttattttctt ttgcccatca aacctatcag aagaatccag tatttacccc taaaaacagg
    11761 aaagatctat ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc actttctatc
    11821 ctccaattca aatcagaaca tcagttgttg aatcgacgac ctgaatttaa aatggacgat
    11881 cgtaaattgt tggaaaaaat caactatgag gaatcaacga tcgttttaga aggtcagctc
    11941 tatgagttga aagggacttg ttttcaaacg atccagccag atgatcctaa aaaattcttg
    12001 gaagaggaac aaaaagtgat cgatagtttg atgtattcat tccagcactc gttaagaatg
    12061 caaaaacaca tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat
    12121 gtactgttcc atggctgtat acctgtagat gaaaaggggc agctcttagc atttgaatta
    12181 gaccaatctt atcgcggaaa agaactgctg ggattttttg aaaaacagat aactgaaagc
    12241 gcaaaagatt tgactgcaaa tgaagatttg gcgaccgatt tgatttggta tgcgtggagc
    12301 gggccatttt cgcctttatt cggcaagagt aaaatggcaa cttttgagcg ctattttctc
    12361 acagagcctc atacgcacgt cgaaaaaagc aatccctatt atcacttgcg agatgaagag
    12421 tggttcagtg aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc agcaatcatc
    12481 aatggacaca cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa
    12541 gcatttgtta ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg gattgctggc
    12601 tactcattac tatgcaattc ttacggtttc caaatcgtga cacattatcc gttcttgcct
    12661 attgaacaac aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt gatcgagcag
    12721 cagctcccac gcaaacttaa tcggaatacg acaaaaggaa tggaattaca gcaacagatt
    12781 actcaactcc aaaggctgtt ggattatcga aaggacgatt gagaaaaatg ctagccaaca
    12841 ttgctcaata gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt
    12901 agctcaaaaa acggtaaaat attagaagct atttttctgt ttatttctct aatactcagg
    12961 tcagggcgct gatttcacga cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    13021 acaagtcaca atatccaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13081 agagttgaac ggcaggctca cgacctaatc agaaaaacga ttaaaattca ttcacaaaga
    13141 aatcgaataa acgctgttca gatgaatcta cacgatgaga aagttccgga tgccattgga
    13201 caccaaggat tttgtgcata ttatctttac tttggactgc ttcaatgagt ccatcgttgg
    13261 aaaaggcgat ggcttttaac aaaggagaaa gatctttgac tgcctgatga tgataggaat
    13321 tcacaaagta tgaatcaggg agaaggtcac ttaataaact gtccgaaacg attttgactt
    13381 cgtgagttgc aaactgcggg gcagtgggct gttgttcgtg ttttacggac cattttggat
    13441 ataatgaaag atcttgataa agtgttccct ccaacacgac attcaatagt tgaagtcctc
    13501 ggcagactgc aaaaatcggt tttctttgtt tcaatgcttc caagatcaat gcctgttcga
    13561 attggtctcg ttgaatattt gtttccgtca gttttggatg agggtcttcc atgtaaagtt
    13621 gcggactaac atcctgaccg cctgcaagaa tgagtttgtc gattttctca attagttgag
    13681 gcgctgaatc tggagacatg actggcagaa taagaggtaa tccttgagca tctaaaactg
    13741 catccacaaa tccctgcggt gtatagctta cttgatttcc ttggaaagta tctgtcgctc
    13801 gaatcagttg attcccggca atgccgatga ttggtttcat ttttattccc cctaaagtag
    13861 actaattgta acaaggattt aaaaaaattt aaactatgta agacttgccc ctatacttat
    13921 ctgttcttgc tgtgttacta tctgtaaaga gaattttcgt gtgaaattca tgacgatagt
    13981 tgaaaatatt gcttaatttg ttataataaa aagtaagcag aaataaaaag gttgggcgta
    14041 agcttcaagg atctagttct tcaaggggtg agaaggacat tttattgaag gggcctgcct
    14101 atactaagtt atctttagag gtacatactt tcaatgaaaa aagaaagcat gtacctcttt
    14161 tttaaatcga aaggaagtga agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga
    14221 agaagaaaat gagaagcgtg ctcgaaccct acggcagcag ttgaagacct atgcagaaga
    14281 aaaagagcaa gaattggaga taatccaaac gaacagtcgg caacagctga aacagttggt
    14341 agaagaaaaa gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc
    14401 agaagcagac aaggtgcaag caactttgaa agaccaatat caaaagcatc agagtcaagc
    14461 aatcggtgca atcattgaaa gggtgaaaga aacttatggc cgtcactaag atggaaaaag
    14521 tgaccctcat ttcagataaa agaaatcagg agaggatttt acaagcagta caaggaatcc
    14581 aaaatgttga aatccgggat ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat
    14641 attttccaga taccacgatt attgataaag aagcaaaatt aagcgcttta ggcaatcggc
    14701 tgaaagacat ccaagaagct atccaattca tacaacacca cggcgatagc cgtcaaaaga
    14761 aactccattt gaaacgtcaa gaactttcct tacaagactt ggaagcaaat tattccgaag
    14821 aaaatttcat gaataaatta gaagaagtcc ttgatttaaa agaaaagtgg gaacagctaa
    14881 ctgaaaaacg tgatcgttta gaagaagagg aagaatggct tcttcattgg caacagctgg
    14941 atattgctcc aagtatgtat gcaagtaaga cgaccatttt tgagatgggg actgtgagtg
    15001 cagcaaattt tgaaagtttc aaggaagaac taagtaagct tgatgacgtt tatatggaag
    15061 aaatagatta tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa aaatcagttg
    15121 ctgctataga agaaattgct gggaaatacg ggttcaataa agaaacgtat ccttatgaac
    15181 aaaatcctaa agaaaaattg caagaaacca aacagcaatt atccattgtt cacgagcagc
    15241 aaaaaaagat aacgacagcg ttaggtcagt gcagcggcta tatccaagaa tttgaatggg
    15301 tagaagaagt cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg tttatccatg
    15361 ccacttatct gatcgttttg caaggttggg tagatgcaga agaaaaacag aatttgatgc
    15421 atgtgttaaa cgaaactgta tcagaagaag atatatacgt tagtttcgaa acaccaaact
    15481 cagaagagat tcagcaggaa gtccctacta aattgaaaaa ccatccgctt atcgaacctt
    15541 ttgaattgct gactgaaatg tacagtctgc caaaatatga agaagtagat ccaacgcctt
    15601 ggatgacacc attttatctt gtcttcttcg ggatgatggt cgcagatgtt ggttacggat
    15661 tgctgatgct cattgggaca atactagctc aaaaactatt tgttttaccc cgcgggatga
    15721 aacgtttcgt aaaattcttt gagattctct cggtaccatc gatcatctgg ggattgatct
    15781 acagttcatt tttcggacag gcattaccaa aagaactatt tggtatccgc ttgcctttcc
    15841 caatcctatc tacgacggat gacgtcaata cgattttgat tttatccgtg attttcggtc
    15901 tgatacagat cttggttgga ttattcgtgg cagcaaagga aaatatcagg aggaaagatt
    15961 acttgagtgc tgtcagtgat ggttttgcct ggcaagggat cctgattggg atcgttattg
    16021 cattgattgg tgtcatgttg ataaaaaata cagcatttgt ttatcttggc gcggggctat
    16081 cgatcattgc tgctttatgt atcttaatcg tgccgattat ccaatcgccg tctaaagtca
    16141 agggtgctgc caaaggagcg tataatcttt acggattgac tggttatatc ggtgatttag
    16201 tcagttatac acgtttgatg gcactaggta tttcaggcgg aagtattggc gcggcattca
    16261 atatgctagt tgctttcatg ccgccagccg ctcgattcag tgtagggatc cttttgatca
    16321 tcgctttaca cgcactgaat atgtttttga cactgttaag tgcctatgtc catggtgcac
    16381 gattgcagta tgtcgaattt tttggaaaat tttatacagg aggcggacgt gcctttgacc
    16441 cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac aataaataat
    16501 tggaggattt ttatacaatg atggattact taattagtca aaatggtgga atggtatttg
    16561 cagtattagc gatggcaaca gcgacgattt tttcagggat tggttcagct aaaggtgttg
    16621 gaatgacagg tgaggcagca gcagcgttaa caacaagtca acccgaaaaa ttcggacagg
    16681 ctttgattct gcaattgctt ccaggtacgc aagggttgta tggattcgtt atcgcctttt
    16741 tgatttttat caacttagga aatgatatgt cagttgtaca aggattgaac tttctaggcg
    16801 cttctctgcc gattgctttt acaggtttgt tttcaggtat cgcccaaggg aaagttgcag
    16861 cagcaggtat ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg
    16921 ctgcgatggt tgaaacatat gcgattttag gtttcgttat ttccttccta ttagtattga
    16981 atgcctaagc ataaaggagg aaaagcagat gaacgccatt gaaaaaatca tctctcagat
    17041 gaatgaagca gcagagcaag aaagagcggc gttagagcaa gaagagcgaa tgaagatcga
    17101 tcaaaacttt gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa
    17161 acaaatcgaa ttacttgaaa aaaaataccg tcagcttagg aatcgccaac aagtagaagt
    17221 acgacaagaa aatttgaatg cgaagcagga atttttacga cgattgtttg cagatgcaat
    17281 agctgaaatg gagaattggg atgaatctga acaaatccag tttatcaaaa attcattgta
    17341 ttcattacct ttgacaggca aagtaacatt cattgctgga gaaaaatcag cagcttatct
    17401 ttctcagaca ttgctggatg aatggaacaa tgaacttccc tttatgatgg ttttaagtga
    17461 tgaaactgtt gctgatcaag cgggttttct gataaacgat caaggagttc agtataattt
    17521 tcttttcagc agccttgtac aagatatcca aggaacgatg agttttgaga ttgccaatca
    17581 attattcgaa taaggaggga tcagtgtatg gaataccacg aactaaatcc cttgattcgc
    17641 ggaagagagc ttgaactttt gtccaatgag acatttgaac ggatgattca aacaaattct
    17701 gtcgaagcac ttggtgaaat attgaaatcc acgatttaca gtccatatat ttatgacgga
    17761 tatgaatatg acttcgaaca gaatctttta aaggagcaga gccgtctatt tcgctggtta
    17821 aaggaatcag caccggagcc tgaaatcgtt tggatctata cgatgcgcta tactttccat
    17881 aatctgaagg ttttgacaaa agccgagatg acaaatcaaa atctggacca tttgtatatc
    17941 gacgatggat tttattcatt agaaaccttg aaggatgcca ttcatacgca agtttcaaat
    18001 gaactccctg aaagcattat ggacagtata cgagaagtac gtgattattt tgaagagtct
    18061 accattcttc agggaattga cgtgatttat gatcgacagt ttttgacaga gcagcgacgg
    18121 cttgccgata agctaggata tcaagaattg ctgacagaag tcattgcttt tattgacttg
    18181 accaatatca caacgatggc tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg
    18241 acggtacttt ccagctcggg aagtatcgaa aaagaagtat ttttgaactt tgtagataaa
    18301 gatatggaag attacacgaa ttttctatta gagacagatt atgctgattt tctgcgccct
    18361 gtcatccgtg agggaaatct tgatctcgta aagcttgaac agttgaaaga tgattatcta
    18421 agttcattgt atcaagaagc acaaacccag gctttcggac cactgccact actagccttt
    18481 ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac tggttatcgg caaaagaagc
    18541 catttcgaac cagaaacaat cagaaagcgg gtgagacaag tctatgacgc ataagatcgg
    18601 tgtagttggc gacaaagatt ctgttttgcc attcaaattg tttggctttg atgttcgtta
    18661 tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg
    18721 cgtgatttat gtcacagagc aatgtgcagc attgagccca gaaacgatcg aacgttacaa
    18781 gaaacaaatg atacctgcta tcgtattgat tcccaatcat caaggatcat tagggatagg
    18841 gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt caaaatatct tatagtatga
    18901 aaaggagata gaaattttgc aaattggaaa aatcataaaa gtctccggac ctcttgtaat
    18961 ggctgagaac atgtctgatg ccagtatcca agacatgtgt ctagtgggag atctaggagt
    19021 tatcggagaa attatcgaga tgcgtggcga tgtggcgtct attcaagtat atgaagagac
    19081 ttctggaatt ggtccaggcg aaccggttcg ctctactggt gaagctttat ctgttgaatt
    19141 aggaccaggg atcatttcac aaatgtttga cggaatccaa cggccattag atacctttat
    19201 ggaaatcaca caaagtaatt tcttagggcg cggagttcaa ttgcccgcat tggaccatga
    19261 gaaaaaatgg tggttcgaac caacagtaga agccggagaa acggtgagtg ctggagatgt
    19321 tatcgggatc gtagaagaaa caaaagttat aaaacataaa atcatggtgc caaatgggat
    19381 aaaaggtacg atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt
    19441 cattgaaacc gaaaatggtg acaaaaattt gacgatgctt caaaaatggc cagtaagacg
    19501 tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc atgatcactg gacagcgagt
    19561 gattgataca ttcttcccag tgacaaaagg aggagcagca gctgtacctg gtccttttgg
    19621 tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt
    19681 ctatgtggga tgtggcgagc gtggaaatga gatgacggat gttttaaatg aattccctga
    19741 attgattgac cctaatacag gagaatcgtt gatggaacgt acggtattga ttgctaatac
    19801 atcaaacatg ccagtagcag ctcgagaagc ttctatctat acgggaatca caattgcgga
    19861 atatttccgt gacatgggtt atgacgttgc tatcatggca gactccactt cacgatgggc
    19921 agaagcactt cgtgagatga gcggacgttt ggaagaaatg cctggtgatg aaggttatcc
    19981 agcttatctt ggttcacggc ttgcggaata ctatgaacga tctggaagag tcattgcatt
    20041 aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt gctgtatcgc cgtctggagg
    20101 ggatatttcg gaaccagtca cacaaaatac attgcgggtc gtaaaagtat tctggggtct
    20161 tgattccagt ttagcccaaa aaagacattt tccttcgatc gattggatac aaagctattc
    20221 tctttattca acagaagtag ggaagtatat ggatcatatc cttcaagctg actgggcatc
    20281 catggtcacc gagggcatgc gtattttgca ggaagaacag cagctgaatg aaatcgtgcg
    20341 tttggtaggg attgattccc tttcagataa tgaccgtctg acattagaag tggcaaaatc
    20401 cattcgtgaa gattatttgc agcaaaatgc ttttgatgat gtcgatacat tcacttcaag
    20461 agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc
    20521 gttgtcgtta ggtgcttatt taagtgagat catggacggt accgtatcga ttcgtgaaag
    20581 gatcggacga agcaaatata tcccggaaac agaattagaa aaactagatc aaattattgg
    20641 tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga ggcatgagca atgattaaag
    20701 aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg
    20761 tcaaatatga agaattaatc gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag
    20821 tattggaagt ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca agtggtatca
    20881 atttacgtga ctcttctgta cgatttttag gccatccttt agaactaggt gtatcggaag
    20941 acatgatcgg tcgagtattc gatggtttgg gacgaccgaa agataacgga ccagagatcc
    21001 tgcctgaaaa gcatttggat attaacggag aagtaatcaa tccagtagct cgggattatc
    21061 cagatgagtt tatccaaaca ggtatctctg caatcgatca tttgaataca ttagttcgtg
    21121 ggcaaaaatt accagtattt tctggctctg gtcttcctca taaagaatta gctgctcaga
    21181 tcgcgcgaca agcaacggta ctcgattcat cagatgattt cgccgtagtc tttgctgcta
    21241 ttggaatcac ttttgaagaa gcagaatttt tcatggaaga ttttcgtcaa acgggcgcaa
    21301 tcgaccgttc tgtcatgttt atgaatttag caaacgaccc tgcaatcgag cgtatcgcta
    21361 ctcctcgtat ggctttgact gctgcagaat atcttgctta tgaaaaggga atgcatgttt
    21421 tggttatcat gacagacatg accaactatg cagaggcatt gcgagagatt tctgcagcac
    21481 ggcgagaagt tccaggaaga agaggatatc ctgggtatct ttacacgaac ttagctactt
    21541 tattcgaacg agcaggaaga atccgcggac taaaaggatc ggttactcaa attccgattc
    21601 taacaatgcc agaagacgat aaaactcatc caatccctga cttgactggt tatattacag
    21661 aaggacagat catcttgact agagagcttt acaagagtgg gattcagcca ccaattgatg
    21721 tactcccgtc tctttcgcgg ttaaaagata agggaactgg acccggaaag acaagagaag
    21781 accatgcagc tacgatgaat cagctttttg cagcctatgc acaaggaaaa caggcaaaag
    21841 aattggccgt agttttaggt gaatctgctt tatctgatgt agacaagatc tatgcgaagt
    21901 ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac cgttcgatta
    21961 ctgaaacact tgatctaggg tgggaactgc tatcgatgtt gccaagaacc gaattaaaac
    22021 gaatcaaaga cgacatgctg gacaaatatc tgccagaagg gaagtgagtt ctgatggcac
    22081 gattgaatgt gaatccgacc cgcatggaat tgactcgatt gaaaaaacag ttgacaactg
    22141 ccacaagagg gcataaatta ttaaaagata agcaagatga actgatgcgt caattcatcc
    22201 tgttgattcg aaagaacaac cagctgcgtc aagaaatgga agagtctatg acagaagcaa
    22261 tgtctgattt tgtattggca aatgcttcga tcacggagcc tttcatcgaa gaattatttg
    22321 ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag
    22381 tacctgtgat gaattttgaa tatgatgaac agctgacaga cacacctttg gaatacgggt
    22441 atttgaattc cagtgccgaa ctggatcggt caatcgatcg gtttactacc ctgcttccaa
    22501 aacttttgga actgacagaa atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa
    22561 agacacgtcg gcgagtcaat gcattagaat atatgacgat ccctcaatta gaagaaacga
    22621 tctattatat tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca aggttgatca
    22681 aagtaaaaaa tatgggtacc actgactaag tggtatcctt ttttaaaaga ggaaaacgat
    22741 gattaaacaa agcaaacaaa taaagaaacg cttgccggct gttcagcttc tcgcattagg
    22801 attttttctc ctgattttag ttggcggcgg cctacttacc ctaccatttt ttagtaatag
    22861 tgggcatggg acaaatttta tcgatgcgct tttcactgca acttctgctg tctgtgtgac
    22921 gggattgaca acattgaata cagcagagca ttggaacagt gctggtcaat ttttgatcat
    22981 gcttttgatt gagatcggcg gtcttggttt tatgatggtt ccgattctat gctttgcatt
    23041 gataaagaag aaagtcagtc tcagcacacg tattatatta aaagaagcgc tgaatctaga
    23101 agaagtatca ggtgtgatga agctcatgct ttacatcctg aaattggcct ttgtgatcca
    23161 actgattggt acggttgctt tatcttttgt attcgtgcct gaatttggct ggttgaaagg
    23221 tatctggtac agtttgttcc atgcaatctc tagtttttgt aatgcagggt ttgatttgtt
    23281 aggagacagt ttagccaatc accagcaaaa tgtttatttg atcatggtgg tatctgcttt
    23341 gatcatttct ggtgggttag gcttcatcgt atggcgtgat ttactggaat atcatcagaa
    23401 aaagaagata acgattcatt caaaaatcgc attaaccgtt acaggtaccc ttttattagg
    23461 cggattcgtt gtgttctttt tcacagagcg caatggggaa acgctagtgg aagggaatat
    23521 gtttcaacga ttggcaaaca ccttttttat gagtgtgaca cctagaacgg ctggatatta
    23581 ttcagttgat tacctccata tgagccatgc aggtctgatt ttaacgatgt ttttgatgta
    23641 catcggtgga acatctgggt cgacggccgg cggattgaaa acaactacgt taggtgtgtt
    23701 gctgatccag atgcgttcga tgcttaaagg acgtacgaga gcggaagcat ttggccgtac
    23761 catccgacaa ggcgcagtgt tgcgggcact tactttattt tttgttacgt tgacgttgtg
    23821 tgtagtcgcc atcatgattt tatcggtgac tgaaacgata cctaaaactt ctggcatcga
    23881 atatattgct tttgaggtct tctctgcgtt tggtacagta ggtcttacga tgggattgac
    23941 gccagacttg actgcaatag ggaaattgat cattatagcg cttatgtaca ttggacgagt
    24001 tggtgtattg actgtggtct tctctctgat gacaaaagca caaagaacac aatcaaaata
    24061 caaatatcca gaagaaagtg tcatgattgg ataatttcat cttctgacag aaacctagtg
    24121 atttagtaaa ataagaaaat aaaataattt ttcttgcttc ttggatactt taagtgcaaa
    24181 atgttcgttt attttttgag gcactatccg ttgttgaacc tcgggtagtg cctctttctt
    24241 attgaaagga atcaataaga tttg
//
LOCUS       DANKHK010000032        24192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000032 DANKHK010000000
VERSION     DANKHK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            133..3660
                     /locus_tag="PW109_001230"
     CDS             133..3660
                     /locus_tag="PW109_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2511067.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PW109_001231"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PW109_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2511068.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PW109_001232"
     CDS             7448..7789
                     /locus_tag="PW109_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511069.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PW109_001233"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PW109_001233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2511070.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PW109_001234"
     CDS             9146..11566
                     /locus_tag="PW109_001234"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2511071.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PW109_001235"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PW109_001235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2511072.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PW109_001236"
     CDS             13000..13737
                     /locus_tag="PW109_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2511073.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PW109_001237"
     CDS             13751..14566
                     /locus_tag="PW109_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2511074.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PW109_001238"
     CDS             14579..15226
                     /locus_tag="PW109_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2511075.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PW109_001239"
     CDS             15242..15898
                     /locus_tag="PW109_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2511076.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PW109_001240"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PW109_001240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2511077.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PW109_001241"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PW109_001241"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2511078.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PW109_001242"
     CDS             18240..18758
                     /locus_tag="PW109_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2511079.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PW109_001243"
     CDS             18865..19365
                     /locus_tag="PW109_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2511080.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="PW109_001244"
     CDS             complement(19645..20565)
                     /locus_tag="PW109_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2511081.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="PW109_001245"
     CDS             20730..21743
                     /locus_tag="PW109_001245"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2511082.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PW109_001246"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PW109_001246"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2511083.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="PW109_001247"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="PW109_001247"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2511084.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="PW109_001248"
     CDS             complement(23383..24144)
                     /locus_tag="PW109_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2511085.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         8003 a         4224 c         5106 g         6859 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaaa cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gataatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgta
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aa
//
LOCUS       DANKHK010000033        23806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000033 DANKHK010000000
VERSION     DANKHK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            130..1074
                     /gene="mvk"
                     /locus_tag="PW109_001249"
     CDS             130..1074
                     /gene="mvk"
                     /locus_tag="PW109_001249"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2511086.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1067..2044
                     /gene="mvaD"
                     /locus_tag="PW109_001250"
     CDS             1067..2044
                     /gene="mvaD"
                     /locus_tag="PW109_001250"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2511087.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2057..3142
                     /locus_tag="PW109_001251"
     CDS             2057..3142
                     /locus_tag="PW109_001251"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2511088.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3139..4203
                     /locus_tag="PW109_001252"
     CDS             3139..4203
                     /locus_tag="PW109_001252"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2511089.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4452..5255)
                     /locus_tag="PW109_001253"
     CDS             complement(4452..5255)
                     /locus_tag="PW109_001253"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2511090.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5275..6117)
                     /gene="kduI"
                     /locus_tag="PW109_001254"
     CDS             complement(5275..6117)
                     /gene="kduI"
                     /locus_tag="PW109_001254"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2511091.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6133..7140)
                     /locus_tag="PW109_001255"
     CDS             complement(6133..7140)
                     /locus_tag="PW109_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2511092.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7147..7791)
                     /locus_tag="PW109_001256"
     CDS             complement(7147..7791)
                     /locus_tag="PW109_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2511093.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7891..8649)
                     /locus_tag="PW109_001257"
     CDS             complement(7891..8649)
                     /locus_tag="PW109_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2511094.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8776..9927)
                     /gene="fucO"
                     /locus_tag="PW109_001258"
     CDS             complement(8776..9927)
                     /gene="fucO"
                     /locus_tag="PW109_001258"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2511095.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10009..10320)
                     /gene="rhaM"
                     /locus_tag="PW109_001259"
     CDS             complement(10009..10320)
                     /gene="rhaM"
                     /locus_tag="PW109_001259"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2511096.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10322..11167)
                     /gene="rhaD"
                     /locus_tag="PW109_001260"
     CDS             complement(10322..11167)
                     /gene="rhaD"
                     /locus_tag="PW109_001260"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2511097.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11181..12437)
                     /gene="rhaA"
                     /locus_tag="PW109_001261"
     CDS             complement(11181..12437)
                     /gene="rhaA"
                     /locus_tag="PW109_001261"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2511098.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12434..13894)
                     /gene="rhaB"
                     /locus_tag="PW109_001262"
     CDS             complement(12434..13894)
                     /gene="rhaB"
                     /locus_tag="PW109_001262"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2511099.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14047..14916
                     /locus_tag="PW109_001263"
     CDS             14047..14916
                     /locus_tag="PW109_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2511100.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15042..15941)
                     /locus_tag="PW109_001264"
     CDS             complement(15042..15941)
                     /locus_tag="PW109_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511101.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16106..16930)
                     /locus_tag="PW109_001265"
     CDS             complement(16106..16930)
                     /locus_tag="PW109_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2511102.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(16941..18236)
                     /locus_tag="PW109_001266"
     CDS             complement(16941..18236)
                     /locus_tag="PW109_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2511103.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18252..18731)
                     /locus_tag="PW109_001267"
     CDS             complement(18252..18731)
                     /locus_tag="PW109_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2511104.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18737..19765)
                     /locus_tag="PW109_001268"
     CDS             complement(18737..19765)
                     /locus_tag="PW109_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2511105.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19782..20888)
                     /locus_tag="PW109_001269"
     CDS             complement(19782..20888)
                     /locus_tag="PW109_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2511106.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21117..23714)
                     /gene="adhE"
                     /locus_tag="PW109_001270"
                     /gene_synonym="adhC"
     CDS             complement(21117..23714)
                     /gene="adhE"
                     /locus_tag="PW109_001270"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2511107.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         6710 a         5286 c         4321 g         7489 t
ORIGIN      
        1 ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag
       61 cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg
      121 agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc
      181 gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc
      241 accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca
      301 ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta
      361 aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct
      421 gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat
      481 tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa
      541 atcgcccacg gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt
      601 tattttacaa aagggcatcc tttcgactac ttttctttga acatcgatgc ttttttgatt
      661 gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc
      721 tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag
      781 caagcaaaac aagcaatcat tgaaaattca ccagaaacgt tggcacagac aatggatgaa
      841 tcacaatcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa
      901 acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt
      961 atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat
     1021 gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa
     1081 agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact
     1141 catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga
     1201 agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca ccttgcaaga
     1261 cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct
     1321 ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc
     1381 ctctgccagc gggctagctg ctttagcagg cgcttgcaat actgctctta agcttggatt
     1441 agatgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat
     1501 tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc
     1561 agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca
     1621 gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat ccagttttta
     1681 tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac
     1741 aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac
     1801 gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga
     1861 tgcggtgcga caattgagaa agcaaggcat tccatgctac tttacgatgg atgctggccc
     1921 taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa
     1981 attatttagc aaagaacaag taattactgc acatgctggt ccaggaattg cgattattga
     2041 ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga
     2101 atatgcagtt gttgaaacag gccatccagc agttatcgct gcagtcgatc aattcgtgac
     2161 agtcactgta gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat
     2221 gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt
     2281 tcattatatc cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct
     2341 tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata
     2401 tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta
     2461 cgcgttgaat ttatctcagt tggagatttt caagattgca gcactagcca atttagcagt
     2521 tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt
     2581 ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt
     2641 acttgccctg gattggccag gtctatccat tgagccattg attgctcctg aagatttacg
     2701 tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca
     2761 ccgttcgaga gaagataaaa tggtggctta tcagcttttc ttaaaaaata gtacagaatg
     2821 tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat
     2881 tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac
     2941 gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc
     3001 tggtgcaggt gggggcgatt gcggaatcgt aattgttgac cagaaatctg gcattcttcc
     3061 tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga
     3121 tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt
     3181 catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa
     3241 atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca
     3301 ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt
     3361 gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg
     3421 aaagtccctt ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa
     3481 atcattgcca acatcggtgc agggacttct gtcgaaagag ccaaagaagc tatccgattg
     3541 tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag
     3601 ggagaccgtg attttaccaa ttggaaagta cttattcaag aaactcaaac agccattgac
     3661 gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta
     3721 gctgctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag
     3781 atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg
     3841 acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc
     3901 ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgccgtg
     3961 ggtacttcag gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata
     4021 ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat
     4081 accgcggccc tacaccaaca atctctgatc ttttccggac cagtgaagga ttggtgcgaa
     4141 gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag
     4201 taaagaacac ttttgtctca cgctctaaac acgtacaaac ggcggaagta aaagcaactc
     4261 cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt
     4321 atttcttgga gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
     4381 agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta
     4441 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
     4501 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
     4561 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
     4621 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
     4681 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
     4741 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
     4801 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga
     4861 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
     4921 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
     4981 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
     5041 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
     5101 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
     5161 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
     5221 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
     5281 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
     5341 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
     5401 aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg
     5461 gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc
     5521 atgtgtatga caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat
     5581 ttgtaattga cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt
     5641 cagtgtcagg ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt
     5701 tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc
     5761 ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt
     5821 catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc
     5881 tagttcacga cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa
     5941 ttctttttca gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa
     6001 actgattttc cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt
     6061 tgaataatga cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt
     6121 cttctactcc ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc
     6181 ttcttgacta aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt
     6241 agcaaaatca atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc
     6301 tgcaaaagca tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga
     6361 ttccacatat tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg
     6421 aacggtacgc ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg
     6481 ataataataa attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata
     6541 aagagcatcc atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac
     6601 ttcgctggct tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag
     6661 gcagccagct ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga
     6721 caaagctggg gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag
     6781 cgaccattcc aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga
     6841 ggcggcgcgt tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga
     6901 taatacctgt ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa
     6961 tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat
     7021 tgctacgttt gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg
     7081 ggttccctgc tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat
     7141 aagcagctac ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac
     7201 tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac
     7261 gacaccagca tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat
     7321 attgacttga ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc
     7381 aggaaatagt ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt
     7441 catacatcct ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc
     7501 aaaggaaggg ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc
     7561 gtctaatact gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag
     7621 ttctttgatt acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc
     7681 acctttgatg attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac
     7741 cgcaatcact ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac
     7801 ctatttccta tttagaaatt ttattttata acttggaaat ctatacccat attatacggc
     7861 agaaaagaaa aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct
     7921 ttttgtttgc ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt
     7981 cggcatactt acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat
     8041 acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt
     8101 tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa
     8161 cggatgcttt tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt
     8221 gcccatcgca gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt
     8281 ttgatgtttt ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa
     8341 gtggatagtc tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac
     8401 caaatcgatt tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata
     8461 gtttttctct gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa
     8521 gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt
     8581 ttccgctaaa tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt
     8641 attttccata taatattctc ctatttcttt ataagaaaaa ttatagcgga aataccatca
     8701 atttaaaaga gatatccaga tataaactac catttctctt tacaggcaat ttaatgttga
     8761 agatccgctt cgtttttaca tcaaactttg atataaagca atgatttctt ctactgtcgt
     8821 ttctcttgga tttcctggtg tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat
     8881 atctgcttct tttacaccta attcagaaat ggtttctggg attccaaccg ctttgctcaa
     8941 ccgatcgatc tcaccacaag ccgcttctct tacatcttct aaagacattt cagctgctcc
     9001 tttaatccct agaacaagag cgatctcacg gtacttttct ccagtatatt ctttgttata
     9061 tttcatgaca gttggcaaaa gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc
     9121 acttaacggg tgagccatcc cgtgtaccag tcctaaaccg acatttgaga agcccattcc
     9181 cgcaatatac tgtcctaatg ccattttttc tcgcccattt tgatcgccat ctacagaagc
     9241 ttccaaagaa tggccgatga tttcgattgc ttttaaatgc agcatatcag tcatttccca
     9301 agcgcctttt gtgataaacc cttcaatagc atgcgtcata gcatccatac ctgttgatgc
     9361 agccaatttt ttcggcattc ccatcatcat atcgctgtcg atgaacgcga cgattgggat
     9421 atcatgagga tcaacacaaa caaattttcg gtgatttact tcatctgtga tgacgtagtt
     9481 gatcgttact tctgctgctg ttccagaagt cgttggtata gctaatatag gtaaacatgg
     9541 atttttcgta tcagcaacac cttctaaact aatgacgtct gaaaactctg gattcgtcaa
     9601 aataatccca atggcttttg ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca
     9661 gtctgcctct gcctctttaa caaaagccac tccatctttt acattttgga tcgttggatt
     9721 aggtacgatc ccgtcgtata aagcataagc gattgctgct ttatctagaa gttctgttac
     9781 ttttgttgct ataccgtgct cgatcaatcc tttgtcagta atgacagctg ctttgccgaa
     9841 tcctcgctgc tgaaattcag ggacgatcga ctcaatcgct ccttttccaa aatacgatgt
     9901 ttcatttaaa atcattcgat tactcatttt ctattcctcc taatttgtat tatttttcac
     9961 aataaaaacg aaagaacccc aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa
    10021 gactggcatc aattcccaac tgactggaga ggcatctgga tttgtctcca tgatatcttc
    10081 catgtacttc caccattttt gattgataga tgtatttgcc attttttccc ataacttttc
    10141 atcttcgatc actaagtaac caaataacaa acttgtactc ggatcaagat agatcgaata
    10201 gtcgatgacc ccgtgttcac gaagtgctgt acgcatttct ggccaaagtt catcatgtct
    10261 ttttttgtat tcttcttgtt tttctgggta aactttcatg caaaaagctt ttttcaccat
    10321 gtcagacacc tgctttcgta tgaacttcct ccaaataacc tgcttttgga gtgacaccaa
    10381 acgcatctgc taaatgccac aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg
    10441 ctttgacgta cgtatagact tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt
    10501 catccatgtc ttttcctgac ccgtagattc catgttgtgg ccataaaacc aatcgagttt
    10561 ctttcatttt ttcagctgtt gcttccccaa tctcatttgt tcccggaacc atccacggaa
    10621 tgatggaaat tccttctgga aaaacaacta agcattccgt acacatttgc catagcgtac
    10681 gactaaagga acgttcgtct aattcatgcg taaaactcat cgctaataga tgactagcat
    10741 ggcaatgcat aacgatccga ttatctgggt ctacttctaa acgggcgata tgactcatga
    10801 aatgacttgg caactcgctt gtaggaaggc attcgttttc cagcccccac aaaagttcca
    10861 gtgttttgcc atcttcagca actcgaacga ttccaagatt ctcggcaggt tcgctcgcta
    10921 catttttaaa atatcttcct gaaccagtta caataaaata gcatcctgct aaacggctgg
    10981 catcaaaaat catcgagatg cggcggatca ctttttcagg atccaagaac ggtgtgatct
    11041 cttcttcttt tagtaaataa gaaatattcc cgccatttct ttcatcccat cccaagcgat
    11101 aaagattcga cgtcgtttcg atcatttctt tcacataagg cgcttgtaaa atatctattt
    11161 ttttcaatat agccacactc ctattttctt aattccaaca cgtctttttg atattcctgg
    11221 acttcttcca gccaatccaa accaactgga acttgatttt tgtaacagaa ataattccat
    11281 acatcggcat acgggaaatc tttcaattct tccgtataag ccattcttga agtaaagtca
    11341 aaagccagct ccatttcttt caatcgctca actggttcca gcatagcttt cagcagtgct
    11401 ttttgtgtat ttctcgtacc gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag
    11461 aaatccagac cgatatttgt tttttccaac aattcgttac ggactaattc tttagcgatt
    11521 tcctgcaatt catcatccat caaaaccaca tggtctgaat cccaacgcac tggacgggaa
    11581 acatgcaaca tgattccttt gctgaataaa gctaatgaag ataatttatt ggaaatcact
    11641 tctgttggat gaaaatgtcc agcatctaga caaacaagtt tgtcacgagt caacccgtaa
    11701 cccatataaa attcatggga accgactgta taagcttcag cgcctaaacc aaatagtttg
    11761 ctttctactg catcttgtac ataccgttca tctaactctt ctgtaaaaat ttcatcgagt
    11821 gattccatta aacgttcccg tggggtaaaa cgatcaatcg gattatcttt catcccatca
    11881 ggtacccaaa agttattgat acttgtttgc ccagtttctt tcccaaagta ctccgcgatt
    11941 ttgcggcttc ttttcccgtg ttcgatccag tattcacgaa cttctttatc tggatgagcc
    12001 aatgtaaagc cgtctttcat cattggatgc gaaaagaaag tcggattgaa gtcaagaccg
    12061 atttcttctt tttttgccca ctctacccat ttttcaaaat gtttcggctc aatttcattt
    12121 agatcgacag attcttctgt atccaaatag atagcatgaa ggttcaattt gtgtttgccc
    12181 ggtatcaacg aataagcctt ctccagatct tgtctaagct cttcaggtgt tcgtgcggct
    12241 cctggaaaat tccctgtcgc catgattcca ccggtcaatt cgccatccgg tgtcaagaaa
    12301 cctttgacat catctccttg ccagcaatgc atagaaattt tgatgtcctg tatcttttct
    12361 agtacagcat ctgtatctac accgatagtt gcatattttt cttttgcttg ttcgtaattt
    12421 ctttcaatcg tgttcatacg attactcctt tgattttatt cacgtaccat tccatcgtgt
    12481 tttgcttatt ttcatctggg taaaaagttt cacatggaaa cgactgtttg attacatttc
    12541 ttgcttgttc tatattcgaa aactgacctg tactgagcat ttgcatggca acattcccga
    12601 ttgctgtggc ttcactaggt cctgcttcta caatgattcc ggcataatct gctgtcaatt
    12661 gattcagtag acgattattc gatccgcccc ctacgatgtg cagcttttcc aatttcgttc
    12721 cagttaagtg ttctactttg atcagctctt gtgcgtaaca aagagcaaga ttatcataca
    12781 cacaacgagc gatagctccc gatgagacag ggactggctg ctctgtttca aaacagtatt
    12841 gctgaatctc ttctatcata tttgggggat tcaaaaatcg agggtcattg atttccatca
    12901 cttgctgcag cggtttttct tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt
    12961 actcttgcat tcgggcaact tcctggatca accacatccc catgatgttt ttcaaaaaac
    13021 gcaccgtgtt atgggcaccc cattcattcg tataattttc agaaaaagca ttgcgagaaa
    13081 cattggcgat ctttgtttca atccctaaaa gcgaccatgt cccactgctg atataacccc
    13141 aacgttctcc ttgacctggc gttcctaata cagctgacgc cgtatcatga cttgctactg
    13201 tgataaaggt cacatctggc aaatcatatt ttgaaaattg ttctttttgc agatctccta
    13261 gtatcgttcc cggctctgtc aatggtggaa ataattcctt cgagacaccg attttttcaa
    13321 gaagttctat ttcccattct tttgtccctg cattcagcag ctgcatagta gatgcattag
    13381 ttttctccgt caccatgtta ccagtaaaga cataccctaa ataatcaggt atcagtaaca
    13441 atttatgtgc ttttgccaaa ttttcttttt cttcagcaaa caattggaac aaggtattga
    13501 aaggctggat ctgaatccct gtctgctcat acaacttatc tagagaatag atttcagaaa
    13561 actgttctac tgctccattg gtacgcttgt cccgataagc aatcggatca tttatccgat
    13621 ccccttgttc atcaatcaga cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc
    13681 gatcaatccc tgtctgcttt aatttttcaa atccaatcaa aatctctttg ataagataat
    13741 cgatcttcca gcgatcgtga ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt
    13801 cttccaagat caaagaacct tttgcatccc tcgtactttt cattagtcgt ccgcttgatg
    13861 caccgatatc gatggcaaca aatgttggat tcatttctcc acctctttta ctttattgtt
    13921 gattagcact ttattaataa agcgttatca tttattgaca atgctatcat aatacagaat
    13981 cttatatttt gcttcacact attttggtat ataattgcat tatcttgaca agttgggagt
    14041 gtttacatgg attactggga aaaagccaac cactcatggt cggaagactc aaaaagagtg
    14101 atcttgacgc ctacacaaag aagcaggaat ttattttttt atatccaaga aatcggttac
    14161 ttcaaagcac caatccctta ctatactgaa cgagcacatt taccttctta cttgataaag
    14221 tttactttgt ctggcaaagg ttcgcttaca tataatgata atcagcaaca gctaaaagct
    14281 ggagacttat tttttattga ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa
    14341 ccatgggaaa tggattggat ccatttgaat ggtcagaacg tccaatcatt ttatcaggag
    14401 ttcacaaaag acggccataa caccttccat tcaacaggag atccatcaga aaacaagatc
    14461 caccatttga tcacagaatt gatccacatg caacaaggca cgaatgcccg taccgatttc
    14521 cagtcctctg tagctatcca cgagttgctg aatgaactga ttttgcaaaa gtatcagcta
    14581 gatttcgaag aagaggatat tcccgcctac gtttacaaac tgaagcaata cttagacgaa
    14641 catttcaaag aaacgattac acttgatcaa ttagaacaac aattcttatt gaataaatat
    14701 cagctgagca aagatttttc caaatttatc ggttctccac caatcgatta tttgatcagc
    14761 aaaaaacttt cttacgctaa agaccttctt cggtatacag aatttacggt tcgaatgatt
    14821 tccgaagaaa tagggattga gaattttgct tactttagcc gcctgttcaa agcaaaaaca
    14881 ggactatcgc cgagagactt tcgtaaattc agctgattca tataatatgt acgtcatttt
    14941 ttcaaaatat tttgaaatat atagataaaa ataaaataac cgcagtttct gcttctatgt
    15001 aaacaacagc agctactgcg gttatttttt agtgatcatt ctcattgttc atcttcaaaa
    15061 tgttcataat gaaccaactg tttctgtgta tcacgtttca aaaattcttt aggcgttatg
    15121 ccgaactctc tcttgaatgc tttcagaaaa tgcggataag aagaaaaacc acttttacta
    15181 tatacagcac tagagctgcg gtattctcgc aacaagtgct tggcatagat cagctttttc
    15241 ttcattacat actgatgaaa cgtaaatcca gtgtactttt taaattctcg tgtgatatgg
    15301 tatttgctga caaaaaactg cttttccatt tcctctaaag aaagcggatg agataaattt
    15361 tttgagatat attgtaacat ttgttccaca cgctcattag gaactacagc attctgttct
    15421 tgcgcactct cttctttttg tacaacttga ttgagaaaaa tcaagtattc taccagagct
    15481 tgttcatatt ttatgtcttc accgtaaatt tttttatcag gaggatggtc gataaatgat
    15541 aatttttgct tcaatacttc ctgttctatt tgcaatacac gactcctctt ctctcccatt
    15601 ggttgaaaac aagccgatag attagaccat ggcgtggaac gactttctag aaaagaaggg
    15661 gtgacaaaga tatagacacg ctcgaataca tctgaagatt ggcggacgat acgatgcaaa
    15721 tcattggaat ggatgagcag cacacttcct gctttgatcg taaattgtcg tccgttcaaa
    15781 taaaaagtcg cttctccctt taatgtagca tggatctcat agaaatcatg atgatgatag
    15841 atcgttttat tttggctgac gacatctttg acatgataga tttcatactg atcagaaacc
    15901 atcgtattga cttgttcacc gctgattttc ttttggagca tgtgccgcac ctccttttgt
    15961 tcacttatgc acaattttat tataccacgt tttttgtaat caaaaagagc tgcttttata
    16021 tactcttata aaaaagaaga gacgtggcat cttctagccc cgccccctcc attctaaata
    16081 ttagttaacc aacgtcttct ttacattatt caagaagact tcttccataa cgaatcccat
    16141 catcttttgt ttcttcaaga accacataca ggtacggacg attcttcatg atgatttctt
    16201 tgatttttgt gtactcgatc acaccgtctc ccaaatttac cggcacgatt ttttcctgtt
    16261 cgacacgaaa atctttcaaa tgaaccgcac atatcttttc accaaaacgt tcaaaagctt
    16321 cttctaccaa ttggacttgt tcttgataat cactacttgt gatcaaattg gtcggatcta
    16381 aaatgattcc tagatagtcc gaatctactg cttggatcag ctgctctacc cttgctaaag
    16441 aatacaccgg atgattcaaa cctggttcta tccccaccat catttggtat ttttctccag
    16501 cttttaccaa gcgacggata acggacacag cttcagcaaa tgcttcatct gtgaaatttt
    16561 cttccgtata ttggatttcc ggcaaaacac atccagtttc cgaagcaacc atcgacgcac
    16621 caaaggaaga agcatagcga agatactgct cgaactttgt cagcaactct tgtcgtatcg
    16681 ttaaatctgg atgaatcata ttgatatagc aacttaaaat tcctacggtc acttcttgct
    16741 tttctaaaac tcgtttgaca taatttccca tcccagaatt gatctcattt gcgccagaag
    16801 gtatttcagg aaacgaaaga cctaaggcga gctgcagtgt atgaatccct tgttcttttg
    16861 ttttttctgc aagatcttct atcgtttgga cttcggaaag atcatgtcct ctaattgtaa
    16921 tgttcaacat aactattctc ctaactcagc atattgggta gccacatgac gatttgcggg
    16981 aagtaagtaa tcaaaaacaa gacgactaag ataaccccat aaaaaggaag aagcggtttc
    17041 agcattgcct ctgcttttac gcctcctaca cttgcaccga catatagtcc tgtacctact
    17101 ggcggcgtga tggagcctac acacagattc atgattgaaa acaacccgta atgtacagga
    17161 tcaaccccta cacttgtgac gattggtaaa aatattggtg taaaaatcaa gatagcaggc
    17221 gccacatcca taaacatacc caccagtaat aacacaagat tgactaataa cagaataata
    17281 aatttattat ccgttacgcc cataatcaac gaactaattg cctgaggaat accggtaaac
    17341 gccatggcga aagacatcat ggaagaagta gccaataaga acatgatcgt ccctgacatt
    17401 gctactgatt gaaacaacat attaggtata tcttttagtt ttaccgtttt atagtaaaac
    17461 atagcaatca acagggagta aatgacagct atcgctgaag cttctactgc tgtaaatatc
    17521 cccgtcaaga taccaccgat aatgatgaca atcaatagaa ggctaggaat cgcctctttt
    17581 attatttttc cagcatgagc catctgtcct ttttcgacaa cagggtattt attcttttta
    17641 gcaacgatga atgctactag catgattcct agcgcccaca aagcgcctac gagataacct
    17701 cccataaaca aagcagagat cgaagcacca ccagcaacaa gtgaatacat gataaaagca
    17761 gtacttggag gaatgaccat acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac
    17821 ttccggtcat atccttcttc cacttgctga gggatcaaca ctccgccaat cgcggtagaa
    17881 gcggcaatgg ccgagctaga gatggaacca aataaagcat tccctaacac attcgtatga
    17941 gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa aatccactag tttcttagct
    18001 atccctccat tattcatgat aatgcccgag agaacgaaaa acggaatagc gatcagcgaa
    18061 aaactattga tacttcccac cattttttga gctgaactaa aagcagaaac atcaaacggt
    18121 ataactaaca ataaagtcat catcgatgag aatgcaatac ttatagcaat cggcatccct
    18181 aatacaagca acacaacaaa tacaaaaaat aacactaatc cggcttctat tgccatttta
    18241 ttcctccatt tttatatttt ttcttgaatt gctggcttgt tgcttaatgt atcgattaga
    18301 ttgatcaggc tatagataat gatgagtacc cccgatacag gtactgacag ataaacatat
    18361 cccataggaa tacttaaaga aggcgagatc tgccccattg tcaatgtgac agcattccat
    18421 ccgccataca ccatgatcat cgcagcaaat accagaaata aactttgaac aacaacttct
    18481 aaaattttac tttttttgcc agttaatttt tcacttaaaa ccgttacggc taaatgtgct
    18541 ttttgtccca caacatacgc ggaagacaac atggcaaacc agacaagacc gaatcgaaca
    18601 agctcttccg tgaccgtatt tggatttcct aaaaccgttc tggaaaatac ttgccataaa
    18661 acaacagcga ccatcccaac aagcaaaaca gctcccagga tctctaatac cttgtttaaa
    18721 atcattttca tcacagctac gctccttgct cttggatctt ttgataaagg tctttgtagg
    18781 taggatcgtt tttgaatata tcatgaagtg gggcaactgc ctctcgaaac ggtgtattat
    18841 ctacatcatt gaattggact ccatgttctt cttgtgctaa agctttttct tcttcaattg
    18901 cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt
    18961 tttcctgctc atcagttagc tgttctttga tggcatcatt catgatgatg atatctggca
    19021 cacgtgtatg ctcatcatag gaatagtatt tagccacacc accatgtcca gctgtataca
    19081 agacaaattc attgttttct gatccgttga tcaaattgga ttgaagagac gtgtacactt
    19141 catcacttcc cataggtacg ggcgaaccgc ctaaaagctc taccattttg atagcggttt
    19201 cgctttgcat cacacggatc ttctttccct tcaaatcttc cggcgtatgg atcggaccgt
    19261 ctaccatata aaagctgcgc tgaccagaat cataataggt caatccggta aatcctaaat
    19321 ttttggtcga ttggtagatt ggctccatta tttcttcatt atccatcacc ttaaagaaat
    19381 gttcttcatc attgaataga taaggaactg aaaaaatcga ataatcttta gagaaacttt
    19441 ccaacgctga ggcacttact tttgtgaaat ctactgctcc tgactgtgtc aattcgatca
    19501 gttcacgttc tccacccagt tgtccatctg ggaaatactg gatctgtacg gaaccatctg
    19561 ttttttcttt tactaaacgc ccaaattcat tcattgcatc aattactggc tggctgttgc
    19621 ttgcataagc aaagcgcaag gtaactatct catttacatc tgcatctgcg acagttgcca
    19681 cactagaatt atttccgcag ccgcttagac ctagcagacc taggctaagc gaacttgctg
    19741 caaacagcag tgatgttttt ttcatatggg tttcctttct tttaacaata agagacgagc
    19801 aattctgtca aacaaagcac cgttaacgac tgtccataag gcattgctgt gattccgata
    19861 ttccgataaa aatctaaact gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct
    19921 cctttttcat cgatttgttc aagcaatcct tggatagcac gataagcgac ttctttatat
    19981 tcttgtggta aataacgttt atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt
    20041 gcagaagatt ctacgtaaga agaaggatca tccaacaacg tatgccataa accgctttca
    20101 gactgataag tttttaatgc acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc
    20161 aatgcatctt ctttcgataa ttctaagatt tcaatgatct ctggaatagc aatcgtcaac
    20221 cagcagtttc cacgagccca aaatgcttcg gcatagttat gatttccttc aaacgtccag
    20281 ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt tgatatggat cataaactgg
    20341 taacgagctt cttctaaata ttctgggcga ttcaacagtt tcccgatttt tgccaaagga
    20401 agcactgtca tcatcaatgt atcgtcccac aactgattct tattttccgg tccatatgtg
    20461 gcatgctgta acccgtcttc atttgtccga ggcatgtcat tcatgaccca ttctgcccat
    20521 tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt catacaagta agccatagtc
    20581 aatagcggtg ccattgtatt gacgtttttg ggcggagcgc cttcttccat acgatcctca
    20641 aaccattcat tgaccacttg ataagcgcga ggattttttg tcatctggta atttttataa
    20701 ataccatata gaccgacacc ttgtggccaa ttccacactg tccagctttt gtcatctact
    20761 ttcagtccat caaagtccaa caaaaattca ccattatcgt ctttgatcgt tgtcaggttt
    20821 tcaacgagca ggtcaatttt ttctttcact aattctttag gtacaaaaat ctcttcttta
    20881 ataatcatct ttacgccacc tttttaaacg ctttcatttt atatccttaa tatagcagta
    20941 ataccgtcca ctataaatat cgctttttga acaaaaactg tctaaaattg acctaaatgt
    21001 taaataacaa aagtaacttt cagttacttg gaaattaaaa aaagaacttg gagaaacaac
    21061 gttttccaag ttctttttca tctattttct tatctttttt ctacgctttc ttttgcttat
    21121 ccgttgtaag catcaatgat gatctgtttc aattcagaga tcaatggttc tttcggatta
    21181 gctgtcgtac attggtcttc gtaagctaat tcagccatac ggtctacggt tgtatctagt
    21241 gtttcttgtg tgactccttg agcttttaga ctcatgttga tgccgacaga ttttcctaat
    21301 tcataaacag ccgttgctaa agcttcgact aattcctctg ttgtttcgcc tttcagacct
    21361 aagaattttg cgatatctgc gtaatctgta tctgcacgga aatagtcata tttagggaac
    21421 atcgcatgtt tttgtggatc ttttgcatta tagcggataa tatgcggtaa aagaatcgca
    21481 tttgtccgtc catgagggat cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa
    21541 atacctagga atgcgttagc aaacgccata ccagccattg tagaagcgtt gtgcattttt
    21601 tcacgtgatt ccatatcagg tgtttttact gatttttcta aatagtcaaa gactaacttg
    21661 attgcttgta ggctcaatcc acgtgtataa tctgaagcca tgactgaaac ataggattcg
    21721 attgcatgtg ttaacacatc catcccggta tctgctgtga cagatgctgg aactgacatg
    21781 acgaattgag gatcgacgat tgcgacatct ggcgtcaatg cataatctgc taacggatat
    21841 ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg ttacttcaga accagtccct
    21901 gatgttgtcg ggatacatac gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt
    21961 ttacggatat ccaagaattt ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag
    22021 aacatccaca ttcccttagc tgcgtccatt gcagaaccgc ctccaagagc gattactgta
    22081 tctggcttga agtctgccat cagtttcgtt cctgcgtata cggtatttgt cgatgggttc
    22141 ggttcaacat ctgagaagac ttcgatcttc acatcatttt tacgtcgtgc caatacttca
    22201 cgaactgtat cgcagtaacc gaattggacc ataccagggt cacaaacgat catgacacgt
    22261 tcaacgtttt ccattttctc taagtacaat agagagttct tttcaaagaa gatttttggc
    22321 ggtaatttga accattgcat gttatttctc cgtttcgcta cagttttgac attgattaag
    22381 tttacagcag agacgttacg agaaacagag ttcttaccat aagaaccgca tcccaaagtc
    22441 aatgacggaa tcatttcgtt atagatatca ccgatgccgc cttctgcaga tggtgtattc
    22501 actaaaatac gacaagcttt cattcgaaga ccgaatttga catgcaactc ttcgttttct
    22561 gaatggataa ctgctgtatg tcctaatcct ccaagattca acataccttc acacaaatca
    22621 aacgcatggt ccgtgttgtt agctttgatc atggcaagaa ctggagaaag tttttctctt
    22681 gatagcggat aatctgggcc tacgccttcc aattcagcaa tcagcatttt cgttcctttt
    22741 ggtacactga ttcctgccaa ttttgcgatt tccatcgcag aatgtccaac gattgatgga
    22801 ttgaccgcat attttccttc gttcatcact gcatcttcta atttttgcaa ttcatctggt
    22861 tttacgatat aaacttggtg tgcttggaat tctgctttta ctgctgcata gatttcttta
    22921 tccacgatca cagcttgttc agaagcacaa atcattccat tatcaaatgt tttcgaaacg
    22981 atcaaatcgt tcactgcacg tttaattttt gctgtttttt caatataagc tggtacgtta
    23041 cctggaccta cacctaaagc tggttttcct gtagagtaag cagacttgac cattccagct
    23101 ccacctgtcg ctaagacgat cgcgatacct ggatggttca ttagcataga agtcgcttcg
    23161 atcgaaggat gttcgatcca ttggatacaa ttttcaggag ctccggcagc gatagcagca
    23221 tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg cacttggatg gaaagcaaaa
    23281 acgattggat ttcttgtttt caacgcgatc attgatttga aaatcgttgt ggaagtcgga
    23341 ttcgttgttg gagttactcc gcaaaccaca ccgactggtt cagcaatttc aatcaaccct
    23401 ttttggacat cttcattgat cacaccaact gttttatcat gtttgatatt atgccaaata
    23461 tattcggacg catacatatt tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc
    23521 tctacagcca tcttcgctaa tggcatatgt tgatccaaag ctgccattgc catttcatgc
    23581 acgatatggt ctactttttc ttgatcaaag ctttccattt ctttcaatgc tgcatttgct
    23641 ttttctgcca aatcatcaat cattgcttgt acgtcaattg tcttcgtttt ttctttttct
    23701 attactttag ccatcattat cctcctaatg ggtcatggtt atttgttaaa taattcacat
    23761 gtatatcata caatatttat ttttctatgt aaacactttt ttgtga
//
LOCUS       DANKHK010000034        23573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000034 DANKHK010000000
VERSION     DANKHK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            299..1960
                     /locus_tag="PW109_001271"
     CDS             299..1960
                     /locus_tag="PW109_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2511108.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="PW109_001272"
     CDS             complement(2030..2377)
                     /locus_tag="PW109_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2511109.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="PW109_001273"
     CDS             2461..3402
                     /locus_tag="PW109_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511110.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="PW109_001274"
     CDS             3403..4446
                     /locus_tag="PW109_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511111.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="PW109_001275"
     CDS             4452..5522
                     /locus_tag="PW109_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511112.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="PW109_001276"
     CDS             5512..6465
                     /locus_tag="PW109_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511113.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="PW109_001277"
     CDS             6477..7487
                     /locus_tag="PW109_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2511114.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="PW109_001278"
     CDS             complement(7772..8593)
                     /locus_tag="PW109_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2511115.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="PW109_001279"
     CDS             complement(8690..8968)
                     /locus_tag="PW109_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511116.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="PW109_001280"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="PW109_001280"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2511117.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="PW109_001281"
     CDS             complement(11724..12311)
                     /locus_tag="PW109_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511118.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="PW109_001282"
     CDS             12486..13808
                     /locus_tag="PW109_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2511119.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="PW109_001283"
     CDS             13963..14739
                     /locus_tag="PW109_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2511120.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="PW109_001284"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="PW109_001284"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2511121.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="PW109_001285"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="PW109_001285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2511122.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="PW109_001286"
     CDS             16629..17258
                     /locus_tag="PW109_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511123.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PW109_001287"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PW109_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2511124.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="PW109_001288"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="PW109_001288"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2511125.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="PW109_001289"
     CDS             19445..19981
                     /locus_tag="PW109_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2511126.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="PW109_001290"
     CDS             complement(20021..21784)
                     /locus_tag="PW109_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511127.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="PW109_001291"
     CDS             complement(21787..23502)
                     /locus_tag="PW109_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511128.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7590 a         4314 c         4787 g         6882 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttattatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agctatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa att
//
LOCUS       DANKHK010000035        23552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000035 DANKHK010000000
VERSION     DANKHK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            61..627
                     /locus_tag="PW109_001292"
     CDS             61..627
                     /locus_tag="PW109_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2511129.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            772..859
                     /locus_tag="PW109_001293"
     tRNA            772..859
                     /locus_tag="PW109_001293"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:804..806,aa:Ser,seq:cga)
     gene            1195..2169
                     /gene="bsh"
                     /locus_tag="PW109_001294"
     CDS             1195..2169
                     /gene="bsh"
                     /locus_tag="PW109_001294"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2511130.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2494..4389
                     /gene="asnB"
                     /locus_tag="PW109_001295"
     CDS             2494..4389
                     /gene="asnB"
                     /locus_tag="PW109_001295"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2511131.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4584..5030)
                     /locus_tag="PW109_001296"
     CDS             complement(4584..5030)
                     /locus_tag="PW109_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2511132.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5258..5407
                     /gene="rpmG"
                     /locus_tag="PW109_001297"
     CDS             5258..5407
                     /gene="rpmG"
                     /locus_tag="PW109_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2511133.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5495..6013
                     /locus_tag="PW109_001298"
     CDS             5495..6013
                     /locus_tag="PW109_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2511134.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6090..7046)
                     /locus_tag="PW109_001299"
     CDS             complement(6090..7046)
                     /locus_tag="PW109_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2511135.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7212..8018
                     /locus_tag="PW109_001300"
     CDS             7212..8018
                     /locus_tag="PW109_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511136.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8015..9004
                     /locus_tag="PW109_001301"
     CDS             8015..9004
                     /locus_tag="PW109_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2511137.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTWLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9001..10005
                     /locus_tag="PW109_001302"
     CDS             9001..10005
                     /locus_tag="PW109_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2511138.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10137..10679
                     /locus_tag="PW109_001303"
     CDS             10137..10679
                     /locus_tag="PW109_001303"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2511139.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10699..11397
                     /locus_tag="PW109_001304"
     CDS             10699..11397
                     /locus_tag="PW109_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2511140.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11418..11630
                     /locus_tag="PW109_001305"
     CDS             11418..11630
                     /locus_tag="PW109_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2511141.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11630..12592
                     /locus_tag="PW109_001306"
     CDS             11630..12592
                     /locus_tag="PW109_001306"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2511142.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12610..13005
                     /locus_tag="PW109_001307"
     CDS             12610..13005
                     /locus_tag="PW109_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2511143.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13168..13533
                     /locus_tag="PW109_001308"
     CDS             13168..13533
                     /locus_tag="PW109_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2511144.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13530..15341
                     /locus_tag="PW109_001309"
     CDS             13530..15341
                     /locus_tag="PW109_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2511145.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15403..15570)
                     /locus_tag="PW109_001310"
     CDS             complement(15403..15570)
                     /locus_tag="PW109_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2511146.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            15819..16808
                     /gene="galE"
                     /locus_tag="PW109_001311"
     CDS             15819..16808
                     /gene="galE"
                     /locus_tag="PW109_001311"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2511147.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            16820..18304
                     /gene="galT"
                     /locus_tag="PW109_001312"
     CDS             16820..18304
                     /gene="galT"
                     /locus_tag="PW109_001312"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2511148.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            18328..19308
                     /locus_tag="PW109_001313"
     CDS             18328..19308
                     /locus_tag="PW109_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2511149.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            19499..20221
                     /gene="tsaB"
                     /locus_tag="PW109_001314"
     CDS             19499..20221
                     /gene="tsaB"
                     /locus_tag="PW109_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2511150.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            20205..20747
                     /gene="rimI"
                     /locus_tag="PW109_001315"
     CDS             20205..20747
                     /gene="rimI"
                     /locus_tag="PW109_001315"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2511151.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            20754..21218
                     /gene="rimI"
                     /locus_tag="PW109_001316"
     CDS             20754..21218
                     /gene="rimI"
                     /locus_tag="PW109_001316"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2511152.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            21215..22231
                     /gene="tsaD"
                     /locus_tag="PW109_001317"
     CDS             21215..22231
                     /gene="tsaD"
                     /locus_tag="PW109_001317"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2511153.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(22803..23504)
                     /locus_tag="PW109_001318"
     CDS             complement(22803..23504)
                     /locus_tag="PW109_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2511154.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         7451 a         4027 c         4974 g         7100 t
ORIGIN      
        1 tgctaaaaat agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta
       61 atgagaagaa aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca
      121 aaggaagggt ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca
      181 cagccgattt atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct
      241 atctttgaac aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt
      301 gatctcggtt tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg
      361 tacattgatg aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt
      421 gaaatagtta aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg
      481 catactggta catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg
      541 catccaacag aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca
      601 ttagataatc ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc
      661 gttggctgca aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct
      721 attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg
      781 gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt
      841 tcaaatcctg taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc
      901 aagtaataag aacggccaaa caaaaatagc ttttcctatt ttttcgtttg gttgggctta
      961 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact
     1021 tttgtcacag ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag
     1081 tactattttt tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaaaaa
     1141 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggaa aacgatgtgt
     1201 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
     1261 tcttataatg aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta
     1321 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
     1381 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
     1441 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
     1501 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
     1561 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
     1621 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
     1681 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
     1741 aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg
     1801 aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct
     1861 cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa
     1921 agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat
     1981 actggagaag gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg
     2041 atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat aaataaagaa
     2101 gacttggatc gtcatgaatt ggtcagttat ccagtcataa aagagcaaca gataaactat
     2161 gtcaattaat taatgaatag atccgtttgg tatcatattt tctgatacag tcataattag
     2221 acacgatctt taggtcgtgt ctttttttgc tgtttttttt ggaaatagca tccactaata
     2281 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
     2341 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
     2401 gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata
     2461 aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac
     2521 gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga
     2581 cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag aagattaagt
     2641 attattgact tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt
     2701 atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc
     2761 cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga
     2821 acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag
     2881 aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg
     2941 aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac
     3001 aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac
     3061 ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa
     3121 gatggaaaac tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat
     3181 tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat
     3241 acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta
     3301 acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat
     3361 gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg
     3421 atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct
     3481 gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg
     3541 cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga
     3601 ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa
     3661 ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaatttcca tggacggatc
     3721 catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc
     3781 gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat
     3841 gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg
     3901 caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt
     3961 tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg
     4021 gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga
     4081 caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct
     4141 gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc
     4201 gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac
     4261 catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca
     4321 tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg
     4381 actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg
     4441 agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg
     4501 gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt
     4561 cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt
     4621 ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct
     4681 tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat
     4741 tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc acgaggattc
     4801 gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat
     4861 tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta
     4921 tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat
     4981 ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat attagtatcc
     5041 ccctttatta aaaaacataa tctatataaa caagtgtaag ggtatttata caaaaagaaa
     5101 aagattttgt ttgagaagta aaaccgttgt atttatcgtc aagtagtttt acttaacaaa
     5161 atcgtgaaaa acactagcaa tctagaaaaa atcatggtaa gataatcaag tctgagagtc
     5221 atcttagagg attcttgtaa aaatgggagg gaaaaacatg cgcgtaaaca tcactttaga
     5281 gtgtacttct tgtaaagaac gtaactatct aacaagcaaa aacaaacgta acaatcctga
     5341 tcgtttggaa aaacaaaaat attgcccacg cgaacgcaaa gttactttgc accgtgaaac
     5401 aaaataatgt ttttaaagtt ctcaattcct tgctggtcaa agaattgaga ctttttttat
     5461 ctcaaaacat ctaaaatcat aaggagcgga taatatgttt atttccatgt gggcacaaga
     5521 taaaaatggt ctgatcggca aaaatggttt gttaccatgg cgattgccaa atgatatgcg
     5581 tttctttcgc gaacatacaa tggatcgaat tttagtgatg ggaagaaaga cctatgaagg
     5641 aatgggggat ctttcacttc cttatcgcca tatcatcgtg ttgacaacgc aaccaaattt
     5701 tcgcacgaaa gaaaatgcgg aagtaatgca ttcgatcgat gaacttttgt cgtatagtca
     5761 aacgatctca gaagatatct atgtttcggg tggtagtcgg atttttcaag aacttcttcc
     5821 ttacactggt cggatctggc gaacattgat cgatagcacg tttgaaggcg atacgtatat
     5881 tgggaatatt gatttttcag acttcgccct tatagaggag tatgagggga taacggatca
     5941 agaaaatcta tatgcccatc gttttcaaaa atgggaaaga gtaaaaaata gggttgcttc
     6001 aaaagaagaa taaaatctat gcgtctagtt tgttaataag aaaaaaagca tctcacgact
     6061 aatcgtgaag atgctttttt tagttaaaat caatttagtt tttctttgat atcattgacc
     6121 atttcctgac tggcaatagg tccgttatat agccaactag attcaggtcc gaattgcagt
     6181 acatgattgt ctgtcactgc tttaagattc ttccagcttg gttcattgaa catggcagca
     6241 tctttgtcac tgttcacaag gatgatgtag tcagcatcta attcagctag cttttcagag
     6301 gaaacagcag accagtcaga agtggctttt ttggagacct cttccactag atcaggaaca
     6361 ccaaattcca gatcgtcgta tacgattctt ccacttgacc gatttttgct gaccataaag
     6421 gctgagttgt tggtcaccca caacactgca gctgtcttgt tctcgatttt atctgctagt
     6481 tcttctcgag ttttcttgat ggatttttga taatcggaaa tgattttttc tgcctctttt
     6541 tcttttgcca aagctttgcc gacatctttt agttgttctt cccatgtgac atctgttccg
     6601 ttttttacaa cataagttgg cgcgattttg ctatattgtt cgtatttccc gccttcgaca
     6661 gtagcggaag aagaaatcaa cagcaaatca ggctcaaagc ttaacacttt ctcataagga
     6721 aggtcataag aaatcgttgg gacatccttt aaatcatctt gcagataatc ttgaatggaa
     6781 ccactgccaa cagtccactg ggcgataggt ttttccccta gtgcaatcag atagtcttct
     6841 aagtaactgc cgatgatccg tttgggcgcg tttggaatct ctactttatg tccaagtgta
     6901 tctgtgactg tatgagtact attggatgaa gtcgtcgaat tggcagttgt agctgtttct
     6961 ttatctgaag agggggagca agcgctcagt actccagcca gcagcaatat gctagataat
     7021 gtgaatactt ttgtttttaa tttcatttct ttcctcctaa cataattgag aatctttctc
     7081 attggttatt attcctgata tttcgaacgc tgtcaaccgc ttcatttggc tatagatatg
     7141 aaagtagcgc ttgacgaaat gttgaaacag cgtcataatt gagaatgatt ttcagcgaaa
     7201 ggatgggtga aatgacgaga ttaagagcag ctgacattgc agccggctac gaaaacaaaa
     7261 aaatcattga aaacatgtct gttacgattc ctgaagggaa aatcactagt ttgattgggc
     7321 caaatggaag tggaaaatct actttattga aaagttttac ccgtatacta cctgtgaaat
     7381 caggaaagat cattgtcaat aactctgata tctctacata cagttcaaaa gaacttgcaa
     7441 aaaaaattac attattagca caaactagtg aacaccctac aggtatgaca gtagaagaag
     7501 tagtatctta cggacgatac ccttatcaaa aactgttcag tggcatggac acgcaagatc
     7561 atgaagcaat cgagtgggcg ttggatgcta caggtctagc tggatttcgt atgcgggata
     7621 tcgcaacgct gtctggtgga caaacccagc gtgtctggat cgctatggcg cttgctcagg
     7681 aaacagatat cttgatcttg gatgaaccga caacattttt agatccggca caccaattag
     7741 agatcctaca gttattgact gaaatcaatc aaatgaaaca aacaacgatc ttgatgtcga
     7801 ttcatgacat taatcatgct tcacgttttt cagattattt attagcaatg aaaaacggta
     7861 agttgatcac agaaggtaca cctgaagaag tgatcacaaa agagtgtatg aaaaaaatat
     7921 ttgagattga tgcaacgatt gtcacgcttc caagaacaaa taaaccagtt tttctaacct
     7981 atgatttgtt gccaaaagag caggaggatc aattatgagg actatgaaga aaaatagact
     8041 cctgcttttg gtctgttttg ttcttttgat tatttttttt gttgctcttt caaatggcgc
     8101 agcgactgtg ggaaaagcag atttttggca ggcgttgttt gattttgata gatcaaaaca
     8161 aagtcaccaa atcatacgaa atattcgttt gcctcgtgtg gcgggagcat ttcttgtagg
     8221 gagttgtttt gcattgagcg gtgcattgat gcaaggagtg acaagaaatc ctttagcaga
     8281 ttcaggctta ctaggaatca acgcgggtgc ttcgttggca atggccctta gttttgcttt
     8341 ttggcctaaa gcttctgatt tttctgtgtt tctaatgtca ttggcaggtt cttttgctgt
     8401 aactttgctc gtatttggtt ttttgcagtt ttcgaaaatg ggcatgaatc ctttacagct
     8461 gattttagct ggcgtagcta ttagttcttt ttttactgct atcagccaag cacttaccca
     8521 attctttaat ctgcagcagg atcttgcttt ttggtttgta ggcggagctg ccaatatcac
     8581 atggaaacaa ttagcagtat tgtttccttt atatatactt gctgttttgg tttctttttc
     8641 attaggaaaa gcaatatctt tgattcattt gagtgaaaca catgcattag ctcttggaag
     8701 tcatcctaaa cgaacgtggt tgattgtttt ctgtctagtt gcagtgttag cagccattgc
     8761 cgtttcgtta gtcggacctg tttcatttat tgggctgttg gttccgccag ttgttcgcgg
     8821 aatggttgga acggactatc gttatgttct acctgtctct tttttagttg gcggtggatt
     8881 ggttatgttg gcggatttta cagcgaggat gatcaatccc ccttttgaaa caccttttgg
     8941 attagtcatt gcattgattg gcgttccgtt tttactgata caagtaagga gggaacaacg
     9001 gtgaaaaaaa tatattggac gttctatttc ttgctttttg tacttgtcat cctgtctata
     9061 ggtagtttaa tgattggtac gccttttatt tacctggatc agctttttgc cacgttcata
     9121 gggaaagaat catcttcttt ggcgctgatt gttagagaat ttcgagttcc tcgattactg
     9181 atcagtttat tagcaggagc ttgtcttggc gtttctggtt atatcttgca aggagtaaca
     9241 agaaatgaat tggcagattc tagtatattg ggcattaatg ccggagcagg atttttagtg
     9301 atgctttatt taggtttctt ttcgcactat tcgctgccgt ttctattgct agttgtagcc
     9361 tttctaggtg gtgttttagc tgcttgctgt gtctatcttg cggcctatga tcgaaatggc
     9421 tttttgggta tgaatcgaat cttattgtcc ggtattgccg ttaatgccgg cctttctgca
     9481 ttaacattgc tatgcacgat ccaattatct aaggaaaatt atggatttgt gaatgcatgg
     9541 ttagcgggat caatctgggg tgcgagctgg ccttatgttt gggcattgct tccatgggcg
     9601 gttatcttaa ttccgctagg aggactttat gcacagagga ttggattgct atcatttggt
     9661 caagaacgcg cgcaaggatt aggccttaac gtggctaaaa gtcaaaaaat ccttttgttg
     9721 ttagctgttg ctttggcaag cggtagtgta gcggtgacag gtagtttatc ttttgtagga
     9781 ctactagctc ctcatgctgc gaagctagtc gtacgtaaag aaaataactg gacctttgtt
     9841 ctgagcggtt tgttcggttc catttttctg cttagtgcag atatggttgc tagagtgatt
     9901 ctaccaagtg gggaagtacc tacaggaatt ttgattgccc tttttggtgc gccatatttt
     9961 ctgtatgcac tttttatcag acaaaaacac gcgctgaaca gttgaaattt attaaaaaag
    10021 gaaggccaat catttctagg tcttcttttt attctattta tgcatttatt tgaaagttcg
    10081 attgctattt ttcttttttt cctataccat tttatgagag aaggaagggt gagtttttgg
    10141 accaattgaa agtaagatta cgccggaaag gaatcgaaaa gctgaaaaat ctggccaaaa
    10201 gtggcaagaa aaaggaaaaa gaacagcgaa tctataaaca gctattcgcc agcgagtact
    10261 ggaagtcagc aaaagtgata gggacgactt tatcgaatga atttgaactg gatacacagc
    10321 cggttatcca acgagcagaa aaagaacaaa agaaaatagt tgtacctaaa actttgccga
    10381 aacgtcagat ggcgttttat gaaatggatg aacagtccgt tcttgtacgt tcgagttttg
    10441 gtgttcttga gccacaatca gagaagctct atttgccgga gatgatcgat ctcctcattg
    10501 taccaggtat tatctacaat agagccggtt atcgaatcgg ttttggcggt ggttattacg
    10561 accggtattt aaataatttt cataaaaaaa catgtagcct tgtttttcgt gaacaattga
    10621 atgaacaatg gcaaccggag agctttgatc agaagatcca acaattgttt attgactaaa
    10681 atgaaatggg aggttttcat gaattatcaa cagcaaataa aattgcgtgc atggttaaga
    10741 cgaccgttat ggacctatgc ttttttaggg atacagacag cggtcttcct catgatggaa
    10801 ttatttccgc gacttgagat cccttattat gccggaatgt atggtcctta tcttgttcac
    10861 ttcaatgaat ggtggcgttt agtgacacca atcttcatcc attttggact gatgcatttc
    10921 gtaatgaact cactgatact gtattttatg gggcagcaaa tcgaggcaat ctatggccat
    10981 tggcgttttt tcttgattta tatgttcagc gggataatgg gaaatacagc aagctttgct
    11041 tttaacgaag cgaatgttct gtctggtggt gcgagtactt cgatattcgg tttgttcggt
    11101 gcgttgttta tcttagggtt tcattttaaa cataacccag cgatccagca gctagtcaga
    11161 cattttcttt tatttattgt gttgacattt gtttttggtc ttctggatac ctctgtagat
    11221 gtttggggac atgtcggtgg gctaattggc ggattagtgt tagggaatgt tttgggtcta
    11281 ccgaaacaac agacaagcta ttcgatacac caaaggattc tttctacact agtttttgta
    11341 tttctttttg taatatgtat tttactgggt ttaaaaaaat atggtttact tgtataatat
    11401 gaaggaagtg cttaaagatg gagagcttat atgatgtaca gcagctgttc aaaaaatttg
    11461 gcatctttat ctatgtgggt gcacgcatct atgacattga attaatgact atcgaattgc
    11521 gtaatctgta tgatagccgt ttgattgatc atgatacata catgacagcg tggcgcatat
    11581 taaaaagaga gcaccgaatt gaagaaagcc gaaagaaagg aaatttgtaa tggacaagaa
    11641 gattattggg attgatttag gtggaacaac agcaaaattt gcaattttaa caccagaagg
    11701 agagattcaa caaaaatgga gcatcgatac caatattttg gatgatggta agcatatcgt
    11761 tcctgaaatc attgaatcaa tcaaccatcg tttgaattta tataatatga aagccgaaga
    11821 ctttatcgga atcggcatgg gtaccccagg aagcgtggac agcgaagctg gaacggttat
    11881 tggtgcgtat aacttaaact ggacagaagt tcaatttgtg aaaaaactga tcgaggcagg
    11941 aacaggaatc aaattcgcta tcgataatga tgcgaatgtt gctgctttag gtgaacgatg
    12001 gaaaggtgct ggagaaaacg atccagacgt tgtattcgtt actctaggaa caggtgttgg
    12061 cggtggtatc gttgctggcg gaaatttgat tcacggcgtg agcggagcag gcggagagat
    12121 cggccatatc actgtggatc ctgaagggtt cgaatgtact tgcggtaaac gcggatgttt
    12181 agaaactgtg tctagcgcta ctggtgtggt acgtctaggt cgttacttgg cagaagaata
    12241 cgcaggagat tcaaaactga aagcaatgtt agacaacggt gaagaagtaa caagtaaaga
    12301 tatctttgaa atggctcaag aagacgatcc gtttgcattg atggttgttg atcgcgtatg
    12361 cttctactta ggtttagctt gcgccaactt ggggaataca ttgaatccgt caagtattgt
    12421 gttaggtgga ggagtatcgg cagcaggaga atttttacgt agccgtgtag agaaatattt
    12481 taaagaattt actttcccgc aagtaagaga atcaacaaaa atcaaattag cagagcttgg
    12541 taatgaagct ggtgtcattg gtgctgcctc attagccttg aaatttgctt aaaaaaacgg
    12601 aggcattaca tggttttatg ggtaattaat atcattttgc tattgattgt gcttgcaatc
    12661 atcttttggg aagtctatct acgagtaatg gccaagcgtt cagcaacgac tttgacagaa
    12721 gaagaatttc gagaaggaat gagaaaagca caagtaatcg atgttcgcga aaaagatgtg
    12781 tttgatgctg ggcatatttt aggtgcaaga aacatccctt acactgtttt gaaagattca
    12841 ttaggatcta ttcgaaaaga ccagcctgtc tatatttacg atcaaaagaa aagcttgagc
    12901 atccgtacag caaataaatt acgtaaagca ggttatcaag agatttattt acttaagggt
    12961 ggatatgacg gatggtctgg aaaaatcaaa agtaaaaaag tataaaacag atgattgtat
    13021 gacagcttta aaaaccgaat caatcattag attttgacca agatcacatg aacggcgcaa
    13081 tggatagttg tgccgttcat tttttttatt ttgtgctatt atctacatat ataagctaag
    13141 cgaaaaggca gaaagaagga aacaactatg atcaaattaa aacgtgttta ccttgatgct
    13201 gaaaaagagg atggctatag aatactcgtg gataggttct ggccacgagg aatttcaaaa
    13261 gaaaaagcgc agttagatct gtggttgaaa gaaattgcac ctagtaatga agcacgcaaa
    13321 acttttgacc atgtaccaga acgatttgct tcatttgcaa agaaatacca gcaagaattg
    13381 attttcgaag aagaaaagaa gcaagctatt tttaaactca gtcaacttat gaaagaacat
    13441 ccagtcatta cttttgttta tggtgcaaaa aatgaacatc agaaccatgc tgttttattg
    13501 aaaaaattta tcgaaatgga ggcatgcaaa tgaaaatagg gatcattggt gccggaccta
    13561 gaggattaag tatagcagaa aggattctcc ataataatca ggacagacat tctcttgaac
    13621 tttttctttt cgatcccaat ggtcctggag gtcgagtttg gcggttgaat cagccaccgg
    13681 agttattaat gaattctgtt tctcaacaag tgacgctttt tacagatgaa acattgtcca
    13741 gtggaggatt ggtaacacag ggacccaact tgtttcaatg gagtaaagga cctgctacag
    13801 attatatcga acagacggta accaaaaata agcaactaat tatagaagaa atcaatcgtc
    13861 tggaaaaaga tcagcaatct actcgatgtt tatacggact ctatcaacgc tggtttttct
    13921 caaagttgca agaagaattt ccagcttcta ttcagtttgt ttattcttta gtaaaaaaag
    13981 tcagcaaaag aaaagatggt tatcttttac agacagatca gcaaagctat tctatcgatc
    14041 agcttgtttt agcaagcggt cactgggaaa atgatgagac agaagaagaa agagtgtggg
    14101 tagactacgc aaagaaaaat gatttactgt atcagcttcc ttctaatcct gcagatgtcg
    14161 atctttcgaa gataccgcct aaagaaaatg tctttttaag aggtctaggc ttaagttttt
    14221 ttgattatgt cggattgttt acagtatcaa gagaagggcg cttcgagaac gaaaaaggaa
    14281 aacttgtcta tcatccatca ggcaaggaac cgattgtata cagtggatca agaagggggc
    14341 tcccttatta ccctcgagga agaaaccaaa agcaaggcgg atcaatggca tggccacggt
    14401 tactaacaaa agaaaacctg gagcaatggc atcagtcggg atttttgact ggcgaaacat
    14461 tttttgacta tctgaaaaaa gacgttgagt tgttttatta caagaaaatc atcgaagaat
    14521 atcatttgcc gattccaaag aaaaattttg agcatgattt tctctccctc tcttcagagg
    14581 agtgtctcgg aaagcatcca gcactaatcc cttatagatg ggattggtcg tatttagaaa
    14641 ttcctcttac ctatcaggat gagtcctttg cagaagctag ccgagcattt attgaagggc
    14701 agataaaaga agcggaaaaa ggcaattgca caggagcatt tgcttcaaca tttgatgcat
    14761 taaaagactg gcgtgatcca atccggcagg ctattgaatg gcacgttttt tctgcaaagg
    14821 aatacaaaga aatactatgg ggatggttta ctcatctaaa tagcttttta acgatcggtc
    14881 cgccagtcat tcggacgaaa gaattagcag cactgataga tgcgggtatt tttcatttgt
    14941 tcgaaccgcc atttgatatt cagcaaaagc acaaccgcta tgttatttct ctacaagata
    15001 aagaaatcag cagtcgctat ttgattgaag ctcgattgcc acaaacaagc ttttctcgtt
    15061 caagtaatcc tgtcataaaa aatttgaagg aaaacaaact tgttcgccct ttttcatttt
    15121 cagataatac aggaagttat cattcagaag ctatagatgt tgagcgaggt acgaatcgcg
    15181 tgattgatgc ttcaggggaa atccagtctg acttttattg ctatggaata cctgttgaag
    15241 gtgtggattg gctaactgca gcagtagcta gaccttatac agatccatgg aatctgagac
    15301 aggcagataa aatagcacaa cttatattga ctgacaaata aaaatacgca tgatgactta
    15361 tctgttcttt cacaagtctt catgcgtgtt tttaaataat tattaacgag aaacgcgttt
    15421 gcgcattgct tttttacggt tttcttcgat cattgcttct ttttcgtcaa ttggatcgat
    15481 cactgttttt ttcacagaag cgaccaaacc agcaacggca gcggctgttg ccagacttcc
    15541 tactaatatt ccagatacaa attttttcat tgaatccacc tcatttatta tttgttatat
    15601 ctattatgga acaaaagaga aaaaaaggct agttttacgt ttgtttgtaa acctttgcaa
    15661 aaaatccttg aaaatgttta ctaaatgttt tattataaat ttaccatatg aatttacgaa
    15721 attttacttt gtttgttttc accataaaag taaacttcgt ttttttaaat tatatataat
    15781 caacaaacac aattttacta atcaaaggag tgttttttat gacaatttta gtactaggcg
    15841 gagccggtta tatcggttcg cacgctgtag accagctagt tcaaaagggg tatcaagtag
    15901 ctgtggtaga taatttgctc acaggtcaca aacaagcagt acatccagat gctcattttt
    15961 atgaaggaga catccgggat aaagaatttc tacgttccgt atttgaaaag gaaccgattg
    16021 aaggagtaat ccattttgct gcaagctctt tagtaggaga atctgtcgaa aaaccattga
    16081 tgtatttcaa caacaacgta tacggtatgc aaatcttact tgaagtcatg cacgagttca
    16141 atgtgaataa aatcgtgttc tcttctactg cagcaactta cggagaacca aaagagtctc
    16201 caatcacaga agatacacca gctaatccta aaaatccgta tggtgaaagt aaattgatga
    16261 tggaaaaaat gatgaagtgg tgtgaccaag catatggcat gcgctatgtc gctttgcgct
    16321 acttcaatgt agccggagca aaagcagatg catctatcgg agaagatcat acaccagaaa
    16381 cgcatcttgt cccaatcatt ttacaagtag ctcttggtca aagaaaagca ttggctgttt
    16441 atggagacga ttatgataca ccagatggaa catgtatcag agattatgtt caagtagaag
    16501 atttgattgc tgcacatatt ttagctttgg aatacttgaa agaaggaaat gaaagcaact
    16561 tcttcaattt aggcagcagc aaaggatact cagtcaagga aatgctagaa gcagctagag
    16621 aagtgacagg aaaagaaatt ccagcagaaa tagcaccaag aagagctgga gatcctagcc
    16681 gattagtcgc ttcaagtgaa aaagcgagag aggttctggg ttggaaacct gaatacacag
    16741 atatcaaagc aattatcaaa acagcatggg attggcatgt cagccatcca aatggttatg
    16801 aagaataggg gaatgagata tgtcaatcag tcaaacgatt tgtgattttg ccacacttgc
    16861 gatcgaagct ggcggatgga tggaaatgga ccgcttatat ttacagaatc gtcttttagg
    16921 gatggtagga gaagatgctc tagaaagcat tgaagtaaga ccagttaagg aagaatctgt
    16981 agacttattg gatcagcttg tcgctaaggc aagagaaaat ggtgttcttt cagaattgcc
    17041 agcagaacaa gagatttttg aagcgcaatt aatggatttt ctcacaccgc caccttcagt
    17101 cgtcaatgca ttctttgcac agcactattc gaaaagccca gaggaagcaa ctgagtattt
    17161 ctttaattta agtaagaaaa atgactacat caaaacaaga gccatcgcaa aaaacatcaa
    17221 atttccggca gagtcggagt atggtgaact ggaaatcaca atcaatttat ctaagccaga
    17281 aaaagatcca aaacaaatcg cagcagaacg cgaggcggta aaagtagact atcctacctg
    17341 catgctttgc atggaaaacg aaggatatag aggtcgcttg aactatccag cccgcaccaa
    17401 tcaccggatt attcgaatga atctagacgg tgaaagctgg ggattccaat attctcctta
    17461 tgcttattac aatgaacatt cgatcatttt atcagaacaa caccgcccga tgaaaatcac
    17521 aaaggaaaca tttgctagac tgcttcggat tactgaagtc ttgcctcatt actttgttgg
    17581 ttcgaatgct gacttgccga ttgttggagg atcgattctt tctcatgatc attatcaggc
    17641 aggacgtcat gaatttccta tggcaaaagc accgatagaa aaacttatca aactagcaga
    17701 ttatccgcaa gtcatcgcag gagtcgtgaa atggccgatg tctgtgatcc gtttgcaatc
    17761 aacagagaaa aaattgttga ttgaagcagc taactcagta ttggaaaagt ggaaagcata
    17821 ttcggatgaa acagtatcta tcagagcttt ttcagcagat ggtacaccac accatacgat
    17881 tacgccaatt gcacgaagaa gggacaatta ctttgagttg gaccttgtcc ttcgagacaa
    17941 caatgtttca gaagaacatc ctgatgggat ttttcatcca cataaagatg tccaacatat
    18001 caaaaaggag aacattgggt tgatcgaagt aatggggctt gctgttttac cacctcgttt
    18061 gaaaccagaa ctagtagaag tagagcattt cttattagga gaagaaaacc agatggctga
    18121 atatcatcgt ccgtgggcag aacaattaaa aaaagaacat cctgatttga cgaaagatac
    18181 ggtcaccgat gtcgtacaaa aatctgtagg tcaggttttt gcacgtgtac tggaagacgc
    18241 tggagtattc aagcgagatg aaaaaggaca ggcagcgttt gaacgattta ctgcaatttt
    18301 ataaacgtaa aaaaggagga gagacaaatg gcaacaatta aagacattgc gcagttggca
    18361 ggagtttctc ctgctactgt ttcaagagtt ctcaactatg atcctgatct ttccgttggc
    18421 ttagccacaa agcaaaaaat ttttgaagca gcagaacgct taaattatac gaaacatcgt
    18481 aaaaatgcag caaatactaa gcaaacgctt cgcctgatcc agtggtacga cgatacggaa
    18541 gagcttgaag atctttatta tttatctatt cgtctaggaa tcgaaaaaaa ggcagaagaa
    18601 gcaaacgtta ctttattgaa agaaacgtgg gattccatta gtgatcttcc gacagatgga
    18661 acgatcgcac taggaaaatt cgacgaagct cagttattag aaatgaaagg atcccaaaat
    18721 gccttgcttt tggtagattc tgacggcact tctcaaggaa tcgactctct agttgtcgac
    18781 tttaagtcaa gtgttcaaaa agtcattgaa cattttgtct cccataaagc gcaacatatc
    18841 gctatgcttg ctggaacaga atatacgaag aaaaaccatc agcggctaaa agatcccaga
    18901 attatggcat ttcaacaagt tttggcagaa aaaggctacg acgaaggtcc aattatcgaa
    18961 gcagattttt ctgtcgagtc aggatatcaa gcagtgaagc agtatttaaa aacgaatccg
    19021 catgttcctg atgcgctttt tgcagctaat gatgcattag cgattggcgc actgaaagcg
    19081 attcaagaaa aaggtttagt ggtaccagaa gatatttctg tgatgggttt caatgatgtc
    19141 agtgtagcga aatatgtaac gccctctctt tctactgtaa aagtttatac cgaatggatg
    19201 ggagagctag cagtggagac gattcttcaa ttaagtaatt ccaaagcacc ggtttcaaga
    19261 aagattattg tggaaacaga gttgattatt cgagaatcaa cgaaataaaa gatggaaaag
    19321 ctgtggggaa atgaagtgat accgcagctt ttttgtgttt caggaagggg caaaatttgc
    19381 ccgccagcag ataatcatgt atcattgtac tagttttgcc tttggtattt tcatcctcag
    19441 aataaaaaag tatgctacaa tggaaggcga gaatgtcata gcagaaagga tcaaatatat
    19501 gattacatta ggaatagata ctgcaaacca aactttggcg atcggtgtgg tagaagacga
    19561 acaaattctt ggacaaatac aaacaaatat caaacgcaac cacagtgtaa cactgatgcc
    19621 tgcaatcgac caattgtttg cagatcttca aataagtcca aaagacattg accgaatcgc
    19681 ggtttctgat ggtcccggtt cttatacagg gcttcgtata ggtgtaacga cagcaaaaac
    19741 gattgcttat acattagata aagagctagt aggagtatct agtctgaaaa ccatcgcggc
    19801 taattgcatc ggtgtggata aaattattgt gcccatgttt gatgctcgaa gaaaaaatgt
    19861 atacgctggg gcataccgct ggaaagaaaa tagattagaa attgtacttg aagatgtaca
    19921 tatttctgct tctgatttgt tcacacaact acaaaagctg aatgaagcta tttatttcgt
    19981 cgggagtgat tgtcacaaat tcttctatga aataaaggaa atattgccta gtgcgatgat
    20041 taatacagtt ccagcatggg atattccaag tggtgtaact gtggctcaat taggtgcttt
    20101 agctgaacca gttgcagaga aacaagcttt tcttcctcgc tatttaaaac gggtagaagc
    20161 agaagaaaaa tggttggaga cgcacacacc tggagacgaa agttatgttg aaaaaattta
    20221 gagcgtatgc cagaagcttt tttcaagata aaatgattta tacagaaaaa acaatcgaac
    20281 aaatcgatcg tcgttacgta ctaagagaat tgactacaga agatatcaaa gagctgctgg
    20341 atgtcgaacg agaagtctat aatggcgaat tgccatggac aaaaagtgcc tttatatcag
    20401 aattgaagtc accgcttcct catctatatc taggtatctt tgataatgga agaatgattg
    20461 gttttatcgg ttcaagagcg ttcggtttag attgccatat cacgaatatt gccgttcgac
    20521 cggcatatca gcgtaaacga atcggttcgc tgcttattga cgaaatcgag aactttgcta
    20581 taatgaatcg ctgtgagacg atgtcattag aagtgcgtat gagtaatcaa gatgcacaaa
    20641 gactttatcg gaagttaggt tttcaagcaa caacgatcaa aaaaagatat tacactgaaa
    20701 caaaagaaga tgcgttggat atgataaaac ttttagaaaa tgagtgagat tagttgattt
    20761 gcagaaaaaa agattttgat gaagcgtact tggcagaatg cgtatggatg attagcgaaa
    20821 atgcttatcc acatggttca ccttggacga aaatgcagtt tttggcagac atccaacagc
    20881 ctcactctga ttatctgctt ttagttgaag ataaccagat ctgtggcttt attggatact
    20941 cgaaagtaat agatgaagtc gagatcacaa atattgctgt tgcagtttca gaacaaggaa
    21001 aagggcatgc acggcatttg ttacaattac tgattacgga acaaataaaa gattcgctgc
    21061 atgttttttt agaagtacgt ctatctaatg aagcagcaag aaaactttat gaatcagaaa
    21121 agtttcgtat attaggtaaa cggaaatcat attatcgcaa tccgacggaa gatgcgatca
    21181 ttatgagcag aaagttaaaa acggagacga tgaaatgaat gaagaattga ttctggcgat
    21241 tgaatcaagt tgtgatgaaa cgagtgtagc ggtcgttaga aatggaacag agatcttatc
    21301 taacatcgtt gcttctcaaa taaacagtca taaacgtttt ggtggcgtag ttcctgaagt
    21361 agctagccgc catcacgtag aacaaatcac cttatgcttg gaagatgcac tggtagaagc
    21421 tggtgtgtcg gcagaagatt tatcggcagt cgctgtcact tatggaccag gacttgtcgg
    21481 ctcgttgctg attggtattt ctgcagctaa ggcttttgct tgggcacacc aattgccgct
    21541 tattcctgta aaccatatgg cagggcatat ctatgcagcc cgtctggtta aaccattcca
    21601 gtttccattg atggcacttc ttgttagcgg aggacataca gaattagtct atatgcaaga
    21661 agatggatca tatgaaatca ttggggagac aagagatgac gcagcaggag aagcttatga
    21721 caaagttgga agagtattag gtctttctta cccaagcgga aaagaaatcg atcagctagc
    21781 tcatcaggga aaagataatt accattttcc acgagcgatg atccatgaag ataattatga
    21841 tttcagtttt agcggattaa aaagtgcatt tatcaatttg gtgcataatg cacaacagcg
    21901 tggagaagat ctagacaaaa atgatttagc agctagcttt caagcaagtg tgatcgatgt
    21961 attgattact aaaacacttc gagcttgtca aaattatcca gtcaagcaac tgattgtagc
    22021 tggaggtgta gcggccaatc aagggttgcg tgaaggattg caggatgctt tggcaactaa
    22081 actccctgag gtagaactag tgattccccc tcttcgactt tgtggagata atgctgcaat
    22141 gattggagca gcagcacatg tggaaatgca aaaaagaaat tttgctagct atcagttgaa
    22201 tgctgatcca agcttggttt tatctgaata aagaggttgt gaaaaatctg ccctgcatca
    22261 agtaataaga acagcccagg aaaaacggct tctcatgttt ttgacctggc tgttcttatt
    22321 accgcgtatg caagcttttg aacgccgttt aatcaagagt tcgtaagcct gtcgtttaac
    22381 tctgattcac aaggaacaat ttcaaaaaac agctcctcat gtttatgaac attatgactt
    22441 gtgaccgaag agttgacagg tacacaccgt ttaatcgaaa taagggctgt gacacgcttt
    22501 tgtcaaagcc ctatttttta cagcttattc atcattatcc gttcctctga ttgccctcac
    22561 tgattaatcg tttattgtcg aggaatatcg aataatctag gtcggatgag gacaagacgc
    22621 ttcctctagt taatctatca gtattttttg caaacagtga atattacata actgaaacac
    22681 acatagaaaa tctttgagtc ttgcccagtg atctaaaact gccggatctt tcagaagttt
    22741 tagggaggaa aataagcagt gaccctgata aatatcaggg tcactgctta tttttgttcg
    22801 cgttattctc tcatagctgg agtagtagct gtcttacaga aagtaaaacg ctttttgtca
    22861 taatctaaca gtccggaatc acgtagttgt ttgattactt gtccagcagt ttcacgtgtc
    22921 gtgccgctat tcactgctaa ttcttttaag gtgatcggat aaggaatggt gatatgacca
    22981 tcttttgttg gtacgcccaa ttcttctttc aaaatgaata aattttgttt tacccggtca
    23041 aaggcgctag aggtcaatcc gatttggata cgcaattcat gtatttcaac tactttagaa
    23101 attttttgta tcatatagat caactgttcg ggattttttc gtgctaattt ttcatagtag
    23161 gaaacaggaa ggtagaatgt ttcaatatca gtcatcgctt gagctgtata agtgtaggtc
    23221 tcttctccaa acattcctga cataggaaaa aggttatttg tatttacata gtcatagtag
    23281 taataagttg cactttcatc ataacgttct aaacgaacga gtcctttggt caagatatac
    23341 aaccgttgtc tgggatcacc atcgtcgaaa aggatctgtc cttttttata agaacgaaga
    23401 acagtatgtt ccttgatctg ttggaactct tcatccgaga aatgttcaaa atcaggttga
    23461 tatctgagtt ttgaaaagtg aaaatcgatt ttattaacac gcattaaaaa gtcctctcct
    23521 tattgttcac tatatttatt gtcacttttt tt
//
LOCUS       DANKHK010000036        23525 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000036 DANKHK010000000
VERSION     DANKHK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..384
                     /locus_tag="PW109_001319"
     CDS             <1..384
                     /locus_tag="PW109_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511155.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            398..799
                     /locus_tag="PW109_001320"
     CDS             398..799
                     /locus_tag="PW109_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511156.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            801..1172
                     /locus_tag="PW109_001321"
     CDS             801..1172
                     /locus_tag="PW109_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511157.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1208..1510)
                     /locus_tag="PW109_001322"
     CDS             complement(1208..1510)
                     /locus_tag="PW109_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511158.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1759..1959)
                     /locus_tag="PW109_001323"
     CDS             complement(1759..1959)
                     /locus_tag="PW109_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2511159.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2264..3496)
                     /locus_tag="PW109_001324"
     CDS             complement(2264..3496)
                     /locus_tag="PW109_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2511160.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3750..4319
                     /locus_tag="PW109_001325"
     CDS             3750..4319
                     /locus_tag="PW109_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2511161.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4497..4937)
                     /locus_tag="PW109_001326"
     CDS             complement(4497..4937)
                     /locus_tag="PW109_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2511162.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5095..5859
                     /gene="map"
                     /locus_tag="PW109_001327"
     CDS             5095..5859
                     /gene="map"
                     /locus_tag="PW109_001327"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2511163.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5891..6814
                     /locus_tag="PW109_001328"
     CDS             5891..6814
                     /locus_tag="PW109_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2511164.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6890..8029
                     /locus_tag="PW109_001329"
     CDS             6890..8029
                     /locus_tag="PW109_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2511165.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8022..8822
                     /locus_tag="PW109_001330"
     CDS             8022..8822
                     /locus_tag="PW109_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2511166.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8822..9649
                     /locus_tag="PW109_001331"
     CDS             8822..9649
                     /locus_tag="PW109_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2511167.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9627..10361
                     /locus_tag="PW109_001332"
     CDS             9627..10361
                     /locus_tag="PW109_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2511168.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10461..11327
                     /gene="rfbA"
                     /locus_tag="PW109_001333"
     CDS             10461..11327
                     /gene="rfbA"
                     /locus_tag="PW109_001333"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2511169.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11341..11913
                     /gene="rfbC"
                     /locus_tag="PW109_001334"
     CDS             11341..11913
                     /gene="rfbC"
                     /locus_tag="PW109_001334"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2511170.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11935..12963
                     /gene="rfbB"
                     /locus_tag="PW109_001335"
     CDS             11935..12963
                     /gene="rfbB"
                     /locus_tag="PW109_001335"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2511171.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13061..13912
                     /gene="rfbD"
                     /locus_tag="PW109_001336"
     CDS             13061..13912
                     /gene="rfbD"
                     /locus_tag="PW109_001336"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2511172.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13947..15980
                     /locus_tag="PW109_001337"
     CDS             13947..15980
                     /locus_tag="PW109_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511173.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16024..17304
                     /locus_tag="PW109_001338"
     CDS             16024..17304
                     /locus_tag="PW109_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2511174.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17708..18514
                     /locus_tag="PW109_001339"
     CDS             17708..18514
                     /locus_tag="PW109_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511175.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18526..19746
                     /locus_tag="PW109_001340"
     CDS             18526..19746
                     /locus_tag="PW109_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511176.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19736..21322
                     /locus_tag="PW109_001341"
     CDS             19736..21322
                     /locus_tag="PW109_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2511177.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21361..23499
                     /locus_tag="PW109_001342"
     CDS             21361..23499
                     /locus_tag="PW109_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2511178.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7492 a         3944 c         4841 g         7248 t
ORIGIN      
        1 atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa agtaattaat
       61 ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc tgtaacagct
      121 gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga agttttaaac
      181 aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc tcctcaagtt
      241 aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc aggagagcat
      301 tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga tatcaaggtc
      361 aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag taccatcaaa
      421 tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg catcggtaat
      481 tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa atgataatca
      541 acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg gcaaaatttt
      601 tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat atgatgaaga
      661 tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct cagattacga
      721 tgttttggat actctcgata atgtattatc tgactataaa tatatgggga aatctgtagt
      781 agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt tccaaacaaa
      841 ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct gtttcaagag
      901 catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat gatattattt
      961 ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt tcaacagtta
     1021 aggaattgaa taccttagga atacataata gtgggtatag aatgttagag aaaagaaaat
     1081 gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg atgaatcgag
     1141 aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt gtttgttatt
     1201 tttttattta atctttgtat ctttctggat caacgaaagt atactttata tagtcataac
     1261 gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa aagtatacac
     1321 ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta gaatagtaga
     1381 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc catactttat
     1441 aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt gtttttcctt
     1501 ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt tttgtgaagg
     1561 cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat gtgccaaaaa
     1621 aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga tttcctgcaa
     1681 taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata acccctgtat
     1741 cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg
     1801 tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg
     1861 cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa
     1921 agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa
     1981 acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta
     2041 gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat
     2101 tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata
     2161 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
     2221 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
     2281 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
     2341 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
     2401 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
     2461 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
     2521 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
     2581 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
     2641 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
     2701 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
     2761 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
     2821 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
     2881 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
     2941 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
     3001 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
     3061 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
     3121 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     3181 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     3241 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     3301 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     3361 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     3421 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     3481 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     3541 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     3601 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     3661 aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
     3721 ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac
     3781 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
     3841 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
     3901 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
     3961 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg
     4021 caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag
     4081 agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg
     4141 tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg
     4201 ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga
     4261 tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa
     4321 tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt
     4381 tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg
     4441 aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa
     4501 atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca
     4561 ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca
     4621 tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca
     4681 ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca
     4741 tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg
     4801 aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca
     4861 aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg
     4921 atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat
     4981 accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct
     5041 aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt
     5101 actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat
     5161 gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta
     5221 tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg
     5281 tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa
     5341 aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc
     5401 atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg
     5461 atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg
     5521 attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt
     5581 cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat
     5641 tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct
     5701 atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca
     5761 agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca
     5821 agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg
     5881 ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat
     5941 tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc
     6001 ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt
     6061 tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt
     6121 ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt
     6181 atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat
     6241 tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt
     6301 ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg
     6361 gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat
     6421 cgatcagttc caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt
     6481 cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac
     6541 gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta
     6601 ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg
     6661 gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga
     6721 atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa
     6781 attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta
     6841 tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc
     6901 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
     6961 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
     7021 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
     7081 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
     7141 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
     7201 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
     7261 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
     7321 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
     7381 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
     7441 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
     7501 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
     7561 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
     7621 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
     7681 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
     7741 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
     7801 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt
     7861 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
     7921 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
     7981 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
     8041 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
     8101 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
     8161 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
     8221 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
     8281 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
     8341 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
     8401 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
     8461 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
     8521 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
     8581 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
     8641 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
     8701 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
     8761 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
     8821 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
     8881 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
     8941 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
     9001 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
     9061 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
     9121 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
     9181 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
     9241 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
     9301 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
     9361 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
     9421 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
     9481 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
     9541 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
     9601 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
     9661 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
     9721 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
     9781 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
     9841 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
     9901 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
     9961 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
    10021 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
    10081 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
    10141 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
    10201 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
    10261 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
    10321 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
    10381 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
    10441 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
    10501 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
    10561 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
    10621 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
    10681 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
    10741 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
    10801 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
    10861 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
    10921 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
    10981 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
    11041 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
    11101 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
    11161 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
    11221 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
    11281 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
    11341 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
    11401 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
    11461 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
    11521 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
    11581 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
    11641 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
    11701 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
    11761 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
    11821 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
    11881 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
    11941 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
    12001 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
    12061 gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
    12121 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
    12181 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
    12241 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
    12301 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
    12361 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
    12421 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
    12481 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
    12541 gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
    12601 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
    12661 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
    12721 ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca
    12781 gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag
    12841 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
    12901 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
    12961 taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
    13021 gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg
    13081 aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt
    13141 ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac
    13201 agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga
    13261 agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact
    13321 tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg
    13381 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
    13441 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
    13501 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
    13561 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
    13621 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
    13681 tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga
    13741 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
    13801 gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg
    13861 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
    13921 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    13981 aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    14041 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    14101 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    14161 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    14221 tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa
    14281 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    14341 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    14401 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag
    14461 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    14521 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt
    14581 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    14641 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    14701 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    14761 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    14821 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    14881 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    14941 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    15001 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    15061 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    15121 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    15181 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    15241 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    15301 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    15361 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    15421 tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    15481 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    15541 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    15601 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    15661 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    15721 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    15781 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    15841 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    15901 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    15961 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
    16021 agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    16081 ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca
    16141 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    16201 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    16261 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    16321 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    16381 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
    16441 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    16501 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
    16561 agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc
    16621 gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    16681 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    16741 attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    16801 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    16861 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac
    16921 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    16981 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    17041 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    17101 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    17161 gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat ggaagaggca
    17221 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    17281 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    17341 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    17401 aaaatctggc tctttgtcaa taaggactga tgggttgtac aaaatcaaat ctgggtcaga
    17461 atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt attgacaaag
    17521 agccttttcg gctctataat ttatgggact gaagaatcaa ttctgattca tcagtcccat
    17581 aaattataga gccgaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct
    17641 cggtaccaaa aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg
    17701 aacacaaatg tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt
    17761 actgctacag ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat
    17821 cttatgggcg tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt
    17881 tggactgaaa gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg
    17941 gatgattccc tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga
    18001 atatacttat ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat
    18061 cctatccaat ctgtacaccc atttattctt cattctgatt ggcatagctg tcgcgcttat
    18121 aaatggtata tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg
    18181 tgctttcttg acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat
    18241 catgcaatta attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag
    18301 ccctaatcag tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt
    18361 tgttcaaggt tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata
    18421 tactttgtat ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg
    18481 tagattgaaa ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca
    18541 attagactaa aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt
    18601 cgtgaagccc tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac
    18661 gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga
    18721 aaatctacaa tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa
    18781 atcgaaggaa aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct
    18841 ggctatgaaa atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat
    18901 gaaatggtcg atgatatcat tgaatttgct gacatcggcg accatattta tcaacctgta
    18961 aaaacctatt caagtgggat gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat
    19021 cctgatattt tgattgtgga tgaagcattg gcagtaggag atcttgaatt tcaactaaaa
    19081 tgtatggaaa aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat
    19141 gatattagtt cgattcgccg tttttgtgat cgttcctatt ggttgaaaaa tggaaaagtt
    19201 gtcgaatatg gtgatacgat ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa
    19261 tcagttaaaa cagtagatcg aaataaaaac caagtagaac gcttcgcagc tccagatatc
    19321 gtagacgtga cgaaagccga attgttggat aaagataaaa atccagtgga tatgctggaa
    19381 caaggagaaa aactatacgt taaagtaacc tatgaagtaa aagacgatac aatcaaaaat
    19441 ccagttttag gaattgctct gcgtacagtc gacaattttt atgtgtgcgg actcaatact
    19501 ctactagatg gtatcaagat cccttggaaa aaaggtgaga atatctttta tctggagtat
    19561 gaaaagttag gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat
    19621 gcaacggttc ctttggtata caagacaaaa tacatgaact tgtttgtcag cggttcttat
    19681 atcggagaag gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa
    19741 atatgatttt gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat
    19801 caaaccagga agtaaagttt tagagtttgg tcctggaaac ggccgattaa cgaaacattt
    19861 gatcggagca aaacaatgtg aagtgagcat tgtcgagcta gataaggaat tgttcgattt
    19921 tgtcagcgag tttgcccaag atggatttta tggagatatc gaaagttttg aatgggcaaa
    19981 ctattatgct ggacaaacat ttgattacgt tttgtttgcc gatgttttag aacatttggt
    20041 agatcctgga aaaacgttga agaaagttag agaatttttg aatgaagaag gagaaattct
    20101 aattacattc cctaaccttg cgcataattc tgtcatgatt gatttgttca ataatcaatt
    20161 gccatgggct tcttatggtt tgctagatga aacccataat tctttttata ctcatgatgg
    20221 cttccaaaaa gtttttgaaa aagcaggatt atacatcaat attgaagatt atttgtactt
    20281 agctgtaggt gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgtta
    20341 tgatttcaaa atgcgaccat ttggcgaagt ctaccaatac ttcttctcat tgatgaaaca
    20401 tccagtagca caaagttcca ttgcgcagcc gcaaaattct aattatgtga aagtattaga
    20461 agtaatccag caaacgaaac aagatgaaat aattcaacaa ataccattca ataatttcac
    20521 aggggaaaat gagacactaa gtttccatgt atcagaatta gcagaacgaa tggtgtttcg
    20581 cttcgcgcag caacctagct ttatcgaatt cagcgcagaa gctgctggag aaaaactcac
    20641 atttatcgat tcaaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatggtaa
    20701 agatttacca gagttctttt taacggatat tgcagggaaa gaagtgacta tccattgtca
    20761 ctatcgtttt atcggagaat tgacaccaac aatgaaagaa ttgctggaaa caatccgtcc
    20821 aatggctgaa gttacaaagc agctttctga gaaaaatgat gaattagaaa gagaaaatca
    20881 ttatcttatt gagaataata caagattaga tcagacgttg aaaacaacca taaatcgcta
    20941 ttgcacgttg ttggaaagtg atgaatggac aatcaaacat agattagggc gacgtaagaa
    21001 agaaacatcg aaaaaaatcc aagaaaaaga gctttctcta tgtattgacg aaaaaatctg
    21061 ggatgcagaa acaaagatat tgaaaatcat cgggtgggga atcgctaatt cggatcgcca
    21121 accattgtcc tataagttat cagccgacca atcaccattt tttgaagcaa ttcctgtttt
    21181 acgagaagaa gtcaatcaag caaaacagct agctgaagga gcaaaagcag gatttgaact
    21241 gcggatcctt tgcgagaagg aacgttcatt cttagttgaa gcagtggctc aaaatggtca
    21301 atcatggatc attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc
    21361 gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc
    21421 gatttgctta ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag
    21481 gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat
    21541 ttatataatc ttgatgttaa gacccaaagc ggttttaagg tacgtttatc agggaaaatg
    21601 agaggaaaag caatcttaga cttccaaaca gcaagacatc aaaatggcat agctgtgaaa
    21661 ttaaacggta aatatcctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga
    21721 ttactgaaaa aagggataaa ttatgcaagg actcatggcg taaaaaaagc aatccgccgg
    21781 gtcaaactgg aattgaaacc aggatcgatc gactatgcag agtggatcag tcgtcatgag
    21841 aaacctgatc tgaaagagca aaggaaagaa gtacaaggat ttgcctaccg cccattgatt
    21901 tccatactta tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg
    21961 gtacttgatc aaacgtatga tcactgggaa ctatgtatca gtgatgatgc ttctactgat
    22021 ccagctatcc gaaagtgtct agaatcctat caagcaaaag atgatagaat taaagtcgtt
    22081 ttccgacaag aaaatggaca catcagtctg gcaactaatt ctgcattgga aatggcagaa
    22141 ggagagttta tagcgctctt agataatgat gacgaattac ctccatttgc cttatatgaa
    22201 gtggcaaaag tgctaaacgt acatccagaa ctggatttga tctacagtga cgaagataaa
    22261 atcgatgcag atgggaatcg ctttgacccg cacttcaaag cagactggtc tcccgataca
    22321 ttgatgggaa ataattatat ctctcactta ggcgtatatc ggacaagcat tgttaaggaa
    22381 cttggcggtt tccgtaaagg atatgaaggc tctcaagatt atgatttggt tcttcgtgtg
    22441 acagaacaaa ttcctgcaga acacatttac cacattgacc gtgtcttgta tcattggcgg
    22501 acgatcccgg gatcaactgc aagcaatgga gaagctaaaa gctacatcta tgattctgga
    22561 gtgaaagcat tgacagatgc tctatctaga agaaatatca agggaagcgt acatcctggc
    22621 agaatttctg gtttttatga aatcacttat gatgttttgc aagaagactt ggtcagtgtg
    22681 atcattccaa ctaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa
    22741 aaaacgtctt atccaaatta tgaaatcatt attgcagata atggaagtac agatcctaaa
    22801 atgcaagaat tatttgcaga atacaagcat cagctgaaag accgatttat cgtcgaatta
    22861 attgacattc cattcaatta ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc
    22921 aaatactttt tattcttgaa taacgataca gaagtgattg aacctgactg gatgacagcg
    22981 atggtttctt atgcacagtt tgatcgaatc ggctgtgtag gagcaaaatt attttatccg
    23041 gatgatacaa cgcagcatgc aggtgtatta ttagggatcg gtggcgttgc aggacatgca
    23101 ttgaataact atgatcgaac acattgtggt tactttggcc gattggtcat tgacgtcaat
    23161 tacttagcag tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat
    23221 ggatttgatg aaatattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat
    23281 gaactgggtc gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa
    23341 tcaagaggtt atgaagatac accagaaaaa caaagacgtt ttgctgggga aatcaaaaaa
    23401 atgcaagata agtggccttc ttatattgct catgacccat tttataacga taatctaact
    23461 aaagaaggaa tcggcgattt ttcgttgaga cctgattgat taaaagaact gtattttcat
    23521 tttta
//
LOCUS       DANKHK010000037        23293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000037 DANKHK010000000
VERSION     DANKHK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(46..612)
                     /locus_tag="PW109_001343"
     CDS             complement(46..612)
                     /locus_tag="PW109_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2511179.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(640..2142)
                     /gene="eat(A)"
                     /locus_tag="PW109_001344"
     CDS             complement(640..2142)
                     /gene="eat(A)"
                     /locus_tag="PW109_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2511180.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWELGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFVYFPQQTKDKERLTYYVLNDITDFEIWEIERELQF
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(2400..3521)
                     /locus_tag="PW109_001345"
     CDS             complement(2400..3521)
                     /locus_tag="PW109_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2511181.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3646..4587
                     /locus_tag="PW109_001346"
     CDS             3646..4587
                     /locus_tag="PW109_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2511182.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4737..5633)
                     /locus_tag="PW109_001347"
     CDS             complement(4737..5633)
                     /locus_tag="PW109_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2511183.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGMKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5902..7242
                     /locus_tag="PW109_001348"
     CDS             5902..7242
                     /locus_tag="PW109_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2511184.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIILALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7460..7903
                     /locus_tag="PW109_001349"
     CDS             7460..7903
                     /locus_tag="PW109_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2511185.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8168..9586)
                     /gene="nhaC"
                     /locus_tag="PW109_001350"
     CDS             complement(8168..9586)
                     /gene="nhaC"
                     /locus_tag="PW109_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2511186.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(9989..11212)
                     /locus_tag="PW109_001351"
     CDS             complement(9989..11212)
                     /locus_tag="PW109_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2511187.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11243..12082)
                     /locus_tag="PW109_001352"
     CDS             complement(11243..12082)
                     /locus_tag="PW109_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2511188.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12099..12779)
                     /locus_tag="PW109_001353"
     CDS             complement(12099..12779)
                     /locus_tag="PW109_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511189.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(12781..13854)
                     /locus_tag="PW109_001354"
     CDS             complement(12781..13854)
                     /locus_tag="PW109_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511190.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETQLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(13910..14148)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14191..14424)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14501..15172)
                     /locus_tag="PW109_001355"
     CDS             complement(14501..15172)
                     /locus_tag="PW109_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2511191.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERNM
                     KLGIATTTVTKRATDILDHFKIADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KQTEK"
     gene            complement(15259..17937)
                     /gene="mgtA"
                     /locus_tag="PW109_001356"
     CDS             complement(15259..17937)
                     /gene="mgtA"
                     /locus_tag="PW109_001356"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2511192.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPTVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKET
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            18596..19033
                     /gene="ndk"
                     /locus_tag="PW109_001357"
     CDS             18596..19033
                     /gene="ndk"
                     /locus_tag="PW109_001357"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2511193.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19055..19921)
                     /locus_tag="PW109_001358"
     CDS             complement(19055..19921)
                     /locus_tag="PW109_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2511194.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20081..22174)
                     /locus_tag="PW109_001359"
     CDS             complement(20081..22174)
                     /locus_tag="PW109_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2511195.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYARTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            complement(22650..23273)
                     /locus_tag="PW109_001360"
     CDS             complement(22650..23273)
                     /locus_tag="PW109_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511196.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         6683 a         4828 c         4091 g         7691 t
ORIGIN      
        1 aagcagtgcc ttgaaatatt ttatttaaat aacacaaaca cataattact cataagaaac
       61 caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata
      121 agcattgatc actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc
      181 ttcaatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc
      241 atttgggaat cgtacagcga atgctaaacg tgctttttga aagttctcat ctttgcttgg
      301 cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa
      361 gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat
      421 gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat
      481 ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca
      541 agttgtttct ccgtgtctcg ttacataaag catgtgattt tctccttcgt tggcaaagtt
      601 ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattttct caagagaaat
      661 aatctcagta gatactttat ccaggaaggt ttgatcatgt tccaccagta acatggcagg
      721 tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa
      781 tggttcatcc catatatata gttcagctgg ctgtgataaa gatttagcca attccacttt
      841 tttccgttgg cccatactca tctgctcaat cttgttatga aaaacatctc tttccatccc
      901 aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc
      961 cgccaacgtt cctcgattgt cttcataatt ttggcgtaca taactaatgc tcagatgttt
     1021 tggctgggat ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat
     1081 ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt
     1141 aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa
     1201 aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc
     1261 ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga
     1321 tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc
     1381 agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc
     1441 ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg
     1501 agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg
     1561 ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa
     1621 tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac
     1681 ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt
     1741 gtcatccaca aataaaagtg ccaataggac ttttgtcttc tctccccccg atagtgtgct
     1801 gaattctctc cataagattt caggatctgt ttgcataaat tggagctctc tttcgatttc
     1861 ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt
     1921 tgtctgttgc gggaaataga caaattcttg ctgatggatt acttgccctt gataaggtag
     1981 tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc
     2041 tagttcccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct
     2101 gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa
     2161 aaaagaccag tcctattttc agaactggct aaagaggatg caagggatgg gtacacacaa
     2221 aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gctcccttgt
     2281 ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg
     2341 atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac
     2401 tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct
     2461 aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag
     2521 acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg
     2581 atgattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg
     2641 atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt
     2701 ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac
     2761 aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc
     2821 acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct
     2881 agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct
     2941 gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt
     3001 cttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct
     3061 aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct
     3121 aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttgataag ttctcctaag
     3181 gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc
     3241 aatgcttgtg cagattcgat gactgcggta ttttttcctt gccaagttgt caataaatag
     3301 ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccccg
     3361 agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt
     3421 tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa
     3481 gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt
     3541 cttaagtaag acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt
     3601 ttctatatac taaaagggaa ctcattgata aaggagagaa aaaacatgaa aatcgcaata
     3661 gctggagccg gagcaatggg cagccgaatc ggattgatgc tacatcaaag cggaaatgag
     3721 gtcctcttga tcgatcgttg gcctgcacac attgaagcca ttcgtacaaa tgggttgatt
     3781 gctgacttta acggtaaaga agtggtcgca aaactgccaa tctattcacc agaagaaatc
     3841 atcgaaagca acgaacacgt ggacttgatc gtagcattaa caaaagcgaa tcaattagat
     3901 gatatgtttc gttcgatcca atccattatt actgacaata cttatgtttt gtgtttacta
     3961 aatggattag gtcatgaaga tgtactggaa aaatatgtgc caaagaaaaa tatcttattt
     4021 gggatcacga tgtggacagc tggtctagct ggtcccggaa aagtcacatt gttaggtgat
     4081 ggagaaatcg aactagagaa cttggaacca gaaggcgaag catttacaaa aaaagttgtg
     4141 gaagtctttc aagaagcaaa attaaatccg atctatagcc ataatgttcg ttattccatt
     4201 tggcgaaaag cttgtgtaaa tggaacattg aatggtttat gcacgatttt ggattgtaat
     4261 attgctgaac taggcgcgca aaaaacggca gagagcatgg ttcgaacaat cgtatctgaa
     4321 tttgcttcta tcgcagcaaa agaaggaatc atattagatc aagaagaagt gtaccaacat
     4381 attgcttcta cttatgatcc tgacaacatc gggttgcatt acccatctat gtaccaagac
     4441 ctgatcaaaa accatcgttt gactgaaatc gactacatca atggtgccgt ttggcgaaaa
     4501 ggacaaaaat atgatattgc tacaccatat tgtgcctttt tgacccaatt agtccatgca
     4561 aaagaaggaa tccttggcgc agaataaaag gctctctgat gagaatcagc tgaatcagca
     4621 gctggttctc tttttttaag cccaatgaaa gatcaaaaag aaaacacaac tctcatgatg
     4681 ccataagaat tgtgcttgtt tgtttcggta cataatctct tcaaaatgta ggttttttat
     4741 ttattttcaa cagaggagag gtcatattcg tattgctcat aaatagtgga atcgttagtg
     4801 ataaatcgtt ttgcccaatt ttctgcatca taaaattcca atagctctga tgggattggc
     4861 ttaaaaggtg tatgcagaaa ttccggtaat tcatctaaat cacgttctcg aagcaaaaaa
     4921 atattttcag gacgataaaa atgataatga gcaatcgctt gattatccgt cactagtttc
     4981 ttttgaaaat acatagattc aaatgtccgc aaagtcactc cttgctgacc ctcccgcaac
     5041 agttccagaa tcccgcgcga ttcccctgtt tttttcaggt actcttcata agcaagatat
     5101 ccaggttgtt cattccctcg atgtggaaga atgaaatagt tggtcgtaac atctcttttt
     5161 ataaattcct gcttatagct ttctgctcgt tcttttctcc cattattcgt tgcaccaaaa
     5221 aatagatccg tctttggttc gctagaagaa aaatccatat gtttcgaata aaatgaacta
     5281 ttatgtttca tcccaagttc ttttgcttcc aaaaaatcaa aatgataaat ctcatcgatt
     5341 gccggttcat cttgtgcctg cttcagcatc gcctcgtgtt ctgctttgaa gtgattccaa
     5401 aaaaataaca caatttttgc attggtaaat gggcgcacgc gtcgagccat ctccatatta
     5461 aatccataat ctaaaaaaac gatcagctga taatttgcca actgttgtct agctgattta
     5521 atgaattccg actgtagtaa atcttttccg gaccaaacat ctaaagagcg gatggaaggc
     5581 aaaaaataaa gtgatttatt ttttgcaact agcagtacgt catttttgtt catcttttcc
     5641 gcctcacttt ctctgtcact ttttcctatg atagcctgtt taaatagtaa aaagccagta
     5701 aaacgcttcc tatatttgta tgaaaattta acatttgtat gaaaatatga tgaaataaat
     5761 cattcttcta ttaaaattcg cctaaaagtt ttctgaatag ttgtaattgt ctgaaaattt
     5821 gttatcatta gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa
     5881 tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga
     5941 catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc
     6001 gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg cgggctgatt
     6061 ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac
     6121 ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg
     6181 gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt
     6241 ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta
     6301 actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct
     6361 tttgctaaaa tcagtgtcat tatcttacta atcatttttg ctacttatct tgtcggtcag
     6421 caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt
     6481 actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc
     6541 ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct
     6601 aaagccatca aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg
     6661 cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg
     6721 gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt tattatcctt
     6781 gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt
     6841 ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg
     6901 caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt
     6961 gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc
     7021 tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat
     7081 aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc
     7141 acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt
     7201 tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta
     7261 ttttatttat gtggaataga tggaactatt gaagaaagga gttgtgacaa aagtcttgtt
     7321 gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc
     7381 ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa
     7441 aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc
     7501 aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt
     7561 cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct
     7621 gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag
     7681 tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac
     7741 agcttggatg aaggccatga ttcctcatca ttctatcgct atcctgacaa gtaaacgagc
     7801 tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcatccagg cacaagaaga
     7861 agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat
     7921 aaaaaaagag gatgttccag aagtgatcag cttcaagaaa taagaagaag ctacttctgg
     7981 aacaccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     8041 acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     8101 tgaacaccgt ttattcgtaa aagggattgt gacaaaaaaa gtcttgtcac aatccctttt
     8161 tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt
     8221 tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac
     8281 tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac
     8341 ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc
     8401 tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag
     8461 gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca
     8521 atgttgtaaa aatcaatttt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg
     8581 ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg
     8641 acacagtagg catcatactt tcgattcctc cgcgactaag cagtgaatcg atttgttggt
     8701 ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt
     8761 gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa atcgtcaaaa
     8821 ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga
     8881 ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg
     8941 cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggaa
     9001 cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg
     9061 ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa
     9121 tagctccaac aaccagcgct ggatttaggc ctagtgttgt cccaataccg aaaaatgcaa
     9181 tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca
     9241 caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta
     9301 gagttggaat gatccctgct tgtatccata cagcaatcaa tgaacctact aaaataaaaa
     9361 taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg
     9421 tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga
     9481 tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta
     9541 atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt
     9601 ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat
     9661 ccgctattcc tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc
     9721 gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt
     9781 atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt
     9841 ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtatgaag tttaccttcc
     9901 ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta
     9961 taactttcac acacaagttt attttttact agaaagtagc accctcttgt ttttttcttg
    10021 ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat
    10081 caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac
    10141 ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta
    10201 ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac
    10261 ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga
    10321 agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc
    10381 caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt
    10441 catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt
    10501 aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat
    10561 cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca
    10621 aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga
    10681 tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga
    10741 cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg
    10801 atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt
    10861 catcaataaa ttgctccact atgctggcgg ctaatacgga gtaagtacca aacaaattat
    10921 gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg
    10981 gtccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt
    11041 tttctaccca aaacccttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact
    11101 catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta
    11161 aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt
    11221 cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg
    11281 atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta
    11341 ttttttgata cgttttattt ttttcatctt cttttcttgc aacgatgata ttcacataag
    11401 gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag
    11461 catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg
    11521 tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa
    11581 cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca
    11641 gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga
    11701 tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc
    11761 cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt
    11821 ggaaagaatt caaatcaatc gatccatctg ctaaagccgc atttggttga ttgtaatctg
    11881 taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc
    11941 atacatctgt atcttctcca ataattccta gttttacggt agtttctttt ttgtcagttg
    12001 tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta
    12061 caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc
    12121 agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca
    12181 atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg
    12241 cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc
    12301 gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat aataccgatt
    12361 ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg
    12421 ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct
    12481 ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag
    12541 ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga
    12601 cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattccg
    12661 atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg
    12721 acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt
    12781 ttatcttcct ccttcgatca ctttgatacg actatctaat tcgttttttt gtaaaatatt
    12841 tatttttaca ccagatttga tcaaataatc caatgctttt tgccgctgtt tggtttctcc
    12901 agctaaagta acgatcaaac tccctaactg cgtttcttgt aataactcta cattgccata
    12961 taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt
    13021 ctgatttcca atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc
    13081 agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc
    13141 tttggtcaat tcttcttcag gattgctgaa gatctggatg ctactacctt tttcaatgat
    13201 tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg
    13261 agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat
    13321 ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg
    13381 ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt
    13441 aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg
    13501 acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat
    13561 agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt
    13621 tcgcaattct ttgggagaga gctgactcaa attcgtctga ttgacaatga cttctcctgc
    13681 tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta
    13741 accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg
    13801 gatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat
    13861 tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc
    13921 ttttttgcgt gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta
    13981 cgatcatact ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga
    14041 tgaattgctc gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc
    14101 tctctgtcaa aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa
    14161 aagtcctttt actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt
    14221 ttttcggaag tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt
    14281 taacggtcga acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt
    14341 tcagcttttc ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact
    14401 tactttcctt ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac
    14461 taaaaagtca agcacgaaat tcaaattatt ttttctatat ttatttttct gtttgtttat
    14521 ctaacatttc aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct
    14581 tttcttcgac ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc
    14641 ctgctcgtga agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag
    14701 cttctaatcc tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctctg
    14761 acacttcgtc tccgaatgta aacgtgtcaa agtaatcagc tattttaaag tgatccaaga
    14821 tatctgttgc tcttttcgta acagtggtcg tagcaatgcc tagtttcata ttacgttctt
    14881 ttaagtctgc caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct
    14941 gttgataaaa atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg
    15001 ctgctttcac taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga
    15061 tttcttttgg gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg
    15121 ttttttctgt gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat
    15181 taatcctttc caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca
    15241 aagaaccagc cttctcgttt ataagagttc ttggaatcga tggatataga ttttcttcac
    15301 agctgtcaca agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc
    15361 aggaagtggc atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac
    15421 aataattcct aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg
    15481 tactttcggt gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa
    15541 ccatcctgca tggaataatg cgatgaaaat cacttgctgt gaactatcca atgtatggaa
    15601 acttccgcca actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc
    15661 aaatatcgaa cttgttgggc caaagtaaac catgaatttc ccaatactag atgcattcca
    15721 tttttttggc tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga
    15781 aacatcatag attagattca aaaacagaag ttgtaaaggc agcattggta aaaatggcag
    15841 gaacatactt gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt
    15901 gatatatttc atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat
    15961 cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt
    16021 atctacagaa ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc
    16081 taaaaatccg acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg
    16141 aggattcaat tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact
    16201 catttccgag attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca
    16261 cactgaacgt gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc
    16321 tttcagagct ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagtaagata
    16381 accaatcaaa accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt
    16441 ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc
    16501 aagtacggct tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa
    16561 tttagaaatg ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc
    16621 tggatcagca accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac
    16681 tttttgataa ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac
    16741 atctaacaaa tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac
    16801 acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt
    16861 caatgtcccc gttttatccg tgcataacac atcgattgca ccaaagtttt ggatggcatt
    16921 caaatttttg atcactgttt cttttttggc catcacactt gctcctttga ccaaattcgt
    16981 cgtaacgatc attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag
    17041 aaaagcttct agccaatccc cttttgtaag cccattgatg acgataactg ttggagccat
    17101 caatgacata aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga
    17161 ttgaattggt ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac
    17221 agaaacaatg attccttccg cacttccact tatgacatta cttcccataa agacaagatt
    17281 ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg
    17341 atagctttcc cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg
    17401 catatccgct ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc
    17461 ttccatagga atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag
    17521 tgatttcagt ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc
    17581 gctgacgatg accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc
    17641 tgctaataca tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa
    17701 tggcgtgatg tatgctttca atatttctac gatgaatggt gtttttttgc catatgaaat
    17761 cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc
    17821 tgatgcatgg aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat
    17881 tttttgtgta tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg
    17941 ttctcgctgc ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg
    18001 ctgagaacag atagataata gccttggcct ttcctctaga tccttgtttt tcgaatgact
    18061 gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt
    18121 tttcgaatag ttattttccc aattagacca cctccttttt atttttcacc gatggggatt
    18181 tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa
    18241 aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac
    18301 ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg
    18361 tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt
    18421 attcggtttt ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat
    18481 atttttcata ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg
    18541 tctcattttc cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga
    18601 aagaacactt gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat
    18661 tcaacgtttt gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc
    18721 tgaattagca aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat
    18781 cgattacatg acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga
    18841 tatcgtccgt aagatggttg gggcaacaaa agctgccgat gctgttccag gtacgatccg
    18901 aggagactac gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc
    18961 tgcggtaaaa gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa
    19021 agcggcgaag tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca
    19081 gcagtttttt ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga
    19141 agaattcttg gtagtctttc gtcttttttt cccctaaagc agcagaaatc aatctttgat
    19201 aataatctgc catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc
    19261 gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg
    19321 aagtcaggtt cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc
    19381 gtcgcattct ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc
    19441 aaggaaaatc agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt
    19501 attcttcaat gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg
    19561 ttttttcgac catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat
    19621 gcctacggtt attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta
    19681 gcattcgtct ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat
    19741 attctgcaag tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt
    19801 ttttcactga atttgtctca atcccgacta gagaagtctt agaacagatt ccttccgcta
    19861 attgggactg tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca
    19921 taatagttct cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag
    19981 gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc
    20041 attagaatgt tgtctaacta ctgaaggttg gtttgattgt ttattgatta tctttttgct
    20101 gttcaacgtc tgttcttgtt actggatata agttggttgt tgttttattg ccattttttg
    20161 aaaagacact tgtcgaatgt tcgccttttt gcttttcgat ggctatgtta cgttctgctt
    20221 catttttata gtcatatttt ttcggatcga tcgtttcaaa tccgtttggc acataaaaac
    20281 gcaagagatt ttcttgattc agcttatctg aaagacttaa agcagacttg actttttcct
    20341 gatctttgga aatttcctcg tcgattcctg ctgctttcgt atcgatgatt tctcctgttt
    20401 ggttattgta agcttttcct cctaagatgg tgtatttggg tgtcacgaag ttcccatttc
    20461 gaaaagccac tacttgatca tgctgaggag ataataaatc cgtaccaaaa tggagatagt
    20521 ctttatcatc gattcccaag agatgaagta atgtcggcaa aacatcgatt tctccaccgt
    20581 attcgtgatt gactgaccct ttagtataac caggtatatg aatcattaat ggcacacgct
    20641 gcatttgtgc gttgtcaaat tcattccatg tatctgggtc ttttccaaga gcacttgcta
    20701 agtctttatt atctgtattt gagatgccaa aatgatcccc ataaatcaca aagatcgaat
    20761 tttggtaaat agcggatttt ttcaggtacg taaaaaattg ttccaatgac tgatccaaat
    20821 aatgtgccgt tcgagcatag ttatctactg tgctatttcc agtgttcaaa gatggaaaat
    20881 cgaaattctt atcatccgta taataaggcg tgtgattggt tacggaaagg aatttcgtgt
    20941 agaacggctg ttgtagatgt tcaagatatt gggcagattc actgaataaa tctttatcta
    21001 atattccata acctagcgtt tcatctgatt catcaaaata agatcgatca aagaaattgt
    21061 cataacctaa gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga
    21121 aaacagcgct tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat
    21181 tatctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca
    21241 acatattttc agcatcactt gtctttccct gtcctacttc atgaaaaaaa ttatcaaagc
    21301 tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta
    21361 ccttcatatt aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc
    21421 ctttagcgat cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta
    21481 aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt
    21541 tgattccatc ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt
    21601 caaatgttcg ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa
    21661 aaataagccc tgaaagacag gtcacagcga tagccatcgg tttgctgaca ggttgacttt
    21721 tgataggtat tttcttgatc gtcaaccaaa ccagtatccc aatgaacgta gcaatatcca
    21781 gccagtagag gacatcatgg ggtttcatca aagaaaagga gcttcctgat aacccttgcg
    21841 acactttcga aaatcccaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa
    21901 aaataatgtt caagtacaac aaaagcgtgt ttaaaatatc aatgatcaaa agaatggcta
    21961 atgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag gtagttgcaa
    22021 tcgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaac gaaaaatctg
    22081 catagtaagc aataatcgtc ttcatccaga aaaagaaaac agctgtcaaa acgattgcta
    22141 aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt
    22201 tttccttatt ttgctcctga aaaatgaaaa ttccctaaaa aagttgttat cttaagattt
    22261 ttttattctt tctaaagatt attccgtgca cttttcacag cataaaaccg ctgtgatata
    22321 agcgattgaa acagtaaggt gataaaagtc ttgtcgcagc tcctgtttag ttgatgcagg
    22381 acagcttttt cacaacttct tctttaaacg gtgttctcct gccaactcct cggccacaag
    22441 tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt ggctcagagt
    22501 taaacggcaa gctcaagacc tcttgataca cggtgttcaa tcagctgacc ttcggtatta
    22561 gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt
    22621 caaaacgctg atttcacaac ctctgttttt cattgtccat aggattgata ttgtccttgt
    22681 gatgactcat tcgtaataat tgaggcttga ttttcttcac ttcccgttat cgtttgtcca
    22741 ctgccagaat ctgaaggact tgttcctgtc gactcgtttt tttgagaaga gacagttgtt
    22801 ttcttgactt gttcatttgt ctttttccat tccgctaaac gatcggcgac cacttttttg
    22861 tctacttttt cagaataagc ggtgatcact tttccatttt tcaaacgggc tactttgtcg
    22921 ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt taaattgata ttgtctgaga
    22981 gaaatcgcac taagtacgtc atcgatatta ataaattgat tttcttcttt tccttttggc
    23041 aaatagatgt ccaattcgcc tttttcgtaa ttggcagtta ttttatactc cgcccaccaa
    23101 taagttgaga attctttttt gaaattatct actgattctt tgcttgctac gattttttct
    23161 gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga taaaataagc
    23221 agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct catgctttcc
    23281 tccactccta ttt
//
LOCUS       DANKHK010000038        22595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000038 DANKHK010000000
VERSION     DANKHK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(26..922)
                     /locus_tag="PW109_001361"
     CDS             complement(26..922)
                     /locus_tag="PW109_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2511197.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(915..1595)
                     /locus_tag="PW109_001362"
     CDS             complement(915..1595)
                     /locus_tag="PW109_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511198.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1596..2378)
                     /locus_tag="PW109_001363"
     CDS             complement(1596..2378)
                     /locus_tag="PW109_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511199.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2371..3288)
                     /locus_tag="PW109_001364"
     CDS             complement(2371..3288)
                     /locus_tag="PW109_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511200.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3452..3775)
                     /locus_tag="PW109_001365"
     CDS             complement(3452..3775)
                     /locus_tag="PW109_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511201.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            complement(4235..4627)
                     /gene="rpsI"
                     /locus_tag="PW109_001366"
     CDS             complement(4235..4627)
                     /gene="rpsI"
                     /locus_tag="PW109_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2511202.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(4643..5086)
                     /gene="rplM"
                     /locus_tag="PW109_001367"
     CDS             complement(4643..5086)
                     /gene="rplM"
                     /locus_tag="PW109_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2511203.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(5605..6072)
                     /locus_tag="PW109_001368"
     CDS             complement(5605..6072)
                     /locus_tag="PW109_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2511204.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(6192..7730)
                     /locus_tag="PW109_001369"
     CDS             complement(6192..7730)
                     /locus_tag="PW109_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2511205.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            7810..8496
                     /locus_tag="PW109_001370"
     CDS             7810..8496
                     /locus_tag="PW109_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2511206.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(8697..9224)
                     /gene="msrA"
                     /locus_tag="PW109_001371"
     CDS             complement(8697..9224)
                     /gene="msrA"
                     /locus_tag="PW109_001371"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2511207.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(9308..9985)
                     /gene="pgmB"
                     /locus_tag="PW109_001372"
     CDS             complement(9308..9985)
                     /gene="pgmB"
                     /locus_tag="PW109_001372"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2511208.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(9982..11112)
                     /locus_tag="PW109_001373"
     CDS             complement(9982..11112)
                     /locus_tag="PW109_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2511209.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(11112..13295)
                     /locus_tag="PW109_001374"
     CDS             complement(11112..13295)
                     /locus_tag="PW109_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2511210.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(13292..14317)
                     /locus_tag="PW109_001375"
     CDS             complement(13292..14317)
                     /locus_tag="PW109_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2511211.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(14362..15189)
                     /locus_tag="PW109_001376"
     CDS             complement(14362..15189)
                     /locus_tag="PW109_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2511212.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(15186..16253)
                     /locus_tag="PW109_001377"
     CDS             complement(15186..16253)
                     /locus_tag="PW109_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2511213.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(16279..17115)
                     /locus_tag="PW109_001378"
     CDS             complement(16279..17115)
                     /locus_tag="PW109_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2511214.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(17112..17981)
                     /locus_tag="PW109_001379"
     CDS             complement(17112..17981)
                     /locus_tag="PW109_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2511215.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(18051..19334)
                     /locus_tag="PW109_001380"
     CDS             complement(18051..19334)
                     /locus_tag="PW109_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2511216.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(19348..21033)
                     /locus_tag="PW109_001381"
     CDS             complement(19348..21033)
                     /locus_tag="PW109_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2511217.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            21308..22333
                     /locus_tag="PW109_001382"
     CDS             21308..22333
                     /locus_tag="PW109_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2511218.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         6333 a         4900 c         4094 g         7268 t
ORIGIN      
        1 actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag atggagaaga
       61 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
      121 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
      181 gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg gccactgtta
      241 atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
      301 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
      361 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
      421 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
      481 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
      541 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
      601 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
      661 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
      721 cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
      781 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
      841 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
      901 gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
      961 gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac tgttttctca
     1021 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
     1081 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
     1141 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
     1201 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
     1261 aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
     1321 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
     1381 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
     1441 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
     1501 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac agcttcatcg
     1561 tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga tttttggaaa
     1621 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
     1681 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
     1741 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aatgaaggcc
     1801 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
     1861 gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac aggaaatact
     1921 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
     1981 gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc
     2041 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
     2101 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
     2161 agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc gttgatgact
     2221 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
     2281 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
     2341 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
     2401 taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag gaacttttgc
     2461 ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
     2521 acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
     2581 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
     2641 cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg cgatctgtga
     2701 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
     2761 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
     2821 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
     2881 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
     2941 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
     3001 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
     3061 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
     3121 acccataata gttctcataa aggtagactt acccgctcca ttttccccaa tcaatccata
     3181 gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag caccgtattt
     3241 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
     3301 ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
     3361 agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc
     3421 ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc ttttcgacca
     3481 aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga tcaagcttga
     3541 gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc agaaaaatta
     3601 gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta
     3661 atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataaga cgtccttgta
     3721 tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa aacattaatc
     3781 cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat tagctaccaa
     3841 ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac ggtgttctcc
     3901 tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa
     3961 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg gataaataat
     4021 attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga tattatagac
     4081 caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc aaattgtgga
     4141 aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat
     4201 tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac tgtgaagctt
     4261 tacgggcttt cttaagacct ggttttttac gttcgaccat acgtgcgtca cgagtaagta
     4321 agccagcacg tttaagagct aaacggaagt caggatcaac ttctagcaat gcacgagcga
     4381 taccatgacg gatagctcct gattgtccag cgtatcctcc gccgtttacg ttaacaaata
     4441 cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg attgattact tcacgcaagt
     4501 cagcatgtgg aatgtattct tcaacatctt ttttatttac agtaattttg ccagttcctg
     4561 gtactagacg tacgcgagca actgcgtttt tacgacggcc tgtgccgata tattgaactt
     4621 gtgccaatga aattccctcc tattagatta agtttgtgat gtctaaaact tctggttgtt
     4681 gtgctgcatg tggatgttct gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc
     4741 ctaaagtgtt tttaggaagc atacctttta cagaagtttc gatcaaacga cgagaatttt
     4801 tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt
     4861 aataaatttt gtcagtcgct tttttacctg ttaatttcac tttgtctgca ttgatgacga
     4921 tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg tttattttta ccacgtagta
     4981 cagatgcaac aactgttgaa agacgtccca atggaacatc agttgcgtct actacatacc
     5041 atttacgttc tacttcgcct ggtttggcca tatatgttgt acgcacgtta ttttcctcca
     5101 atttaagttc tgatgtttga catacacttc gagtttccgg ggctctttgt ggggcaaaca
     5161 ataccattta acatattacc cgctaatcta gtcaatgtca acgtttttct gcttgattga
     5221 aaaatttata tgaaacttta cttcttttct ttcttttata taaaagggtc gtttcctgtc
     5281 aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc acaacccctt attttgaata
     5341 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccataaa acataagaag
     5401 ctgtttttta aaattgtttc ttgtgactcg gagctgaacg gcaggcttac aacctcttgg
     5461 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
     5521 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
     5581 ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc
     5641 ttggatcatc cagatacgtt tttctgtggc tgttttgaat tcgataaaga tatcttgtgt
     5701 tggatcgtcg ccttcttctc cagaaacttc gattcctttt tggtaaagat ctgctagata
     5761 acgatagcct gcaacaagtg tttccaaacg ttctggaata gtacgatccc agcggccaat
     5821 ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc
     5881 taatgtgatc aagcgttcgg atacttcatc taactgagcg ttcaaatcat ccatgaactc
     5941 atccatcatt gggtgcaatg taaggaaacc tggtcctctc atgtaccaat gtgtttggtg
     6001 aacaacaaca gacatttggc ttagatctgc aactaattga tttaatactt ctttggtttg
     6061 ttggaatttc atctttcttg cctcctaaca ttttcttaca cttaaaataa tagtagactt
     6121 tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat tttcagttcg cttttttatc
     6181 cgtaaagcca atcatttttc agaatcagct aattcttctc gcagttgagc aaattgctga
     6241 taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg gatagatttt ctgatatttt
     6301 ttatgatcag tttcttgtac tccaacttgc tctttcaatt ctatcaattc ctgtgcttca
     6361 ctgatacttt ggatctttcc gatgcttctc cagcctaaaa tcacagctcc taatgcggat
     6421 gcttctgtga cagtagggaa aacaagcggg taacccagta catcagccat catctgacga
     6481 aaaatcggag attgagcaaa cccgcctgta gcacggatct ctgtgagctg attttcctgg
     6541 caaactcctt ttaaaatccg ataaaggttg agacagatcc cttctagaat cgccttcatc
     6601 atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc
     6661 caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct
     6721 ggcacaaaag cgatagccga aagcagtgca tcatatggat tttctccttt ttctttagca
     6781 gattgaacga cttcctgtaa aaatgtatgc cctgcccaat caaagatgcc agcaccattg
     6841 ctagtcgctc ctcccactac ccaatgtttc tgatccagaa cataacaaaa cgtctccatt
     6901 tctggatgga actgaggctg agtagtaata aaacgaagtg ctccgctcgt tcctacggtc
     6961 aaagcggcga tcgtatcacc aaaagcacct aaccctagat tagaaagcgg cccgtctgcc
     7021 cctcccaaaa caaatgatat atcatttggc aaacctagtt ctgctgctgt ttttgatggt
     7081 agacctgtta cttgataggt ggaaggaaag acttgcggca attgctctat tcgaatgttc
     7141 aagtagtcaa gcacttcttc tgcccattgg aattcatgga tatcaaaata tccagttcct
     7201 gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc tataaaagat atagtctttg
     7261 ataccgacaa aataagcagt ttttttgaat aattcaggtt ggctgttttg cagccacttg
     7321 attttgttca atggtgtcat tggatgaatc ggcgtcccag tctgccgata aaattctttg
     7381 ccaagcacgg tatatttcag tttttctgct tcgtccgctg ctcggttatc tgcccaagtg
     7441 atcattcgag ataacggtgt tttctcttca tccaacaata tcaggccatg catcgcagag
     7501 gagaaggaga caaattgaat gattgtgtct gaaggcaact gtttaattac ttcttttata
     7561 acggtgcaga caccttgaaa gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct
     7621 tggatcaaag ggtagcgaaa ataagcggat gctgcttctt gggcttcatc tgtgaaagca
     7681 actgctttgg tttgtgtggt tccgatatct aatccgattg aaacgttcat tttttcacct
     7741 caaattttat gtatgtacaa aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg
     7801 ggagatggcg tgttacttta ttttatcatt ggttgctgta tcggatcatt tttatgttta
     7861 accgcccagc ggattccgtt aggtcactct attatttatc ctagatctca ttgtgtcaaa
     7921 tgctgtcgtt cgctttcttg gtatgaattg attccgattc tctcgatcat cgtccaacga
     7981 tttcgctgcc gctattgcag gtgtcggttg cccttctact atttgttggc agaagcctta
     8041 tgtggtggtc tttttgcctg gttctttact ttttcttccg caagaaattt ttcgacgttc
     8101 atatggatgc tttcggcttt gctattttcg ttgatggatt tattttactt catggtccat
     8161 tctcacacgc tattctgttc ttgggcaatt ttatggattt tttggttgca ggccggcgtc
     8221 ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt
     8281 caatcctatc tcggagctgg cgatactctg ttgatgcttt cttggagtgg tggcttgtct
     8341 ctagaagagt tgcttcagat cttgtttctt gctagtctgt ttggaatatc cgttttttct
     8401 gtttacttta tctgtcataa aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc
     8461 agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa aggttgtgac aaacgacttg
     8521 tcacaacctc tgatatatag tgttctctta ccaaatcctt gtgacttgga gctaggcgac
     8581 aggttcacaa cctctccact aaatggtgtt caatcagctg accctcagta ttagctcaaa
     8641 atgctggttt tacaacctct gatatacggt gttccataag tagctttttc ggcttcttat
     8701 ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga
     8761 ttgtaatgga gcttgttttt cttgtaataa tcctgatggt aatcttctgc aggataaaat
     8821 tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat
     8881 gcttgttttg aacgctctgc aatatttcgt tcttcttcat cattatagta gatcactgga
     8941 cgataagaat caccgcgatc agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc
     9001 caatagattt ccaccaactg atcataagaa ataacttctg gatcatattc gatctctact
     9061 gcttctgtat gccctgttgt gcctgttgtt acttgttcat aagttggatt tggtacatgc
     9121 ccgccagtat aaccggatgt cacagaaatg atccctggct gctcttcaaa tggtttgacc
     9181 atacaccaaa agcatccgcc tgcgaaaact gctttttttg tcatataaaa tccccctttt
     9241 ttctcatcat accttgattt ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg
     9301 gaaactatta tatacgtgat acggcttctg ataaatgcca aaacgaacgt acttgatcaa
     9361 aaacggttaa atcaggaatc acatattcag cagaaggaag atttatgttt tcacctattc
     9421 ccaatacatg catgttacca gccaaagctg cttgacaacc agcttctgaa tcttcaatga
     9481 ccaagcatgc atttggcggt acattcaatt gctgtgctcc tttgagaaaa acttctggat
     9541 caggttttgc ttttgatact tgcgtgccgt caataaggac atcaaaataa cttgtcaaat
     9601 tcgttttttc taaaatgagg cgtgcatttt tgcttgctga tcccaatccg atcttgatgt
     9661 ttgttttttt caagtactca agaacagcta ttgcaccagg caaaacggat gtttcatcca
     9721 ttttttgaat cgcttgaaga tatagattgt ttttttgctc agcaaaggct gatttctctt
     9781 tttctgagaa tttctgttcc atttttccaa atgacagcaa tcgctctagt gactcggttc
     9841 tggatattcc tttcagttgc tcgttttctt tctcagtgaa tggaatggac agctggttag
     9901 ctaaaaccag ccacgcttcg taatgataac gagctgtgtc tacaagaacg ccatctaaat
     9961 cgaatagtgc tgctttgatt ttcatcgatt acctccttga aaatgaacga cttgaagtaa
    10021 agatgcgatg tttttagctg tgcgatgaag attatcagtg gcttgctgta aagcttcttg
    10081 taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa atgccaacat ctgctgcttt
    10141 ttcgcttcct ttaccaacac ttccgcaaat agctatcaca ggaacgccct ctggtactct
    10201 tctagcaatc ccaactggcg cttttccttt tagactttgt ccatctagac gtccttcgcc
    10261 tacgattacg aggtctgctt gctgtacccg tttatcgaac tccagtaaat ccaatacgta
    10321 gtcgatgcca gagactattt tccctgaagc aaatgctaac agtcctgcac ccattccacc
    10381 accagcacta ctgcccgatg cctcgattac ttgaggggca aatctcatat aaaaatcatg
    10441 catggcttgg tcagccactg ctaattcatt ttctttcaag cctttttgtg gtccgaaaac
    10501 aaaagaagcg ccttcttttc cgcataaaag attagtaaca tcagtaatga cagtgatttt
    10561 ggctttaatc agctcaatag gcacgttcgt tttatcaact gtagcaattt tacctagatt
    10621 ttctcccagt gcttctactt tttgattatc ttgatcgtaa aaagcataac ctagaccttc
    10681 tgccatgccg atgccgccat cgttggtact acttccgcca acgccgatga tgatgtgtcg
    10741 aacaccttgt tgcaacaatt gtcgtatcag ttctcctact cctcgcgtgg ataacctaag
    10801 tggctgttgt tggtttagtg ggacatcttt caatccgacg atttctgcca tttcaaaaat
    10861 cgcagtttga ccgtctgaag cgtactggat ttctttgttt ttgccaaatg cattagtaac
    10921 gactgctgtt tgccgcttca accttaaact gtctgctaat gatgaaaccg ttccttctcc
    10981 tccatcgcct ataggtagca gatcatattc agcctgaaca aaaacttctt gaaaacctga
    11041 ttgaatggct tgtgctgcct ctaaggctgt cagactttct ttgaaagaat ctggcgcaat
    11101 cagaatacgc attattttcc cctccgttcc aagatcagtt ctttttccag ctggtattgg
    11161 tctccattga caaaaatagg taatgtttct ttcgtctctt tttttattac tatttgtttt
    11221 tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa aaggaaaaga aattttttcc
    11281 catgctgaag gaagtcttgg agaaatagaa agagttccat tcgttactgt aatacctgaa
    11341 aaaccaaata taactgctag ccatagtgct cctaatgaag cagcatgaat tccgtcatct
    11401 gatgaatgcg gatctggtcc taaatcaatc aggcacgctt cttggaataa atgatacgcc
    11461 cattccattt cttggatacg gcaagcctct atggcgtgaa tcgctttact caacgatgag
    11521 tcgtggattg ttttgttttc atagtaatgc aaattcttag aaacgattgg ttgagaaaaa
    11581 tactcaggta ataagtataa cagcatgaca agatctgctt gcttgctgac ttggagttca
    11641 tttacttcgc tacgggcata atcaagtaaa atagactggc tgccaggacg ttctttgtaa
    11701 gtggtcaaat caataactgg cttactgaga tacgtgtcat cttgcggaat cagctgttct
    11761 ttgttaggca ctggcaaata taacttatct aaaaaatttt gacattcttt caaaaaaagc
    11821 ggatcgccag cttgatattt aacagctaag gccacattat aatgcgccag atagttcgta
    11881 tatgcattat tgtcgatata ttctgtgtat tcatctggac caatgactct gtgtatctct
    11941 aaatgatcat cgacttttac agctctgctc agccaaaaag ctgctgtttc atgaatgagc
    12001 gtttttccct ttgtttctaa aaacgattga tcctctgttg cttggacata ctcttgaacg
    12061 gcataagcaa ggtctgcact gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt
    12121 gttccggttt tgatattcaa tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg
    12181 ctttcccatg ggaaaagtgc accttgatag tgataatctc ttgctttttg ccgtgcgcct
    12241 tctaatcgat cataacggta cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa
    12301 aagaaaggca ggataaaaat ttccgtatcc cagaaaacat gccctttata accttcgcca
    12361 gttagtccct tcgcccctac actaacactt ggatcattgc ggggagtcat aatctgtaaa
    12421 tggtaagcag cgaagtccat cgctaactga tcgaaagaag aatcgccttg gatcgttatc
    12481 cgatgctgat gccaaaaatc tgtccaataa gacatcgact gctctaaaat ttctgtataa
    12541 ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag
    12601 gtagagagca cgatgatctt ctccagcgta aaggacgtat tttttctgat cttttctgaa
    12661 aaggaagcgg tgatccggcg attttttgct tggaatacac ctgctttatt gaatttagct
    12721 gcaatcccaa tatcaatttt actttctgtt gtctggtagg atgcactgat caaatcgtca
    12781 ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca
    12841 tcgatacccg ttttcacatc tacttggcag tcttcggtca acggcttgat gaccactttc
    12901 agggctgcct gatggagatt attttgatca gcaacacgac aagtaatgac ttccactttt
    12961 tttccttgag cagtttcaat gagcgcatag cgtttaagct ctcccatttc taaatctagt
    13021 gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt
    13081 atttcccatc tgatgacatc tgggagattc actaactcgg ctgattctgc tcctaaaggt
    13141 cggttatata caccagctgc aaacattccc cttacttgat tccgatagtc ttcctcctgg
    13201 cttcctctga taccaaaaaa tccatttcca atggtcatct gactcgcata cttattgata
    13261 tactctaaat caaactgttt ttcttctact ttcataaggt aataacttct cctgtttctg
    13321 ccgaacgata taaattttcc accagattct ggatgacata tccttgccag ccatctgcaa
    13381 tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg agccatactt agttcatgtg
    13441 ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt tccatctaca tcttcgaata
    13501 gttcgcctgg atacagatta gcacctgcct gatcgccaca aaaagccaca ttcagtttgt
    13561 cttctctgat attcaaagca aacgaagtat tcagttggat gatcttgccg ttttctaatt
    13621 ccagtgtgcc aaaaattgca tcttctactg tgaatttgct tggatcccat ttcccgaatt
    13681 tccctgaatt ttttttcgta ccaatttttt ggaagctgta cgcccgaacg ctttttactt
    13741 taggaaatcc agtaatatac atcgctgtat caagcatgtg aatgccataa tcgatcaaag
    13801 gcccgcctcc ttgaagttct ttgtcgataa acgttcccca gcctggaaca ccagaccgac
    13861 gaagcgcatt cgcttctacg taataaatat ccccgagaac atcaagatga ttcttgatca
    13921 ttgttgcttc gtcagaaaaa cgatgctgga atccataagc caaaatctgc ccgtttttat
    13981 cggctgtctc tgccatcttt cttgcttgat cagctgtcat agctggtggt ttctcgcaca
    14041 tcactgaagc attagcttcg agggcagcca atacatgttc gaagtgaaag cgattgggtg
    14101 tacagacact cacgatatcg ggagaaatct cattcagcat ttgtgtaact gatgtatacg
    14161 catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg ctggctgact ggatcaacca
    14221 cagcggccaa ttctaagaca tctgatcttt tttgataata agcagcatgt actttttctg
    14281 ctacttgtcc agcgcctatg attgctactt tcttcattta tttctccctc ctttgcttat
    14341 ttacttattg ttattctttc tctattcttt ctctaaatca tttaaatgac gcgctgtttc
    14401 cagacagttt ttcaagtatt gcaatgattc ctgataaatt ttcacttcat tttctcctcg
    14461 caaacgacat tcatatacca tataaccttc atactcattt tgaagtagcg tttgaaaatg
    14521 gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga tgattatctg ctaaatggat
    14581 atgacccaca tactcttttt gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt
    14641 catatgataa aaatcagcga tgatctgcac atgtttcaat tgattagagt caatgtaatg
    14701 acgcgcatcc gctaacaaat tgatcatatg gtcttggtat cggttcaaag gctccaaata
    14761 aataaccgta cctgtttctt cggctacttt atctaaataa cgaagtgatt cgctgactgc
    14821 tttatagtct ccttctttgc ttcggggtga aaccatcggc ggtaatcgat aagtgaacat
    14881 tccccaagct gctgggataa cgattccttt gcctccaacc tcgcttagtg cacgcaaaat
    14941 ctcagcaatt tgacgtacac cattaagacg ccgttcttcg ataaagtcac cgatccaccc
    15001 atcgtagccg ttacaagctg tcactactgg gatccctgtt tctttgattg cctgtttgac
    15061 tgtgtcgata ttttctatta acaacttacc gtcgatttcg tattcttcaa atccacagtc
    15121 tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc
    15181 gaatttcatt gttctcctcc tcggacttga acgcccattt tgatactttc ttctgggtgc
    15241 tggtctacat aacgcatgta agcttctgcg ctttctgcaa atggcacaac tggatcaatg
    15301 atttcttcac aatccaagta accgttcatc aataattccc aacatgtgtc ctcgattcgt
    15361 ttacggttcc atcttgggta atctgggttt ggttcactag atgctcgagc aaagacgatt
    15421 tttgcattat tgaaatgtgc ttcgcgacca agattaaacc cagctggaaa aggtttagca
    15481 aaggcaacat aagaaatgat cccgccatag ccgatccctc ttaaagaagc ttgtaatgcg
    15541 gcagcactgc cactcgtttc aataactgaa tctactccac gtttatctgt caattccttt
    15601 agacgaaaac cgacatctgc tttcatagga tctagcgtgt aatcggcacc gtatttttct
    15661 gctaattctc tacgctgcgc tatgggatcg atcccaacga tggtagtagc acctgctttt
    15721 ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc tacagcatca
    15781 cctgctcgaa catgtgcttc ccggatacca cctaaagcga attgagcagg atcgtaacat
    15841 actgcatttt tccattgatc caaccgatca agtttacgta gtttataatt atcaacaccg
    15901 tttgtaatca gcgtttcacg aatgggtcca taagtgcaga ctaactctcc tttttgatac
    15961 tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc taattgaaat
    16021 tcgccaaata ccacaccgcg ttggcttcct tcttctcttg gcaagaatag gcgccattct
    16081 tctgaaaatt cttcatcgat aaatggactt aatcctctaa agtcgacaat ttctgttcct
    16141 ggttttggag tagcgaaaat tactcggata cgtacttcgt tcttttcgat ctcacggtct
    16201 tcgtaatctt tgaatccggc tattcttggt tctaatgcaa ctaatttttt catcttttct
    16261 ttcctctttc ttttcttttt atccttttac gccgccttcg actaaatcac ctttaataaa
    16321 tcgttcagat aacgcataca taatgatgac tggtaaagcg gtcaccaatg aagcggccat
    16381 cattcgtccc caaatgtagt ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt
    16441 aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa taatcattcc acgaaaccat
    16501 aaagcaataa acgaagacag aaatgattcc agaaatagcc aatggtataa tgatccgaat
    16561 aatgatctgg atccggctaa gaccatcgat cattgctgct tcttcaatat cagtcggaat
    16621 cgtgtcaaaa taacttttta gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt
    16681 gataatcagt gctgtattgg tatcatacaa acctagaccg gaaatgattt taaataatgg
    16741 aacaacgatc aaaataccgg aaaacatata aacggtgtaa aagctagcat tgattttcga
    16801 gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa tcactactgc
    16861 agttacggag gctatcaagg agatcactaa actatttttg aaatacatga tgaatggaaa
    16921 aatctcagca ttgaaaatat cctgataatg ttcaaaagtc cattctttcg gaaaaatagt
    16981 ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa
    17041 tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg taaaataaga ttttcctgct
    17101 ctttttattt ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac cgctgcaata
    17161 atgatgaaca atagaatcga tatagctgct gcttttccaa gatcattgaa cgcaaaggct
    17221 gtctgataca aataaacgcc taagatattg actttattcg tcagtagata gacatctgta
    17281 aacatataga acatccagat agatcgtaaa acaatcacag ttgctagtac tggtttgatt
    17341 gccggcagtg tcacgatctt gaacttctgc caagcatttg caccgtccat ttcagctgct
    17401 tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga
    17461 tagtatctcc agatagcaaa caaaacgacc ataaagaagc tagcaactgg ctggtcgaac
    17521 cacagcggtg cttcgttgaa aagctgcaac ctatctacta acagatcatt gacgattcca
    17581 taactattat tgaacatata tttccaagca aaaatcaaag aaatagatgg cgtaacataa
    17641 cttaataaaa tcagtgaacg caccgttttc ctaaatctga acttgcggtt aagcaaaata
    17701 gctacggcta atcctaaagc tgtactgcct gctacaacta agaatgtgta agcaactgtt
    17761 aaacctaaag aacgataaaa ttcactatca cttaaaattt caagataatt gcttaatccg
    17821 ataaaaacgg cgtctgagtt aggattcagc ggcatatcga aaaaactgat ctgaatattg
    17881 gaaagcattg gaatcagcac taaaacagct aataaaagca tgcttggtgc taaaagtccc
    17941 atggctaatt ttaagtcaga atgtttcctt ggacgattca ttgaaacctc cccctgataa
    18001 tctgccaata gctatctact tttttcgaac accattggca gaacttcttg ttatttcgta
    18061 tttttttgtg cgtcagctaa agcttgatcg acatcttcgt ttccgacagt tacttggttc
    18121 actgcttttc cgatcacacc tgagcttgtg atatctccca ttttcgtgaa attcttttcc
    18181 ccgactagtc caaagacttg tacttcatca aaagcactag caatctcaga tggcagctcg
    18241 ccaaaagact tgatgacttc attttctttg taaccatcca gttcgactac ttgtttattc
    18301 actggttgag caccaccagg agacatcaag acccactttt ccatgttttt cggttgagat
    18361 aagaaagctg tgaatgcttc agcagcctct ttttgtttct cgtctagacc agaagaaatc
    18421 gtcaatccag atactgtgcc atagacagct tgatcttttt gtgttgggat agcaaaaccg
    18481 atattttctg agttccccgc ttcataaaca gaaggcaaaa tgtaagttga atagattgcc
    18541 attggtgctg ttccattcat gaaagcatct ttgatttctg tcacatcatt agagcctggc
    18601 atcgtgtatt gggataagtt ttgataaaac gacaatgctt ctctcatctt ttccgtatcg
    18661 atcgttacct cgccttcatc atccaaaaca tttgctccat ttgacaaagc aaattgagag
    18721 aacgcttgct cactcattgt tccttcagca gtaggcatag caattccata ttgtttattc
    18781 tctttatctg tgaaatgttg agcaattttt aaaatatcat cccaattttc tggttctgaa
    18841 aaaccagctt ctgataattt ttctttgtca taccaaatac cttgaaccca gccgctaatt
    18901 ggtgcagcaa tatagctttt tccatcttct gaacgaacaa ggtttttggc tccgtcataa
    18961 taattatctt ctccagcttc ctcgataacg ttttggactg ccgtctgatc aatcagttca
    19021 tctttatcca tcactttagc aaaatcctga ctgacttcca ttacttctgg taatttaccg
    19081 gaacgagcta acgtaacgat ttttgtattg aacgctgatt cttctaccgg cacttgcttg
    19141 attttgatgt ctggattttc tttttcaaaa tcagcaacca gttcattgat cacacttaat
    19201 cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag
    19261 ctttgacttt caccacaagc agtaagaaga aatccagtag ctagcagaca ggtagtaaca
    19321 ataatttttt tcattttttt ccacctctta ttttattatt ttcagccaca tatattcata
    19381 cggctggaat atcaactttc gttcttgact gcaatggata atttgtccag aaaacaggtc
    19441 taggtaatct ccttttggta aaagataacc tgctgattga ttggaaaaat tatggataac
    19501 taagactttc tcttccttcg cttcgcgaat aaaactgata aaagaatctg ctgagtccag
    19561 taagtaaatc aaactattcg gatggaaggc tttttctttt tttcgaatag atagcatctg
    19621 ggtcaattca ccatatactg ctgttcttaa tggatctgtc tgcaactctt tctcaatatc
    19681 tgctaacaaa aatttttgcc gattgatttt tcgattctct cctgtttttt ctacgccttt
    19741 gtaatcattt cgacttccca gcacactttg aatataaatt gccggcacgc ctggtaacgt
    19801 taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg
    19861 agaacttaac gcatctagat acgtagcgtt tatctcatat ggactggtcg ttccatctgg
    19921 gtttttcttg tagtttactc gggtaccctc tttcttcagc tgttccacta attcaaggat
    19981 cgttttttcc ggcagaatac ctcgtaccgg attcaatccg ataccgtcat gggaggctaa
    20041 aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc attctttcaa
    20101 cttacttgta ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata
    20161 gaccatctgt gcttcattct cgccattccc aaaataactg atattatctt tgtgtggcac
    20221 atttgtctca gtgatcaata ccgttcctgg agctgcttca tcagcgattg cacggaataa
    20281 ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt ctttccataa
    20341 aaatcccaca gcgtccaagc gaataaatga agcgcctttt tctaagtaaa acaaaagaat
    20401 atcgatcatt ttcaataaaa cttctggatt tttaaaattc aagtctactt gatcttcgct
    20461 aaaagtcgtc catacgtgtt tttctttccc gtttttgtca atgaacgttg taagtaacgg
    20521 gctggtcctt ggtctagtca cgctgcttgt atctgtatcc agtggcaaat caataaaaaa
    20581 atctttgtac gtatcattca agtttaaata ttcctgaaac caagtacttt cggcagacat
    20641 atgattgcag acaaagtcga acattaaccg tgtttcgctt ctcatttctt ctatatcttc
    20701 ccatgtgccc aaacggtcgt tgattgcctg ataatttaca acggagaaac catcatcaga
    20761 tgtatatggg aaaaatggca agaaatgtac aactctaaat gtatctgcca aatacttttg
    20821 ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt gatccccata
    20881 agtaatcaag aaaatatctt cttcgtccca atgcagtttg cctgcctggc ctttcttctg
    20941 ctgataaaca ctgacttttt gttcaatcat agaggcggtt ttctcagaca aatctgagtc
    21001 gcgataaatc gcttgtaaaa gttttgtata catcggattc ccctttcttt tttgggagcg
    21061 ttcccaacga tttgatcata ttatggaaaa cgcttactgt caatagcatt tttttaaata
    21121 attttgatat acacaaaaat acaaataaga aagcttatgt atcaataatt aatagcttct
    21181 taaaaaaata tggtctctca tgatcatgca agctattcct aaattttcaa aatcatagac
    21241 aaaaaagaat gtctattgta ctatggaatt attctaaaac gctcccaaaa aggaggtgca
    21301 tataaaattg aaacctacta tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac
    21361 tgtctccaga gtattaaata accagccaaa tatctctgat aaagcaaaag caagtgtact
    21421 tcaagcaatc aaagaattga attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa
    21481 tggttttgac gcgattctag cattatctca tcgtacttct catacgacct atggcaatcc
    21541 ttttttttca gatgttattc aatccatttc tgccgtttct gaacaaaata acttcgactt
    21601 gattctacaa acggcaaaaa caccagaaga agagctagaa aaatgcgcga aaaagatcca
    21661 agaaaaaatg atccgaggaa ttattatttt aagctctacg atggatgaaa cacttctttt
    21721 tgaattggat caatacaata ttcctattgt ggtaatcgga aaagtcggaa acgactacac
    21781 gaacatctac tcagtggaca cggataattt ccaggatagt tacgatttgg tggaaacact
    21841 gatcaagaat ggacaccgtc atatcggctg tctccatgct ccgacagaag tttatgtatc
    21901 caatgatcgt ctcaatggtt accgtcaatg cttatttgat caccacctag atatccgtaa
    21961 tgaatggatt atagacggcg gctttcgctt agaagatagc atggcagcaa tgaatgattt
    22021 aatgaagcta cgagaaagac caactgcgat tttttctacc gatgcattaa aatcattgag
    22081 tatttacaaa gtggcagaac aatctggaaa gcagatacct gatgatcttt cgatcgcagg
    22141 attcagtgat cccttattgt cctccctatt ttcgccacag ttgactcgga tagacattcc
    22201 gacaaatcag ttaggaatca aagccacaga acttttattc caactaatcc accaccaata
    22261 ccctgaagaa aaaagaatca ttctttcaac cgagattact ctcacagatt cgatccaaca
    22321 acaagaaatg tagaacagaa ttataaaaga acagaaacac tgaggataag tcagttactc
    22381 tgttcttttt tcaatcactc atccctcgtt accccctccc ggtattttag cgaatttttc
    22441 ttattttcca tctgttttag atagctgtgg ataactataa tgtttgtgtt aaactatttt
    22501 ttccacaggg aagaaaaaac tatttttaac cacgttgtgt ataagttttt atagttcgtt
    22561 tttttaaaca catttaccct tatttcaatc catgt
//
LOCUS       DANKHK010000039        20909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000039 DANKHK010000000
VERSION     DANKHK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            69..437
                     /locus_tag="PW109_001383"
     CDS             69..437
                     /locus_tag="PW109_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2511219.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            607..1038
                     /gene="mraZ"
                     /locus_tag="PW109_001384"
     CDS             607..1038
                     /gene="mraZ"
                     /locus_tag="PW109_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2511220.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1059..2018
                     /gene="rsmH"
                     /locus_tag="PW109_001385"
     CDS             1059..2018
                     /gene="rsmH"
                     /locus_tag="PW109_001385"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2511221.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2023..2424
                     /gene="ftsL"
                     /locus_tag="PW109_001386"
     CDS             2023..2424
                     /gene="ftsL"
                     /locus_tag="PW109_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2511222.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2425..4617
                     /locus_tag="PW109_001387"
     CDS             2425..4617
                     /locus_tag="PW109_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2511223.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4643..5605
                     /locus_tag="PW109_001388"
     CDS             4643..5605
                     /locus_tag="PW109_001388"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2511224.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5619..6980
                     /locus_tag="PW109_001389"
     CDS             5619..6980
                     /locus_tag="PW109_001389"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2511225.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7006..8094
                     /gene="murG"
                     /locus_tag="PW109_001390"
     CDS             7006..8094
                     /gene="murG"
                     /locus_tag="PW109_001390"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2511226.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8109..9329
                     /locus_tag="PW109_001391"
     CDS             8109..9329
                     /locus_tag="PW109_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2511227.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9485..10810
                     /gene="ftsA"
                     /locus_tag="PW109_001392"
     CDS             9485..10810
                     /gene="ftsA"
                     /locus_tag="PW109_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2511228.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10833..12074
                     /gene="ftsZ"
                     /locus_tag="PW109_001393"
     CDS             10833..12074
                     /gene="ftsZ"
                     /locus_tag="PW109_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2511229.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12091..12768
                     /locus_tag="PW109_001394"
     CDS             12091..12768
                     /locus_tag="PW109_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2511230.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12782..13381
                     /locus_tag="PW109_001395"
     CDS             12782..13381
                     /locus_tag="PW109_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2511231.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13396..13683
                     /locus_tag="PW109_001396"
     CDS             13396..13683
                     /locus_tag="PW109_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2511232.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13732..14514
                     /locus_tag="PW109_001397"
     CDS             13732..14514
                     /locus_tag="PW109_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2511233.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14535..15242
                     /locus_tag="PW109_001398"
     CDS             14535..15242
                     /locus_tag="PW109_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2511234.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15504..18302
                     /gene="ileS"
                     /locus_tag="PW109_001399"
     CDS             15504..18302
                     /gene="ileS"
                     /locus_tag="PW109_001399"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2511235.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18351..19877)
                     /locus_tag="PW109_001400"
     CDS             complement(18351..19877)
                     /locus_tag="PW109_001400"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2511236.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19892..20539)
                     /locus_tag="PW109_001401"
     CDS             complement(19892..20539)
                     /locus_tag="PW109_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2511237.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20701..>20909)
                     /locus_tag="PW109_001402"
     CDS             complement(20701..>20909)
                     /locus_tag="PW109_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2511238.1"
                     /translation="QRPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRK
                     SLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         7062 a         3612 c         4462 g         5773 t
ORIGIN      
        1 aaaatgaaac agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt
       61 ttacgttcat gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg
      121 ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa
      181 aagcccctga tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata
      241 cagggacaat ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta
      301 ttgctgtttt ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga
      361 cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca
      421 tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg
      481 cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa
      541 aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg
      601 ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg
      661 ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc
      721 tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg
      781 ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa
      841 tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct
      901 ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca
      961 cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg
     1021 attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact
     1081 atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg
     1141 tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta
     1201 aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt
     1261 tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag
     1321 aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt
     1381 cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg
     1441 atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct
     1501 accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg
     1561 ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg
     1621 aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa
     1681 gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt
     1741 tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt
     1801 cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac
     1861 caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta
     1921 aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt
     1981 tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa
     2041 atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct
     2101 gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag
     2161 atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt
     2221 gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata
     2281 caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa
     2341 ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac
     2401 gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa
     2461 aagaaaaatc taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg
     2521 agtattggat tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat
     2581 gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta
     2641 aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc
     2701 tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat
     2761 gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac
     2821 aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa
     2881 tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg
     2941 aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat
     3001 ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga gaatggctta
     3061 gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag
     3121 attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa
     3181 aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca aagttattta
     3241 gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg
     3301 atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa
     3361 acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt
     3421 tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa
     3481 ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat
     3541 gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt
     3601 aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa
     3661 aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta
     3721 cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg
     3781 aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct
     3841 cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa
     3901 gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat
     3961 gttgtcgaaa gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat
     4021 gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga
     4081 gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc
     4141 ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca
     4201 aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa
     4261 acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca
     4321 gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa
     4381 aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact
     4441 ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg
     4501 gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa
     4561 cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa
     4621 gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag
     4681 ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca
     4741 aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac
     4801 aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg
     4861 gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt
     4921 aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc
     4981 gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc
     5041 tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta
     5101 tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg
     5161 catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc
     5221 ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag gaggtttact
     5281 gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt
     5341 agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct
     5401 tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt
     5461 taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg
     5521 cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt
     5581 gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg
     5641 aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat
     5701 tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg
     5761 aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat
     5821 tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt
     5881 tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg
     5941 aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa
     6001 cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta
     6061 atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga
     6121 tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga
     6181 ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag
     6241 ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc
     6301 agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa
     6361 aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa
     6421 aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag
     6481 ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc
     6541 attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat
     6601 tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg
     6661 aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat
     6721 taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt
     6781 tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac
     6841 aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag
     6901 cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag
     6961 ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt
     7021 acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag
     7081 actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa
     7141 atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa
     7201 atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa
     7261 aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga
     7321 gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt
     7381 gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt
     7441 aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca
     7501 caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa
     7561 aagaaaacag tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta
     7621 tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat
     7681 agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag
     7741 ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct
     7801 ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc
     7861 ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca
     7921 gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa
     7981 atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc
     8041 cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg
     8101 tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa
     8161 acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa
     8221 accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag
     8281 aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt
     8341 ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc
     8401 tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca
     8461 ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat agcaaattac
     8521 aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa
     8581 aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc
     8641 gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg
     8701 aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat
     8761 actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa
     8821 atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat
     8881 acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag
     8941 caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata
     9001 tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa
     9061 aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa
     9121 cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa
     9181 acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa
     9241 acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta
     9301 caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt
     9361 tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat
     9421 attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc
     9481 attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt
     9541 tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc
     9601 agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg
     9661 agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt
     9721 gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc
     9781 aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat
     9841 acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga
     9901 gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt
     9961 tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat
    10021 cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga
    10081 aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat
    10141 gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa
    10201 agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta
    10261 cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg
    10321 tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat
    10381 ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc
    10441 aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca
    10501 agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc
    10561 ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca
    10621 attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca
    10681 agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc
    10741 ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat
    10801 ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca
    10861 atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc
    10921 gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag
    10981 cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt
    11041 taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct
    11101 tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa
    11161 ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg
    11221 ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag
    11281 aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt
    11341 tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg
    11401 ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc
    11461 ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg
    11521 gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat
    11581 tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg
    11641 atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg
    11701 atgtaaatat cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga
    11761 cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta
    11821 gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac
    11881 caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg
    11941 ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat
    12001 tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc
    12061 gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag
    12121 caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta
    12181 gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg
    12241 aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt
    12301 cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta
    12361 aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag
    12421 cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa
    12481 catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa
    12541 atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat
    12601 aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat
    12661 gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa
    12721 ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa
    12781 aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga
    12841 gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa
    12901 tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa
    12961 aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt
    13021 aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac
    13081 ctctacacaa cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta
    13141 ttctgaagct atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt
    13201 tcatttagta gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta
    13261 tgcactagat ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg
    13321 gatggagatt gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta
    13381 agaggaggaa ctacgattta tactattgca ctattacttc atttacttca acgggcagcc
    13441 tatttctata gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat
    13501 caatcagggc taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat
    13561 cgtctgcctt tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg
    13621 aacttagccg tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat
    13681 tgattgaagc aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca
    13741 aatgtatacc agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg
    13801 attgaacagg ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa
    13861 tcctatattt tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc
    13921 ggatatgaac aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta
    13981 gaggaagatt ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta
    14041 agtcatggga aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc
    14101 gatatcatct cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt
    14161 gttgctgcac aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac
    14221 acagaaatga ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg
    14281 cgactagaca gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg
    14341 atcgaagctg gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt
    14401 gaactattag atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag
    14461 gggaagacga aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa
    14521 aaggtggttt actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa
    14581 aaatgcgggg atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact
    14641 atgaggaatt gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa
    14701 aacttgagta cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag
    14761 atacggctga taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg
    14821 cacaaaacaa agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa
    14881 ctgatgcaga agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc
    14941 ttgcgacaga aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct
    15001 tgatgcttga gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac
    15061 cattctccag ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc
    15121 ttgacaatga aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga
    15181 cggcatttga tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt
    15241 agaacagaaa agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag
    15301 acactgcatg gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag
    15361 cagcggctga cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt
    15421 gggtggtacc acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa
    15481 caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa
    15541 aattccctat gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag
    15601 aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc
    15661 atgatggacc tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca
    15721 gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag
    15781 gatgggatac acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta
    15841 aagaaatgtc catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag
    15901 ataagcaacg agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg
    15961 ttacattaga tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa
    16021 aaagctatat ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt
    16081 tagcggaagc tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca
    16141 atgtcaaaga cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa
    16201 caccatggac attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat
    16261 tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat
    16321 cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat
    16381 atatgactgc aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg
    16441 ttactttaga cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact
    16501 atatcgctgg tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt
    16561 ttacagaaga agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca
    16621 ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc
    16681 ctcacgattg gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat
    16741 ctatcgataa attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc
    16801 catggggtaa aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc
    16861 gtcagcgtgc atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca
    16921 tcacaccaga aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct
    16981 ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta
    17041 atggcgaatt tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc
    17101 atgaagctgt tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag
    17161 gatcagacca atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg
    17221 gtgtagcacc atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta
    17281 agatgagtaa atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg
    17341 cagatatcct tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta
    17401 tggatatctt aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct
    17461 tattagcgaa tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac
    17521 gttctgtaga caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag
    17581 aagggtacga caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg
    17641 ttgatctatc gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag
    17701 atgattatca acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa
    17761 aattattgac tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag
    17821 aagaggagta cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag
    17881 aattgatgga tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg
    17941 aagaagcaag aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc
    18001 caaaacaaca agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga
    18061 tcgtttcacc agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta
    18121 tggtatttga tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct
    18181 gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt
    18241 gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt
    18301 aaaagggaaa agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt
    18361 aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt
    18421 tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc
    18481 ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact
    18541 tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg
    18601 taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt
    18661 tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt
    18721 ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg
    18781 acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct
    18841 ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta
    18901 tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct
    18961 tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga
    19021 acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc
    19081 ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct
    19141 acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag
    19201 cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag
    19261 cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg
    19321 atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct
    19381 ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg
    19441 atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc
    19501 gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag
    19561 tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca
    19621 tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc
    19681 aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga
    19741 gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa
    19801 gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca
    19861 tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg
    19921 ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat
    19981 aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt
    20041 aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat
    20101 aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc
    20161 ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt
    20221 ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta
    20281 aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt
    20341 gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt
    20401 tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga
    20461 tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat
    20521 cttctcgatt tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag
    20581 tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac
    20641 gtttttacaa caaaaaacga aagatgatag agaagactca ttctttcgtt ttatgatttg
    20701 tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt cgattggtgt
    20761 tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac tggaaataaa
    20821 tgtctgattc acttttctaa aatccattga tttcggcagt ccattacgac gcagaatccc
    20881 gttagaattc tcgtttaatg ggcgttgag
//
LOCUS       DANKHK010000040        20704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000040 DANKHK010000000
VERSION     DANKHK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            352..1041
                     /locus_tag="PW109_001403"
     CDS             352..1041
                     /locus_tag="PW109_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511239.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="PW109_001404"
     CDS             1028..2236
                     /locus_tag="PW109_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2511240.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="PW109_001405"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="PW109_001405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2511241.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PW109_001406"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PW109_001406"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2511242.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="PW109_001407"
     CDS             complement(6339..7034)
                     /locus_tag="PW109_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2511243.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PW109_001408"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PW109_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2511244.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="PW109_001409"
     CDS             complement(8233..8424)
                     /locus_tag="PW109_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2511245.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="PW109_001410"
     CDS             complement(8445..9335)
                     /locus_tag="PW109_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2511246.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="PW109_001411"
     CDS             complement(9322..10089)
                     /locus_tag="PW109_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2511247.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PW109_001412"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PW109_001412"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2511248.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PW109_001413"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PW109_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2511249.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PW109_001414"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PW109_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2511250.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="PW109_001415"
     CDS             14660..15043
                     /locus_tag="PW109_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511251.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="PW109_001416"
     CDS             complement(15211..15969)
                     /locus_tag="PW109_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2511252.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="PW109_001417"
     CDS             complement(15987..16805)
                     /locus_tag="PW109_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2511253.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PW109_001418"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PW109_001418"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2511254.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PW109_001419"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PW109_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2511255.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="PW109_001420"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="PW109_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2511256.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="PW109_001421"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="PW109_001421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2511257.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5928 a         4220 c         3800 g         6756 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccaatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gttt
//
LOCUS       DANKHK010000041        20117 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000041 DANKHK010000000
VERSION     DANKHK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            204..1970
                     /locus_tag="PW109_001422"
     CDS             204..1970
                     /locus_tag="PW109_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2511258.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1985..2422
                     /locus_tag="PW109_001423"
     CDS             1985..2422
                     /locus_tag="PW109_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2511259.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2435..3586
                     /locus_tag="PW109_001424"
     CDS             2435..3586
                     /locus_tag="PW109_001424"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2511260.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3648..4346)
                     /locus_tag="PW109_001425"
     CDS             complement(3648..4346)
                     /locus_tag="PW109_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2511261.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4339..5691)
                     /locus_tag="PW109_001426"
     CDS             complement(4339..5691)
                     /locus_tag="PW109_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2511262.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5914..7314)
                     /locus_tag="PW109_001427"
     CDS             complement(5914..7314)
                     /locus_tag="PW109_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2511263.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7475..8212
                     /locus_tag="PW109_001428"
     CDS             7475..8212
                     /locus_tag="PW109_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2511264.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8337..8816
                     /locus_tag="PW109_001429"
     CDS             8337..8816
                     /locus_tag="PW109_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2511265.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8813..9580
                     /locus_tag="PW109_001430"
     CDS             8813..9580
                     /locus_tag="PW109_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511266.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9810..10379
                     /gene="lepB"
                     /locus_tag="PW109_001431"
     CDS             9810..10379
                     /gene="lepB"
                     /locus_tag="PW109_001431"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2511267.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10483..11085)
                     /locus_tag="PW109_001432"
     CDS             complement(10483..11085)
                     /locus_tag="PW109_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2511268.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11191..11660
                     /locus_tag="PW109_001433"
                     /pseudo
     CDS             11191..11660
                     /locus_tag="PW109_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11766..12350
                     /locus_tag="PW109_001434"
     CDS             11766..12350
                     /locus_tag="PW109_001434"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2511269.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12353..13426
                     /gene="prfA"
                     /locus_tag="PW109_001435"
     CDS             12353..13426
                     /gene="prfA"
                     /locus_tag="PW109_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2511270.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13419..14258
                     /gene="prmC"
                     /locus_tag="PW109_001436"
     CDS             13419..14258
                     /gene="prmC"
                     /locus_tag="PW109_001436"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2511271.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14251..14430
                     /locus_tag="PW109_001437"
     CDS             14251..14430
                     /locus_tag="PW109_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511272.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14452..15477
                     /locus_tag="PW109_001438"
     CDS             14452..15477
                     /locus_tag="PW109_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2511273.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15652..16896
                     /locus_tag="PW109_001439"
     CDS             15652..16896
                     /locus_tag="PW109_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2511274.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17049..17996
                     /locus_tag="PW109_001440"
     CDS             17049..17996
                     /locus_tag="PW109_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2511275.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18130..18759
                     /gene="upp"
                     /locus_tag="PW109_001441"
     CDS             18130..18759
                     /gene="upp"
                     /locus_tag="PW109_001441"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2511276.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18898..19140)
                     /locus_tag="PW109_001442"
     CDS             complement(18898..19140)
                     /locus_tag="PW109_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511277.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19261..19333
                     /locus_tag="PW109_001443"
     tRNA            19261..19333
                     /locus_tag="PW109_001443"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19294..19296,aa:Thr,seq:cgt)
     gene            complement(19452..20090)
                     /locus_tag="PW109_001444"
     CDS             complement(19452..20090)
                     /locus_tag="PW109_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2511278.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         6843 a         3587 c         4091 g         5596 t
ORIGIN      
        1 gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa
       61 ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca
      121 aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc
      181 aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa
      241 aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag
      301 cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg
      361 aggctttcgc taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt
      421 atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga
      481 tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc
      541 ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt
      601 ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag
      661 gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag
      721 agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa
      781 tcttattttt gaataatgca atcaatcatg ggattttgac accattggga actgaacaag
      841 tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg
      901 gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag
      961 ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga
     1021 aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat
     1081 tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga
     1141 tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt
     1201 cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg
     1261 aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc
     1321 ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg
     1381 gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc
     1441 aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta
     1501 ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag
     1561 tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc
     1621 agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca
     1681 tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg
     1741 ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag
     1801 tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag
     1861 cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca
     1921 atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa
     1981 cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga
     2041 agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata
     2101 tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc
     2161 gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt
     2221 tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt
     2281 gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta
     2341 ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac
     2401 ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg
     2461 aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt
     2521 cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga
     2581 attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca
     2641 aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat
     2701 tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga
     2761 aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc
     2821 agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata
     2881 tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc
     2941 tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa
     3001 taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg
     3061 caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg
     3121 ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt
     3181 tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca
     3241 tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac
     3301 acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt
     3361 gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa tggggattat
     3421 ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca
     3481 tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg
     3541 gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa
     3601 aaggggaatg tgacgaaatt tttgtcacat tccccttttt cgaataacta ggctgtttgt
     3661 ccttcaagat agggcagatc caattcttta ccgtaacgtt gttctaaagc taggcggatc
     3721 aaacgagctg ccaggttctc atttcttgca aggataggtc catggaaata agaaccgaag
     3781 acatttcggt aaatgacacc ttcagattgg tcttcaccat tatttccttg accttgtacc
     3841 actttcccca aaggtcgttc accttcacct aagaaagtcc gaccattgtg gttttcaaat
     3901 ccataataag tttcattgaa ttcttcgtta tggataacga tatcaccgat aaaccggtta
     3961 ttttcctgac ttaacgtgta gtgatccagt gcactgattc cttggatctt ttctccatta
     4021 gctcccatgt agtagtgacc tagcaattgg tagccaccac aaatggctaa catgacgcca
     4081 tcattttcga tatagttgat caaagaatct ttctttttct ggatgtcttt tgaaatgatt
     4141 aattgctcaa agtcttgacc accgccaaag aaaaccaagt catatttatc agcatcaaaa
     4201 ggttcatgta tactaacgat ctccgtatgg caagaaaccc ccattttttc tgctatatat
     4261 ttcaacatca aaaggtttcc gttatcacca tatgtgttca tcaaatttcc ataaaggtgg
     4321 gcgatattga gttcataatt agccacggtt cattcctcct ttgataaatc cttgagcagc
     4381 tagttcttta cgaagctgca atacagcagt gtaagtagct aaaatataga catgctcagt
     4441 tggcaattct ttgatttttt cgattacttc tggtaattct tttgtttctg tcaatttatt
     4501 ttctggaatc ccggcaactt ttagacgtaa agtcatatct gtatgacggt ctcctccaga
     4561 aataacagct gggatatcca tatcggcaaa gttttcaaaa ttaccatccc aaatccagct
     4621 gacatcgatt ccgtctgcgt aattggcatt taataaagcc actaaagaaa atggataagg
     4681 agctagacca atcatatcga ttacttgatt gatacctaca ggatttttga caagtactaa
     4741 tgtacatttt ttcccattga tattgatgac ttcttgtcgg ccgaatactt tctcgtcata
     4801 tcctaaacct tgtttgattt tatcaggatc gacattgaag tactctgcca ctgcagtagc
     4861 tgctaaagca ttgtagacat tatacatacc gccaacttcg attccgtatt catgaccatc
     4921 gatcacgaaa tctgctgaca cattgtccat ccgtaccata tcagttaatc gataatccaa
     4981 ttcaggacgt ttgaagtcac aatttggaca gtaatatttc cctaagtttg cataagtgat
     5041 cattttataa tgcaagatgt gatgacaatt tggacataag acaccgtctg tattgtaatg
     5101 agccatttgt tcacgatctt cttcatggtc aaagccataa tattttctag gattgactgt
     5161 ttcgatagaa ttgaagattg gtgaatcacc gttactgata atcgttgcat ttggtgactt
     5221 tgcggcaccg tctacaatca ttttataggt cgtatagatt tctccatacc gatccatttg
     5281 atcacggaaa atatttgtaa ataagaacag ttctggtttc atatattctg tgactttgct
     5341 caaacttgct tcatctattt ctaggacagc aaactttttc tgtccttttt ttggttttgc
     5401 ctgcaagaaa gtcgatacaa ttccctgtac catattcgca ccagtagggt tggttagaac
     5461 ttcatcgaat tgctggcgca aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt
     5521 tgttccggtg acaacgacga cttggtagtc ttttgccaaa ctatctagta tatgtggatc
     5581 gactttcagc gctagtttac ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc
     5641 ccattgcgac gtttttccaa ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc
     5701 tccaaaaacg atttgctgac gaataaattc aactaatcat accatatgtt gaaaaaaagg
     5761 ttaaattgct tttcttattc ttaagaaaag gttacggctg aaagtgcatg aaatcggaca
     5821 aaatcgcatg atttaacaaa aaaacagctt gaaacaaaat gattgtttca agctgctgtg
     5881 attttattat tccctcttac ctatattccc attttaaccg ataataattt tctcgcttgg
     5941 atattcatag cctagatctc gtgtttcttt tggcacgaat aacatcaatg tatatagcat
     6001 tccgatccgc ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc
     6061 tccggtaatc cctaaagata aaccagttgt tccaaacgca gaagttactt cgatgatgat
     6121 cgagatcagc gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga aacacatacc
     6181 aagagaaagc ataaagacaa cgactgattt tcgcacatca tcttgatcga ttctccgtcc
     6241 aaaaatattg atgttatctt cacttttgag gaaggaatat agatacaaac cgatgattgc
     6301 tacagtagtt gttcgaatcc cgccgcctac agaactagga ctgcatccga taaacatcag
     6361 taaagaaaaa atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc
     6421 agcattacga gtcgtaatcg aataaaacat cgaattgatc catttgacac tcatattgga
     6481 atcttggaat aaatggtcct tttccaatag ataaatcaag acagttcccg aaacaaacaa
     6541 gataacaaaa gctaacacag ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc
     6601 attggaacgt ttgtataata gccattcccg gcactccatc aatacaggaa aaccaattcc
     6661 tccgacaaaa atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg caaagggctt
     6721 gatggaatcc ccagtgacgt caaacccaga gttcgtgaca gccgatatcg cctgataaaa
     6781 accgtaaaaa acggcatcac gccaacgatc aaaatagcct ctgtaataaa aataaataga
     6841 aaagaatgtc ccaaaaagta attgaaacca gattaaaata gcaaaagtga tccgaatcag
     6901 tcggacgata ccagacaaac gcggttgatt catatccgtc atgatcagct gtcgctgttt
     6961 cagtgtgatc cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca tgatccccaa
     7021 accaccaact tgaaataata tttccagcag tataataccg ttatcattga acacagaatt
     7081 gatatcaaaa gtcgatagtc cagtcacact cactgtactg attgccatga acagcatgtc
     7141 cacaaaagaa acatggctat ctggttcgcg aaaaatcggc atataaaata gaacgagtga
     7201 taagaccgtc atcaatatat aataaaaaac gatgatttgg atcgatgaga aatgattggc
     7261 agcaaacttc tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat gcacagtact
     7321 atcctcctga caatagtcgt ttctaccagt atacaagggg aattaggaat actcaaagaa
     7381 attatcaaac agataacaat acagctttcg attaaaatag aaatggctta taataaggaa
     7441 aatcctagaa aaaagaagga ggaggaataa caaattgatt aatgctcaac aaatcgtaga
     7501 aaaaatgaaa aatcaaaaaa taaattatga tacagttctg aaaaaaatga tcatgcaatg
     7561 ggagaaagaa gaagtaaagc ctaagatatt aatacataca tgctgtgctc cttgcagtac
     7621 gcacacattg gaattcatgt ctcaatatgc agagatcacg ttattttttt caaattcgaa
     7681 tattcatcct aaaagtgagt atttgcgaag attacatgaa caaaaacgat ttgtagacca
     7741 atttaatgag aaaactgacc atcatgttgc gctaatcgtt gatgaatacc gtccaaatga
     7801 attcaccaaa atggttttaa caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg
     7861 cagtgcatgc ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag aactaggata
     7921 cgattacttt ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt tgatcaatca
     7981 gatcggaatg gatatccaaa agatttacgg gacacaatac ttgccaagtg atttcaagaa
     8041 aaacaaaggc tatgaacgct ctttgcaaat gtgcaaagag tatgatattt atcgccaatg
     8101 ttattgtggt tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg
     8161 ggaagcaaaa gcttacttaa aaacatcaac cgtagttttt gaggaaaatt aaagaaatct
     8221 ttcggaaaaa cgtaagaaaa aaataaagaa tttatggtag aaataaggta gactgtataa
     8281 tttgtccgta gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga gataagatgc
     8341 gaaaagtagc acctattttg aagccaatcg ctgtattaac aatctcgacg attgtcgcga
     8401 ctactacagg tattgtgaat agttccagct accgtgcacc aagttcaagt tctagtactg
     8461 tagaagaaac aacaccaact acttcagtct cgacatctga accaacaaca acggaaagtt
     8521 tgaatgaaac cactacgaca agtacatggg acgatacaac aactagttcg tcgtatgatg
     8581 agacatatac aactgacagc tcaagcgaaa caagtaatac ggtaccagat ggcactccat
     8641 cggattcaac caatccaagt acaagtccgg atacaagtgc tagctccgaa acgataccaa
     8701 gcagtacgac tgtagatgat aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa
     8761 accaagattc aggaaatacc caaacgtata accagcaagc aggtgattct caatgacatt
     8821 tttactagac cttatcaacg gggtgcataa attccttggc tatttagaca tcagtcctaa
     8881 atatcttaac cgtggatata cgattttaag tgtgattccc acgctttatt tattgagaat
     8941 tgtttatgga ctttggcaaa accaaaacta tcttcaattt ttcttatatg gacttgcatt
     9001 tctagtgctt gtttatttta ctgttttaaa tgtattttat tactttttag ataaaaacac
     9061 gaaagccgat gtcactcagc tttttgtgaa gtatttacct gatgaagcgt tcaacatcca
     9121 gacagaagaa tcgatagata atggaaattc gatcgataaa gtgaatacac aagaggtcat
     9181 ggtttcttat gacgaagatt atcaattgaa attagccgag aatatgcggt atttgattgg
     9241 aaatggtgag atcaaaacaa atgaactcgg tcgcatggat ggatttctgg tcgatagaaa
     9301 tacattgtat ccttattact atgtcaagaa aacaggagag aaagagtatg ttctaaatat
     9361 tggacgaagc tactcggaca tggaaaaagt agggaccatt cgacttgact caacagaaga
     9421 cacattggaa cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag acgggttacg
     9481 ctatcatgag ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc
     9541 agtggtttat agtcgtagcc aaagaagaag acattcataa tgtgatgaaa cgccgtgtat
     9601 cagtggttgc gataaaaacg tttattcgga ataaggggga tgtgacacgt cttttgtcac
     9661 gttccttttt tatataacga gaaagataag acgaacacat cacttattct ttcctttcat
     9721 ttataaacat tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac gtaataaata
     9781 acgaggaaaa tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat tgggggattt
     9841 tgatccttgt attagcggct attttattag ctagagtgta cgtgtttaca ccagttactg
     9901 taaacgggca ttcgatggac cctacattaa gtgatggaca acgattgatt tcatctaaga
     9961 tctcaaacta tgaacgaatg gatattatta ctacaaagga accgggggat gaagaacgga
    10021 tgattgttaa acggatcatc ggtatgcctg gagatacagt caagatggaa aatgaccagt
    10081 tgaccattaa tggcaaaaag tatgatgaac cttatctgga tgagttcaaa aaagaatttt
    10141 cagaagataa gttacaagga gaatatgctt atagctcagg attccaagca caagcagaaa
    10201 gttcatccac ctttacgagt gactttgaat atactgtgcc aaaaggaaag tatcttgttc
    10261 taggcgataa ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc gacaaagata
    10321 tgatccaagg aaaagttgtt ttccgttatt ggcctctttc agaaattaaa atcatgtgat
    10381 gaaaaaataa aaagaagctg tgacatgttt tttgtcacag cttctttttc cgaaaattcc
    10441 ttcttatttc ttggagttgc tcactttttt tgcaatctca ttttaagctt tttggctgat
    10501 ggataagaaa agcgaagcgt ttttgttcat aaatagcttt tccccacgat ggaaggtatg
    10561 actagtagca gagacaaagc ccgttttttc caatcgatta ttcaattctt cttggctaaa
    10621 gccattatgg actttcggat gactaatttg ttcattcttg tcaaagtcta cgatgatcaa
    10681 ttgaccgcca ggagctaaaa tttcataaag ttttgtaaga atattctccg tatcaggaat
    10741 gtgtaaaagt acaagtgata gaaggatgag attagcttgg acagcaggaa attcgacaga
    10801 agcatcagcg tggatcgttc ggacattttt cagatctgct gcttggatct tttcttctgc
    10861 cattttcagc atagtttctg aagcatcggc aatgatcagt tctttgaatc tttccgcaag
    10921 cggtaaacta acaagcccag ttccgcctcc gtaatccagc aggacttttt ggtggctgtc
    10981 gcgcggtaga taagtagtta gttcttcgcg aataatcttg gctaatgctt gacggtcagg
    11041 ggaatcataa tggttagcga tggaatcaaa aatatttttt gtcatcaaat agctccttac
    11101 gtaaaacttt gaaattttaa cttattatag cacgtgaaaa gaaagaaagc atgctatact
    11161 caattcaaaa agggagaatc tagaaagaat atgccgatat cgtttgtaaa agatagagag
    11221 gaaaaaggaa agtgtgtccg agaaatatta ctagacttgc ctgagtggtt tgggcttcct
    11281 gaatcaacgg aaaaatatat cgaggaatca agtaaactac ctttatggtg tgaaaaaaga
    11341 aaagaagaat atttaggctt tatcactctt tcacagacaa gtgaagatac tgccgaaatc
    11401 tattctattg tatgggaata aaaaaagact atcatcacca aggattagga acaaaacttt
    11461 ttaaagaagc agaaggttac gcagcaaaac attacaaata tttgcaagtg aagactgtag
    11521 acgaggggca ctactctata tatgatcaaa ctatttgttt ctacgaatca ttaggatttt
    11581 caaggttgga agtttttcca aatttatggg atgaatggaa cccatgttta gttcttgtca
    11641 aaaaattgga acaaaagtga gcaatagatt taatactaat ctgtgcttga tgaaaattct
    11701 cactgtaaaa aatgctgaaa atcaagtata ataccatagg aacaaactgg aaggtgaaaa
    11761 agagcatggc acaactattt tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga
    11821 tcctaaaagt cgcacataat tatgaagaac aaaataagcc agttgtctta atgacaagcg
    11881 gtattgatac aagagatggt ttaggcgttg tttcaagccg aatcggactg aaacgtaatg
    11941 cgatgccgat atttgaagaa acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac
    12001 cctattgtgt gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta
    12061 cgagaattgt ggacgaattg gatattccgg ttatggcatt tggtttgaaa aatgattttc
    12121 gaaacgagtt atttgaagga tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa
    12181 tgaaaacaat ctgttggttc tgccacaaaa aagcaatcat gaatttacat tatattgatg
    12241 gaaaccctgt ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct tattaccccg
    12301 tttgtcgaaa acattacttt cacccatcaa tgattacaga aggagaataa gcatgtacga
    12361 tcaattacaa gcaattgaag accgttatga agagcttgga gaacttttaa gtgacccaga
    12421 agtgatttct gatacaaagc gttttatgca gctttcaaaa gaagaagcaa acacgcgcga
    12481 aacagtcgaa gtttatcgtc gttataaaga agtagtacaa ggaatcaccg atacagaaga
    12541 actgcttggt gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag aattatctga
    12601 attaaaaaaa gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt taccaaaaga
    12661 tccaaatgac gataaaaata tcatcatgga aatccgtgga gcagcaggcg gagacgaagc
    12721 agcattattc gctggcgatc ttttcaatat gtaccaaaag tatgctgaag cacaaggctg
    12781 gaaaacagaa gtgatggaag ctaacgttac cggaatcggt ggatataaag aagtgatcat
    12841 gatgatcacc ggagacaacg tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt
    12901 ccagcgtgtt ccttcaactg aatcgcaagg gcgtatccat acttccaccg caacagttgt
    12961 cgtgatgcca gaagctgaag aagtagagat cgatattgaa gacaaagata tccgtacaga
    13021 tatctatcat gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg
    13081 tttgactcac ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa
    13141 gaaccgtgaa aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga
    13201 agcacaaagc gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga
    13261 acggatccgg acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac
    13321 tatccaaaaa ttagatcaga ttttagcagg gaaattagat gagatcgttg atgcattgat
    13381 catgtatgat caaacatcaa aactggaagc tatgcaaaat gggtaaaatg acctatcgag
    13441 aagtcctgtc acgggcttct tcttttttag aggatcatgg gaaagaagca tatagtatcc
    13501 aatttttatt tttggaacga aaacaatgga aaaaacttga ttggctcctt catatgaacg
    13561 aagagatttc agaagaagaa cagcggttga tcgaaaccga tctgcgactt ctgcttgcag
    13621 atcatccacc tcagtatttg ctaggatatg cagattttta tgatcatcgg ttaaaggtaa
    13681 ctgaagcaac actgatccct cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg
    13741 agacgcctgg tgtaccgtta gaagtgatag atattgggac agggactgga gcaattgcta
    13801 ttagtttgaa agcagcaaga aaaaattggc atatatctgc tgtggatctt tcagaagaag
    13861 cattagaagt agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt ttttatcatg
    13921 gagatacatt aggaccagta atggatcagc aatttgatgt gattatttct aatccgccgt
    13981 acatcagtcg gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa
    14041 tggccctttt tgctgagaac gatggattag ctatttatga aaaaatagca gttgaagcac
    14101 caagcgtact aaaatcaaat ggaaagattt ttttagaaat cggatttcgc caaggtgaag
    14161 cagtaaaaaa aatatttcag caagcatttc cagataaaaa agtggcgata aaaaaggatc
    14221 tttttggcaa tgaacgaatg atccaagttt atggttgaag ttgtggatga aaaatttttt
    14281 atttgttttt ttattataaa aaaacactta tttgacaagg atgtaaacga ctttttggtt
    14341 tatcaacaag tttatccact attgacaaat agattatcca caaacagtgg aaaacttgag
    14401 gataaactca gacaaaataa gaatgtatga aaaaggggtt gtggataaac tgtggaaacg
    14461 aaacgatatg atgaaacaga attgcaagag gcagccgaag cactgaaaga aggagaactt
    14521 gttgcttttc ctaccgaaac agtatacgga ttgggcgcca acgctctttt accaaacgct
    14581 gtcaagaaag tgttttctgt aaaagggaga ccgcaagata atccattaat cgtccatgtc
    14641 gcctcttttg aacaagtaaa agagtatgtg gacaactttc atccggcagc agagaagatt
    14701 gtgaagaact tctggcctgg accattaact ttgatattta agatcaaaaa agacactttg
    14761 ccctcagttg tgacgggagg attatccaca gctgcatttc gtataccgga taacaaaaaa
    14821 acactagaag taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg
    14881 ggaaaaccaa gtccaacgac tgcagatcat gtatatcatg atctacaagg aaaaattacc
    14941 gggattattg atgatggagc tacgcgaatt ggtgtagaat ccactgttct tgatttaagt
    15001 gatccaacag ccatgcctat gattttacga ccaggtgcag taacgaaaga acaaatcgaa
    15061 gcagtcatcg aatctcctgt tgctattgat cagcatcttg taaaagaaaa tgaaacacca
    15121 aaagcccctg ggatgaaata caaacactac tcacccgata cacgtgttct gatggtcaga
    15181 gatggcgatt ggtcaactgc agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc
    15241 atagcaagcc ccgaaattgc ggatcaagtg cggacagata cagcagctgt ttatatgtat
    15301 aacgacaact cagtagaagc ggcggctaaa ggattgtttg caggtctaag aggattagat
    15361 gaaccaacac ttggattaga tttgattttt gtacaagtgt atccagaaac tggattgggg
    15421 aatgcttaca tgaatcgctt gaaaaaagca gcaggacaaa attattttga aaaatgatca
    15481 attggttatt gtcgcattct tttagtatga tacaatgcta tcaagtaaat cttctatcag
    15541 tgaacgaaaa actaagcgta actaatcgta atagaagaga tacgaatatt tggcaatttg
    15601 tttttgatgg gaaaaataaa attgtaggaa aataaaaagg agctgttgtt tgtggtagat
    15661 tacaaaacgt ttgaccctga tctttgggct gcgatcgcca aagaagaaga aagacaagaa
    15721 cataatttgg agctgattgc ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa
    15781 ggaagtatct tgacaaataa atacgcagaa ggttatccag ggcatcgcta ttatggtggg
    15841 tgtgaatttg tcgatatcgt tgaaaattta gcgattgacc gtgcaaaaga attattcgga
    15901 gcgaaatttg cgaacgtcca gcctcactcc ggctctcaag ccaacacggc tgcttatctc
    15961 gctttggtag aaccagggga cacgatctta ggaatggatc tttccgcagg cggacatcta
    16021 acacatggat caccagtcaa cttcagtggc aaaacctatc actttgttgc ttacggtgtt
    16081 gatccgacaa cagaagttat tgattataat gtggtacgta ttttagctag aaaacatcaa
    16141 ccaaaattga tcgtagcagg tgccagtgct tacggacgaa cgatcgactt tgcgaaattc
    16201 agagaaatcg cagatgaagt aggcgcaaaa ctgatggtag atatggcaca tatcgcagga
    16261 ttagtagcag caggacttca tcctaatcca gttccttatg cagatatcac aacgactacc
    16321 actcataaaa cattgcgtgg ccctcgaggc ggcatgattt tgacaaacga tgaagcatta
    16381 gcaaaaaaaa taaatagtgc ggtatttcct ggtattcaag gcggccctct agagcatgtg
    16441 attgcgggta aagcagtagc attcaaagaa gcattagatc ctgcatttaa agaatatagt
    16501 gaacaaatca tcgccaatgc aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc
    16561 agagtcattt caggggcgac agataatcat ctgatgttga tcgatgttcg cgagttaggt
    16621 ataaatggaa aagaagcaga gagcattttg gacagtgtga atatcactgt aaataaaaac
    16681 tcgattcctt ttgaaacact aagtccattc aaaacaagcg gtattcgaat cggcacgcca
    16741 gcaatcacta ctcgcgggtt caaagaagaa gatgctgtaa aagtggctga actagttgtc
    16801 aaagcattac aagcaaaaga tgataacgct caactagacg aagtcaaaac aggtgtacgc
    16861 gaattaactg aaaaatttcc gttgcataaa aaataaaaat ttaaattcca ttagtaaaca
    16921 gaaaagttca atgttgggct tttctgttta ttttttggga aaacggattc tttgacaata
    16981 tcggtgatgc tctctagtat atgaggtgta attttagtct tcacactaaa tctcagggag
    17041 gaaaagtcat gactgatcgt atctcgttag aaaaggaagc agtagcattc agtgatgcaa
    17101 atgcgcctca tccgcgtatc tatgaactag caccggaaga aggaagagtt ttactagaag
    17161 aagtacaaga atcacctgtg aacaaatatt ccgtcgatat tgaagacttg atggtggata
    17221 ctggcgaatg gggaaaactc aatattcgtc tcgtacgacc agaagggaat atggataaat
    17281 taccagttat tttttatatt catggagcag gctgggtttt tggcagtgct caaacccatg
    17341 ataaattgat cagagaactt gcggttcgta cgaattccgt cgttgtcttt ccagaatata
    17401 gtcgatcacc agaagcaaaa tatccaacag caattgaaca aaattatgct gtgttgcaac
    17461 agctgaataa attagccaag tcaaaaaatc tggatctcga gaatctaatc gttgcaggag
    17521 attcagtagg tggtaacatg gctacagtca tgacgatctt aacaaaagaa cgaaagggcc
    17581 tcccaatatc caagcaattg ctgtattatc cagtcacaaa cgctgatttt gatacagatt
    17641 cctatcatga attcggagaa aactattttt tgacaagaga aggaatgatg tggttttggg
    17701 accaatatac gacgaatgaa gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa
    17761 atataaatga actaaatgat cttccttcag caatgatttt aacgggagaa gcggatgtat
    17821 taagagatga aggtgaggct tatgcgcgca aacttcgtga tgcaggggtt cctgttacac
    17881 aagtacgctt ccaaggaatc atccatgatt ttgtcatggt aaatgctatg gatcagacaa
    17941 aggcaacgcg tgcagcaatg gcattatcta cttcttggat caagaaaaaa gaataagctt
    18001 tgcttgtccg caggaaaact tcttttcaaa aacggatttt cttaggaaaa atggcgagtt
    18061 tgactagtca atcacttgat tttgttttac aattaaaaag aaacgacaga acaaaggaga
    18121 gcttaaaaaa tgggcaaatt tcaagtgatc gatcatccat tgattcaaca caaactaact
    18181 attattcggg acaaaaactg cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct
    18241 atgttgatgg catatgaagt atcacgggat atgccattgg aagacatcgt gatcgaaaca
    18301 ccgatcactg aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat tattccaatc
    18361 ttgcgcgcag gtattggaat ggttgacggt atcttggaat tgattccagc tgctaaagtg
    18421 ggacatatcg gtctttatcg agatgaagaa acattggaac cacatgaata tttcgtcaaa
    18481 cttccagaag acattgatgc acgtcagttg tttgtagtag atccaatgtt ggcaacaggc
    18541 ggatcagcaa tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt
    18601 gtttgtttgg tagcagcacc tgagggagta aaagctttgc aagaagcgca tcctgatatt
    18661 gacatctata cagcttctct agatgaaaaa ttgaatgaac atggttatat cgttcctgga
    18721 ctaggggatg cgggtgaccg tttgtttggt acaaaataag taaatacgaa aaagggtgtg
    18781 acacaacttt tgtcacatcc ttttttggtc atttttaacg tttgatgcag aacagctttt
    18841 tcacagcttt cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca
    18901 aatctttttt agttctttgt agttaacgat tactacaccc ttactttgcg tcatcaagtt
    18961 cgcattttct gcagtttcat caatctcaac aacagcggaa tttttcaatt gctttgaaat
    19021 cgttccagtt tgcgacttgc cgtgaattgt gaacattacg tgagtattga tcttgaattt
    19081 ttcaccaatt tccgatggta cgatagttgt atcaaatttt ccaaaagttg ccattttcat
    19141 aaaacctgcc tttcacatca attataccac gttttatgaa aaatatcatg aaattaaact
    19201 aattttccat cggactcttt acaatcagcc aaatagagta tataatttct tttggataag
    19261 gccagcttag ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc
    19321 cctatagctg gcattaaaag agaagacaga tatatcaagg atttttaatc cattgataca
    19381 tctgtctttt tgtttaaagg aaataaagta tgctttctta gcaatagagc atgtattatt
    19441 tctataaaaa tttaggagaa taaaacgact agcaatatag ctgtgaaatc aaatatcaca
    19501 tgaactgccc aacttgtaat gatcgagttg cttttaatgg cagcttggtt gaataacaat
    19561 cttgccaccc cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt tccattatca
    19621 taagtactgt aatggataaa tccaaagatg attgctgaca agaaactagc gacaggaatg
    19681 ctgaacttac tagaaaaaat agcgagaaaa tacactgaga acaattcttc tccaagtatc
    19741 atgataggca aaataataaa taataacggg ctgaaagcct catttattgg attgcctgtt
    19801 aaaccccatt tcaaaatttg ttgcaaaaat accgagactg cggcactgat tactacattg
    19861 ataaaaaaat atttggctat atttttgaca ggagcgacag gtttcgagaa catttttttc
    19921 gtatcttttt taccaaaaac aagtgtaaat gctataaatc cagtgatagt taaagaaaaa
    19981 atgctaataa tggatggcgc aaatagcaac cctaacaaac cgagtacaac gacaatagtg
    20041 cccaatatcc cttttttaag ttctattttt tttcctaaaa tgttattcat gataataact
    20101 cctactattc agttttt
//
LOCUS       DANKHK010000042        19866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000042 DANKHK010000000
VERSION     DANKHK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(371..1315)
                     /locus_tag="PW109_001445"
     CDS             complement(371..1315)
                     /locus_tag="PW109_001445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2511279.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1613..2557)
                     /locus_tag="PW109_001446"
     CDS             complement(1613..2557)
                     /locus_tag="PW109_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511280.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSTDGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLNKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2777..3991
                     /locus_tag="PW109_001447"
     CDS             2777..3991
                     /locus_tag="PW109_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2511281.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYVTRSLSAI
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQLGWQ
                     YTFLIYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKHAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4013..5818
                     /locus_tag="PW109_001448"
     CDS             4013..5818
                     /locus_tag="PW109_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2511282.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TETGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLCIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYE"
     gene            5822..6028
                     /locus_tag="PW109_001449"
     CDS             5822..6028
                     /locus_tag="PW109_001449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511283.1"
                     /translation="MIQPNLDSFETLNCTLPIKDGKISLEKTKDKIVITSDRTGGTVK
                     MNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6111..7484)
                     /locus_tag="PW109_001450"
     CDS             complement(6111..7484)
                     /locus_tag="PW109_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2511284.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQMALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7510..7794)
                     /locus_tag="PW109_001451"
     CDS             complement(7510..7794)
                     /locus_tag="PW109_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2511285.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7796..9811)
                     /locus_tag="PW109_001452"
     CDS             complement(7796..9811)
                     /locus_tag="PW109_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2511286.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     THIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFDSFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDDPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9990..10295)
                     /locus_tag="PW109_001453"
     CDS             complement(9990..10295)
                     /locus_tag="PW109_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2511287.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10384..11415)
                     /locus_tag="PW109_001454"
     CDS             complement(10384..11415)
                     /locus_tag="PW109_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2511288.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNILVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11622..11810
                     /locus_tag="PW109_001455"
     CDS             11622..11810
                     /locus_tag="PW109_001455"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2511289.1"
                     /translation="MLIEEKLTKQELFTTTEKRIADYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKNGIQRI"
     gene            11791..12498
                     /locus_tag="PW109_001456"
     CDS             11791..12498
                     /locus_tag="PW109_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2511290.1"
                     /translation="MGFSGFKEFRFELGWEVHQLIAQFNQTIDPNFPFSSEDSTQQIA
                     KKMADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKL
                     NKFLIMAEEYSDSSWNAANLISEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGN
                     QHSEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLK
                     NKQELMQKGLLKEYRKT"
     gene            complement(12650..13243)
                     /locus_tag="PW109_001457"
     CDS             complement(12650..13243)
                     /locus_tag="PW109_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2511291.1"
                     /translation="MHNKIHNKERLLIWAFFLKQIQLTIILDHNFFDLLFFDWKWQRA
                     SSVLLEGTSYPTGGENTHAVISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQV
                     DQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQK
                     VSENKRFFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            13247..14032
                     /locus_tag="PW109_001458"
     CDS             13247..14032
                     /locus_tag="PW109_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2511292.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSSDCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTICATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(14105..14752)
                     /locus_tag="PW109_001459"
     CDS             complement(14105..14752)
                     /locus_tag="PW109_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2511293.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKIGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNKKWLELLRI
                     NHFSIQSSEEAVSFLYFSMSLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(14760..15446)
                     /locus_tag="PW109_001460"
     CDS             complement(14760..15446)
                     /locus_tag="PW109_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511294.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(15452..16525)
                     /locus_tag="PW109_001461"
     CDS             complement(15452..16525)
                     /locus_tag="PW109_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511295.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEEGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            16748..17422
                     /locus_tag="PW109_001462"
     CDS             16748..17422
                     /locus_tag="PW109_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511296.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            17427..18767
                     /locus_tag="PW109_001463"
     CDS             17427..18767
                     /locus_tag="PW109_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2511297.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            18856..19866
                     /locus_tag="PW109_001464"
                     /pseudo
     CDS             18856..19866
                     /locus_tag="PW109_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         6233 a         3698 c         3582 g         6353 t
ORIGIN      
        1 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
       61 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
      121 agatatgaat cattgtattt gccgttgcag aaggattttt taattaacca cagataaaca
      181 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
      241 tacatttttg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
      301 ataaaaactg cttaacagct atttcagctg ttaagcagtt ttaggatttt tagttcatat
      361 tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt gatttgctga
      421 acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca ccattttcgt
      481 caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca gttaaataag
      541 cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata cgtgcagtac
      601 tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa gcagcattac
      661 ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct ttttctacaa
      721 tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact tctgaatcac
      781 catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat ttcaatgcga
      841 tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca cgtgatgttg
      901 gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt gaagcaacaa
      961 cgataatacc gtcgaatcct gatcccatga tttcttttac gatttgacgc atgattgacg
     1021 catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata ccagctgtga
     1081 taacaacgat atcagcatct ttacactctt catagccacc tgaccataca gccacatttt
     1141 catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct ttttcttgat
     1201 tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca taagcgatac
     1261 ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt ttcatgaata
     1321 aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt gtttattcat
     1381 tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa cgaattcctg
     1441 aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata atacaaccaa
     1501 aagtttagaa ctgaaaataa atccaaaaaa aaattcgctg caacgatata ataagacagc
     1561 aaccgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1621 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1681 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1741 tcagttccaa cgcaaagtcg aattttttat ttagaaccag ttcttgaaag gaagcatcaa
     1801 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1861 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1921 tttctgcgca tccatctgtt gatgaaaagc tttgttgttc taataaatga gtcaattcga
     1981 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     2041 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     2101 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt gatcaaaaaa gaagaaagta
     2161 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2221 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2281 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattgata atttgtaaac
     2341 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2401 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatct cctcggtgga
     2461 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2521 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa agtgtttaat tcagctagtt
     2581 ccaattgact tcctcctttt tgtaagtggt ttctttcgct tattagttta cgctgtactg
     2641 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
     2701 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
     2761 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
     2821 ctctcttatt ctgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
     2881 ttatgtcacg cgttctttat ctgctattga attattaacg actgctccct cctttatggt
     2941 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
     3001 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
     3061 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
     3121 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
     3181 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
     3241 acagttaggt tggcaatata cctttttaat ctacgccatc agcttaccta ttcttgtctt
     3301 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg gcacagcccc
     3361 aacaagacaa gttatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
     3421 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
     3481 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
     3541 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
     3601 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
     3661 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
     3721 ccaagtaagt atccaagcgc ccaagcacgc ggagagtttc aatacttcaa tcttattggt
     3781 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
     3841 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
     3901 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
     3961 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
     4021 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
     4081 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
     4141 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
     4201 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
     4261 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
     4321 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
     4381 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
     4441 agacccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagac
     4501 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
     4561 tctggatgac cacatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
     4621 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
     4681 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
     4741 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
     4801 gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt tgttttttat
     4861 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
     4921 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
     4981 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
     5041 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttatgca tcgtctaccc
     5101 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
     5161 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
     5221 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
     5281 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
     5341 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
     5401 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
     5461 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
     5521 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
     5581 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
     5641 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
     5701 cagtatgtat ggagatcctt atggaaaaag cttgtgccat gcatggggag caagcccaat
     5761 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaataatt
     5821 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
     5881 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
     5941 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
     6001 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
     6061 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttac ttaattctgt
     6121 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
     6181 aaaaagaaag cgatgattcc aactgtcaag gccatttgac cgtattctcc tagtttccca
     6241 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
     6301 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
     6361 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
     6421 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
     6481 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
     6541 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
     6601 ttagcaatcc cttggaatgc tggaacgatt tctgccaata ccattcgaac cccttgaagg
     6661 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
     6721 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
     6781 tatacgattg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
     6841 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
     6901 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
     6961 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
     7021 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
     7081 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
     7141 gtgaaccgag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
     7201 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
     7261 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattagacca
     7321 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
     7381 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
     7441 ggctgactga cgattgacac aaacaaattc aataatttat ccattgatag ttcctccctt
     7501 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
     7561 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
     7621 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
     7681 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
     7741 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
     7801 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
     7861 gaagattttg tttattagaa agtagctttg ttaaatttgc caacgcccga agatgcgcct
     7921 cattatcgat agcagctaaa cagataaaca aactaatggg atgggcagga tcatcaagca
     7981 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcatttacg ccatcttctg
     8041 gacgagcatg aggtaatgca atgtggtcgc caatatggat aaacgcacca tactgctcca
     8101 cgcgctcaat catagctttg atataacttt cttctatttt attttctgat aatagcggct
     8161 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
     8221 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
     8281 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
     8341 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
     8401 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
     8461 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
     8521 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
     8581 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
     8641 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
     8701 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
     8761 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
     8821 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
     8881 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaatca aattctacgg
     8941 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
     9001 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
     9061 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
     9121 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
     9181 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
     9241 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
     9301 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
     9361 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
     9421 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
     9481 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
     9541 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
     9601 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
     9661 tctttatacg gatatgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
     9721 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
     9781 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
     9841 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
     9901 cgactgtacc gaacgacttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
     9961 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    10021 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    10081 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    10141 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    10201 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    10261 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    10321 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    10381 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    10441 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    10501 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    10561 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    10621 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    10681 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    10741 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    10801 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    10861 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    10921 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    10981 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    11041 agaatcgaac cgtctgtttt ggtaacattg gataaatagc cctttttctt ttgctaatgc
    11101 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtatattctt
    11161 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    11221 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    11281 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    11341 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    11401 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    11461 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatt
    11521 attcggtttg atgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    11581 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    11641 taacaaaaca agagttgttt accacgacag agaaacggat tgccgattac atccgtagga
    11701 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    11761 attcagctat cattcgttta tgcaaaaaaa atgggattca gcggatttaa agaattccgt
    11821 ttcgagttag ggtgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    11881 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    11941 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    12001 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    12061 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggccgaa
    12121 gaatacagtg attcttcttg gaatgcagcc aatttgatta gcgaggattg cgccattttt
    12181 attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta
    12241 gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat
    12301 atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcaac tttctcctct
    12361 caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt
    12421 gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa
    12481 gaatatcgga aaacttaaga aaagatcctc atttagtaat gaggaagata gtgtttgata
    12541 ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cttcagcctg
    12601 tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt
    12661 ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg
    12721 cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt
    12781 gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga
    12841 ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat
    12901 cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc
    12961 cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact
    13021 ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag
    13081 gataagacgt tccttccaat aatacggaag acgcacgctg ccatttccaa tcgaaaaaaa
    13141 gaagatcaaa gaaattatgg tcaagaataa ttgtcagttg aatttgtttc aggaagaaag
    13201 cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga gagaatccca
    13261 aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa
    13321 aaaagacttt ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa
    13381 agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga
    13441 aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatcct ctgattgcaa
    13501 agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat
    13561 tttcccatac gatacgactc atttgacgat ctgtgcaact tactttgcac agcagatgca
    13621 acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct
    13681 actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa
    13741 tcctgaaatt caccattata cttctgataa aaaaatactg atcatcagtg acttaagtct
    13801 tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca
    13861 ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga
    13921 tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga
    13981 tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa
    14041 aaaacagcta tgtataaacc accctgataa aaatttcagg acaaacacac atagctgttt
    14101 ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact
    14161 cttcttttgt ccaattattt gcaatgagat ccgtcaatgt gttcattacg agggacatgg
    14221 aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta
    14281 ataattccag ccattttttg ttcattggag aatcagccga tagttctcta cgtttggttg
    14341 tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat
    14401 ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt
    14461 cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca
    14521 aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccctatct
    14581 tgtcaacgat ctctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta
    14641 ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca
    14701 tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt
    14761 cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca
    14821 ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg tttctcatga
    14881 gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt
    14941 ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc
    15001 tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa
    15061 gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcgact
    15121 agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat
    15181 ccaatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagagagtga
    15241 ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa
    15301 gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa
    15361 aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact
    15421 tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta
    15481 ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcaacaatg atcaaaatcc
    15541 cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca
    15601 gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg
    15661 actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc
    15721 caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa
    15781 ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag
    15841 gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt
    15901 tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag
    15961 caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg
    16021 gagaaactgt gtaataagtt tcaggaaaga taccaacaat cgtcaaagga tcgtcgtaac
    16081 tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga
    16141 tgatttcatt ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt
    16201 ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt
    16261 acaatccgat tggtgctttc tctccttctt caatgtattt cagatctcct cttgttaatt
    16321 gtgaagctgc aaatacttga tttgcatcct ccgataaaac aatctcttgt gcgtcccaat
    16381 catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata
    16441 cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc
    16501 gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca
    16561 aaatgtcact cgttgaatag tgtaactcca aagtgacatt ttgtcactta tgttgttttt
    16621 tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa
    16681 aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg
    16741 tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa
    16801 agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt
    16861 cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa
    16921 tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt
    16981 gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta
    17041 tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg
    17101 ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa
    17161 aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt
    17221 gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg
    17281 gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt
    17341 acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg
    17401 ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc
    17461 gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta
    17521 ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa
    17581 attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt
    17641 taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat
    17701 caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata
    17761 aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc
    17821 tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc
    17881 gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag
    17941 aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta
    18001 tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc
    18061 gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat
    18121 tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc
    18181 atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca
    18241 ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt
    18301 caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga
    18361 tggccaccat agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa
    18421 ttgacgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata
    18481 cgaatccaga agataagatc attttggact catctgttaa ccataaagaa tggctgattc
    18541 acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat
    18601 tttatcggga agatcaatct agggcgaaag agactggcgg atacgggctt ggcttggcaa
    18661 ttgcaaaaca ggtaatcgaa cagcatcatg gacaaatttc tgtatccaat tatcttccac
    18721 aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa
    18781 aaaagggctg tgacaaatgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg
    18841 aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat cgttcatgat
    18901 tacttggatg gtaaaggcgg aagtgattat ctggcaaaga aatattccat taaggctcca
    18961 tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg tctagtaaga
    19021 aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga gttatatcta
    19081 acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa ccctaatttg
    19141 atcgctagtt ggatgcgtca attccgtgag ggtgggatcg attgactctc aaaaacgaaa
    19201 ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa atctacaact
    19261 aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt ccgttccctt
    19321 caaattgaaa atgccttttt aaaagaattg aggaagctgc gaaaacagga agcccaacaa
    19381 cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt aaattagttg
    19441 agcttctaga aactttaaag ttccctaagg caacctttat gtactggcaa aaacgattgg
    19501 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaagttcgg gagaagcaca
    19561 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
    19621 aaaagaaagt tcagcgcctg atcaagaagc taggaataaa agtaacgaca tacacacgta
    19681 aatcacggcg ctacaattct tatcgaggac aagtcggagc agtagccaaa aatcggatcc
    19741 atcgtcgttt ctacaccagt atctgccatc agaaaatcac gacggataca actgaattta
    19801 aataccatga agtggatgcc aaaggcatta tccgtcaaaa gaaattgtat ctagatcctt
    19861 ttatgg
//
LOCUS       DANKHK010000043        19753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000043 DANKHK010000000
VERSION     DANKHK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(772..1725)
                     /locus_tag="PW109_001465"
     CDS             complement(772..1725)
                     /locus_tag="PW109_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2511298.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1748..2503)
                     /locus_tag="PW109_001466"
     CDS             complement(1748..2503)
                     /locus_tag="PW109_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511299.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2500..3459)
                     /locus_tag="PW109_001467"
     CDS             complement(2500..3459)
                     /locus_tag="PW109_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2511300.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3452..4402)
                     /locus_tag="PW109_001468"
     CDS             complement(3452..4402)
                     /locus_tag="PW109_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2511301.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4552..5004
                     /locus_tag="PW109_001469"
     CDS             4552..5004
                     /locus_tag="PW109_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2511302.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5093..7360)
                     /gene="gshAB"
                     /locus_tag="PW109_001470"
     CDS             complement(5093..7360)
                     /gene="gshAB"
                     /locus_tag="PW109_001470"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2511303.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7547..8545)
                     /gene="ftsY"
                     /locus_tag="PW109_001471"
     CDS             complement(7547..8545)
                     /gene="ftsY"
                     /locus_tag="PW109_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2511304.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8556..9365)
                     /locus_tag="PW109_001472"
     CDS             complement(8556..9365)
                     /locus_tag="PW109_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2511305.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9374..12955)
                     /gene="smc"
                     /locus_tag="PW109_001473"
     CDS             complement(9374..12955)
                     /gene="smc"
                     /locus_tag="PW109_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2511306.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12975..13661)
                     /locus_tag="PW109_001474"
     CDS             complement(12975..13661)
                     /locus_tag="PW109_001474"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2511307.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14009..15790)
                     /locus_tag="PW109_001475"
     CDS             complement(14009..15790)
                     /locus_tag="PW109_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2511308.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15816..16730)
                     /locus_tag="PW109_001476"
     CDS             complement(15816..16730)
                     /locus_tag="PW109_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511309.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16757..17719)
                     /locus_tag="PW109_001477"
     CDS             complement(16757..17719)
                     /locus_tag="PW109_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511310.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17719..18663)
                     /locus_tag="PW109_001478"
     CDS             complement(17719..18663)
                     /locus_tag="PW109_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511311.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18664..19668)
                     /locus_tag="PW109_001479"
     CDS             complement(18664..19668)
                     /locus_tag="PW109_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511312.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5462 a         4256 c         3518 g         6517 t
ORIGIN      
        1 agtataaaat caaagcagaa gagagttatt tctatcaatt attaaaacgg tggagtaagc
       61 ttcccgccga tttttatcga aaaatgcaga tcatcaacta tcaaccgctt gctgagacac
      121 atgcctaaat agaggttgtg aaatcagtgc tttgagctaa taccgaaggt cagctgattg
      181 aacattgttt agaagagatt gtgacaactg cgatttgctt tgagtaataa gaacgctgga
      241 cagaaaatag cttctcatat tttctagtct agtgggctta ttgccgagaa gcagcagatg
      301 gaacatcgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg
      361 acagaaaata gcttctcata ttttctagtc tagtggactt attgccgaga agcagcagat
      421 ggaacatcgt ttaaagaaga gatcggtaca aaataaaaat atctaaattt aagttttatc
      481 taagtttcgc tttgtacctt tgattcaaat caaaggaggc gatcttgatg ctgaaaaaat
      541 ggatcacact attttttgcc actccccatt ctagaatcaa cccgtatgat gtagataatg
      601 aattcacgtt gaaaaaagat ttctcaaata aataaaaacc atccgtaaaa tcatcaatac
      661 gattttacgg atggtttttg ataacttctt tgaggatagg ctcttgctat ttaagaatgt
      721 aagaaacatt attgttacat agtatgagag tttaatcctc tgttatttat gctatttaaa
      781 tgcttgattc acatcttcga tcatcagttt cattgattct aaaccgcccc cgcttaaata
      841 ccagacatct ggttcaaggg agatgatctg ctgattttta ccagcattcg tttgtgctac
      901 cagttcattg gcagaaatat catctttgct gtcgtctcca ccaatagctt ttgttctgtc
      961 tactacaaat aagatatccg gatttttttc cagcacatat tcataagaaa cactttgacc
     1021 gtgagtggat gcttcgattt gatcatctgc ttgttcaaag ccaaataaat catggatgaa
     1081 accgaatcta gaaccagatc cgtaggctga taattgccct tcattcacaa gtgtgactag
     1141 tgcttttttg tcagtagctt ctgctttctt tttcgtctga tcgattgcac tagataattc
     1201 ctctaattga gttttagctt cttcttgctt accaaagatc tcccctaaag cagtgacatt
     1261 ttgctggaaa gattcccatg gatttttcgc atctaaagaa aggaaaatgg tcggagcaat
     1321 ttgacttaga tctttttgaa aatctctttg tcggccagaa atgatgatta gatctggctg
     1381 aagctgatta attttttcta agtctggttc ttttattcca cctgctgatt ctactttttt
     1441 ataggatgaa agatactcag gaaggctgct tgtagctgct ccaactacct tatctccaac
     1501 accaagagca tccatcgtat ctaaagaacc attgtcgaat acgacgactt tttctggatt
     1561 atttggcacc tcgatttggt ctccattact atctgttatc gtcaatgtag tttcagtttg
     1621 tgtgctgctt ttttttgtgt ctgcctgact ggtgttgttg ctatttccac atgctgctaa
     1681 accaaatgat gctagaatca agatacctac tactaatttt ttcatctctt ctctcctaat
     1741 gttagtttta gaaataaaga cagaatctgc ggccttcaat ctcacaaatc cgtatgtcca
     1801 tttcgtataa ttcatccaat acatcttttt ttatcatttc ttgggtcgtt ccttgcgtaa
     1861 ataactgtcc atctttcatt gcaacaattt cgtctgcata acttgcagca aagttgatat
     1921 catgaagaac aataacaatt gttttcccga tttcttgtac taaatttttt aaaatctgca
     1981 tgatttgaac cgcaaaattc atatctagat tgttcaaagg ttcatccagt aaaatataat
     2041 ccgtgtcttg cgctagcacc attgcaatat acgcacgttg gagttgaccg cctgaaagag
     2101 tgtcaatcga ttgatccgct atttcttcta acccaaggtg ttccagtgat tcctgaacga
     2161 tttcctcgtc ttttgtcgtc aattttcctt tcgtataagg aaatcgtcca aattctacta
     2221 attctttcac ggtgatttgg acatttattt cattcgattg tcgtaggatc gataattttt
     2281 tggctaattc attggatttc caagccttta cttcgttatg gtctaaatag atttcacctg
     2341 tatcttttgg tatcaaacga ctgatcatcg acaacaccgt tgactttcca gcaccatttg
     2401 gaccgataaa agcagtcaac ttcttttcag gaatcgttaa ctgtacatct ttaaccactg
     2461 attttccatg gtacagttta ttgacctttt gtaaatcaat cataggatac tcttcctttc
     2521 gcgaatcaat tttgtgacaa aataaattcc tccgacaaat tcaacgacga cactcactgt
     2581 tgtattccag ccgaaaatct gctctactgc gagctgtcct cctgtcagaa acagcatagc
     2641 taccagaaag cttccgataa atagctgacc atgacgataa gtagaaaaga cacgataact
     2701 gatatttgcg acaataaacc ctaaaaaagt aactggaccc acaagagccg ttgatgtccc
     2761 tgtcaaaaga ctgacacaac ataaaatcaa aaattcaaaa cgatctgtgt ctatcccgag
     2821 gtttactgct tgttcatttc cgagatggaa aacgtctagc acatgtcgat atcgaaataa
     2881 caggtaccag cagccgataa tgatcaaaga agcaggcaaa agatactgac tctggatatt
     2941 gccaaagctt gcaaataatc tgccttgaag caaatcatat tcattcggat ccatgatgac
     3001 ttgtaaaaag gtactgacac taccaaaaag cgtacctaaa atcatccctg acatcagtaa
     3061 taaaaaaaga ttcgctctca tttttttcaa aagaaagaaa aataacaata gactcagccc
     3121 gaccatcagc ccaacgttca ataagaaaaa aactgtacct gtctgctgaa gcatcgtcac
     3181 tcctccgata aagaagaaaa ggaccgtttg gacaaggaca tataacgact caagtcccaa
     3241 tactgaagga gtcaaaaact ggttatgcgt aattgtctga aagctgattg tggcactgct
     3301 gacagctagc gagacaagga taaatgcaaa aattttctta ctcctcaatg cacatgcaaa
     3361 atgccagttt ccatatgtat ggatcgtcaa gtacattcct gtaacaaata aaacaagaac
     3421 aagcatacca ataaaaatag atggcttttt tttaagcata tcgtcgtccc cttatcagaa
     3481 gataaaggaa gatagcccca ccgatgattc caacgatcac actgactggt atttcataag
     3541 gaaaaatcaa cacgcgagcg acaagatcgc aaaatactaa gaacaaacta cctgctaaag
     3601 cagttacgcc aattgttttg tgcacttgat ctccatatat tctagagaca agattcggaa
     3661 tgatcactcc gataaaagga atgcttccta ccatgatcaa catgactgca ctagccaatg
     3721 atactaagcc aatgcctaaa aactgcatct tctggtaatc tattcccaga tttactgcaa
     3781 acgattcgcc cagaccaatg atcgtaaatc ggtaagcaaa tagccaaacc aagagcaaca
     3841 atggaacaac agcatacaat aactcataat ttcctcgtat cacagtcgca aaattccctt
     3901 gaagccatga agtcatattt tgtaccaact ggaattgata agcaaaaaaa gtagcaactg
     3961 aaccaatgat attccctaac atgactccaa tcaacggaag catcaattga tttttctgga
     4021 ggaacaatcg cgtaagggat aaaaaaagca atgttcctgc aaaagcaaag caaaatgctg
     4081 tcaaggatcg gacaatcaag gatgcaccag gtaggaaaat catagccact aaaataccta
     4141 aacgcgcact atccatcgtg cccgcagtcg ttggtgaaac aaatttattt tgggtcaaat
     4201 gttgcatgat caacccacaa atactactgg tcgctcccac aaggatcaaa caaatcgttc
     4261 gtggcaaacg agtcactagc aagatttgta agctttgccc atctaactgg agcagttctg
     4321 acaattgtac attcgatact ccgatgaaca atgaaatact acttaaaata actaataatg
     4381 ctactagtcg tccgtagcgc atccaatcca cctttctaat tgagaatgat tttcattctt
     4441 attttaagtg aatcgttgaa caaaagcaac cgctttttca tatgttttag gtattattgg
     4501 gaatatgcta taatcgtaag aaagacaaag agaagaagga gggtattttt tatggcacaa
     4561 gatgatgtaa aaagccattt ggataaggga ctttatggta cacctttagt gaaccctgaa
     4621 gaacagcata aatatttagg gacattcagg gaacgctgct atcttagtat gactgttgct
     4681 gaaatgatcc aaccaaaaaa caaagaaaac tttttaaaag aactcaaaaa acatccagat
     4741 gcttccatac tcttgaatgg ggctctaccc ctttctgtcc aaaaccaata tatccagcta
     4801 gcaacgaaaa acaatagccg tttcactgta gtcaacgatt acgtcactgc aaatcctgag
     4861 gaattcggat tgctcttaac agcaaaagaa gcagtaaatg aacctgtgat tgatatcgaa
     4921 aaaaaatacc cagaagaaaa ctcttcgcca gctgaaaaag aaaaaccaaa aaagaaaaaa
     4981 agcttctggg atcgtttatt ttaaaacgaa aaaacacata aatttttaca tgcaaaaaaa
     5041 gcttttgatc agattgacat caaataggac aatctgtcaa aagctttttc ttttaaaata
     5101 cttcaggata taacaatttc aatacattca tcgtcaatct tctgcctttt cctgcaaaag
     5161 gataaacatg catatgcatg gctggattga aatttgcttc gatgatccca tatgcttttt
     5221 tatcagttgt aggatcgatt tctttatctg gtataatcaa atcgatacca ctaatcttag
     5281 ctcctagtgc ttctaccgca gaaacggcga tttttttgta agcttcagaa aattcatcag
     5341 tcatatcgat cgaatcacca cctgtactga tatttgagtt ctcccttaga taaacaattt
     5401 gattggcttg gggaactgat tcgatcgtca acccttgttc tttcaacatc aattgttcta
     5461 attcacctaa ttggatcaat tctaaaggtg cacgatgatt cgttccacgc aaagggtcac
     5521 tatttttttc ttcaacaagt tctttcactg ttcggatact gtcaccaata acattagcag
     5581 ggactcgcaa catgatggct tggacttctc cgtcgatcac aaagaaacga tattccgttc
     5641 ccggcataaa ttcttctact aaaaccgaac tgtcttcacg aaaagcaatg gctaaacctg
     5701 cttgataatc ttccagacta gctccttcct tgaaaatcgt gatacctaac ccgtaattgg
     5761 ttgatttagg tttgatcaca aatgcctgct cggcaaatct cggataagct tttaccgctt
     5821 cttccatgga tgaaaattct gcgcctcctg gtacccggaa cccagcttct tttaagactt
     5881 ttttcgttac tgttttgttt tccatgataa gtgggacgat atagctgtcc ttacttgtca
     5941 tgtttgcatt tttcacatat tctacatggt cttggaattg cagacgcaaa aattgatccg
     6001 actcatctaa tactttgact tggacacctt tttggatggc atcgaacata aatatctgag
     6061 tagaaagttc catttcgcga aaaccggcca actgataggg acgttcatgg ctttctttat
     6121 aatattgatg tcccaatgcc acagcaactt cttgctgact cgatagctgt gcttcttgat
     6181 agagtttcgc tgctaacgtt tgttcaggat gatccatttg agtcaatgct tcctctacta
     6241 aataaagaga atccaaccaa tccaattcaa ccagcatttg cttcattcct tcaagaattt
     6301 ctctaccttc ttgatgaaat tctgtttgat ctgatggttg ttctagtgct actttgttat
     6361 ttcgagtcgt accttctgct acccattggt cacagtcttc tttttcttct gtccataaca
     6421 tgtacatcag gaataattgt aagaaccgaa cttgctctgg attgataccc aatcttgcat
     6481 aaggattcaa atcgatattc cgcaattcaa tataacgaac acctgttgtt gctaaatctg
     6541 cgactttctt tccgcctcgg aaacgaagcg gtgtataaaa ttctttctcc tccgaaagtt
     6601 tcccttcttc gatcattcgc tcgatatctg ctaaatactg tttcatagaa gcataggaaa
     6661 ctttcacatt tggatgattc gtatagccca gcgcactgtt gcgaatactg cgaacaggtt
     6721 cttgcggatg tgattcatct aaaaagtagt tcttctcact cattggtgaa gcaccgaata
     6781 aataagtgat catccagcga tagcgcataa agtttctcgc tgttttcaaa taaagttctg
     6841 ttttgaattc ggaaaaatct tgaaattctt cctgatggct gaacaatgtt cttagcaact
     6901 catcgccaaa ttcaaaattg aaatgaatac cgcttaccat ctgttttcgc ttcccgtatt
     6961 cttttgctaa ataacgtcga tacaagacat cttcgaaatt ttttaatttt gcaataatga
     7021 tctcttcgtc tttttccggt aaggcaggtg gcatacttaa tggccagatc atctcttctt
     7081 tcggtataga acgagcagtc acatcataaa cagcagcgag atactgaaat aattcgggaa
     7141 tagaatcggt aacaggggtg atcatctctg tttgtgtttc gctaaaatct gtttggatat
     7201 aggggtgata ggatcgatcg ccaaagattg ctggatgatc ggtttttgct agatttcctg
     7261 caagatcaac ccgctgtcct tcccgttcta taccaaatcg agcttggtcg ataaaagggc
     7321 gcgcattgac atgcaataat aattgcttaa aattcatcat taagtgacct ccaattcata
     7381 tgaatctaat tataatcaaa aagtctaaga aaaagaacga aattgtttga gaaccgctta
     7441 caaataagta taaaaatgaa ggcatcaaaa acacagctat gttaaaaaca atcattcaaa
     7501 caaggagctg caacttttgt cacagctcct gctttttaga catgggttat tcttctttta
     7561 gtaatccttt gaacaatcca acgacaaaat catttggatc aaacggctcc aagtcatcga
     7621 ttccttcacc aagaccgacc aatttcactg gtaaatgcaa ttcattacgg atagcaagta
     7681 cgataccgcc ctttgctgta ccatctaatt ttgtcaaaac aagaccggtc acatctgttg
     7741 tttctttgaa ttgttttgct tgaaccatcg cgttttgacc agtggttgca tctaatacaa
     7801 gaagtgtttc ttggggtgct tctggtaatt cccgctggat tactcgtttt attttttcta
     7861 attcgttcat caagttcact ttattttgca gtcggcctgc agtatcaact agtaaaacat
     7921 ctgcctgctc tgtctttgct ctttccaaag catcgaatac gacagctgct ggatcgcctc
     7981 ctgcattgcc acgaacaact tcaacaccag cgcgttcgcc ccagacaact aactggtcga
     8041 ttgctccagc gcggaaggta tctgctgctg ccatcaatac ttttttacct tcgtttttga
     8101 actgatgggc aagtttacca atacttgttg ttttccctac accattaaca ccgacaaaca
     8161 aaataactgt tagtccgtct tcttggatat ttagttcatt tacttcattg attccttccg
     8221 cttcatagag atccacaagt ttctcgatga tcgcattttg gacttctgcc gattttttcg
     8281 catttctgag tttcacttct tgacgtaatg cttcggtgat tcgtatcgct gtttcaaaac
     8341 ctacatctgc accgataagt gtttcttcta attcttcaaa aaagtcttca tcgactgttc
     8401 gaaaattggc aaacaactca ttcatccgtt caccaaatgt tttacgcgtc ttttctaaac
     8461 ccttgtcgta cttctcctgt acttcaactt cttctgtttt ctcttcgcca gtaaaagcac
     8521 gcttgatttt atcaaaaaat cccatttgac tccccctact ctaatacaaa tttttggatc
     8581 actttggcta cgccattttc ttcattgctg gctgtcacga aatctgctgc ttctttgacg
     8641 aaaggcacag cattctccat cgctacgcct agtccagcat attggatcat cggtaaatca
     8701 ttttcctcat caccgatcgc catgatttct tcttgtttca aaccaagatc ttttgccaat
     8761 aaagaaatac cataagcttt tgtaacacct tttggcataa attccaaaag catacttctt
     8821 gttttgatga tctcatacat ttcatggaat tctgctggta tttctttgat tttaggatct
     8881 aattcttctt ggtcataagc gattactgcc ttattaagca caaattcatc tgtaatatcg
     8941 cttatatccg ctggttggaa ttgcagcagc ttattcaaca cattgtacaa ggattgcttc
     9001 cttggggcac taggcagctg taaaaccaca ttgtcagaca aaacatctag tggcagatcc
     9061 aattgctgag ccagtttgta taagcgttgg atatctgata cttccatcac tttcttttcc
     9121 atgatttcac cagtatcatt tttttgtacc aaaccgccgt tgaaagtgat gctgtagtca
     9181 ccttcatcta ccagtcctaa ttcttgcaga taatgcgcca tcgcagctaa ggggcgtcct
     9241 gtacatagaa cgatcttgac cccttgctgt tttgcttgtg ctaatgcttg tttattctca
     9301 tctgaaatca gcttttgttc atttaataat gtaccgtcca aatcgattgc tactaatttg
     9361 atcattttcg ttcctacact ttcgtttcgt tgattttcgc ctcttcattg atatcttcca
     9421 tccgtacaga aacgattttt gatacaccgg attcctgcat cgtcactccg tacagtacat
     9481 ctgctgcgac catcgttcct ttgcggtgtg tgactacgat aaattgtgtg tcatcttgga
     9541 aatcgctcaa gtaacgacca aaacgtttca cgttggcttc atctaacgct gcttctacct
     9601 catctaagat acaaaacgga acaggacaga cacggataat ggaaaagagc aacgcgatgg
     9661 ctgtaagtgc tcgttctcct ccagaaagca aacttagact ttgcagtttt ttgccaggag
     9721 gttgtacttc aatctcaata cctgttttca gcaaatcagt tggttccgtc agtactaatt
     9781 cagctttacc gccgccaaac atatttggaa agacaatttt gaattcttga cggatagctt
     9841 caaatacttc tttgaatctt gtccgcactt catcatccat ctcatccatc gtttcaaaca
     9901 attggttttt cgctgctaac agatcatctc gctgtgttgc taagaatgta tgtctttcac
     9961 tgacttgttc gtattgttcg attgaattta agtttactgg tcccagtttt tcaatctgtt
    10021 cttttaagga tgaaaccttt gttctactgt cttcgatatc ggttgtttct tgatagtctt
    10081 gggaagcttt ctcaaatgtc agctgatatt ctgactgcag ataggtcaaa tgattatcta
    10141 aaatcatttc agctcggtct ttttggactt ctaatttgga ttgttccgac agtcgtgctt
    10201 tttgctcacg attacgctct gccaagactg cttcaagctt atcgatctcc ttttgccttc
    10261 tatctcgctg ctctttggct tttgccaact cggcttttaa tgtttctcgc tgagcagata
    10321 attcgttaat ttgtttttcc aaactttctt ctgtgacttc atgatcagaa aagtctgctg
    10381 tcaatgtcgc caactgcttt tctatcgctt cttgacgttc ggttgcctct gctttttgga
    10441 ctcgggcacc gcggagctgg atctgcagat gattaaactg ctctttcaag acagcaagat
    10501 ctgcttgttc ttgtgctttt tgactttgta cttgtgctct tctggcttcc atctggtcgc
    10561 tttcttgact taacgatttg atttcttcgt cgattttttg tcgttgcgat tcaagatctg
    10621 tttgtttgtc tttcaattcg ttcgtttgct tttcgtaatc ttcgataaac tgctgcagtt
    10681 cacgtgtctc aaaagttgag atttgctttt ctttttcaaa acgttcaagt tcatttatga
    10741 tgttttgtag ctgatttgtc gcttcttgtt cttcaaaacg taattgttcg cctcgtgtac
    10801 gcaaaacttc ttgttcttcg gctaaacgag ctgtttcttg ttgcagctcc tgaacttttt
    10861 tttcttgtgc ttggagttgt ttatctgctt cttcaaattc actagtcaac tgtttcaatt
    10921 cttgattttg tacaaacaaa ctgccttggt ttcctcgttt attcgctcca cctgtcatgg
    10981 atcctccggc attcatcaca tcaccttcta aggaaaccac tcgatattga tagcggatcg
    11041 tttgcgcgat tgcattggca ctagttaagt ctttggctaa aaggatcgtt cctagcaggt
    11101 tttgaaccac agtttggatt tgatcgggat aactgacttg ttcactggca atccctataa
    11161 acccttctac agctgctgct tgtgtcaata tatgagccgg taattgtctt ggtttgattg
    11221 tcgtcagtgg aaggaatgtt gctcttcctc ctctttgctg tttcaaatac gtgatggctt
    11281 gccgagcatc tttttcattt tcaacgatga catgctgggc tgccccacct aaagctgttt
    11341 cgatagctaa cgtgaaatct gcaggaacat cgatcagttc agccactgct ccgacaatac
    11401 ccgaaagctg ctgtttatgc tgcaagacca atcgaacccc ttggtaaaaa ccaaaatagt
    11461 tttcttggat ttcttgaagg cttttcttac gagcacgtag ctgctggact tgattcatta
    11521 attgatacat tttaggctgt tcttttgcta atgcttcttg gaactttttc ccgttttgtt
    11581 ggatcatttc taacttctct tttgtttcag ttaaagatga ttttagctgc gcctgctttt
    11641 gtgtcaattc ttctttttgt aagatcaatc ggtctactga tgcttctact tccgattgct
    11701 tggctaacgt ctgcttgctt tttgctgttt cttgtatata ttgacgttct agatacttca
    11761 gttcgtttcc tacagctgct tgttcttgca tcaaatcaac atattgatct cttaattctg
    11821 ccaataattc tttcgttgat ttttggtatt tttctaattc ttgctgtgtt tttatgagat
    11881 ttgcttcagc tttttggatc tctgtttctt tttctgctgc tgctttcatc aagctttctt
    11941 gaagcttttc aaaatgttta accttttttt gtgcttctgc taatgaagtt tgatattctt
    12001 gcgagctttt ttgtgtatgc tttgtccgct cctgcaaaac atctttttgt ccttctgtct
    12061 gcttcagttt ttccgaaaga tctaataata cttgctgatt tttttcgatc agacgatcgg
    12121 cttgcgcatt ttctttacgc tgcttcgcca aaatggattc ttgttcttgg atcgactcag
    12181 atagtttgcc taattctaga ttgaatttcg caagctgtgc ttgtttgttg tcccaatctt
    12241 tttttgctgt cttgatttca gcaaccatca aagagacatc ggtttgagtc agtgtttctt
    12301 ttagccgtaa aaactccttt gctgcctcac tctgggcggc caaaggagtt aactgttctt
    12361 ccaattcgtg gatgatgtct tggacgcggc tgaggttgtc ttctgtttca aaaagcttct
    12421 gttctgcttt ctttttccgc tgcttgtatt tcaagacacc agctgcttct tcaaagatcc
    12481 cacgacggtc ttccggttta ctactgaaaa tcgcttctac ttttccttga gaaatgattg
    12541 agaacgattc ttttcctaat ccagaatcta agaacaattc ttggatatct tttaatctgc
    12601 agctttgttt attgatgaaa aactcgcttt ccccagtccg tcgataacgg cgggttacac
    12661 tgatctcatt gtactccaat ggcagataat gatcactatt gtctaaaatc actgtgactt
    12721 ctgcaatatt cagctgctta cgggtatcgg aaccggcaaa gatgatatcc ggcatttttc
    12781 cgcctcgcag acttttagca gattgttctc ctaataccca gcggatagcc tctgtaatat
    12841 tgctttttcc gctgccattt ggtccaacca ctgctgtgac gctattttca aaatcaatga
    12901 ctgtcttgtc tgcaaatgat ttaaaaccgg ctatttcaat tcgtttcaaa tacaaagctt
    12961 tccctcactt acttttacag tgctgccaat gcattttccg ccgcagcttg ttctgctaat
    13021 tttttcgatt tcccttgtcc agtgccaatc aattgatcat ctacataaac ttcgatccaa
    13081 aatactcgtt catgagctgg accttcttca ttgatcaatc ggtattcgat tgatacatca
    13141 cctgattttt gcaagacttc ttgtagtttt gttttatgat ccatctcatg tgaaaaagca
    13201 cctgctttga ctttaggaaa aatcactttt tcgatgaatg catgggtcgt gtcgaatccc
    13261 tggtcaagat ataacgcgcc taaaaaagct tcgaataggt cgcaaagcaa tgctggtcgc
    13321 gtacgtcctc ctgaattctc ttctcccttg cccaacaaaa catattgatc aaagtgacat
    13381 tctttggcaa atttcgacaa gctgtcttcg cgaacgatcg cagctcgtgt ttttgtcaat
    13441 tttccttctg gcaaatgagg atataaacgg aataaatact gagaaaccat cagttctaaa
    13501 acggcatctc ctaaaaattc aagtcgctca ttatccgata attgcagatt gcgatgctca
    13561 ttcacatagg atgaatgggt aaaagcttgt tcaagcaaat ttagatcgtg aaaaacgatg
    13621 ttatagtttt cttttagttc ttttgtcagc tggttgtcca ttttccacat tccttttatg
    13681 gatatttttt cgatacttcc ttattatact ggttttttgt caaatttgaa atggttcggt
    13741 caaaaggtaa gtaaagcttt aataatccta gtgattgacg agaagttgtg aaaaattcgt
    13801 ttagtttcga gtatgaagaa aaaattttgt gaaaatggct ttccatattt ttgcataaat
    13861 ttgacttaat accgagaaac tggactttga acaccgttta ttggagaagt catgaaatca
    13921 gcgccttgac ctgaatatta gagaaataaa cagaaaaata tcaaaaaacc gaaaaaggac
    13981 atcgcccttt ttcggtcatt tttagtgatt atgaacgtga atctgctgta acagaaacat
    14041 ttgcccatgg gtttggtaca tcataagacc aatcccagtc tttgacacgg tcgttgactg
    14101 gcaatacttc attacggaac aacgttggaa tcgcaaatgc ttcttcaaat gcatattgtt
    14161 gccatttttg atatgcttct ttttggtact cttcatcaaa tgctttgctt gaactgatat
    14221 cgttgatcaa ttttgtattt tcttctgatt cataacgagt gtagttgaac gcagcatttg
    14281 gtccccatag tccacttgga tttggatcag ttcctgtatt ccaagcagct tggtaaacat
    14341 caatttcagg atcatcgttt tctaatttat catagaaaga attgaattcg atcagacgac
    14401 ctgttgtgta ttggacatcc aaaccaattt ctttccattg ttggatatag taatctgcca
    14461 atggttgcgc tgtttcccca ccggacatcg aagcaaagtt gattgttagt ttatttccat
    14521 ctttgtcttc tcttaaacca tcgtcatcca catctttgta gccagcatca tctaatagtt
    14581 ttttcgcttt gtctagatct tgtgtgaatc ctttcatctc atcatcatgg tatgactgga
    14641 atactggagg aatcaatgaa gtcgcattgc ttctcaaacc tgcatagaaa cgttcaccta
    14701 ctgcattatt atctaatgca tagcccattg cttgacgtaa tgatttatca gccatttttg
    14761 aatctggatt gtattccaca ctctttgtat ctgcattcca tttacctaac ttgaatccta
    14821 gataagtata agaagtttgt tcacggccta agtttgtata gccagcaatt tctttatagg
    14881 tatcgtaagt atctgttggc atttggaaaa tcatatcata taatttagag ttcaatgctt
    14941 cagttgcact tgccatcggt acagatttca tcgtgatctt tttcaattta ggcgtaccat
    15001 tccagtagtg ctcgtttggc aagtattcta ctgattctcc tgcaacgata ttactcatgt
    15061 aataagggcc aaaagtcact ggatttttac gtacttgatc gcttgattcc atatctttga
    15121 ttgctacacc ttctaacgta tgttttggtg ctgcatatga ccagatacca ccacccgatt
    15181 gaagcatgct tgggtttact tctttatatt cgatttcaac tgttttgtcg tctatttttt
    15241 tgattccgga gattgtgtcg gcacttccat catgatattc ttccatacct acgatgttac
    15301 ggaaagtgtc gtcataacgg ataccagtgt aatctttatt cccaatgatt tcgtaaggga
    15361 aaatcacatc gtccgccgtt acatcttcac catctgacca tttgacgttg tcttttactt
    15421 tgattgtcgc ttttttcgta tcttcatcta gatcaagcgt tgcagctccg tcgtctgtga
    15481 ttctgaaatc gtcatcgttt gcaaatagcg gttcatgaga aggagccata aaagctgcat
    15541 catatgaatc ttggtaaaac tcccactgga acaatccttt aaactgactg tccgttgcga
    15601 ccgcagcttc taattcgccg tcttcgattg catcatcatt attttttgtt gcaattggaa
    15661 aagttaattc ctctttgtta ttgccgttgc ctccagagtt gtttccagag gtatctgtac
    15721 ctccgccccc gcatgctgca agaacgacag cagacatcag cgctgcaaat ccaaaaatcg
    15781 ttttcttcat tcctatttcc cccttcgctt ctatactagc ctaaccgttg gcgcgcatct
    15841 gcagaacgct tgaatgcttg tccaacataa ttgatactca acatcaaaac taaaataagg
    15901 attgatgcgg gtaaccagat ccattgccga ttcactaaga cttctccgtt acttgcataa
    15961 ccgatcaatg tacctaaact aggtacattt gccggcaagc cgaaacctaa gaaagtcaaa
    16021 gttgtctcga taccgatgtt tgccgcaaag ttcatcgtca aattagtaat gattaatgaa
    16081 cttagattcg gcatgatttc gcggaacatg attttaaaat cattggttcc cattgttttt
    16141 gaggcactga cataatctct tcttgcttca gataatgtct tgcttcggaa taatcgggct
    16201 ttagctaccc aataaaatgc actcatgatg aatataaagg accagacatt gtatctggaa
    16261 ataatcgata cgaagacgat aatgatcagc atgatcggta gaatcatgat aaaatcaacg
    16321 atccgcatca agatgttgtc aatgatccct ccgtagtagc cagagatgat ccctaatcca
    16381 accccgatga tcgaagtaag gatcgtgatc gcaaatccga tcgtaacaga gtttctagca
    16441 cctatgatca attgtcctaa tacatcacgt ccgccttcat ctgctcccag aatgaacttt
    16501 tgtccttgtg tcgaaactgt tcctggtgca gcgtatttgt cgaagatact aacatacatc
    16561 actttgtctt gatcggtcag catcgcaccg ataaagatca ctaataataa aaccactagc
    16621 aaacctaatg aaaaaatagc aattttatct tttttaaatt ctcgagcaat cattcgtatc
    16681 cccataggag gaatactttc ttgaactgct gtattgtttt tttcactcat cgatccattc
    16741 tcctttctga ttggttttat tgaatccgaa tacgcgggtc aacgatactc atgatgatat
    16801 ctgaaagtaa tgtccccact agcgtcgcga ttcctaaaat caatactaag gatgtgatca
    16861 cggtatagtc ccgttggccg atggaatcaa taaatagttt tccgactccc ggataaccaa
    16921 aaatcttttc gataactaca gaaccagcaa tcaacgaagt gatttcatac cccatttgag
    16981 aagccatcgg caacgctgca tttcggaaaa tatgtcgact gaatacttta ttcgtcggta
    17041 cccctttgga acgggctgtt ctgacaaaat cgagcgattt cgaatcgatt acttcacttc
    17101 gcagatattg gattgtgacg gcagtcgcca aaaatgcttg ggtaattgat ggtaacagaa
    17161 tatatttcaa acggtctatc cattgagtga taaatccacc ttgcaaacct agtgtttgtg
    17221 atccacttgt tgggaaccag cctaaacgat aaccaaaaac aaatagcatg atcaagccga
    17281 agataaataa aggaacagca aaactaaaga agttgtagat tacgacaatc ttatccaaga
    17341 ttgaattttg ataccggcca gcaagcatcc ccagaggcac agcaatcaaa taagtaagga
    17401 caactgtcaa gatcgataga tataaagtgt tgatgatccg ttcgccgatc aagtcagcaa
    17461 cagagcgttt gtataagaaa ctttgaccaa aatcgccttg caaagcattt ccgatccaac
    17521 ggaagtactg gacataccat gggtcattca agccagctgc ttgtcgcatc tgttcgatca
    17581 ctgccggatc ctgatttgga ttgatcaaac cggtaaatgg atcccccggc atcgcttggg
    17641 ctaataagaa gatcagaaca cttaaaataa taacttgcgg gatcatcaat aaaacacgtc
    17701 gcagaatcgt tttccacatt agcttgcacc tccatctttc aacgccactt tgtgtgtagt
    17761 tgtgatcgta cgcaaatcgt agactcgacc agtcgcatca tagtattctt tttggttttg
    17821 gatatactct tgttctactc ttcgtcgatt ttctttgtgg ctttccctat tttctacatc
    17881 gatttgcgga atagctgaaa gcagacgttt cgtatagata tgtcgcgggt cattataaat
    17941 gtcttctctt gtaccgattt ctacaaatcg acctttgtac ataattgcga tattgtcgca
    18001 catgtgtttt actacaccta aatcatgtga aatgaataaa tagctgaggc cgtattcttg
    18061 ctggattcgt ttcatgaaat tcaatacttg cgcttgaaca gatagatcca gagcagatac
    18121 aggttcatcg gctacgatca gttttggatt ggtagcaaca gcacgggcta ctcctagacg
    18181 ttgacgttgt ccgcctgaaa attcatgagg atatttatat agcgcatctt ccggcatccc
    18241 aacgatatct agcagtcctt ttaccttttt cttttcttct tgatcgctta accgttcaaa
    18301 atttcggatg ggttcagcaa tgatatccaa cactcgcttt ttaggattca aactggacat
    18361 tgaatcttgg aagatcatct gcacatcctt gttataaccc atttttttgc ggttgcttcg
    18421 cttcgttaca tcttttcctt catacatgat ttgtccggct gttacctttt ctaatcccac
    18481 aacggctttt cccgtagtag attttccaga gccagactct cccactaaac catatgtctt
    18541 tcctttttcg ataataaaat ccacaccatc tacagcatat acatgatccg ttactcgatt
    18601 gaagaatcca ctgcgaatag ggtaatgaac tttcaggtct ttgattgtga ttaattctgt
    18661 catctacacc tctccttttt cgtctctaaa gtggaaatgc ttatagcagg agcaacgtac
    18721 caagtggttc ggtgcgactt catgaaggat aggctcttct tcatgatcgc ttgctggtat
    18781 ccatggaata cgtggtgcaa accgacaacc tttacgaggc atgtttttca gagaaggaac
    18841 gacaccttcg atcacatgga gttctgcatc atgatttcct tcttgtggta tagagttcag
    18901 taaagagcgt gtataaggat gcttaggatg atcaaacagt tcttttacac ctgctacttc
    18961 aacgaactgt ccgccataca tcactgccac tcgatcagcc atttctgcta cgactcctaa
    19021 gtcatgagtg atcaagatga tccctgtttt tgtttcttcc tgaaggtctt tcagtaaatc
    19081 aagaatctgt gcttggatgg ttacatctag ggctgtcgtc ggttcatctg caatgacgat
    19141 tggaggtttg caggcgattg cgattgcgat gatgacacgt tgtctcatac cgcctgatag
    19201 ttcatgggga tattggcgtc caactctcgc aggattagga atccctactt gttctagcaa
    19261 ttctagtgca cgagcttgac gctgttctgc attcatcttt gtgtgatagg aaagtccttc
    19321 tttgatctgg tcttcgattc tcatgagagg atttaatgca gacaacggat cttggaaaat
    19381 cattccgata tcatttccgc ggattttatt atagagcgtt tcgttcaggt cgattaaatt
    19441 cagatcgtta tataaaatct cacctgtaat ttttgtgtta ttaggatcaa gtaatcccat
    19501 aatcgtagta gccaaggtac ttttcccgca cccagattcc ccaacgattg ctaaaatctc
    19561 gtttttgtct aaagtaaatg aaacgtcatc tactgcatcg taatagtcat ctttgagtcg
    19621 gaatcctgta tgtaggttct gaacttctaa taattggtta tcggacaatc gaatacccct
    19681 ctctgcttcc taaatggaag tttttcttaa taaaaaagga cattacacag aataaatcgt
    19741 tttataacaa agg
//
LOCUS       DANKHK010000044        19727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000044 DANKHK010000000
VERSION     DANKHK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(39..404)
                     /locus_tag="PW109_001480"
     CDS             complement(39..404)
                     /locus_tag="PW109_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511313.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            612..1094
                     /locus_tag="PW109_001481"
     CDS             612..1094
                     /locus_tag="PW109_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511314.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1291..2181
                     /locus_tag="PW109_001482"
     CDS             1291..2181
                     /locus_tag="PW109_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2511315.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2604..2840
                     /locus_tag="PW109_001483"
     CDS             2604..2840
                     /locus_tag="PW109_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511316.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            2847..4799
                     /locus_tag="PW109_001484"
     CDS             2847..4799
                     /locus_tag="PW109_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2511317.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4871..5368)
                     /gene="dfrG"
                     /locus_tag="PW109_001485"
     CDS             complement(4871..5368)
                     /gene="dfrG"
                     /locus_tag="PW109_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2511318.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            5791..8265
                     /locus_tag="PW109_001486"
     CDS             5791..8265
                     /locus_tag="PW109_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2511319.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(8368..10104)
                     /locus_tag="PW109_001487"
     CDS             complement(8368..10104)
                     /locus_tag="PW109_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2511320.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10101..10805)
                     /locus_tag="PW109_001488"
     CDS             complement(10101..10805)
                     /locus_tag="PW109_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511321.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10936..11439
                     /locus_tag="PW109_001489"
     CDS             10936..11439
                     /locus_tag="PW109_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2511322.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11399..11737
                     /locus_tag="PW109_001490"
     CDS             11399..11737
                     /locus_tag="PW109_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2511323.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11758..13176
                     /gene="ffh"
                     /locus_tag="PW109_001491"
     CDS             11758..13176
                     /gene="ffh"
                     /locus_tag="PW109_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2511324.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13288..13866
                     /locus_tag="PW109_001492"
     CDS             13288..13866
                     /locus_tag="PW109_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2511325.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13870..14391
                     /locus_tag="PW109_001493"
     CDS             13870..14391
                     /locus_tag="PW109_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2511326.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14470..15024)
                     /locus_tag="PW109_001494"
     CDS             complement(14470..15024)
                     /locus_tag="PW109_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2511327.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15277..15552
                     /gene="rpsP"
                     /locus_tag="PW109_001495"
     CDS             15277..15552
                     /gene="rpsP"
                     /locus_tag="PW109_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2511328.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15564..15815
                     /locus_tag="PW109_001496"
     CDS             15564..15815
                     /locus_tag="PW109_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2511329.1"
                     /translation="MKDLSDLVLTIIRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15940..16731
                     /locus_tag="PW109_001497"
     CDS             15940..16731
                     /locus_tag="PW109_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511330.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16901..17422
                     /gene="rimM"
                     /locus_tag="PW109_001498"
     CDS             16901..17422
                     /gene="rimM"
                     /locus_tag="PW109_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2511331.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVGLTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17412..18173
                     /gene="trmD"
                     /locus_tag="PW109_001499"
     CDS             17412..18173
                     /gene="trmD"
                     /locus_tag="PW109_001499"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2511332.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18309..18656
                     /gene="rplS"
                     /locus_tag="PW109_001500"
     CDS             18309..18656
                     /gene="rplS"
                     /locus_tag="PW109_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2511333.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18914..19207
                     /locus_tag="PW109_001501"
     CDS             18914..19207
                     /locus_tag="PW109_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2511334.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         6661 a         3480 c         3933 g         5653 t
ORIGIN      
        1 aagaaagtag cttctctttg tacatatatg gtttgttttt atggatgccg agctttttta
       61 taagataagg ctaccgccca taacgaccca aggatttgtg gaactaagta aatacctgca
      121 agaagcaaga tcagctgggt cggttttaaa ggatgataga agtaacgcca ttcagtaaaa
      181 ttggcgcaaa ataaatagga agtgacagta gatataatat tggcaatgaa taaagatata
      241 atacgcctgt tcctttgaaa ataatatcca gcaaaaagag ataaaaatat caatgtgatg
      301 aacccgctta cggaatgata acgaaaatca agcagtaagg cgaaatgcac ataaggaaca
      361 aaagataaaa gcaacggaag gtttttccag caagttttta tcatctttca tcaccaccta
      421 acaagtgtta ttattttgtt agttttattg taaattgaat cctgcaataa tcaattactt
      481 tttcattatt tctagattaa atgaataaac tctcatgcca ttatctctaa aaaaaaagtt
      541 tttcccccta atttttttag atagaaaatt gtatagtatg atgagagaaa aagtagtttg
      601 ggggaacgtt aatgagaaga aaagcagtaa tcgatcggct ttatttattt ttttggtttt
      661 ttcctatcgt tttgctacct tttacatggg taaatgctgt tgtacaaggt gcggagatat
      721 taggcctgat ccaaggaagc ggatttgtgg tttctaggaa ttttccagct ttgactgttt
      781 tctatttagt gataagtgta ggtctcttat taccaaaatc tctgatcaat cactacaatt
      841 ggcagctgct ttacttttct tgcttattag tatttacatc tatgttttct ttgctgcctc
      901 gcttgattat cggccctaat ggtttgagcg agagtggatt gaaggattca gcttattcct
      961 ccatgtattt cttgacgatc aaaccatggt tctatttagt aattgcctca tttattcttg
     1021 caacattttt ttacttctgg acagaaatca ggataaaaag tgaacgatct gtatctataa
     1081 aaaataacta ttgatggtgg atcatcagta aaaaagcata tgagctgcgt aaaatgcatg
     1141 tttctcatat gcttttttta ttattatgta aagaatgcga aaaacgttta catgattttt
     1201 acataactta actatatttt tacagcaacc atacgaggga tttacagtcg actgataaac
     1261 tttagttgta aataagaaag gagcaataaa atgaaaaaag tattgtttac tctaggtatg
     1321 atgggaattc ttttagccgg atgcggctca ggatcaggaa atacagatgg ctctgccaca
     1381 caaaataatt caagtgactc aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca
     1441 ttacagccgt tagtcgatgc agcacaagaa agctttgtac aagaaaatcc aaattatcaa
     1501 atctctgtac aaggaggagg aagcggaact ggtttgagcc aagtcgaagc tggagcagtt
     1561 actatcggaa actctgatgt atttgcagaa gaaagagacg gagttgatgc ttcaaaatta
     1621 gtggatcata aagtagcagt ggtcgggatg gcaccaatcg tcaataaaga tacagatgtc
     1681 aaagatatca ccaaacagga attgattgat atctttacag gaaaaatcac taactggaag
     1741 gaagttggcg ggaaagatca aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc
     1801 cgcgcgacat tcgaaaaatg gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa
     1861 gattcatcag gaacagttcg gcagctggta agtcaaacac caggagcaat cagctattta
     1921 gctttctcat atctcgatga ttccacacaa gcactaagta tcgacggtgt agaaccaaaa
     1981 gaagagaatg tagcagataa cagctgggaa atctggtcat atgaacatat gtacacgaac
     2041 ggaaaacctt cccctgaagt ccaaaaattc ttagactaca tgatgacaga agaaatccaa
     2101 gaaggacctg tcaaagaatt gggctattta ccaatcacaa tgatggaagt agaacgtgat
     2161 cacgaaggaa atatcaaata actagcagat aaacatgcca cggtagtcgt tactattaag
     2221 gaaaaatcaa ctatgtttcc ccacatatac atttttcctt taattgtgaa aaatagttcc
     2281 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttattc
     2341 gaaaaaaaga gctacgacat agaacttaag tagttcagtt ttggagtaca aaaagtgaaa
     2401 atgtaaacct aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa
     2461 atcaacgttt gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg
     2521 aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta
     2581 agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt
     2641 ctgtggattg ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg
     2701 cagagcgtac tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga
     2761 agtttattga atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta
     2821 tcaaagatag agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag
     2881 atgaaattgt agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac
     2941 aatatgattt attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc
     3001 caacgtcttt taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc
     3061 cgttttgttg taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa
     3121 gtcgatataa aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg
     3181 acaatcctat tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt
     3241 ctgttgttga agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag
     3301 attatcaatt tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga
     3361 agcaatttta caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt
     3421 gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa
     3481 agaacaccat tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta
     3541 cttgtgatat tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata
     3601 ggcgttgttt ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta
     3661 acgagatgtg gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag
     3721 gacaaggttc aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg
     3781 tatctgctga ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata
     3841 tttcaatgga tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt
     3901 ttagtcgtaa aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg
     3961 atagcgaaaa taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat
     4021 ttgtcgatgg tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta
     4081 aacaaatggt taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa
     4141 acgcctttta tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt
     4201 gccaagttaa caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag
     4261 cagaactgct cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg
     4321 catctcatta tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta
     4381 aagatggaga aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta
     4441 aggataaagg attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg
     4501 aaatcgcaaa gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga
     4561 taagacgtta tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga
     4621 gctatattta cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa
     4681 cttactacaa cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc
     4741 aaagattagg gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac
     4801 aaaaagagct tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt
     4861 aacgcacccg ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg
     4921 gatttttatc attctttatc cctttttggg caaatacctc attccattcc tcataattca
     4981 cttctggaaa aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct
     5041 caacataagg gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt
     5101 cgtttttaca taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg
     5161 taaacccctt atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg
     5221 attcaaggtt cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt
     5281 cccagtcctt gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat
     5341 ccatcgcagc aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat
     5401 ctaatttctt atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag
     5461 caattttttc tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt
     5521 atcattttca attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat
     5581 gaaaaaagta gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact
     5641 tacatagaac tgtcgtagct tttttttgct atatgacagg aatgaaaggt gtttgcaagc
     5701 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
     5761 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
     5821 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
     5881 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
     5941 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
     6001 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
     6061 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
     6121 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac
     6181 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
     6241 actggacatc aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag
     6301 gctttattga caggcgaagc agatgaaata accaaagaaa taggcgatcc attaatgtcc
     6361 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
     6421 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
     6481 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
     6541 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     6601 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     6661 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     6721 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     6781 aataaaatgt ctgttcaaaa agtgatcgtc tctaaaaaac aagaaataat cagtcagtca
     6841 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     6901 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     6961 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     7021 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     7081 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     7141 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     7201 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     7261 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     7321 gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     7381 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgctaaag
     7441 cgtcgtaaca atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa
     7501 gaagcagact gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa
     7561 gtggtcttac ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc
     7621 gttgtgaaca atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt
     7681 ttgctctcac tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc
     7741 acattgatta gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac
     7801 aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga
     7861 ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat
     7921 aaaacagatg tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc
     7981 tataaaatct gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc
     8041 ggtaactttt tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct
     8101 gtatcacttt tgctgttgag cattctattt ttctcggcag attcgatttt taggcatttg
     8161 accacattgg tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt
     8221 cgcgtagtta aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa
     8281 aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact
     8341 ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga
     8401 acctgttcca agatgactgt ctacagtaat cgtaccatga aggagttgca cgtagtcttt
     8461 tacaatcgct aaccctaatc cggtcccgcc tgaatgccgg cttcgcgcct tgtctacgcg
     8521 gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg
     8581 gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt
     8641 tgaatattgg acagcatttt caatcagatt tttgataatt ggataaagga tatctgtttt
     8701 agtagtaaat ggttcgttct ttccaatcaa tcgaattgtc aaccgtttct tttcgatgat
     8761 tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc
     8821 gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg
     8881 gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc
     8941 accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg
     9001 agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc
     9061 atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa
     9121 gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg
     9181 agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg
     9241 ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa
     9301 acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata
     9361 taattgctct tctgtggtag tatacgcccg atacatctta ctcatttgtt cgctcaacgc
     9421 attgatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg
     9481 attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt
     9541 ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa
     9601 aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat
     9661 acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc
     9721 cccattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc
     9781 tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa
     9841 gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc
     9901 caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag
     9961 ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg
    10021 ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg
    10081 ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta
    10141 atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta
    10201 acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc
    10261 aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat
    10321 tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca
    10381 gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga
    10441 ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt
    10501 ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct
    10561 ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc
    10621 tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct
    10681 aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt
    10741 tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt
    10801 ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta
    10861 caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga
    10921 aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat
    10981 gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc
    11041 atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt
    11101 ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa
    11161 gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa
    11221 cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat
    11281 gacgggagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt
    11341 cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat
    11401 ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac
    11461 agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat
    11521 cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa
    11581 gatcttggag gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca
    11641 ggtattggaa aatatgactg catatattac agataagtac ccggaagata aaaaactact
    11701 tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg
    11761 gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga
    11821 aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa
    11881 gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta
    11941 ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa
    12001 gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca
    12061 actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta
    12121 gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat
    12181 cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt
    12241 gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa
    12301 gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca
    12361 ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt
    12421 gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta
    12481 ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta
    12541 tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact
    12601 gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg
    12661 ctgacattga ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca
    12721 cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg
    12781 atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct
    12841 ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat
    12901 tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga
    12961 attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag
    13021 gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac
    13081 caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc
    13141 aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca
    13201 gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca
    13261 attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt
    13321 atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc
    13381 cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa
    13441 agtgaagcat tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga
    13501 gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc
    13561 gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt
    13621 tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa
    13681 gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga
    13741 ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc
    13801 aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc
    13861 gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca
    13921 acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca aaccacttat
    13981 gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca
    14041 aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc
    14101 cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta
    14161 aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc
    14221 aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta
    14281 ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta
    14341 gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata
    14401 aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg
    14461 ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg
    14521 gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt
    14581 tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg
    14641 tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc
    14701 tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt
    14761 caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc
    14821 cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc
    14881 ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag
    14941 caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat
    15001 aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct ataataatct
    15061 tacaaaagat aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga
    15121 tagattactt atttttaaca aaaaagaagt ctaaaatctt ctatttccta aaaaaaatcg
    15181 aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac
    15241 atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa
    15301 cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct
    15361 cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa
    15421 gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac
    15481 actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc
    15541 tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcattc
    15601 gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt
    15661 acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc
    15721 gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag
    15781 tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc
    15841 gagtgcagct tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca
    15901 agaaagcgta tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt
    15961 attccatttg ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca
    16021 catctatcaa gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg
    16081 gcttccacca ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac
    16141 cagttgaaag aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc
    16201 tatctcaata tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca
    16261 gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat
    16321 ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag
    16381 atcgacacaa atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt
    16441 gcagcaggtt caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg
    16501 aataatatag aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa
    16561 gaaaacagtc taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg
    16621 acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat
    16681 caagatccta tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc
    16741 gctgatctga gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg
    16801 catttttgca taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca
    16861 tgctaaaata aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt
    16921 aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga
    16981 tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc
    17041 acctgtggga ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt
    17101 tgaaggacat ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc
    17161 aaaagacaac tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct
    17221 gcatgtatta gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc
    17281 caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc
    17341 ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg
    17401 actgattgac gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta
    17461 tgggggaatc catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta
    17521 acttccgcga ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg
    17581 gagcaggtat gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag
    17641 ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc
    17701 agaaaatggc agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg
    17761 agggatatga tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg
    17821 tccttactgg gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc
    17881 cagatgtgtt agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg
    17941 aacatcccca atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga
    18001 caaatggaaa tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct
    18061 atcaacgtcg gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt
    18121 tagaagaaat caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc
    18181 taaaatcttc tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca
    18241 cttaactatt acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga
    18301 agaaaaccat gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc
    18361 cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg
    18421 aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa
    18481 cttatacagt acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata
    18541 caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt
    18601 actacctacg tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat
    18661 tgttatcaga ctgagaaaga acccttggta ttcaagggtt cttttttctt agcatgtaat
    18721 ttgaacaccc tgatccagtt ttttctttaa ttattggtag gggagaataa tggattagaa
    18781 taaatctacc tataagtagt agtaaaaaaa agagcactat gataatatct ttcttaagag
    18841 taaccgcttt aataaaaaat ccagaatatt ctaacataaa tggagtattc ttagaatcaa
    18901 aaggagaaag aagatggttt atacttatac ggagcaagaa aagatgaaag ttttagacac
    18961 attcatgaat gatggaaaag taaaacaagt accaagttca acaaaaaaga aatacatcct
    19021 attgaaagaa gtcttgaaac gctttgacca tggagtgact tatacagaaa cagaggttaa
    19081 ttcgatactg ctgaacgtat tttctagtgg cgattatgtc gaacaaagac gttatctaat
    19141 cacatttggc tttttcaaaa gaagttcaga cggtcgggca tatcagatga tgggaattga
    19201 aaattagaag ttttaattta tactcattga aaaaagttca agtatgcgat attggttcta
    19261 caccgcatac ttgaactttt tttgtcatct gtatctataa tacaaagcca agaacgttat
    19321 tcaaaatatg gatagctatg ataaaagcta atgagcgatt cgcagttaaa taagcaatcg
    19381 tcagtaaaat cgttccagga gcgtacatga aaaaatcaat tggatgcgtt acatgaataa
    19441 agataattct cttttatttg tacgaataaa taccaaaacg tacctcctaa aattagattt
    19501 tggacagatg ccctttaaaa agataacaag atttcgttct tgaaacaata tatatgcttt
    19561 tattccaatg aggttgtgaa aacagtattt tgtcctgagc gttagaaaat tgaaaatggg
    19621 gaatagcaac tcgtacttta ccatttttta agtgaataca agatcagcta attgaacacc
    19681 gtttattcgt gatgagggac acaacactgc agtcgacaca tttctct
//
LOCUS       DANKHK010000045        19544 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000045 DANKHK010000000
VERSION     DANKHK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            141..800
                     /locus_tag="PW109_001502"
     CDS             141..800
                     /locus_tag="PW109_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2511335.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            885..1790
                     /locus_tag="PW109_001503"
     CDS             885..1790
                     /locus_tag="PW109_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2511336.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1792..2424)
                     /locus_tag="PW109_001504"
     CDS             complement(1792..2424)
                     /locus_tag="PW109_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2511337.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2575..2700
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2744..3268
                     /gene="infC"
                     /locus_tag="PW109_001505"
     CDS             2744..3268
                     /gene="infC"
                     /locus_tag="PW109_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2511338.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3340..3540
                     /gene="rpmI"
                     /locus_tag="PW109_001506"
     CDS             3340..3540
                     /gene="rpmI"
                     /locus_tag="PW109_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2511339.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3593..3952
                     /gene="rplT"
                     /locus_tag="PW109_001507"
     CDS             3593..3952
                     /gene="rplT"
                     /locus_tag="PW109_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2511340.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4204..5541
                     /locus_tag="PW109_001508"
     CDS             4204..5541
                     /locus_tag="PW109_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2511341.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5589..7718
                     /locus_tag="PW109_001509"
     CDS             5589..7718
                     /locus_tag="PW109_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2511342.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7693..8382
                     /locus_tag="PW109_001510"
     CDS             7693..8382
                     /locus_tag="PW109_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511343.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8790..9476)
                     /locus_tag="PW109_001511"
     CDS             complement(8790..9476)
                     /locus_tag="PW109_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2511344.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(9612..9806)
                     /locus_tag="PW109_001512"
     CDS             complement(9612..9806)
                     /locus_tag="PW109_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511345.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9856..10296)
                     /locus_tag="PW109_001513"
     CDS             complement(9856..10296)
                     /locus_tag="PW109_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2511346.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10300..11145)
                     /locus_tag="PW109_001514"
     CDS             complement(10300..11145)
                     /locus_tag="PW109_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2511347.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11294..12712
                     /gene="pepV"
                     /locus_tag="PW109_001515"
     CDS             11294..12712
                     /gene="pepV"
                     /locus_tag="PW109_001515"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2511348.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12769..13716)
                     /locus_tag="PW109_001516"
     CDS             complement(12769..13716)
                     /locus_tag="PW109_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2511349.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13939..14289)
                     /locus_tag="PW109_001517"
     CDS             complement(13939..14289)
                     /locus_tag="PW109_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2511350.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14489..15568
                     /gene="pepA"
                     /locus_tag="PW109_001518"
     CDS             14489..15568
                     /gene="pepA"
                     /locus_tag="PW109_001518"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2511351.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15712..16032
                     /locus_tag="PW109_001519"
     CDS             15712..16032
                     /locus_tag="PW109_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2511352.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            16054..16518
                     /locus_tag="PW109_001520"
     CDS             16054..16518
                     /locus_tag="PW109_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2511353.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16723..17328
                     /locus_tag="PW109_001521"
     CDS             16723..17328
                     /locus_tag="PW109_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2511354.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17367..18656)
                     /locus_tag="PW109_001522"
     CDS             complement(17367..18656)
                     /locus_tag="PW109_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2511355.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            19084..>19544
                     /gene="rlmH"
                     /locus_tag="PW109_001523"
     CDS             19084..>19544
                     /gene="rlmH"
                     /locus_tag="PW109_001523"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2511356.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAG"
BASE COUNT         6191 a         3604 c         4017 g         5732 t
ORIGIN      
        1 cacttttttc ttaagaattt tttgactttt tttcttcttt gactagaaaa aaatacaatt
       61 gacactacta gatattgggg gtatatttaa aaaggttgga gttaaacaca atatttagtg
      121 atgaagtgag ctggaggtaa atgagcgtga tcgttttagc aggtacaatt ggtgccggaa
      181 aatcaagttt aacagaaatg atggctgaac actttgatag tcaggcattt tatgaatcga
      241 ttgatgataa tgaagtcttg cccttatttt atgcaaaccc tgaacagtat gcttttttgc
      301 tgcaaattta ttttttgaat aaacgttttg caagcatcaa acaagcaatg aaagatgaca
      361 ataatgtatt ggaccgttcg atttatgaag attcactgct tttccatcta aatgcagatc
      421 taggaagagc aacagaaaca gaagttcgtg tgtatgatga actgctggaa aacatgatgg
      481 aagaacttcc ctatgctgcc cataaaaaac atccggattt attagttcat attcgtgtat
      541 cttttgatac gatgttggag cgtatcgaaa aacgtggtcg ttcgtatgaa caattgtcct
      601 ttgatccaag tctatacgat tattacaaag aattgaacag acgttacgat caatggtatg
      661 aagaatacaa agaaagtcca aaaatccaaa tcgatggaga ccgctataat tttgtggaag
      721 atcctcaagc aaaagaagaa gtcttgaaaa tgatcgaaga aaaattagca gagattcgcc
      781 aaaccaaagt agccatttga tttacagttg ctcaaataaa gggtaaacct ttatttgagc
      841 gacttttttc tttatactgg aaacaaatgt caggaggata gagtatgcgg gaaaaacact
      901 attttaatca agaccgtaat ttttggatca atgtacagca aaacgatgtg ctagctgtta
      961 aaaacttaaa agaagaatat ggcattagtg acgagatgct gacttattct ctagataaaa
     1021 atgaacgggc gcgcgtagag tacgattctt ttgatgaagc gttactgctt gtatcaaatg
     1081 ttccgcacca gcaaaaagtc gataatcatt atgaaactag tcctatcgct tttattttaa
     1141 aagaagacgg tttgtttaca tttaccacgc cgaataccga gtacgtgatt cgactaattc
     1201 gatcgctttt ggaacgaatg ccagacatga gtgtatacag tttattgttt cgtacattgt
     1261 ttttgatttc tgattccttt tttcctttaa tcgaggaagt gaatagtgaa cgtcagcggc
     1321 ttaatctgaa attaagagaa aaaacaacga acaagaatct gctgcaactt tcagatttag
     1381 agatcggttt ggtttactta gttacaggaa caaaacaaaa tgtagtactt ttagaacaaa
     1441 tcaaggcatt ggctatttat cgcaagttgt cagaaaagga aaaagaagag ttggacgatg
     1501 cgctgatcga agcacgacaa gcagttgaaa tgacgaactt agcttcgcag attttagacc
     1561 agctctctgg cacttataat aatttgctga ataacaactt gaatgacacg atgaaatttt
     1621 tgacggtctg gtcgctgatc ttgaccgttc caactattgt gacaggattc ttcggaatga
     1681 atctccagtt accctttacc cattccgttt tcggatgggg aatcgccttg atcatcagtt
     1741 tggtcttatc tatttggatg cttattgcac tgtggcggcg gattcgttga tctagtcttt
     1801 gatctgcaaa ccaagggctt gggcgatcac aagtgcttgc tcttggttga cgattagtcc
     1861 cgccaaagaa tcttgagata aagcgattgc tgtgaagaaa ttagtcgtaa agtctaatcc
     1921 cttcagagaa gttcgaaacc agttcgaacc ggtcaactga ttgtcagaca aaaaaaggtt
     1981 tttccaagtg acctcataaa attcgctgtc ttctaattga caatgatcga aggagacagc
     2041 tttcaagttt gtactactaa aggaagccat cgaagcaata caatcagtga aagtacagtc
     2101 tcgaagataa ctttcggcaa aattcgtacc tgttaattta cattgatgaa acacgacttg
     2161 gtggaaactg ccaccaatcc actccagatt ggaaaaatca catttttcaa aaatgacatt
     2221 actgcattca aaatgacgta aaactgtttt ttgcatcgta agtttttgga tatgactctc
     2281 tcttaatacc atatttttgg catcgagata gctttggtca cccccgttga tcagaagacc
     2341 agagaagatc tcctcatctt ctaaataaaa ctctcccttt gtaagaagag gaagagaagg
     2401 tggaataggt tcgatttttt tcatgtgggt tcacctcgtt tttctttagt atagcaaaga
     2461 agcgggaatc actgctagaa agtctgctaa tctaatcatt ttcagaaaag gaagacaaac
     2521 tgtatgaaaa acattgacaa aaagattagt ttttgctata ctatgatggt tgagaaataa
     2581 aagcagaagc acccgcttct cgccttagtt gataatgtca ctgggctaga tagttttcac
     2641 tggttctatt aggtgaagta cgcgcgggtt ctgtaagaga actcgttttt ttattgccgt
     2701 ttattcggta agcttttctt tctctcataa tatttggagg tgaatgacca tagcaaagga
     2761 tatgatggtt aacgacggca ttcgtgcacg agagttacga ttgatcggtt cagatggtga
     2821 gcaattagga gttaagacaa aagcagaagc attgaatatt gcagaacaag caaacttgga
     2881 tcttgtgtta gttgcccctg gtgcgaaacc accagttgcg cgaatcatgg actacggtaa
     2941 ataccgtttc gaacaacaaa agaaagaccg cgaagcgcgt aaaaaacaaa aagtgatcaa
     3001 tgtaaaagaa gttcgcttaa gtcctacgat cgacttgaat gacttcaata caaaacttcg
     3061 taatgcacgt aagttcttgg aaaagggcga taaagtgaaa gcttctatcc gtttcaaagg
     3121 ccgtgccatt acccataaag aaattggtca aaaagtcttg aatcgcttag ctgaagaaac
     3181 tgcggatatt gcaacagtgg aacaaaaagc gaaaatggac ggacgcagca tgttcttgac
     3241 gctggcaccg aaaaacgaca gtaagtaatt tgaatgacta actggctgtt ggacagccga
     3301 tagtcggata acgaaagatt ttgaggagga aatatagtca tgccaaaaca aaaaacacac
     3361 cgcggatcag caaaacgttt caaacgtact ggtaaaggcg gattgaaacg cttccgcgcg
     3421 tttacaagtc accgtttcca cggcaaaaca aaaaaacaac gccgtcaatt gcgtaaagca
     3481 agcatggttt ctagtggcga cttcaaacgt attcgccaac aactagcaaa aatgaaataa
     3541 gaagtacaaa taacagaatc tgaagattta tttttattag gaggaattat acatggcacg
     3601 tgttaaaggt ggaacagtaa ctcgtaaacg tcgtaaaaga gtgctaaaat tagcaaaagg
     3661 ttactacggt tcaaaacaca ctttatttaa aacagcaaaa gaacaagtaa tgaattcata
     3721 caactacgca tatcgcgatc gtcgtcaaaa gaaacgcgac ttccgtaaat tgtggattgc
     3781 tcgtatcaac gcagcagctc gtatgaatgg cttgagctac tcaaaattaa tgcacggttt
     3841 gaaagttgct gaaatcgaca tcaaccgtaa aatgttagct gacttggctg ttaacgatgc
     3901 agcagcattt actgcattgg ctgaccaagc aaaagaagct ttggctaaat aagctaaaca
     3961 attataaaga ggaaagaaga taacgaaaag ttatgttctt tcctcttttt ttatttacaa
     4021 tcctccatca aatgaaacag gacaataacg tataagtcta aaatagcata attaaggttt
     4081 tttaattgaa aactgaacat ttatggtcac cctttaatga aagcgctaac tacataagtt
     4141 agaatgagtt tgactgatat attagtttgc ttatcagaaa aacaaaatgg acggaggaat
     4201 aacatgagtg atattattat tggtattttg ttagttatct cattcttttt catggtctgg
     4261 tactgtgtaa aaggctacaa cttgatggtc ggctttgcca tcatggctac tgtatggatg
     4321 ggattagcgc tagtcggaaa cacgttttca ccaaacccgg cgatggaagg gcaaggagta
     4381 attgatgtgt tgacgcatat ctatacgacg gggccagctg agtacgccaa atcgatcttg
     4441 gtaaatgtct tctttggtgc ctttttcggg cgtgttctag tagatagcgg aatcgcagct
     4501 accctgattc gaaaggtagt tgaacttgga ggggacaagc caagaatcac gatgagctta
     4561 ctatgtattg taaccgctgt tatattcatg tcgatgacag gaatcggacc agtgatttct
     4621 attgcagtta ttgtgttgcc aatcttgatg tcattaggta tttcagtacc ggttgcactg
     4681 ttctcattta tgggctcgat tatggctgga atctttgcta atatcgtcaa tttcaaacaa
     4741 tatcaaacga tttatgctgg tttcaatcca gcggctgaaa gctatactta taatgactat
     4801 ttccaaattg gtatgattgg gatgattgta agcttagttg tggtactaac ggtcgctaat
     4861 atatcaatga acaaaaagaa acgctatgca atggcagcaa acgtaccagc tgaaggtggc
     4921 gacgcaccaa tgatctcatg gttagccgta ttattaccag ttttaggggt cgttctttta
     4981 gatcttccaa tcatcttagg ttttattcta gcaggtatct gggcattgct gtttactgga
     5041 aaattgcgcg gaggttataa agaaatctgt cgtcagtttg cgaaattatt tacagatggc
     5101 gcagtagatg ttgcgccaat ggttggtttc ttgatgacct tagcgatgtt caataattca
     5161 gctgcgtatg cttctcctta tttttcagct atttttggag attggatacc gcaatcacca
     5221 ttagtcttgg caatcgtatt tgctatattg acaccactag gatttttccg cggtccaatg
     5281 aatctagtcg gttcaggctc agctatttta gctgtcgtat tagctgttaa tccaacgatg
     5341 tcgccagcct ttttgttccc gttatttgca atcacaacga ttgctcctca gcatttagat
     5401 attacacaat cttgggttgc ttggggatta ggttatacaa aagtaacttc tcgagaatat
     5461 atgaagaaat ccatcccaac tggttggatc atcggagcga tcttatgcct gatcacgttc
     5521 ttattgtacg ggaacgccta gtaaacaaaa cgtcgataaa aacgattaga tgaatggagg
     5581 aaaagaaaat gaaacgtttt gtgcgaatgg gaattgatgt tggcggtaca catacgaaag
     5641 cagtcgcaat cgataatgcg acgcacgaaa tcatcggaaa atcttcagtg aaaacaaccc
     5701 acgatgatgt ccgtggagta gccgcaggtg tcgttcagtc ttttcaaaat tgtttgctgg
     5761 aaaataatat cagtccagaa gatgttgtgt ttgttgcaca ttctaccaca caagcgacta
     5821 atgcactgat tgaaggagac gtagcaaaag tcggcgtaat cgggatggca aaaggtggat
     5881 tagaaggttt tttagcaaaa cgacaaacta gattgaacga tatcgattta gggaataaga
     5941 aaaaaataga gattgtcaat gcattcttac ctgtcaaaca cttaaatgta gaccgtgttt
     6001 cagaaacgat ttcttcttta gaaagagaaa gagcggaggt cttggtttct tctatggcat
     6061 ttggagtaga taatggggaa ccagaacgag ttgtttatga agcagcatcg gtaaaatcga
     6121 tccctacaac gatggcttct gacatcacaa aattatatgg attgacaaga agaactcgga
     6181 cagcagcaat caatgcatct attttaccaa aaatgctaga tacggcaacc tctacagaag
     6241 attccgtacg agaagcaggg gtcaatgttt ctttgatgat tatgcgcgga gacggcggag
     6301 taatggaaat caatgaaatg aaaaaacgac ctgttttaac catgctttcc ggtccagcag
     6361 catcagtcat gggctctttg atgtacttga gagcgtctaa tggtgtttat tttgaagtag
     6421 gcggaacaac aaccaatatc ggggtcatta aaaatggacg tccagcaatc gattattcaa
     6481 ttgtaggtgg ccacccaaca tatatctctt cattagatgt tcgggttcta ggtgtggcag
     6541 gcggttcgat ggtacgggct aatcagtcag gaatcatcga tgtcggtcca cgttctgcac
     6601 atattgccgg tctagattat gcagtattta cggagacaga aaaaatcaaa ggtcctaagg
     6661 ttgaattttt ctctcctaaa gaaggagacc ctgctgatta cgtcaaggta gtgatggaag
     6721 acggagaaga agtgactatt acaaatacat gtgcagccaa tgtccttgga ttagtgcaag
     6781 aagaacattt ttcttatgga aatgtcccat ctgcacgaaa agccatccaa gcattagctg
     6841 attattgtca tacgacggtt gaagacatcg cagaacagat catggaaaaa tcctatgcta
     6901 agattgaacc ggtgatttta gaattggcag acaagtatca cttggagaaa gatcagattt
     6961 ctcttgtagg tgtagggggc ggcgctgcct cattgattac ttactttagt aataaaatgg
     7021 gtgtgaaata ttcgattcct gaaaacgccg aagtgatttc ttctattggt gtagcacttg
     7081 caatggtccg agatgttgta gaacgtatca tcccttcgcc aagtaaagaa gatatacgat
     7141 cgttgaaaaa tgaagcgatg aataaagcga ttgaatcagg cgctacacca gaatcgatcg
     7201 aagtccacgt agaaatcgat ccacagactt caaaagtaac ggctatcgct actggttcta
     7261 cggaagtgaa agcaaccgat ctaaccaagg agatcacaac agaagaagca ctagagcttg
     7321 ctgcagagga tatgcgatta aataaaaatg aagtttgctt attggaaaat acacctttct
     7381 tttatgtatg cggcgaacag aatcgttcaa aaaatgctgg aagtcttcgg atcatcgatc
     7441 aaaaaggatt tatcaaggtc caacgtggac atgcttcatg tatgaaaaca actgcagcca
     7501 attatatgac agctgtcgaa caactttggg aagatatggc agtttatcaa actgaattga
     7561 ttgcccgacc agaattttat ctctgcttag gagcaagagt ctctgatttt acagcgacgg
     7621 atctggaaca actacagctt ttgatggatc tggaagtatc tactatggaa ccggaggaag
     7681 aagtgatcgt cgttgccgga aatatcaaac aaacataagt tagaagagtt actaaacgaa
     7741 ttacaacaga tccccgaaga aatttgggga ttctaccagt ttcggcgaga tttattttgg
     7801 aaaaagattc ctttgtccaa gcagaaaatt cttatacaac aatcaatcga ttgtggtatc
     7861 gaaacggcat gtagcatcaa aaaaaagtac ccttttgccg atgtaggaga aatttgtgaa
     7921 caaatggctg ttccaatcgt ttcctgtgaa tcagagcaaa taaacgaacg aatcactttt
     7981 gcaacttatg cagaagatga agggattcga ttgatgacgg agccgttaga aaaattaaaa
     8041 tgttcaggac tagcttcgat ctctaaagaa acagcgcaag ctttgattat tggacatgaa
     8101 ctatttcacc acatcgaagc ttctgttaag ggaatttata cgcaaagcga aaaaattgtg
     8161 ctgtggcggt tgccttttta tacacatcaa tcaaacattc gtgcattgag cgagatagca
     8221 gcgatgagtt tttcaaaaga gatgaatcaa agcgattttt cgccttatgt attagaggca
     8281 gtcttacttt ggccatataa tgaaattcgt agccaaggga tcttagaaga aataaaaaaa
     8341 atagaaaaaa gatgtacgga gtatgattta gattacaaat aaagagactg tgacaaaagt
     8401 cgtgtcacag tccttttttt ccgaataaac ggtgttctcc tgtcaactcc tcggtcacaa
     8461 gtcaaaatat tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg tgactcggag
     8521 atgaacggca ggctcacgac ctcttggaaa acggtgttca aaagcttgca tctgcggtaa
     8581 taagtccagc caagttaaaa acataagaag ctatttttac ttggctgttc ttattacttg
     8641 atgcagggca gctttttcac aacctctcaa taaacggtgt gcaatcagct gaccctcggt
     8701 attagctcaa aaaaaacggc aaaatatgag gaactatttt gccgtttctt tctctaatac
     8761 tcaggtcaaa acgctgattc caaaacctct tatttgatgg gtctgtcctg aatatcttca
     8821 aaataatcgg ggtaattatg aatcacgtat tcttgctcct cgataatcat atgtaaatgc
     8881 atcgtgcaaa gaaatccgac ttcatcaaag ttatgatcaa tcattgattg aagcattgtc
     8941 tgatgttcat ctaatactcg accccagtct aattctgaaa tcgtcaaacg taaccatctg
     9001 aaacggttca agtgcgtact ataactttct agccaatccc aaatttctcg ttttccagca
     9061 atttcataac aagttcggtg aaaatcagta tccaaatcaa aaaaagcttt tttattttga
     9121 gcgattactg cctgatcggt ttgttctagt attttttcta gtttagcttg atcttgcttg
     9181 gtcattttcg cactgcattc ttgcaggatc ggtttttcca acttttcccg cgtataacgg
     9241 gctaatgaag cagatcggat atcgattttt gaaacataag tgccggactg tggaatggtt
     9301 tttacaagct cttgattgcg cagttggatc agtgcttctc gaatcggtgt acgcccgata
     9361 gctagttgtt cttctaacac tttatcggag attttacttc caggtgctaa ctcggaataa
     9421 atgatttgtt tacgtataga ttcataagct tgctgttgca aattattgat aaccatttaa
     9481 ccttcctttc tatgcagata taatctatta taacataaat cgcttacaaa tcgtgtgggc
     9541 ataaaaacac agtctgcaaa aaaagccctg ataggcatgc ctattagagc ctcgaaaaga
     9601 aaaatattga atcaatctgg tcgtacctct tggatcggtt tgacacgacc gccgattctt
     9661 ttagcaaaag cttcggattc ttttttgtct ttgaaaatga gcagattgtc taagtttttt
     9721 tcccgagtgc gacagccagt tttatcgatg taaccgtgca ttactttaac gacatacatg
     9781 gggatcacct gcttttttct tttcattata cgaactactc gacacaatac aagaaaaatg
     9841 cttagacggt ctttattaag aatttacatc atccaaagaa gcgtacgctt ggaataactg
     9901 atctaactcc ggattgactt tgctcagctt cactaatcgt tgcgaatcag aaagtaaaaa
     9961 acagtgttta ctactgccag tagtttttaa ttttttgtct tcagttccca caatttcgta
    10021 gctaaaatcc aaccgtgtcc ccgtatactt ttggatagtt ggatggatgc tcactttttc
    10081 tccgtaatgg atcatttctt tatagctgca atttacttcc agaacgggga tgatgatccc
    10141 agattcttcg atttttttat agggaaaccc tagatgtacc aataaggcta cccgagcctc
    10201 ttcaaaccag cggatataat tgctgtgatg aataatcccc atttgatctg tttcataata
    10261 aaaaggttct cgcacataac ctgtaaattt ctccatgact catgcctcgt tttccattga
    10321 ttttttacaa aaataattca tccatttcgg aagtgcttcg ctgatttgtg tcggcaaaac
    10381 aatatattgt tcttttgcca gctggtctcc aatgaaacta tgaagataaa tagctgctgc
    10441 aatcgtttgg tcattttgag gaaactgggc taaaaatcct gtgatcatcc cagctaaagt
    10501 gtcacccatg ccgcccgttg ccatagcagc agaacctaaa ggattggtgt agaagtgctg
    10561 gtgaccataa atttttgtct catgactttt caaaacgagc acagcaccga tttcctgttg
    10621 cttctttttg ttttctttat cccgttgttc ttgtatcggt agagaactca accgttgcca
    10681 ctccatttga tgaggtgtaa gtacagtttg ttcgggatag ggtagcaaaa gatgattagc
    10741 agcaagtaaa cttaaagcag agccatcaat caccagccat tgttcttttg attggttttc
    10801 tagtacttgt ttgaaacgtt ccaaactctt agaagaagta cccaatccgg gaccaatcaa
    10861 gagtacatct gcttgcttta ttacggaaag aaactggtca gtatccgtcc aaactacaat
    10921 cattgcttct ggcagatgac tgtgaagagc actccgattt ttttcatcag ttactacagt
    10981 cgtgagaccc gctccgctgt gtacggcagc taaagcactc ataatgattg ctccgccata
    11041 ttgttcattg ccgccaatca aaacgatacg tccaaatgtt cctttatgtg aaatttttgg
    11101 acgaggtgtg atgactcttt tcactagttg ttcgtttatc agcatctaaa aacctccttt
    11161 tactcattat agcgtatatt ttttttagag aaaaacagat aagaaaagga attgtcagat
    11221 tttttgaact atttttttct gtaatttgtg gtatcatgaa cacgaagact taaataagtg
    11281 gaggattaac tttatgacaa ttgattggca aaaagaagta gaagcacgta aagaagactt
    11341 gcttgaagac ttgaaaaatc ttttacgtgt caacagcgag cgcgatgact caaaagtaac
    11401 accagatgca ccatttggac ctggacctcg cgacgcattg aaacatatgt tagcttatgg
    11461 cgaacgtgat ggatttaaag taaaaaatgt cgataactat gcagggcata ttgatcttgg
    11521 cgaaggagac gaaacgctag ggatatttgg acacatggat gtcgttcctg caggagatgg
    11581 ctgggacaca gatccttatg aaccagtaat caaggatgga aagatctttg cacgcggatc
    11641 aagcgatgac aaaggtccaa gtatggcagc ttactatgcg atgaagatca ttaaagaact
    11701 tgacttgaaa ctatcaaaaa aagttcgctt cgtagtcggc agcgatgaag aaagcggctg
    11761 gggcgatatg gcttattatt tcgaacatga agaagaacca gattttggtt tctcaccaga
    11821 tgctgaattc ccaatcatca atggtgaaaa aggaaatgtt tctttagctt tacgcttcaa
    11881 aggcgacaat gcaggagatt atgtcttgaa atcttttgtt tccggacttc gcgaaaatat
    11941 ggtgccagga acagcaactg cagcacttca agtaccaagt gcagatgcag ctatcgcgat
    12001 ggaagaagca ttttaccaat tcatcgaagc aaatccagta agcggaacga ttgaagcgga
    12061 caatacgtat gtaaaaatcg aattagttgg taaaggcgca catggtgcaa gcccacaatc
    12121 tggtatcaat gcaggttctt tcttggcatt gttcttagat aactatgaat tcctaggatc
    12181 tgctaaacaa tttatccatg tagcagctgc ttatgtccat gaagattttt atggagaaaa
    12241 actaggagtc gcttatgaag atgaaaaaat gggcaaattg acaatgaatg ctggactatt
    12301 tgcttttgaa gaaaatggta cagaggaagc gaacttcatc aatatgaatt tccgttttcc
    12361 aaaaggtgta acagtagacg gattgcaaag tgacatcgaa caaacagtgg ggcaagaagg
    12421 cgcaacagtt actcgaggtg ctcgcgtaat ggaaccacat tacgtaccaa tggatgatcc
    12481 attggtggct acattattgc aagtctatga agaccacact ggagaaaaag gctacgaaca
    12541 aatcatcggt ggcggaactt acggacgttt gttgaaacgt ggcgttgctt atggagcgat
    12601 gttcccagga tatactgata caatgcatca agcaaacgaa tttatgagtc ttgatgactt
    12661 gttcagagca acagcaatct atgcagatgc catctatcgt ttggcgaaat aattaattaa
    12721 aaagaggatc aagaattgca aattattttt gcagtcttga tccttttttt actttctact
    12781 gtggtattca tgataaacat gatatagatt caattcatat tctttcttag cgttcgtgac
    12841 gactgtatcc aaaatcaacc ctgttactaa tgaaagaagt gctaaagtca tcaatccagt
    12901 acttaaaatc gcagaaggga tgcgtgtaat aaagcttgtc agcatatatt cgcgaatcac
    12961 aggaatcccc gtgaccaatc caaataaaaa gaagaagatg gtccaaatcc caaagaaaag
    13021 caaaggctta taatctttaa acattttaat aatcatcatg atcactttga atccgtcaga
    13081 gaatgtattc aattttgatt cgctgccttc aggacgatca cgataatcga ttgggatttc
    13141 aacgaacttg aattttttat ctaaagcatg gatcgtcaat tccgtttcga tttgaaaacc
    13201 agaactcata ataggaaaac ttttgacaaa gatacgattg aatcctcgat agccggtcat
    13261 gacatcaaga atttttgttt tatacattct agtaattgta tttctaacaa ggttatttcc
    13321 aaaatcgtgg aaaggtctct tattctcatc aaagtaggtt ccatttgata ggcgatcacc
    13381 aatgaccata tctgcttcac cagaacgtaa tttctcgagc aaaccatgta cagcttctgc
    13441 aggataagta tcatcgccat ctaccattaa ataatagtcg gcatcgatat caaagaacat
    13501 ttggcggatc acatttcctt ttccttgacg aggttctttt tttacgattg ctccaccttg
    13561 aacggccaat tcgtatgttc gatcggtcga attattatca tagacataaa tatctgcttc
    13621 tggtaattct cgtttaaagt ctgcaatgac tgttgaaatt gttgcttcct cattgtaaca
    13681 aggaatcaga acagcaattt tttcttgttg ttccattttt cactctcctt aactatagaa
    13741 aacaaaagct aaattaaaaa tacctcataa tcaaaggaaa tgctagtttt tcccgctaaa
    13801 tatcatttat ttaaaaagaa aatactggaa tatatgtttg gaaagctcta tctaaaaaac
    13861 cacccactac aaaagaagga catgatacct ctcttttgaa tagtgggtgc gtattcgttc
    13921 tctcactttt ttgacgtatt aatctgttgc accttttttt cttgaaattt ctaatacggt
    13981 tcctgtatga gcatctgcta aaaattcata ataaacagtt tcgccatctt ctattcgaga
    14041 aagacctccc cgataagcac tcgaccgaac ggcgaatttc ctaataggtt ccttttcaaa
    14101 agagatccaa gaaccttcga ttgcaccttc ttttagaaaa gatgacttga tattttcaag
    14161 tacttggtct gctgacaata cttttttgcg atgataccaa atcgttgaag ctaccccgct
    14221 caataatcct agaccgattc caactgctaa cccgccttta aaatagctta gttcgctttc
    14281 ttcatacatg tggacatacc tccttcttct cacttgttac tccttgtgct tattttacca
    14341 cactctcata ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat
    14401 acgatcggtc tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag
    14461 tttagtctga aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga
    14521 gttaacagaa ttacaaggaa caagcggttt tgaacaagat gttcgcgctt atatggaaag
    14581 tcatatggaa ccgctggtag atgagttaca gttagatggt ttgggcggca tttttggtct
    14641 gcgccatcat gaagaagcgg atgcaccccg tgtaatggtc gcagcacata tggatgaagt
    14701 cggattcatg ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg
    14761 ctggaatcct tacgttgttt ctgctcagcg ttttacactt aaaacctcta ctggtaagaa
    14821 ttatccatgt atttcttctt ccgttccccc acatttattg cgaggaacaa gcggacaaaa
    14881 atcagtagaa gtaaccgata ttttgttcga tgctgggttt gaatcaagag aagaggcaat
    14941 gagttacggt gtccttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc
    15001 caacggaaag aatattatca gtaaatcatg ggacaaccgc tatggctgta caatggtatt
    15061 agaagcttta gaagcgttgc aaaatgaaac attaggtcat acattgattg caggcgcgaa
    15121 tgtccaggaa gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga
    15181 tctgttcttt gcagtagact gctctgcagc agatgataca gtgacgaaaa atggcacatt
    15241 cggacattta ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc
    15301 tcgtttgcgc gagtacttgc tggatatcgc tgaaacaaac aatatccctt atcaatactt
    15361 tgtttcaaaa ggcggtacag atgcaggtgc tgctcataca caaaacgaag gaatcccaag
    15421 tacagtgatt ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg
    15481 cgattttgaa gctgcacgag aaatgttgat tcaaacactg aaaggtttgg ataaatcaac
    15541 agtaaatacg atcgtatacg gaaaataatg cgatagtgaa actataaaat ctggcaaaag
    15601 attgcttttt gccagatttt ttttataaaa agaggatcaa tgtttgttag ttcgaaagaa
    15661 aggtgtataa tttaattgaa aaatacataa acagaaagta gaggacaaag aatgattatc
    15721 ccaaatagtt tagaagaatt agcaggctac gtagaaactg gaaagagtgt atttttcttt
    15781 actgccggct ggtgcggaga ctgccgtttt atcaaaccgc aaatgcctga aattgaaaat
    15841 gacttttctg attggcaatt tattgaagta gaccgtgaca agtacataga tgtagccgct
    15901 gaatggaaca tttttggtat cccaagtttt gtcgtgatcc aagacggaaa agaattaggt
    15961 cgtctagtga ataaagatcg taaaacaaaa caggaaatcg aatcgttcct agaaagtgta
    16021 acaaaaggct gacatctgtt aggaggggtc aagatgaatc aagaatataa gaatatttta
    16081 gtaggtatcg acggtagtga acaagcactt gctgctttca aaaaagcggt agaagtggct
    16141 cgccgtaata atgggaaagt atatgtagca aacgtaatcg atcagcaatt ttataacttt
    16201 atgggctatg cacctattga tcaaaatcta gtcgatcagc aaactgaaac agccaaggaa
    16261 atgatcgaag aatgtaaaga atatgggaaa tctgttgatt acacaaatat agaaggaatt
    16321 attgcatatg gttctgttcg tgaatccatg gcagtcaaat tgccaaaaaa atatgagatc
    16381 gatctaatca tggttgggca gtcaggtcta aatgcagtcg aacgctttat gacaggaagt
    16441 gtagccagct acgtgatacg tcattcacct tgtgatgtct tgatcgtttc tgacgaagaa
    16501 gaacagccaa gtaattaagc aagtggaact tgtacgacct ttttaatcaa ttaactaaaa
    16561 cagtaaaggg actgtgacaa aagtcttgtc acagtccctt gttacgaata aatggtgttc
    16621 cagaagaggc ttcttcggaa ataaagcatg aaaaaaattt catgcagtga atgcaatttt
    16681 ttgttaagat aaatctcagc aaaggataat ggaggaaaga acatgatttt tgcttataac
    16741 aaagcacacg tcggagacac cttgatggtt attatagccg acgataaagg aacagaaaac
    16801 actgttgaac gcaaatggaa tgttgctcgg atcaaagatg aacaaggtca agttgttgct
    16861 tggaactttt tccatatctc cgatcatcta acaatcgaag ggaacggcca agtaagctta
    16921 acagatgagg aattagcgaa attgaatcga ttgatcaaag aagcaggatt cgaagaacag
    16981 cttgaagctg atcatgaacc gaagatcgtt gtcgggtacg taaaaacatg cgtcccacat
    17041 cctgattctg accatttatc gattactgag acagaagtag acaatggtac agtattgcaa
    17101 atcgtttgcg gtgcacccaa tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga
    17161 gctatgatgc cagatggaat gatgatctgg ccaggaactt tgcgaggggt agatagctat
    17221 ggaatgatct gttccgctcg tgaattgcat ttgccaaatg ctccagcaaa aaaaggaatc
    17281 ttagtattgc ctgaggatgc cgtagtcgga gaagcatttc caaaataaag aggttgtgac
    17341 aagtctttgt cacaacctct tttttattaa ttttcactat tttgattttg ttttaatgcc
    17401 gattgatcga tcgtcaattc aacagtagtt gttttttctt tcgagccttc atagtaagtg
    17461 attttgacag aatctcccac ttttttctta tacaaagcag attgaagttc tacaccagaa
    17521 gaaacttctt tgtcatcgat cttagtaatg acgtcgtatt gtttcaatcc agctttttca
    17581 gccggtgtag ctgtttggac atttgtcacg atgacaccgt tagttacaga tgaaggaatc
    17641 ttcaagattt gttcttgttg ctgagtagaa acggcagata agtcgaccat tgtgatacct
    17701 agagctggac gtgtcacttt tccgtctttt tccagttgat tgatgatatt cactacatcg
    17761 ttacttggaa tagcaaatcc cattccttct acgcttacat tagaagtaga ttcagaagta
    17821 cttgcgatct tacttgagtt gataccgata acttgtcctt cgatattaac taaaggacca
    17881 ccagaatttc cagggttgat tgctgcatct gtttggatcg cattgatatt gactgtttcg
    17941 ttcgattcat tcgtacttgt cacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca
    18001 gaatttgcat attcagatcc tagtggggaa ccaatcgcaa tcgcaggctc accaaccttc
    18061 agagcacttg agtcaccaaa agaggcaact gtttcgactt tgtctgaatt gatcttcaac
    18121 accgctaaat ctgaataagc atctgttcca actaattcag cttttacctt cgtaccgtct
    18181 ttcatcaaga cttcaagacc ttgctggcca tctactacgt gattattcgt gactacataa
    18241 gcagtattcc cgtctttttt gtagatcacg ccgcttcctt cactataagc ttccaaatta
    18301 ctatcatctg agctgctact ttcttcttgc tgcccgaata gtccgccaaa accgcttgat
    18361 tggttttgac tttgtaagtt gataacagaa acgacagcgt cttgaacttt atctaccgct
    18421 ttggtgatat cagaatctac attgactttg acattttcca caactgtttc accggcagaa
    18481 ttctgattgc cgcttgtagc agtagaacta ttgttgccag tgcccatcgc tgcataaaaa
    18541 atgccagctg tcagtagtcc tccaacgatc cctccaacaa gccccagacc taacttacgc
    18601 cagaggctat tatttttact ttttttcatc tttggtgtca cattttttct atccatgtgt
    18661 tcctcctcca attacattca tttatctact gttagtatag tcttatttgt tgactttagt
    18721 ctactttgag actgtgaact ttttatgaaa aactttagga aaaatcattg ttttcagatg
    18781 aaaatatttg attttctttt tccacagata ctgtgtaaaa ctacgcggat aggattggaa
    18841 aaataaggga taaaacatat ccacagcctg tgtataaagt ggataaattc ttataaaacg
    18901 tatgtttgcc taatgaatgt gatgaaataa ggtaaaataa cactgttttc tgtggataaa
    18961 cctgtggatg ttgtgcataa ctgttgggga aatcttttta acaaaaatag ccaaaataaa
    19021 catatagacg aaaaaattga ttataataaa caaagtgagc atgaagacca aaggagagaa
    19081 aacatgaata tcaaaattat ctcagtagga aaattaaaag aaaaatacct gatccaaggt
    19141 atcaatgagt atgtcaaacg gctgaatgcc tatgcaaaaa tcgaattgat cgaagttcct
    19201 gacgaaaaag ccccggagaa tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa
    19261 ggggaacgga tcttagcgaa aatcaaagaa caagaatacg tctatgcctt agcaattgaa
    19321 gggaaaaatc ctacaagtga atcttttgct aaacaaatcg accaattagg tatccaagga
    19381 aaaagccacc ttgtttttgt tatcggcggc tctttaggac tcagcgaagc cgtcatgaaa
    19441 agaagcaatg ctcagatctc ttttggaaaa atgacgtatc cacatcaatt gatgcgtctg
    19501 atccttgtcg agcaaatcta ccgtgccttc cggatcaatg cggg
//
LOCUS       DANKHK010000046        19321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000046 DANKHK010000000
VERSION     DANKHK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..460)
                     /locus_tag="PW109_001524"
     CDS             complement(<1..460)
                     /locus_tag="PW109_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2511357.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKR"
     gene            complement(565..1428)
                     /locus_tag="PW109_001525"
     CDS             complement(565..1428)
                     /locus_tag="PW109_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2511358.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1624..3723)
                     /locus_tag="PW109_001526"
     CDS             complement(1624..3723)
                     /locus_tag="PW109_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2511359.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3943..5052)
                     /gene="rpoD"
                     /locus_tag="PW109_001527"
     CDS             complement(3943..5052)
                     /gene="rpoD"
                     /locus_tag="PW109_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2511360.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5076..6917)
                     /locus_tag="PW109_001528"
     CDS             complement(5076..6917)
                     /locus_tag="PW109_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2511361.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7099..9414)
                     /locus_tag="PW109_001529"
     CDS             complement(7099..9414)
                     /locus_tag="PW109_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2511362.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9599..10459)
                     /locus_tag="PW109_001530"
     CDS             complement(9599..10459)
                     /locus_tag="PW109_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2511363.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10518..11150)
                     /locus_tag="PW109_001531"
     CDS             complement(10518..11150)
                     /locus_tag="PW109_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2511364.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            11317..12078
                     /locus_tag="PW109_001532"
     CDS             11317..12078
                     /locus_tag="PW109_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2511365.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12104..14695
                     /gene="mprF"
                     /locus_tag="PW109_001533"
     CDS             12104..14695
                     /gene="mprF"
                     /locus_tag="PW109_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2511366.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14883..16070)
                     /gene="tuf"
                     /locus_tag="PW109_001534"
     CDS             complement(14883..16070)
                     /gene="tuf"
                     /locus_tag="PW109_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2511367.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16438..16794
                     /locus_tag="PW109_001535"
     CDS             16438..16794
                     /locus_tag="PW109_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2511368.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17035..18039)
                     /locus_tag="PW109_001536"
     CDS             complement(17035..18039)
                     /locus_tag="PW109_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2511369.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18205..19056
                     /locus_tag="PW109_001537"
     CDS             18205..19056
                     /locus_tag="PW109_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2511370.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19073..19318
                     /locus_tag="PW109_001538"
     CDS             19073..19318
                     /locus_tag="PW109_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511371.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEKLKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5387 a         3993 c         3375 g         6566 t
ORIGIN      
        1 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
       61 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
      121 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
      181 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
      241 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
      301 tttttttcac taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa
      361 gcacaagcag agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct
      421 gttttgtttt tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt
      481 ataataatta caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag
      541 ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt
      601 ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt
      661 gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg
      721 ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc
      781 atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg
      841 accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc
      901 aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc
      961 tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt
     1021 ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa
     1081 gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac
     1141 aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt
     1201 aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt
     1261 ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc
     1321 ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt
     1381 ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct
     1441 ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg ttatctactt
     1501 gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata
     1561 ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta
     1621 aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta
     1681 aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata
     1741 tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc
     1801 aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca
     1861 cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg
     1921 attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc
     1981 gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg
     2041 ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc
     2101 tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc
     2161 tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga
     2221 atccactact tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc
     2281 agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt
     2341 atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg
     2401 atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt
     2461 agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact
     2521 ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg
     2581 atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg
     2641 tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca
     2701 gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt
     2761 tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc
     2821 agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc
     2881 ttctttttct atttttgctt caagatcagt accctctttg cttacagatt gattttcagc
     2941 atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat
     3001 ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt
     3061 aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt
     3121 acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac
     3181 tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc
     3241 ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt
     3301 atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc
     3361 ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt
     3421 ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc
     3481 gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc
     3541 gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc
     3601 tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc
     3661 gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt
     3721 catcacttag cgatcggctc ctttactagg tttacgctac catttaatct atcatattct
     3781 atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt
     3841 cctatagaaa aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac
     3901 caaacagttt tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt
     3961 taattgtttt gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg
     4021 aatacgttcc cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc
     4081 atcatctaaa ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag
     4141 tacatcttcc aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc
     4201 ttcctggtct tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc
     4261 aagggaaaca ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc
     4321 catttccgca ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg
     4381 ttggatacgt atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg
     4441 tgcttggtca gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga
     4501 aaacttaaat cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc
     4561 ttcttggatc aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac
     4621 tactaaacga aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat
     4681 tttcaatgct aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt
     4741 cagatacata cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt
     4801 ttttgcttgt tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt
     4861 ttcatccacc acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc
     4921 tgcattcaaa gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg
     4981 tttgggtttg ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt
     5041 tccatttgcc ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc
     5101 gttaaattga tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg
     5161 gcttcttgct gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc
     5221 acatctagca agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg
     5281 atttccacca ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg
     5341 ttgaattcat aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca
     5401 tcatgaataa actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat
     5461 gcacgatata atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt
     5521 gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc
     5581 tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac
     5641 tctgttgaca gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt
     5701 tcaactagta attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct
     5761 tgttttaaat aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct
     5821 tggaaagatg cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg
     5881 actacgacag aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt
     5941 gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc
     6001 atttgttgga tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata
     6061 ccagcctgcc atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt
     6121 ttccggatat cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt
     6181 tctgtttctg gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca
     6241 agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact
     6301 ctttgataaa aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt
     6361 tctaacaatt catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca
     6421 ttaggtgcaa aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc
     6481 aaatattcta aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc
     6541 gctgcttttt catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct
     6601 atagtacgct gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc
     6661 actgcttctg gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct
     6721 ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta
     6781 cgttcttcat gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca
     6841 tattgtccaa tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga
     6901 ggaatccgtt gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga
     6961 agatatttca ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa
     7021 aggacacaat tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca
     7081 tttctatctc attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc
     7141 cttccagtac aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac
     7201 gctctctccc aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata
     7261 tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc
     7321 ttttacaact gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc
     7381 agcatttccc catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac
     7441 aagcaaggca tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt
     7501 tcttttatct tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat
     7561 cagcgtgatt tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga
     7621 taaaataaat gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt
     7681 gaccactcgg cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa
     7741 taccagattc gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc
     7801 tgcttctctt aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc
     7861 gctttctttc aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac
     7921 cgtatagcga tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac
     7981 atacgcatca taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt
     8041 gtctccagaa atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg
     8101 agcgtttttt cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg
     8161 tttttcttcg ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt
     8221 cgcttgggct gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc
     8281 tcctacgtac acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga
     8341 aaatgcgact ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc
     8401 ccgcatagtg atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat
     8461 ggctttctcg tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt
     8521 aatggtccct gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata
     8581 catgtcttga acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat
     8641 acttattagc aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat
     8701 aacagaaatt ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc
     8761 caaaggaata atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc
     8821 agaacgaata gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc
     8881 acctactcgg ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa
     8941 ttcagctcca gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc
     9001 ttctgcccgt ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt
     9061 taccaaatct cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat
     9121 cactagtact tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat
     9181 ttcttgataa attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga
     9241 atacgcacct acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt
     9301 caatatatta tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt
     9361 ttgctggcca gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt
     9421 tttctccatt ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa
     9481 gattaaaaac aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa
     9541 aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt
     9601 acttatattg ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa
     9661 gaacaggaca agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca
     9721 taatcgttga agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg
     9781 agaaaatgta atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga
     9841 tcggcacaat cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc
     9901 ccatatacac caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca
     9961 atccgccggc aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag
    10021 cgccgtataa taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag
    10081 aagtatctct tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac
    10141 atccgacaaa aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa
    10201 aagaaagtag aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat
    10261 tttctttgaa attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa
    10321 agacagctgc taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca
    10381 ttcccttcac aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca
    10441 ttttttgcct tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt
    10501 gaacatttcc tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc
    10561 attttgacaa tatcaggata aaacatgtta cctccattat gatagagata gccgccattg
    10621 taaagcaagg actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt
    10681 gggctcagca catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga
    10741 ttttttacat aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa
    10801 tcgcatcctt gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca
    10861 atactgtcag ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc
    10921 atgagatctg tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg
    10981 tagttttcca tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact
    11041 cgttttaccg aacgataagt tcttcctcct aaaaacaaca ccgcaccaat aaccgtcaga
    11101 atgatcatgt aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct
    11161 agttattttg acatcttttt ttatcttact atttctttat cttcttggct atttcttcct
    11221 tgatttctgt gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga
    11281 aaatgatcgt tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt
    11341 tacacagtag gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc
    11401 gaaatttact atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac
    11461 ggcagcggcc gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat
    11521 ctagtagata gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag
    11581 ttaatggccg aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt
    11641 cttggattta gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa
    11701 gttcatcgat tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc
    11761 agagtcatca gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt
    11821 cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat
    11881 gatttaaaaa aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag
    11941 ctaaaacatt ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa
    12001 gaacaaggac atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa
    12061 tttctttctg aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat
    12121 acaatggctg aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt
    12181 cattattgtc ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga
    12241 aacgtttgcc actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc
    12301 tgtgttacct atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa
    12361 tcctcgttac ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt
    12421 cggcgggttt gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag
    12481 aaacgtgatc caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta
    12541 cagtctgctt tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata
    12601 ttggatatgg ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt
    12661 caaaaagaaa ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt
    12721 ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga
    12781 aatccggatt gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat
    12841 cgtgtctatg attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc
    12901 cgctattggt gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta
    12961 ctacatcatt ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt
    13021 tgatcagcgt tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt
    13081 ggttgtctta ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc
    13141 ttttacagag tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca
    13201 atttcctagt atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg
    13261 tgtgaaacga gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt
    13321 attaagtgat tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct
    13381 tgcttctaaa aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat
    13441 cgatggaatc attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct
    13501 gccagatttc cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa
    13561 gatttggttt tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt
    13621 tcatttcctt caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct
    13681 gaagatcctt acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa
    13741 gcggatgttt gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt
    13801 caataataaa tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc
    13861 aatcgaagct tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac
    13921 aagcgaagag attgttatga ttttgcatga attcggctac gatttcatta agatggggga
    13981 agaagcttat gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg
    14041 tgctgtcttg aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt
    14101 ttctactgag caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa
    14161 ggaaaaaggg ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc
    14221 agttgtcaaa aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac
    14281 gaacaataaa gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg
    14341 cagtatggat ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg
    14401 gttcaattta gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca
    14461 ggaaaggatt gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt
    14521 aagagaatat aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag
    14581 agatagttgg attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga
    14641 gcagacacaa ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact
    14701 gcataccgat agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt
    14761 catgcttcta caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc
    14821 ctgtttggac cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt
    14881 tcttatgctt cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt
    14941 gagaatgtcg ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg
    15001 ttgtcaccag gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca
    15061 gttgtgcgga agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct
    15121 tcttcttttg tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct
    15181 ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg
    15241 cctacgttat ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt
    15301 acaactgctt tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga
    15361 acagcaccac gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg
    15421 tcttccactg gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca
    15481 tctactgtat ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct
    15541 tttaatgcgg aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat
    15601 aactcgcgaa cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact
    15661 ttgttcaaga aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca
    15721 cgtgtttgag gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt
    15781 gctgcccctg taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg
    15841 tagtggcgtt tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca
    15901 cgttcttctg gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg
    15961 cctaatactg tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc
    16021 gtaccaatgt taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa
    16081 cctcctaaat aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt
    16141 ttttgactaa atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt
    16201 attatacgtc aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa
    16261 aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa
    16321 ctttcttttg aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac
    16381 aactttcaat agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg
    16441 aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct
    16501 tatcattatt tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg
    16561 tcagctaccc aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct
    16621 tataataaag atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta
    16681 aagatgaatg cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa
    16741 gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg
    16801 gtcttaaaat tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
    16861 agaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
    16921 ctttactttt gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga
    16981 cacaattcat ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa
    17041 acgattcaat acttcatcca catttttgat catcacggat atcgtaccat ccggattatt
    17101 cacaaattca atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc
    17161 gtttgatagc ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt
    17221 tacaggaggt gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt
    17281 tgccgtgatg ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt
    17341 ttcttctatc gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt
    17401 ctcaaatttt tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa
    17461 agaaggtgca ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga
    17521 aaactcatat tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc
    17581 ttcatctgct ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc
    17641 gatccctgct tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa
    17701 taaagataag tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg
    17761 tgcttcttca ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc
    17821 cacaaaattt tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg
    17881 tccagttttt gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat
    17941 atattctttt gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc
    18001 aataatatgc agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt
    18061 agtttacgga aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat
    18121 ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga
    18181 acgaataaaa aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat
    18241 ggttacgaaa tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct
    18301 gtttcctctg ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga
    18361 tacaagaatg aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag
    18421 atattcttga caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct
    18481 tttgaagaat cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg
    18541 ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca
    18601 tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc
    18661 caccatctag atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc
    18721 cttgcaccag gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc
    18781 ctcgaagcat atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa
    18841 atcgctgaag cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac
    18901 gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta
    18961 tttgattttg aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc
    19021 ggcaaagcaa aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc
    19081 aatcgaacgc ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt
    19141 ctttttgatc gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca
    19201 aatcatcgaa aaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga
    19261 acatgaagtc atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa
    19321 a
//
LOCUS       DANKHK010000047        18976 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000047 DANKHK010000000
VERSION     DANKHK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(145..309)
                     /locus_tag="PW109_001539"
     CDS             complement(145..309)
                     /locus_tag="PW109_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511372.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            604..1932
                     /locus_tag="PW109_001540"
     CDS             604..1932
                     /locus_tag="PW109_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2511373.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2134..2967
                     /locus_tag="PW109_001541"
     CDS             2134..2967
                     /locus_tag="PW109_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2511374.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2983..3720
                     /locus_tag="PW109_001542"
     CDS             2983..3720
                     /locus_tag="PW109_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2511375.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3875..4843)
                     /locus_tag="PW109_001543"
     CDS             complement(3875..4843)
                     /locus_tag="PW109_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2511376.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5075..5908
                     /locus_tag="PW109_001544"
     CDS             5075..5908
                     /locus_tag="PW109_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2511377.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5942..6781
                     /locus_tag="PW109_001545"
     CDS             5942..6781
                     /locus_tag="PW109_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2511378.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6807..7367
                     /locus_tag="PW109_001546"
     CDS             6807..7367
                     /locus_tag="PW109_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2511379.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7566..9287
                     /locus_tag="PW109_001547"
     CDS             7566..9287
                     /locus_tag="PW109_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2511380.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9452..10594
                     /locus_tag="PW109_001548"
     CDS             9452..10594
                     /locus_tag="PW109_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2511381.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10610..11821
                     /gene="thiI"
                     /locus_tag="PW109_001549"
     CDS             10610..11821
                     /gene="thiI"
                     /locus_tag="PW109_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2511382.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12394..13041
                     /locus_tag="PW109_001550"
     CDS             12394..13041
                     /locus_tag="PW109_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2511383.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13170..13386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13434..16079
                     /locus_tag="PW109_001551"
     CDS             13434..16079
                     /locus_tag="PW109_001551"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2511384.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16389..17702
                     /locus_tag="PW109_001552"
     CDS             16389..17702
                     /locus_tag="PW109_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2511385.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17692..18345
                     /locus_tag="PW109_001553"
     CDS             17692..18345
                     /locus_tag="PW109_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2511386.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18400..18951
                     /gene="radC"
                     /locus_tag="PW109_001554"
                     /pseudo
     CDS             18400..18951
                     /gene="radC"
                     /locus_tag="PW109_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         6388 a         3206 c         4011 g         5371 t
ORIGIN      
        1 gccggagtaa ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc
       61 ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga
      121 aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata
      181 aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc
      241 aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag
      301 gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat
      361 tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat
      421 tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat
      481 ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga
      541 ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat
      601 tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat
      661 atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa
      721 aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa
      781 gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt
      841 cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt
      901 ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa
      961 gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc
     1021 caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg
     1081 ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg
     1141 cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt
     1201 gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga
     1261 acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac
     1321 cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat
     1381 gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag
     1441 atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact
     1501 ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat
     1561 gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat
     1621 gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt
     1681 ctgatgaccc atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa
     1741 tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt
     1801 gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa
     1861 gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt
     1921 gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct
     1981 taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata
     2041 attctaaata aaggataagg attggaaata atccttttat cattaaaaat atgatgacga
     2101 agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat
     2161 ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt
     2221 ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt
     2281 tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg
     2341 tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga
     2401 aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca
     2461 caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa
     2521 gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa
     2581 gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta
     2641 ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt
     2701 ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat
     2761 gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt
     2821 ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt
     2881 taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa
     2941 gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc
     3001 aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg
     3061 atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt
     3121 gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat ggagattaat
     3181 cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag
     3241 tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata
     3301 gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag
     3361 atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa
     3421 aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat
     3481 cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt
     3541 ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg
     3601 aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat
     3661 gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa
     3721 aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg
     3781 tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc
     3841 tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt
     3901 cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat
     3961 taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat
     4021 cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt
     4081 ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag
     4141 cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca
     4201 tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct
     4261 ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac
     4321 ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg
     4381 cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac
     4441 ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg
     4501 aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa
     4561 aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg
     4621 cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg
     4681 agggaagatt gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg
     4741 catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca
     4801 tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac
     4861 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     4921 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     4981 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     5041 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     5101 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     5161 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     5221 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     5281 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     5341 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     5401 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     5461 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     5521 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     5581 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     5641 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     5701 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     5761 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     5821 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     5881 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     5941 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     6001 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     6061 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     6121 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     6181 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     6241 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     6301 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     6361 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     6421 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     6481 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     6541 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     6601 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     6661 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     6721 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     6781 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     6841 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     6901 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     6961 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     7021 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     7081 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     7141 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     7201 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     7261 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     7321 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     7381 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     7441 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     7501 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     7561 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     7621 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     7681 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     7741 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     7801 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     7861 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     7921 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     7981 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     8041 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     8101 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     8161 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     8221 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     8281 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     8341 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     8401 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     8461 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     8521 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     8581 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     8641 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     8701 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     8761 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     8821 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     8881 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     8941 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     9001 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     9061 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     9121 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     9181 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     9241 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     9301 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     9361 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     9421 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     9481 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     9541 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     9601 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     9661 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     9721 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     9781 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     9841 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     9901 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     9961 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
    10021 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
    10081 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
    10141 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
    10201 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
    10261 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
    10321 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
    10381 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
    10441 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
    10501 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
    10561 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
    10621 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
    10681 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
    10741 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
    10801 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
    10861 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
    10921 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
    10981 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
    11041 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
    11101 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
    11161 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
    11221 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
    11281 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
    11341 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
    11401 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
    11461 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
    11521 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
    11581 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
    11641 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
    11701 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
    11761 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
    11821 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
    11881 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
    11941 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
    12001 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
    12061 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
    12121 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
    12181 ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    12241 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
    12301 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
    12361 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
    12421 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca
    12481 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
    12541 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
    12601 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
    12661 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
    12721 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
    12781 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
    12841 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
    12901 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
    12961 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
    13021 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
    13081 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
    13141 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
    13201 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
    13261 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
    13321 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
    13381 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
    13441 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
    13501 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
    13561 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
    13621 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
    13681 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
    13741 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
    13801 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
    13861 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
    13921 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
    13981 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
    14041 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
    14101 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
    14161 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
    14221 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
    14281 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
    14341 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
    14401 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
    14461 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
    14521 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
    14581 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
    14641 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
    14701 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
    14761 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
    14821 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
    14881 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
    14941 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
    15001 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
    15061 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
    15121 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
    15181 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
    15241 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
    15301 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
    15361 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
    15421 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
    15481 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
    15541 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
    15601 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
    15661 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
    15721 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
    15781 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
    15841 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
    15901 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
    15961 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
    16021 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
    16081 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
    16141 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
    16201 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
    16261 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
    16321 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
    16381 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
    16441 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
    16501 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
    16561 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
    16621 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
    16681 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
    16741 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
    16801 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
    16861 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
    16921 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
    16981 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
    17041 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
    17101 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
    17161 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
    17221 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
    17281 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
    17341 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
    17401 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
    17461 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
    17521 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
    17581 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
    17641 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
    17701 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
    17761 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
    17821 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
    17881 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
    17941 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
    18001 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
    18061 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
    18121 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
    18181 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
    18241 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
    18301 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
    18361 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
    18421 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
    18481 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
    18541 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
    18601 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
    18661 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
    18721 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
    18781 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
    18841 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
    18901 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgtttt aaccatagta
    18961 taataaataa tagtag
//
LOCUS       DANKHK010000048        18857 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000048 DANKHK010000000
VERSION     DANKHK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(91..1389)
                     /gene="eno"
                     /locus_tag="PW109_001555"
     CDS             complement(91..1389)
                     /gene="eno"
                     /locus_tag="PW109_001555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2511387.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1505..2260)
                     /locus_tag="PW109_001556"
     CDS             complement(1505..2260)
                     /locus_tag="PW109_001556"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2511388.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2364..3554)
                     /locus_tag="PW109_001557"
     CDS             complement(2364..3554)
                     /locus_tag="PW109_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2511389.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3664..4665)
                     /gene="gap"
                     /locus_tag="PW109_001558"
     CDS             complement(3664..4665)
                     /gene="gap"
                     /locus_tag="PW109_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2511390.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4706..5797)
                     /locus_tag="PW109_001559"
     CDS             complement(4706..5797)
                     /locus_tag="PW109_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2511391.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5946..7280)
                     /gene="rpoN"
                     /locus_tag="PW109_001560"
     CDS             complement(5946..7280)
                     /gene="rpoN"
                     /locus_tag="PW109_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2511392.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7340..7411
                     /locus_tag="PW109_001561"
     tRNA            7340..7411
                     /locus_tag="PW109_001561"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7373..7375,aa:Arg,seq:ccg)
     gene            complement(7603..8488)
                     /locus_tag="PW109_001562"
                     /pseudo
     CDS             complement(7603..8488)
                     /locus_tag="PW109_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(8575..9474)
                     /locus_tag="PW109_001563"
     CDS             complement(8575..9474)
                     /locus_tag="PW109_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2511393.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9491..9997)
                     /locus_tag="PW109_001564"
     CDS             complement(9491..9997)
                     /locus_tag="PW109_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2511394.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10186..10806)
                     /locus_tag="PW109_001565"
     CDS             complement(10186..10806)
                     /locus_tag="PW109_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2511395.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10882..11319)
                     /locus_tag="PW109_001566"
     CDS             complement(10882..11319)
                     /locus_tag="PW109_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2511396.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11482..12312
                     /locus_tag="PW109_001567"
     CDS             11482..12312
                     /locus_tag="PW109_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2511397.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12296..13663
                     /locus_tag="PW109_001568"
     CDS             12296..13663
                     /locus_tag="PW109_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2511398.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13682..14491
                     /gene="yidA"
                     /locus_tag="PW109_001569"
     CDS             13682..14491
                     /gene="yidA"
                     /locus_tag="PW109_001569"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2511399.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14534..14977)
                     /locus_tag="PW109_001570"
     CDS             complement(14534..14977)
                     /locus_tag="PW109_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2511400.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15111..15482
                     /locus_tag="PW109_001571"
     CDS             15111..15482
                     /locus_tag="PW109_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2511401.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            complement(15651..16432)
                     /locus_tag="PW109_001572"
                     /pseudo
     CDS             complement(15651..16432)
                     /locus_tag="PW109_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(16429..17400)
                     /locus_tag="PW109_001573"
     CDS             complement(16429..17400)
                     /locus_tag="PW109_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2511402.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17492..>18857
                     /locus_tag="PW109_001574"
     CDS             17492..>18857
                     /locus_tag="PW109_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2511403.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIV"
BASE COUNT         5301 a         3872 c         3370 g         6314 t
ORIGIN      
        1 tttcagcaag tagagagttc gattacttaa ctaagtgatc atactcgact atctttcaat
       61 agtcgatcat gtatcttcat tagaaaagaa ttatttgttt tttaagttgt agaaagattt
      121 caaacctttg tattctgcaa cttcaccaag ttggtcttcg atacgtagta attggttgta
      181 tttagcaata cggtcagtac gtgatagaga accagttttg atttggccag cgtttgttgc
      241 aacagcgata tcagagattg ttgaatcttc tgtttcacca gaacggtgag atacaactgc
      301 tgtgtagcca gcttctttag ccatttcgat agcttcgaaa gtttctgtta atgtaccgat
      361 ttggttcact ttgataagga ttgagttagc aattcctttt tcgatacctt cagataattt
      421 ttgagtgttt gttacgaaca agtcgtcacc aactaattga actttgtcgc ctaatacgtc
      481 agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat ccatcttcga ttgagatgat
      541 tggatattta gaaactaatt cttcgtagaa tttgatcatt tcgtcagttg ttttttcgcc
      601 ttcgcctgag tcagctagta cgtaaacacc tttttctttg tcgtagaatt ctgaagaagc
      661 agcatccata gcaagaacaa cgtctttacc aggtacatag ccagcttttt cgattgcttc
      721 gatgattact tcaaaacctt cttcgtttga accaaggttt ggagcaaatc caccttcgtc
      781 accaacagaa gttgctaatc cgcgagcttt caagattgaa gctaatgcgt ggaatacttc
      841 agcacccata cgtagagctt ctttgaatgt tggagcgcca acaggcatga tcatgaattc
      901 ttggaagtcg attgagttat cagcatgtga tccgccgttg atgatgttca tcattggtgt
      961 tggcaatact tttgtgttga atccgcctag gtagtggtat aaaggtactt ctaggtagtc
     1021 agcagctgca cgtgcaacag cgatagaaac acctaaaata gcgttcgcac ctaatttacc
     1081 tttgttagga gttccgtcta aagcgatcat agctttgtcg atagccattt ggtcacgaac
     1141 atcgtagcca atgattgctt cagcaatgat gttgtttacg ttgtcgacag ctttagttac
     1201 acctttaccg ccataacgag ctttgtctcc gtcacgtaat tcaactgctt cgtattcacc
     1261 agttgaagca cctgaaggaa ccattccgcg gccaaaagct ccgctttctg tgtatacttc
     1321 tacttcgatt gttgggttac cacgtgagtc taagatctcg cgagcgtaaa catcagtaat
     1381 aattgacatg ttttgtctct cctttgagtt tgttttttcc taagggattt ccctcatata
     1441 cgattttagt taattttgct cattagtgca aacatttctt gcatttttca caaattttca
     1501 acaattattt tacagcgtct aacaatgaca agaatgaatc tgcttgcaag cttgcgccac
     1561 cgactaaagc accgtcaaca ttttcttttg ccatgtattc agcaatattt tctggtttta
     1621 cagaaccgcc gtattggata cgaactgctt ctgatacttc ttttccataa agtttttcaa
     1681 ctgtttgacg tacaactcca caaatttcgt cagcgatttg tgcgtcagct gatttaccag
     1741 taccgattgc ccagattggt tcataagcaa taaccatgtt tgacacttgt tcagctgaaa
     1801 gatcagctaa accagctgta atttgtcctt cgatccattc agcagttttg cctgcttcat
     1861 aggtttctaa agattcgccg cagcatagga ttggtgtcat accatttgca aagatagctt
     1921 ttgctttttt attgatgtct tcgtctgttt catggaaata ttcacgacgt tctgaatgtc
     1981 cgatgatcac ataatgaaca cctagatcag caagagctgc tggtgaagtt tcaccagtga
     2041 acgcacctga gttttcccag tagcagtttt gtgctgagat ttttagttct gtaccttcag
     2101 ctgatgtcat taattcttgt aagaataatg caggagatcc gatgactgaa tcaactttgt
     2161 catttgaagg gattttgttt tttactgctt cagcaaattc ttttgcttct gatgcagttt
     2221 tgttcatttt ccagttacct gcgataatag gtttacgcat tgaggaacac actccttaat
     2281 ttattgaaaa atggctaggg agtcatgaaa actcccttca ccttttgatc gattactcgt
     2341 cagaattgag caaacttggt cgtttattta tcgttgattg ctgctagtcc tggtaattct
     2401 ttaccttcaa gcaattccaa gcttgcgcca ccacctgttg agatgtgtgt gaatttgtca
     2461 gcgaatccta attgttctgc tgctgcagct gagtctccgc caccgatgat tgttgttgcg
     2521 ccttctaagt tagcgattgc ttcacaaaca ccgattgtac ctttagcaaa gttactcatt
     2581 tcaaatacac ccataggtcc gttccaaaca actgtttttg caccttgtaa tgtatcagca
     2641 aataatttta ctgatgctgg gccgatgtct aaagccatca aaccatcagg aatgtctcct
     2701 tcgattactt gtgtttcaac atcatttgaa aattctggtg cacagacatt gtcgattggc
     2761 aagacaagtt tgtcgcctgc tttttcgatt aattcttttg ctagttctac tttatcagct
     2821 tctactagag agttaccgat cttgattcct tttgcttcgt agaatgtgta agtcattccg
     2881 ccaccgatta agattttatc tgctttagga atcaagtttt cgattacgcc gattttatca
     2941 gaaacttttg cgccaccaag gattgcaatc attgggcgtt ttggttcttc tactgcttca
     3001 ccgatgaact tgatttcttt ttccatcaag aatccagcaa ctgttgggat accagttgaa
     3061 gcgataccta cgttagaagc atgtgcacgg tgagctgtac cgaaagcatc gttaacaaat
     3121 acatcgccta atgaagccca gtatttacct agttctgtat catttttgct ttctttttta
     3181 ccgtcaatat cttcaaaacg tgtattttcg aatacgacaa cgtcgccgtc attcatgttg
     3241 cggattgctt cttctaattg ttctccgcga gtttcaggta cgaatgttac ttctttacct
     3301 aataattcgc ctagacgttt tgcaactgga gctaaagatt ttccttcttt atcttcttct
     3361 gtttttacac gtccaaggtg agagaaaagg attgcttttc cgccttgttc tagaacgtag
     3421 ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag tgatcacgcc gtctttcaaa
     3481 ggaacgttga agtcaacacg gacaagaact tttttgtctt ttaaatcgat atctttcact
     3541 gttttcttag ccattttatt tcctcctgaa taaacttatt atcaaaaaaa gcggaagaag
     3601 cgcgattgct tccccgcttt taaatgtaca tgtctataat ttacacttgt acgtttgtct
     3661 atcttataag ttagcgaagt attctaaagt acgtactaat tgtgcagtgt atgacatttc
     3721 gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg ccaactgtca ttactttagt
     3781 ttgtgtagca tcaaataatg aaccgtaagt cataccaacg atatctgagg aaacgatttc
     3841 gtctgtgttg tatccgtaag attcgtttga agcttctttc attgctgcat tgatttcgtc
     3901 aacagttact tctttttcaa gaactgttac taattctgtt aatgaacctg ttggtacagg
     3961 aacacgttga gcagctccgt ctaatttacc gttcaattcg ggaattacta aaccgatagc
     4021 tttagcagca cctgttgagt taggaacgat gtttgcagca gcagcgcgtg cacggcggaa
     4081 gtcaccttta ggatgaggtc cgtctagagt catttggtca cctgtgtaag cgtggatagt
     4141 tgtcattaat ccttcaacaa caccaaattt gtcgttcaat gttttagcca taggagctaa
     4201 acagttagta gtacatgaag cacctgagat aactgtttct ttaccagtta atgtttcgtg
     4261 gtttgtgtta taaacgattg ttggaacatc gtttccgcca ggagcagaga taacaacgcg
     4321 tttagcacca gctgttaaat gtttttcagc tgctgattta gaagtgaaga aaccagtaca
     4381 ttctagaacg atatctacgc ctagttcgcc ccatggtaat tcttctgggt tgcggttagc
     4441 taatacttta acttcttttc cgtttacgtt gaatgaacct tcatgaactt caactgttcc
     4501 gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt gccaacattt tagcatctgt
     4561 caagtcgttg attgctacta cttcgattcc atctacatct tggatacgac ggaatgctag
     4621 acgtccaata cgtccaaaac cattaatacc tactttaact gtcatttaag atttcctcct
     4681 tatgaaaatc aaaaaaatat ttattttaaa gggttacccc ttttaaaatc tcatttgcag
     4741 cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt tttcatataa gctcgaattg
     4801 ctttagcttt tgattttccg cccgcgattg ccatgatgat cgggactttt tccaaatctt
     4861 gcaattctat gccgattcta gggaccttat aaacgacttc acctttttca ttgaaaaagt
     4921 agccaaaaga ttccgcaaca gcatttgctt gtttcagcat gactaattct ttttcagtca
     4981 tcttacgacg tgctgccatg tgtaatgcac gcccaatact atgaataaca caattcgctt
     5041 cactaatcaa gtttaagact tcttggatag atggctcatt gagtaaagaa ttgtaagttg
     5101 ccagacttaa ttgttccgga acataaagtg cgcggtggtt accgtttgct ttagcagcca
     5161 ttcttgcact gacagaattt gcctgtacag ttatcgcttc accaatcccg cctcttgcag
     5221 ggacaaacag gttatgccgt ttcttatttt ccagattaga caattgttcg gctaccacag
     5281 ccatagtagt tcctcccatc acagcgatga tattctcgcc ttctggaagt agtcggtcta
     5341 aggcttcgct cagcgcttcg ccgaatgcat cagccacttt ggtctgctct tcgctgtttc
     5401 ctgaaacaac gatacagcgt tggttaccaa aatattctgc caactgttgt tcggtttgat
     5461 gcgtacctaa aagctgatcc atgaagtttt caaggctttg ataaacttct tcgcccttag
     5521 ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt cagatttttc aaaaggtctg
     5581 tttctgttcg taagactcgt tctgtgatcc ccatactttc cgataaactt cgacgcccaa
     5641 tcggctgcat ccaatagata tttcgtaaaa ttttatagcg ttcttgcaac acttcgataa
     5701 tatctggtgc aacagattca atcattttta tttcatccag catagcgatc tccttagttg
     5761 gacactttac gtccaatgta gacttaaaac gacccatata cgataaaaaa agaaattaac
     5821 tattcgaatt tcacatcaat aaagggaaat ctcaacctca cgactgtaag tctaacagtt
     5881 ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa atactttttc tagtcttttg
     5941 actgattagt tatcataacg ttttcttttt gatgaactcg ggattcctaa taaatcacga
     6001 tattttgcta ctgttcgtct tgagatcgct atctcatctt ttttcagcaa ttcaaccaat
     6061 ttctggtccg acaacggctt gtttttgtct tcttgatcga ctaattcctg cagctttttc
     6121 ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac tattcgtagg aattcgtgta
     6181 gtgaaaaatt tttttaattc aaatacaccg aaatctgttt ccaaatattt cccgttgact
     6241 gcacgactga ccgtggattc atgcacatct atctcagttg caacttcctt taacgtaaga
     6301 ggcttgattg gacgttcctt gtcgataaag aaagcttttt gccgggaaac aatgacttga
     6361 gcaacccgca aaattgtatc cccacgttgg aggatcgttt tcttcaacca ttcgaattcc
     6421 tgtttccgtt cttttaaata attttcagtt tctttatctg cttgttgtga catttgtttg
     6481 aagtaactct cttgaaagcg tatatttggc tgattttttc ggtttgagat aacttgtaca
     6541 tttttatcgt cgtccactaa tacccggaca tcaggaataa tgtacaattc gctgctacgg
     6601 tcgaaaatcc ggccagggct tggactcaac gtttgtaaat agtcgaatat ctgctgaaca
     6661 gcatgtagat caacagaaaa acgttgtgcg atttctttcc attttcgctc caccagttcc
     6721 tcaaaacatt cttccaacac gatataagct aattccggcg cataatcatc tcgttctgtt
     6781 tgaagcatga gacattcttg caaactacgt gcacctactc cagctggttc taattgctga
     6841 atcagagtca acgcatcgag catttggata ggcgtagctc caatctcttt tccggcttct
     6901 tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt ctacaagata aagaacgatt
     6961 ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt cgatcaatga atcaaataaa
     7021 gacccttttg tatcaggaat ctgatttagg taatttgtct cttctccttc ataagaagat
     7081 gaagaagctt ttatgtaatc tggctgccat tccggctcaa caacttccac caatggattt
     7141 tccattgctt gattctcaac gaatgccaac agctcctcac tgttatattg taaaacttgg
     7201 atggactgtt gcaactgttg cgtcatggct agtttctgtg tctgctgctg tttttgtgaa
     7261 aaattttgct caaatttcat ttttttgtcc aatccccttg taatttttta tctgtttagt
     7321 tacaattatc ccagtgcttg cgtccttagt gtagtggata tcacgtaaga ttccggttct
     7381 tgagacgggg gttcgattcc ctcaggacgc gttctactgc tattttagca taatttacga
     7441 atagttgctt ctttattttt aagtgaaaac tgtccgccaa agagaattgg tggacagttt
     7501 tttgttgtat ctgtatagat attgttaagc ctttgcaagt aaggtagtta tgtgttcaac
     7561 tacttattaa aatgtgttat ttattaaaga tacttgtctc acttactgaa ctggccgatg
     7621 atcgtttctg ccataatagt tctcgacagc cgttggatgc cctaataaat cgctaactgt
     7681 cgtgatgcga tagccttgct ctttcagata atcgatcact tgaggaacgg cacgaatcgt
     7741 ttcaggatag atatcatgga ataaaataat tgacccatcg tatactgttg cttggatctt
     7801 ctgcaaaatc aaatcaggat tccttgtctt ccaatcttct gaatcaacag accattcgat
     7861 gatcggtcgc tggatgatcg ttgctcctac attcgtgaca gaaccatagg gcggacgaac
     7921 aaaagcgggg gttttgccag tgactttttt tgatctcagt gtctgctttc gtcacttcct
     7981 ccgtcatcga ctgggcagaa agagtggcca gattctgatg atcgtatgtg tgattgccaa
     8041 tctcgtgccc tgcttcagct gtctgcttca cgatttcagg atgctgcgcc gtattttctc
     8101 ctaagacgaa aaaagtgaca tgcacgtttt tttcttttaa gattcgcaga agctgcggcg
     8161 ttgtggtcgc tcctggtcca tcatcaaagc tgattgcgac gcttttgaca gtcgggtctt
     8221 ggattgcttc tgtactgtct tgtgttgatt gtgatgattg actttgtacc ttttccgctg
     8281 attcttgaat ggcttctttg acgttagctg tttcagttga ttggctttta atggttgatt
     8341 gttctgattg tttgaaagat tgtgacgaat gctccgaaga gtgtgtttgt ccttttactt
     8401 cggtgatcaa ggaggccacc gaagtaattt cgaaaaacaa aagcacggat aaagcaataa
     8461 tactgatgac taaataattt cttttcacta tatctcttct ttctcttttt ctcttcttat
     8521 tatttacttg tacttacttg aaaattgaaa ctgctagtcg caactacttc tcgtctaatg
     8581 atccaaataa tcttccgatg ccaaaatcgt ttttgtcacg gacttaggaa tgacaaattc
     8641 tggtgtaccc atataaccag gagcaatttc gtattgagaa aaagtcaaaa ctaattgatg
     8701 ctctttgttc aaataaaaac ttcgtgtagg cgtaacaagt gttggttttt ctatcgtccc
     8761 ttcttgaata tcttgctcag tccaatagta cttagatgga tcttccttga cttgttcggc
     8821 catctgccgt ttgacttctt ttgtcaaaac agatagataa gtgtcatctt tgaataaaag
     8881 tggaagtgag agctggacac tgtttttctt gtcgattgtg tcgtatcttt tttcaacatg
     8941 gctatctgct gctgtttggg tgatctgcct ttcaatcacg agaaaccggt tatcgtctac
     9001 gattttctga taatctccca aaacttggta atgatccgtt tcaaaatttc catattcttt
     9061 ttcagctttt tcaaaatctg cttgtccttc gcgaaagtat ttttgattta atccatgaat
     9121 tgttttattt tctttctcat tttgtgtatt gatcttcggt acctgaatat cgattttttc
     9181 agatcgatca gtaaagcgtt cgcctaagat caattcaatg actgatccta agatgggtaa
     9241 atcttctgca aagcttcgaa cttgactatt aaaaagaagg ctacttgttg taatcattac
     9301 gatagccaaa gatactccga acccttggag acgtctcttt ctccgccttg cttttataaa
     9361 tgattgctct tcgataataa aacgctggcg gatctcctct ttaagccctt tgggaattgc
     9421 ttgttcttta taagttttct ttagtttttt taaattttct ttttgggaat acatggcact
     9481 cttctctcct tcattctagg atttgacgta aagcattcaa tgagcggtac attttcgttt
     9541 taactgtgtt ttcattcata tccaagatag ttgcaatttc tttcaaacga aatccttcaa
     9601 aaaagtagag gatcagaatc tctttttccg gactgtctat tttatctaaa atcatatgga
     9661 gctctgcaac ggaagtttct aaaggtaact gttgggggat atcttgaatt tcaaataagt
     9721 ccacaggcgt ccttttttgt ctgcgccaac tatctaatgc cgtgcggatc aatatccgat
     9781 aaaaccatgc actaaaatgc tcgatctctt tttctttttc aaaatagttc aaggctttat
     9841 gaaaactatc ttgcatcaca tctaaggcct cttctttatc atgtacgtaa ttcatcgtga
     9901 tacggaacat tttttgataa tcagtttcga tgagtccatt cagcaggtga cggtattttt
     9961 cgatctgccg ttttttctta tatccagtaa aaatcattct ccactccttt ctgcctcact
    10021 taatatgacg tacaaggtgc gctaaaagtt tcaatttttt tattttttat gtatttactt
    10081 gggattttta tatacaaaaa agaaggtgtg acaaaaatcg tgtcacacct ccttattctg
    10141 ataggctgat cgtctctgtc acaacctctc gtaagaaaat aatgtttatt ctttttcaga
    10201 gtcagaaaat tcttcctctt cataatcttc atcatcatcg tcttcgtcta tgatacttaa
    10261 atcttcttcg atatttcctg gcaagtcttc ttcatcatct gtcgggtcat ctgctccgat
    10321 ttcttgcaag tcagaattat aagcagcgat ttcttcatct tcatcatcat cgtcatcaaa
    10381 cagatcatcg tcatcttctt ctgtcagatc tgtatcttct gggtcatcgt cattgtaatc
    10441 gatcgcgtct tcatcatcat tgataaaggc gttgactttc ttgcgtttac ggcgacgtgg
    10501 agtatcttca tcctcttcgt ccattccatg gtttacttct tcatcgatcg aatcgatagc
    10561 ataccatgaa cgcaagcccc aacgattatc ccctaatgag ataaagctgc cgtcaatatt
    10621 caagtcagta taaaattgtg cgagtgaatc acgaatttca ctgtctgatt tctcaagata
    10681 gttttggatc tggttagcta aatcagaaaa atccatgaca tcgccacgtt gttctaagat
    10741 cgcgtgtgct acttcgatca ttgataattc gcttttgttt gcaccttcaa atacactaat
    10801 ttccaatcag gacacgtcct ttcacagtct actccttatc atacaaaatc agacacaaaa
    10861 aatcaaactt ttcttttata tttacgctgt aaaatccaag gtcaaataat attcgccgat
    10921 tttttccggt cccatgaaca gatcataatc gattcgaacc ttccctgctg tgggacggtc
    10981 tttcaaacta acatgcagtt ctttcgtaaa agtagcaatc tggatcatac cgtatggtgt
    11041 tcgataggat gtttctaacc gttctttata gccaaatttc aaacgcatcc gcatttctcc
    11101 tgcacggatc agctgtacat gaccatctgg aactaatttg atcgttacag gaatctcatc
    11161 gcccgtctcc tcctgaatct ctttataacg tatgtacagc gtatcgccaa ttgtcaccat
    11221 ttgccctttc aaatcaaaat aaaaatcttg cacttcccct tgctgtttta cttttgtacg
    11281 caatttgatt gaaatcggtg ttccttgtga taattccatt attcattctc ctttacttct
    11341 catagctaca ttgttcctag tttcttttgt tttgtcaacc aacttgtcca tttttacaga
    11401 aaaccttttt tccaaaacaa ctctttgatt ttagagtata atattgtaga aaatatgaat
    11461 ttatacaaag gagccggttg aatgaaccct gcgattcgac tagatcagca catttatcaa
    11521 aaaaacgact tttccccttt tgcattaaca gatatattaa cggaatattc taaagaacac
    11581 cagcaaatct ttttacattt ctggcaatat gactgcacag tcatcttagg tatgaaggat
    11641 gttcgaactc cttttctaag tgacgggatc caaacactga aagatgcagg ttacgctcca
    11701 gtgatcagaa attcaggtgg attagggatc gtatcagatg aaggaatttt aaatatttct
    11761 ttgattttcc ctcaaagtga cacaaaaaaa ataaccatag atgacgctta cgagcaaatg
    11821 ctcctattga ctaaatacgc ttttccaaag gcaccgatac aagcattcga aatcacccat
    11881 tcttattgtc ctggaactta tgatttaagc atccatggca aaaaatttgc cggtatcgct
    11941 cagcgtcgga tcaaaaatgg gatttctgtt atgatgtact taagtgtcaa cggtaatcag
    12001 gaaaaacgcg ggcaagtcat gaagcgattt tatcaatctg ctttaaaaga agactttggt
    12061 acaaatggct atcctgcagt ggatcctgct acaatggcaa cacttgaaga actgcttgac
    12121 caaccccttt ttgtaaaaga agtcaaagaa gcatttgcaa aagcttttga aaagctgtat
    12181 tctgcaagtg atttacagga ttctaccgac tggctaaaaa aagaaatcca gctggagcaa
    12241 tggaacaacc aaatccagag aatggaacaa cgtaataaca taaaggagct acattatgac
    12301 aaccccttat aaatatcaaa tgctgcctat ggaaaaagtt tttcgtgatc cagtccataa
    12361 ctatatccat gtccagcata aagtaatctt agatttgatc aattcgaaag aagtccaacg
    12421 tttacgacgc attaaacaat taggcacttc ttctttcact tttcacggag cagaacatag
    12481 ccgttttacc cattcattag gcgtttatga gatttcaaga cgaatctgcg atatcttttc
    12541 tcgaaatttt tcaaaagaaa aaataggcaa cggaggctgg gatgatcagg aacggctggt
    12601 gactttatgt gcggcactgt tgcatgatgt cggtcatggt ccttactctc atacatttga
    12661 acatatcttc catacgaatc atgaagccat cactgtagag atcatcactt ctccagaaac
    12721 agaagtgtat cagattctta accgagtaga agaaggtttt cctgaaaagg tagctagtgt
    12781 gatccaaaaa acttacccca acccacaagt tgtccaaatg atttctagtc agatcgatgc
    12841 agatcgaatg gactatttgc ttcgggatgc ttatttcact ggaacagaat atggaacatt
    12901 tgatcttacc cgaatattac gtgtgattcg tccttatgaa ggcgggatcg ctttttccat
    12961 gagtggcatg catgcagtgg aagattatat cgttagccga tatcaaatgt atgtgcaagt
    13021 gtacttccat cctgtttccc gtggtatgga agtgattttg gagcatctgc ttcatcgcgc
    13081 acacgagttg tatcaagaag atgccagcag ttttgaactc cattctcaat tacttctgcc
    13141 atttcttgac ggaagcttta ctttagaaga atacctcaaa cttgatgatg gggtcttggg
    13201 cacttacttt actcaatggt ctgaatctgc tgatcctatt ttaaatgatt tagctaaacg
    13261 ttttttgaat cgtaaaccac tgaaatctgc tactttttct ggcaaccggg attcaaaatt
    13321 agtccaagaa ctcactttgt tagtagaaaa ggttggctat aaccctgttt attacactgc
    13381 tgtgaattct agctacgatc tgccttatga tttttatcgc ccagaacaag gcagacatcg
    13441 cacacaaatc gagattttgc gaaatgacgg aactttgata gaattatcac aggttagtca
    13501 gcttgttgct gcattagcgg gacaagaaca aggagatgag cgatttttct ttccaaaaga
    13561 aatggttgat ccctccttgc gtgatcatta tgatttattt gacgaaactt atcaagaatt
    13621 tgccagccat atccgtaatg gcgcattgat tgaaataaat taaaatatat tggaggaaat
    13681 tatgtctatc aaactagtcg ctatcgacat cgatggtact ttattaaatg agaaaagaga
    13741 agtaacaaaa gaagtaaaag aagcgattgc cgctgctgtt gcaaaaggtg tttcgatcgt
    13801 tttatgcact ggccgtccct taccaggtgt ccaggaacag ttaaacgagt tgaacctttt
    13861 tcaagataat gattacgtta ttacttataa cggggcactc gtacaacaaa cgaaatcagg
    13921 taaaatcatc gcccgtcacg gactgactca tgaagatttc ctagagatcg aagtgatggc
    13981 acgccgtgtc ggcagtcatc tccattcgat cgatgacaaa gcgatctaca cgcctaatcg
    14041 agatatcagc gcttatacga tccacgaagc aagtttagtc aagatgccgc taaaatatcg
    14101 aacacctgaa gaaatgacac cagacatgaa catcgtcaaa atgatgatga tcgacgaacc
    14161 agaaatttta gatgcagcaa ttgcgcgttt accgcaaact ttccgtgata aatacacaac
    14221 ggtcaaaagt gcgccctatt acttcgaagt actaaataaa gaagcaagta aaggtgcggc
    14281 agttgcgaat ctcgcacagc accttggtat tgatcaagat gaaatcatgg ctatcggaga
    14341 taacgagaac gacttatcca tgattgaata tgctggtcta ggagtagcaa tgggaaatgc
    14401 cgtttctctt gtcaaagaag cagcaaatgt catcacttca tcaaacgacg agcacggtgt
    14461 agctgaagca atcaaaaaat atgtactgta ataaataaaa aaactgagag aaagctgatc
    14521 tactcgcttt ctttcagttt ttattttttt cgatccatct ctcgactgcc catcggtggc
    14581 taccacgttt caatttcaca agcgcttctt gagggctcac ccaccaaatt ttattggtct
    14641 tttcagttgg ttctcctata cgcttccaag aatcagcaac aaagaaatat cctggattat
    14701 aataataagt tgcacgataa ttcgagtaaa aatactcatc agcttctccc agataagaag
    14761 aaatttctgc cacaattccc atttcttcta tcagctcacg gacaatcgct tcttctttcg
    14821 tctcttctcc ttcgatttcc ccaccaggaa ggaaaaaagc accatttgga gcctcgatca
    14881 ccgcaatccg cttgtctctt tctatgacaa tatatgctgc ataacgggtt tgataattag
    14941 cagattgctt tttttcacca aaaattggtt tatccatgaa ttgtttccac cttaacagta
    15001 ttattttcta tccattttac atgagaaatt tctcatgaac aagcacaaat caactatttt
    15061 cttacttctt tttgttatga ttgagtaaaa ggaaaaggag agatgaattt atgaaaatgg
    15121 ctcacacatg tgttcgggtg aaagatcttg aagcatcaat cgaattttat caaaaggcat
    15181 ttggttttga agaaagccgc cgccgtgatt ttccagaaaa caaatttaca cttgtttatt
    15241 tgacacttcc tggagatgac tatgaattag agttgactta caactatgat catggtgcgt
    15301 atgatctagg agacggctat ggtcatatcg ctattgcagc tgatgattta gaaaaattac
    15361 acgagcaaca taaagctgca ggtcttgaga ttactgactt aaaaggatta cctggcacta
    15421 cgccttctta ctactttgtc attgaccctg atggttataa aatcgaagtc atccgaggat
    15481 agttagtatg gggcatttta ctcttgattg aataaaaaac aaacaccaat aattaaaaaa
    15541 agacgcaaac ttttcattaa attgaaaaag tttgcgtctt tttttaacaa ccattgtagc
    15601 tataaggctg ccactctcca ccaatttatt tttaattcat ttgaaagaaa tcaaataaac
    15661 gaggtacctt tagaaaacaa aactgctttt cctcctatta tcacacgatc ttcttttaat
    15721 tcacattcta gatccccgcc ccttgatgac atttgataag ctgacaattt tgttttattc
    15781 attctttctg cccaataagg aataagatta gaatgtgctg atccacatac tggatcttca
    15841 ttgattgtta attttggaaa gaaggttcta gaaacaaagt ctttttgcac actttctgct
    15901 gtcacgatga ctctattcct aattcaaaat ttttgatagc tgtaaaatct ggctgcaatt
    15961 tagccaccgt tttttcatct tctaatacga aaaataaatc acgtgctaag taggcttctt
    16021 taatcttagc cccaattgct tcttcatatt cagtcaatat cggcactttt ttcggcataa
    16081 tactaggaaa atccatgtaa taataatctt taattcttgt cacaaataga ttaccgcttt
    16141 gagtagaaaa tttaatcgtt tcatcaggta ttttgtaata gttaaataaa acaaatgctg
    16201 ttgctaatgt cgcgtgacca caaagatcga tttctcgatc tggcgtaaac catctaagct
    16261 caaattcttg gtttttcttc actgtaaatg ctgtttcaga taaattgttt tcaatggcaa
    16321 ttttttgcat cgtaccttct ggcagccatt cttctagcac ataaaccgct gctggatttc
    16381 ctttgaacac ctcatctgca aatgcatcaa ctacgtaata atctaagttc attttaaagc
    16441 ccctttctct aatttgttat aaatatattc tgcacaacaa agatcttcaa tagctagacc
    16501 aactgcgtcg aaaatcgtcg gcctcgattc tcctttataa aatgtttttt tctcagaaac
    16561 taattcgcct aatgtaccaa caatacaaga ttcctctaaa tctccttctg ccatggctat
    16621 aagcaggtcc ccgctttctg ttaaggtagc tgagtaatca tctacataga tttgagaagt
    16681 ttccatcaac gatgtactta tctctcttgt atcaggagta aatgtgccca cagcattgat
    16741 atgtgcgtct tcccttaccc actctttttg aagaaatgcc tctttactag aggtcaacgt
    16801 acaaatgatt tccgctggac gtactgcatc ttctactgat attgcattta taaacgttat
    16861 tttaggatac tgttttttag ctttagcgat aaaagtattt cgtctttctt ctaaaatatc
    16921 gtaaacaaac actgtagtga tgggacgaat ggtagaaatc gcagtcaaat gcgacaatgc
    16981 ttgttgacca gctccaatta aagcaagtgt ttctgcatgc ggatttgcta agtaatctgt
    17041 tgccagcgca gatacagcag ctgttcgaat ccaggtgact gcatttgcgt ttatcaatgc
    17101 tttgggtagt ccgttttgag catcaaacaa gaaaatctct cctaaatgag aaggtaaatg
    17161 atactgatga ttatccggaa aagcggtaat gacttttgtt ccaaaatatc tgttctcccc
    17221 caaataagca ggcatcatcg caaacatatt tttgtctttt ccgtcaggta aaatttccac
    17281 caatcgagga ttttggctga ttttaccact ttcataatca aaaaaacatt ttttcattac
    17341 ttgaatcgct tcttcaaaac taaaagaagc tactacttcc tcaaaattaa taattttcat
    17401 agacaattcc ctcctcacaa aagattaact tctaaaatca atacaatcaa tggtaaaatt
    17461 gtatggtata caattttaag gagtggagaa aatgcctatt aattcttatg acaattaccc
    17521 gttaacttgg cgaccagaca aacaaaaatt gaagcgtcct tattatcgat cgttgattca
    17581 acaattagaa caagatattt taaagaataa attacagaaa aacactcagt taccctccca
    17641 aagagagcta gctgattatt tagatttaaa ttttactacc gttggcaagg cttataaata
    17701 tggaattgag aaaggtttgc tgtatacgaa tattggaagt ggcacattta tttctcccaa
    17761 tgtctttgag tccatcacca tctctaccaa tgaagtaacc gaacatatca ttgattttgg
    17821 tctggttagt tcttttgaag aatgtaataa attcgttgtc ccgtttattc aatcaactag
    17881 taataaccca aatcttgttg gtttgcttaa ttaccaagat cccaaaggca caaattttca
    17941 attgactact gcagcaaatt ggttaaaaac tcaaggagtc attccgtcct tagaatctct
    18001 atctattgtt tccggtgttc aaaatggatt ggctatcaca ctttctgcac ttttctctcc
    18061 aggtgataga atagcaacag accgatatac atacgctaat ttcattgaat tggctcagtt
    18121 atatcattta gagatcgttc caattgattt tgacgagcaa ggaatgcgac ctgatatatt
    18181 gattcaggaa tgtagaaaaa agaagattca tggtgtcttt cttatgcctt cttgtaataa
    18241 tcctataggg tttcaaatct ctcacaaaag acgagagcaa ttagccgaag tgattgaaaa
    18301 agaaggtcta tgggttattg aagatgatat ccattcattt ttaacaactt actatcaaca
    18361 tacgatcatt cctcctttcc aaaaattatt acctaaccaa acaatctact tagctggaat
    18421 gacaaaattt atttgttctg gattgcgagt tgcttatctt gtagttccag tacatgtcca
    18481 aaacaagata gaacaagcaa tttttaatat aaatgtgaaa acttctggat tagatgtgga
    18541 agtcatcact caaatcctcc attcaaatga agcaaatagg atcttagaaa ctaaattaag
    18601 tcttacgaaa agagccaatc agctttttga ttctcttttc aaagtagaaa aaccaacgaa
    18661 tcgtgtccct tactatcgaa ttcttccagt tgatcctgag attgatccta agataattga
    18721 gaatgacttt cttgctaacg gtatccgact ttatcattcc agacgcttta ctacacgtgc
    18781 gcaaccagat gcattcgttc gaatttccct atcctctaat aaccttgaaa ttttagcttc
    18841 tggactaagt atcgtaa
//
LOCUS       DANKHK010000049        18141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000049 DANKHK010000000
VERSION     DANKHK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            227..1009
                     /gene="rpsB"
                     /locus_tag="PW109_001575"
     CDS             227..1009
                     /gene="rpsB"
                     /locus_tag="PW109_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2511404.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1108..1989
                     /locus_tag="PW109_001576"
     CDS             1108..1989
                     /locus_tag="PW109_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2511405.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2125..2847
                     /locus_tag="PW109_001577"
     CDS             2125..2847
                     /locus_tag="PW109_001577"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2511406.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2850..3407
                     /gene="frr"
                     /locus_tag="PW109_001578"
     CDS             2850..3407
                     /gene="frr"
                     /locus_tag="PW109_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2511407.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            3602..4414
                     /locus_tag="PW109_001579"
     CDS             3602..4414
                     /locus_tag="PW109_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2511408.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            4411..5211
                     /locus_tag="PW109_001580"
     CDS             4411..5211
                     /locus_tag="PW109_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2511409.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            5372..6640
                     /gene="rseP"
                     /locus_tag="PW109_001581"
     CDS             5372..6640
                     /gene="rseP"
                     /locus_tag="PW109_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2511410.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            6708..8417
                     /locus_tag="PW109_001582"
     CDS             6708..8417
                     /locus_tag="PW109_001582"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2511411.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            8629..12981
                     /locus_tag="PW109_001583"
     CDS             8629..12981
                     /locus_tag="PW109_001583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2511412.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            13125..13598
                     /gene="rimP"
                     /locus_tag="PW109_001584"
     CDS             13125..13598
                     /gene="rimP"
                     /locus_tag="PW109_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2511413.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            13622..14797
                     /gene="nusA"
                     /locus_tag="PW109_001585"
     CDS             13622..14797
                     /gene="nusA"
                     /locus_tag="PW109_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2511414.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            14819..15112
                     /locus_tag="PW109_001586"
     CDS             14819..15112
                     /locus_tag="PW109_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2511415.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            15109..15420
                     /locus_tag="PW109_001587"
     CDS             15109..15420
                     /locus_tag="PW109_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2511416.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            15433..17739
                     /gene="infB"
                     /locus_tag="PW109_001588"
     CDS             15433..17739
                     /gene="infB"
                     /locus_tag="PW109_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2511417.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            17766..18113
                     /gene="rbfA"
                     /locus_tag="PW109_001589"
     CDS             17766..18113
                     /gene="rbfA"
                     /locus_tag="PW109_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2511418.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         6285 a         3180 c         4029 g         4647 t
ORIGIN      
        1 tgtttttctt gaattctttt tatctttcag gtaaactatt gaatgttgag cagaaaagta
       61 tatttgctct tgcgatacgg atcttttgtc tgtataatag ctgagggtgc tttatgcatc
      121 agcaaatcca cattcttatt ggattgtcgg taggtgcttg aaaaagtttg ccggcaaggt
      181 agaagataag aagaggaaaa ataaaaccaa atcggaggaa acaaacatgg cagtaatttc
      241 tatgaaacaa ttactagaag ccggcgtaca ctttggtcac caaactcgtc gctggaatcc
      301 aaaaatgaag aaatatatct tcacagaaag aaacggaatc tacatcattg acttgcaaaa
      361 aacagttaaa ttagtagatg cagcttatga ttacatgaag agcgttgctg aagaaggcgg
      421 cgtagcatta ttcgtaggta caaaaaaaca agcacaagaa gcaatcaaag aagaagcaac
      481 tcgtgcagga caatacttcg ttaaccatcg ttggttaggc ggaacattga caaactggga
      541 tactatccaa aaacgtatca gccgtttgaa acaaatcaat gcaatggaag aagatggaac
      601 ttttgaagtt ctacctaaaa aagaagttgc tggtttgaac aaagaacgtg aacgtcttga
      661 aaaattctta ggcggtatcg ctgatatgcc aagaatccca gatgtaatgt acatcgttga
      721 cccacgtaaa gaacgtattg ctgttcaaga agcacacaaa ttaaatatcc caatcgtagc
      781 tatggttgat acaaactgcg atccagatga aatcgacgta gtaatccctt caaacgacga
      841 tgcaattcgt gccgttaaat tgattacttc aaaaatggct gatgctttca tcgaaggaaa
      901 ccaaggtgaa gatcaagtag ttgaagaaga tttcacagaa gaaaacacag caacatcaat
      961 cgaagaaatc gtagatgttg ttgaaggcga caattcttca gcagaataat tttgaatgat
     1021 ggagctgttt caaaggctgg gcgacaagcc tcactctcct ttgaagcagc tttttttaaa
     1081 aagaaaaaca cacaggagga gttaaacatg gcagacgtta cagctaaaat ggtaaaagaa
     1141 ctacgcgaaa tgactggcgt aggaatgatg gatgcgaaaa aagcattagt tgaagtagaa
     1201 ggcgacatgg aaaaagcagt tgatctttta cgtgaaaaag gaatggcaaa ggctgcgaag
     1261 aaaaatgacc gcattgcagc tgaaggattg gcttcagttg ctgtgaaagg taacactgca
     1321 gcaatcgttg aagtaaactc agaaacagac tttgtttcta aaaacgaaat gttccaagat
     1381 ttagtaaaag aaattgcaga acttgttgct gaaaacaaac cagcagacat ggaagctgca
     1441 atgaaaatca aaacagataa aggtacaatt gaatctgact tgatcgaagc aactcaagtt
     1501 atcggagaaa aaatcagctt ccgtcgcttt gaagttgtag aaaaagacga caatgctgca
     1561 tttggtggat acttgcatat gggcggacgt attgccgtat tgactgttct agatggaaca
     1621 actgacgaaa cagttgcaag agacgtagct atgcacgtag cagctatcaa tcctcgttat
     1681 gtaaacgaat cacaaattcc agaagctgaa ttagaacatg aaaaaactgt tcttacagaa
     1741 caagcattga acgaaggcaa accagcgaat atcgttgaaa aaatggtaga aggacgcttg
     1801 aagaaattca aagcagaaat cgctttggtg gaccaaccat tcgttaaaga tcctgatatg
     1861 actgttgaaa aatacgttgc atcaaaaggc gcaactgtta aaacattcgt acgtttcgaa
     1921 gttggcgaag gtatcgaaaa acgcgaagat aactttgtag aagaagttat gagccaagtg
     1981 aaaaaataat tctgttgaat aacagacggg aacgcacggt ttgtgcgctc ccttttttta
     2041 agcaaaacaa gaaaaaccag tcttagagtg tcgaaatcat ctaagtatgg taaaatggca
     2101 atagaaaata ttggaggaat gaacatggtc acaccgaaat atcagcgtgt agtcttaaaa
     2161 ttgagtggag aagctttagc tggggatgca ggatttggaa tcaaaccacc agtaatcaaa
     2221 gaaatcatcc aagaaatcaa agaagttcac gagctgggcg ttgaaatggc aatcgttgtg
     2281 ggtggcggaa atatctggcg cggacaaatc ggagcacaaa tgggcatgga acgtgctcaa
     2341 gctgattaca tgggtatgtt agctacagtt atgaatgcct tagctttaca ggatacgttg
     2401 gaaaacgtgg gtgtaccgac acgcgtgcaa acttcgattg aaatgcgtca aatcgcagag
     2461 ccatatattc gccgtaaagc agaacgtcat ttagaaaaag gacggatcgt tatctttgca
     2521 ggcggtaccg gcaacccgta cttctcgaca gacacaacag cagcattacg agcggcagaa
     2581 atcggcgcag atgttatcct gatggctaaa aacaatgtag acggtgttta ttctgctgat
     2641 ccgaaagtag atgcaaatgc agtaaaattt gaagaactga ctcatctgga agttattgcc
     2701 aaaggattac aagtcatgga ttcaactgca agttcactaa gcatggataa tgatattcca
     2761 ctgcttgtct tcaatttaaa tgaacacgga aatattcgcc gtgcaatctt aggcgaaaat
     2821 atcggaacaa ctgtaagggg gaaataaaaa tggcagatgc aattatgaca gaagcaaaag
     2881 aaaaaatgaa aaaagcagca caaaatcttc aacgtgaact tgggcaaatc cgcgcaggcc
     2941 gtgcgaacgc tagccttttg gaccgtatta ctgtgaacta ctacggtgcg ccaacaccat
     3001 tgaaccaaat ggcatcaatc caaattccag aagcacgtgt tttaatgatc acaccttttg
     3061 acaagagcat tcttcaagat gttgaaaaag ctattatggc aagtgatatc ggaatcagtc
     3121 caaccaatga tggaaatgtc attcgcttag ttatcccgca actgacagaa gaacgcagaa
     3181 aagaattagc taaagatgtg aaaaaagaag cagaaaatgc aaaaatcgcg attcgtaaca
     3241 tccgtcgtga tgcgatcgat gaatataaaa aacaacaaaa aaatggtgat attaccgaag
     3301 atgatttgcg cggattagaa aaagacgtac aaacattgac agataacagc attaaagaag
     3361 tagataagat cgctgctgat aaagaaaaag aattattgga agtttaataa aatcataaaa
     3421 acagaggggc ggatcattcc gcttcctctg tttttattta tatttctttt atttaaaaat
     3481 taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac
     3541 agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag
     3601 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
     3661 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
     3721 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
     3781 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
     3841 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
     3901 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
     3961 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
     4021 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat
     4081 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
     4141 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
     4201 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
     4261 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
     4321 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
     4381 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg
     4441 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
     4501 gcaatcttag cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta
     4561 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     4621 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     4681 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     4741 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     4801 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     4861 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     4921 acgattgaag gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta
     4981 ttgctatacc cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg
     5041 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     5101 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     5161 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     5221 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     5281 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     5341 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     5401 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     5461 aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     5521 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     5581 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     5641 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     5701 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     5761 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     5821 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     5881 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt
     5941 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     6001 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     6061 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     6121 aggtaaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     6181 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     6241 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     6301 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     6361 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     6421 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     6481 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     6541 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     6601 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     6661 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     6721 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     6781 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     6841 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     6901 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     6961 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     7021 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     7081 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     7141 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg
     7201 caacggaaga aagtttggac gagacatata aagattacga aaaagcctat accgaagtat
     7261 tcaagcgttg tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga
     7321 aagactcaaa ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     7381 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     7441 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     7501 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     7561 cggatgaaca acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac
     7621 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     7681 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     7741 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     7801 tccactataa aaatgtgaat ccaaaacgcg atttcactgt gctttcttat gaagatcttc
     7861 gttttgtaca agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag
     7921 gaatcgaaat cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     7981 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     8041 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc
     8101 caaaaggaat tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc
     8161 aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     8221 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     8281 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     8341 aaactggcga aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct
     8401 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     8461 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     8521 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     8581 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     8641 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     8701 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     8761 gaatttacgc tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     8821 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     8881 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     8941 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     9001 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     9061 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     9121 gtggatgagc agcaagcgga acgtgtattg aaactttttg aagaacgcaa acaagaacag
     9181 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     9241 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc agctaggacg aaatattcca
     9301 gccgatgaac caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt
     9361 gaggggtacg tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact
     9421 ttgaaaatca cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa
     9481 gatgaacaag tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt
     9541 caagaagata cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa
     9601 catacacctc gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat
     9661 agcaatatga gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcagga
     9721 aaatggggac accgagctat cgccattaca gaccatggtg gtgcgcaagc ctttccggaa
     9781 gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc
     9841 gtagatgatg gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca
     9901 tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag
     9961 ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat
    10021 ccaggacatc cattgtcacg aacaacggta gatctgactg gtatcacgga tgaaatggtt
    10081 cgagggtcaa agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca
    10141 attttggtag cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg
    10201 tatggtattc ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg
    10261 tatcctcaat tcaaacgttt cggattaggt gtgttatcta aaaagtttgg tgttagcttg
    10321 gaacagcatc accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt
    10381 gtgaaagagg caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc
    10441 ggagaaggag attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat
    10501 caggcaggac tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa
    10561 cgcgtcccac gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc
    10621 tcagcttgtg ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca
    10681 agaaaccgag caaaattcta tgattatatt gaagttatgc caaaagcggt ctatgcgcct
    10741 ttgatcgagc aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg
    10801 gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg
    10861 aacgaagaag atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg
    10921 ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca
    10981 tttcactttt taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc
    11041 gcagatatct gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca
    11101 ggttcagaag atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat
    11161 ccgttacctg agatcattca aaaacgatta gaaaaagagt tgaattctat taatggtaat
    11221 ggcttctcgg ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc
    11281 tatctagtag gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc
    11341 actgaagtta atccattagc tcctcattat tattgtccag agtgccaata ttccgagttt
    11401 tttgaagatg gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt
    11461 ggtgcacgct taaacaaaga cggtcatgat attccatttg agacattttt aggattccat
    11521 ggagacaaag tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat
    11581 aactatacga aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca
    11641 gtcgccgaca aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag
    11701 tttcgaagtg cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc
    11761 ggccagcatc caggggggat catcgttatt cccgattata tggatgtcta tgattttacg
    11821 ccgatccaat atccagcaga cgatcaaaat tcagaatgga aaacgactca ttttgatttc
    11881 cactcgattc acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt
    11941 attcggatgc ttcaagattt atcagggatc gacccacaaa cgatccctac ggatgacccg
    12001 gaagtgatgc ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat
    12061 tccaaaacag ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgtta
    12121 gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt
    12181 acagacgtat ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg
    12241 gaagtaatcg gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc
    12301 gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat
    12361 gaattgcgag aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc
    12421 gattcttgtt caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg
    12481 atggctcttc gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac
    12541 ttttctgtac gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg
    12601 aaacaagcaa tgaaagaaat cacggacaag ggattagatg cttccgtaaa agaaaagaat
    12661 caactgacag tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg
    12721 attgatttgt ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca
    12781 ccatttagag ctgtcccaag tttgggaacc aacgtggcga aacaaatcgt tgaagcaaga
    12841 aaagacggtc catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca
    12901 ttgatcgaat atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta
    12961 tcgttattcg atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt
    13021 atggtatagt tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc
    13081 tttttaatgg aattacgcac ttaattttta aggaggcgaa acttttgagt agcgtcgttg
    13141 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
    13201 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
    13261 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
    13321 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
    13381 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
    13441 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
    13501 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
    13561 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
    13621 aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa
    13681 agaaatcgtg atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg
    13741 ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc
    13801 ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc
    13861 acttttaatc aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa
    13921 agattttgga cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga
    13981 agcagaaaga acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg
    14041 aatcgtcgaa cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt
    14101 tctttctaaa caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt
    14161 ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca
    14221 tccagaccta ttgcgtcgct tatttgaaca agaagtacca gaagtctatg atggaatcgt
    14281 tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa
    14341 tgatccaaat atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc
    14401 aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc
    14461 tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac
    14521 aaatccaaag gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa
    14581 acgcggacaa aatgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc
    14641 agaatctgac atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact
    14701 gcatgatgaa gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt
    14761 caacaatgaa acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat
    14821 gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa
    14881 agagatggtc cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa
    14941 aatgcctgga cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga
    15001 taaaaaaatt ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact
    15061 tcttgactat gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt
    15121 aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg
    15181 ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca
    15241 accgatgcaa gtgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt
    15301 ccttgctttg agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag
    15361 gtattcggaa taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag
    15421 gaaggtgatt gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct
    15481 agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt
    15541 gcgattacta gcagtgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa
    15601 caacaagctg cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt
    15661 aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc
    15721 ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg
    15781 ggaaatacca gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact
    15841 aacaataaca acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat
    15901 cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca
    15961 ccacgcaaat tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca
    16021 gatattgcga aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt
    16081 ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg
    16141 gactatggca tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc
    16201 ttcgaggcag aagaagtcga tccagataaa ttagtctcac gtccgccagt agtaacgatt
    16261 atgggacacg tagaccacgg gaaaacaacc ttattggata cattgcgcca ttcacgtgta
    16321 acaagcggcg aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat
    16381 ggtaaaccaa tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca
    16441 cgtggagcaa gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg
    16501 ccacaaacag ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct
    16561 gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg ttatgcaaga attaagtgag
    16621 tatgaattaa ttcctgaagc atggggtgga gaaacgattt ttgttgagat ctcagcgaaa
    16681 ttcggccaaa acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat
    16741 ttaaaagcag atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa
    16801 ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa cacttcacgt tggtgaccca
    16861 atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc
    16921 gataaagaag caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa
    16981 gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa
    17041 cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa gccgtgtgac tttggacaac
    17101 ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat
    17161 gtacaaggtt cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc
    17221 cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca
    17281 gcagccagca acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg
    17341 caagctgata ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa
    17401 gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt
    17461 caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat
    17521 gtaacagaag gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta
    17581 atctatgaag gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa
    17641 atgggttatg aatgtggtgc gatgattgaa aaattcaatg acatcaaagt agatgacgta
    17701 attgaaggat ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa
    17761 tcattatggc taactaccgt gatcgccgag tcggtcaaga aatcttaaaa gaagtcaatg
    17821 atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga
    17881 aagttacggg ggacttacag caagcaacga tctattacag cattttatct gacttagcat
    17941 cagataagaa aaaagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt
    18001 tagggcacaa tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag
    18061 ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa
    18121 atcccgcaaa tcctgtgaaa t
//
LOCUS       DANKHK010000050        17698 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000050 DANKHK010000000
VERSION     DANKHK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(60..422)
                     /locus_tag="PW109_001590"
     CDS             complement(60..422)
                     /locus_tag="PW109_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2511419.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            645..1397
                     /locus_tag="PW109_001591"
     CDS             645..1397
                     /locus_tag="PW109_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2511420.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1537..2460)
                     /locus_tag="PW109_001592"
     CDS             complement(1537..2460)
                     /locus_tag="PW109_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2511421.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2475..2747)
                     /locus_tag="PW109_001593"
     CDS             complement(2475..2747)
                     /locus_tag="PW109_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511422.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(3091..4278)
                     /locus_tag="PW109_001594"
     CDS             complement(3091..4278)
                     /locus_tag="PW109_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2511423.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(4691..6316)
                     /gene="groL"
                     /locus_tag="PW109_001595"
     CDS             complement(4691..6316)
                     /gene="groL"
                     /locus_tag="PW109_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2511424.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(6367..6651)
                     /gene="groES"
                     /locus_tag="PW109_001596"
     CDS             complement(6367..6651)
                     /gene="groES"
                     /locus_tag="PW109_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2511425.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            6876..7538
                     /locus_tag="PW109_001597"
     CDS             6876..7538
                     /locus_tag="PW109_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2511426.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(7614..8906)
                     /locus_tag="PW109_001598"
     CDS             complement(7614..8906)
                     /locus_tag="PW109_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2511427.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            9076..9705
                     /locus_tag="PW109_001599"
     CDS             9076..9705
                     /locus_tag="PW109_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2511428.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(9808..10617)
                     /locus_tag="PW109_001600"
     CDS             complement(9808..10617)
                     /locus_tag="PW109_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2511429.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10672..11541)
                     /locus_tag="PW109_001601"
     CDS             complement(10672..11541)
                     /locus_tag="PW109_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2511430.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(11542..12858)
                     /locus_tag="PW109_001602"
     CDS             complement(11542..12858)
                     /locus_tag="PW109_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511431.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(12855..14690)
                     /gene="walK"
                     /locus_tag="PW109_001603"
     CDS             complement(12855..14690)
                     /gene="walK"
                     /locus_tag="PW109_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2511432.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(14695..15399)
                     /locus_tag="PW109_001604"
     CDS             complement(14695..15399)
                     /locus_tag="PW109_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511433.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(15589..16449)
                     /locus_tag="PW109_001605"
     CDS             complement(15589..16449)
                     /locus_tag="PW109_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2511434.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(16436..17005)
                     /locus_tag="PW109_001606"
     CDS             complement(16436..17005)
                     /locus_tag="PW109_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2511435.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            17386..17458
                     /locus_tag="PW109_001607"
     tRNA            17386..17458
                     /locus_tag="PW109_001607"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17419..17421,aa:Lys,seq:ctt)
BASE COUNT         5112 a         3738 c         3024 g         5824 t
ORIGIN      
        1 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
       61 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
      121 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
      181 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
      241 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
      301 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
      361 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
      421 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
      481 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
      541 atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct
      601 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
      661 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
      721 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
      781 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
      841 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
      901 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
      961 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
     1021 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
     1081 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
     1141 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
     1201 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc
     1261 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
     1321 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
     1381 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
     1441 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
     1501 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
     1561 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
     1621 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
     1681 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
     1741 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
     1801 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
     1861 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
     1921 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
     1981 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
     2041 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
     2101 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
     2161 atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
     2221 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
     2281 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
     2341 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
     2401 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
     2461 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
     2521 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
     2581 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
     2641 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
     2701 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
     2761 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
     2821 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
     2881 ataatgatac tgtaaatata tctttattat tagtgaatgg agtcaagtcc acaatcttgt
     2941 gtaaaagttc aacctcatca aaagcacaat gttttgcgtt gtgaacctaa ctgccatgcc
     3001 tttctttctg aggagagagg tcaggagcag tagcgcagcg actcttgatg tctctcctca
     3061 gaaagaagag actcgcacag gttcatggtg ctaaatgtta ttcgaattaa attctaaata
     3121 ttttcgtgga gaagccagcc actcagtgtc tttttccatc aaaagtgttc caattatccg
     3181 agtgatggaa ttgatatttg gaaaaatccg aatcactcgt tctctccggc gaatctcttc
     3241 gttcaaccgt tccaacacat tggttgtacg aatccttcgg cggatatttt caggaaaaga
     3301 caaaatttga atagcatctt caaagccgtt ttctaaaatg tcgcaagcag cacttaattt
     3361 tgaatcacaa ccatattttt ccaagaagtg ctcttttctt tcacgtgtca attcaagttc
     3421 agaggcttta aagatactct tcaactcgtc ttttacatca gaagaaactt tctttggcag
     3481 cttatcaaaa atatttctca aaaagtgggc ttggcatctt tgccagctcg cacctaaaaa
     3541 gttcttgcga acagccttaa ctaacccttt gtgtgcatca gaaatgacca gttttacacc
     3601 gaacaaacct cgttgaacga acgattgaaa aacggtatcc caattatctt ccgattcgcc
     3661 gtcttgaaca tcaaaaccaa gaatcgtacg tcgtccatca gaatcaatgc cgataccaat
     3721 atatacacct tttgagacaa tacgatgatt ttcccttact ttcatataca gggcatcaca
     3781 catgaaaaat ggatattccg agccttctag gctccggttt ctccactcaa aaacaaatgg
     3841 atccaactgt ttcatcagat ttgaaacgaa tgatttagaa acagtcgcac catccgttag
     3901 cagttcaact gttttagtta ctttgcgagt ggagacacca gaaatgacca tttcaatcat
     3961 ggcagcgatc aaagcttttt cgcttcgctg atatctttca aagatagtag gactaaactt
     4021 accgtctctg gttcgtggaa catgaagttc taacgtacca attcgagttg taaacgaacg
     4081 atcgtagtaa ccatttcttt gtgattgccg ttcttctgaa cgttcatagg catttgcctg
     4141 aatatattga tttcgttgtt cttccatcag ctgattaaac atgatagtca taagtgtttt
     4201 tgcggtatca ttcgcaccgc tttcgtttat aattgcttga atttcttcgc tttcaaaagt
     4261 aaaatgtact tgagtcattg atatcagtcc tattcgagta tgttttctgc aaaaaacatt
     4321 gtactcctaa ctgagatcaa tggctttttt gatgcttcac ttttacacaa ttatatggac
     4381 gtaatcgtga atggatattt acttttaata gataaaatat tataactatt aaaaataact
     4441 attttcatta taattacaca caaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
     4501 tcacgctcta aacacgtata aacagcggaa gcagaagcaa ctccttcgga aataagctga
     4561 aattcacaaa aatttgaaaa gcaatttccg tgaatttctt cttatttctt ggagttaaac
     4621 gcttctgttc cagcctcttt ttatctgtct ttcactaatg atcagttgta gataagcaag
     4681 ttgctttttc ttacatcata ccgcccatca ttgatggatc cattgcagga gctgctggcg
     4741 ctgctggttc tggtttatct gcaacgactg cttctgtcgt taataacaag gcagaaacgg
     4801 aagcggcatt ttgcaaggca gaacgtgtta ctttagttgg gtctacgata cctgcttcaa
     4861 ccatgtttac ccattctcca gtagcagcat tgaaaccagt gcctagttcc acgttcttca
     4921 atttatcaac aatcactgat ccttcataac cagcgttttc agcgatttga cgaattggtt
     4981 cttctaaagc acgaacaacg atcttgatac ctgttgccac gtctccttct gcttctacag
     5041 cagaaacttt gctgataacg ttcactagag ctgtaccacc acctgagacc attccttctt
     5101 ctactgcggc acgtgtagca ttcaatgcat cttcaatgcg taattttaat tctttcaatt
     5161 ctgtttcagt agctgcacca actttaacaa cagctacacc acctgctaat ttagctaagc
     5221 gttcttgtaa tttttcgcgg tcaaaatcag aagttgtttc agcaatttgg tttttgatca
     5281 attgcacacg agcttcgatt gcttcttttt ctccagaacc ttctacgatc gttgtattgt
     5341 ctttatctac aacaacttta gaagcatttc ctaagttttc gattgttaca tctttcaatt
     5401 ctaaacctag atcgtcagta attactgtac cacctgtcaa aatagcgata tcttcaagca
     5461 ttgctttacg acgatcgccg aaaccaggag cttttacagc tactacgttg aatgttccac
     5521 ggattttgtt caatacaaga gttggcaatg cttcaccatc tacatcgtca gcaataatca
     5581 acaatggacg tgattgttgc aagatttgtt ctaacaatgg cagaatatct tggatgttag
     5641 agattttttt gtctgtgatc aagatatatg ggttttctag aacagcttcc attttgtcgt
     5701 tatctgttac catatattgt gataagtaac cacgatcaaa ctgcatacct tctacaacat
     5761 ctagttctgt ttcgatccct tttgattctt cgattgtgat gacaccgtcg tttccaactt
     5821 tttccattgc atcagcaatc aaatgtccaa ctttatcaga acccgaagaa acagctgcta
     5881 cttgtgcaat ggcttctttt gaatcaacaa cagttgagat attatgcaat tcttcgactg
     5941 ctgcttttgt tgccaattcg atcccgcgac gaatgcctag aggattagca cctgcagtta
     6001 cgttttttaa accttcgcga acgattgctt gtgtcagaac agttgctgtc gttgtaccgt
     6061 ctcctgcaat atcatttgtt tttgatgcta cttctgatac aagttttgca cccatgtttt
     6121 caaaatgatc ttctaattcg atttcttttg cgatcgtcac accatcattt gtgatcaatg
     6181 gagagccgta agatttttct agtacgacat ttcggccttt aggtcctaat gttactttca
     6241 ctgtatctgc taatttatct accccgcgta acatagctgc gcgtgcgtct tctgcaaatt
     6301 tcaattcttt tgccatgatt gattcacctc attgattatt tttgattatt tttttatctt
     6361 ccaaatttac tcaacaatcg ccatgatatc ttttcctgcg acgatcagat attcttttcc
     6421 ttcgtatttt acttctgtac cggcgtattt ttcgaacata acttggtcgc ctgcttttac
     6481 agcggcagga actttttcac cgttttctaa caaacggcct tcaccgactg caacaactgt
     6541 cccagtttgg ggtttctctt tagcagcaga agctaaaacg attcctccga cagttttttc
     6601 ttcttctttt gcgacttcga taatgacgcg atcacctaat ggttttaaca cgataaatcc
     6661 ctccgataac tcttttttta atctgttagc actcttaacc ttcgagtgct aacctacatt
     6721 tttaataata ctcattctcc ctcttttttg caagctttta aatcaaagaa aagaaatttt
     6781 attctgacag ctgcttattg aaattatgat ataattgaaa tcaatcgata taagcttgcc
     6841 ttgcatgctt gttatccctt aaaaggagga attacatgtc tttaaaaaaa tatagcttcc
     6901 tcagcatttt ttgctatggt ctaattttta tctcaccctt gatcctagct tcgataggac
     6961 ttgtaaaatc tacgtctgaa ttgatcacag caactgcagt catgtatata ctaggcgccg
     7021 ttgtcctagc tattttttat tttaagcaac gtgaaccctt agcgatcgaa tcagcggtaa
     7081 aaaaagcttc tgctccaaag attgttattt atggattggt tggaatcttt gtcgcattga
     7141 ttctgcaaag catcgctgtc atgttagaat ctgtattgtt tggcaaagca accccttccg
     7201 agaatacaca aaatatcatc cagatgatca tggaagcacc cgcttttatt ttggcgacaa
     7261 ctgttgctgg acccatcatg gaagagttcg tattccgccg cagtatactg gggatcatca
     7321 gtcgatattc aaatttttgg gtcggagcag tgatcagctc tttgctgttt gcgtttgctc
     7381 ataatgatgg tcatctgcta atctactttt tcctaggttt cttcttcagc ctagagtaca
     7441 aagcaactgg tcggatctgg acatcgatga tcacacacgt aggaatgaac acattagttg
     7501 ttcttgttca attagctgtc caaaaagggc tagtataaac atgactataa atactcctgt
     7561 catcctagat tggtgatttt cccaatctaa agcgacagga gtatttttgt tttttagtcg
     7621 ttcatctttt ctgctgctcc atcatttttt gtctgtcttt cctttttgca gtttgcagaa
     7681 tctatttttt tcaaaaatgg gaaataaatg aaaaaactaa ttgacaacac gataccttgt
     7741 aaaatcgctg caggcactcc gcctactaag aaacccgaga ggatcggcgg tgttgtccaa
     7801 gggacttgga ctgctgtaaa taaaggaacg aatccaaaat ataaagcgga ataggtaatc
     7861 aatcctgaaa cgaccggtgc gaaaataaaa ggaacagcca tcatcgggtt catcacgatc
     7921 ggtgtggaaa aaataattga ttcattgatg ttgaaaaagg ctggtaaaag agcaagattt
     7981 cctaattctc tgtatttctt cgatttcgca aaaaccgtca taaaaaatac agcccctaac
     8041 gtcaatccag cacccgtaac cgtcatgaac tgatcgagaa attgctgagt aacgatatgt
     8101 ccgccatttg ccaccgtcaa ctcttgtcct gaggctaaga tttctgcatt cgctaatgaa
     8161 ttcgcttgca agacgggact aaccaaccca ttgaccatcg cagatccatg aagaccaaat
     8221 aaccataaaa acggaacaaa aaaacctaat aaaagcacac caaacaacgt gtctatgaac
     8281 gattgaatgg gaagctggac atatgtccat actccttgaa caacagtcat tctgaacaac
     8341 tgttgaaaaa ccgctgaacc gataagcgta aaagtgacta taacaatgat tggaatcaat
     8401 gaagtaaaag actgccaaac attttcaggt atcttctctc tttgcttcca ccctttcgca
     8461 agtcttacta aacgtgcata gccccatccg atgatgagcc cagttgtaac tgctcctagt
     8521 atcccttcag cacctgtcca gacagtatct atctgagtat ttagtggctg aataagcagt
     8581 agataagcag acaaggctgt tatgccgact ggtaagcgat cgatgtcttc ttcttttgca
     8641 taagcgtatg caatggcaaa aacgatccaa aaggctatca catcaaatgt agcctggtat
     8701 acttgtgtaa aatacataga aaatcctgtt ttgtctatcc actgagcgat gtaatccaca
     8761 ggaaaatgag caaataagaa aaacagcgag ccaacaataa ttattgggat cgtgtaagtc
     8821 attccattac gcaaagcagt taagggtcta agattcacaa cagatgttat cattggtaaa
     8881 aaggcacgat ctaaccactc tttcatttct tatctccttc aacttctatt tttatcaatc
     8941 agttttctta atttaactat acccaatgat cacgaaccat gaaaggaatg cgcactgagc
     9001 ttttcaaaaa tcggattctt tgacgaatat tcagcaactg cttacaatgg acaggaataa
     9061 aaaaggaggt tttttatgaa aattgcactc attggagcta ctggccatgc aggttcattt
     9121 attttagacg aagcattgtc cagagattta gacgtgacgg caatcgtgcg tcatccagaa
     9181 aaattgccat cagatgttcc gtttatacaa aaggatttat ttgagttgac aactactgat
     9241 cttgcatctt ttgatgttgt cgttgatgct ttcaacgctc ccaaaggaaa agaagaaatg
     9301 catcaaactt ctttgtccca tttgattgag atgcttgaag gaaccgatac acgcttaatt
     9361 gtcgttgggg gtgcctcttc gttattctta gatacagaaa agacgactcg tatgattgac
     9421 caagtaccta aaaatgcacc cttctatcca acagctttca atatgtacga aggattgtta
     9481 aaattgaaac aatctaaaaa tctacattgg acttatatca gtccagcttc tttatttgat
     9541 ccacatggca aacgtacagg tacttatttg ttaagtgatg attatttaca aagaaacgct
     9601 gctggcgaca gtacgatcag catggcagat tacgcgattg cattagtaga tgagatactg
     9661 gatgagaaac atgaaaagca gcacatcagt gtagtcagtc gataatacgg ttattttttt
     9721 aatatgctaa aaaaataaaa atagaagata aaaccaaccc tgattgattt tatcttctgc
     9781 tttttatatt tttattgatt gaactgttta aatcgaaaat agctcagttg cttcatctgg
     9841 atctgtatca tagatttcaa aatcatgtcc aacgccaaaa tctttttctt tcaaaacatt
     9901 ttccattgtt aaatgagcaa gttctttcat attgttttcc ttgctaagat ggccaagata
     9961 gattcgtttg gtccgattac cgatcacatc agtcatgaca agtgcaccgt cttcgttgga
    10021 aagatgtcct ctatctccta aaatacgctg ttttaaattc cacggataag gtcccatgcg
    10081 taacatctca agatcatgat tactttccat caaataacca tcggcatttt caatgacacc
    10141 tcgcatataa tcactgcaat atccggtgtc cgtcaacatg acgaatgacc gattttcttt
    10201 atggaaacga taaaattgcg gagccgctgc gtcatgggag acgccgaaac tctcaatatc
    10261 gatatcgccg aatgttctga ctttccccat ctcaaataga tgtttttgtt ccactggtac
    10321 atttccaatc agtggttcca tcgcttccca agtctgctga ttggcaaaaa catccaaatg
    10381 atacttacga gccaagacac ccacgccatg tatatgatct cgatgttcat gcgtcacaag
    10441 tattccatct aattggtccg gtgtccgatc aatttgagcc atcaaggaag tgattttttt
    10501 accgctaagt cctgcatcga ccaaaagccg ttttttttca ctttcgatat aaagtgaatt
    10561 acccgaactt ccacttgcta ggatactgat tttaaaagct ggtttttctt cagccatttg
    10621 actcctactt cccttcttac tttgtaaact tttttcatta tactcgcttt tttattgatc
    10681 ttccactttt ggtacagtat tgtttgtcac gatcgtattg ctgaaagcgt tgacactctc
    10741 aatttggacc actttatcgc ttgtttcgat cgctacatgc caaacaggaa catacacatt
    10801 tttttcccgg actttcagaa tacaggaata agctagctga cgccataaaa tttttgatcc
    10861 tcttggtatt ttgttattga tgtacaatgt attgattgct tcctcttccg tatacagttc
    10921 cgtcttttcg cgcagctgct cgatatcaga taaatgcgtc tgcatgtatt tcgtgatgct
    10981 ataatcatta tcgttttttt ctaaagaaag aacgattttt gctgtatcat cattgaatgg
    11041 gatgccttca tactcttggc ttgcttggat ttctagtggt tcttcttcta aatgagaaaa
    11101 gtttttcgtg tacacataat cattaccgaa taaaatattg tctttgttag ataaaaaatc
    11161 ttccaaggaa ctactaattt tatctggatc gatcacgttc ttttcagcat ctgaactgga
    11221 taaagatttc gtcaaaacat caccgtctaa aaacacacca cttgccagca agccgctttt
    11281 accagccttg cgataattcg ctaatgaagt gctaagattg gtctgctccg cgctaagata
    11341 atagccttgc ttgttctcag acgaaatctt ccccgtgtaa gtgatatcgt cgctagccag
    11401 acgttgttgg attgattcag tctgattaga gcgaatgaca ttgttttctt ggtataccgc
    11461 gtcatgataa ctgctgaata aaaacacatt caaccctaaa aaagcgacga aaaatatcca
    11521 ttcgattttt ttgaaatcca attaattcgc ctccaatcca ggtaattttt ccagaaggtc
    11581 gttagctgaa taccagttat ctttatattt cacataccat tcaggtaaga gatcaacgac
    11641 acgattcgtt tctttgacat tttgccatgt atagccaacg atcactgaac caatcttgtt
    11701 gctatctgct cccttagctt ttaactgctg gatgatttca ttcgtacttg caagttctac
    11761 ttcttcgtct gaaggtatcg gcacttgaat cgtattcaaa ctggtttgga tattcacttg
    11821 cacagaacca ttcgtttcac cgccaacttc cacaccaatc tgtcctctgc tgtctgtccc
    11881 aaaaaccggg aagccttcca cgaatatccg ataatcgatc gtattgtcac tgcggtcaaa
    11941 atatctgagg ttccctaaag aactgcctaa tttgctaata taggggaaac tttcagaaaa
    12001 tacactagca ttttcttttt tctctggcag ctctccacga aaatcaacta cttgcgtttc
    12061 cgaatggata gacattgttt cattcccacc gtaaaaaatc aattcattgc tttcatcatt
    12121 atttttcacg ctatctggtt tctggaaaaa agcatttcta aaaagtgtat agggttgctg
    12181 tgacaggata taactatact ttttcagttt gatcgaatgt ttcgtagcat attgcgtgtc
    12241 gatcgtcgat ggttctgttg tcatttctgt ccattttgtg tcttttgctg ttaattcttt
    12301 attgatatct gtccaattca acgaaatatt tgcttcatat acgagatggt tcgaataatt
    12361 caaaaatcgg atttttttat ttccttgatc gaattgtatt ctagtaaaat acatattttc
    12421 gctttctttt gccaagttcg acacatctaa atctaaatca aatgcttcct tgtattctct
    12481 tagcaaaaaa ggcgcattat aggccaactc aaaaccagag ttcagatttt tcacctgttc
    12541 aaactgtgat ttgtctcctg aaactacttc agaaagcttt ccaaatcttg ctttatcgat
    12601 gatcaattgc aatcggtaca cgagattttc actgttggtt tcttggatca ttcccgaatg
    12661 gtgccatgta acatgaagcg gaagaaagac ttcggaagtt tttcgattgc tttgagactc
    12721 gatcatttga gtgtttgtct catctacgtt gattgaactt tttcctgctg ggctcagcca
    12781 gattgcataa gagaagtata tgcttaacgc aaccatcaaa atgagtccta tacgaacgac
    12841 tttttcagag attctcattc ccaccattcc tcctcataag gttcatatgg cagcgagata
    12901 taaaaagtcg acccttttcc ttccttgctc tctgcccaaa tagcgcctcg atgcgcctta
    12961 atcacttctc tagaaatagc taatccaagt cctgtcccac cttgtgctct tgcacgtgct
    13021 ttatctactc gatagaaccg ctcgaacact cggttcagat cttttttagg aatccccaat
    13081 ccttgatcgg tgatacttaa aaccacgtta ttatgcgttt ctaataagcg acaagtaatc
    13141 gttcccccat ctggtgaata cttgatcgcg ttattcatga tattatcgac tacttggatg
    13201 attttatctg tatctacttc gacccaaaga tctctttgcg taaattcacg acggatctta
    13261 taattttttt cctgatttcc caccatcata tcaaaccggt caagaacaaa attcactaac
    13321 tcgttgaaat tgacatattc taattgaagc tgggtattac ctgtatccat gcgagataga
    13381 tttaagaggt cattgatcat tcgaatcatt cggtctgttt catctagcgt taccttcaaa
    13441 aaatttggtg ctacttcctc gtctttccac gcaccttcgc tcaatgcttc gatataacta
    13501 cgcatacttg tcaacggtgt acgaagttca tgggaaacat tagagacgaa ttcacgacgt
    13561 tcccgttctg ttttttcttg ttctgtcaca tcatgcaaga cagctactaa tccgctgata
    13621 aaacctgatt cgcgacggat catagaaaag tctactcgca agatcatctg atcactttcc
    13681 aatgtactgg ttgaacggtc aagtaccatt tcgtttggat cttctaataa tttccgcaac
    13741 gtatattctt cttcgatatc taaaagtgtc aaaatcgatt gtccgattgc ttcctcgttt
    13801 ttgatatcta gcaaggaaac agccatatca ttgatcgtaa tgactttacc cctgcgatct
    13861 gtcgcaatga cgccgtctgt catatgggat agcacgctat ccaaacgatt gcgttcagct
    13921 tccattgtat cttgagcttc ttcaatacgt tcagctaact gattgaaggt atcagccagc
    13981 tgtccaagtt catcttgtcc atgcactttg actttccgac tgtagtctcc tctagcgatt
    14041 cgaatggctt gttctctcat ttcaccgatc ggctgtgtaa tcgaacgagc aactaaaaca
    14101 gatacgatca ttgaaataaa tgccgcaatt agtgaggcag taaagaaaat gactgctgtg
    14161 ttattgattt ccgaatattt ttgttcgata ttacttttga cgtaaagagc cccgataacg
    14221 gcatcgccgg tcggggacag gatcggttga acattgatat tcacccgttt atcgtcgtta
    14281 tctaaagcaa cataacgttt agtcgtgaaa tcattgatat caacataatc atttttcttt
    14341 cccacagcgc tttgttcatt gacatcggtt gttcctcgga tgatcccttt ttcgtcaacg
    14401 acccgtattt cgatgatatc gctagtcgca ctgttttcaa taaatctttt gatattcgca
    14461 tttacctctt catcatctgc attatctttt cgattcaatt ccgggctgat tgttgttgct
    14521 aatgattcca ccgtttgatt catatcttta ataaatgtat tgatcgtgga acgttctaaa
    14581 ccacgtataa agtaggcacc aataatttcg atcgagatca ataaaatcag aataaaggta
    14641 atagcgatct taaaattgac tgatcgaaaa aagcggactt ttcttttcat ccaattactc
    14701 ctgttctggg ttacgcaaat agtaaccgac accacgacgt gtcactaaat aatttggatg
    14761 gcttggatta tcttcgatct tttcacgcaa acgacgtaca gttacatcca ctgttcgtac
    14821 atctccaaaa taatcataac cccaaaccgt ttgtaataaa tgttctcttg tcatcacttg
    14881 tccaaggtgc ttcgccaagt aaaagagtag ttcgaattca cgatgtgtta actcgattgt
    14941 ttctcctcgt ttcgtcacca tgtaagcttc tggatggatc gtcaaatcac caatcgtcaa
    15001 ttccgcaggt gctgcctctt ctggttcctt cgcagccgtg gcaccgcgac gaagatttgc
    15061 tttgacacgt gcaactaatt ctcggttgga aaatggcttg gtcacgtaat catcggcgcc
    15121 taactctaag cctaggactt tatcaatttc agaatctttg gctgttacca taatgatcgg
    15181 catatcataa gttttgcgta cctcacgagc aacttctaag ccgtccattt taggtagcat
    15241 caaatcaagt aaaatcaaat caggttctac ctcagcaact ttttccaagg cttcttcccc
    15301 atcatatgca gtataaacat catagccttc tttggctaaa ttgaatttga cgatatccga
    15361 aatcggcttc tcatcatcga caactaaaac ttttttcata aagagccacc tcttatatat
    15421 tattgtcata ccttctcatt ataccgtatt tctgcttttg tttcatcttt gcctataaaa
    15481 caaaagagaa aattaaaatg aaagtgcaaa atactttatt attttttgtt ctaacttcaa
    15541 taaaaaagga aaacactgat tttcacctca gtgtttcctt tttttgattt atccttctaa
    15601 aacttgtaca ccgacaccca ataatcccgg accagtgtgg actactaatg ccggagaaat
    15661 ctggccaaag tagatttggt ttgcttgtgg gaactctctt ttaagtctcg cttccatttc
    15721 aattgcttct tctttcgcat ccccatgggc aacagctaag ttgaagacct ttgcattacc
    15781 gattttttct ttgacgtaag caaccgtttt atccaaactt tttttacgtc ccctagcttt
    15841 tcctactgtg taataaatcc cttcttcatt acatgagatg attgggttaa gctttaaagc
    15901 ggttcctaca atagaagcaa ctaaaccgat ccgtccacct ttttgaagat attccaatgt
    15961 cgcaacatta aagaaaactt ttgttctttc gactaactca ttcagattac tgatcagttg
    16021 atcccattct actccttctt ccagccattt tgctgcttgg atcgcagaaa aacctgcacc
    16081 aataccaata tttttcgtat ctagtacata tgcttccaat tcttcttgct gttgtgcaat
    16141 taaacgaacc acattatatg tcccgctcaa tccactggaa attgttaccg caagtacctt
    16201 ttcataaccg tcttttttga tttgctcaaa gatttctgtg atcgactcac catccggcaa
    16261 agatgtacca gggatttcct ctggcaaacg ttgatagatc tcttcagagg taatgtcaat
    16321 tttatctgta tatacctgat ctttataaat aattttcaat gggatcatat acatctcgta
    16381 tttttttatc aattcttctg gcacatccgt acctgaatct actaaaattg cgattttatt
    16441 gtttttcatt tgttttctcc tcttttaaat attcggtttc tagaacaatc gttttttccg
    16501 caagaagttt cattgcaaac gataaagttg ccgctcttac agcaatatat tctacagggg
    16561 ttgcctggtc gaatttttgc ggatgagatt taccttgtgc taattggctg accatccaca
    16621 cagctgcttc ttgttcatcg caaaatagat tataggcttc acgaattcct actgtttttc
    16681 cttggaacaa tatcccttct ttgatttcag gaatagtcaa tacttgtttc aacgtagtga
    16741 ttgcaagtaa aaatgctacg tgttctttgt tatatctttt tttaactggt gctggtatca
    16801 aacctagttt aacgtaattg tttaccatag aagaggtaag caatggatgc ttttctcctt
    16861 gtatcacagg tgaaagataa cgatcaacta acgttctgac ttggtccata taaagttcta
    16921 agtcagggag ttcttcccag tgaggcagac ggaatgtttc tagttccttc ccccactcca
    16981 ttaattgctg acgcattttt tccatcataa ccgctccttt tttcatttca taaacatgat
    17041 atcagactta tgataaacat tcaatctagt tgctttaact aggtgatttg attagcggga
    17101 ttccatgaaa acaaaaaaca agaaggctaa gccttcttgt cttttatcgt taagaacagg
    17161 agacccgccg gggagacggc gggaaaggag taaaaaatga aaaaagtgtt agggtttgtt
    17221 ctcttgcaag aactttgttt gttggtatgc ttatataata gcgagcaaat gtgaataatc
    17281 tatgaatgat ttcctataag gaagtaaaga tttttctgat tttctttaaa tgtagtcttg
    17341 cattttaaaa ttcttagtgc tactattatt aagtactttt tgtacgggcc gttagctcag
    17401 ttggtagagc agctgactct taatcagcgg gtcgcgggtt cgagcccctc acggcccatt
    17461 gggtgccaaa cccacggaac aggtaaatgt tggtgcgaaa gcatttcgaa aacatttacg
    17521 ctgttctttt tttattttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt
    17581 atgttacact caaattgtaa tacccgctaa agcatacaag gagagtgaac ataaatgact
    17641 tatacccatc ttacaccaaa cgagcttgta atgatagaag catattttca tcaagaaa
//
LOCUS       DANKHK010000051        17444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000051 DANKHK010000000
VERSION     DANKHK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..1539)
                     /locus_tag="PW109_001608"
     CDS             complement(<1..1539)
                     /locus_tag="PW109_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2511436.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEF"
     gene            complement(1543..1824)
                     /locus_tag="PW109_001609"
     CDS             complement(1543..1824)
                     /locus_tag="PW109_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2511437.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1821..2744)
                     /locus_tag="PW109_001610"
     CDS             complement(1821..2744)
                     /locus_tag="PW109_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2511438.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2741..3619)
                     /locus_tag="PW109_001611"
     CDS             complement(2741..3619)
                     /locus_tag="PW109_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2511439.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3606..4943)
                     /locus_tag="PW109_001612"
     CDS             complement(3606..4943)
                     /locus_tag="PW109_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2511440.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(4946..6049)
                     /locus_tag="PW109_001613"
     CDS             complement(4946..6049)
                     /locus_tag="PW109_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2511441.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6123..7631)
                     /locus_tag="PW109_001614"
     CDS             complement(6123..7631)
                     /locus_tag="PW109_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2511442.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7597..8289)
                     /locus_tag="PW109_001615"
     CDS             complement(7597..8289)
                     /locus_tag="PW109_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2511443.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8311..9285)
                     /locus_tag="PW109_001616"
     CDS             complement(8311..9285)
                     /locus_tag="PW109_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2511444.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9585..10457
                     /locus_tag="PW109_001617"
     CDS             9585..10457
                     /locus_tag="PW109_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2511445.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11039..13237)
                     /locus_tag="PW109_001618"
     CDS             complement(11039..13237)
                     /locus_tag="PW109_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511446.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13469..14461
                     /locus_tag="PW109_001619"
     CDS             13469..14461
                     /locus_tag="PW109_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2511447.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14521..15942
                     /locus_tag="PW109_001620"
     CDS             14521..15942
                     /locus_tag="PW109_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2511448.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15935..17434
                     /locus_tag="PW109_001621"
     CDS             15935..17434
                     /locus_tag="PW109_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2511449.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
BASE COUNT         5180 a         3594 c         3048 g         5622 t
ORIGIN      
        1 aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg ctatcccgat
       61 aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac ctactttctt
      121 ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct ttacagtgac
      181 tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc
      241 gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag taacataaca
      301 ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat taaaattatt
      361 aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa
      421 caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta aaaaacctgg
      481 tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat tccagttctt
      541 ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt gtacctcttc
      601 atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa gtcttaacca
      661 atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa tagccgcacc
      721 gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg tttgtactaa
      781 gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg tcagcatagg
      841 aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa ctgtaactga
      901 atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt tacgaaaata
      961 caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat agatgggacc
     1021 atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg atttattcgg
     1081 tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg tttcaagatc
     1141 atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg accaaggacc
     1201 tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac ggcctgcact
     1261 tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct gtggcgccca
     1321 tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa tatccgttgt
     1381 atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa gccattcaaa
     1441 agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat ctggattgaa
     1501 tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc cttttctcat
     1561 tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa taaacaacac
     1621 aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta aagacaaagt
     1681 tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag acatattact
     1741 taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga ccattcctag
     1801 caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg tatccattca
     1861 ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac agtctgttca
     1921 tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc tatcttacac
     1981 gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac gtttctagta
     2041 tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg atttttcggc
     2101 gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat atgacactga
     2161 tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat ccagcaatat
     2221 tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa aggagtacca
     2281 tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc agatagtcga
     2341 cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg tcctaccttt
     2401 tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt cgatccttct
     2461 aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata ctcctgtttt
     2521 gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa attggataat
     2581 ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt tggatggcta
     2641 tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc cgtcggggta
     2701 tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc tcccttatga
     2761 attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt aaagaaaaag
     2821 aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct tttctcagct
     2881 tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta agacgagggc
     2941 gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga tttggcacat
     3001 agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg atgtccacct
     3061 gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc caccagctta
     3121 ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg tcttcccttg
     3181 tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat aacgtttttc
     3241 ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt tcttcaaatc
     3301 cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct gcaaaagatg
     3361 gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa aaatctccgc
     3421 cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga taagcataga
     3481 gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc ataaaaccgt
     3541 tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat ggagcaccta
     3601 aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc catattttct
     3661 gattgatttg attggaattt ctcaatactg atttctttag tattttctaa tagaaatgct
     3721 ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc aaagccaata
     3781 taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac accgggaact
     3841 gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat gaattgttcc
     3901 gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat attcgaaaaa
     3961 tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt ttcccataca
     4021 tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa tggacacatc
     4081 acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc tcgacctcgt
     4141 ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga aatggtaata
     4201 ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc atggaccatc
     4261 gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc ttcagtccct
     4321 gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat acgcacattt
     4381 ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt caagatcgtc
     4441 aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact tggtgactgg
     4501 atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat caatttagga
     4561 cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt ttttccattt
     4621 ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc atagatacag
     4681 gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg ataatctttt
     4741 ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac attgcttttt
     4801 aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc gcctccgtca
     4861 cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc aattgatgcg
     4921 ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat gcttgcaaga
     4981 atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag taagcatatg
     5041 aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta ttcaagttca
     5101 gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt gatttcttca
     5161 cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta gccattgcgt
     5221 aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt gtttctgttg
     5281 ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca gccagtgtct
     5341 tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat tcataggtat
     5401 ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg gatagacctg
     5461 tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc agcaaatttg
     5521 tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca tcttggtaat
     5581 ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc atatataagc
     5641 catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct tcggatgtgc
     5701 gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat ttttcttcgc
     5761 ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg atattgtatt
     5821 cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg atattgtctt
     5881 ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg aaccctttaa
     5941 gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca tataagcgag
     6001 gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt atttgctctc
     6061 ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc caatcgtcta
     6121 tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg atcgcctcct
     6181 tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg tcccatgatg
     6241 tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc attgttcgtc
     6301 ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc tttgatcaat
     6361 tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac ttcactagga
     6421 cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat aaattcgata
     6481 gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat caatttatac
     6541 acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg tgcttcgtct
     6601 tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc tgaccaaaca
     6661 tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc catatcatct
     6721 tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt gtagattcca
     6781 gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc aaagtcttgt
     6841 gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat atactcttct
     6901 gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt tggtgctcca
     6961 aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc acgatcgcca
     7021 aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa ttgtttagct
     7081 acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc caaccagtct
     7141 tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc gtacaaggca
     7201 ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt tttatagtct
     7261 tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg gacagaagag
     7321 tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga agccaatgca
     7381 gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag ttccgtatcg
     7441 gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg atgcaaaata
     7501 tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt acttgaactg
     7561 ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt tacgagcgtt
     7621 gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt cttctgtttt
     7681 ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg tccacgtgac
     7741 tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg tttggatgat
     7801 catcagataa atccctacca tacttaatat caacactaat gagtaatgaa gatgtgagaa
     7861 tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat aatgagccat
     7921 gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat acaaagcgat
     7981 cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc cgatggcgcc
     8041 atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg tgaagtacgt
     8101 tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg caaccatcgc
     8161 atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac cgccccacag
     8221 aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt tatctaatga
     8281 ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc ctagcttttt
     8341 ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa acaatcccat
     8401 tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat aggtgtcgaa
     8461 aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct cgaaccctaa
     8521 gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac gaattgccgg
     8581 caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg cagcttcata
     8641 caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc agcccaactc
     8701 tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg ttagaaaact
     8761 gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt caacattcaa
     8821 aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga tgtaaatgat
     8881 cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta aagacaaaat
     8941 gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg tatttcttaa
     9001 taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac cgacccaagg
     9061 acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga gtccccccat
     9121 aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca catataaaaa
     9181 tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt cttctttttt
     9241 cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc cctccttagt
     9301 tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt acgtatataa
     9361 tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta tacaaaacga
     9421 taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt tttctatctg
     9481 acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa ttgaacatat
     9541 agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa aagaaagtct
     9601 ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg ctagctttga
     9661 tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca caagaagtcg
     9721 tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat cgctacattc
     9781 tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca ggtgtcggaa
     9841 cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga aagtatttgt
     9901 ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc ggggggatga
     9961 ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg gccatgatcc
    10021 tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc caagccttgc
    10081 cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt gtatttttca
    10141 aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt tatgctgtta
    10201 cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct aaatggccga
    10261 tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca gaaagtagtg
    10321 ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt attgcaacag
    10381 tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga gcactcgtag
    10441 gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca gaagtggtca
    10501 gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt gaaatttcgg
    10561 cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg aaagagacgt
    10621 tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc cacaggagca
    10681 ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc ttattgtcga
    10741 tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga cttttgtcac
    10801 agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa tactagggtt
    10861 attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct tgattaaacg
    10921 gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca tgaagtgctg
    10981 ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc ctcttgattc
    11041 acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta aagaaacgaa
    11101 taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg gagaggttag
    11161 tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa tcaccaaatt
    11221 tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa tgcaacctat
    11281 cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata tgcataatat
    11341 tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct gtgattggca
    11401 gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg ttcagtgctt
    11461 ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat aactgtccta
    11521 attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa ggatttttat
    11581 tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc tgatttccag
    11641 aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata agtgtttttt
    11701 catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca gtatttacac
    11761 gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc agcgtttcga
    11821 accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca cgtttttgaa
    11881 tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta tccattcgat
    11941 aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt tcctttggca
    12001 caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc ccaccgtgac
    12061 tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt tgagaactat
    12121 aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt ctaaataaac
    12181 taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca cttaacgctt
    12241 ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct ttcccactga
    12301 atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg gcaaatctgt
    12361 ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac actgcaacta
    12421 gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact tctaatctgt
    12481 aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt tctcttatca
    12541 ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc tctttatttt
    12601 ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt atgtcttctc
    12661 cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt cccaatgcat
    12721 atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg tttttctttt
    12781 gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca acttcctctt
    12841 cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa ctgactaaat
    12901 cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc ctgatttcag
    12961 aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag aaatttttgg
    13021 tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca acgttagttt
    13081 taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc gattcatagg
    13141 tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat aaaaacatcg
    13201 gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc tccttttaaa
    13261 ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga gcattccccg
    13321 ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt gtgcaatctt
    13381 ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat gcgttaacaa
    13441 aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg ctaaagcagc
    13501 tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa taaaaaatga
    13561 aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa actatagtgc
    13621 aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa gtttgcataa
    13681 aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag tattagacac
    13741 aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa tcaacctgca
    13801 acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt tcatcgacta
    13861 tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg atccagcggt
    13921 tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt acggtattgg
    13981 atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt cgacaaaaga
    14041 acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca atctagccta
    14101 ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat acctattatc
    14161 actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga ttggtgcatt
    14221 gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga tcgggttcga
    14281 tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta agcctttgaa
    14341 agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca ggacgatcga
    14401 acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca attacttgta
    14461 aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa ggaagtttat
    14521 atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa agttcttcag
    14581 tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca gctgaataac
    14641 caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg tcttggaaat
    14701 atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag caatggcgaa
    14761 atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc ttatgatgaa
    14821 tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt ctccaataca
    14881 acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc cccacagagc
    14941 ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa agctggcttt
    15001 accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga aatcatttta
    15061 cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga aaaagaaaat
    15121 gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga agatgcaggt
    15181 gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga accattcatg
    15241 ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa acaggctggc
    15301 ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt gcatttgctt
    15361 aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga aactgtagaa
    15421 gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa tctagaattg
    15481 attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca aatcaaagaa
    15541 cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa tagtgtttca
    15601 aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca aaatcaggta
    15661 ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg cgatcaagta
    15721 aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc tgataatgga
    15781 acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt gtctcaaata
    15841 ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa aggtgtccgg
    15901 tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc aaccaacaat
    15961 gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca
    16021 actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat
    16081 tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca
    16141 tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct
    16201 ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga
    16261 gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt
    16321 gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt
    16381 caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta
    16441 tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt
    16501 aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt
    16561 tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc
    16621 tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc
    16681 ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg
    16741 cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga
    16801 ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc
    16861 agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt
    16921 gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc
    16981 tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca
    17041 aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc
    17101 agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc
    17161 atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt
    17221 cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga
    17281 ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca
    17341 aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga
    17401 aattgccatt tttaaaaacg gagtaacact ttgattttca taaa
//
LOCUS       DANKHK010000052        17133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000052 DANKHK010000000
VERSION     DANKHK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            547..1797
                     /locus_tag="PW109_001622"
     CDS             547..1797
                     /locus_tag="PW109_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2511450.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            1816..2826
                     /locus_tag="PW109_001623"
     CDS             1816..2826
                     /locus_tag="PW109_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDL2511451.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            2836..3489
                     /locus_tag="PW109_001624"
     CDS             2836..3489
                     /locus_tag="PW109_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2511452.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3508..4506
                     /locus_tag="PW109_001625"
     CDS             3508..4506
                     /locus_tag="PW109_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2511453.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            4582..5343
                     /locus_tag="PW109_001626"
     CDS             4582..5343
                     /locus_tag="PW109_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDL2511454.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5361..6467
                     /locus_tag="PW109_001627"
     CDS             5361..6467
                     /locus_tag="PW109_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2511455.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6889..7344
                     /locus_tag="PW109_001628"
     CDS             6889..7344
                     /locus_tag="PW109_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HDL2511456.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7358..8131
                     /locus_tag="PW109_001629"
     CDS             7358..8131
                     /locus_tag="PW109_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2511457.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8118..9248
                     /locus_tag="PW109_001630"
     CDS             8118..9248
                     /locus_tag="PW109_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2511458.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9279..10514
                     /locus_tag="PW109_001631"
     CDS             9279..10514
                     /locus_tag="PW109_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511459.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10539..11648
                     /locus_tag="PW109_001632"
     CDS             10539..11648
                     /locus_tag="PW109_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511460.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            11690..13024
                     /gene="gmhB"
                     /locus_tag="PW109_001633"
     CDS             11690..13024
                     /gene="gmhB"
                     /locus_tag="PW109_001633"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL2511461.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13059..14696
                     /locus_tag="PW109_001634"
     CDS             13059..14696
                     /locus_tag="PW109_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDL2511462.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            14798..15193
                     /locus_tag="PW109_001635"
     CDS             14798..15193
                     /locus_tag="PW109_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDL2511463.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15211..15942
                     /locus_tag="PW109_001636"
     CDS             15211..15942
                     /locus_tag="PW109_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2511464.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            15966..>17133
                     /locus_tag="PW109_001637"
     CDS             15966..>17133
                     /locus_tag="PW109_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2511465.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYG"
BASE COUNT         5495 a         2428 c         3793 g         5417 t
ORIGIN      
        1 aattattatc gtaggatgtg atgatgaaag ccgtgaaatt gttcaagtat ttaccaatgt
       61 ctccctgcca gtaaatccgc ttagaatttg agccttgaaa atgtgcaatg ataaaaaaca
      121 aaaactcgct caaaatagac agtttggggt gggaatagac caataatgta ccaaacaaac
      181 ttccatttgg ggtgaaaata ggtcaataaa gcgatgttaa acataccttt ggagtaagaa
      241 tagagggtaa taagtgaatt tataaacttt ttggtgactg gttgagtgga aaatgtaggt
      301 acatttcaat gcgaaaatgt attataaccc caaacgaggt caataaaaat gactcaaact
      361 tgcaatataa ccctaaacgg gtaggggatt tatgaaaaat tgaatttgcc ggcgtaggag
      421 ctggctgttt taagtatgaa tgaacacagc tttcgggctg ttttatataa atagcaggtt
      481 actgttcaca aacagcattt tgttaagaaa gtagtgacca cagcagtggt ggaaagagga
      541 aatattatga agaaacagta taacattgca gtcgccggca ccggttatgt tggtttgagt
      601 attgccactc tgttggcaca gcaccataag gttacagcgg tggatatcat tccagagaag
      661 gttgaactga ttaataaccg taaatctcca atccaggatg attatattga aaagtacttg
      721 gctgagaaag aactgaatct gaccgcaaca ttggatgcaa aggcagcata ccagtctgcg
      781 gattttgttg ttattgcggc tcctactaac tacaatagcc gcactcaaca ttttgacaca
      841 tccgctgttg aggctgttat taggctggtc atggaagtaa atcctgatgc cattatggtt
      901 attaagtcca ccattcctgt tggatacacc gcttctgttc gtgtaaagac tggtagcaag
      961 aatattattt tcagccctga gttcctgcgt gaaagtaagg cactgtatga caatctgtat
     1021 ccttcgcgta ttattgtggg taccgatatg gatgatgagc gtttggttga ggatgcacac
     1081 acttttgcta cactgctaca ggaaggcgca atcaaagaga atgtggacac attgttcatg
     1141 ggctttactg aagcagaagc tgttaagctg tttgccaata catatctggc tcttcgtgtc
     1201 agctacttca atgagctgga cacttatgca gagatgaaag gcttgaacac tcagcagatc
     1261 attaatggag tttgccttga tcctcgtatt ggtactcatt acaacaaccc ttcctttggt
     1321 tacggtggct attgcctgcc gaaggatacc aagcagcttc tggccaacta cgcagatgtt
     1381 cctgaaaatc tgatcgaggc aatcgttgaa tctaaccgta ctcgtaagga ctttattgcg
     1441 gatcgtgtac ttcagatggc tggttattac aattacgcag aagcaaatca gtatgatgct
     1501 ggcaaagaaa agcctgttgt gattggtgtt taccgtttga ccatgaaatc caattctgat
     1561 aacttccgcc agagttctat ccagggcgtt atgaagcgtg taaaagctaa aggtgcaact
     1621 gttgtgattt atgagccgac cttgaaagat ggagagactt tctttggcag taaggttgtg
     1681 aatgatttgg aacagtttaa ggcaatgtgt gattctgtta tagcaaatcg ctacgatgct
     1741 tgccttgatg acattaagga aaaagtatat acccgtgata tcttccagag agactaagat
     1801 taggagagag tatttatgat tattacaaaa acaccgtttc gtatgtcttt ttttggcggt
     1861 ggaacggata tggaggatta tttcaaagag aatggcggtg cagttctttc tactactttt
     1921 gataagtact gttatgtgaa tgtgagacat ctgcctagat tttttgatta cagcgctgag
     1981 ttatcttatt ctcgtactga gagagtaact agcattgaag atattgagca tcctgcgatt
     2041 cgtaatgcga tgaaaatgct cgatatgcat gaaattcggc ttacatacga agctgattta
     2101 cctgctcgct ctggtctggg tacatccagt tcttttgcag ttggtatgtt gaatgctttt
     2161 tatgcactgg agggtaagta tgctgacaag aagaaacttg ctgatgaagc catttatctt
     2221 gaaagagttc tgtgtaatga agccggtgga tggcaggatc agattgccgc atcttatggt
     2281 gggttcaatc gaattaattt caatgtggat ggttatgagg tgctgcctgt cattatttcg
     2341 cctgaacgta aaaagcagct taacaacaat ctgatgatgt tctttaccgg attcactaga
     2401 ttttcttctg atgttcaaaa ggctaacaac gtcagtggca cagaggataa gagagtaaga
     2461 ctgaaaaaga tgtatgagct tgtagatgaa gctgaggcaa ttcttactga taagaacaga
     2521 aatttagatg atttcggtag acagcttgat gttacttgga gattgaaaaa aggtactggt
     2581 ggagcaatta gtacaggaag cattgatgaa ctatatgaaa agggtatggc tgcaggtgct
     2641 ttaggcggaa agttgctcgg tgcaggtggt ggaggtttct tggttttcta tgtacaacct
     2701 gagaaacagg atgcagttcg ttgggctatg cgtgatttga tgtatattcc gtttgaattt
     2761 gaaaatggtg gaactagagt tattcactac acgccggaaa cttatattga aaaacagaat
     2821 ggataaggag taacaatgtt agcggaaaaa cttaataaac atattgactt attgattaaa
     2881 cgatatccta cacttgagac gattaaacag gacattatcg atggatacct aattatggag
     2941 gaatgctatt tgaatggtgg taaacttctt gtggcaggaa acggaggatc agccgctgac
     3001 tcggaacata ttgctggcga gttgatgaag cggtttaaga cgccacgtcc tgtaagtaag
     3061 gaatttgccg ataagttagt tgcaattgac cctgtaagag gtccggaatt agccaaaaat
     3121 ctcgagtgtg gtttaacggc aattcctttg gtcgcacacg aagcattgtc tactgcttat
     3181 attaatgatg tcgatggttt gggtgtcttc gcacagcagc tgtttggctt tggtagatct
     3241 ggtgacgtat tccttggtat ttcgacgtcc ggaaatagca agaatattat gagcgcaact
     3301 gttgttgcta gggctctcgg tataaaagtt attggcttga cgggcgcaaa gggtggggaa
     3361 cttgccacgg tagcagatgt ttgtgttaag gctcctgaag ttgaaacata catgattcaa
     3421 gagtatcact tgccgatata tcattgttgg tgtttgatgc tggaagataa attctttgga
     3481 aacgaataaa atttggagga atgttaaatg aaaaagacaa ttatggttac cggtggttgc
     3541 ggtatgattg gctccaatct tgttaagaga ctggtgaagg aaggctgctg ggatgtttat
     3601 gtagctgata atctgtggag aggaaagttg gagtacctga acgacgaaga tgggcatcct
     3661 gtcatcgacc tggatactca tttcttcaat gctgatctga ctgattatga gcaggctaaa
     3721 aaggttattg gtacgactga gtatgtagtt catctggctg atgttgtggc tggcattgac
     3781 tatgtgttta agaaccaggg cgaattgttc cgaatcaata atctgatcaa ttctaatgtt
     3841 tttgattgtt gccgtaaggc tggcaaggag aaaatcaagg gtgttatcta tgttggtacc
     3901 gtatgctcct tccctctgac cagacagaat actttgaatc ctgagcctct ccgtgaagag
     3961 gagttgttcc ctgctcttcc tgagtctgct tatggctgga gcaaactgat gggccagttg
     4021 gagatggggt atttggaaaa agaaaccgga attccgtgct gtatcctgat gctccataat
     4081 gtttatggca cacctactga ttttggcgaa cgtagtcagg ttattcctgc tctgattcgt
     4141 aaagcaatca ataatcctga agagcctttt aatgtgtggg gtagcggaga gcagggacgt
     4201 gctttcattc atgtcaatga tatcgtaaat gcattggtgc tcgccctgga taagggttgg
     4261 ggtcatggtc atattcagat tggcccttct cactgtactt ccatcaagga aattgcatat
     4321 aagatcattg aaatttctgg caaagacatc aagcctttct ttgatacttc taagcctgaa
     4381 ggtgataagg ctcgttgcgc agattattct aaggcgaaag agattttgga ttgggagcct
     4441 actgtttctt tggaagaggg tcttcgtgaa tcctacaatt ggattgaaaa gcagattaag
     4501 ggttaataag tatataagct gttcttcctc cgtgtaatta gaggatgcgg aggaagaaca
     4561 tatgagtaag gatgtactga gatggaaaag aatatgtata aaagaaaacg tgatttcact
     4621 gtgtgtggaa tagtaggtgt ttgtgctact acaaagaacg agtttgtaag ggaagttttt
     4681 tccatgccaa catctccggt aagtgcgact gttgatctcg tgggagtgcc ggccattatt
     4741 tcggcaaagg aaaatcccaa aattgctgaa atgtatagcc aggctacaat ggctgcaata
     4801 gatggtatgc ctattgttaa aattggacgg cgcaaaggat ttacctgtga acgatgtgct
     4861 gcaccagata ttatgggaat ggtttttgaa gaaggcgtta gacagggaaa aacacattat
     4921 ttctatggtg gaaaagataa cgaagtgtta aataagcttc gtgcaaacct agaaagagat
     4981 tacccgggaa tcaaaattgt tggaatgtat tccccaccat ttcgtccgtt aaatgaagaa
     5041 gaggatagca agatttgcga ggaaatcaac aacttaaatc cagattttct gtgggttggt
     5101 attggtgcgc ctaagcaaga aatgtggatg tgggaacacc agaagaagat ccataacaca
     5161 gtaatgttcg gtgtaggcgc cggctttaac ttttttgcgg gcactttgga aaaagctcct
     5221 gaatggatgg aaaaagctag tctagagtgg ttatttagac tggtaaagga acctcgtaga
     5281 ctatggaggc ggtatatctt gggaggtata aagtatatct actatactat tgaatctaag
     5341 taggggggca ataaaaatag atgaaaatac ttatagtagt tacaatgacg aataactttg
     5401 gtaagaaggg gtcctataat tctcaggaaa taggattagc tcgagggtta gctgaattag
     5461 gccacgaagt tattgtatat aaaagtgtaa aaagtgatga ggaagttgga acagagcaat
     5521 taagcaatac ggcgatgatt cactatatca agtgtcgtta tgtaggatca aatggattct
     5581 tatcaacaaa gaattttgat aagaatgcgg atgttctaat acaatttgcg gaccttcaac
     5641 tatcagtacc tcatgtatac tcatggactg taaaaaatgg aataaaatat ataccttata
     5701 atgggataac gcgaagtcaa agtggcaata aatatataaa gacaattatt gaaatattgg
     5761 cgcatcgaaa ttataaaatt tataagaaaa atgggtgtgt tgccaaaaat gatcgagttt
     5821 ctgaaaaatt agtacaatta ggagtaaata aagtgaaact tgctcctgtt tgtgtggatt
     5881 ttgaacagtt acataagaat tacgaagcaa tagattttac tactctcaga gaaaaatgga
     5941 atattcctcg caatgttcgt gttattttat atgtaggacg gtttgaaaag gataaaagac
     6001 cgttagatat gattagttta tttaataaac ttgatgagca taattatttg ctgatggtag
     6061 gaactggtgc tttactcgag caaacgaagc agcagataaa tttacttgga ctgcagaata
     6121 gagtaaaaat aattgagcgt attcctaaca aagatatttg ggagttatat tgcttatcgg
     6181 atgtatttgt aaatctaaac tatactgaaa tttggggtat ggttttgcta gaggcaatgt
     6241 actatcgcct cgggattatt gctgttaatg cacctggccc acagaatatt attttggata
     6301 atagatatgg aatgttggct aaatctgacg atgaaatttt gacattctta aaagacggac
     6361 gatttaccaa cgatatcata gatgcagcat atcagcgtgt aactacagaa tttagatggt
     6421 caaagtcggg agaaattatg cttagcatgt tggaaggtga taaatgatat ggaagtaacg
     6481 aaattaatgg cattgaggaa tcgatatgct ttaaacatag ttgataattg cacgagaaaa
     6541 atagccaaaa ttcttggttg ttgtattggt aaaggtgcac agatagggaa ctctgttgaa
     6601 tttgttcata attctgtagg cactgtgatt cattctgaca caatccttga agatggtgtt
     6661 aaagtctatc agaatgtgac gtgtggttga gcagatagta atgaacgtat catcattcga
     6721 gtaaaaaaga acgctgtttt atgtgtgggt gctaaaattc tttgtaaatc taacacagaa
     6781 ttatgcgttg gggaaggtgc tgtagttgct gcaaatacag tggtattgag gtctattcca
     6841 ccctacgaag tgtggggggt acctgctaaa aagatgggga gataaaacat gaatgcatat
     6901 aaattataca aagtagaacg atggttttac ttacacaaaa tggagttttt tgccaagatt
     6961 gtaaaggcac taatttatgt acttcataat agctcaattt catatcaaac agaaattgga
     7021 gataattgta agttcttata cggtggcata ggttgtgtaa ttggaaaaga tacaaaaata
     7081 ggtaatcacg ttataatcgg aacgaatgta ctgactggtg gtagatccaa taagcctggt
     7141 atgcctacaa ttggaaacaa tgtatatatc gctactggcg caaaaatttt aggtaatatc
     7201 acaatagggg acaatgtaat agtaggggct aatgctgtgg tcattaatga tgttgaacct
     7261 aattgttcgg taggaggcgt tccggctaga gtattgaaaa aagatattga tgtaaatgat
     7321 tattgtagtt tgaatcgtta ctaaaaggag agattgtatg ttgtattttg ttattccttt
     7381 aatgagtgcg aaggcagcaa acaattggga gatggttagt accttattta accgaacatt
     7441 atggtcgtgt tataatcaga ctgatcctga tttcaaaatc attgttgctt gccatgaaat
     7501 tcctaaactc acgcatgaat atgatgacag agttgaattt attcaagtta gcgaaaagga
     7561 tgcccctatt cccacaaatc aagacgaaaa aatgattgat aagggttata aaacacatgc
     7621 tctcgcaatg cgtttgcgag agttgggcgg cggtttcgct atgatggttg acgcagatga
     7681 tcttgtaagt tctcatttgg cggaatatgt gaattcccat ccccaggaaa atggatttta
     7741 tgtcaaaacg ggatacgtgt actttgttgg agacgattat atgaaggttc taccaaagtt
     7801 ctcatcagga agtgcgtgta tagtaaaata tgcagtggaa gacctgcctg aggcatatcc
     7861 ggaagttatg acagcaaatt gtgatgataa tccatggatc attagaaaac gacatggggg
     7921 cgttgttcca gcatgtgaag agcaagggcg tccattaaaa cctcttccat tcaaagcagc
     7981 agtatatgta ttaggaacag gggacaatca ctcgctttat ggtaaaacga caaaatatca
     8041 gacgaaaatg cgagagatac tggaaatgtt tatagcaaaa cgtaaaatta aaggaaaact
     8101 gaaagcagaa ttttctgttg attggttata aggtgagtat tatgaaggta ttgatgtgtg
     8161 aaagccaaat gccagagaat tggtattcgt ttgatctttc taaagaactt aatcaatatg
     8221 ttgatttaac attgctgtgt aagcgtgatg ttcctccttt agatcctaaa attaagagca
     8281 aacatatttt gtatcataaa agtacaaata aagtgttttc ggcacttgtt tttctgaaaa
     8341 gcttgttctt aatttggaag gaaataagat ttggtgatta tgatattgtt catttgcaat
     8401 tgatgaaaag tctcaaatat gaaggcggat tgtacgtttt atgtaagaga cactataaaa
     8461 aattaattat gacagttcat aatgtgttac cacatgaggt tcgtgaaaag gatcgccaag
     8521 agcactttaa tatatattca aattgtgatg gtttcattgt tcacaatcga gtaacagaac
     8581 agcatttata tgctgaattc ccagaattaa atgcaaagtc atatgtttgt ccacatgcta
     8641 tctatggaga tgttaaacat atatctagag cgtatgataa agataagttt gaatttatct
     8701 tgtttggaca aattcgtcca tataagggaa ttgatgtact gcttaatgca atcacagaac
     8761 ttccaatgga tgtaagaaag aaagttcatt ttaccatagc aggtcaacaa catcctaaac
     8821 aggatagtac tgactataaa aaaatgattg aagatttaaa ggtttcagat tgtgttctat
     8881 ataagccaga gagaatttct gatgaagaaa tggacagtat gttcagaaat gcagatgccg
     8941 caatatttcc atataaagaa atctatggaa gtggagcgct tcttatggca tacacttatt
     9001 gcttaccaac tattgttagt aatgtacctg cttttatgga agaaaccgag gaaggaaaga
     9061 tagggttact tttcgaaaat ttgaattcta agagcctagc agacacaatt gtaaagtatg
     9121 tgaaccttag cccagctgag aaaagcaaaa tagaggatga tatatatgag cttgtaaacc
     9181 gcaagtatac ctgggcggca tctgcaaaac tcacatacga ggcatacaaa caaattttga
     9241 atgagtagta aaacaattag aaaggaataa taattaacat gcagattagt aaatggaaaa
     9301 tcatacagtt tctaatggtc tttctaatga ttatctcatc aagcatattg aagcctttgg
     9361 gcggctggtt gggagaaacg atgaacgtta gacttgtgac agtcattcta gcaatagtat
     9421 gtttgttaat agcctcatta ccagcattta gaaaaaatag atatttaaat ttctgcactt
     9481 tactgatttg tgttgttata atagcagctt acatctactc agataagaca atatgggtta
     9541 aatctaggga aagtatgagt ttatactatg tattattggc gtatccattg ttctgtactt
     9601 taattaacca agcgtggaat ttggattcaa tgctaaatac gatatgtggt gtaacatttc
     9661 tttcatacat tcttagaaca tctatttcac ttattcagaa attttcagga gtacttctct
     9721 atgaaaacat atatcgtgag tgtgccccgg aaaattggta tcgtggaggc tttttaagaa
     9781 ttaatccacc atgttttaca attataataa taccgattgc tttgtatatg attgagcgac
     9841 aagttgtagc tagaagaaaa gtaaaatatt atttactgat tgctagcgca ctggcatatt
     9901 ctgctgttat tcatggttct agatctgtcc ttgtctatca aattattgta cttggttgct
     9961 atatcctttt taagaaaggt tcttcgaaaa gaaaaattgc aatgtgggtt ttagctgggt
    10021 tgcttgtagt catatttatt aattcgacca tgttctcaac atttgttgaa actttcacaa
    10081 acagtacagg tgaatatgca ggatctgctg aatctcgatt tgcttcagtt tggtttttta
    10141 ttcctaaatt tttgcaatcg cctttgttcg gtactggtat tcttactggt gaggaattta
    10201 cgtttatatt accaggagga gttagaggac acctatctga tattggattt ttctatacaa
    10261 ttactcaata tggtgtaggt atacttatat ttgatattct gtttattata aaagggttca
    10321 aaacagcatt acttgcatca aaagtgggta tgactggcta tcaatattta gcatttggaa
    10381 ttacattaaa tgtcttgcta acagggatta atatagactg gttttatccg atatttactc
    10441 cagctattcc tgtatgctta gcggttatag aatacatcta tcaagaatgt agaagtgtgg
    10501 ctaacataga ataggaaata ttaataggag tgcatctcat gaataagaaa gagattttat
    10561 ataacatatt gaagaagtac gaaaatttac catatgtgtt taggaacatg tttggtcata
    10621 atataagtaa actcgtatct tggaaattga ctgctgattg tgcaacagta gaagaaaatt
    10681 ctgaagttta tgcagcgttt caattgcgct caaaggacat gtctgatgtt ccagtgatgg
    10741 gtttagttaa ttgttgcaaa gatgtagcaa ttgtaatgca ggggccacta tgtttaaagg
    10801 atgatttctc ttataatact ctggcctact ataagaaatg ttatccaaat gctttaatta
    10861 ttctttcgac atggaaggac gaagatatta aggcattgaa aaggatagag gatcttggag
    10921 ttatagttat ttgtagtgag aaacctgaaa agcctggaca cttaaatatt aattatcaag
    10981 ttggaaacac tttgcctgga atagtcagag caaagcagct tggtgcaaag tatgtttgta
    11041 aaactagaac agatcaaaga atataccatc caaatgcaat ggcgtttttc tgcagtttac
    11101 ttgaacagta tcctgttaat aacgaagatg attggaaatt aatacaaaat cagcgattac
    11161 tgttactatc aatgccgtat ggagacatgt ttttcccgta ttgtttgtca gacttcctgt
    11221 attttggtga tgttgatgat atgataaacc tcttctcgat tcctagtgat gtgagagaaa
    11281 agggagctgt gtctagagga gtctcaagaa gggagatatc tgaaaataat ttagctccag
    11341 aagttcagct tcttcgaagc tatatttcac gaatgggggg caatgaggag tgctctatta
    11401 gggcatattg ggagtttgta aaaaaccatg ttattactat taacaaaaat caaattgatt
    11461 tgtactggca taaatatgta ggtcgttatt caaataatac tatttatggt acgtattata
    11521 ttgatgatag ccaagatgca cttcactgct ataattttga ttttataaat tggttgaatt
    11581 tgtacactgg aaaatacgaa tacaaagcag aatatgaaaa atatatggac tttgtttggg
    11641 aagagtaatt aacttttata gtaaaaagta ctttagggaa gggcatatta tgaaaacagt
    11701 aattatggcg ggcggacgag gaagccgaat ttcggaattg tttccggata ttcctaagcc
    11761 aatgattcct atagatggca ttccagtttt agaacgagaa ttgtgttcgc ttagagatca
    11821 aggctttaat gatattattt tgactgtttc ctatttgcat gaaaaaatag aggagcattt
    11881 cggagatggt tcaaaattag gaattaaaat caaatatttt attgaaaaaa ctccacttgg
    11941 caatgcaggt gcattgtatt ttttgttgga tgaactgggt gatgaaccgt ttttactttt
    12001 gaatgctgat gcagtatttg atgttgattt caggcagatg attagatttc atgttgagaa
    12061 taacgcagat gttactttgt ttacgcatcc taactcacat ccgtatgata gtggattaat
    12121 tatatccgat gacaataaga aagtgcttca atggttagcg aaggaagatc aaagacctga
    12181 atattataaa aaccgagtaa atgcaggtct tcatgttata aatccgaagg tgttgagaga
    12241 aaatccatta atggaaattg taaataaatc aatagttgaa ggtggggggc taccatacaa
    12301 aattgattta gatagaatga ttctgaaacc attggctaac acagggcgta tgtattgcta
    12361 cgattctcca gagtatgtta aagatatggg aacaccggac cgatatagag ctgtaattaa
    12421 ggactttcaa aagggaatta ttgttggtaa aaatctatcc aatccacaaa aagcaatctt
    12481 tttagataga gatggaacga ttaataaata cgttggtttt ttgagaaatg cagaagaatt
    12541 tgagttaatt gatggagtat cggaggcaat taaaattatt aattctctag gttttctgtg
    12601 tattgttata acaaatcagc ctgtaattgc aagaggtgaa gttactgttg atgagcttaa
    12661 tagtattcac aataagcttg aaacaatgct agggcatgct ggtggatttt taaacggtat
    12721 ttattactgt ccgcaccatc cccataaagg atatgcagga gaggttatag aattaaaaat
    12781 tgattgcaat tgtcgcaagc caaaacctgg actgttatta gaagcatcaa aggattttaa
    12841 cataaaccta agtcaatcat ttatggttgg agatagtgaa agtgatgtaa ttgctggaaa
    12901 aaatgctggt tgtaagacag tactgattac agaagacatc accttgcctg ctaattatgg
    12961 ccaagactat agtgtctgtt cattgttaga atttgttaaa aatcatttat gcactatgca
    13021 ataattggtt tagtgaaact tgtaaaagga gtaattagat gaaggtatta attccagcag
    13081 caaaagttgt tgaaaaagaa ttgcagagta taggaatgtt accagcaatt atctatccaa
    13141 ttaataaaaa aattgtcttt gattatttgt ataatcagta taatgcaatc tgcgattcat
    13201 tggatattat ctgttatgaa aaagcggata aggttcatag aagactgaaa aactatcatg
    13261 attccagaat tcatatatgt gaccttcctg aattagggga tctaggacaa accgtgtatt
    13321 ttgggttatc agatgaagat gagcctgttc taattaactt cgcagataca attgttatgg
    13381 agagtatgtc tgatatagaa atggatacat tctattattc tgaagatttt gtgtcgagta
    13441 cttggacata ctttaaaatt aatgaaggaa taattactga tatccacgat aaagaggaag
    13501 cttacgtatc cgataaacgt gaaaaattat ttattggcgt atttatgttt tcttcgtcaa
    13561 aacttctgaa gagttgctta agagaggcag ttgagagtcg gaaacagggg ggaataaact
    13621 cattttatcg ggctttacag ctatacagcg aacgttgtcc gctaaaggcc gtaagaacag
    13681 aaaagtggtt tgatattgga cacatcgata aatattacaa tacaaagttg gaagttaaag
    13741 cacgagagtt taatcatatc tctatagata aagatcgagg gatattgaag aaaactagcg
    13801 atgacaagga taaatttatt ggagaaataa agtggtattt aaaacttcct gctgatattg
    13861 aatatgtaag acctagaatt tttgattatt ctacatctta cgtagctccc tatgttgata
    13921 tggaatatta tgcatatcat acggttcatg agttatactt atatggtgat ttaaaatata
    13981 accagtggca tgatctgttt agccgtataa agtttatctg tagcgatttt aagcgataca
    14041 ccttaaaaga tgaaaagatt agagagtcat tggaagatat gtatttgcga aaaaccatgc
    14101 agagatttca aaaattgtct gaagataaaa gattttcagc tttttttgag aaacaaattc
    14161 aaattaatgg tcgtaagttt atttctttaa ccggtgtaat tgaacttcta aagaatctta
    14221 tcccaagtat gctctatgat gtaaatgaat tttgtattat acatggagat ttgtgttttg
    14281 ctaatatgat ggtagattcc aattgttcct ttgttaaggt tattgatcct aggggaaaat
    14341 ttggaaaata tgatatttat ggggacagca gatatgaatt agcaaaacta tttcatagtt
    14401 tagatggtaa atatgattat atcatcaagg atctatttac tgtagaatat gataccgagt
    14461 gtgcagtgat agattataca attaatacgc gtgtaagaga ttacgattta tttgaattgt
    14521 ttaagtcggt cttttcggac gagattaagg acgattttga taagattgta ttgatagaag
    14581 cgttgctatt tcttagtatg attccattac acggtgaaag ttttgatcac caaatagtga
    14641 tgcttgctac aggtatggaa cttcttgcaa gagtaagtaa taaagttata ctctaatata
    14701 aattgtgcct taatgaataa cggttacaat actgttttag caatgtaggc attaaggcac
    14761 gattatatta attaatacaa tagaagggaa gagaaaaatg tatagtgaat attcttttgt
    14821 gtttgatatc gatggcactt tgtgtccgat aaagaagaag gatgagcgat atgaggattt
    14881 ggtgccgtat gagaatatgg ttgaaaaact gagattctat aaggagaatg gtgctaaaat
    14941 tacattgttc acctcacgca atgttaattc ctataaagga aatttgggaa tgataaatgc
    15001 taatactgca aaagtgctct tggcgtggtt ggataaatgg gacattccgt atgatgaaat
    15061 cctgtatggt aagccttggc cggggcataa aggcttctat gtagatgatc gaacggttag
    15121 acctgatgag tttttgagat gctctgtaga tgaattaaat gaaatctgca acagttcaaa
    15181 aaaaagattc taaataagga ggttagttct atggaaaaaa agatgacagt tatcattacg
    15241 atggcaggtt tgggaagtcg ttttcgaaaa attggttatg cggttcctaa atatatgatt
    15301 aatgccaaag gaagaacttt atttgattgg gccatggata gtttggttgg atacaataaa
    15361 aatgttgata agtatatttt tgttgttcgt caggaagaca acgcttcagc ttttataaag
    15421 gagcattgtg cttactttgg tattgaaaac gtagaagtga ttgaaataga ctatatgaca
    15481 gatggtcaag caactacatg tatgttggct attccttatt gcaatcaaga caatgagatt
    15541 atggtttaca atattgatac atacgtggaa tcaaatgaga tgaaatacga agatatttgt
    15601 ggagatggac atattccatg ctttcatgct gatggcgatc attggagttt tgcaaagctt
    15661 aacgatcgaa atgaagtaat agaagtccgc gaaaaagttc gcatttctga taattgtact
    15721 cttggtgcgt attatttctc atcggcaaag ctgtataaag ctttatatga agagtattat
    15781 gcagactcct ctaatatgga gaagaatgaa aaatatatag caccattata taactttatg
    15841 ataaaaaaag ggttaaaggt gactattagc atagtagatg cgaaaaaggt tcatgtgctt
    15901 ggtactccag aagagctcaa tgtgtttttg aatcaagaat aaatagtgtt taggagaaaa
    15961 tagcagtgac cagtaataat ctaattgtaa aaacattaag cagatataag aatactccac
    16021 ttcctgttaa ggctgcatta tggtttatgg tatgtggttt tttacagaga ggggtttcaa
    16081 tgattacaac cccaattttt actagagttt tgtctacaga acaatacggt ttatacagca
    16141 cttatctatc gtgggaatcc ttattgctca tgattacatc gcttagtttg ttcaaagcca
    16201 tgatgaatct ttatgtaaaa tatgataatc aagaacatat tctttcgaca gtatccggat
    16261 tgactttagt actgactatt ttttggtttg ttattgctat ttttattaag gatatattgg
    16321 cagaaatttt aggattgaca ccagttctag ttgtctgtat gttctcatat tttatatttc
    16381 aggcagaaat acaatgttgg tctttacaca aacgatatat atatgattat aagactttat
    16441 tagtagctac ccttgcagtt actgttggct catctgtagt tggctgttta gcggttctgt
    16501 ttatatctcc taccgctgag gcgagagcct taatgaatgt cctagtgact ggtgttgttg
    16561 gcgtggctgt gtatatatcg gtatttagaa gatgtaagga ttttaaggat aatgaagtgt
    16621 ggaatttcgc agtagtattt tgtgtatcgt tgatgccaca ctatttatct gaatttattc
    16681 tgaatggttc tgataaaatt atgataaatt acttatgtgg ttcgtcagat gttgctattt
    16741 acagcgtggc atattctatt ggtgctctaa ttaaccttgt tacaaattct gtcaattcca
    16801 cttttgcacc ttatcagtat caaaaaatca agtcaggaga atatgattta ctatcaaaaa
    16861 gagctaatga agttcttggt ttggtagcgg ctatgctttt tgcattaatg ctatttagta
    16921 aagaaattgt gttgatattt ggtgggcaga aatatatcga cagtgttaat ttaattgtac
    16981 ctatatgtat cggtatttat ttcaactata tgtttcagct gtttgctaga gtacaggaat
    17041 actttgaaaa gaaactcact gtcgttattc catcggtttt gtgtgcaatt atgaatattg
    17101 tactaaatta tgtttttata aagttatatg ggt
//
LOCUS       DANKHK010000053        16604 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000053 DANKHK010000000
VERSION     DANKHK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            101..1528
                     /locus_tag="PW109_001638"
     CDS             101..1528
                     /locus_tag="PW109_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511466.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1620..1985)
                     /locus_tag="PW109_001639"
     CDS             complement(1620..1985)
                     /locus_tag="PW109_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2511467.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1982..2347)
                     /locus_tag="PW109_001640"
     CDS             complement(1982..2347)
                     /locus_tag="PW109_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2511468.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2539..2787)
                     /locus_tag="PW109_001641"
     CDS             complement(2539..2787)
                     /locus_tag="PW109_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511469.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2786..2914
                     /locus_tag="PW109_001642"
     CDS             2786..2914
                     /locus_tag="PW109_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2511470.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2971..4665
                     /locus_tag="PW109_001643"
     CDS             2971..4665
                     /locus_tag="PW109_001643"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2511471.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4669..4788
                     /locus_tag="PW109_001644"
                     /pseudo
     CDS             4669..4788
                     /locus_tag="PW109_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4765..5061
                     /locus_tag="PW109_001645"
     CDS             4765..5061
                     /locus_tag="PW109_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511472.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5438..6571
                     /locus_tag="PW109_001646"
     CDS             5438..6571
                     /locus_tag="PW109_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2511473.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6602..6922
                     /locus_tag="PW109_001647"
     CDS             6602..6922
                     /locus_tag="PW109_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2511474.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6951..7415)
                     /locus_tag="PW109_001648"
     CDS             complement(6951..7415)
                     /locus_tag="PW109_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2511475.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7546..8799)
                     /locus_tag="PW109_001649"
     CDS             complement(7546..8799)
                     /locus_tag="PW109_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2511476.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8828..9145)
                     /locus_tag="PW109_001650"
     CDS             complement(8828..9145)
                     /locus_tag="PW109_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2511477.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9171..9491)
                     /locus_tag="PW109_001651"
     CDS             complement(9171..9491)
                     /locus_tag="PW109_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2511478.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9955..11370)
                     /locus_tag="PW109_001652"
     CDS             complement(9955..11370)
                     /locus_tag="PW109_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2511479.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11387..12703)
                     /locus_tag="PW109_001653"
     CDS             complement(11387..12703)
                     /locus_tag="PW109_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2511480.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12853..13551)
                     /locus_tag="PW109_001654"
     CDS             complement(12853..13551)
                     /locus_tag="PW109_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2511481.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13840..14796)
                     /locus_tag="PW109_001655"
     CDS             complement(13840..14796)
                     /locus_tag="PW109_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2511482.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14909..15742)
                     /gene="msrA"
                     /locus_tag="PW109_001656"
     CDS             complement(14909..15742)
                     /gene="msrA"
                     /locus_tag="PW109_001656"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2511483.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15917..>16604)
                     /locus_tag="PW109_001657"
     CDS             complement(15917..>16604)
                     /locus_tag="PW109_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511484.1"
                     /translation="LATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFK
                     NRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMD
                     YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVY
                     AYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC
                     LFFKIVFHKR"
BASE COUNT         5178 a         3336 c         2822 g         5268 t
ORIGIN      
        1 atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt
       61 tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga
      121 gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa
      181 tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa
      241 taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta
      301 cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa
      361 ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca
      421 attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt
      481 agcttatttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat
      541 ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga
      601 gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag
      661 tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt
      721 cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa
      781 acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta
      841 taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca
      901 ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac
      961 tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg
     1021 aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca
     1081 attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt tatataaaga
     1141 agtccagaca attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc
     1201 taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca
     1261 tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt
     1321 ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga
     1381 tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac
     1441 accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc
     1501 tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct
     1561 tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct
     1621 cacttatttt ggacatctac gggtccgatc tctttattta aaaaataaga aatgatggtt
     1681 cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat
     1741 tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc
     1801 tttttaatag cattgttctc gctgactcgg tcgctttgca cttttgtatg gacaaataaa
     1861 tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag
     1921 atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc
     1981 gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat
     2041 aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg
     2101 gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat
     2161 aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct
     2221 tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac
     2281 cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc
     2341 gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa
     2401 gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct
     2461 ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt
     2521 agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc
     2581 tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt
     2641 acctacccta tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc
     2701 tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga
     2761 aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt
     2821 aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga
     2881 cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg
     2941 acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt
     3001 gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg
     3061 gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac
     3121 actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt
     3181 gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt
     3241 cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag agattttgat
     3301 gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat
     3361 ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg
     3421 cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat
     3481 ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca
     3541 cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa
     3601 atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt
     3661 cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact
     3721 gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt
     3781 accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt
     3841 ttcactaatg ggtccattac agattcagct actttaggag attacgaaac accagctgta
     3901 gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac
     3961 aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt
     4021 accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa
     4081 ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa
     4141 gatgtcaata tgtctatcgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat
     4201 gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa
     4261 cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa
     4321 gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata
     4381 aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc
     4441 gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc
     4501 gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct
     4561 gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa
     4621 gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa
     4681 aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg
     4741 atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt
     4801 gaaaaagctg ccctgcatca agtaataaga acacccaagc taaaactgct tcttatgttt
     4861 ttgacttggt tggacttatt accgcagatg caagcttttg aacaccattt accaagaggt
     4921 tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg
     4981 tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
     5041 ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc
     5101 acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt
     5161 tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca
     5221 atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg
     5281 aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaag
     5341 cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa
     5401 tgcagacaat tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg
     5461 aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc
     5521 aatcatccgt atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg
     5581 tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga
     5641 agtgggatct caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat
     5701 tagttgtggt cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa
     5761 tccagatcag ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg
     5821 tggtcaagcg gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga
     5881 taatgatctt cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg
     5941 gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc
     6001 agtcggtcta ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt
     6061 agatccagta cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt
     6121 cttagaagac acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga
     6181 tgtcattatc gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa
     6241 tttagtaagc ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa
     6301 aaaatttgga acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct
     6361 gcaagaattt tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct
     6421 aatgccaaaa atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac
     6481 tcattcgctg ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga
     6541 taaaaatata aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt
     6601 tatgcccaaa atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga
     6661 aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc
     6721 tgtcgattta ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat
     6781 ccccgatcga tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc
     6841 tattctcaga atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac
     6901 aagcaaaaaa aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc
     6961 gtatcacttt gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat
     7021 gtccactcgt atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac
     7081 ccaagttgat gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta
     7141 cgcttttccg gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg
     7201 aagtcgtttt caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca
     7261 taccattgag gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca
     7321 acagcaggaa ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg
     7381 tgttctctta atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa
     7441 acgtttacaa aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta
     7501 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attgatcaag catctaaagc
     7561 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     7621 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     7681 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     7741 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     7801 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     7861 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     7921 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     7981 aaagaaaggt ttgttcacga taaacggtgc gctatgccca gctgcgattg cattaacgtt
     8041 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     8101 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     8161 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     8221 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     8281 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     8341 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     8401 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     8461 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     8521 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     8581 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     8641 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     8701 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     8761 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     8821 ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa
     8881 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     8941 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     9001 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     9061 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     9121 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     9181 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     9241 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     9301 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     9361 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     9421 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     9481 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     9541 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     9601 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     9661 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     9721 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
     9781 tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc acaacctctc
     9841 aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc
     9901 tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt
     9961 atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc
    10021 agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg
    10081 attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc
    10141 gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc
    10201 gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt catattctct
    10261 tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat
    10321 ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt
    10381 cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca
    10441 ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac
    10501 tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt
    10561 ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac
    10621 tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg
    10681 agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg
    10741 gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca tcatccactt
    10801 ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac
    10861 gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat
    10921 ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc
    10981 caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc
    11041 gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt
    11101 ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat
    11161 ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg
    11221 gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc
    11281 ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag
    11341 aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat
    11401 tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac
    11461 aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga
    11521 tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg
    11581 caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg
    11641 gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag
    11701 caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa
    11761 tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca
    11821 tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg
    11881 ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca
    11941 cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac
    12001 cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt
    12061 ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag
    12121 cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct
    12181 tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa
    12241 ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat
    12301 tttcattcac taattcataa acagttggat taacagataa ataagacatc actgcgatga
    12361 taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa
    12421 cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta
    12481 attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca
    12541 ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg
    12601 cgttttttat caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa
    12661 cattggctaa tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt
    12721 gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta
    12781 ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca
    12841 tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt
    12901 tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc
    12961 ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt
    13021 tttattaaac aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt
    13081 tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt
    13141 gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt
    13201 cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc
    13261 aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt
    13321 ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt
    13381 cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc
    13441 ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata
    13501 tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac
    13561 ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga
    13621 ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac
    13681 cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac
    13741 aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt
    13801 ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat
    13861 tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa
    13921 ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca
    13981 tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt
    14041 gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg
    14101 tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag
    14161 cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc
    14221 tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt
    14281 tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat
    14341 atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg
    14401 tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg
    14461 gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc
    14521 ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa
    14581 atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt
    14641 attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg
    14701 attatggata ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa
    14761 tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc
    14821 aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt
    14881 tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt
    14941 gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt
    15001 tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg
    15061 taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt
    15121 tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac
    15181 gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc
    15241 aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag
    15301 cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc
    15361 cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca
    15421 tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg
    15481 catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa
    15541 agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta
    15601 gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca
    15661 atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa
    15721 tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact
    15781 ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt
    15841 ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta
    15901 taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg
    15961 cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa
    16021 tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct
    16081 ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga
    16141 ttacttggct cttctcatta gttactctat atatgataga actaccagtt gttttataag
    16201 gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct
    16261 ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg
    16321 cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga
    16381 aatttttttt actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa
    16441 atagttctct tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag
    16501 aagagaatat tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg
    16561 tattttgcag aagataccat ggcaccaaac tagttgtagc tagc
//
LOCUS       DANKHK010000054        16296 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000054 DANKHK010000000
VERSION     DANKHK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..1127)
                     /gene="queG"
                     /locus_tag="PW109_001658"
     CDS             complement(<1..1127)
                     /gene="queG"
                     /locus_tag="PW109_001658"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2511485.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQ"
     gene            complement(1146..1898)
                     /locus_tag="PW109_001659"
     CDS             complement(1146..1898)
                     /locus_tag="PW109_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511486.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFIPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(2038..2988)
                     /locus_tag="PW109_001660"
     CDS             complement(2038..2988)
                     /locus_tag="PW109_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2511487.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKGKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(2989..3828)
                     /locus_tag="PW109_001661"
     CDS             complement(2989..3828)
                     /locus_tag="PW109_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2511488.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(3825..4571)
                     /locus_tag="PW109_001662"
     CDS             complement(3825..4571)
                     /locus_tag="PW109_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2511489.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4809..6179
                     /locus_tag="PW109_001663"
     CDS             4809..6179
                     /locus_tag="PW109_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2511490.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAMNGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTRK"
     gene            complement(6234..7130)
                     /locus_tag="PW109_001664"
     CDS             complement(6234..7130)
                     /locus_tag="PW109_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2511491.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVKDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDPSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7244..7414)
                     /locus_tag="PW109_001665"
     CDS             complement(7244..7414)
                     /locus_tag="PW109_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511492.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7411..8088)
                     /locus_tag="PW109_001666"
     CDS             complement(7411..8088)
                     /locus_tag="PW109_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2511493.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGNSL"
     gene            complement(8179..9084)
                     /locus_tag="PW109_001667"
     CDS             complement(8179..9084)
                     /locus_tag="PW109_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2511494.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9259..10713
                     /locus_tag="PW109_001668"
     CDS             9259..10713
                     /locus_tag="PW109_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2511495.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVT
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            10729..11760
                     /locus_tag="PW109_001669"
     CDS             10729..11760
                     /locus_tag="PW109_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2511496.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTQDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENTTGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLDKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11767..12954
                     /locus_tag="PW109_001670"
     CDS             11767..12954
                     /locus_tag="PW109_001670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2511497.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13110..14492
                     /locus_tag="PW109_001671"
     CDS             13110..14492
                     /locus_tag="PW109_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2511498.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14464..14850
                     /locus_tag="PW109_001672"
     CDS             14464..14850
                     /locus_tag="PW109_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511499.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15046..15714)
                     /locus_tag="PW109_001673"
     CDS             complement(15046..15714)
                     /locus_tag="PW109_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2511500.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTESWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(15903..>16296)
                     /locus_tag="PW109_001674"
     CDS             complement(15903..>16296)
                     /locus_tag="PW109_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2511501.1"
                     /translation="SYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMK
                     AYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYI
                     YYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         4668 a         3289 c         3053 g         5286 t
ORIGIN      
        1 ttctgggcat gttccaattc gtttataacg acttcttctg tttctttagc taatatttca
       61 tctaatacat caagccattt ttccggttct tttgttccta gacgtccaat agcccaagct
      121 gctgttcctc tgatgacagg gcggacatct gttaaacatt ctgaaatctc aggcaatgcc
      181 gttcgatcac ctagattagc taaagcaatc aatgcgtttc tctgcaaagg tttcttccca
      241 cgccatgaac cagctaaatg cccaaattgc tgtttgaagt ctttattaga catcgaaagc
      301 attggcttta attttggata tacttcgtct acttttggtt ccatctcttc atgaaaatga
      361 aaatcttttc cttgattgta agggcagact aactggcaga tatcacatcc ataaatgaca
      421 tttcgcattt ttttacgata ttcttccggc atcatccctt ttgtctgagt ttgataagac
      481 aaacatcgct gggcattcat tcttccgtca ccaagcaatg cttttgtcgg acaagcagtc
      541 acgcagcgca tacagtcacc gcaaccaaat tctcctggtt tatcaggctc aaatacaata
      601 ttagtaacga tctcaccaag atagacaaat gaaccgaact cttcagtgat caacaatccg
      661 tttcgtccaa taaatcctaa accagctcgc tgtgctacag cgacatcgat cagttctcca
      721 gtatctactt gcggagctag acgccattcc tcttcttttt gccatttttc tgctcttgtt
      781 tggatgaatt caatcaatcg gttcaaacgg tctctgagta tatcatggta atcgatcccc
      841 caagaagcac gagcaaactg tccgcgtttt tcatccctag gcacttcttc atggatccta
      901 gtaggatacg caagtgcaat ggaaatgatc gatttcggct gatcaaatgt catttcagga
      961 taaagacgct catcgatatt tggatgttca aagccagaag tatggccagc tgctttttgt
     1021 tcttccaacg attcttttaa tgaatcaaaa ggttccgccg tggtgaaccc gattttgtca
     1081 attcctaatc gtttgctttc ttgtatgatt tcttctttta atgtcatggt cttcctcgct
     1141 tgctgttaac ggctgtataa cccataatat agtttttctt ttttcagctg gacaaactgg
     1201 atctgctgat cggcaatctt cttttcttcc atccattttt ttcttgcagc aaatacttcc
     1261 caataatttg ttgaaattaa ttttcttttg ttatctaatg cattaaaaaa attcaatgaa
     1321 actggttttg tacttaacag ttcatggagc cttacctctt cccatggcga aaactcagac
     1381 agatcgcggg aatacaatac ttcttcatag agattttttt gctgtgtgta cgtgaatgtt
     1441 tgcgggattt tcccaggcct ggcaatttct actagacgat tataaaacat attcattaat
     1501 ttgctgttgg caataaattg acttttataa taggccgact ggatcttttt gattactcct
     1561 tctgctttga tgactgaaaa taatgcaggc catgctttga cggttatttc gtcgttttgt
     1621 ttaactaata cgccaggtat gaagccgact ggagaaagga acacaaaata accgataccg
     1681 gtattttcat ctaaaggctg aaattcatca aacaaccatt tgcctatgta cagctcttca
     1741 tctggttctg ttgtctcatt ttgatttgct atgaataatt tagctaattc tgcttcaaga
     1801 aattgaaggt cttgatcctt gtcggtattt tcctgctggc tgatcaatcg aaagacttct
     1861 tctttaatcg tatatttatc tttacgtgta aaattcatag ccacaccttc cttccttact
     1921 ctaactataa cggaatttct tcaaaaaagg tatctgtacg ccgaaaaaca gcataatttt
     1981 tttcatatct cttataaaaa atgcaaggcc tgctcctcaa gctagacctt gcacttttca
     2041 gctttctttt atttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
     2101 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
     2161 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
     2221 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
     2281 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
     2341 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
     2401 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
     2461 tgcatttttt ttatatagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
     2521 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
     2581 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
     2641 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
     2701 aagattcaaa ccgttgtata aaataacatc cgcatcgctt gcctttttga tgtcttctgg
     2761 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
     2821 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
     2881 ctttccttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
     2941 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
     3001 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
     3061 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
     3121 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
     3181 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
     3241 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
     3301 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
     3361 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
     3421 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
     3481 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
     3541 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
     3601 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
     3661 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
     3721 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
     3781 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
     3841 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
     3901 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
     3961 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
     4021 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
     4081 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
     4141 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
     4201 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
     4261 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
     4321 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
     4381 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
     4441 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
     4501 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
     4561 gtacgttcca aatcttacac tcctttttag gttaacctaa ctttttattt aaatatagtt
     4621 taactaaagt ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
     4681 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
     4741 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
     4801 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
     4861 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgct
     4921 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
     4981 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
     5041 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
     5101 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
     5161 ctgatttgcc ggcaattatt cccgggatca ttgtcgtctt gtttcttatg ctggcaaatc
     5221 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
     5281 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
     5341 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
     5401 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgattg
     5461 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
     5521 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
     5581 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
     5641 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
     5701 attcagggat tttcagcgca agtcgtatgg tctatacact tgccatgaat ggaaagatgt
     5761 ctaaaaaatt tctgaaactt tctcgtcacg gcgcgccttt ttatcctgtc gtagcaatct
     5821 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
     5881 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
     5941 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
     6001 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
     6061 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
     6121 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc cgaaaataaa
     6181 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
     6241 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
     6301 aagattgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
     6361 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agagggatct
     6421 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgccgtctca
     6481 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
     6541 tgcaaataaa tatcggaaat cttagctaca tgattcgtat aattagccga taattgattg
     6601 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
     6661 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
     6721 gataaaccag ctatgccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
     6781 gcaggcgtct tttcttcgat ctttttcgaa taatctttta cgattgcttg aagttgctct
     6841 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
     6901 tcacttgttt gtttcgtctc tgttttcttt gtcgtggttt ccgttatggt cgtacttttc
     6961 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
     7021 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
     7081 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
     7141 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
     7201 tgccgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
     7261 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
     7321 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
     7381 catctaattg ttggatcagc caatcatttg tcataatgaa tttcctttca tttcttgcag
     7441 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
     7501 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
     7561 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
     7621 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
     7681 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
     7741 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
     7801 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
     7861 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
     7921 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
     7981 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
     8041 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
     8101 tttttagtct ttgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
     8161 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
     8221 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
     8281 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
     8341 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
     8401 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
     8461 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
     8521 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
     8581 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
     8641 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
     8701 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
     8761 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
     8821 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
     8881 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
     8941 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
     9001 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
     9061 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
     9121 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
     9181 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
     9241 aggagaggaa tacagattat gaaaagcaaa atgatccaac gcgtgtccta cgctttaggc
     9301 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
     9361 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
     9421 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
     9481 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
     9541 gttagttccg tactcttggt aacaatcttt accaatttct ttggcttagc tactcataat
     9601 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
     9661 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
     9721 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
     9781 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
     9841 gcttcaggct ggttctggtt cggtgtgatt gctgctgtga tttcaggcgg tactgcgatt
     9901 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
     9961 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    10021 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    10081 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    10141 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    10201 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    10261 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    10321 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    10381 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    10441 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    10501 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    10561 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    10621 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    10681 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    10741 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    10801 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    10861 gttttgggat ttgatacagc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    10921 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    10981 caattagcca cagatccaaa aactgggcat aatctgcatg gtggtgctcc tggatttgaa
    11041 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    11101 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    11161 actttgacac aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    11221 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcaatcaatc
    11281 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    11341 cctatagggg taaaagaaaa tactacagga agtgctttcg attttcaaat acctaaaaaa
    11401 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    11461 ccatttttct taaaagaaac cggcttggat aaagaagccg ctcgcttgac tagccccgat
    11521 aaaagaatcc aagtagatat tgctacagat gcttctagtg tggttatttt cactgcaaat
    11581 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcatgg tggcatcaca
    11641 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    11701 aaagccggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    11761 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    11821 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    11881 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt cgcagcacct
    11941 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    12001 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    12061 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    12121 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattggta
    12181 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    12241 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    12301 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    12361 atggtcccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    12421 gaattggcag attcaaaata caatgaacgc cgcagcgaat gcgaagaagc cttggcacgt
    12481 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    12541 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    12601 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    12661 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    12721 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    12781 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    12841 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    12901 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    12961 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa ttttcaaatg
    13021 tgagcctttt gctcgtattc gaaaattgct gttttttatt gacacagaaa gagaacatgt
    13081 gttctaattg tattgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    13141 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    13201 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    13261 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    13321 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    13381 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    13441 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    13501 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    13561 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    13621 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaaacaaa
    13681 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    13741 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    13801 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    13861 gtactcagct ttatgcccat gcttggggga tcgatcgaag ttttttggga caagaatata
    13921 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    13981 aagaaatcga gatcgttatc aaagaaatgg ccgatcaagt cggcactcgc ttgagacgat
    14041 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    14101 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    14161 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    14221 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    14281 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    14341 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    14401 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    14461 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    14521 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    14581 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    14641 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    14701 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    14761 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    14821 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    14881 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    14941 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    15001 ttagttttga gtaacaatcc aaggcgctga tttcacaacc tctgtctatc catctattcg
    15061 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    15121 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    15181 ccatgattca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    15241 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    15301 attctctagg ataacatgtt tggcattttc aagagaaaag cattcctgct gtcttaagat
    15361 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    15421 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    15481 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    15541 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    15601 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    15661 taataataaa ccgatcaacc cgaataccag tttcattgta attgaaaaaa tcattttttc
    15721 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    15781 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    15841 aagttagact tttttggagt ggagaaatcc actccttttt ttcatgctct aaaatgatat
    15901 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    15961 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    16021 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    16081 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    16141 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    16201 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    16261 gctaatgcat tgggcttttc agagatacga taggat
//
LOCUS       DANKHK010000055        16149 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000055 DANKHK010000000
VERSION     DANKHK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(249..1976)
                     /locus_tag="PW109_001675"
     CDS             complement(249..1976)
                     /locus_tag="PW109_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2511502.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1969..3162)
                     /locus_tag="PW109_001676"
     CDS             complement(1969..3162)
                     /locus_tag="PW109_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2511503.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3349..3993)
                     /locus_tag="PW109_001677"
     CDS             complement(3349..3993)
                     /locus_tag="PW109_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2511504.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4087..4221)
                     /locus_tag="PW109_001678"
     CDS             complement(4087..4221)
                     /locus_tag="PW109_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2511505.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4223..6355)
                     /gene="feoB"
                     /locus_tag="PW109_001679"
     CDS             complement(4223..6355)
                     /gene="feoB"
                     /locus_tag="PW109_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2511506.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6352..6825)
                     /locus_tag="PW109_001680"
     CDS             complement(6352..6825)
                     /locus_tag="PW109_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2511507.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7226..7450
                     /gene="nrdH"
                     /locus_tag="PW109_001681"
     CDS             7226..7450
                     /gene="nrdH"
                     /locus_tag="PW109_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2511508.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7609..9768
                     /gene="nrdE"
                     /locus_tag="PW109_001682"
     CDS             7609..9768
                     /gene="nrdE"
                     /locus_tag="PW109_001682"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2511509.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9818..10783
                     /gene="nrdF"
                     /locus_tag="PW109_001683"
     CDS             9818..10783
                     /gene="nrdF"
                     /locus_tag="PW109_001683"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2511510.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10872..12011)
                     /locus_tag="PW109_001684"
     CDS             complement(10872..12011)
                     /locus_tag="PW109_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2511511.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12320..13033)
                     /locus_tag="PW109_001685"
     CDS             complement(12320..13033)
                     /locus_tag="PW109_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2511512.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13287..13988)
                     /gene="nagB"
                     /locus_tag="PW109_001686"
     CDS             complement(13287..13988)
                     /gene="nagB"
                     /locus_tag="PW109_001686"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2511513.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14222..15754
                     /locus_tag="PW109_001687"
     CDS             14222..15754
                     /locus_tag="PW109_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2511514.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15945..16124)
                     /locus_tag="PW109_001688"
                     /pseudo
     CDS             complement(15945..16124)
                     /locus_tag="PW109_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4996 a         3340 c         2832 g         4981 t
ORIGIN      
        1 ttaatatttt ttgagaatgc acttcgtaca taataaactt taatcacatt agtctttcat
       61 acctacgatc gatctctttg actcgcaccc ataaattcat aatagtttgt cggcgtcttt
      121 gaacgtttcg aagatgagct acgcattctc tttttttata gacaaaaaag atagagacga
      181 acatggagct tccaaatttg gatatttgat ccaattttgg aagctccatt tatttcgtgt
      241 ctgtcttttt attttttcca gctttcgact aattcttggt tctctttgat ccagtctttt
      301 gctgcttgct gtggatcttt tccattgttg atttccaaca taactttttc catatctttt
      361 tctgaccaat ggaaattgtc taatactttg taagcttctg gctggtcttc tttcaatcct
      421 tttcgaacca tcgtatggat ctgctcttcg cttcccattc cgttttcagg atcttctaag
      481 tatttcagat catatttggc aaacatccaa tgaggtgtcc atcctgtaac tacaattggt
      541 tcgtgttttt tgatcgcttg acctagtgca acggtcattg ctcctgaaga agatgtctct
      601 actttccagt ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc cgtaactaca
      661 ccagctcctg gctcgattcc tatgattttc ttacctgcct gatcggttaa ctcatcgatc
      721 gaatttacat ccatgtaggc tggcactgct aaaccaactt ttgctcctgt caaattggca
      781 cctaggtctt cgacttgatc tttatattgt gcatattgtg aaccatgagt tttaggtagc
      841 caagctgaaa ccatggcatc tgactcgcca tttgaaacag atttccacat gattgaatta
      901 tctaatggcg tcatcgttac atcatagccc atttgtttca atacttcacc gactacattt
      961 gttgaagcaa cttctgtatc ccattcaaca taagataagt tgatacgttt tgtttctttg
     1021 gcagatgaaa aactccctat accaatcgca cctaagatca ctactactac agcaccaatg
     1081 atcagtcctt gacgtttttt acttttctgt gttgttgctc ctgcagcttt tttattgttt
     1141 ggctggttca aatgctgtgt aaaacggtca atgatgattg ctaagataac taatgcaacc
     1201 ccgttgacaa atccattccc gacttgtgct ctttgcaatg ctgataatac gccgcgtcct
     1261 aagccgggtg caccgatcat agaggcaata acgaccattg aaagtgacag cattgttgtt
     1321 tgattgatcc ctgccatgat cgtactttta gctaaaggca attctagttt gaatagtttt
     1381 tgacgacttg tgctcccgaa tgaatcagaa gcttctacta attctttagg aacttgtcga
     1441 atccctaaat tcgtaaaacg aacggttggt ggcagagcaa agatgactga ggcaaatacc
     1501 cctggaacca tcccgatacc gaaaaatgca actgctggaa tcaaataaac gaatcctggc
     1561 attgtctgca taaagtcaag gatcggtttg ataatacttt gggctttact gctttttgcc
     1621 atcaagatcc ctaatggtac cccaatgatg atcgagatca cactagataa aagaacaagt
     1681 gtgacagtat tcatcaaatc attccataga ccttggttat aaataaacca tagaccgatt
     1741 aatgtgaaca acgtaagtcc tggacgtttc ttagaaataa agaaagctgc taccgtcaat
     1801 aacaagataa acaaaggagc tggaatcact aacagtaaat ttgtaatccc cgacatcact
     1861 gcctgtccaa tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt aaaccattct
     1921 gtgatatttt ctacccagtt tgccactggt aattgatagt tcaatagatt attcatgttc
     1981 actcacctcg cttgtttctg ctaatgcttc aatcacacta cctcggatga cgacaccttt
     2041 taattttcca tcctctacaa ctgcaagtgg agtcggtgag tcataaataa tattgaaaat
     2101 atcacttacc accatgtctt tcccaatcac cgtcacattt tcatctagga agtcgatcaa
     2161 tggacgtttt tgcttcctag cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg
     2221 tctctttctg tcaacagcca tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc
     2281 aacgtttggt ccatcagatt cgatattcgt cgtcaatgca ggtaccatga tgttttgagc
     2341 cgtaagcact ttggaacgat cgacttcttc cacaaattca cgtacataat catttgctgg
     2401 attggtcaat atttcttcac ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc
     2461 gattctgtcc ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt
     2521 ttgaacattg gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga tcagcggatc
     2581 aagagccgag aacgcttcgt ccatcagcaa gatctcagga tcatttgcta aagctctggc
     2641 taggccaacc cgttgttgca tcccaccaga taactgacta ggatattggt ctttaaatga
     2701 aagcaaacta gaattttcta atgctttttc tgctttttct tgtcgttctt ctttaggtac
     2761 accacggact tctaacccgt attctgtatt ttccaagatc gttcgatgtg gaaacagacc
     2821 gaagttttgg aaaaccatat tgattttatg acgacggact tctcgcaatt cttctttgct
     2881 taacttcgat acatcttctc catcaatata gatactgccc gaagttggct caatcagacg
     2941 attcaataga cggatcaatg tggatttccc actcccggaa agccccatga tcacgaagat
     3001 ttctccttct tttacgtcaa aattcacgtc atacacacca accgttgcac cggttttctt
     3061 taaaatctcc gttttcggct ggtgatcatt catcatatct aaagcggctt gtgtcttttt
     3121 accaaatatc tttgttaaat gttttacttc tacttttacc aaacaaattc tcctttttta
     3181 tgtaaattca tttttctttc tgaaaaaagt gaccacacta tgttaacatc atgtagtcac
     3241 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
     3301 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
     3361 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
     3421 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
     3481 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
     3541 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
     3601 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
     3661 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
     3721 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
     3781 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
     3841 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
     3901 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
     3961 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
     4021 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
     4081 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
     4141 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
     4201 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     4261 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     4321 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     4381 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     4441 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     4501 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     4561 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     4621 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     4681 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     4741 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     4801 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     4861 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     4921 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     4981 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     5041 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     5101 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     5161 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     5221 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     5281 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     5341 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     5401 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     5461 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     5521 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     5581 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     5641 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     5701 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     5761 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     5821 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     5881 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     5941 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     6001 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     6061 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     6121 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     6181 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     6241 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     6301 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     6361 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     6421 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     6481 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     6541 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     6601 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     6661 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     6721 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     6781 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     6841 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     6901 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     6961 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     7021 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     7081 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     7141 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     7201 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     7261 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     7321 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     7381 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     7441 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     7501 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     7561 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     7621 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     7681 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     7741 ccaaatacga tgacttttcc atctattatg gaaaaaatca attacttgat cgatcaagaa
     7801 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     7861 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     7921 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     7981 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     8041 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     8101 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     8161 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     8221 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     8281 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     8341 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     8401 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     8461 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     8521 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     8581 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     8641 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     8701 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     8761 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     8821 acagatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     8881 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     8941 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     9001 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     9061 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     9121 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     9181 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     9241 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     9301 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     9361 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     9421 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
     9481 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaacg
     9541 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
     9601 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
     9661 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
     9721 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
     9781 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
     9841 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
     9901 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
     9961 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
    10021 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
    10081 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
    10141 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
    10201 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
    10261 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
    10321 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
    10381 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
    10441 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
    10501 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
    10561 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
    10621 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
    10681 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
    10741 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
    10801 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
    10861 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
    10921 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
    10981 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
    11041 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
    11101 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
    11161 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
    11221 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
    11281 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
    11341 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
    11401 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
    11461 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
    11521 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
    11581 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
    11641 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
    11701 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
    11761 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
    11821 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
    11881 attgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
    11941 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
    12001 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
    12061 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
    12121 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
    12181 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
    12241 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
    12301 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
    12361 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
    12421 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
    12481 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
    12541 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
    12601 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
    12661 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
    12721 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
    12781 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
    12841 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
    12901 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
    12961 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
    13021 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
    13081 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
    13141 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
    13201 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    13261 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    13321 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    13381 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    13441 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    13501 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    13561 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    13621 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    13681 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    13741 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    13801 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    13861 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    13921 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    13981 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    14041 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    14101 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    14161 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    14221 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    14281 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    14341 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    14401 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    14461 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    14521 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    14581 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    14641 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    14701 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    14761 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    14821 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    14881 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    14941 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    15001 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    15061 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    15121 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    15181 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    15241 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    15301 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    15361 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    15421 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    15481 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    15541 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    15601 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    15661 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    15721 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    15781 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    15841 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    15901 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    15961 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    16021 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    16081 gaaccagccg cacctccaat aaagtacata aacataaata cagacccgtg gtgctagtta
    16141 attttaata
//
LOCUS       DANKHK010000056        16129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000056 DANKHK010000000
VERSION     DANKHK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..180)
                     /locus_tag="PW109_001689"
     CDS             complement(<1..180)
                     /locus_tag="PW109_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511515.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESI"
     gene            complement(202..1539)
                     /locus_tag="PW109_001690"
     CDS             complement(202..1539)
                     /locus_tag="PW109_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2511516.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     DFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGISPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKKHSVETYRFAKNQ
                     RIGYKLLDSEGGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRHL
                     GKSLNGRADSRINWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIEIVLDV
                     FGENKDQFFGFAVEEEPLKYKKKCVDLVYKKVVYDISESLSTDYDQFNVHDTKPALER
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETAREIDYQKRQNQYDMDF"
     gene            complement(1524..2020)
                     /locus_tag="PW109_001691"
                     /pseudo
     CDS             complement(1524..2020)
                     /locus_tag="PW109_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2169..2507)
                     /locus_tag="PW109_001692"
     CDS             complement(2169..2507)
                     /locus_tag="PW109_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511517.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKFAYEKTGISSVKEFE
                     ETFEINQKGTREKWQKAVNFANSEFTLVHEFLASDQLLETKFQQFLRRKGETAYRIFY
                     REETQEESVR"
     gene            complement(2511..2696)
                     /locus_tag="PW109_001693"
     CDS             complement(2511..2696)
                     /locus_tag="PW109_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511518.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNELMNELSHIQKGA"
     gene            complement(2710..5400)
                     /locus_tag="PW109_001694"
     CDS             complement(2710..5400)
                     /locus_tag="PW109_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511519.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ERAIKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQGAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKLVKENVQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGT
                     KTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQSSSASQPKTVDVR
                     ENVQSSTSSQGFKTVDVRENAQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS
                     EIRENDKGSKPRRRFTYGDNELFRDTLNDPNPLFDSLLKK"
     gene            complement(5599..6564)
                     /locus_tag="PW109_001695"
     CDS             complement(5599..6564)
                     /locus_tag="PW109_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDL2511520.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(6754..7038)
                     /locus_tag="PW109_001696"
     CDS             complement(6754..7038)
                     /locus_tag="PW109_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511521.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(7051..7344)
                     /locus_tag="PW109_001697"
     CDS             complement(7051..7344)
                     /locus_tag="PW109_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511522.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            7413..8177
                     /locus_tag="PW109_001698"
     CDS             7413..8177
                     /locus_tag="PW109_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511523.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            8228..9373
                     /locus_tag="PW109_001699"
     CDS             8228..9373
                     /locus_tag="PW109_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2511524.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(9458..9763)
                     /locus_tag="PW109_001700"
     CDS             complement(9458..9763)
                     /locus_tag="PW109_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2511525.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(9788..10219)
                     /gene="ruvX"
                     /locus_tag="PW109_001701"
     CDS             complement(9788..10219)
                     /gene="ruvX"
                     /locus_tag="PW109_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2511526.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(10216..10482)
                     /locus_tag="PW109_001702"
     CDS             complement(10216..10482)
                     /locus_tag="PW109_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2511527.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(10737..11462)
                     /locus_tag="PW109_001703"
     CDS             complement(10737..11462)
                     /locus_tag="PW109_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2511528.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(11823..11893)
                     /locus_tag="PW109_001704"
     tRNA            complement(11823..11893)
                     /locus_tag="PW109_001704"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11859..11861),aa:Gly,seq:ccc)
     gene            12010..12762
                     /locus_tag="PW109_001705"
     CDS             12010..12762
                     /locus_tag="PW109_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2511529.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            12778..13257
                     /locus_tag="PW109_001706"
     CDS             12778..13257
                     /locus_tag="PW109_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2511530.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(13508..15088)
                     /locus_tag="PW109_001707"
     CDS             complement(13508..15088)
                     /locus_tag="PW109_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2511531.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(15081..15824)
                     /locus_tag="PW109_001708"
     CDS             complement(15081..15824)
                     /locus_tag="PW109_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511532.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(16000..>16129)
                     /locus_tag="PW109_001709"
     CDS             complement(16000..>16129)
                     /locus_tag="PW109_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2511533.1"
                     /translation="ITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         4891 a         3191 c         2467 g         5580 t
ORIGIN      
        1 aatgctttca gaatcattaa atttattatt tgatggacta gctaacactg gtaaatttga
       61 aaccataaca cgaaacatat ttgccttgga accaaaaact cctttgtttt ggttgtaaaa
      121 attgttgaat atttctttag tttcttctga agccgagact ttaaaatatg gatagttcat
      181 tttttctctc tccctgttta ctcaaaaatc catatcatat tgattttgtc gtttttgata
      241 atcaatttct cttgctgttt cttcatactc tctccgagct ttttcaatcg taccatcatc
      301 aattgcacga ttaaataaat aaagaacacg ttttgtttct tgaatataac gcttcttaaa
      361 attacgttct aaagcgggtt tagtatcatg aacgttaaat tggtcataat cagttgataa
      421 actctctgag atatcataaa caactttttt atacaccaaa tctacacatt tcttcttata
      481 ctttaaaggc tcctcttcca ctgcaaatcc aaagaattgg tctttattct caccaaaaac
      541 atctaataca atttctattc tgtcctctat ttcaaattta tatttgtcat aaaattcttc
      601 ataatctttt gatgatctaa gaaatggtat attctcatta tctaattcaa agcgttgcgc
      661 tcgttcttgc caattaattc tactatccgc tctaccattt aacgatttac caagatgtcg
      721 aatagcaatt tcagtgagta cctcttcaga ttgaatttgc tccgcttttt tattttctgt
      781 gaacaaatca tcaagataat tcatatcata gttttgccct aaacgctttc cgccaatctt
      841 taagattaac ccaccttcag aatctaagag tttgtaccca atacgctgat ttttggcaaa
      901 tcgataggtc tcaacagaat gttttttctt caattccgca tgaaagtcat cataattttt
      961 cgcatactga gcaacttcag aaacagctag ttttattttt tctcgatttg aaatcttatt
     1021 ttcagttcta aattcacgtt ctttccaatt ttcacgatct gcaactaaat gattatactt
     1081 atacatatca agtgggctaa ttccattatc aataagtata ttatcattcc aggcctgcaa
     1141 aactcgaata tcattctttc gaatatccat tttctttcct gttcgctgat cgtttggtga
     1201 aataccaaag tgattgtgaa catgcccttt gtctgtgtgt gtgacgacca aaattgactg
     1261 gttaggaaac attttttctg cccacttaac acccaactca tgtgcttttt cagcggaaat
     1321 atcttctagt ggatcaaagc tttgtgtaaa atgccaaagc tttattttcc ctttatcacc
     1381 aaaaatttca ttggttcgtt taaaatcctc aatagcattt aaatcattcg atacgccaat
     1441 tcctgtcacg agttcagaat cttcttgttt atttaacata tatgaaattg tattatgaat
     1501 actcctactt tttgaagcaa tttttataat cgagcccata acttattcaa ctctttttcc
     1561 aagcgattca atggatcact tattgatttt atttgtatca ttaagtcatt tttttagcat
     1621 atagccgctt taagcgctct tgcttagttg taaaagataa gtaaacactc tgatgttctt
     1681 ctttttgtat ctcggtatct tttttattta actgaacacg caaataattt gctgttttca
     1741 ctaattgatt caaatttctt cctattgcta gtaaatgaaa attaatttct ttatattgtc
     1801 gatcggtata ttttagactt ttccctgcgt tgtctactgc gattttcttc acgtacattg
     1861 gaacagataa accaaaaatg tctgctgttt tttctagctc ttggtactga taatcgtcta
     1921 ctcgaaactt tatcggccta ttttgtcgtc gactatcttc attacgttta tatcgctttc
     1981 gcctttcttc taatggtagt ttactgacat caattgccat gcgcacttct cctttccaat
     2041 attattttta agttcaaaag aagcaagcga ggggaaaggg aacccttccc cgcttgtcga
     2101 gggagtaaac tccctacgag caccctcatg ctccgcattt tatcgagatt agatagtcga
     2161 taaatcagct acctaacact ttcttcttgc gtctcttctc gatagaaaat tcgatacgct
     2221 gtctctcctt ttcttctcaa aaattgttgg aacttcgttt ccaacaattg atcgcttgca
     2281 agaaattcat gcactagggt aaattcgcta ttcgcgaaat ttaccgcctt ttgccatttt
     2341 tctcgagtac ctttttgatt aatctcaaaa gtttcttcaa actcttttac tgatgaaatt
     2401 cctgtttttt cataggcaaa ctttcccagt tcgggataaa gctttctctc caactcggcg
     2461 agttcttttt tcaaatctgc aattgatttt tgttttttcc ttgccatttt tcatgctcct
     2521 ttttgtatgt gactaagttc attcataagc tcatttttca tctttaattc ttcttgaatc
     2581 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgctttctt tactgattca
     2641 cataattcgt caagttctaa gaaatcatgt gtactaatct gtagaattaa ttgcatgaaa
     2701 atcacctctt tattttttaa gtaaactatc gaaaagtgga ttaggatcat taagtgtatc
     2761 tctgaataac tcgttgtctc cataggtaaa tcttcgtctt ggttttgatc ccttatcatt
     2821 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     2881 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     2941 ttgagcgttc tcccgaacat ctactgtttt gaacccttga ctacttgtag aactttgtac
     3001 attttctcgg acatctactg tttttggttg acttgcactg ctactttgca cgttctctcg
     3061 gacatccact gatttaaagc cttgactact tgtagaattt tgtacatttt ctcgaacatc
     3121 tactgttttt ggttgacttg tactgctact ttgcagattt tctcgaacat caattgtctt
     3181 agtaccttga ccatttgaag aactttgtaa attctctctt acatcaacat tccgtccgac
     3241 atgaccattc aatgaacctt gaatattttc tcggacatct actgatcggc caccttgact
     3301 acttgtagaa ttttgtacat tttctttgac caatttttct aatccggtat ttgaattttt
     3361 agcttcttgg attctttctt tggtcaacgc ttctttttcg tccatattag cgcctgtacg
     3421 cccttctaca cgtttcctta acatttcctc acgattcatt gcttcaggcg tgtatttcgc
     3481 tccctgtcgc ttctgattaa ttaattcttc agcagactta gcttgaccgt gcatagcatt
     3541 tttacgttct tcaatatctt gttgaacttt ttgctgaatt gcacttacag gattattatt
     3601 cgattcagca ttatttttca ttgcttcacg catacgttca gaactagaca taattctatc
     3661 ggcattttca accttagcac catcttttct atccgaaaga cgtgttggca tggaatcagc
     3721 atattttcca gtttcatctt ctttattttt agctaacata ggtttgctat ttttattaat
     3781 agcggacacg gtaccatcat ctaaaccttc tttgaactta ttcttagtat cattagccat
     3841 atcacgcata ctcttaaatg gttttattgc tctttcacta gccattgtag ctccatctga
     3901 agctaaacct gataatccac gttcatgggc atatcccaaa gctcttccag ttttgccagc
     3961 tttttttgat agagtttcgc cgaaattctt ttctggtttt cgatcttctc cttctccgct
     4021 gtctgatcca tttccatttc cgcttccttt agcacgatta aatccatttg atccttttct
     4081 aaacagcatt gcgcccatac caaaagcaga agcaagttgc ccataacctt ctttgagtcc
     4141 aatatcaaca ccaaagtatc tcatgactgt tccactgcct ttgattaaaa caaaaacggc
     4201 acagatataa gcaattgttt ttataaaaat atttgttgag ataccttgtc ctgagttcag
     4261 aaaattgata aacatagcaa acatggatag tccaaatcct tgaaagccta cagtcaaata
     4321 gcactgtaga atatctgata gaacaacttt tgaacgctgt cctgtttcta agtcagtagc
     4381 aaatactaaa actccgagta cacgtttaaa tgctaactcg agtattgcag taataatcac
     4441 aaaggcgcta aaaacatagg ctattgctaa agctatcagc ccaattgaaa tgccccatag
     4501 attcgcttga tagcgataat atcctgattt gataccatca acaaaggttg acaaaaaatt
     4561 atctgaaaat ttagtagcaa caaattcatt atcagaattt tcaactaatt cataacgcag
     4621 gttatcagct gttgagttct ctttttccat tttatcaatt acattatctg tcaatacttg
     4681 cgtaagatcc gttttccaaa aattttcttt tgttagattg ttccgatcat tcgttttatc
     4741 gatcatggaa tattgatccg ttttattgat ataagctaaa tcagtaaccc cttgttcaat
     4801 cagcgaccat gccacaccgc catcttgatt gttaatacta gttgcgtcat tataaagagt
     4861 ctttgcaaac attattcctg aaagtttttc tgttgatcca atcaaagtcg gcattaaaag
     4921 aattaatact atcgacatta aaacattgat tccaacagat tttagatcaa ccgttccttt
     4981 tccagtcacc attttataac caataatcac tagtgaaagt atcattaagc caacaacaat
     5041 agtgcttaaa actgactgat agactttgtt caatccagca ctttccacaa agctaaacaa
     5101 atctaacact tcaggaatca tattttgaat accatctaca attgcaaaca tcgattttat
     5161 aacagaccac cctatccaca atccaaattg actgtaaaaa tttccgttat atttttttaa
     5221 atacggttga tagacaggat tattcaaaaa ttcagtttga ctttccgtat catcgaacca
     5281 attaaaaatg cttgcatgag caacattacc ggtgaaaata acgataaaca ttaaagcaag
     5341 taagctcaga gctaaccagt ttattttcct accagcaatc ttttttttaa ttattttcat
     5401 tgttacctcc ctcagcccat ttttttaggc tctcagaaag cttctgagcc atttcttgat
     5461 taattatgat atgagtttac acaattgtca ttttcagctc ttaaatcgag aaccacggtg
     5521 ttaaagcaga tactctcgat taattcgaga gtatctacat tttgatcgag acagcagatt
     5581 tggtctatta gacgattttc aatcatataa cacctctctt gtttgagtaa caaagttatt
     5641 atatgctctg gaacttacag tatttttgca gttatataaa gttgtaatcc agtaaagatt
     5701 gagattgtgt atctcatgat caattgcgtt aaatgaatca atagcagatt caatatcaaa
     5761 ttgattgagt tgttctactt tttctatcac ttcatttgca gatttttctt ctctatttat
     5821 cctgtatgtt ttgctctttt cagagtatat tgattgaatt atattaacaa tatcttcaac
     5881 aattcttttg taacaaggat tggattttag cgcattatat ccaatttgat tctttatttt
     5941 ttgtctcata atttcgactt ccatgtcatc cgatagattg atagattgat cttccctaat
     6001 ctcacctaaa ctaaccttac ctgacctact ctgatctgaa ctaacctgac ctaatctaac
     6061 ctgggcatgc ccatggtcgt cctttggtag accaacggga taccattttt caatcaaatc
     6121 atttcgaagc atttccgttt gacctgcttt taaaattttt tcaatactaa tttgttttaa
     6181 tcttgtaaaa aagtctggat tactatttaa taatttaaat cgatatatag agggtgtata
     6241 tcttgtagtt cttaacgttt gattttgcag ccaatgggga agaaatacta ataaatcttc
     6301 attcagcata tagagaaatt ctttagctaa aagaattttc acgtcatcat caggggttcc
     6361 aagcatccgt acatatggat atgcttcgac aatcccatca tcgtctgcgt ttacattcag
     6421 atatacatat aataattttg ctgaatccga catcttcata aaactcgcat tatttaaaaa
     6481 agtttttgac aacatacgtt tttctgccat aaattccaca cttcctcctg tcaatgttaa
     6541 aatgtagtca aataaagttt tcacagaact ttattattga gtgttcttcc ggtctgcaaa
     6601 ctgtaaggag cactcttttt ttgtatattc accttttatt aaatcaaata gaaaataaat
     6661 cttgatttct tttaaactca actttgcttc ttcatcatcg ttcaaacaaa cacttccttt
     6721 ttttcatatc aaaagcacac cacgcaactt tttttatatt tcatattctt tgataaatct
     6781 tgctaactct tcttttctga taaattgaca gtctccgatg atactcacct ttaaaccacg
     6841 ctgattaatg aattttttta gtgtacttct actacaactc aaatattcac acgcttctcc
     6901 aagccggaaa atttctttgc ttaaagatgt ctcaatacga acttgatcaa cagcttcctt
     6961 agcaacttga taaaaatatt tacgtaatga ttgatcaaga tctggcggta gaataacttg
     7021 tacactcgat acatccatga tgcccactcc tcattcgtct tttttcgtct tcaaatcact
     7081 aaaaaaaata tccacaatat ctaaatgaat tccatcttta tgaaatagct ttacgattct
     7141 ttctatttca taatctttaa acggcaccct tcctttctct ttgttacgat acgttagttc
     7201 cgatatttct aataactccg caagttcttt ttgtctatat ccacagaacc tgcggtattt
     7261 tctaactgga gagtccaacc ttaccacctc gtcttttttc gtctttacca acgatagaat
     7321 agcatttatt tcattgtccg tcaatactta cccgtcgttt ttcgtagatt ccattccgtt
     7381 tttgtgttac attactgtcg gagggatata atatgaacct taataagaca tcagtaggaa
     7441 accgtattcg tttagttcgt aaagaactcg ggctttccat gaaagagttc ggaaaaaaga
     7501 taactcctac cgccgcagat agtattgttt cgcgttggga gcgagggatt agcttaccta
     7561 ataacgacag gctaactcaa attgctaaaa ttgctcataa agatattaac tggttattat
     7621 ggggaacttt taaagaattt atttcatcat tgttagataa agaaggatac acaaattttt
     7681 tgaatgattt tcctgaaaca gtagatacta tttataatga tttaatgcct atttcggaaa
     7741 atttacagtt tcctgattac gaaaattcta ttttacgact attcaataag atatacttac
     7801 ctatattcaa tgattactgt ctagacatta ttgaactgga tttggaggac tttataaata
     7861 agagacattc catctcctct gacaagttta aaaataggtt tttatcctcc tttcatgaag
     7921 gagtatctaa cagcggatat aaatacggcg atacggattt aatttataaa tatggaattg
     7981 cattacttga gaaatttgac gattcttata aattaaataa tccatatgag tcaatagatg
     8041 cgtatttttc tgccatgact gtaaacactg aagataccga aaaacttata tataccattt
     8101 gtaaatcgct tggcattcca ttccaaaaag gaagcaatac cgcacagatt ctttcaaaaa
     8161 accacaataa tttttaattc attttttcta tcaaaagcac gccacgcaac tgataggagg
     8221 aaaaacaatg gcaacaatta agcaatatga aaagaaagac ggaaccaaag cttggctatt
     8281 tcagacctac cttggaatca gtccattaac tggtaaagaa gtccgtacta cacgtagaaa
     8341 ttttaaaaca aaaaaagaag ctcaaatcga gcttaataga ctacttgtag actttgaaga
     8401 gaacggactt aaaaaggaaa caaacgaaac tttcaaagaa gtgtatgatc tctggtatga
     8461 gagctataaa actactgtaa aggaagtcac tcaattaaaa attgagatta agtttaataa
     8521 atggattctt ccaaaatatg ggcagtttag aattaaagaa attactacaa aacatgttca
     8581 gaagatattg aatcagtggg ctaaaacaac agatcaatat aaagtcctac attctactgc
     8641 aagccgtatt ttcaaatatg caatgacttt agggataatt actaaaaacc cctgtgaact
     8701 tgtaatcatg cccaatagaa acgttgaaaa aaagaaagat actgtcaaaa tttattcacg
     8761 ggatcaacta gaaagtctct tccaatactt ggaatcaaaa agaagcacct atagagccga
     8821 ctacgataaa acgctacttc gctttctatt ttttagtggt tgtcgaatta gcgaagcttt
     8881 agctttagat tggtcagata ttgactttga ttcaaacacc gtcactataa acaaaacctt
     8941 aagccaaaca aaatatgggt acaaaatttc aagtccaaaa acgagaaata gctatggttc
     9001 tctatcttta gacaataaaa ccattaattg gctaaagaaa tggcaaatca atcaaaaaag
     9061 atatatgtta acccttggta ttactgatcc aacaatgatt ttttgtggaa tctacaagga
     9121 aatcattact aaacacgcta tttattctcg tatgataaca atctgcaaga attctggtat
     9181 tccatttcta ggtaaccatg taaccagaca cactcatgct agtatgctgt tagagtcagg
     9241 agtctccatg aaggaagttc aagaacgcct tagacactca tcaatcaaat taacaatgga
     9301 tacctatgct catctaacta aagaaaccaa agaaaaaacc gttgaaaagc ttgcagagca
     9361 cctaaatttc tagcggaacg tattcaatct cgtattcaat tcaaaataca aaaaagctga
     9421 acccttatct accaagggtt cagctaccat ttaaagctta ttcttcttct tcagccatga
     9481 atgtattgaa tacttcttca atcatgtccc attctgcgtc tgtttcgatt tgttctaact
     9541 cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct acatcttcat
     9601 cttctgaaac accagcagga tataaaagaa catagttacg tccaaattct tcttgtccgt
     9661 caactgttag taggatttca tataatgttt catttccttg gtcatccacc aatgtgatgt
     9721 gttcgtgtcc ttcatggtcg tgggaatggt tatgttctgt cataagatta cctcttttct
     9781 tttattatta aaataattgc ttacctgagc cgtctaaata attttgtaag atcatcacag
     9841 ctgctaattt atcgatgact ttttttcgtt tggctcttga agtatttgcc tgctctacta
     9901 gcatacgttc tgcctgaaca gtggttaatc gttcatcttg ataatcaact ggcaaggaga
     9961 atagttcttt gatcttttct ccgtatgaca tggaagcttc tgcccttggt ccgatggaat
    10021 tgttcatatt tttaggtaat ccaactacaa accggtctac ttcatattgt gctactaatt
    10081 ccttcaaccg atcaaagcca aactcttctt ctgcttcatt gatccgaatg atctctactc
    10141 cttgagctgt ccagccaaag gggtcactga tagcaacacc tactgtctta gaaccgacat
    10201 ccaagcccat tactctcata aattcactcc atgatttgag aggtagtcct tgacaattac
    10261 ttccatgatc tcatcgcgtt cgtgacggcg aatcaaattt cgagcatcac gataacgagg
    10321 aatgtaggcc gggtctccag aaagcaagta gccgacaatc tgattgattg gattgtagcc
    10381 tttttcttcc aaagcccgat aaacaatcgc taatgtttcg ctcacttctt ttttgcgact
    10441 atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc atgattctac acctctttcc
    10501 cgcaaggtaa ttccagctta ccttctacaa ccttatttta ctcgaagaaa gggaaaacta
    10561 caaacaaata ttccaaactt ccgaataaat taagaaacac tatttgtatg tttttttcct
    10621 tttttctgta gctttatcaa aagtactctt ttcataaagt atacgctata tttctttgaa
    10681 tgtatgtttt tacacaattt tttcataata ttcataaaaa attcgaatag ctgctactaa
    10741 ttttttgttt ttgttgcttg tcctgtctca taattgatcg tgtagccatc ttgaacattg
    10801 tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc caattgcact
    10861 cccctggcta ccagttcatt cccctcaaaa tgaggagtga cacggtagcg tacatgatga
    10921 tttgtttctt tgatatatgc cgcaatatca ttttcgtgag caagcatgta tggagcattc
    10981 aacgaacgtg tccctgtcat caagttttta atgttgttat tttgaccagt cagctggaaa
    11041 ccaattaaat ggctgcgatt atataaccat tgcccgtcac ttaatttctt ttgtttccaa
    11101 cctgtcgggt caatatacaa aggctctctc ttttcagtag ggaacaaatc ttttccaagc
    11161 atagcattgg cttgtcctac tctgttcaat ttatcaatat ttgaatagct ttcccatgtt
    11221 cctttggaaa ggctaagatc ctcttttgta aattccggct gattcccatt gatttctatt
    11281 tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg tggtgtggaa
    11341 tcaaaaatag ttgtaatctg ctgaaagata tctgaatctt tttgtgtggt ggaaacaccg
    11401 aatgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt ttttttgctc
    11461 atttctttac tcctttacta acagctccct aaatcttaac atatgttccc ctataaataa
    11521 atactagaaa gaaatagaaa cagcaaaaaa taaaacctgt tttaagatgg cttaaaaata
    11581 caaaaaagag acggattcct cgtctctaat cagtcaccac tctatctgta aggtatttat
    11641 tattattcaa aaattttctt catactcaag acaatacact gatttcacaa ccgcttggta
    11701 aacggtgcgc aaaagcttgc atctgcggta ataagtccat ccaagttaaa aacataagaa
    11761 gttgtttttg cttggctgtt cttattactt gttgcagagc agctttttca caacctattt
    11821 taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga ttttaccact
    11881 aaactacacc cgcaatacaa aaacagtata cgcctctttt attccttcct caagtttttt
    11941 tattctttac gcttctggta tgttttgtag taaaataaaa ctaatcaatc aattattgga
    12001 gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt actcgctggg
    12061 aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat gagtcgaatt
    12121 gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg tctctttatt
    12181 ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc gattaattta
    12241 gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga gctaacatta
    12301 gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt tcctgtctgg
    12361 ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt tggcggaaag
    12421 atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc ttatctatat
    12481 agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt attgattggc
    12541 atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct aatcattatc
    12601 ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg tgatttgcta
    12661 gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac tgctacgatg
    12721 atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa cacaagtatg
    12781 gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt tgccgttatt
    12841 acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt tggctggatg
    12901 atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt gcttggttct
    12961 ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt
    13021 ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca agcggttcga
    13081 aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc aattatgatt
    13141 gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa catccgtaat
    13201 ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt taaataaacg
    13261 aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc
    13321 tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga gtattaaaaa
    13381 aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata ctgagaaact
    13441 gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt gtcacggccc
    13501 ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc catagagcgg
    13561 aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga taaaacaaag
    13621 ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc atcatagttg
    13681 cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg gtccagttca
    13741 gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag cagccgaata
    13801 aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac agtccgctta
    13861 ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg aacttttctt
    13921 ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt tcttgatcca
    13981 aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc aatgcgatcc
    14041 ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt atttcgcctc
    14101 ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa agaacttgca
    14161 taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc tgatgcacat
    14221 tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca gtcagttgct
    14281 ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga gccgtgatag
    14341 ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca gcatcataga
    14401 atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata gtccccgttt
    14461 gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca agcaagatac
    14521 tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca aaaacaatca
    14581 aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt gagataatga
    14641 tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg actagaagcg
    14701 gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt
    14761 cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa aagatattat
    14821 aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg taataagtaa
    14881 agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa atcagtaaaa
    14941 aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc tttccctttt
    15001 ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga ttaactcgaa
    15061 ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg ccttccagcc
    15121 attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa ttcactaact
    15181 gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca taactgttgt
    15241 gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc atgagccttc
    15301 atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat
    15361 acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca
    15421 tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag cgctgtcaat
    15481 tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga aatcaagtcc
    15541 caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt
    15601 ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa aatcgttccc
    15661 gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc ggcaccatta
    15721 tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc ttttaatgct
    15781 tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt actccttttg
    15841 tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca aaaagagtgc
    15901 ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt ctacaggaaa
    15961 acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt cattttctat
    16021 cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct ctagttgttc
    16081 tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatg
//
LOCUS       DANKHK010000057        15751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000057 DANKHK010000000
VERSION     DANKHK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..343
                     /locus_tag="PW109_001710"
     CDS             <1..343
                     /locus_tag="PW109_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2511534.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTV"
     gene            350..709
                     /locus_tag="PW109_001711"
                     /pseudo
     CDS             350..709
                     /locus_tag="PW109_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            981..2027
                     /gene="gmd"
                     /locus_tag="PW109_001712"
     CDS             981..2027
                     /gene="gmd"
                     /locus_tag="PW109_001712"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDL2511535.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            2107..3120
                     /locus_tag="PW109_001713"
     CDS             2107..3120
                     /locus_tag="PW109_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511536.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            3157..4101
                     /locus_tag="PW109_001714"
     CDS             3157..4101
                     /locus_tag="PW109_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL2511537.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            4169..5482
                     /locus_tag="PW109_001715"
     CDS             4169..5482
                     /locus_tag="PW109_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2511538.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            5566..6468
                     /locus_tag="PW109_001716"
     CDS             5566..6468
                     /locus_tag="PW109_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDL2511539.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            6473..8125
                     /locus_tag="PW109_001717"
     CDS             6473..8125
                     /locus_tag="PW109_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2511540.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            8149..9015
                     /locus_tag="PW109_001718"
     CDS             8149..9015
                     /locus_tag="PW109_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511541.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(9057..9248)
                     /locus_tag="PW109_001719"
     CDS             complement(9057..9248)
                     /locus_tag="PW109_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511542.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            9278..9817
                     /locus_tag="PW109_001720"
     CDS             9278..9817
                     /locus_tag="PW109_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511543.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9945..10298
                     /locus_tag="PW109_001721"
     CDS             9945..10298
                     /locus_tag="PW109_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511544.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            10766..11242
                     /locus_tag="PW109_001722"
                     /pseudo
     CDS             10766..11242
                     /locus_tag="PW109_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            11242..11436
                     /locus_tag="PW109_001723"
     CDS             11242..11436
                     /locus_tag="PW109_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511545.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            11442..11727
                     /locus_tag="PW109_001724"
                     /pseudo
     CDS             11442..11727
                     /locus_tag="PW109_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            11741..12107
                     /locus_tag="PW109_001725"
                     /pseudo
     CDS             11741..12107
                     /locus_tag="PW109_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            12321..12719
                     /gene="tnpA"
                     /locus_tag="PW109_001726"
                     /pseudo
     CDS             12321..12719
                     /gene="tnpA"
                     /locus_tag="PW109_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12880..13101
                     /locus_tag="PW109_001727"
     CDS             12880..13101
                     /locus_tag="PW109_001727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2511546.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(13411..14638)
                     /locus_tag="PW109_001728"
                     /pseudo
     CDS             complement(13411..14638)
                     /locus_tag="PW109_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(14742..15692)
                     /gene="yidC"
                     /locus_tag="PW109_001729"
     CDS             complement(14742..15692)
                     /gene="yidC"
                     /locus_tag="PW109_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2511547.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSITGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
BASE COUNT         4565 a         3109 c         3751 g         4326 t
ORIGIN      
        1 catcacgaga catgtttggg gagatctgat ggatgaagtg gaacatcttc gcctaacaga
       61 tctgaataaa tcaatttaca aaaaacggaa acaaacgatt gaacgaatat ttgcggatgc
      121 aaaagaaaag catgggatgc gttggaccaa gtatcgaggc ttggaaaaag tcgcgacgca
      181 tacgatgcta gtgtttgctg caatgaattt aaagaaatta gccacctggc tatggaaagg
      241 caaggagcct ttattttttt gttcaaaaat tcgaaacgaa gtagataaaa agctgttcca
      301 agctcgagtt acgagtttgg aacagctttt gtctacagtc tgacaacctc tgacagaggt
      361 tgagattttt catactgatc gtggaaaaga gtttgataac caagctattg atgaattatc
      421 aacaactttt gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt
      481 agctgaatca acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac
      541 cttacaacaa ttggatttgg agttatttga ctatgtcaat tggtggaacc gccttcggtt
      601 gcacggtaca cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg
      661 aatccttgat aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt
      721 agctcctgcc gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc
      781 atacggaaag aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccaatt
      841 cacgaaaaac taatattgtg ctaatgcgtg cgtagattgt tctatgaaat atcgaaaatt
      901 atcgtgtatt cttgaaaagt attagaaatt acgagccatt tatatggcct tatcacttac
      961 ataggaaagg ataatgtatc atgaaaaaag cgctgattac tggtattact ggtcaggacg
     1021 gttcctatct ggcagatttc ctgctagaga agggctacga agttcacggt atcactcgcc
     1081 gtgcttctat ttccaatacc gctcgcattg accatttgat gggcaagatc acccttcatg
     1141 acggtgacct gtccgactca tcttctctga ttcgtatcat cagcattgtt cagcctgatg
     1201 aaatctataa cctcgctgca cagagccatg ttcaggtttc ttttgatgtt cctgagtact
     1261 ccggcgatgt tgatgctttg ggcgttatgc gtattctgga agcttgccgt attctgggcc
     1321 tgacgaagaa gactaagatt tatcaggcat ctacttctga gctgtatggc aaggttgaag
     1381 aagttcctca gcgtgaaacc actccgtttc atccttacag cccttatgct gttgcaaagc
     1441 agtatggctt ctggatcact aaggaatatc gtgaagctta tggcatgttc gctgtcaacg
     1501 gtattctgtt caaccatgaa tccgaacgtc gcggtgagaa tttcgtcacc cgtaagatca
     1561 ccctcgctgc cggccgcatt gcagaaggcc tgcaggacca tctggaactg ggcaacatgg
     1621 actccctgcg tgactggggc tatgcaaagg actatgttga gtgcatgtgg atgattctcc
     1681 agcatgagac tcccgaggac ttcgtgatcg ctaccggcga gcagcacact gttcgtgact
     1741 tcaccgagaa ggcattcgca gcaaatggca tgaccattcg ttgggaaggc actggcattg
     1801 atgaaaaggg ctacgatgtt gaaaccggca agatgctcgt ttgtgtaaat ccacagtggt
     1861 tccgtccgac tgacgttgac aacctgtggg gcgacccgac taaggcaaag accgttctcg
     1921 gttggaatcc gcagaagacc tcttacgagg aactggttaa catcatggca aagcatgacc
     1981 gtgaattggc aaagcgcgaa gctgcaatga agaacgcagt caagtaagct gacattgaat
     2041 aaacatatct gtttctatct ttccagtcac ggcttcggac atatcgcgcg gaatctgccc
     2101 attattatgg caacactctg ccgatgcgaa gcgaagatta cactggtttg cggcgcaaat
     2161 catctggatt ttgcaaagcg caatttggag caggaactgc ccaaagagct gatagaccgc
     2221 gtgcgctatc gtccaataaa cacagatatc ggtctgatta accgtgaggg caccctactg
     2281 gtggacacgg gtaagctgac cgagatctgt gaatattttt tggcaaaggt tccttctttg
     2341 gcaaagacag aagccgaatg gctcaagcga gaagctgtca gcgcagtgct ttgcgatatg
     2401 ccaatctggg caatggatgc ctgcgatatg gcaggagtac ctttcctgta tgtaggaaac
     2461 ttcacttgga cagagctgta tcgcgaattt ttacccgaac atatttggaa agaatatgcc
     2521 aagcgttacg gtaaaattca gcacaccatg ctttatgcgc taaataacga agaaatgttg
     2581 gaatttctcc aaaacagcga aaaaacaccg acttccgttg tggcgcgtcc gattcataac
     2641 gacgaagtgc aacgcattaa atcacagcat gaaaaaccgc ctgtatttgt cgcactcggt
     2701 atgtccgcgc ggttcaaaca gccgattgat gtcagtcacg cgccgtatga tttcattaca
     2761 acgctaggtg ttccgcttgt gggagaaaac gtaaccgtta tttctaacag cacccccaat
     2821 acacaggact atatagcggc ggcagattac gttattacaa aggctggatg gggaactgtt
     2881 gcggagtgta ttctcgcaca gcgtccgatg gcactgttcg cccgcgatac agttttggaa
     2941 gatagaacaa ctatttgtaa attgcaaaaa ctgggtctgg caatttccgt cacaccggaa
     3001 aacctccacc gcgtagagcg ggtgattgag gacctcaaac agctagacac ctcaaatttt
     3061 gatacatttt atgatgctgc gagcgaaatc gcagacaaat tgatttctct catgcaatga
     3121 gagatgctct aaatttagaa aggagcaagc attattatga tggaaaaaga cgcaaaaatc
     3181 tacgtagccg gtcatcgtgg catggctggt tccgcaatcg tgcgcgagct gaaccgtcag
     3241 gactacaaca atattatcac ccgcacccat aaggagcttg acctgtgccg tcaggacgct
     3301 gttgaagcat ttttcgcaca ggaaaagccg gattatgtat ttctcgctgc tgcaaaggtt
     3361 ggcggtatta tcgcaaacca gaacgctctg gcagacttca tgtatgaaaa catgattctg
     3421 gaaatgaacg tcatcaactc cgcatgggag aatggctgta agaagttaca gttcctcggt
     3481 tcttcctgta tctatccgcg tatggcaccg cagccgatgc cggaaagctg tctgctgaca
     3541 agtgagctgg aaaagaccaa cgaggcttat gcactggcaa agatttctgg cctgaagtac
     3601 tgtgagtttc taaacaagca gtacggtact gactatatct ctgttatgcc tactaacctg
     3661 tatggaccta acgacaacta tcatcctact cacagtcatg tgctccccgc tttgattcgt
     3721 cgcttccatg aggctaagga agcaggtctg cctactgtta cctgctgggg tgatggttct
     3781 cctctgcgcg aattcctata tgttgacgat ctggcaaacc tgtgtgtgtt cctgatgaat
     3841 aactatagcg gtgacgagac tgttaatgca ggtactggca aggaactgtc tatcaaggaa
     3901 ctgaccgaga tggtagcaaa ggttattggt tacgaaggcg agattctgtg ggataccagc
     3961 aagccgaacg gtacacctcg taagctgctg gatgtttcca aggcaaccaa gctgggatgg
     4021 acatataaga ctgagttgga agatggcatc cgtctgtcct acgaggactt cctgaataac
     4081 ccgatgcgtg ctgaacgcta atttaccgag gttgctgtga tttttgagga ttgcagcagc
     4141 ctctttctta taaaaaggag agatcattat gaatattgtt ttactgtctg gcggttctgg
     4201 taagcgcctt tggccgctgt ctaatgacat ccgttccaag cagttcatta agattttcaa
     4261 aactgaagat ggatatgaat ccatggttca gcgtgtctac cgccagatta agaaggtaga
     4321 taacgacgca actgttacga ttgcaacctc taagacacag gtttctgcta tccataacca
     4381 acttggtgag gatgtgggtg tatctgtaga gccttgtcgc cgcgatacct tcccggcaat
     4441 cgcattggct accgcttatc tggtagatgt gatgggcatt gatccagaag agtccgtagt
     4501 tgtttgcccg gttgaccctt acgtagaaga tgactatttt gaggccctga aatctctgtc
     4561 cgatcaggct gacaaaggcg aagctaatct ggtgctcatg ggtattgaac cgacttatcc
     4621 ttccgagaag tacggctaca tcattcctga gagcgcagag cagactgctt tcgtaaatac
     4681 cttcaaggaa aaaccttctg aggctgtggc aaaggaatat atcgcacagg gtgcactgtg
     4741 gaacggtggt gtgtttgtct acaagctgaa gtatgtcatg gagcgtgcac atcagctgat
     4801 tgactttgta gattatcagg atctgttcaa caaatacgat actctgacta agatttcctt
     4861 cgactatgcc gttgttgagc atgaggacaa gattcaggta cagcgttttg ctggtatgtg
     4921 gaaggatctg gggacttgga atactctgac cgaaggtatg gaggaatcca ttatcggtaa
     4981 aggtgttatg aatgagaatt gcgatggcgt acacatcctg aacgaactgg atgttcctgt
     5041 gttggctatg ggtctgcatg atgtggtcat ctgtgcatct cgggaaggta ttctggtttc
     5101 cgataaggaa cagtccagct atattaagcc tttcgttgac aagttcgagc agcagatcat
     5161 gttcgcagag aagtcttggg gtagttataa ggttatggat gtagaagcag agagcatgac
     5221 cattaaggtt actctgaatg ctagtcactc catgaattat cacagccaca agaaccgtga
     5281 cgaggtgtgg gtggttctgg ccggtactgg taagaccatc gtggatggat atgagcaggt
     5341 tgttaaggct ggcgatgtga tcaccatgag tgctggctgt cggcatacca ttatggcaga
     5401 tactgagctg aagctgattg aagtacagct tggcaaggat atcaatgtta aagataaaca
     5461 gaaatttgag ttagagtatt aaaaaacgga ggagggctcg ttatacagaa accgttttta
     5521 ttgaagcctt ccgcaaagga ttatctgtgg gggggaaccg cctgaatgat gaatttgcaa
     5581 aaggaattga gatgtctcct cttgcggaaa cttggaatgc tctacgcacc cggatggacc
     5641 gagctttgtt gtgtctggtc agagtatcag gggatgacct tgacggaggt gttgcaagaa
     5701 cacccagagt ttctgggaac gcatccgcag accaaaggag aattgccgat cctgatcaaa
     5761 ttcattgatg cgaaaaagga tttgtctgtt caggtacatc ccacagatga atatgcaatt
     5821 gaaaaagaaa acgggcagct tggaaagaca gagatgtggt atgtactgga tgccagctta
     5881 atctatgggc tgcagaaaat aacagaagaa gctgtgatcc gtgaagcgat tgctcaggga
     5941 actgttgagc gttatcttca gaaggttccg gtcaaaaaga atgatgtgtt ctttattgag
     6001 gcgggaacga ttcatgcgat tggtgcgggt gcactaattg cagaaattca ggaaaattcc
     6061 aatctgactt atcgtctcta cgattatgac cgtgttgaca aaaatggtaa aaagagagaa
     6121 cttcatgtag acaaagcgct ggaagtagcg aacttgaaag ggttgtcaga accgaaacag
     6181 ccgcttcgtg ttctaaaata tcgtgctggt gtagctgcgg aattgctttg cagatgcaag
     6241 catttcgagg tctctcgtat gattgtcaac actgagagaa gacagacggt acactatcgt
     6301 gcggattcaa tcagcttccg tgtgttgctg tgtgtcaatg gctgtggtac cgttcgattt
     6361 gataatgaat cgttggacat ctataagggg gactgccttt ttgtgccggc cgattctact
     6421 gaaatcagca ttcacgggtc gattcagttt ctggatgtga gaggataata atatgaatta
     6481 taaagtagaa tatgagcgtt ggctgaatca tgccaatgca gatattatag aagaactgcg
     6541 ttcctattct gagaaggaca ttgaggactc tttctataaa aatttgtctt tcggtactgg
     6601 tggtctgcgt ggtaccatcg gtgcaggcac taatcgcatg aacgtacaca ccgtaggtaa
     6661 ggcaagccaa ggtctggcag actatctgaa taagaactat gaagccgctt ccgtggtggt
     6721 tggctatgat tcccgtatca aatctgatgt gtttgcaaag actgccgcag acatctttgc
     6781 tgcaaatggt attcaggtgc acttgtggcc agaactgctt cctgttccta cagtatcttt
     6841 tgcgactcgt tatctgaagg cagatgccgg tgttatgatt acggcatctc ataacccttc
     6901 taaatataac ggctacaagg tatacggtgc agacggctgt cagattacta ccgaggctgc
     6961 tactgcaatt ctggcagaaa tcgaaaagct ggacatcttt gctgatgtaa agatggatgg
     7021 cgatgcttct aagattgcat ccattcagtc ggaagttctg actgctttca tcgaagaagt
     7081 gaaggggcag tccgtcctgt ttggcgaaga agtggacaag aatgttgcta tcgtgtattc
     7141 tcctctgaat ggtaccggtc tggtgcctgt tacccgcacc ctggcagaga tgggatacag
     7201 caatgtgacc gttgtggaag aacagcgtct gccggacggt aatttcccaa cttgtcctta
     7261 tccgaaccct gaaattaagg aagctatgtc tctgggtatg gagtatgcca agaagtacaa
     7321 tgcggatctg ctgttggcca ctgaccctga ctgcgaccgt gttggtatcg cagttaagag
     7381 cgcacatggt gactatgttc tgttgtctgg taatgagact ggtctgctac ttctggacta
     7441 catctgttct cagagaatta agcatggaaa aatgcctgct gaccctgtga tggtcaagac
     7501 catcgtcacc atggatttgg gtgaacgtat cgcatccaac tatggtgttc gtaccatcga
     7561 tgtgctgacc ggctttaagt ttatcggtga gcagattggt atgttggaaa aggctggtaa
     7621 ggttgacagc tatatcttcg gctttgagga atcttacggc tatctgtccg gtagctatgt
     7681 ccgtgacaag gacggtgtta acgctgctta tctaatctgt gaaatgttct cttactacaa
     7741 gactcagggt atcagcctgc tggataagct gaacgagatt tatacaaagt atggctattg
     7801 tttgaacacc ctgcattcct tcgagtttga cggtgcggca ggttttacca agatgcagga
     7861 aattatggtt gaattccaca agggtctcga tataattggt ggtaagaagg ttattgaaac
     7921 tttagactac agtaagggcc tgaatggtct gccgaagtct gatgttttga agtttatgct
     7981 ggaagataac tgctctgttg tggttcgtcc ttctggtact gagccgaagc tgaagaccta
     8041 tatcagcgtc agcgcagaga acaaggaagc cgccgaagct atggaagcta ttatggctgc
     8101 tgaactggcg gaaatgttcg agtaagaaaa gaaaggagaa atcttgtaat ggaggaactg
     8161 ttttctaacg ctttgaaatc ggtgaagtca aatcgtgtgc tgtacacggc atcgttcttt
     8221 gcaagatcct ctcttttgta tttgcaggaa attggacagc tgactgctct gaaggcacat
     8281 acatctaggc gtacaggatt gtcatcttac ctttgtttcc tggtagaagt tggttcaggc
     8341 acattggagt ataaggggca gaaatatacg cttcaagtag gagatatggt gtttattgac
     8401 tgtttgaagc cgtatgtcca tgagtcatca gaagatgatt tgtggacttt gcggtggtgc
     8461 cattttaatg gcattggtat gacggaagtc tacaaaaaat accttgaacg aggtggtcaa
     8521 ccggtcttta aaacatcaaa aacagataag tatgctgact tgctgaggag ccttcatatg
     8581 gtggcagagg gacaggagta tgtccgggat atgaaaatca atgttctact cagtgaactg
     8641 ctgatgcatt tgatggaaga ttcttggaat cctgacaaag tgccgaattt ctctacaaaa
     8701 aaaatggaat tgcagaacat aaaacaatat ctggatgacc attacacgga gaagattacg
     8761 ctggatcagt tgagtgcaga gttttttatc aataaacagt acttagctaa gatttttcgt
     8821 gaaacttacg gtacaactgt tgtcggttat ctgaatcaga aacgaatcac acaggccaag
     8881 cacttgctac gctttacaaa tctaagtatg gaagagattg caacaaaaat tggcataaca
     8941 gatgcaaact atttttccag aacttttttc aaggtggaag gtgttcgtcc cagcgaattc
     9001 cgcaagatgt ggtaataaaa agccggcggt gttgagccgc cggtcagtac ctgtcgttaa
     9061 cccgcattca aagctttaat ctcagctttg atttcagcac gaacacgata gtaatcttca
     9121 gtttcgattt cggtatccag ccgagttgct gtcttttcgc agtcaggaca acgctctggc
     9181 tgttcgtcgg cagggaagct gtagtggcag gaatcacagg tgtaaatgtg atcgtcacgt
     9241 ataatcatca cctaccaatc cagtgcttag atttttcatg caattactat tatatcgaat
     9301 tggtgattcc acaaaaatcc gcttcttgat aaatgtacga ttcagagtga tatatagtac
     9361 tgtactgcca aagggaatac cacaacggca gaaatggatg attcacatgg aagaaattat
     9421 gcagatgatt ttagggaacg caaaattcca gctcggacag gtggtttcga cccctgctgt
     9481 attggaaact ttcaaccccc tgcagaccgc aaacggcatt cccgaagcct tgagcaacct
     9541 ttggtttaga caggaaatta gtattgcgga gatcgaggac gcagttctgg atgccaagaa
     9601 cggcaaggag ctagcaaaca gcctcaaccg catcaagctt ttccgcaagt tcaccctcga
     9661 ccgagagctg gacggcatgg tcagactgaa gggcacggac gtctggaaga acatcagcta
     9721 cctcaaggtc tacaccgcca cgccgcctaa gaaaccgacc cccccaaggt tccagtcgaa
     9781 cggctggagc cttttcttct ctcataaatc ggcataatgt atacaaatac agctccaaat
     9841 gattgtgtag tatatgcctg tggattgact tgatatagtc gagagactat ggcaatatac
     9901 acacaaccaa aggggaaaat cccaaaaaca aggaggaaac gctaatggca atgagcgaaa
     9961 aggcaaagaa atatttgaag gagatcaagg gtgcaaagac catcccggag ctgaaggatg
    10021 tggagatcgc aatcaagcga gacggtatcc ttgcttgggc agagttcacc aagttgaacg
    10081 aggcggtgga agagaagaag gtcgcccttc gcaagaaaaa gcaggagacc agccttcagg
    10141 agatcctttt ctgggcctac aaaaaggaat cagacaagct gctgaagatg atggacgagg
    10201 gagcagacaa ggatgccatc cagatgcagg tccagcgtta cgacagcatc ggccagatca
    10261 ttgcagaggc agacctcgaa gatgagtatg aggtttgaga atacagcctg aaccactaag
    10321 aaccaaatac cgaaagggac ttctgaaaca gacagaggtc cctttctttt gctcactttg
    10381 gcggtcaatt atgaaccaaa aaccagtcga atgattgtgt acattatggc tttcaaacga
    10441 cttgataata atcactttta gagtgatata tgtactacaa aaaaacaaaa gactggagaa
    10501 aacaacagat gagcgaatac agaatcattt atatcagcga cgaggacggc ggcgaggtgg
    10561 aaggatgtgg ctacacggct acgatggtta tgatgcggtc agaaacttct tggaatttgc
    10621 ctgcggcatc ttgaaaatcc tgcgggtgcg attcatcaga agagtggact gaaatacaag
    10681 ttagcagata gagaagagag tcaggtttcg acttggctct gttctctgtc tactggcaga
    10741 aatacattga agggaggtga cgactgtggc aaccagagga agaaaatcaa ccccgacagc
    10801 catgaagaag ctggagggaa atccgggcaa tcgcccactc aatgcaaaag agtcaacgcc
    10861 ggaaaagaag gcaccgacct gtccgaagtg gctggaacca gaggcaaaga aggaatggaa
    10921 gcgtctggca aagcagatgg aggccatcgg aatcttgaca gatgtggata tggctgcctt
    10981 tgccggttag tgccaagcct acgcccgctg gaaggaagcc gaggaattca ttacccagca
    11041 cggtatcatc gtgaaaaccc tatccggcta ttggcagcag gtaccacagg tgtttattgc
    11101 ccagacctat ctgaaaatta tgaaccgatt ttccgagcag ttcggtctga cgccttcgtc
    11161 cagaagccgt atcattgccg gtgatggcgg tggtgtcgga gcagaagatg cgatggaggc
    11221 tctgttggga ggtggtagct gatggcagaa accagaccaa agggctatcc caaattaaag
    11281 gaatacacac caagccgatt catgctttcg gaatgtcact acgacaaagc cagagcggat
    11341 cgggcggtga actttatcgg acagctccga cacaccaaag gcaaatgggc aggcaaccgc
    11401 ttctggctct tgccttggca ggaacagatc atctagtgta atctcgctta actgtattaa
    11461 cggacgtagc gactttatta agagtgttca tggtaggagc attagttgca acaatgattg
    11521 atattaaatt tttatggtac caacgattgg cgatgtgaca ttgaatatgc aaaactatat
    11581 cgatatgata ttatctaaaa tcttactcgc tgggatcgtc gaggtgttta ttggttgtta
    11641 ggaaagaaaa atatgaccca actcgagcga tttttatcgt attacttgtg tgtggcttta
    11701 tctgcattgt gtgtgatttc gaaataaggg agagccaaaa atgagtagaa aattgatatt
    11761 gattagtcac ggaaaatttt gtgaggaatt gaaaaaaagt acaaaaatga ttatgggacc
    11821 tcaagagaat atctacacgt tacctttatt accagaagat ggcactgaaa catacagaca
    11881 aaagctctta ctgtcataga ttcgttaaat gattttgtgg tactatgcga tcttttaggc
    11941 ggtacaccta ctaatatcat ttctaaactg ataatggaag gacaacagat taaactttat
    12001 gtcggattaa tcttcctatg gtcattgaat ttatcaactc gcaagtgatt ggttcagagc
    12061 ccgagtttgt tcgtgcagga tgtgaaagca ttgtatctgt caactaatta attagtggaa
    12121 tagtagaaga tgaagagtaa taaaaagaga gtgggacaat agtcagaaaa aaatctgatt
    12181 attgccccac tttttttatt gactttagtc agtttcgttg gcgtagcgta cgaaacaaaa
    12241 aaacgcgcgt ttctacgcgt ggacgaaata gctttagcga taaaagttta gcacatacga
    12301 gatgggattg taagtatcac atagtcttta ccccgaagta tcgacgaaaa gtgatttatg
    12361 gaaagttaag agtaagtatt ggccagatat taagaaaatt gtgtgagagg aaagatgttg
    12421 aaatcattga agctcatgca atgcctgatc acattcatat gttagtaaga ataccgccaa
    12481 agattagtat ttctagtttt atgggctact taaaaggacg aagtgcaacc ttaatccatg
    12541 aaaaacatgc aaatttgaaa tataagtatg ggaataaaag cttttggacc aaaggatact
    12601 acgtgagtac ggttgggtta aatcaaaaaa caattgaaat atatatttga gaacaagaag
    12661 cagatgatcg aattagagac agtataagta aacaagagta tgtagatcca tttaaatagt
    12721 taaagagaga tagggctgtg agtggtgagc ccttctaggg ctgttaaaaa gccacccgtt
    12781 ttcacggatg gatatttact gattttaagg tacacaaaaa gcactcatcc ctgtaggatt
    12841 aaagtgacca aacccaatca gaaaggaagc gagtgcttta tgtttaaaaa ttataccatc
    12901 aaccaaaaaa ttttaccatt agatatggaa caatatattt ctgaaacaga tgtcgctttt
    12961 gcggtcaacg ctttagtcga agaaaagata aaagtggatt tatacgtgtc ttcaaaattt
    13021 acgaatgtga aagtggtcgg tgctgacctg ttcgtagtca atgtacgaaa gcaaaaagtg
    13081 agcgaaaccg acaaatttta gtgaataata catggcgtta tttcaaagca gaatgtaaaa
    13141 aaacttttag aggaaaaaga aggttcgatc tatcggaaac ggatttccac ttatgaagga
    13201 aacaaggagc caagattgac attggcttgg cactcatggc gttaaattaa gaaaattagg
    13261 aaaatacatg gagaggaaaa gacgtaaaga agaaaaaact attgtcccac tctctaagac
    13321 tgtagacaaa agcatcagtt tcctctttac taagagtgta gactgatact tttgtctatt
    13381 tttgtataat aaaagatata acgacatatg ctcccactgc caagtagcta attttttcag
    13441 attcattgcg acaaaaacaa gcattgtatg cacctgattt tttccaagcc tcgatacttg
    13501 gtgtagcgca ttccatgttt ttcctttgcg tctgcaaaaa tgcgttcaat cgtttctttt
    13561 ctttttttat atttttcacg attgtacacc gtatggcgta aatgttctgc ttcatctaag
    13621 tagtcttccc aaatatgtcg atgtatttct ttttggtgtt tttggctttc tgtacaattt
    13681 ttcaataatg gacaactttc acaaataaaa gatttagata catatttttt atagccatct
    13741 cgagtagttg tgcgaaactg atataaggga gaatgggtcg tagttttcgt tcgattaaat
    13801 acttgacgtt ccaaggtgtt ttgtacgcag cattagcagc tacaccataa cgtgatttaa
    13861 cgcgttcgat ttgtggcacg agcattcggc tatcatgtag atttccaggc gtaatcatga
    13921 catctaatac aaaaccattt tcatcaccaa cggtatgagc agaataagca aactgttttt
    13981 ctcgttctgt ttttacataa taaccacttt ctggatctgc gatgctaaat tttctttcga
    14041 ccacctcctg acgaatgatt gaaccaaaag gacgtttgcc ttcggcaatt cttgcctcat
    14101 tgatttcttc ttctaattgt ttttggtata aaatggcttc tttatgtgcc atatctcttg
    14161 taaaaatacg tttgttcgca ttcgctttaa tatgagttga atccatatac aaaatttctt
    14221 catgaataaa tctagcatca actgcttatt cgagaataat gataaatatc tgttcaaaca
    14281 aatcggtttc tctgaaacgc cgtgcatagt ttttaccaaa agttgaaaaa tgaggaactt
    14341 tatcaattaa actaagccca agaaaccaac gataagctag gtttgtttca acttatttaa
    14401 tggtctgacg catcgaacaa attccaaata aatattgaat gaatactaat tttactaaga
    14461 cgacaggatc aacgctaggg cgaccaatgg tcgagtaagt tgattcaaca atgagataaa
    14521 taaaatcaaa tgacaataat ttgtcaattt tacggactaa atgattttta ggaacaagtg
    14581 catctagcga aattttgtct tcttccattc gactatacat tgattatttg gacatcatta
    14641 tacattcctc ctcgaaatgc gaacgttttc tttaattgta acaaaaaaac tgtagaacaa
    14701 agaaattttt ttatttgtct acagtttttt gtcatatatt tttatttccg cttctgctgc
    14761 cttcctgcgt tgcggccttt gttgtttgta ctgttttttg gttgtttgat ttgttttgtt
    14821 gcttgtttgt tgtcagctgg ctttgctgat tctgtcacgt cttttcttgg agtaacgact
    14881 tgttttggcg ggttttgttt caactcttct gccacttgtg ctttcaaacg cggtttcatt
    14941 ataacgtttg tgatgaatgt ttgcagacag ctgaacacac caccgactac ccagtaaagt
    15001 gtaacaccgg ctggcgcact gatcgacatg aagacgatca tagcaggaga gacgatcagc
    15061 atcgtacgca tcgtcttttt ctgttcttca ggaataccga ttgttgaaat atatccttga
    15121 agcagataag cgccccctgc gatggccaca agcaccatac ttggtgagcc tagatcgatg
    15181 ccgtaaaaag atgattcacg gataccttct gtgtagcgag ccgtaaaata caaggcagag
    15241 aagatcggca tttggatcaa tagaggcaga cagccgattc cgccggttat actcaatcca
    15301 ttttcgcgat atacttgctg catttctgct tgtgctgcca tttgttcttc acgtgttgtt
    15361 gcagctttta gcttttgttg tgctgcttca acttgtggtt ttaacgcttg cattttttca
    15421 gattggatca ttgttttttt cgattgactg atacctagtg gcaagatgat gatacgaacg
    15481 atcacagtca ttgcaataac tgcccagccg taagaccaat tgaaattctc aactagatat
    15541 gtaatcgctt ggcccatagg atggacgagt actcgataaa cgattcctga agtgtcaggg
    15601 tttccgtttg aatccatccg cacacagcca gataaaaaga gcaggatact gacaagtccg
    15661 gatcccaata accagttttt ccatttattc atagaatact tcctcttcta taatttttac
    15721 ataaacttaa ctatactaaa aaagcgcatg g
//
LOCUS       DANKHK010000058        15627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000058 DANKHK010000000
VERSION     DANKHK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(101..1606)
                     /locus_tag="PW109_001730"
     CDS             complement(101..1606)
                     /locus_tag="PW109_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2511548.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1603..2289)
                     /locus_tag="PW109_001731"
     CDS             complement(1603..2289)
                     /locus_tag="PW109_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511549.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2482..3903)
                     /gene="gndA"
                     /locus_tag="PW109_001732"
     CDS             complement(2482..3903)
                     /gene="gndA"
                     /locus_tag="PW109_001732"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2511550.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4073..4252)
                     /gene="rpmF"
                     /locus_tag="PW109_001733"
     CDS             complement(4073..4252)
                     /gene="rpmF"
                     /locus_tag="PW109_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2511551.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4310..4879)
                     /locus_tag="PW109_001734"
     CDS             complement(4310..4879)
                     /locus_tag="PW109_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2511552.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5065..5967
                     /locus_tag="PW109_001735"
     CDS             5065..5967
                     /locus_tag="PW109_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2511553.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6002..7786)
                     /gene="pyk"
                     /locus_tag="PW109_001736"
     CDS             complement(6002..7786)
                     /gene="pyk"
                     /locus_tag="PW109_001736"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2511554.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7860..8822)
                     /gene="pfkA"
                     /locus_tag="PW109_001737"
     CDS             complement(7860..8822)
                     /gene="pfkA"
                     /locus_tag="PW109_001737"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2511555.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8990..12304)
                     /gene="dnaE"
                     /locus_tag="PW109_001738"
     CDS             complement(8990..12304)
                     /gene="dnaE"
                     /locus_tag="PW109_001738"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2511556.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12535..12720
                     /locus_tag="PW109_001739"
     CDS             12535..12720
                     /locus_tag="PW109_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2511557.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12782..13978)
                     /locus_tag="PW109_001740"
     CDS             complement(12782..13978)
                     /locus_tag="PW109_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2511558.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14148..15584)
                     /locus_tag="PW109_001741"
     CDS             complement(14148..15584)
                     /locus_tag="PW109_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2511559.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         4525 a         3333 c         2732 g         5037 t
ORIGIN      
        1 cgctagtttt tttataatga catccctgaa tttctcactt gcttgtattt tgtaccttat
       61 tctttgttgg atatataaaa aaacaagtaa agcacatgct ttacttgtct tctgcctttg
      121 cgataggaat aaagattcta aaaattgtcc cttgccctac tacactttct gctaagatac
      181 gtcctttata gttttcaacc agttgtttag cgatagacaa ccccagacca tttccaccct
      241 tcgttctagc tcttgcttta tccactcgat aaaaacgatt gaagatctta ttaaggtctt
      301 ctttgggtat tccttcgcca aagtcctgaa tcgcgatctc aaattcattc agagtggaag
      361 aaatagagat atggacttct tttcgtgtag ttgaatactt tactgcgtta tccaatatga
      421 taatgattaa ttgttcaaag tgattacgat aaatttgcag ggtcacttct tttgtcaaat
      481 catcatctaa agtgatcaca tagtctggat ataatatctt gaaattatta taaacttgat
      541 aggtgacttc ttttccttta gttgtttcat tcgcataatg aacatctact tgttctgctc
      601 ttgataagtc tagcatctct tgaactaagc ttttcatacg gctgatttct tgcatgctgg
      661 cgcttaatga ttcttctaat atctcaggat catctttccc ccaacgattt agcagactaa
      721 gatgcccttc aataattgca acaggtgtcc ttaactcatg agatacgtct tccacgaatt
      781 gctcttgttg ttcaatatac aaacgcatac ggtcaagcat ctcattgaat atttcagcta
      841 aatctgccaa ttcatcattt gtgtcgattt caggcatgtg gatatctgat tgcggatctt
      901 tacgtatagt atccattgta tctctgagta ccttgagtgg ttttaagaaa taagaagata
      961 aaaagaaacc taaaatacta cttaagatca aagaaacaac ttctaaaaca ataagagtca
     1021 ataatagctt atttcgaata tcatagaaag aagataattc atagaagctt tgcgcatagc
     1081 cgatcttttc tcttgtacct ttagaatata taggctggat agataaaaag ccagtttttc
     1141 cttctagtgt aacgatcgtt ggaactttac gtgtagtctg aatcaggttt cgactgtttt
     1201 catgggtttt aaaaatcaag ttctcgtcca aatcataaac ataaagatcc attgatggct
     1261 gccctagctc agctataaag gaatcgatct tcattaagtt tccttcgacc agtgaagtat
     1321 gatctacgac ctcatctccg tcatttgtcg ctttcgtcag attacgatac gtatttacta
     1381 atgtcagttc ttcatccgag ttagccaaac gagaagctac ttctgaaatc gtacgctcta
     1441 cattgtttct ttcttttgcc acaatcagat tgatagatga tttataggta atgacagcga
     1501 aaattgtaaa cacaacgaat atgaagaaag aactcgcaaa ggcccacttg attgtcaagg
     1561 agggcccttt cagttctctt ttttccgatt ttttcggctt tatcacgagc gcatcacata
     1621 acctgttccg cgtacagttt ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg
     1681 aagataacga atatatacgt ccactacgtt tgtttcaact tctgtttcgt atccccacac
     1741 tttatttagt aaaacgtcac gtgctaatac cacgttcacg ttttccatta aagtaagcag
     1801 caactcgtat tctctttttg ttagttcaat tacttcattt ccgcgacgta cgacacggtt
     1861 ttctttttcg atagtcaaat cgcgataagt aatagtagtt tgtttagcca catttttatc
     1921 gccttcaata tcgattcgac gcaacaatgc acgaagacgt gctaataatt cttcgatagc
     1981 aaatggtttg acgatgtagt catctgctcc gtggtcaagt ccagatacac gatcgatcac
     2041 agaatctctt gcagtcatca taatgattgg tgtatttttg acttggcgta cacgtctgca
     2101 gacttctaaa ccgttcaatt ctgggagcat cagatccaac aatatggcat cccactcatt
     2161 atttaatgcc gcatctaaac cagttcggcc gttgtagtgg acttctgcgt tatacccttc
     2221 atgcttcaac tctagctcga caaatcgtgc caagttcttt tcatcttcaa tgattagaat
     2281 attgctcatt tattatttta tcctttctat tctactggct ttttaaaaac tctatcatta
     2341 taccgataat atgcttaaaa atctatagtc attttatcat ggatagcaat tttaaaaaag
     2401 agctgaagtc tactcataac aatcgacttc agctctttcg tcctcttttt attttctcat
     2461 ttcctaacag ccgaaagact attattcttc atggtaccat gagtaatgga agattccttc
     2521 tttgtcgaca cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt
     2581 tgcaggcaga cgttctgctc tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg
     2641 tacaggtacg ccagcttgga cagccaaggc aacgacgtct ctgactgatt gctgatattt
     2701 tttcgtgatt tcaacgaagt aatcatctag caataaattt tcgatctcag gatttttgtc
     2761 ataggcatcc gtaatttttt gcaagaatcg agctcggatg atacatccag cgcgccagat
     2821 ttttgcgatt tcaccaaatg gcaagtccca atcaaattct tttgaagcga cacgtaattg
     2881 agcaaatcct tgggcataac tcatgatttt actgaaatac aacgcttctc ggattttttc
     2941 gaccaattct tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct
     3001 tgcttgtacg cgttcttctt tataagcaga aatatagcgg gcaaatacag attcagtgat
     3061 caatggaagt ggcacaccta agtcaagcgc gctttggctt gtccatttac ctgttccttt
     3121 attcccagct gcatcaagga tcacatctac tactggttta cctgttcctt catcgtcttt
     3181 acgtgtcaga atgtcagcag tgatttctat caaatagctg tctaattcgc cttgattcca
     3241 ttctttaaag atatcagcca tttcttctac tgaaaggttt aaaatattct tcattaagtc
     3301 ataagattca gcaatcagtt gcatatctcc atactcaata ccgttgtgta ccattttcac
     3361 ataatgtccg gctccatttg ggccaatata tgtgacacat ggtgctccgt cttctgcttt
     3421 tgcagaaatt tgctctaaga ttggtgcaac tagcgcatag gcttcttttt gtccaccagg
     3481 catgattgaa ggtcctttta aagctccttc ttcaccaccg gaaacgcctg taccgataaa
     3541 gttgatgcca gagttggcta actcttcatt acgacgcatc gtatctttaa agaatgtatt
     3601 tcctccgtca atcaaaatat cgcctttatc aaggtgagga agtaactctt ggattgttgc
     3661 atctgttgcc ggtcctgctt tgaccattag caagatccga cgtggtttct cgatagattc
     3721 tacaaattct tcgattgtat atgttgcttt gaaattttta tctgggtgtt cttctactac
     3781 agcggttgtt ttagcccctg tacgattgaa taaagccact gaatagccgc ggctttctat
     3841 gttcaaggct agatttttac ccataacggc cataccgaca acgccgattt gttgttttga
     3901 catatgaact cctcctacat acgggatttc tccctaatct cttacattat agcttaacac
     3961 ggctacattg caaactaaag aacaacatat tttttattta ccgactacaa aaaaataaac
     4021 ctcaaggctc catggttagc cttgaggttt tacattttct aattaaaatg tattaagctt
     4081 cttttgacat tacgtcctta ccatcatagt aaccacaatt tgcacatacg tgatggcttt
     4141 ttttcatttc accacagttt ggacattcgt ttaacccttt gattgtcaat ttgtagtgtg
     4201 tacgacgttt tgctttttta gcttttgatg ttcttctagc tggtactgcc attttgctac
     4261 acctccttgt aaagtcttgt atctaaatta gacacaatat attccaatct tacttgttat
     4321 cgtcgtcttt tgatttagaa tcaaaaagtt cagatagttt tgcaagacga ggatcaattt
     4381 tattctcggc ttcttccatt tttctgcgct ggtattcttc ttcggaaaca acttcccaat
     4441 cattgccttt tggaagttct gttgctgctt cctcttcttt tgtcaatacc cgcattggaa
     4501 tcgccaataa gatgttgtct tcaatcgact ctgctaaatt caataatgga ccttcaatta
     4561 gcaaaatctc atctgcagac atttgttcat ctctactttg gaattgttct tctgtcatga
     4621 atacttcgtc tacagaaaaa tccatgggca gttcaacagg ttctagtgaa cgtgttgaag
     4681 gaactgtcaa agtcgtctgc acagtgtaat gaacgatata gcctttctta tcaactgaga
     4741 ccaaaccttc gacttccact ggcgcaacat caagtattaa attgtctcgc ttcatgagag
     4801 cggctttcaa gtcgagcgtc tcatgaaaag ttaacggtgt ctcctggtat tttctcaatt
     4861 ccaataaaga ccatttcatt tttcatcact cctaagcaac aaaagcaatt atacagggca
     4921 aaaagcatgt tgtcaatgaa attttcttga caccttgcag ttcataaaaa aacagaaaaa
     4981 tctttattag aaagcctctg tttgctatca tcctcagaat catttacaat aatcattatt
     5041 ccattaaagg agcggatgat cttcatgaac acgaaagata tcgaatacta tgtccaaatc
     5101 gtcaaacaaa aaaattttac gaaagtggct aattatttcc gtgtgagcca acctactatt
     5161 acatatgcat tacaacgtct tgaaaaagag tttgacaccg tacttattac aagagatcga
     5221 tcgcatcacg aattgatcgt cacgccttct ggaaagcagc tgtttctcca tgcacaaacc
     5281 attctccaag agatttctac tgcaagagct gaaatcactc agttgcaaaa gaaaaagctt
     5341 cgttttggca tgcctcctat tatcggcaat gaatactttc ctaaactgtc aagttacttt
     5401 atcaagcatg aattgatgaa tcaaattgag attaccgatg gaggctctcg ggacttatac
     5461 gggatgatcc gacaagggag gatcgattta gctgtattag gctcaacaca gccaatcgtg
     5521 gatcaatatt tagaaacaga gttacttttg gacaaacgtt tcatgatcgt cgtgccacct
     5581 acacatcctc tttccaagca ggatactgtc tctttcaaag atatagcaga tgaacagttt
     5641 gtactattga acgaacatta tgtccacccc gcagcattca ctaagctttc taaacaaaca
     5701 catactcatc caaaagttgt ttatcaaagc aatgacttga cgattttaaa gagtatggtc
     5761 cgagaaaatg tgggtatcgg ttttttggca gagattgctg tccatcctga agatcattta
     5821 gtttgcctcc ctctttcaga tgaagttcag cctcgcttct tgatttcatt ggcaaaacga
     5881 acacagcaga tcgtcacgcc tcttcaagaa caagtagtcc aagttatcca tgaattcgtt
     5941 acagcacaaa aaaacagctc ttcgtaaaag aagagctgtt tttatttttt attaagctaa
     6001 attagatagc tgttgtttcg ccacggtaaa cgatgccgcg acgtgaatca acggtaatca
     6061 attcaccttc tgtaattgtt gttgttgcgc ctttagcacc aacgattaca ggaatatctt
     6121 gtgcaattcc tacaactgca gcgtgagaag tcaatccgcc ttcttcaacg ataacagcag
     6181 ctgctttttc gattgcaggc atatattctt tgtcagttga tggtacaact agaaccattc
     6241 cgtcttttgc tttttttaca gcttcttcag cagatgaagc aataactgca tttgcaacga
     6301 cagatttttg tccaacgcct tgagcttcta ataatttaga accaatcatt tgaattttca
     6361 tcacgttagt tgttccgcgt tcaccaactg gtacaccagc agtgatcaag attaaatcgc
     6421 cttctgatgc gaaacctaat tcaacagctt ttttagctgc tagttcaaac atttcatcag
     6481 ttgttgatgg tttttcagca actgttgggt aaacgcccca gtttaacatc aaaccacgtt
     6541 ttgtgcgttc gtcaaaagta acagccaaga tatctgcatc tggacgatat tttgaaatca
     6601 tttttgcagt gtatcctgat tcagttgcag caacgattgt tttcacgcct agattttttg
     6661 cagcacgagc tacagataaa ccgattgttt cagttacatc tgttttatcg aattcgttca
     6721 attggaatgt acctaattct gataatgcat tttcagcttc ttggtcgata cgtgccattg
     6781 ttgaaacagc ttcaactggg tagtccccgt ttgcagattc accagatagc attgttgcat
     6841 ctgtgccatc aaatacagcg ttagcaacgt cagaagcttc cgcacgtgtt ggacgtgggt
     6901 tttcttgcat tgattctaac atttgtgtag cagtgataac tgggatacca gcagcattac
     6961 attttttgat aatgcgtttt tgaaccattg gtactagttc agctgggatt tcaacaccca
     7021 tatctccgcg ggcaatcata acaccgtcag aaactttgat gatttcgtca atgttgtcga
     7081 taccttcttg agattcgatt ttagggaaga tttgaacatg agtcatgtct ttttcttcta
     7141 agatttcgcg gatatctaga acgtcttgtg ctttacgtac aaaacttgca gcgataaagt
     7201 cgatatcgtt gtctaaacca aaacgaatat cgtcagcatc ttttggagta atacctggca
     7261 aactaataga aacgcctggt gcatttaccc ctttacgtga tcctagcata cctgcatttt
     7321 taacttctac gactaattca cggtttgctt cgtctttttc aatgatttgc atatcgatca
     7381 aaccatcatc aaataaaatg tgtccgccaa cgtgaacatc gtcaaataat cctgggtaag
     7441 taaccgcgat tttttctttt gaacctacat gttcaggatc catagagatg cgtgtttgat
     7501 ctcctacttc aaatttgatg tatcctgcac gaccaaaatc agcttctgtt gtgccttgaa
     7561 cagttgtacg gatttcagct ccttttgtat ctaaaagaat accgacatct tttcctgtct
     7621 ttttaactgc ttcacggacc atagtcatac gtgataattg ttcttcatgg tcaccatgag
     7681 aaaaattaaa gcggaagaca tttgccccag attcaatcaa ttgagaaata atttctactg
     7741 agttacttgc tggtcctaat gtactaacga ttttggtttt tttcatttaa aaaactctcc
     7801 cattcaattt tgtttattta aagctgtaca cagcttaaat agcttggttt ctttcactct
     7861 taaaatgaaa gtgaattatt tagatcatat agtgataagt ctggtttatg tttgttgttt
     7921 tcaagtgtat caatgatgtc agacgcaacg atttcgttgt catggatacc gacacataat
     7981 ccaccttttc cttcttgaag caattcgaca gcgtaagcac cgaatttact tgccaacacg
     8041 cggtcgcgtg cgcttggtgc tccaccacgg acaacgtgtc ccaagataga tacacgtgta
     8101 tgatagtcac caaattctga aagtttctca gcgaattcat ttccgcccat tacaccttca
     8161 gccaagatga tcaagcaatg tttcttcccg cgatcgcggc cttcttggat ttttttagca
     8221 actgaagcca tatcaaaatc atgttctggg atgatgattt catccgctcc gccagccaca
     8281 cctgaccata aagcgatgtc tcctgcattg cgtcccatta cttcgataac gaatgtacga
     8341 acatgagaag tcgctgtgtc acgaatacgg tcaattgatt ctagtactgt attgattgct
     8401 gtatcaaaac cgattgtgaa atcagtccca ggaatatcat tatcgattgt tccaggaata
     8461 cctactgctg ggaaaccgcg ttttgtcaaa gccattgcgc catgataaga gccgtctcct
     8521 ccgataacca ctagaccttc gattccgaat tttttcaatt gttcgattcc ttttaattga
     8581 ccttcttcag ttgcaaattc tggataacgt gcagaataca agaatgtacc accacgttgg
     8641 atcttgtcac ctacatctgc cacatcaaga cgacgaatat ctccagcaac taaaccggca
     8701 aaaccataat tgatcccgta tacttccagt ccttcgtaaa tacctttacg aacaacggca
     8761 cgaacagctg cattcattcc aggtgcgtct ccgccactgg tcaaaattcc gatgcgtttc
     8821 ataataaaca ttcacctcat taaaatactt tatcctaagt atgacttagg tttacaacca
     8881 tttttgcgtt aaccacgact aacattctaa cattattttt ctcacttgtc ttgatttacg
     8941 gataaaaaaa tgaaaaaaaa tgaaagcaag tagtattttt taccgtaagt tatttgaaaa
     9001 ccacattatc ttcgccaagc agataaataa gttgccgctt tatactcttg ctgtcatcca
     9061 cccaataagc atctgacaga actacttttc gttgcgtttt ttcgtgatac ataactactg
     9121 gtatatatcc tgggtgtttc tgaaaaatca cttgtaattg tttcattatc tcgggatctt
     9181 cccgttcttc tgtaatacgc aaatagcaag tttgtgaagg atactgggct tctaactggt
     9241 taggatcagc aaagctttct ccaattagtt gcagttcctg attatatttg ctgatttcgc
     9301 tccgcccttg aacaaagatc actcgatcaa cggcaaacaa attacgatat ttacgataaa
     9361 gagttggaaa aactgtgaca gaaatttctc cactactgtc ggttccttca acaaaggcca
     9421 tcagttctcc tttttttgtc cgaatttctc taacttcctt gatcatcact aataaattga
     9481 ctggttgatt agggacgata tctgtaatca gctgaacttg tttagctaaa cgaagacgat
     9541 caaacccttc tgcaggatga cctgacaaat aaatacctaa gtactcctct tccagcttca
     9601 agcgttcttc tagtgtatag tcagcgactt cttcttcttt taatgccatg attcccagca
     9661 agtcaagact tccaccactg taaacgatat tttggatttt tccttcgaga tcaagcatca
     9721 attgtttcct gttaggagat aattcatcaa aaactcctac tgctgtcagt gattgcagta
     9781 actctaattt cagccaacgc ttatcgatcc gtatgaggaa ttgatccact gactggaacg
     9841 ggccattttg ttttctttcg tttaatatat cttctacaaa atcacgccga atacctttta
     9901 tcgctgtcag tccgaatctg atttcttcct tattgaccaa ctcaaaactg gaattacttt
     9961 gattaatgga cggcgaggaa agtttgagtt tatttttacg tgcttctgca atatattctt
    10021 tcaattttga tgtgttatgc cgaacagaat tcatcaggct ggtaaagaaa gcacctggat
    10081 gatgggcttt tagatacgcc atttggaatc ccacgaatga ataagcaaat gcatgtgacc
    10141 gattgaatcc atagtttgca aatttttcga tatattcata tacggtcgct gctgtttttt
    10201 ccgaatatcc ctgttgtaat gctcctgata caaaatgcga cttttcttca tctaatacgt
    10261 cttttttctt tttactcact gcccgtcgca gaatatctga ctgccctaat gtaaatccag
    10321 ccatagtcga ggcgatctgc atgatttgtt cctgatagac gatcactcca tatgtgtttt
    10381 tcaaaatcgg gatcagtgaa tcatctgggt agcgaatggc ttcttttcct ttcttcctgg
    10441 caatgaaggt atctatattt tgcatagggc ctggtcgata caatgcattg actgcggcaa
    10501 tatcttcgat gttttctgga tctaatcttc gtaaaacgtt ccggatacca gcagattcaa
    10561 attgaaatac tcctgaggtt tcgcctcgtt gaaacaagag cagcgttttt gcatcattta
    10621 gcgggatctc ttttaatcgg atctcatacc cttctactcg cttaacagct ttcaaagtaa
    10681 aatcaataat cgataagttg cgtagtccta aaaagtccat tttcaaaaga ccgattgctt
    10741 ctacctcatt catcgtatac tgggtcagaa aagcttcatc agaaccgcgt tggagcggaa
    10801 ctagttcaag tagattctta tcgctgataa ctacacctgc tgcgtgagtc gaaacatgtc
    10861 ttggcaatcc ttctaaaatc actgcagttt tgaataaaag ttggtttttt tcgcttgcct
    10921 gaactaattc tactaattga tgcgaagttt tataagcttg ggtcaatgtg attttcaatt
    10981 gattgggtat tgcttttgac cagcggtttg cttcactttg cgataaacca aacactctag
    11041 ctgcatctct aagaaccatt tttgctgcca ttgtgccaaa tgttgcgatt tgtgccatat
    11101 gatattgtcc atacttttct cgaacataat tcaatacttc ttcacgtcga ttgtcaggaa
    11161 tatccaaatc gatatctggc atcgtaaagc gttcggggtt taaaaagcgt tcgaacagca
    11221 aatcatattc taatggatcc acatctgtga tttctaacac ataggctact aatgaacccg
    11281 cagcagaacc acgacctgca ccggtaacga ttttgtttct atgagcaaaa gccattacat
    11341 cccatacgat caagaaataa tcatcgaatc ccatttgatg gatgacagat aactcgtagt
    11401 ccaaacgttc ttggtattcc tttgtatagt cagtgattcg gcgttccact tgcgaatagc
    11461 ataactctct taaatattct ccagcttgtt ttccattagg aactggataa tgcggaagta
    11521 atttctgatg gaactggaat tcaaatgaaa tcgattcagc taaacgctgg gcgcctatta
    11581 cagcttggga aagacccgct ttttcgaatc gtttgatcat ttcttgttct gaaagaagat
    11641 agacatttgc tgttagtggt aaagatgcag ataaattttc taaaggtatt tggcttccct
    11701 ctttgatatg tttcatcact tcaagtgcaa aaagttcttc ttgttgtaaa ctagcgatgg
    11761 gatgtaatgc acaaagcgaa taattttctt ttatcacata ctgataccat tcaggcaatt
    11821 gatcaggcgt gttttctgtc aagccgatat aaaaatttct agtaaaaaca ttttcaagta
    11881 aaaactgcca atgaccgaaa aagttatcct ctttattgaa agcctcagaa acagaatgat
    11941 ttgccggaac aacaaacgct agatggtcgg tttctgtcat ttctgcaagt tccaattttt
    12001 cttggatcat ttttcggctg gaaagtttca tcagctgctg ataacctatt tcatcttttg
    12061 cgtacgcaag caagccataa tttttttctt cataagtata actaagatgt aatccaatga
    12121 ttggctgtac tccttcttct acacaaatac gatagaattc aggtgcaccg tataagacat
    12181 ctttatccgt tatggctaat gaagtgtagc cttgtttttt tgcttcttgt acatattctc
    12241 gaatacgtat cgtactttgt aaaagagaat aagaagtaat tgtataaagt tgtgggaaaa
    12301 acatcttgca ttccgccttt tttttcgtta aaattcacta gcgttaaaag gtttgatcct
    12361 tttactttca ttaagatagc aggcgctgaa gttgattatt ccacttcatt tgcttgcttg
    12421 taatctgtag gaatgagttc tttcgcaaga actcaaattt tattgagatt tggtctcgtt
    12481 tctttacatt caaagaaaat ctggtaaaat taagatagaa aagaggtgtt ctttatgcga
    12541 tatatcgtta cattattctg ggctgtcgtt ctaggtcaag tagtcggtta tattggtgct
    12601 gcattaacaa gcggaacata tgattttact ttgacaacaa tcatttcatt cattgcgggt
    12661 gtcattatcc ttttgattgg tgcagttgcg ccaagaaaag aaacctctgc ccattcgtaa
    12721 aaaaacagcc aagagcttga gatttctcaa gctctttttt attttctact cctcaccacc
    12781 attataatct gctgcgaaga actttacgaa agttgaataa agaaagaaga atatttgaca
    12841 gaacaaacaa acttgtcaca ataaaaacag cactgtaatt ataagcatgc gcgactgtcg
    12901 atcctagcat gggacctaaa acttgtccaa agttgctaaa catctgattg tagctataga
    12961 ttcggctgac tccttcttga ggcgtaatct tactgatcag tgtattgaca gaaggcatca
    13021 aagcgccagt tgaaaagcct aaaataaagc gaagtatacc taattggagc ggtgtagtga
    13081 cgaatgccat aggtaaataa cagcaaaatg ataaaatgag tcctccaatt agaacttttt
    13141 gattcccaat cttatcccct agttttccta gtttcggtga agaaaagaca gcggaaacac
    13201 cagctatcga aacaatcaag ccgctgacaa aaaggatatt ttctgtactt ccgctcaatt
    13261 cacgaatata taaggtcaaa atagggctga tcgtagtgat tcctatctgt aaaatcaagg
    13321 tcgtaatgaa gagaccgatc aaaatagaga gatgatccat ttttgaaaaa atttctttgg
    13381 tgcttatcag atcttttttc tcgattggat ggaaatcttc tttgactaag aaaatggtca
    13441 ataccgttgt gatcatcaac aaagcccctg tgatcaagaa tacattttcc ataccaaacc
    13501 actgagccag tgcaccgccc attgaaggtc cgatcagatt tccagcaatc gctccggtag
    13561 acaatgttcc taacgcccat ccgctctttt ctctaggtgc ctgtgaagca atcatcgctg
    13621 tggcatttgg aatatatcca gacaaaatcc cattccaaaa ccgcatgatc aaaagccaat
    13681 acgcgtttgg tacaaaagcc aaagatccca ttgttaatgt catccctgct gctgcacgga
    13741 tcatcatgag ttttcggcct tttcgatcag ctagatttcc ccagattggt gcaaccacgg
    13801 cagatgcaaa agcagtcaca gagattgcta gtcccgaaaa caattcgatt tgatcttttg
    13861 gtgttcctag ttgctcaaca taaactggta tgaatggcat aactaaacta atacttgcac
    13921 cagtgaaaaa acagccaatc cacgcaatca ctaaattttt tctccaatta atctccatct
    13981 atcatccctc tttcgtctct tttatcatac ctattttcga ataaacaagc aacgatctcg
    14041 ccagtaaaaa gagaaaaatc ccgcaaaggt cattttatat gtaacgttct ttttatgtaa
    14101 aacaaaaaga gatcagaaca aatactgttc caatcccttc tctttagtta aattgttgct
    14161 agaatgatga agttgataat gaataaaata tcaacgatcc acaaagcaac agaaacttga
    14221 ttgtattttc ctttgattac tttcacaatt gtatagaaaa taaatcctgc tgcaatccca
    14281 taggaaatgc tatagcacaa tcccataaag atcgatgcaa agaaggccgg taaagcttct
    14341 tccatatcta gccatttgat gtctgcaaaa gaagccatca tcattactcc aactaaaatc
    14401 aatgctggcg cagttgcttg atttggtaca atggcgatca gtggagagaa taaactactt
    14461 aatgcgaata gcactgccac tactactgaa gtcaaaccag tacgtccacc ggccccgatc
    14521 ccagctgcag attcgacata agttgttgta ttcgatgtac caaagatagc ccccacagaa
    14581 gtagcgatcg catcggcaaa tagtgcttta tccatttttg ttttgaaccc acgaccatct
    14641 tccaacgcga tctcgtcttc ttttgagaaa atacctgtgc ggcgtcctgt accgataaat
    14701 gttccgatcg tatcgaaagt atcagataaa ctgaatgcga tgatcgtcat caatacttga
    14761 ggaatcttgg aagaatcact gaataaagat tgcatgcctt cagaaccaaa tgctgcaccg
    14821 aaagttgttc ctaattcttt aaatgaatta cctaacgaat tcgtttgcca atcaatggaa
    14881 cttaaatcaa caacacccat aaaaatacca aggactgtgg tcgctacgat accgataaga
    14941 atagccccac ggacattttt tacaactaaa atggctgtca gtaccaaacc aatcacagcc
    15001 aataagatag gagcgttgtc aaagttggct aacgcaggga cgattccacc atttatcgta
    15061 acatttgtcg cttttccgcc ttcaacgaca gagctcgaaa tagctgactg gtctgctgaa
    15121 aaagataaga atcctgcatt tttgattccg acatacgcaa cgaaaatacc gatcccgcca
    15181 ccaatcgcat gttgaagact ttcgggaatc gcccgaataa tcatttttcg aatatttgtg
    15241 acagtaataa aaatattgat taatccgcaa ataaacacca ttgccaaggc ttgttgccaa
    15301 gaataaccaa gcccaaaaac gaccgtaaaa gtgaaaaatg cgttcaatcc cataccaggt
    15361 gcctgagcat atggtacatt tgcgaacaat cccattacca gcgtaccaat aatcgatgca
    15421 atgattgtcg ctagaaatac tgcttggaag ggcatacctg atgctgacaa aatcgtcgga
    15481 tttacgaata agatataact catggcaaag aatgtcgtta ctcccgccat tatttcagtt
    15541 gatacatttg tattgttttt ctttaaaccg aaaaactttt ccataaaaag ttccactccc
    15601 tgaatttttt tctgatggaa aactata
//
LOCUS       DANKHK010000059        15194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000059 DANKHK010000000
VERSION     DANKHK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(149..334)
                     /locus_tag="PW109_001742"
     CDS             complement(149..334)
                     /locus_tag="PW109_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511560.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1184..1525)
                     /locus_tag="PW109_001743"
     CDS             complement(1184..1525)
                     /locus_tag="PW109_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2511561.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1525..1755)
                     /locus_tag="PW109_001744"
     CDS             complement(1525..1755)
                     /locus_tag="PW109_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2511562.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2043..2381
                     /locus_tag="PW109_001745"
     CDS             2043..2381
                     /locus_tag="PW109_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511563.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2586..3161)
                     /locus_tag="PW109_001746"
     CDS             complement(2586..3161)
                     /locus_tag="PW109_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2511564.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINACAETVEEKKTFHQPFLQNRCVFPMSGF
                     YGWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3526..4107)
                     /locus_tag="PW109_001747"
     CDS             complement(3526..4107)
                     /locus_tag="PW109_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2511565.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4290..4916)
                     /locus_tag="PW109_001748"
     CDS             complement(4290..4916)
                     /locus_tag="PW109_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2511566.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4938..6266)
                     /locus_tag="PW109_001749"
     CDS             complement(4938..6266)
                     /locus_tag="PW109_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2511567.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTSSLRLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6424..6825)
                     /locus_tag="PW109_001750"
     CDS             complement(6424..6825)
                     /locus_tag="PW109_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2511568.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6911..7117)
                     /locus_tag="PW109_001751"
     CDS             complement(6911..7117)
                     /locus_tag="PW109_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511569.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7212..8714)
                     /locus_tag="PW109_001752"
     CDS             complement(7212..8714)
                     /locus_tag="PW109_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2511570.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8732..9187)
                     /gene="argR"
                     /locus_tag="PW109_001753"
     CDS             complement(8732..9187)
                     /gene="argR"
                     /locus_tag="PW109_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2511571.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9285..9692)
                     /locus_tag="PW109_001754"
     CDS             complement(9285..9692)
                     /locus_tag="PW109_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2511572.1"
                     /translation="MYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQLMIRQPDFVTEAI
                     AQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFEKLEAFIAKEGYE
                     RASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            complement(9707..9901)
                     /locus_tag="PW109_001755"
     CDS             complement(9707..9901)
                     /locus_tag="PW109_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511573.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKIG"
     gene            10122..11495
                     /locus_tag="PW109_001756"
     CDS             10122..11495
                     /locus_tag="PW109_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2511574.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11570..12682
                     /locus_tag="PW109_001757"
     CDS             11570..12682
                     /locus_tag="PW109_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2511575.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12754..14391)
                     /locus_tag="PW109_001758"
     CDS             complement(12754..14391)
                     /locus_tag="PW109_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2511576.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWAHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFITCHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSCYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14559..15167)
                     /locus_tag="PW109_001759"
     CDS             complement(14559..15167)
                     /locus_tag="PW109_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2511577.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4195 a         3113 c         2662 g         5224 t
ORIGIN      
        1 actattaatt tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc
       61 actttctgtc acatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga
      121 cgctggaata gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa
      181 aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca
      241 gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata
      301 agatggttag gaatccttta gaatttttac tcattcataa acacttcctt aaatagcttt
      361 tattatagaa ggtattgtaa ataataatat tgacaaaaag tgactcaaaa taaaaaaaac
      421 cttacattat actggaatga actgtattga tagtgtacct aaaagctcta ctgacataag
      481 atcgtgtaat cggtaaagta attttattaa ttatacggga tgtaatcaat tagagaataa
      541 actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc
      601 actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact
      661 aaatagaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag
      721 tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat
      781 tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg
      841 agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac
      901 taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat
      961 acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg
     1021 aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt
     1081 aatgccatcg accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt
     1141 tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta
     1201 taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc
     1261 gttccttaaa atcaagagat tttaattgtg aaataattac ttgtcctttt atatctagct
     1321 cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga
     1381 ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt
     1441 tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag
     1501 gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc
     1561 ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt
     1621 tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga
     1681 aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga
     1741 ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa cactaagtaa
     1801 cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg
     1861 gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca
     1921 agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac ccccccgcta
     1981 ccttcaattt tgcagataaa acgctttggg agaaaatcgc aaagacgaca ggccatccta
     2041 tcatgcccga ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg
     2101 gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc
     2161 ttcaaataga aacattggtt caacgaattt caactagaca agactttgct attgtagaac
     2221 gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg
     2281 tggataaatg gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt
     2341 taactttgaa ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta
     2401 taaataaaaa agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt
     2461 cgactgtgac cctatagatg cagtacatgc agcttatgag catttcttct gttctctttg
     2521 tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga
     2581 ttatgttata ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca
     2641 aaagaaatat ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg
     2701 tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt
     2761 ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa
     2821 aacataaatt tgtctttttc ttcattccac ccataaaagc cactcattgg gaaaacacaa
     2881 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacaggca
     2941 ttgatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     3001 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     3061 gctgctaatt ctgctgcctg ctccggttgt ttcttctggg cttctcgata ataattctga
     3121 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaacctt
     3181 tttttctatc atacactgca acaagaagtt cgtaccaaca aaacgctttc aatttgtgta
     3241 gaactaaaaa aattgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3301 cctcggtcac aagtcacaat attcaacaaa catgagaagc tattttttga aattgttcct
     3361 tgtgacccga agtgaaacgg caggcttacg acctctgggt aaacggtgtt ccagaagtag
     3421 cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaatgcc
     3481 ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg
     3541 cgaagttaac aatctttctt tgatcaattg gaaattctgt ctaagtagtg atagatcttg
     3601 tgtgactttc aacaataaga tactgccctg gatttcagca atcaacgctt tggcttgacg
     3661 gtctgcctct gcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata
     3721 cttgacccat tgattgacta attctagtag caacagacgg tattcttcat ccactgtact
     3781 caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc
     3841 agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa
     3901 gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca
     3961 aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc
     4021 tagaatatct cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc
     4081 ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta
     4141 tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat
     4201 tatctgacac agccctcatt ataaaactgt tctcatatag ctagatgttc attgattcaa
     4261 gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt
     4321 gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttga
     4381 ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc taccaattca
     4441 cgcatatggg aatcgctaca caagtttccg ttcattccac cgagaattac tgtgtcgatt
     4501 ccattttttc tcaattgaag cgctaagtcg ttgctttctg gtccaaagat tttatgagga
     4561 gagctgatga tcgtattttc atccaaatat ggtttcaatt cttcgatgaa atcagctcct
     4621 gaaccttctt caactgattc atattgactt ttcctagcaa acatctgctg ttcaagcatc
     4681 atgtgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga
     4741 gaaataaaga gtttgtatcc tttttctttt gcttgtttca ccatttcgat catgttttcg
     4801 atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc
     4861 aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg
     4921 gattctccat tcgttattta ttttaataca tcatttaaag cttcactcat tgttggatga
     4981 gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc
     5041 aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt
     5101 tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg
     5161 acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt
     5221 tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc
     5281 cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca
     5341 tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg
     5401 tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca
     5461 tctgtatttt ctaatctaag tgatgatgta cttggttttc ttccagttgc agccaaaact
     5521 tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat
     5581 tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc
     5641 atgtcttcta aaatcatttg cgcgatatct tcatcttcac gaggaagaaa ttcaggatgg
     5701 gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca
     5761 atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc
     5821 gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca
     5881 gaaccagtat tgatgaaaaa ccgttcgcct tcaacaatca ctttttcacc attattcgtc
     5941 acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc
     6001 tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga
     6061 gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga
     6121 atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa
     6181 accgattcgc cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg
     6241 atcacaatat tttgatattt ttccatcctt tttccctcgc ttctttttta cctaccgatt
     6301 ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca
     6361 aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt
     6421 cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca
     6481 gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg
     6541 cacttctaat actacaccga tctgatagcc accatcatct aaattataaa gagagacacg
     6601 tgcttttact gtggactctg ctgtcatctc tgcttcttta atcatgtgct gtactgcacc
     6661 attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg
     6721 gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt
     6781 gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc
     6841 gattgaccat ttgctacttg gctataacta tagtgtatga caagtaaggc gctttctaca
     6901 aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa
     6961 aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta
     7021 cataatcttt tatatgattg attttaagat acatatatac cagaaagttg accccataaa
     7081 ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc
     7141 cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca
     7201 aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt
     7261 gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact
     7321 agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct
     7381 agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc
     7441 aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac
     7501 aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct
     7561 aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat
     7621 gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct
     7681 agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag
     7741 ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa
     7801 gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt
     7861 aatactgcga tcaaattatt tcgattgatc actgttccgg aagcactgac aactgcagaa
     7921 cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc
     7981 ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca
     8041 tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca
     8101 gcaaagaatc caccagcacc atttccagca tccacgatga ttttccaacc ttttagtggt
     8161 ttgtcatctt gtgttcccat cccttttttg atttttgtga ctaaatcttg cgcataacgt
     8221 gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt
     8281 gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt
     8341 ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac
     8401 tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca
     8461 tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc
     8521 ctactgtcat gaccaatccc aatcgtccat tttttatttg gattggttga cttcttcttt
     8581 ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt
     8641 acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgtagcgct
     8701 ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg
     8761 caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca
     8821 tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc acatcggcac
     8881 catttcgttc agtcaatacg atgaccatga actgaacaga ctggatacgc aaaacctcgc
     8941 gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg
     9001 tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc
     9061 gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt
     9121 cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct
     9181 ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat
     9241 attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt
     9301 ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa
     9361 atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat
     9421 ttctcaaacg ttgctggttc ttcgtcatag ggcccgacgt gtaagatttg ggcacaaaga
     9481 ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa
     9541 cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat
     9601 tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc
     9661 cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt
     9721 taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt
     9781 cttcctgatt cgggtcgccg atccctctga tgctaaagaa tggtttttta ggaacctcta
     9841 gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca attttttcca
     9901 tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac
     9961 ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat
    10021 cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa
    10081 atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc
    10141 tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg
    10201 agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac
    10261 aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta
    10321 cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct
    10381 tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat
    10441 tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat
    10501 ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg
    10561 ctccagcaat tggtcccact tatggaggca ttatgacttc acattttaca tggaatcata
    10621 tcttcctgtt tcttgtgccc gttttgattc tttctttgat tatcggctgg tttgctattc
    10681 ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc
    10741 ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg
    10801 ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc
    10861 atccgctgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct
    10921 tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga
    10981 tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg
    11041 gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa
    11101 tttttgctgg actgattctt tctttgattg gttggctagc attattccta ctcataggac
    11161 acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat
    11221 cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg
    11281 ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag
    11341 cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat
    11401 taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat
    11461 tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat
    11521 ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat
    11581 tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa
    11641 attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat
    11701 tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg
    11761 cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca
    11821 atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga
    11881 gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac
    11941 atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg
    12001 tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga
    12061 gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc
    12121 gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt
    12181 attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt
    12241 tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga
    12301 tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg
    12361 ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt
    12421 cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc
    12481 ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc
    12541 tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc
    12601 caacttctta aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta
    12661 tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt
    12721 gtgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc
    12781 caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg
    12841 taaacgaata tcaagtcgtt gtttcaccaa atttttatag gacgtatcct caaaagaaac
    12901 aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc
    12961 tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt
    13021 atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc
    13081 tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc
    13141 aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt
    13201 tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg
    13261 aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac
    13321 tcggtcgggt gtcgcataac atgatttgac tgtccgccca tttccgtttc gtatatctcc
    13381 tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag
    13441 gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg
    13501 ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat
    13561 cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga
    13621 caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc
    13681 gtggcaggta ataaaaccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt
    13741 ttttaattct ccaaagtaac gtaagcctag cacagccgct gtattatgga gagcatcaaa
    13801 caaaatgagt ccattgggaa atgtttcatg tgcccactcg ggatcacaaa agatataaag
    13861 atctccctca tcaaaggaaa cagacttttg ataagtcttt aagatcttcg aattcgctgg
    13921 ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg
    13981 actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg
    14041 cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc
    14101 taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa
    14161 tactagtgtc ctgacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc
    14221 tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc tgtcaaaaac
    14281 ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag
    14341 tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc
    14401 tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac
    14461 tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata
    14521 aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac
    14581 cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca
    14641 cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg
    14701 ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat
    14761 tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca
    14821 actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct
    14881 gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt
    14941 aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca
    15001 tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat
    15061 tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca
    15121 ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt
    15181 ttttctccgt tttt
//
LOCUS       DANKHK010000060        15040 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000060 DANKHK010000000
VERSION     DANKHK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            494..826
                     /locus_tag="PW109_001760"
     CDS             494..826
                     /locus_tag="PW109_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2511578.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1445..1786
                     /locus_tag="PW109_001761"
     CDS             1445..1786
                     /locus_tag="PW109_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2511579.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            1900..2139
                     /locus_tag="PW109_001762"
     CDS             1900..2139
                     /locus_tag="PW109_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511580.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2206..2415
                     /locus_tag="PW109_001763"
     CDS             2206..2415
                     /locus_tag="PW109_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511581.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            2475..3146
                     /locus_tag="PW109_001764"
     CDS             2475..3146
                     /locus_tag="PW109_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2511582.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3199..5175
                     /locus_tag="PW109_001765"
     CDS             3199..5175
                     /locus_tag="PW109_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2511583.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGANSKYIKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEIVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGQFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            5190..5942
                     /locus_tag="PW109_001766"
     CDS             5190..5942
                     /locus_tag="PW109_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2511584.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5958..6719
                     /locus_tag="PW109_001767"
     CDS             5958..6719
                     /locus_tag="PW109_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511585.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGETKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNDEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGIKKNFFENCWDFVTHLFSRD"
     gene            6732..6992
                     /locus_tag="PW109_001768"
     CDS             6732..6992
                     /locus_tag="PW109_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511586.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6989..9079
                     /locus_tag="PW109_001769"
     CDS             6989..9079
                     /locus_tag="PW109_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2511587.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            9106..9570
                     /locus_tag="PW109_001770"
     CDS             9106..9570
                     /locus_tag="PW109_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511588.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSASVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            9567..9809
                     /locus_tag="PW109_001771"
     CDS             9567..9809
                     /locus_tag="PW109_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2511589.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            9827..10129
                     /locus_tag="PW109_001772"
     CDS             9827..10129
                     /locus_tag="PW109_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511590.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            10199..12262
                     /locus_tag="PW109_001773"
     CDS             10199..12262
                     /locus_tag="PW109_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511591.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTKSSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            12262..14871
                     /locus_tag="PW109_001774"
     CDS             12262..14871
                     /locus_tag="PW109_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2511592.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNMFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            14868..>15040
                     /locus_tag="PW109_001775"
     CDS             14868..>15040
                     /locus_tag="PW109_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511593.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLI"
BASE COUNT         5549 a         2301 c         3087 g         4103 t
ORIGIN      
        1 tacatttttt gtataaattt aattgtctgc tatgcttgtg atgaattgat tttcgttctg
       61 cacttttgga tgataaattt ggacaattga aaatacaaaa aaaagaaagg cggtatgaac
      121 tcggaaggct ggtaaccttc gtcagcgttc atacacggca ttaagatttg gcccttaatg
      181 cgttaacctt tcagtgttac ggtattaccc gcaatggatt tagtataaca gatattcaaa
      241 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
      301 atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg
      361 aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa
      421 tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagaaattg
      481 ggtggtttat tttatgtcat atacaggaat tttgagtctc aaagatattt gtcactacgg
      541 taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc aaagtaaaac
      601 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg aaatgattta
      661 ttacattggg aaacaaaagc aaataatttt aaaagatcct atccccttaa aagagctata
      721 tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg
      781 agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg atcattctca
      841 taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac
      901 ttagagtcac tagaaaaata aggagtagac ttgatctatc aacaattccc ttcggggagt
      961 gacggggtag gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt
     1021 gtttggatag tggtgaaatg aatttgtgta aataataagc gcagagtaaa caagagggaa
     1081 tttagaagtt agacaagtta gaatagcgtc tagattaatc atgagcagct actaagggat
     1141 ctagactttg acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc
     1201 acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg ttgattattg
     1261 gatcaatcga gcatttaccg ctgtctacta gggtttctcg tttcggacgc cataaacgag
     1321 tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga
     1381 ataaagtgaa aaaattgata taaaacatag agaaaatgaa atttacgtaa tggaggaatg
     1441 acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta aggtttcttt
     1501 ccaacgttgg gattacaaag gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt
     1561 agatgtttta gcattagacg aggatgattt atacgatcaa acattgactg ataatccaat
     1621 tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaaa tgacgcttat
     1681 gaatgataat ggagacgaat tatctgttga agatacttgg tcatatttga gtgattatat
     1741 tgtcagcttt gaaatcattg aatttgtagc ggataaagaa gaataaaaac tggaggaata
     1801 aattttacag ataaaggctt agaaggagtc acgacgatta ataaaaaggt agtattacct
     1861 taggcattga gtaaggaatt tatttaggag gaatagtaga tggcgcgacc agttttagag
     1921 gcgatggata tggatacttt gacaaaagga aggtatgtac agctgttagc tcgtggttat
     1981 tcacctaaag aagtattcaa agccttatct aaaggaacag atatggaaaa agaaagattg
     2041 cgaaaagaat tcgattattg gagaacccat aatggaatca aggatttgaa accagcacgg
     2101 ccagtaatta aacgaaagaa aaagataacc caagactgaa gtgtagagcg tatgtgatca
     2161 taggcttttt tatttttgca aaaaacaagg aaggtaggaa aactcatgaa ttatgaagaa
     2221 atcgaaaaca gaaaaaaagt gtcgaaagaa atggaagaaa agttactaaa aacgatgaag
     2281 caaaaacacc ttaaaagatt atctgtcgcg caatatatca atgatatgca gttaacagga
     2341 aaagagaaag cttgtttgtt aggttcaatg aaaaactttg aacagttgag acgaacttat
     2401 agaattcatt tttaaaactt tttctatgga aatgagaaaa agtttttttg ttttgtctga
     2461 aaggaggcag atggatgaaa aaatggttat ttggtttttt aggagtcact ttaatcgtct
     2521 tttgcagtgt gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa
     2581 atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg
     2641 atgcaacgaa cgtcaataat gatatgtctt tggggcaaat gttgtccata caaaaagaag
     2701 caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt
     2761 tagcattgcc aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt
     2821 atttttatga agatgccgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg
     2881 aaatgccagg tgttttattt tcagatattc aaaagttgtc attaggggag gtgatggact
     2941 tagttagtaa cgatggcgtc tatcgttaca aggtaacgtg taaatttatc gtatctgagt
     3001 actttaaatt aattgatggc gttcctgaag agaactcttt cttgtcttta ccaaagaaag
     3061 gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat
     3121 acgtggtaca gggagagcta caataacaag gttgtaatca acgcgagtcg ttgaaatatg
     3181 aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttgtt
     3241 ggaatgttag gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc
     3301 acgcaaaaaa cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa
     3361 ggaacgcaat tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa
     3421 tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta
     3481 ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa cgcgaaggtg
     3541 gacgaaatcg ttaaagatgt agaagacaaa ggtgctaata gcaaatacat taaaaatgca
     3601 accaacaaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc ggatcaacca
     3661 gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga
     3721 ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga caatacagga
     3781 tcaggatttt taaaagatat tcatttgtat ccaaagaaca ttgtttctaa gttgagtttt
     3841 gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc tttaaaaggg
     3901 gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg agatcttgta
     3961 tcggatgatc aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg
     4021 gaagttcctt cagaaattgt agtgggatca gataaagaac caacagccga tcaatattta
     4081 ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgac
     4141 ggtgtgacaa gcgatttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa
     4201 acagtaacaa atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat
     4261 caaggaacga ttcatatccc aacagacatt gcaggcggag cagacggtat cacagtaaac
     4321 ggcgtgaaaa gcgaaacttc tccttacagt gtatttaagt ggggggatac agcaggacaa
     4381 ggactttctt atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg
     4441 gtattaaaag aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat
     4501 tttatcgtga ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt
     4561 caaatgactt ataacatgta tgtaaatgat agcgcagctg ttgccaatcc attaacaaat
     4621 agcgtggatt tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg
     4681 aaagctgacg ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga
     4741 ataggaatca aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga
     4801 aaatattatg gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta
     4861 gacgatgtag caaatgcagc gatcctaaaa tcagataaag aaggtcaatt tgaaatcact
     4921 ggtttaactg aaggtgaata ttcattggaa gaaacaaaag cgcctgaaaa ctatcaaaaa
     4981 ttaacgaaag agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc
     5041 acgatcaaaa acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg
     5101 tatgtattaa tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt
     5161 aagaagaaag cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa
     5221 tcatgggtaa atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga
     5281 tgcctgctga agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc
     5341 gaacaggaac ggcagcagaa acggattcct ctttggatgt tgctaagatt gagttaggcg
     5401 atcctgttgg tattcttaca attccaagta tttctttaaa actaccaatt tatgacggaa
     5461 cgtccgacaa aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag
     5521 gaggaaacgg taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt
     5581 ttgatgattt accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc
     5641 agcacgctta tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact
     5701 ttgtcactta cttagaacca aatcccaatg aagatcgagt aacgctaatg acatgtacgc
     5761 ctaaggggat caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat
     5821 cagaattaaa agatgaagaa aataagaaac aaaaattatc ttggacatgg cttttaggaa
     5881 gtactgtttt tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat
     5941 aaaggagggt aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt
     6001 tttctttcac tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca
     6061 cagaacaaac aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg
     6121 attcgtcaac agttgattct tcagaagaac cgaaaattgc tgaagacgat caaatagtca
     6181 aacagaccat tcacgttcaa aaaattattt cttccgaaca attagacaac caggggcaaa
     6241 aacttgaaaa acaagaagga gtaaatggcg cgaagttttc tgtttatgat gtatcggaca
     6301 ttttacaaaa gatggatgtt aaggatctca ctactgacca aattgaatcc caattaaaag
     6361 atcgagtgaa aaaactattc agtgatcaat tgaagctagt ttctaatgga gaaacaaaga
     6421 caattgatca acaaacaggt gtctttgaat tctcgataga agtacaggca aatcagaaac
     6481 aagcttatta tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc gaggatattt
     6541 tgctattaac acctgtttct gataaaaacg atgaattctt aaaggatgtg tggatttatc
     6601 ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa aatcgtttct actggtataa
     6661 agaaaaactt ctttgagaat tgttgggatt ttgttactca cttgttttct agagattaag
     6721 gaagaaataa gatgaaactg atcaaattat tattattcaa tctttcttgt ttaaccgcag
     6781 tctttttgta ctgcggttta ttattttctc aattatctat tttaccaagc taccaactat
     6841 acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt atggaaaagc
     6901 tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga atttcactag
     6961 tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga caagacggtt
     7021 aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa aaacagccaa
     7081 tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg taaagaataa
     7141 ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa acggagcgtc
     7201 aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg actacgtagg
     7261 attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaagaat acggtgaaac
     7321 aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc aatattgact atggaaaaat
     7381 cggtacgtat aacgggaaaa aagtcaatat caagctagtt ctatctaata ttcacttata
     7441 ctcggatacg ttgccgtgga atctactaga taacaactat acaaaaactc attttcgaga
     7501 cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa cagtactttg
     7561 gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga atgtgcagct
     7621 agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat ttatcagttt
     7681 taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat atgcccacta
     7741 tgacaaaatg aatacgacag attggtatgt cagaaaagat acggttcttt cagaatttaa
     7801 atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct ctaagctgac
     7861 acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga agtttggtca
     7921 aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg gttcttctaa
     7981 tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc ctgatcaacc
     8041 tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg
     8101 agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg atttactagc
     8161 ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc
     8221 acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa
     8281 aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc gaatgaagta
     8341 taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa
     8401 tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg atacaaatat
     8461 cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg gaaaagatgt
     8521 aaacggacga aatgacggta agaagggtac gccgacagct cctttaaacg caggatctga
     8581 agtacaatat ctagtcacac aaaaatggca cacaaaaggc gtagacgctg cgtcagatca
     8641 ctacaaacaa tttagtattc aagatccaat tgaagctcgt ttgacatata aagaagtttc
     8701 tgcgcaagta atagataaga gtaaaggaaa agatattact tctgaaggca cactaactta
     8761 tgatagcaat tcaagaacgc tgaagtggga agcttccgct gacttcttaa gtaacaatct
     8821 tttagatgga cgagaaattc agctgatatt tacagctaaa actccattac aatcagaaaa
     8881 aaatattgat aaccaagccg tagtagcagt agataatgtt tctaataaaa caaatgttgt
     8941 gacaattggg gttgatccta acttgccaca ggtcattgtt cctaaaacag gttctacaca
     9001 tttagtaacg atttcagcgg tcagcttact attacttgtg ttagcaactt ttagttatgc
     9061 ggttctgaga tacatttaaa ataaaaaaac gaaagaatga ataaaatgaa aaagttaata
     9121 atagtagtta cttccttgat tgccatgatt gttattttag tcggatgtag taatgaaaaa
     9181 aataaacaaa ctaatcaaga taatggcgta ttgaaatcag gaactatgtg gaaagaagaa
     9241 gtaggtggtc ttgtttataa tttgaaaatt atagacgaga ctacctggga atactctgag
     9301 agtgtatggc atccagatcc tgtacaaata acagtcaaga ggcaaaaaga ctataaaggt
     9361 ttagaaagat acaaaatagt agattcagct agtgttaggg aatttataaa caaatctgat
     9421 tctttattta ttgttgttcc ctatgagaag aatggcgtaa aaaaaataat ttttcttgga
     9481 tctagcaagg atgaaaagcg aacgaaagag aaattgattc acgatggatc acaatccaat
     9541 aaatataagt tacaaaagac tagcgagtga ttgctagtct ttttatttgt cctgaaggag
     9601 gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac
     9661 gccgcggtgt atctgaaaca cagtggaaac aagaagtcct tgaaaaagca caatttgacc
     9721 tattacgggg aaaagacaag gacttagaag gatatatcta tcaacgtgaa cgtgaaaagc
     9781 ttgttttcga tgaaattatt aaacactaaa aggagcgaac aattttatgt ttgaatcaat
     9841 tacaatgaat tttttaacac aaccgaaagt agtagctgcg gttgatattt ttagagcatt
     9901 acaaactgcc gcaactagtt taaaaaacaa tctgatgatt tttgctgctg ctgcggccgt
     9961 tgcttccatt gttattgggg gtcttttctt cctatttgga cgaagaggag ctgaaaatgg
    10021 gaagatgcac gtaggctatg tattgatggg ggtaggagtt attttagcag cagcttctat
    10081 tgtggtttcg ttatttggat tgtttggaca aacacctcaa ggattataag aaagtaaaaa
    10141 agtaggagta agaagtagtc gatacttttt actccttttt tatgaaagga gaatactcat
    10201 gtactttacg attgcaggca ataccaaaac agataaagag ttaataaaaa aagcaacgaa
    10261 ggaagtagga aagcgtactt cttttttacc gaaaacaatt aaatattctg tcattgaaac
    10321 ggaagaacag ctacatgtgt tagggaaagt actggaacac gaaagtcgat cttctgttaa
    10381 gttgactctc acgttacatg acacagaagg aaaaatagag aaagctggta cagaatacgt
    10441 ttttggccca ctggattatc aaatttcaga aggtttctat cctacattga tggatttaat
    10501 ccgccttgat gtgtttaaag aaacggatga aactatgagt gtggaagagt cttatcgaaa
    10561 cgaaaaactt ttacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgaatca
    10621 agggagtgaa gtcgaagtaa atgaagaagt accatgggaa cgaccaatat ttccaaagaa
    10681 ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa
    10741 atcgatattt gagaaagaga acgatgtgat gggtcagttg gaggtagatc cctttgaaga
    10801 aacaatggag gatgaagaga cggccgaagt agaagttgat ccagttgaag ctaatgaaga
    10861 gtcaacatgg actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt
    10921 agaaaaaaac gaaatagaga tacaaaaggc aactgtattt gattatattt tggatcaata
    10981 tccgccagag catgaatgga tgaaagaaaa aaacaaatta tttttaaatg agctttattc
    11041 gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc
    11101 acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa attggcatga
    11161 gattgcacaa gaagatttat tagccacctt tgaagaacgt caacaagaaa cagatcaaga
    11221 aattaatgag tatgcgacgg aacagtcgtc taattgggag aaagcaaaag aggaactaaa
    11281 taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa attaaagagc gatacgaaaa
    11341 gaaacgcgac aatgcgtatt ccttgtgtgt agaaaagatc aataatttta ctcagacgaa
    11401 tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa gagaacgaaa
    11461 agaagaattt tatcaaggtt tgcgtacaga taagctaaag attagcaaag aattaaatga
    11521 gcaactgaat aagctctatc aaataacaag tgaagctctt atggaaaaac acgagcagat
    11581 ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca
    11641 agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgcg aacaattaga
    11701 actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg gcaatagaaa aacaacggtt
    11761 aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac
    11821 attgcgacaa gctcagttaa attatctagc acaacctcaa ccaacgcctt cagtagctgc
    11881 tgttcaaacc ccaatgaaca accaaacaaa tctaactcct tcaccacagc gttcctttaa
    11941 cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag ggacagtatt
    12001 tgcttttaat catatcaatc aatcttcaca ggctgattct gtagccacaa cggcgtcaat
    12061 caaggaagaa gcccaagaac aagcaagaaa agagtatgaa caacaattgg aagaattatt
    12121 gaatcgtacc aatcaggcaa caacaaagtc aagtatggaa aatagcaaag agagtgattc
    12181 tagtaaggct aaagaaggat caagcgattc aactgtacaa tcaaacactt ccgaaagttc
    12241 agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa cgagacgaaa agacaaacga
    12301 ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc ttcaggagaa
    12361 gagctaccag aaaaacatga caaacgtatt tctcaaaaga ttttttatag tggcttagct
    12421 tatcttcctt taaactatgt tgttacaaga ataccagaag catttcatcg atttgagatt
    12481 attccaggaa gtttacaagg agtgaatatg tttcagttac aagaaatgcc gcagttattc
    12541 cattttgatt caactggttt ttggaatcct ttgaccccaa caacaagttt aggcacacgt
    12601 ttagttgttt ctacgatttg tgttgttcca gctgtactta tgggattgaa aatggacatg
    12661 tggtttcgtc ctctagcaga tgggcaaaaa ggagataacc gattaaaaac gttaaaggaa
    12721 atccaacaaa tttatccgca aataccagaa tcaaagggag gttttcctgg ttatggcgga
    12781 ataccaatta ctcattataa aaagtactgg tatattcaga cggatacagt aaatacttgt
    12841 attgttggta cttcccgttc agggaaagga cagatagaag ttttagcaac cattgacaat
    12901 ttgtctcgtg cagaaagaca aagttcaatg gttgttaatg atccaaaaac agaattatat
    12961 gttgctagta aagatgaact agaagctaga gggtatgatg tgtatgcatt taatatcacc
    13021 gatccactgc aatcaatgag tgataatcca ttggcactca tcgtgaaata ctggaagcga
    13081 ggagacatag atactgcaac gcagttaacc aatactttta cgaatacgat ttattatgat
    13141 gccaatgctt cagacaacaa atactttaac gaaaatgcgc agaaagctgt aaatgcgctt
    13201 atttttgcgt tgttagaaca agccgatcaa acaggggatt actccaaatt aacacccaat
    13261 aatcttgttg agcttctttc tgaaattggc ggttttaatt atcaagatcc tgagaatgat
    13321 tacaaacaac tcaatgcctt agatgaattc atgaatcagt taccacctgg aaacatagca
    13381 aaaaaacaat atggggcaac aaaaattggt tctgataaag caaaagggaa tattttatcg
    13441 acggcaatca caggattgaa tccattcacc ctaacgaaaa ttgcgaaaat gacttctcaa
    13501 tcaaccattg attataagtc cattggtttt ccgaagtatt tggatctcaa actacacgag
    13561 agtctgttaa atcagcgaat tgttgtagag ttttatcatc aagggaaaaa atttcatgag
    13621 gaacgtgtca aagtaggtta tagaggtttt tgtgaaatca actttgattg tcatttagag
    13681 cagggagatc atgtttgctt aaaatacgat catcaaggaa aagaatatgt tgcttggtat
    13741 gagttttctc aaagagtatt gaaagacgaa gagggaaata tcgtttatcg aaaaaaacga
    13801 ggagaaaccc atttaccaga attagaaaaa gaagctgttc taacattaga tgaggataaa
    13861 agtaacttat tagtagagtc tattcggatg cactataccg ataaaccaat ggctattttt
    13921 atgctcacac ctgattatga tccatctaac catatcattg ttagtatttt tcttagtcag
    13981 ttgtataaag aattatctac gcaatgtgtt aagactaaag gagataaatg tcaccgtagg
    14041 attcatttta ttttagatga atttgggaat atgccaccaa tggataatat ggaaggaatc
    14101 atgacagtca cagctggtag aaacatgttg tgggatctgt ttattcaatc ctatcagcaa
    14161 ctttatgcga agtatgggga acaaaacgca gcgattatca aagaaaattg tcagacacat
    14221 atttatatta tgtcgacaga cgataatacc atcgaagaat tttccaaaaa agtggggaat
    14281 aagacggttg aacaagagaa cagtaccacc aattcattag gtatgaatac tcatatcaat
    14341 cgcaatgttg attcagatcg tatcttaact cctgaaagaa tttctacttt tttagaaggt
    14401 gaaacgattg tactcgctcc tttaaaacga caagatttga aaggtcataa aatacggccg
    14461 tatcctattt ttaatcatgg ggaaacgaat cttccttatg cttatcaata tattgaagat
    14521 ttcaatccat cgtgggatat caatgatata gacatacatt gtacacatgc ggacttggat
    14581 cttaaggctt tacaaattga ttacgattat tttctacctg atatctatgc acgtcgactt
    14641 tatcacaata tgcacgatca aatagaagag gaagctaaag aaaacatttt tattaaaatc
    14701 gttcaatctt tagacaactc aacggcacaa aagttactta ttcaatttta tgaagagaag
    14761 caaatagatc gtttgcaaga ataccttgaa aaattgcatc aaaatgaaga aattacagag
    14821 gttataagga aacgactttt gctagaagct gaaaaggcgg tgaaagaatg atcattggag
    14881 gaatattttc cggcattgcc gactggtggt caggcttatt tggggatggg gatgcagatc
    14941 ccaaaaccgt aacgaaagtc ttacaagatt atggtaatta tctgcagtat caagatgtat
    15001 tcagttggat ttgtaatact tttttatggg gtttgattaa
//
LOCUS       DANKHK010000061        14596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000061 DANKHK010000000
VERSION     DANKHK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            414..1964
                     /locus_tag="PW109_001776"
     CDS             414..1964
                     /locus_tag="PW109_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511594.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEA
                     HEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERAS
                     KDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRK
                     RKDPIVLN"
     gene            complement(2040..2375)
                     /locus_tag="PW109_001777"
     CDS             complement(2040..2375)
                     /locus_tag="PW109_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511595.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2502..2717
                     /locus_tag="PW109_001778"
     CDS             2502..2717
                     /locus_tag="PW109_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511596.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2753..3115
                     /locus_tag="PW109_001779"
     CDS             2753..3115
                     /locus_tag="PW109_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL2511597.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWIPMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3161..3487
                     /locus_tag="PW109_001780"
     CDS             3161..3487
                     /locus_tag="PW109_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511598.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYSLNRTCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYT
                     EVLMLN"
     gene            3974..4111
                     /locus_tag="PW109_001781"
     CDS             3974..4111
                     /locus_tag="PW109_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511599.1"
                     /translation="MAHNERFKQEIIQELEKGVQHQKLISKTAQKFDISLAKEVKLHE
                     V"
     gene            4176..4958
                     /locus_tag="PW109_001782"
     CDS             4176..4958
                     /locus_tag="PW109_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511600.1"
                     /translation="MKNSSNSAEVNVSTSTSEAVQCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLYVYFSFEYDGVKYSEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKKVSGENNETEAVTEQTMDERIDSSESNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNV
                     VTEQVGKNVPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            5459..6649
                     /locus_tag="PW109_001783"
     CDS             5459..6649
                     /locus_tag="PW109_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL2511601.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFKGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGAFAEKWKTSYPKMVESILSNDHLLTFYDFPFAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(6911..7654)
                     /locus_tag="PW109_001784"
     CDS             complement(6911..7654)
                     /locus_tag="PW109_001784"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013539.1,HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2511602.1"
                     /translation="MALIKYFPNADTQLTYAESLILDYLEDHLESISSLTITKTAEDN
                     NVSTTTVVRLCKKLKVNSFSRLKFLLEEIYQEQNLSVSYDFYDSIERNFNETLKQNSN
                     STIEEVSQLLKDRTQIYIFAIGLSKACGDYLELALQQLGLNCSLIYDHQVIWNFDRPT
                     IPNSLAIFISNSGESEELISLLNRIKENEDLTTLSLVNQPNSQMEKLTDYSLHGYVAP
                     VVINGSDFSPHFSLILLIDLVIQGYLKTN"
     gene            7795..9777
                     /locus_tag="PW109_001785"
     CDS             7795..9777
                     /locus_tag="PW109_001785"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF014368.1,HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2511603.1"
                     /translation="MKLSELTTNDLIMLDKDYASKDDVFKAVAEELLIQGIIQDKKVF
                     INALYHREAESPTGLENGIAIPHAKSDTINKPVFVIIRLKNSINDWESLDSSNKVSLI
                     FLLAIPKQQTGTTHIDLLAQLSRSLMNPEFVNGIKNANTSEEVMTLLELTNKQEPKEE
                     IRESKGLVLGVTACATGIAHTYMAAEALEKAGAELGYEVIVEKQGANGIEDRPALSDV
                     GRAKGLIVATDIEPQNMAHYENVPYIKTKVAAPIKDATSLIKQVIEQPAGNYHSEQSV
                     DSNISEKKSVGTELYQSLMSGISYMIPIVVGAGLMTAIPQIGGMFFGIEGGTIGDAIN
                     ATSSNGAVSFLYYLNSFGGFIFSLMYPVLAAFMANAIAGKVGMTAGFIGGGLAGGMIY
                     ALTGQENQTPSGFLGAIAIGFVAGYFCRFLNSKIKLPKNIQSLKPMLIIPLLSILLVY
                     VLNLYIFEPIGGGLNNLLFDVISKSTGTYVIAIVIAMATAFDMGGPVNKAAFAVTMTL
                     AANQSFPMTANILGCIIPPLGIGLSVILNQYVFKRDIYDESMEADGYTSFISGIIGIS
                     EGAIPFALKNPLITIPLNLLGSALAAVLATLFGVEIWFPIPQFWGWPLVQGLVPYLLS
                     LLAGVLFVAIGNIVIRDYLATKKKKQVEKNDNWVRS"
     gene            9755..12355
                     /locus_tag="PW109_001786"
     CDS             9755..12355
                     /locus_tag="PW109_001786"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013256.1,HMM:NF020822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2511604.1"
                     /translation="MTTGYVVNQTHWDREWYFTNADALILSDEVFSDVLDELEQHPEA
                     NFCLDAQSSILNEYLELYPENESRVKELIAKQQLFIGPWFTQSDCLLVDGESLIRNAI
                     VGVRDCKRYGEAMPIGYIPDTFGFNAQMPTLLRQIGLDNLIFWRGIDFSKQVSTPYFK
                     WKGLGEKEVYAMNIPQGYGCTPNMQTTQEYIDNRMDGYIDFVNQFQEHEDILVPAGGD
                     QQAIVRQLQSKLKEINKKSKHTYKISTFPEFLEKVKEKKNLPVYQGEFRLPAYARIHR
                     TISSVRMDIKLANYQLEQKLLRRIEPLYVLAQKYEIQLSQELLIKVWKKVLASQAHDS
                     LGGCVYDNVAADILHRYKEAEEMADGIENVITKKLARKLRLTENQVLVINTAAKTRNK
                     YFKIKVISQSKNIRFPQAEDAWIAHEKKYTAREDILIEKPEGKFYLTEPEYLELDVMV
                     KLSVPGLGFKVLNFEESTKPMAEYQSIKSGDDVAIKNDFIELRFASGHLSATFQGEKI
                     TDFIRLVDCANDGDTYDFSPLRGDYEVDLPFDYAEKKATRHSQKLIIYGQTALPFDLA
                     DRQNPKGDHRVVEYQVTLKMNDDSSVIEGSVEIDNQVLSHRVRLVFDPNIKQGDTYEG
                     VTAGHLKNNRVDEVNIDSYPEYPAPLNIFDKTLSLANQERAFDFYGHGLKEYEHSEDK
                     IFITLFASTGQFGKPDLLWRTGRASGDTTNQGHVMHETPLAQFSGLRSFDFAFDLHQS
                     SFDEKHVEETLSIISEQTISYQAQTLNHFIYRLDNKIQEREESLLELQEDGLLELDGL
                     TVSAIYPSFFEKDKKYIVRLSNATGSDVRIRKEHFEGKHPVIVNALEEEIDQNFVVKP
                     YDLLSVKLSY"
     gene            12373..13353
                     /locus_tag="PW109_001787"
     CDS             12373..13353
                     /locus_tag="PW109_001787"
                     /inference="COORDINATES: protein motif:HMM:NF013567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2511605.1"
                     /translation="MEILNWGIAGTGFAANDFVSKIDRKKYSIVAAFDIQPQALDNFT
                     KLHQIEKQYTNFEEMVKNPKIEGVYIGTPNQTHYPLMIQALQAGKHIICEKVMTLNSA
                     QLAHVQSVAKEKQLVVIEGITLFYMPLFSKIKQMIELGMLGKISGVNVTFGSMKEFNA
                     TNRFFDRKKGGGALFDIGTYALSAVVYFLGTQTKLLNTEVVMSETGVDEKSVFSLINE
                     DGILASAIISFRGKMPKQIIISGEKAYITINDFPRAEEAVIFWNDGRMEELKFGDERD
                     IFNFEVETVNRYLKSDSNEMDVREVTKCVIGIMDQAREQWGLTYPNEELV"
     gene            13461..14458
                     /locus_tag="PW109_001788"
                     /pseudo
     CDS             13461..14458
                     /locus_tag="PW109_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         5031 a         2232 c         2996 g         4337 t
ORIGIN      
        1 tagatcaaga aaaattatta gagaagccag ttacaaaaaa agaaaaaact gatgtaagag
       61 ataggtgttt agataactat agagaagtta caagagatga gtttgtagag atactaaatt
      121 catcacaatc atttattcca agcaagacaa agaataaagg caatgacaag gagagttttg
      181 tggaaaccag agtaattatt ctggatgtcg ataacactgt taaagatgag aaggggaagg
      241 tagtagattt gagtaaagat gatagtagat acttaagcgt agaaaaggtt ttatctatca
      301 atttagttca taattctgcg tttgcgatac aaaaaagtat tcggtactct gaaaatctag
      361 aaaaatacaa gattgtcttt ctattaaaag aagcaattac tgactatggt aaaatggttg
      421 ctgtttacga atacttgaaa aagaacatcc ccgggtgtga tgacaatgta aatgcttcga
      481 atagaatgtt ttttggagga tataagtcag atgcagtgat aataattaat gaagacaaca
      541 tgttagatat tactgagctt ccgattgatt ttaccatgac atgttctaaa aattgtcaaa
      601 gtgattcagg gagagagtat gaactagtca atgaaaccga ttttgttaag ttgataaaaa
      661 atgacgacaa agaagaaatg aaacagtggt ttaaagactg cctgtttgat tcctccgaca
      721 tgtcttttaa tgagatttat gaaagattgt taaccattga tatgaataaa ttacttaagt
      781 caagtaaaaa cttaagatgt ctgtttcaca acgatcataa tccctctgct agtattttta
      841 ccagtaaaac tgggcattcc attttttatt gtcattccag taattgtgat atcagtgaaa
      901 attttattgg agttgtgatg ctaattttaa agaaagacag tcgggtagaa acatttaatt
      961 ggctattaga gaacctagac ttgtatccta cacattttcg aaaattgaaa gaggaatcaa
     1021 aagtactttt tgacactctt gaatcaatga aagacaaact tttcaggaca ataaggaatt
     1081 atctagagga aatgcgacgt gtctatgatg tcttgctttc tgatgttaat ttttttgatg
     1141 atagtaagga aagtgtgact tgtattttgt cgggggaaca attagctaaa aaaatgagtt
     1201 cttactatgg taaaacttat gacatagata aatgtaataa gttactatca cttatgactt
     1261 ttataggatt gataaaaaaa ttagatgatg aagatgttcc tttgaagatg ctcgaatacc
     1321 taaataggtt aaaggaagag aaagcgttag gaaactttaa tcataagaga agcaatgtct
     1381 atcgattaga cgtttttaat gcaacttctg tgattcaaaa attgaatgaa gatatccttc
     1441 cacttctgga aaaaattcat tttacatatc aatatttttc ttatgaatgg gttaagatat
     1501 gttttaacga aaaagaggct aaaagagtat ttcctcaaaa ttcccgatca gaactcagca
     1561 aggaaaaaca attgatttta gatgaagcac atgaagtttt acagggctta tcgtggcaag
     1621 ggacatatct aatgacagag aaagaactta ttaacactgt ttgtgaacgc ctacgtaatg
     1681 ttggggataa cagcgtgaaa aatgctttaa taaaaaacag aggctacttt gttaaacaag
     1741 gattcactct tgaacgagct tctaaggata ttaagaaact atacaatgtg aaagcaagta
     1801 gtagtttttt aatatattta gcgaaagggg cagggataga tgaaacaata aaagaattga
     1861 tattatcagg agctgggata gttcctctgt atcaatttac aagtgtaaca agagatgatg
     1921 gacggagagt aaggaagaga aaagacccaa ttgtattaaa ctagacaggt tatccatagc
     1981 tcttaatctt atttcatctc aatacaccca ccgttaaagt tcggtgggtt atttattttt
     2041 caatctttca atagctgatt gcgttcattt tcttgccttt ttaatagctt catatagtca
     2101 attgttcgaa ctgcatcttc agtgctaaga gaacgataga gagccaaaaa ctcttcctca
     2161 taatctacga caacattgtc tagaaaatgg cttacagggg ttcctagggc tctagaaaga
     2221 gctgttaagc tatcgaggtt aggctctgtt acagaatttt cccatcttgc tactgcctgt
     2281 tgtgtagaga agattttttc agctaattct ttttgtgtcc acccgagagc ttttcgcttt
     2341 tctctaatga cctttgcata ttgtttctgt ttcattaata cacccccatc tcagtatagt
     2401 acaacaagtt gttgtatttg taatggtatt tgaagttttt tatattttat gagttgaaat
     2461 acatcatatt gttgtatata ataaaacagg aggggagaat catgaaaaaa gtaagtaatc
     2521 tcagccgata cagaaaagaa aaaggacttt ctcaaacgga gttagcaaaa aaaatgaatg
     2581 ttacacagca atgtgtaagt tcgtggcaaa cagggagaac gattcctaaa ccctatcaaa
     2641 tgaagatgct ttctgagata ttaagtgtac caatgaatga gctgttttct gatgtattta
     2701 ataaagtaaa cagttaacta ataagcaatt gaatgatttt agaggagaag acatgtattg
     2761 tagaaaatgt ggcaaagaaa taacagatga aagtaagtat tgccagtttt gtggagcact
     2821 acaagaaaca gatgagacaa ccggaacaga cacagtaaag aaaagtactg agcagacaat
     2881 taatgaaatg aaggaacaaa tcgtacatgg acaagagaca gggaagctgt atttgattat
     2941 tggttggatt cctatggtag tatcgttagc atttattcct gttgtatttg gtgctgttgc
     3001 tgttattatg ggttacttat atcgagagaa agatgaaaaa atgggtacaa ttttgatgat
     3061 agcaggggta gctggagcca tctttggtgt acttttaggg atgtcaatgt actgataaga
     3121 gggataagag caaattattt tggagatagg agataaaaag ttgaataaag taaatatgaa
     3181 caagaagttt gtcattatag gtattgttgt cttggctatt ataggtatca ttttttcaaa
     3241 aatgacaggt acatctggat tagagaagtc atcgaaacca ttggtaacac agattttaca
     3301 agaacagtac agtttaaatc ggacatgtga tgatgtgacg attactcatg aaaatggaga
     3361 taacacatac cgagctaaag ctatattaga caatggaagt gctatcaaca taaatattga
     3421 gtactatcca aagaaagacc gtatatatgt agagatacca tatactgagg tgttaatgtt
     3481 gaattgaacc ccaccccatt gatgattgac cgtattttgc atggaaacct tgggaggcat
     3541 aagctaacta ggggttcctt tttcttttct ttttttggaa tctatttaag tgaaatgtat
     3601 ctgaaatgaa atgggagtaa gtgttataat agttaataca taagcgcttt attttatttc
     3661 agatggtgaa tagtataagg aatttttttg atattattac agtttatctt actataaagt
     3721 actatttttt aaagcaataa cagatgaaaa tttgattcat tcataacaac atgtacagct
     3781 ataccaccta cttggaataa ctgaagaaaa tgtacgaaaa accatttcaa gtaaacccca
     3841 aatgattgtt aatgaactac aagggtggtg aagcttcatg agtctccaca gagatgctcc
     3901 atcaaaagtt ccgtcaagct catgaaaatt gtgaaaaacg ttttgaataa tatcaaagaa
     3961 aaagggaatg gaaatggcac acaatgaacg ttttaaacaa gaaattattc aagaactcga
     4021 gaaaggagta caacaccaaa aattaatatc aaagaccgcc caaaaatttg atatctcact
     4081 ggctaaggag gttaaacttc atgaagttta acctgctagc tctactaacc gtggtaacgc
     4141 tcgtttttgt tggttgtact aaggctcctg attccttgaa aaattcttct aacagtgcgg
     4201 aggtaaacgt ttcgacctct accagtgaag cggtacagtg ttcacaagaa actactagct
     4261 tgccagaaga gctacttgga gattggcaga ttaataataa ggatgctggt atagagatat
     4321 cagacagtaa agctgtattc cgtataggaa ggtctcaagt agagtcaaag gactattatg
     4381 tagatgtaaa caacaaaatg cttttatatg tctatttttc ttttgagtat gatggtgtga
     4441 aatacagtga agtctctatt gaaagaactg gctatagtga atttattagc aaaagcgtgg
     4501 gaattttaac tatgcatact aaagatggac aatatataag tttgggtgtg aagaaagttt
     4561 ccggtgagaa taatgagaca gaagcagtta cggagcagac gatggatgaa agaattgatt
     4621 catctgagag taatgagaca gaagtagata ctgggaagac gatagatgaa agaattgatt
     4681 cattcatcga aacatacaca gctataccac ctacttggaa acttgtaaat gaagataaag
     4741 gaatcacgat aaatactgat acagagattt ctacagatgc tcaatcttta tcagatttat
     4801 tagccaacct aacaggaatc agtaatgtgg tgacagagca agttggtaaa aatgttccac
     4861 ttcggttact tgaaaatggg aaaaaagatt attctgtggt atttaagaac ggacaggtaa
     4921 ctgattacaa cgaagtattt aaaaaagtta ttgaataatg cttcttcaat ctttttcatt
     4981 catttacccc accccgttgt tatataaccg tatctcaaga ggaaccatta gaaagcatag
     5041 gctaactagg ggttcctttt tcttttgttt ttttggaatc cgcttaagtg aaatgtatct
     5101 gaaatgaaac gaaaataacg tgatttttta ctgtaatatc aaggttttag ctgttttaga
     5161 taccatgtaa cccaaaatag tttattgaga aaataggaga gtatgtgata ctaactagta
     5221 gggaaaaatg agataaaaag taaatggaat catttcagaa ataagtgaaa taagacaacg
     5281 taataggtat aacatgacta ttacatagtt ttagacatga aatattacta caaaatttgt
     5341 ctaattatgt cattatttat ggaggagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     5401 aagtcccttc tgtagaatag agttaccaca acacattcac agaagagagg acttccctat
     5461 gaacgatttt actacagaaa ttctaaagac tctagcgaac aaaggcgatt tgaatgaatt
     5521 attccgtgtc catttggaga aagcagtcaa tacgcttctc aaaacggagt taacggcttt
     5581 cctagattac gaaaagtatg atcgcattgg ttttaacacg ggtaattctc gtaacggctc
     5641 ctatgaccgt acggtcaaga ccgagtatgg ggaacttcat ctccagattc cgcgcgaccg
     5701 caatggtgag ttcaagcaac agactgttcc tgcttataga cggacgaatg acacgttaga
     5761 ggagaccgtc attcacctct tccgaaaagg tattaccatg tcggaaatcg cagacttgat
     5821 tgagaaaatg tatgggcatt actacacgcc ccaaaccatg tccaatataa caaaatcatt
     5881 tacagaagag gtaacggcgt ttaaagggcg ggagcttcat gaccgttatg ctgctattta
     5941 tatggacgca acgtatattc cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat
     6001 cgcagttggc attcgcccgg acggatcaaa ggaagtattg agctatgcga ttgcaccgac
     6061 tgaatccatc acgatttggg aggaaatttt attggacctt caagagcgcg gtttgaaaaa
     6121 tgtcctcctg ttcatcacgg atggcttaaa ggggatggta ggagcgatta gtcggttcta
     6181 tcccaaagct cgttttcaac attgttgtgt acacgtttcc cgtaatatca gtcacaaagt
     6241 gcgtgtcgat gatcgtaagg aagtctgtga tgattttaaa atggtgtatc aagcctcatc
     6301 taaagaggtg gcgttggaag cacgtggtgc ttttgcggaa aaatggaaaa ccagctatcc
     6361 aaaaatggtg gaatcgattc tttcgaacga tcacttgctc actttctatg atttcccctt
     6421 cgccatacgc aagagtattt attctacgaa cttgattgaa tcctttaata agcaaatcaa
     6481 gaaatacagc caccgcaagg aacagttcca aaacgaagag tcgatggaac gtttcttagt
     6541 ctcgtctttt gatacttaca accaaaaatt cctaggtcgc agtcataaag gctttcaaca
     6601 ggccgaaggc gaacttgaac aaatgctaag ccaactgatt gagaattaga aacaatctgc
     6661 agtagggaag aatcatttac acaaaattat tgacgctccc gtttaggggg gcagaaataa
     6721 atgatttgaa aatgtatgct gatttagata gtttttcctt atcccaaatg aaagtacaag
     6781 atgaaattaa ctaagacgta tagaaatgta gtttatactc ggaaagaata ctaatttttt
     6841 gtttgctcga agcataataa aagcagtact tattcaagca aattgagaaa acaaaaatcg
     6901 catgaaagag tcaattagtt ttgagatagc cttggataac aagatcaatt agtaggatca
     6961 aagaaaagtg tggagaaaaa tctgatccat tgattacaac cggggcaaca tatccatgaa
     7021 gtgaataatc cgtcaatttt tccatttgac tgtttggttg gttcactaga gataaagtgg
     7081 tcaaatcctc attttctttg atgcggttca gtaaggagat caattcttcc gactctccac
     7141 tatttgaaat aaatatcgct aaagagtttg gaattgttgg ccgatcaaag ttccagataa
     7201 cttggtgatc ataaattaat gaacaattta gccccagctg ttgtagagca agctctaagt
     7261 aatccccaca cgctttgctt aagccaatcg caaaaatgta aatttgcgtc ctatctttta
     7321 atagttgact aacttcttca atagtcgaat ttgaattttg tttcagcgtc tcgttaaaat
     7381 ttcgttcaat ggagtcgtaa aagtcataac ttactgaaag attctgttct tgatagattt
     7441 cttctaataa aaacttcaaa cgtgagaagc tgtttacttt tagttttttg cagagacgaa
     7501 caacagtagt cgtactaaca ttattatctt cagcggtttt cgtgatcgtg agagaagaaa
     7561 tactctctaa atgatcctca agataatcaa gaatcaatga ttcagcgtag gttaattggg
     7621 tatctgcatt aggaaaatat ttaatcaagg ccatattttg ttcctccaat ctcgagtatt
     7681 atatcacaat gactaataaa gtaaaatttt actccgattt agtaaaagcg ctttcttacg
     7741 gagggtgata tatttttgtt gtaccatcat aactaaggaa agtaggagat gaaaatgaaa
     7801 cttagcgagc tgactaccaa tgatctaatt atgctagata aggattacgc tagtaaagat
     7861 gatgtgttta aagcagtcgc ggaagaactg ttgattcaag gtattattca agataaaaaa
     7921 gtatttatta acgcattgta tcatcgagaa gcagaaagtc cgactggatt agaaaatgga
     7981 attgccatac ctcacgcaaa atcagatacg atcaataaac cagtttttgt tattattcgc
     8041 ttaaaaaatt caattaacga ttgggagagt ttggattcat caaataaagt ttctttaatt
     8101 ttcttattgg caattcctaa acaacaaaca gggacaacac atatagattt attagctcaa
     8161 ttatcacgat cgctaatgaa cccggagttt gtaaatggga ttaagaatgc aaatacttca
     8221 gaagaggtta tgacactttt agaactcaca aataaacagg aaccgaagga agaaattcga
     8281 gagagtaaag ggctcgtatt gggagtaaca gcgtgtgcga caggaattgc tcatacctat
     8341 atggcagctg aggcgttaga aaaagcaggt gcagaattag gctacgaagt aattgttgaa
     8401 aaacaaggag caaacggtat tgaagaccga cctgcactat cagatgtggg aagagcgaaa
     8461 ggactaattg tcgcaacaga tatagaacca caaaatatgg ctcattacga aaatgttccc
     8521 tatatcaaaa ccaaggtggc agcacctatt aaagacgcta cttccttaat caaacaagtg
     8581 atcgagcaac cagcaggaaa ctatcactcg gaacagtcag ttgattctaa tatttcagag
     8641 aagaagtctg taggaacgga actttaccaa tcattaatga gtggtatttc atatatgatt
     8701 ccgattgttg ttggcgcagg attaatgact gccatcccac aaatcggtgg tatgttcttt
     8761 ggaattgaag gtggaacaat tggtgatgcg ataaatgcga cgagttctaa tggagcagtt
     8821 tcttttctct attatctaaa ttcatttggt ggattcattt ttagtttaat gtacccagtt
     8881 ctagctgctt ttatggcgaa tgcaattgcc gggaaagtag ggatgacagc aggatttatt
     8941 ggcggtggat tagcaggcgg tatgatctac gcattaacag gacaagaaaa tcaaacacca
     9001 tcaggcttct taggagcgat cgcgattggt tttgtagcag gttacttctg tagattcttg
     9061 aacagcaaaa ttaaacttcc aaagaatatt cagtcattaa aaccaatgct gattattcca
     9121 ttactaagta ttttattagt ctacgtattg aatctgtata tttttgaacc aattggcggt
     9181 gggctaaata atttattatt cgatgtgatt agcaaatcta caggaaccta tgtgattgca
     9241 attgtgatag ccatggcaac agcatttgat atgggtggac cagtaaacaa ggcagccttt
     9301 gcggtaacga tgacattagc tgcaaatcag tcatttccga tgactgccaa tattttagga
     9361 tgtattattc cgccattagg aatcggactg tctgtaatct taaatcaata tgttttcaaa
     9421 agagatattt atgacgaaag catggaagca gatggctata cgtccttcat ttcaggaata
     9481 attgggatta gcgaaggagc gattccattt gccttgaaaa acccgttgat tacaattcct
     9541 ttgaacttgt taggatcggc gctagcagca gtgttagcaa cactttttgg agtagaaatt
     9601 tggttcccga ttcctcagtt ttggggttgg ccgctagtcc aaggtttggt gccatatctc
     9661 ctttcattat tagcaggagt gctatttgtg gctattggaa atattgtaat tcgtgattat
     9721 ttagcaacta aaaagaagaa gcaggtagaa aaaaatgaca actgggtacg tagttaatca
     9781 aacacattgg gatcgagaat ggtattttac aaatgcagat gcacttatac tgagtgatga
     9841 agtgttttct gatgttttag atgaattaga gcagcatcca gaggcaaatt tttgtttaga
     9901 tgctcaaagc tctattttga atgagtattt agaactatat ccagagaatg agagcagagt
     9961 gaaggaacta attgctaaac agcaattgtt cattggacca tggttcactc agagtgattg
    10021 tttgttagtc gatggagaat cattgatccg aaatgcaata gtaggcgtca gagattgtaa
    10081 gcgatatggt gaagcaatgc cgataggata cattccagat acgtttggct tcaatgcaca
    10141 gatgccgaca ctactgcgac aaataggatt ggataatcta attttctggc gaggaattga
    10201 tttttcaaaa caggtgagta cgccctattt caaatggaaa ggtttaggcg aaaaagaagt
    10261 ctatgctatg aatattcctc aagggtacgg atgtactcca aatatgcaaa caactcaaga
    10321 atatattgat aatcgtatgg atggatacat tgattttgtc aatcagtttc aggagcatga
    10381 ggatatttta gtccctgctg gaggagatca gcaggccatt gtccggcagt tgcaatccaa
    10441 actgaaggag atcaataaaa aaagtaaaca cacctataaa atcagtactt ttccagagtt
    10501 tctagaaaaa gtaaaagaga aaaagaatct acctgtttat cagggagaat ttcgattacc
    10561 tgcctacgct cggattcatc gaacgatttc atcagttcgt atggatatca aacttgctaa
    10621 ttatcaattg gaacaaaagt tattgagacg gattgaaccg ctttatgttt tggctcaaaa
    10681 atatgagatt caattatccc aagaactgct tataaaagta tggaaaaaag ttcttgcttc
    10741 acaagctcat gatagtttgg gaggatgtgt ttacgataat gtcgctgcgg atattttaca
    10801 tcgatataaa gaagcagagg aaatggcaga tggaattgaa aatgtaatca ctaaaaagtt
    10861 ggcaagaaaa ttacgactaa ctgaaaatca agttcttgtg attaatactg ctgcaaagac
    10921 acgaaataaa tacttcaaaa taaaagtaat ctcacaatca aaaaatattc gctttccaca
    10981 ggctgaggat gcgtggattg cacatgaaaa aaaatatacc gcacgagaag acattttgat
    11041 tgaaaaacca gaaggaaaat tctatttaac tgagccagaa tatttggaat tagatgtgat
    11101 ggttaagtta tcagttccag ggctaggttt caaagtattg aattttgaag agagtactaa
    11161 gccgatggcg gagtatcaat cgataaaatc gggagacgat gtcgcaataa agaatgactt
    11221 tatagaactt cggtttgcga gcggtcacct ttctgcaacg tttcaagggg aaaagattac
    11281 cgattttatc cgattagttg attgcgccaa tgatggagat acttatgact tttctccatt
    11341 acgaggagac tacgaagttg atttaccttt tgattatgca gaaaagaaag caacgagaca
    11401 ttcacaaaaa ttaattatct atggtcaaac cgcattgcct tttgatttgg ctgatagaca
    11461 aaatccaaag ggcgaccatc gagtagtcga atatcaagta accttgaaga tgaatgatga
    11521 cagctcagta attgaagggt ctgtagaaat cgataatcag gtcctatctc atcgtgttcg
    11581 tttagttttt gatccgaaca ttaaacaagg agatacgtat gaaggtgtga ctgcgggaca
    11641 tttaaagaat aatcgtgttg atgaagtgaa tatagatagc tatccagaat atcctgctcc
    11701 attaaatatt ttcgataaga cgctttccct agcgaatcag gaaagagctt ttgattttta
    11761 tggccatgga ttgaaggaat acgaacacag tgaggacaaa atatttatta cactattcgc
    11821 cagtacaggt caatttggca aacccgattt attgtggcga actggaagag cgagtggtga
    11881 tactacgaat caaggccatg ttatgcacga aacaccattg gcccagttct ctggattgcg
    11941 cagctttgat tttgcttttg acttacatca gagctcattt gatgaaaaac atgtagaaga
    12001 aacgctgtcg ataatctctg agcaaacgat tagctatcaa gcacaaacgc tgaatcattt
    12061 tatttaccga ttagacaata aaatccaaga gcgggaagaa tcgttattag aattacaaga
    12121 agacggactg ctggagctag atggtttaac tgtttcagca atttatccat ccttttttga
    12181 aaaagataaa aagtatattg ttcgtttatc taatgcaaca ggatcagatg tgcgaataag
    12241 aaaagagcat tttgagggga aacacccagt aattgtgaat gctctagaag aagaaataga
    12301 tcaaaacttc gtagttaaac cttatgatct attatcggtt aaattatctt actaattatt
    12361 gaggggaaac agatggaaat tttaaattgg ggaattgcag gtactggttt tgcagccaac
    12421 gactttgttt caaaaattga tcgtaaaaag tattctatcg tggctgcttt tgatattcag
    12481 ccacaagcac tggataattt taccaaacta catcagattg aaaaacaata tactaatttc
    12541 gaagagatgg taaaaaaccc aaaaatcgag ggtgtttaca tcggaacacc gaatcaaaca
    12601 cactatcctt tgatgattca agctcttcaa gcagggaaac atattatctg tgagaaggta
    12661 atgactttga atagtgctca attagcacat gttcaatccg ttgcaaaaga aaaacagttg
    12721 gtagttattg aaggaattac acttttttat atgccgctat tttccaaaat taaacaaatg
    12781 attgaattag gaatgcttgg aaaaataagt ggagtcaatg tgacttttgg tagtatgaag
    12841 gaatttaacg ctactaatcg cttttttgat cgtaaaaaag gtggtggagc attatttgat
    12901 atcggaacat atgctctttc agctgtagta tatttcttag gaacacaaac aaaactgtta
    12961 aatacagaag tagtgatgtc tgaaactgga gtagatgaaa aatctgtttt ttcgctaatt
    13021 aatgaagatg gaatccttgc ctcagcaata atttctttta gaggaaaaat gcctaaacag
    13081 atcattatta gtggagaaaa agcttatatt acaatcaatg atttccctag agcagaagaa
    13141 gcggtaatct tttggaatga tgggagaatg gaagaactca aatttggcga tgagagagac
    13201 atttttaatt tcgaagttga aacagtcaat cgctatttga aaagtgattc aaacgaaatg
    13261 gatgtacgag aagtaacgaa atgtgttatt ggaataatgg atcaagcacg agaacaatgg
    13321 ggattgacgt atcctaatga agaattagtg tagaaaaatt ctttgataga tatgctctat
    13381 cggtatggaa taccctcttg atcacaactt tagaaatttc tatccataaa taaaaacgaa
    13441 tcagagaagg tgtctaaacg atgaaaagaa tactgacact tggtgaaatt atgcttcgat
    13501 tatcaacgat tgaggatagt cgcttggagg aaagtacgcg tttagccgca cattatggtg
    13561 gtggcgaggc aaatgtagcc atttcactag caaattttgg acatcaagcg ctatttgcaa
    13621 gtaaaattcc agaaaatgct ataggaaagg gtgctaagca tcatttgatg aagtatggag
    13681 tcgagacttc tcatctcttg atgggaggag agcgattagg tacctatatt tagaaaatgg
    13741 cacgggaatt cgttccacct ctgtggttta tgatcgatgt cattccagtt tttcaaaaat
    13801 ggatgaatcg gaatgggatt tagatcagtt gtttgaaaat gtacatcttt ttcatatttc
    13861 tggaattaca ccggcacttt ctttaaaatg gcaagattgg gtactacttc taatcaagga
    13921 agcaaaaaaa cgacaaatta tcgttagttt agatattaat tatggcggaa aactttggtc
    13981 agtgaatgac tgtaggagct atcttcaaag gattgttcaa tacgttgatt ggtgttctgc
    14041 ggcgcgttta gatgctttga atttttttga gatactagaa aaagaagacg ctgatcttaa
    14101 ctattattat catgaaatta ttcaacgata tgcgagtatc aaagtacttt attctacttc
    14161 acgagaagta ataacacccc aacaccatcg attacaaggg aacctattga ttgaagaaag
    14221 tctatttcaa tcaaaagtca ttgatattca aaatattgtt gaccgagttg gtggtggaga
    14281 tgcgtttgct gcaggaattt tacatggact attaacaaaa atgacaccac aaaaaacaat
    14341 cgattttgca actaccgctt ctgcattgaa acactcaata catggtgatt gtaaccagtt
    14401 cagttctgag gaagttgaac aggttttaaa cgaaagaaat ggtgatgtga agagatagtg
    14461 agccattgtc tataaagtta gatttaattt gaatcatcaa caataataaa aaagcccagc
    14521 attcttccta ttctattgga agtacgctgg gcttttaaat tcacctttac ataacggttt
    14581 aatcaatacc acgttt
//
LOCUS       DANKHK010000062        14547 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000062 DANKHK010000000
VERSION     DANKHK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            134..454
                     /locus_tag="PW109_001789"
     CDS             134..454
                     /locus_tag="PW109_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511606.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            906..1106
                     /locus_tag="PW109_001790"
     CDS             906..1106
                     /locus_tag="PW109_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2511607.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1253..4042
                     /locus_tag="PW109_001791"
     CDS             1253..4042
                     /locus_tag="PW109_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2511608.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4089..4616
                     /locus_tag="PW109_001792"
     CDS             4089..4616
                     /locus_tag="PW109_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2511609.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4637..5827
                     /locus_tag="PW109_001793"
     CDS             4637..5827
                     /locus_tag="PW109_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2511610.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5867..7165
                     /gene="asnS"
                     /locus_tag="PW109_001794"
     CDS             5867..7165
                     /gene="asnS"
                     /locus_tag="PW109_001794"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2511611.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8331..9005
                     /locus_tag="PW109_001795"
     CDS             8331..9005
                     /locus_tag="PW109_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2511612.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECVNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9002..9343
                     /locus_tag="PW109_001796"
     CDS             9002..9343
                     /locus_tag="PW109_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511613.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9373..9732
                     /locus_tag="PW109_001797"
     CDS             9373..9732
                     /locus_tag="PW109_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2511614.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10256..12337
                     /gene="cadA"
                     /locus_tag="PW109_001798"
     CDS             10256..12337
                     /gene="cadA"
                     /locus_tag="PW109_001798"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2511615.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12648..14114
                     /locus_tag="PW109_001799"
     CDS             12648..14114
                     /locus_tag="PW109_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2511616.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4817 a         2528 c         3102 g         4100 t
ORIGIN      
        1 aggttgtgac gtggtcacga cctctttttg acgattatga tacaaaaaac atcatttatg
       61 aacaaaatgt gaataacggg cgttcttgag atatgatttt aattgggaaa aacttatcga
      121 taaggaggtc tttatgaaaa aaagactgtt atggtgtttg ctgtttctat ccctaagttt
      181 aacaagtcag ccagtttttg ctgaagaaac acagcggtct cctattacag gcacacgtct
      241 gtacaaaaat gtttatgtac agacaacaga tcttgattct gcgattcctg aaacagaaga
      301 acggtctttg cagccaagat ttggttactt ttggtactgt ccgacctgtg attatgaaag
      361 ttgctggcat ttcttacatt ctacagctgc agcttttgct tttactcata ctcagaaata
      421 tgatggacat catcctgtta tttatgaggt atagatatga gcaaaaaaga aaaactaatc
      481 tcgttgatcg tattggtttg ccttgttctt ctggtaactg tcataggcta tcagaaatac
      541 catgagaaca tcagacctac taaagaatct tccccagcct cgaccgttga aacagaaccg
      601 ataagtagtc agaaagaata atttttttag tttaaagaag aacatcagag caaaaaaaca
      661 ccaactgaac aatatgtcgt ttattttaaa aaaagttcgg tttttcactt tatgtaatcg
      721 ctgagaaaac tttttttaat aaaaaaagtc gaaaaactct agcttttttg taaacggtat
      781 gatatactat aggagtagtt tttgttcggt aatctgtggt agcttgtttc aaataaacat
      841 cttccagata tacaccattc aagtaattgc aaaatatgtg ttgatacaca aggaggattt
      901 ttattatgga aacaggtaca gtaaaatggt ttaactcaga aaaaggattt ggattcatca
      961 ctgcagaaaa cggaaacgat gtattcgttc atttctcagc tatccaaggc gacggattca
     1021 aaactttaga agaaggccaa gcagtaactt tcgatgttga agaaggccaa cgcggacctc
     1081 aagcaacaaa cgttactaaa gcttaattaa cctatactaa aaaataaacg cccagacatt
     1141 tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg atgagagtat ttttctgcac
     1201 tttgtagtat aatttcaaaa gaacattaga ggcaggagaa gaggaagata ttttgaaacc
     1261 aagaacttat gcagtcgttg atatcgaaac gactgggacg aatccaaaag aagatcggat
     1321 catccaattt ggctgcgtca tgattgaatc aggtcggatc acttcacgtt tttcgatcga
     1381 tattcatcca gggcggaaga tttcaaaaca aatccagcat ttaacaggga tcacgaatca
     1441 acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa acgatttaca atcttttggc
     1501 agacaccata tttgtcgcgc ataatattta ttttgattat aattttttga accatgaact
     1561 catgcgatgc ggacttccta gtttgaagat accaggcatc gatacagtag aacttgcaca
     1621 aattttcttg ccgacggaac caagctttcg cttagctgat ttgtcagaaa gccttggatt
     1681 tattcatgag aatcctcatc aggcagatag tgatgccgaa gtgacgggtc aacttctttt
     1741 gctgatcgaa gaacggatga gaaaattgcc aatcatcacg ttagagcaga ttgcgcgatt
     1801 aagtagacac acaggtatgg atacgagtcg tttcatttat catgtgattg aagaaatgaa
     1861 agaaaaacca gagccacttg atccctcatt agaaatcgtc gatgggctag ctttgcagaa
     1921 aaaagaagtg gaactattca catccgtcca ttatggcgag cggacatatc caagaaaaaa
     1981 acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat cgaaaagaac agaacagact
     2041 gatgaatgcg gtctacgacc atttcacaaa agatgaatca aaagatttga tgatcgaagc
     2101 agctacagga atgggtaaaa caatcggtta tctgctgcca ttgagttatt tggctacacc
     2161 tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg cagcagcaga tcatagaaaa
     2221 agacattcca ttgctgaatc aattgctaga tcagcccata caacctgtaa ttatcaaaag
     2281 ctatcgccac tatattgatt tacagcgttt taaaggcaca cttgtggaac cgccagaaca
     2341 aaaacaatat gctttgtatc aaatggcggt cttagtttgg ctgacgcaaa cgaagaccgg
     2401 tgatttggat gaattacatt taacaaattt gaaccattca ttctttgcag atatcgcgca
     2461 cagaggtatt ggatttttag caagaaatca gtcattttac gaacaagact ttgttcgttt
     2521 cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc aatcatgcgt ttttaataca
     2581 agaaacacag cgaaaagaac cattgcttcc agctagtcca tttttgttga tcgatgaagt
     2641 acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa cgattaagta ttaactattt
     2701 ccataaacaa atccagcaat tagaagaacc aaatcagctg tttgaccgaa tacaaagcta
     2761 tttaccgaaa gaacatgaag ccttacgctg gttatcgatt tatcgagaag aactttctgc
     2821 gctatgcgaa gcacagattt gtatggcaga aggattaatg agtttagtga gcggcaagca
     2881 acctaacgga cagtatccag aagaagtcat gatgacaaaa gagatgttgg atcagctgcc
     2941 actggaatct gggacagcca ttgatcacct gcagcttttt tatcaggaga tccagcagct
     3001 acagtcacaa atgcgtctga cttttgagat tatccaagaa agctggacaa accagatgcg
     3061 gatcgattta gcaagtttat atagcctatt tgaccaatta gacaagcagt acttgctgat
     3121 ggatcaatgg ctggaagact ggcgagatca tttgatccat tggttcgttc ctgggaacac
     3181 agcgcagcag atgatttttc atctccatga ttttcaagcg gctatgttgc cgtcgactgt
     3241 ttggtatcct cgatatgatc gtattcttta tattggagga acgctgaaga tcggccctaa
     3301 tcgacactat ctctctaaga ggcttggcat tgaagcagca cctcttaaag tgattgcctc
     3361 tccttatgat tatgagaaac aggcaaggat ctatgtgcca aaagaggcac ctgccgtcca
     3421 tcaagtcagc aatcaagcat atatcgatta tttagagaca gcacttacag gtctgatcga
     3481 acaagaagat cggccaatac ttgtgttatt cacttctcat gatgttttgc agaaggtcta
     3541 tcagaaaatg catctgtcta tgctagaaaa aggacgagaa atactagctc aagggtttgg
     3601 cggaagccgg gagaagcttt tgaaacgctt cattttgtca gaaaaaagcg tattgtttgg
     3661 cgctgacagc ttttgggaag gtgtagattt gcctggagat gctttgcaga tcgttgtagt
     3721 cacacgttta cccttcgata atccgcttcg cccgatggtc aaagctagga acgcttattt
     3781 agaatcagag gggatcaatc ctttttacca ggaatccgta cctaaagcag ctttgaagtt
     3841 acgccaagca ttaggtcgct tgataaggac ggagacagat cgtggcgtat tgcttatttt
     3901 ggatcgaaga ctagtcactg caaaatatgg tgcacggatc atccatgcat taccgaaaaa
     3961 agtaccgatc aaagagctag ctatggagga aatctatcag gatatccatg aattcttaaa
     4021 gaaaaatgaa gaaggaagtt agaggagctg tcttttttca taattaaaat ctgctataat
     4081 gaaaaggaat gaagataaag gagcgacgac ccttgaagaa aaaacagaca aaggaaaaaa
     4141 cgattaatcg cgtgttactt ggcttgatcg tatttttatt agcggtcatc gtgatcagct
     4201 cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc gaagaaagaa gcagtagaaa
     4261 tcgcaaagaa atatgcaaat cttgaggaag ccgatcattt ttattggttt actcgaaaga
     4321 agacatactt ttcactgaca ggtacaaatg ataaaggaaa agaaatcgcc gttatcattc
     4381 ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa gggactgact gaaaatgaag
     4441 caaaacagac aattgctgcg gcccatcctg aaatccaaat cgaaaaagct gctttaggga
     4501 tgtatgatga tcagcctgtg tgggaagtag taggaaaaga aacaaatggg aaactgaatt
     4561 attacctgat tggctttgaa tctggagatg aagtcaagac aatcaaggga atttaaaagg
     4621 taaaggaatg aatgggatgg agttatcaaa aagaacaaaa cgattagaac cgtctgtcac
     4681 tttggcagct gcggcaaagg ccaaggcatt aaaagccaaa ggcagagatg tgctgagttt
     4741 gactgtggga gaaccggatt ttgcgacacc agaaaacatc caagaagcag caatcgaagc
     4801 aatccgcaat gggaaagcta gctattatac accaacagca ggaatcccag agcttcgaca
     4861 ggcaatcgtt gattatctga agaaaaacta tggacttgtc tatgaacctt cacaaacagt
     4921 cgtaacagac ggagcgaaat ttgctttgta tacccttttc caaacgatcc ttgacccgca
     4981 agatgaagtc atcattcctg tgccttactg ggtcagttac ggtgagcaag taaaactggc
     5041 agaaggactt cctgtctttg tgaaagggga agaatccaat agctggaaag ttactgttga
     5101 gcagctagaa caagcacgtt cagaaaaaac aaaagccgta atcatcaatt ctccatcgaa
     5161 tccgactgga atgatttata gcaagaatga actgcaggca atcggtgaat gggcagtaaa
     5221 aaatgatatt ttgatagttg ctgatgatat ttatggccgt ttagtttata atggcaatga
     5281 gtttactcca attgcaacga tttcagaagc aatcaaaaat cagaccatca tcatcaacgg
     5341 cgtgtctaaa acatatgcga tgacaggctg gcgcattggt tatgcggtgg gaaatcctgt
     5401 gatcatcaat ggaatgatcg ctgttgcttc ccaatcaact agcaatccga cagcagtcag
     5461 tcaatatgca gctgttgaag cattgaaagg cgagcaagat acagtggaag aaatgcggaa
     5521 cgcctttgaa gaacgcctga atacgttata tccgcttgtg gcagaactgc cgggtgtttc
     5581 tcttgaaaag ccacaaggag ctttttatct ttttcctaat gtaaaagaaa cgctacgcat
     5641 gtgtaaatat gaaaatgtca cacaatgggt agaagatctt ttagaagaaa caggggtagc
     5701 gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc cgcatgagtt atgccactga
     5761 tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa tttattgaga gtaaaagtca
     5821 aaattaatcc tgaagaaatt caaggaatat aaaaggagag tcatacgtgg aacaaattca
     5881 aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca atcggcgctt gggtcgcaaa
     5941 taagcgatca agcggaaaaa tcgcattttt acaattaaga gacggaacag cctttttcca
     6001 aggtgtagtt gtaaaaagcg aagtgccgga agaagttttc caaacagcta aaaatctgca
     6061 ccaagaaaca tccgtgttag taactggaga aatccgtgaa gacagccgtt caaaatttgg
     6121 ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag agccacgatt atccgatcac
     6181 accaaaagaa cacggaacag acttcttgat ggatcatcgt catttatggc tacgttcttc
     6241 aagacaacat gcgattatgc agatcagaaa tgaaatcatt cgtgcaacgt atgaattctt
     6301 taatcaacat cattttgtca aaatcgatcc gccgatcttg actggttccg caccagaagg
     6361 cactacggat ctattcgaaa caaattactt tgatcaaaaa gcttatttat ctcaaagtgg
     6421 ccaattatac atggaagctg ctgctatggc tttcggaaaa gtcttttcat tcggtccgac
     6481 tttccgtgct gaaaaatcaa aaacacgacg tcatttgatt gaattctgga tgatcgaacc
     6541 agaaatggct ttcactcatc aagaagaaag tttagaagta caggaacaat atgtagcatt
     6601 cttagtacaa aatgttttag atcattgcga atacgcatta gatgtactag gacgagacaa
     6661 agaagtctta aaaacttata caaaattgcc attccctcgt attacttatg atgatgcgat
     6721 cgaattactg aagaaaaatg gatttgatga catcgaatgg ggcgaagact ttggttcacc
     6781 tcatgaaacg ttcattgcga attcattcga acagcctgtc tttatcctaa actatccaaa
     6841 agctatcaag ccattctaca tgaaaccaca tccaacaaga gaagacgtgg taatttgtgc
     6901 agatatgatc gcaccagaag gttacggtga aattattggc ggaagcgaac gggcaacgga
     6961 ctatgatttc ttgctagaac agatccgtaa tcacggtttg gatgaaaaag aatattcatg
     7021 gtatcttgat ctacgtaaat acggttctgt tccgcactct ggttttggtc taggccttga
     7081 acgaacagtc acttggattg ctggtatcga ccatgtgcgt gaagctattc cattcccacg
     7141 tttgttgaac cgaatttatc cataagaggt gatgaaaaag ctgttctgca tcaagtaatt
     7201 agaacagcca agcaaaaaca gctgttcatg tttttgaatt ggcgggactt attaccgcag
     7261 atgtaagctt ttgaataccg tttagtggag aggttgtgaa aaagctgttt agtttcgagt
     7321 attaagaaaa attttgtgga aatggctttc cacatttttg cataaatttg gcttaatacc
     7381 gagaaactgg cttttgaaca ccgtttaagg aagaggttgt gaaatcagtg ccttgacctg
     7441 agtattagag aaacaaaacg gaaaaatagc ttctcatatt ttaccgtttt tttagctaat
     7501 atcgagggtc agctgattga acaccgttta tatcaagagg ttgtgcgcct gccgttcagc
     7561 tccgagtcac aaggagtaat ttcaaaaaac agtttttcat gtttgatgac tattgtgact
     7621 tgtggccgga gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt
     7681 ttaaaatacg ggataatgga agtttccagc agacaagaga agaaagcaaa agaaattgct
     7741 atttgattca caaggttcta taacgaaatc gcaatcaatc aaacatttac aaagaaggta
     7801 attcaaatgg aaatctgatt tgatgatgct tctgctgttg aaataaagca gcatctagca
     7861 cctgaaaaca aactaatagt aacattcgaa gatggagtag gtccgtattt tcagcatgca
     7921 ataatccata tgtaagttca attcaccctt gaaatcatcg gaccagataa agttatgacc
     7981 aatctatccc aagcaatcta gaagagatat tagtaaaggg acatttacta gaagtcttag
     8041 atgagcctat aaatatatgt tttgatggcg tcatcagtgg tctgattgac ggaagcttgg
     8101 gtatgatcga tctctctgga ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag
     8161 aaaaaaatag cataaaaaac tagacacttg atgagcgttc attcaattgt ttgaagggac
     8221 taggctggtg tcatttttta tacatatatt ttctctttca tcttgatagg gcttatggga
     8281 taatataagg aagaaaatca tcgttttatt caaaaaggag tttttgacaa atgaaaaaac
     8341 gtatattatt tatcggtgat agcattacag acgcaggaag agaccgagca gaagcaactg
     8401 atctaggtaa aggttatcca cttttgattg ccggaaaact aatctcccaa tatccaacga
     8461 tggaatttga atgcgtgaac cgtgggatag gaggagacaa aattgcagat ctgcaagtac
     8521 gctggcaaga agattgttta gctttacaac ctgactatgt atcgattttg atcggaatca
     8581 atgatacctg gcataatact agcgatcagg ctatattcgg aactgagaaa tctgctcagc
     8641 aatttgaatc cgtttatcga aaattactaa atgaacttca aaaagcggga atctgcaaca
     8701 tcatcttgat ggagccgttt gtacttccta taaccgacga ccgtcatggc tggcgtaaag
     8761 acttagaccc taaaatccaa attgttcgta aacttgctaa ggaatatggc gcattgctgg
     8821 ttcctttaga tggtatattc aatgctttag ggattgctta tggtttttcg aaatatacaa
     8881 gagaagatgg ggtccaccca acgattggcg gacatgagat cattgcacaa agctggatag
     8941 atgcaattgc cgcagtcgat ttttttagtg gagataaaaa tgagcgaaag aaaagtgaaa
     9001 aatgatggaa ataaggtggg cagtaaaaga agatttacct caaatcaatc agctgattac
     9061 tgatcatcca gaatcattcg taaaagcaaa aacggcagct gacctaaatg ataagaataa
     9121 actattgctc attgctgtaa caaaagaaaa acagattatc ggatttgcgc aagcggacgt
     9181 gcaacagtca gaagaacgat tggatcagcg accatcagga aaactaatcc aacacacatt
     9241 agtaacaaaa gaaaatacga aagaaatcca aaaaaaattt gcagaatact tgatgctgga
     9301 agctaaaaaa agaagaataa aatcaatttt tgcccagttt taatcaaaaa cagtaataga
     9361 aggagaaata caatgagttg tttaggaaat gtcatctggt ttatattcgg cggactaatc
     9421 ggtggtctta gctggttagc agcggggata ctttggtgta tcacgatcat aggtatccca
     9481 atcggtatgc aatgttttaa gatcgcctcc ctttcttttg cgccttttgg caaagaagta
     9541 atttatcacc agtctggcat gtcattgatc gttaatatca tctggctgat cgtttcaggc
     9601 ctaccaattg ctctcgcaca tgtcgtcagc ggcatccttc tatgtatcac gatcataggg
     9661 ataccttttg ccaagcaatc tttcaaactt gctcgattgg cgttgatgcc attcggtgca
     9721 gaaattgtat aaaagagaat aataaaggtt agataacgca tagagtcagg tgggatacat
     9781 ttatttcacc tgactctatg cgttttcctg tataagttct tccatgtgac ttttacgtct
     9841 tttatttata tgggattact gtttatggaa agttgaaata attttcatta aagaactctt
     9901 tctatatttt tgaaagcgta gtggagcttc gaatatctgg gaaggacatt ttgcagggat
     9961 tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta gacaaattgc cagcaacagg
    10021 cttcaaactt agttgctatc ctgtcaagat caagaaggca agtgcgggtt ggataagagc
    10081 agtggcaatg atcgaaaaga aaaagaaaga ataagccaaa acatctgtat ggaagaaatt
    10141 ttccgatgca gatgtttttc attttttctt tcggatacct tgacatatga ataaccattc
    10201 atttataatt agttatgaag agatattcat atgttgaggc gaggaggtca ttaaaatgaa
    10261 aagttacaga ttagaaggtt tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt
    10321 tcagaaaatc aatggagtca aggaagcaac ggtaaacttc acttctggaa aactaacgat
    10381 tgacgcagag gaagatcatt tggctacgat tgaacaagaa acaaaaaaag tagtgaagga
    10441 attagaacca gatgtcaaag taacagaaat agataaggaa aaagtatctg aacatggaaa
    10501 tgaaaaagag agaaatacgc tgttccggat tttattttcc cttgcaggta tcgctttatt
    10561 gctattattt gattttaatg aaccaattcg cttgataggg tatttattga tttatctgct
    10621 gataggatat gatgtagtga agaaggcagt gatgaatatc gtcaaaggaa aaatctttga
    10681 tgagaatttt cttatgtctg ttgctacgat tggcgctatg ttgatcggtg aatatccaga
    10741 agccgtagca gtgatgctat tttatcaaat cggcgagtat ttccaaggat tggcagtcag
    10801 tcattcaaga aaatcgattc gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca
    10861 gacggcggaa ggattgatga ccgtgaaccc tgaagacgtg cagatcggtc agtttgtact
    10921 tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc atcgaaggag aatcattagt
    10981 cgatacgtca gcacttacag gagaatcagt ccctaaatct gtatatgttg gtgaaacagt
    11041 attaagcgga tttatcaata aaaataaacc atttttagtt caagtagaaa aatcttatga
    11101 aaattctact atcagcaaat tattagaatt agtagaaaat gccagcagta aaaaagcacc
    11161 agctgaaaat ttcattacta aattcgcgcg ctattacaca ccagtcgttg taggattggc
    11221 cgttttatta gctgttttac ctccacttgt tgtttctgga gcggcgtttt ctgaatggat
    11281 ctatcgagca ttgacatttt tagtgatctc atgtccatgt gcattagtga tttccgtgcc
    11341 acttagtttc tttggcggta tcggaggagc aagtaaaatc ggtgtattag taaaaggttc
    11401 aaattacttg gagttgcttg cacaaactga gacagtggta ttcgataaaa ccggcacatt
    11461 aacgaaaggt gacttttcga tccagacgat cgatacaaaa atcgatccaa agatattcat
    11521 gcaatatgtt gcgagtgcag aacagttttc tacacatccg attgctcaat ctgttttaga
    11581 aggctacgca ggtccgcttt taccaacaac taatatccaa gaatttgcag gtgaagggat
    11641 cttagctgaa gttgatggca aacaagtatt agtaggtaat cacaaattga tggaaagatt
    11701 tgaaatctct tttccttcaa gtcaagaaat cggtacattg ctttatctag cgatcgatca
    11761 aagttacagt ggttatcttg ttattgcaga taccttgaaa gaagatgccg tagacgcatt
    11821 ggttcaatta aaacaggctg gtgtgaagaa cacagtcatg ttgacgggcg actcgaaaaa
    11881 aattgctgat catatcggga agcaagtagg cgtggacaag atatatagcg aattgcttcc
    11941 agaagataaa gtgcagcggt tagaggaaat cttacaaaga aacaataaaa agaccgcatt
    12001 tgtcggagat ggaatcaacg atgcaccggt gctagcaaga gcagatgtag gaatcgcaat
    12061 gggtggattg ggaagcgatg ctgcaattga agcagcagat gtagtcatca tgaatgatca
    12121 accatcgaag attgcagaag caatccattt agcaaagaaa acattgaaaa tcgtcaaaca
    12181 gaacattgtc tttgctattg ggataaaaat tttggtactt gccttaggtg cttttggttt
    12241 tgcatcaatg ggagatgcag tatttgcaga tgttggggtg acagtattag ctgttttgaa
    12301 tgccatgcga agcttgcatg taaaaaataa acgttgatca atgaagagga cgaatgaacg
    12361 tcctcttttt tttatatcct tttcttaatg aaaaatgtaa agaaaataga taagattata
    12421 aaataactaa tgtttttaat tttgcagaaa attcaaaata gcttgaaatt caataaggtc
    12481 aatgaaataa aagaaaataa cttatttttt tgatggaaaa aatatgaaaa aacgatgtgt
    12541 attaaataaa agaatacaca cataaaataa cactgtcttg taagtttgtt aaaaattgaa
    12601 tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg aacaacaaac
    12661 aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc atggggaatg
    12721 tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta ttgattttgg
    12781 ccatttattt tattccatac gcattgatcg ttggacaatt aggatcagcc ttcaaggata
    12841 gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga ctggcatact
    12901 atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag cctcaagcaa
    12961 tcctgattgc tggcggttgg gcagtccaag gaaatggaaa tatcgtaaat acgatgtcag
    13021 tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat ttatcaacaa
    13081 aaggactttc tactttgaaa gtgattggtg gactggccgg aacagcgatg ttcgtcatgt
    13141 cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg atggaatttg
    13201 ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc agttatttca
    13261 caaccgtttc gatgctagta tttgcagttg gaggagcgga aaaaatatcc ccttatgtca
    13321 actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta gcagcaatgg
    13381 tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct agcgataata
    13441 ttcctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta ggtaattact
    13501 atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt ggacaaatct
    13561 ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat gcggatccag
    13621 aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg aatggttatc
    13681 ttctgacagg tatactagta ggaatcatta ttttgttgcc gatctttggt atcaaagata
    13741 tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca atgcgttacc
    13801 tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc aattctgaat
    13861 ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc tttgccttca
    13921 ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct gatcctaaag
    13981 catggtggtt ccaattattc acgaatatca tcacaccaat cgtcttgatc ttattaggta
    14041 tgatcttgcc agcaattgct cgtcgagaca aaaacaaaga aatcactgaa ccagaaacag
    14101 tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgacagaag gggtctcagc
    14161 ttcaagaaat aagaaggaat gtacgaaaat tgcttctcaa atttttgtga agtttcggct
    14221 tgtttttcga agaagctact tctggaacac cgtgaatcag aggttgtgaa aaagctgtcc
    14281 tgcatcaagt aataagaaca gccaaacaaa aatagcccct catatttttc gtttggctgg
    14341 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg taaaatcagc
    14401 gatttgacct gagtattaga gaaacaaacg gaaaaatagc tcctcatatt ttgccgcttt
    14461 ttgagctaat gctgaaggtc agctgattga acaccattta ttcgaaacaa gggactttga
    14521 cacgactttt gtcacagtcc cttgttt
//
LOCUS       DANKHK010000063        14312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000063 DANKHK010000000
VERSION     DANKHK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(359..1627)
                     /locus_tag="PW109_001800"
     CDS             complement(359..1627)
                     /locus_tag="PW109_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2511617.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1858..2595)
                     /locus_tag="PW109_001801"
     CDS             complement(1858..2595)
                     /locus_tag="PW109_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2511618.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2662..3231)
                     /locus_tag="PW109_001802"
     CDS             complement(2662..3231)
                     /locus_tag="PW109_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2511619.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3359..4231
                     /locus_tag="PW109_001803"
     CDS             3359..4231
                     /locus_tag="PW109_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2511620.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4573..5445
                     /locus_tag="PW109_001804"
     CDS             4573..5445
                     /locus_tag="PW109_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2511621.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5442..7400
                     /locus_tag="PW109_001805"
     CDS             5442..7400
                     /locus_tag="PW109_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2511622.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7732..8583)
                     /locus_tag="PW109_001806"
     CDS             complement(7732..8583)
                     /locus_tag="PW109_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2511623.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8706..9173
                     /locus_tag="PW109_001807"
     CDS             8706..9173
                     /locus_tag="PW109_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2511624.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9436..9918)
                     /locus_tag="PW109_001808"
     CDS             complement(9436..9918)
                     /locus_tag="PW109_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2511625.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(10018..10881)
                     /locus_tag="PW109_001809"
     CDS             complement(10018..10881)
                     /locus_tag="PW109_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2511626.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            11479..12354
                     /locus_tag="PW109_001810"
     CDS             11479..12354
                     /locus_tag="PW109_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511627.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(12499..12651)
                     /locus_tag="PW109_001811"
                     /pseudo
     CDS             complement(12499..12651)
                     /locus_tag="PW109_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(12919..14250)
                     /locus_tag="PW109_001812"
     CDS             complement(12919..14250)
                     /locus_tag="PW109_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2511628.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         4377 a         2849 c         2533 g         4553 t
ORIGIN      
        1 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
       61 ttattgattc ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc
      121 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
      181 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
      241 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
      301 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
      361 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
      421 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
      481 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
      541 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
      601 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
      661 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
      721 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
      781 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
      841 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
      901 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
      961 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
     1021 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
     1081 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
     1141 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
     1201 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
     1261 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
     1321 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
     1381 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
     1441 aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc
     1501 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
     1561 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
     1621 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
     1681 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc
     1741 tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
     1801 tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta
     1861 tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc
     1921 tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc
     1981 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
     2041 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
     2101 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
     2161 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
     2221 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
     2281 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
     2341 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
     2401 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
     2461 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
     2521 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
     2581 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
     2641 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
     2701 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
     2761 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
     2821 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
     2881 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
     2941 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
     3001 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
     3061 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
     3121 cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc
     3181 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
     3241 tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact
     3301 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
     3361 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
     3421 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
     3481 agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg
     3541 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
     3601 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
     3661 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
     3721 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
     3781 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
     3841 ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat
     3901 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
     3961 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
     4021 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
     4081 tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca
     4141 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
     4201 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
     4261 tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt
     4321 tgttccttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
     4381 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
     4441 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
     4501 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
     4561 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
     4621 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
     4681 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
     4741 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
     4801 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
     4861 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
     4921 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
     4981 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
     5041 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
     5101 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
     5161 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
     5221 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
     5281 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
     5341 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
     5401 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
     5461 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
     5521 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
     5581 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
     5641 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
     5701 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
     5761 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
     5821 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
     5881 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
     5941 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
     6001 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
     6061 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
     6121 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
     6181 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
     6241 aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg
     6301 gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc
     6361 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
     6421 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
     6481 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
     6541 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
     6601 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
     6661 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
     6721 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
     6781 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
     6841 tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa
     6901 acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta
     6961 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
     7021 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
     7081 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
     7141 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
     7201 tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
     7261 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
     7321 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
     7381 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
     7441 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
     7501 ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga
     7561 ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa
     7621 aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa
     7681 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
     7741 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
     7801 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
     7861 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
     7921 acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc
     7981 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
     8041 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
     8101 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
     8161 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
     8221 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
     8281 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
     8341 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
     8401 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
     8461 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
     8521 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
     8581 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
     8641 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
     8701 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
     8761 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
     8821 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
     8881 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
     8941 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
     9001 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
     9061 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
     9121 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
     9181 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
     9241 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
     9301 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
     9361 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
     9421 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
     9481 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
     9541 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
     9601 cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt
     9661 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
     9721 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
     9781 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
     9841 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
     9901 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
     9961 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    10021 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    10081 ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc
    10141 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    10201 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    10261 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc
    10321 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    10381 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    10441 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    10501 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    10561 tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc
    10621 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    10681 tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    10741 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    10801 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    10861 ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc
    10921 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    10981 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    11041 atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag
    11101 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    11161 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    11221 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    11281 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    11341 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    11401 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    11461 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    11521 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    11581 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    11641 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    11701 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    11761 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    11821 cttatagaac atccgcaatt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    11881 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    11941 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    12001 gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    12061 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    12121 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    12181 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    12241 tccataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    12301 atagataaaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    12361 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    12421 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    12481 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg ggtagatttt
    12541 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc
    12601 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc actctaatct
    12661 ttaaagataa atattaaaat aacgattaat aatattaaca gtctttattt ttcaccacca
    12721 aaaacctatc tttgcatata ctaaatagac gtatatacac agataggttt gtcttgtgaa
    12781 aaataatgat tatccttacc aaaaaatgtt tgtttttcta catgttcgta cttatcaaaa
    12841 aatatgatcc ttattgaaat tactggctat ttatattcca acgacttctt gtgttaattt
    12901 ttgttatctc atagtaaatt atgtttcatt ttcacttaat ttagttgtca cgtgttcgtc
    12961 aattcgtaaa aacggtaaat atataaacac acttgcggct ataattaata tttgtaaaac
    13021 agctgctcgc cagtctccgg ctgttgctaa aaatcccgat ataactggtg gcgtagtcca
    13081 aggaatttgc actactacat gattaattaa tcccacagca gttgcaaaat atgctactgt
    13141 caaagaaaaa atcggtgcaa gcacaaaggg aataatcaga ctgatattaa atacaatagg
    13201 caatccaaaa ataataggtt cgcttatatt gaatatagcg ggagttaccg ataatttagc
    13261 aaagtcctta taatcttttc tacgactgaa gataaatatt gcaatcaata aagcaatcgt
    13321 atttcccgat ccacccatta ccgcaaaggt atctttaaag gccatattga taatatgtgg
    13381 aatttgctct ttgtttgcat aagctgccat attttcttgc atattctgaa tcaaaaacgg
    13441 atctaacaat gaacctgata ttactgcttg atggatacca aatccaaaaa atagattagc
    13501 aatagatgta attaacaaaa atcctggtaa accagttgta attttactta atggctctgt
    13561 aactatcgtg gtaattagct tatttatatc catattaaac aattgaatga ttataaatga
    13621 caataaagca aacgaggtaa tcgtaataat gattgggatt aatacattaa aagactttaa
    13681 aacagcggga ggaatatttc cggatagctg aatctgtagt ttcttattat tagaaagttt
    13741 aataaataat tctgtagccg ctaatccaac aaatatacct acaaacattc ctgaagctcc
    13801 tgtataggat ataggtatca cacccgtcac ttcaacagct ttatccgcat tttcaggagt
    13861 aaacatcatc tgcataggtg ttaatacaac aaatgtcgaa atagcaacta ataccgctgc
    13921 aatatgattt gtataatttc tgcttgcaca catatgataa gatactgctg ctacaactaa
    13981 taatgtagtt atgcttaatg taccattggc aattgaagta ccaatttgtt gtaacgtggt
    14041 caatgtacta gtatcgacaa tttcccccat gaatcctgtt ggttctaaga tcactgaatt
    14101 aataagtgtc acaaatcctg ctaacatcat aaatggcata aatgtagcaa acgcatctcg
    14161 caaagttctt aaatggatct gattccccaa cttagtcgca actataccaa atttttcaaa
    14221 tacaatgtcc ttcctgctac ttttgcccat attttttctc ctttatgctt attgtaatcg
    14281 attacttttt ccacccaaat aatacatcac tt
//
LOCUS       DANKHK010000064        14096 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000064 DANKHK010000000
VERSION     DANKHK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(8..3682)
                     /gene="rpoC"
                     /locus_tag="PW109_001813"
     CDS             complement(8..3682)
                     /gene="rpoC"
                     /locus_tag="PW109_001813"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2511629.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3740..7357)
                     /gene="rpoB"
                     /locus_tag="PW109_001814"
     CDS             complement(3740..7357)
                     /gene="rpoB"
                     /locus_tag="PW109_001814"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2511630.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8085..9053
                     /locus_tag="PW109_001815"
     CDS             8085..9053
                     /locus_tag="PW109_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2511631.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9071..9976
                     /locus_tag="PW109_001816"
     CDS             9071..9976
                     /locus_tag="PW109_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511632.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9973..10779
                     /locus_tag="PW109_001817"
     CDS             9973..10779
                     /locus_tag="PW109_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511633.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10915..11904
                     /locus_tag="PW109_001818"
     CDS             10915..11904
                     /locus_tag="PW109_001818"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2511634.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12221..12745)
                     /locus_tag="PW109_001819"
     CDS             complement(12221..12745)
                     /locus_tag="PW109_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2511635.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13032..>14096
                     /gene="scm"
                     /locus_tag="PW109_001820"
     CDS             13032..>14096
                     /gene="scm"
                     /locus_tag="PW109_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2511636.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3989 a         3041 c         2786 g         4280 t
ORIGIN      
        1 aaaatatcta ttttgtttga ttgttcattg cgtcttctgc agccatttgc gcttcaatat
       61 ctgaaatact gtagacattt tcgcttgcta caccgacttc ttttggttcc atgtcacgat
      121 aacgttgcat accagtacca gctgggatga tcttacctat aataacattt tctttcaaac
      181 caagaagagg atctttctta ccgcggatcg cagcatccgt caagacacga gttgtttctt
      241 ggaatgatgc agcagataag aaactatttg tttctaatga tgcttttgtg atacctagta
      301 agacaggtcg agaagttgca ggaacgcctc ctgagactag cgtattgtag ttttgatctt
      361 taaagtctgc gatgtctaat aatgtacctg gtaagatatc tgtatcacct ggatccatta
      421 cacgaacttt acgcagcatt tgacgaacca ttacttcgat atgcttgtcg ccgatttcta
      481 ccccttgcat acggtaaaca cgttgtactt cacgaagcaa gtagttttca acagataata
      541 catcgcgaac ttgcaacaat tgttttggat cgattgatcc ttctgtcaat ggtgcaccac
      601 ggtggatcat atcgccttct gctactttca tacgtgctgt ataaggaaca gtatacgtac
      661 gtgtatctgt ttttcctttg attgttactt ctttggtacg tgttgcagga tcttctgaga
      721 tatcgatgac atcccctgtt acttctgtga tcactgcttg ccctttcgga ttacgtgctt
      781 caaagatttc ttggacacga ggaagacctt gagtgatatc gtctccggca accccacctg
      841 tatggaatgt acgcatcgtc aactgagtac caggttcacc gattgattga gcggcgatcg
      901 taccaactgc ttctccgact tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat
      961 gtttgcagac accatgtttt gtattacatg tgaagacaga acggattgtc actgtttcga
     1021 cacctgcatc aacgatttgt tttgcgatgt cttcggaaat caattggttt cctgcaatga
     1081 tcaattcatt tgtttcagga tgacgtactt ctttacgggt gtaacgtccc actaaacgat
     1141 cttctaatga ttcgatgatt tcatttccct cgcggatggc ttggatttca agacctcggt
     1201 cagtaccgca atcttcttca cggatgatta catcttgggc cacgtcaacc agacgacgag
     1261 tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt catcccttta cgagccccgt
     1321 gagttgagat aaacatttcc aagacggata gtccttcacg gaagttcgaa atgattggca
     1381 attccatgat tcgaccatta ggagcggcca tcaaaccacg cattccggca agctgtgtaa
     1441 agttggaaat gttaccacga gctccagaat cagacatcat gaagattgga tttttcgctt
     1501 ccaaaccttc catcagtttt tgttggatgc tgtcttttgc agcattccaa acagcaatga
     1561 cacgttcata acgttcgtca tctgtgatca gaccacgacg gaattgtttt gtaatagttt
     1621 caacttgttt atgggcattt tcaatgattt cttgtttttc attcagtaca ctgatgtcag
     1681 cgatccctac tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt
     1741 caagcatctt agaagtttct gttactttga atcgtttgaa gacttctgcg atgatgtttc
     1801 ctaagttttt cttcttgaac ggtaatacta attcttgctc tttgatatgt gcaggaatat
     1861 cagtaccagc ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg aagtctgttg
     1921 gttcattcag ataagggaat tcaggaggca tgatctcgtt gaagatgatc ttaccaactg
     1981 ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg tttttcacct aatgagttag
     2041 ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg ataacggctt
     2101 cgttttgatc tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt
     2161 agtagttacc tagaaccata tcttgagaag gagtaacaac tggtttacca tctttagggt
     2221 tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct tcttcattca
     2281 atggaacgtg gacagccatt tggtctccat cgaaatcggc attataagct tcacatacaa
     2341 gtggatgcaa acggattgcg cgtccttgta ctaaaactgg ttcgaatgct tggataccaa
     2401 gtcggtgaag cgtaggtgcc cggttcagca atactgggtg ttccttgatt acgtcttcta
     2461 aaacatccca tacttcgtct tcttgacgtt cgattttacg tttcgcattt ttgatgtttg
     2521 aagctagttc gcgttgaaca agctcacgca tcacgaatgg tttgaataat tcaatggcca
     2581 tttcttttgg aagtccacat tggtacatct tcaagaaagg tccaacaacg ataacggaac
     2641 gacctgagta gtcgacacgt ttacctagca agttttgacg gaaacgacct tgtttccctt
     2701 tcaacatatg agaaagagat ttcaatgggc ggttacctgg tccagtaact ggacgtccgc
     2761 gacgaccgtt atcgatcaat gcatctactg cttcttgcag catacgtttt tcattttgaa
     2821 cgatgattcc aggtgcattc aagtctaaca aacgtttcaa acggttatta cggttgatta
     2881 cacgacggta aagatcattc aagtcacttg ttgcaaaacg tccaccttct aattgaacca
     2941 ttgggcgtaa atctggtgga ataacaggga taacgtccat aaccatccag cttggttgat
     3001 ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg gattgcacgt gtacgttttt
     3061 gtccttgtgc agatttcaat tcttctttca attcggctgc ttctgcatct agatcaacat
     3121 tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc ttggaattct tgtccatatt
     3181 gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg ttttttctct aaattcgtat
     3241 ttcctggttc gatgactaca tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc
     3301 tcatgtctaa tacaagaccc atacgtgaag gaatcccttt aaagtaccag atatgtgaaa
     3361 caggagctgc taattcaata tgtcccatgc gttcacggcg aactttcgaa cgagtaactt
     3421 ccactccgca tcgatcacaa acgattcctt tataacggat acgtttgtat tttccgcagg
     3481 cacattccca gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct tcgcgttcag
     3541 gttttaaggt acgatagtta atcgtctcag gtttttttac ttcaccgtaa gaccagcttc
     3601 tgattttttc gggagaagcc aaacctattt gcatactttc gaatttattt acatcgatca
     3661 aaaggggttc cctccatttc atttaatggc aaaccgcaag acggctgatc ttcgaccgcc
     3721 ttgcagctca tagaccggat tagtctaaat tatcttctgc ggtttcaaag ttttggatga
     3781 catcgtcttt ttcatcttcc acttgttcag cagctttctt ttctaattct ttggcagttt
     3841 gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca
     3901 tgtcgcgaag ttcgatctct gtttcttcga tatccaagac acgcatgtcc agacctagtg
     3961 attgcaattc tttcaccaat acgcggaatg attccggtac acctggtttt ggaattggtt
     4021 cacccttgac gattgcttcg tatgttttca cacgtccgac tacgtcatct gatttgtatg
     4081 tcaagatttc ttgtaaagta taagccgcac cgtaagcttc cagtgcccat acttccattt
     4141 caccaaaacg ttgtccacca aattgagctt tacctcccaa tggttgttgt gtaaccaatg
     4201 agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac catgtgagca agtttgatca
     4261 tgtacatcac accgacagac acacgtccat caaatggttc acctgtacgt ccgtcgtaca
     4321 gaacagtttt cgcatcgctg gccataccgg cttcacgaac tgtttcccat acgtcttcat
     4381 cacttgcacc atcaaatact ggcgttgcta cgtggatacc taactgacgt gcagccatac
     4441 ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga aggcacccct aaaggattca
     4501 gcatgatatc gattggtgtt ccatcaggta ggaatggcat atcttcttcc ggcataatac
     4561 gggatacaac ccctttattt ccgtgacgac cggccatttt atccccttca tggattttac
     4621 gtttttgaac gatataaaca cgaactaaca tgtttacgcc tggtgacaat tcatctccag
     4681 cttcacgagt aaagattttc acatcgtgaa cgataccgcc gccgccatgt ggtacacgta
     4741 atgatgtatc gcggacttca cgagcttttt cgccaaagat cgcatgcaat agacgttctt
     4801 ctgcagataa ttctgttacc ccttttggcg tgactttccc tacaagcaaa tcgccatctt
     4861 ggacttctgc accaatacgg atgataccca tttcgtctaa atcttttaat gcgtcttccc
     4921 cgacattagg gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg cgggcttctg
     4981 attcatattc ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa cgacggctca
     5041 tgatgatcgc atcttcatag ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt
     5101 ggcctagagc catttcgcct tgttccatag atggtccgtc tgccagagta tccccttttt
     5161 cgactttctc acccaaatga acgattggac gttggttgta gcttgttccg gagtttgaac
     5221 gacggaattt tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt acgcggattt
     5281 cagaagcatc aacgtattca acgacaccgt catgtttaca taacaaagca gctcctgagt
     5341 catgcgctga tttgtattcc ataccagtac caacccatgg tgcttgagga ttgatcaacg
     5401 gcactgcttg acgctgcatg ttggcaccca tcaaggcacg gttggagtca tcgttttcca
     5461 agaaaggaat acatgctgtc gcaacggcaa ctacttgttt aggtgatacg tccatgtagt
     5521 ctactttatc aatagatact tctaagtttt cactttgagc acgagccata acaacgtctt
     5581 gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg ggctacgatg taatggtctt
     5641 cgatatcagc tgtcaagtag tcgatttggt cagtaacacg acctgtttca cggtctacac
     5701 gacgataagg tgtttcgata aatccgaatt tatttacttt cgcataactg gacaaactat
     5761 tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg acacatacgg ccatagtggg
     5821 agtagtgaac gtcacgaact tcataaccgg cgcggtcacg agtcaatccg ccaggtccta
     5881 aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg gtttgtttga tccatgaact
     5941 gtgacaactg agaagaacca aagaattctt tgatacttgc cacaactgga cggatattga
     6001 tcaattgttg aggtgtcaat gtttcagtat cttggatcga cattctttca cgaaccacac
     6061 gttccatacg tgctagcccg atacggaatt ggttttgtaa aagttcaccg actgaacgga
     6121 tacgacgatt tcctaagtgg tcgatatcat cgacattgcc gataccttcc atcaagttca
     6181 agaaatagct cattgaagca atgatgtcgg ctggacgaac cgtttttaca gctgcttctg
     6241 gatagccgtt tccgatcacg tttacttcac gttcagggtc tcttggtgaa aatactttga
     6301 tgacttggac agtcattgga tctgtgacca caccgtcttc tgatggatag taagtcactg
     6361 aatttaggcc attatcaatg aaaggagcta gtgtttccat gacttggtga gtcagaactg
     6421 ttcctttttc aacgatgatc tcgcctgttt ctggatcaac taatgtttca gctaatgtca
     6481 gattcaatag acgtgttttc aaatctaatt ttttgtttac tttgtaacga ccaacgtttg
     6541 ctaagtcgta acgttttgga tcgaagaaac gagcattcag caagttacgt gagctgtctg
     6601 ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc
     6661 ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt
     6721 cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga actaatacgg
     6781 ttaaaggaat cttacgtgta cggtcgatac gtacataaga gatgtctttt gcatcagttt
     6841 ccatttctag ccaagctcca cggttaggga taacagtaga accgaatcct tcttttccgt
     6901 ttttgtcgac ttttccatgg aaatagacac ctggtgaacg gactaactga gaaacgatta
     6961 ctcgctctgc cccgttgatg atgaatgttc cttgttctgt cattaatggg aaatcaccga
     7021 agaatacttc ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc aatgttacat
     7081 gtaatggtgc agagtagttt gcatcatgcg cacgagcttc tgctactgta tactttggtt
     7141 ctttcaattc ataatctaca aattccaaag atagattgcc attgaagtca tcgattggca
     7201 aaatgtcttc aaacatttct ctaagtccct catctaagaa ccattggtaa gagtctgttt
     7261 gaatttcgat caaatttggt aattccaaca cttcactgat tcttgcgaaa cttctacgtt
     7321 cgcgatgttt tccgtatttt actacgtgtc cagccaagct cttcacccct ctaaaatgtc
     7381 attcaaaaag actacccgat caattgttac aataatattt caaatattat ttttaggtaa
     7441 caacagacat tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac attctcttga
     7501 aaccttactt cgtgagcttc tacttttgtt tccttatatt aaagccgaaa cggacaatat
     7561 ttcgtttctg ccttttcttc atcagatagt ttttgcaact tcatatacta cacttatttt
     7621 ctataaaagt caacacttcc tggcgtttct tctgcttttt cgcaacattt tcttaatgtt
     7681 ttcaatttta tgttttcaat tatctgacaa ttcttaattg acagaccttt ttatctcggt
     7741 tatactttag aaaattcatt ttgtggagga acttctatgc gaaccaacat caaccaaatc
     7801 gcaccaacac aactgaataa cttttacttt agctacttta gataggtttg tattcatcat
     7861 gatggataca aacgttaaac gacagtttgt atctatgatg gctaaagaac tttgtattgc
     7921 agcaaaaagc cacgtagaca aaatgctaag tggctgagca gtgaaaatac aaagtaaaag
     7981 tatcaagcat tctacttttt caatttattt tccgactttt caatccattt ggacattttg
     8041 tctgaatgga ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa
     8101 tcatcggatt aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa aaagacaatt
     8161 ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt gcaacttgta caacataact
     8221 cgttagatgc tgcctataaa ggattcaaag aaggattagc agaagaaggg tataaagaag
     8281 gcgataatct aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta
     8341 tgagtgagaa actcgtcaaa gaagatcctg atttgctact tggaatcgcc actccagcag
     8401 cacaatcttt attaaatgaa acaaccacta tcccgatgac tgtcacagcg gtcacagact
     8461 tggaacaagc ccatttagtc acatccaatg aaaaacctgg cggcaatgtg actggcacga
     8521 cagatatggt gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca
     8581 aaacaatcgg catcatgtat aacaacggtg aagcaaactc taagatccaa gctgatttgg
     8641 cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt actgacagca aattccacaa
     8701 atgatgtcca acaagtgacg aaaagtctag ctaaagatgt agatggaatc tatattccta
     8761 ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa
     8821 cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg cggtctagca acagtaggaa
     8881 tcgactacga aagcttgggt aaacaaacag ggatcatggc agcaaaaatc ttagacggca
     8941 agaatccaag tgaacttcct gtcgaaacag cgaatgattt ggaactgtat gtcaataaag
     9001 acatggccaa agcattaggc atcaatccag acagcatcaa agaagctgaa taaaacacag
     9061 gaggattctc atgacaaatt tgctctctct tttcttatca tctgcatcgc aaggggtctt
     9121 atgggcccta cttgcgattg gtgtatacgt cactttccgt atcttggaca ttgccgactt
     9181 gactgctgaa ggaagctttc ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg
     9241 ctatcatcca ttgacagctt gtcttctagg ctttttaggg ggtgcggcag cagggcttgt
     9301 ttccggactg cttcacacaa aattgaaaat tcccgccctt cttgcaggga tcatcaccat
     9361 gactggttta tattctgtca catcccgtgt gatgggtgcg ccgaacatct ctttattggg
     9421 tcagccaacg atctttcatt gggtcgagtc attcggcata acaaaagaca acgctgtact
     9481 gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta cttgttttgt tcttacgtac
     9541 agaaacagga cttgctattc gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg
     9601 gatcaacaca gataatatga agatcatcgg ttatatgatt tctaatggat gcattgctct
     9661 ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat ttaaattctg gtattggaac
     9721 gatcgtaatt ggacttgctt ctattatcat cgcagaagtt ttgttacgta accagccact
     9781 atttctccgt ctcttgactg tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt
     9841 agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt
     9901 gatctgtctt tctttcccgc agatccagca aaaattccat ttacctcaaa aaaagaaagg
     9961 cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa
    10021 ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc tttccttaga tcccggcgac
    10081 ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc
    10141 ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa
    10201 tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc aagatcctaa actagggaca
    10261 gccgttcgtt taacggtaga agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc
    10321 ggcttcttcc gtggtgtcaa accacaagac cgaacatttt tcaaagacca gctggttcgg
    10381 ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa
    10441 cgtcaagcta ttacgttatt gatggccaca ttacagcgtc cagaactgat cttattagat
    10501 gaacacactg cagcgcttga ccctgctact tccaaaacgg tgatggaatt gacagaaaag
    10561 ctgatcactg agcagaatct cactgctttt atggtcactc atgacatgga agacgcgatc
    10621 cgctatggga gtcgactgat catgctgcat caaggaagga tcgtcgtaga tatccagcaa
    10681 gaggaaaaac agcagctcac agtgccccaa ttattagcgt tgttcaaaca aaacagcggc
    10741 acggcattaa aagacgatca agtactttta tcttcataat cagaaagagg atgggacaaa
    10801 aaatgaacgc ccaccctctt cttttttctc tttccttctt tcgtttttgc tttatgtatc
    10861 aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct tcctgataga atggatggga
    10921 aaggagcttg atgccatgac aacgaaagac aaggtacttc aattattgaa aaagacacca
    10981 gattttcttt ccggagaaaa gttggcacag caactggaag tttcccggac aagtatttgg
    11041 aaagccatca aagaattgga aaaggcaggc tatcgttttg agcacgggcc tacagggtat
    11101 cgttacctgc cctctgacgt actagatgca aaagaaatcc agcaagagct acggtcgttt
    11161 attcctgatt tgaaagtaca aacgatgttg acttctgaat ctacgatgaa agatgccaaa
    11221 ctggcagcag ctcaaggaga ttctgtttcg aagttatttg ttgctgatac acaagaacaa
    11281 gctcgtggac gttttggacg gccttttttc gctgaacctg gacgaggaat ctacatgagt
    11341 ctcttgctcc atccagacaa acggtttgat gaattgcctc aatatacgat catcgcagcc
    11401 agtgcctgtg tgaaagccat tgaatctgta accggtcgtt ctgcttcgat caaatgggtc
    11461 aatgatatct acatggatgg aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat
    11521 atggaaagcg gcaggatcag tcatatcatc attgggatcg ggcttaattt ttctgttcct
    11581 caaaatcatt ttccagctga tctccaagaa aaagccatct ctgtctttcc cgacggacaa
    11641 gctagtgtca caaggaatca gctaatcagc aaaatctggc aagaattgtt cacttatctt
    11701 tctcacccag aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt gggaaaaacc
    11761 gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg caaaagaaat cggtgatcat
    11821 ggcgaactga tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt
    11881 ctcacatcta tccgtacgca ataattatca gatttcacaa aaggacacct gatctcatac
    11941 agtgtccttt tttctgaatt ctcttcaaaa gactctcctt attctatcag ctgacagttt
    12001 ctctatcctc tagaaaaagg tattctaaaa atatctataa agcatggtac actttcttta
    12061 aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag agatagcagg
    12121 ccgcttcctt tttcaatctt atagatagac catgggtaac gataaaaaca aaaaagaact
    12181 tgcgacttat tttgccgtaa gttctttttt agctagtgcc ttaatctctt aatcccaaca
    12241 aacgtctcgc atagatcacc tgaccagagt gcatagcagc atcatcaata atagaaacga
    12301 gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc aattacttca tctagctcgg
    12361 aaggttccag ccttcttaag taagaaatag caaaatcagc agctcctcgc aagtatccta
    12421 gcagatcttc tatttgatta gcttggatct gctttgcttg ttccagacta tgcttccaat
    12481 cttgttcgtt atctgctatc gtcatcggaa accggctttc ccagtcttga ctatgccaaa
    12541 ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag
    12601 ccagccaagt cattgatttg atctgcgggg ctctatcttc cactggaaaa cgattggcct
    12661 cctcaataga gacgccttca aacaaacgtt caaaccgatc gtatgcccga tccacactct
    12721 cgcatagcat atctaaataa tccatttatt ttcctccttt ttatttatcc tacttacttt
    12781 ttcctaattc taggatacct acttatatgt tactacacac ccttattttt tacactttaa
    12841 cagtcaccat catcaatagt ttggagattg aaagaaaaag atagtgtcga actatcaatt
    12901 gaacaagaag caacaatatg aacgtttcat taatattctt taaataaaca atgatttttc
    12961 aaacgaacag tttaagacta ggagaaaaag agtataatag gaaaatgttt gaaaggaaag
    13021 gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg ttgttcaata
    13081 tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac cctgggaata
    13141 tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa ttccacgtat
    13201 ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca acacatgaac
    13261 ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac caagaaataa
    13321 attatataca aaaaactgat tctttcgcta attcttcagg catacctaca ggtagcacaa
    13381 gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat ggtcatccta
    13441 cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa gatcgtgctg
    13501 ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc
    13561 ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac atgaacaatc
    13621 ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat atcgatgcag
    13681 aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat
    13741 ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa tacaatgatc
    13801 gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg
    13861 agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta
    13921 ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg
    13981 cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga
    14041 cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat tcatca
//
LOCUS       DANKHK010000065        13862 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000065 DANKHK010000000
VERSION     DANKHK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            54..1478
                     /locus_tag="PW109_001821"
     CDS             54..1478
                     /locus_tag="PW109_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2511637.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1777..2484
                     /locus_tag="PW109_001822"
     CDS             1777..2484
                     /locus_tag="PW109_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2511638.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2471..2794
                     /locus_tag="PW109_001823"
     CDS             2471..2794
                     /locus_tag="PW109_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2511639.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2883..3920)
                     /locus_tag="PW109_001824"
     CDS             complement(2883..3920)
                     /locus_tag="PW109_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2511640.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4241..5224)
                     /locus_tag="PW109_001825"
     CDS             complement(4241..5224)
                     /locus_tag="PW109_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2511641.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5639..6409)
                     /locus_tag="PW109_001826"
     CDS             complement(5639..6409)
                     /locus_tag="PW109_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511642.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6402..7337)
                     /locus_tag="PW109_001827"
     CDS             complement(6402..7337)
                     /locus_tag="PW109_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511643.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7653..9302)
                     /locus_tag="PW109_001828"
     CDS             complement(7653..9302)
                     /locus_tag="PW109_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2511644.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9419..12022)
                     /locus_tag="PW109_001829"
     CDS             complement(9419..12022)
                     /locus_tag="PW109_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2511645.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12034..13083)
                     /locus_tag="PW109_001830"
     CDS             complement(12034..13083)
                     /locus_tag="PW109_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2511646.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13109..13423)
                     /locus_tag="PW109_001831"
     CDS             complement(13109..13423)
                     /locus_tag="PW109_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2511647.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13439..>13862)
                     /locus_tag="PW109_001832"
     CDS             complement(13439..>13862)
                     /locus_tag="PW109_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2511648.1"
                     /translation="NNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDREN
                     HMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKG
                     TNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4080 a         2883 c         2376 g         4523 t
ORIGIN      
        1 ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa
       61 ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc
      121 aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag
      181 aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc
      241 aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta
      301 tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt
      361 ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact
      421 tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag
      481 tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt
      541 gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt
      601 ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac
      661 ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg
      721 ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag
      781 cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt
      841 tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga
      901 aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg
      961 agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag
     1021 agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg
     1081 aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca
     1141 tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg
     1201 atacagggca aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact
     1261 tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg
     1321 attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta
     1381 ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa
     1441 atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag
     1501 tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata
     1561 cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag
     1621 gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt
     1681 aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat
     1741 tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa
     1801 actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt
     1861 ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg
     1921 atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg
     1981 ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg
     2041 attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg
     2101 acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac
     2161 tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt
     2221 gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc
     2281 atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct
     2341 aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt
     2401 tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg
     2461 tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc
     2521 aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc
     2581 gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag
     2641 tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc
     2701 agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg
     2761 aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa
     2821 ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt
     2881 gcttatccgt tgaacggacc actaacaacg acttttccta acatatgtcc tgtttctacg
     2941 tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat
     3001 gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc
     3061 gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata
     3121 gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca
     3181 tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat
     3241 acagcgacat actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga
     3301 tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt
     3361 gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg
     3421 cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc
     3481 agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg
     3541 gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg
     3601 tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca
     3661 gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag
     3721 atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca
     3781 gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt
     3841 tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc
     3901 attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata
     3961 cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca tcataattat
     4021 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
     4081 ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa
     4141 aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
     4201 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     4261 ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     4321 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     4381 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     4441 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa
     4501 atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt
     4561 cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt
     4621 gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta
     4681 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     4741 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     4801 cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc
     4861 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     4921 tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt
     4981 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc
     5041 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     5101 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     5161 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     5221 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     5281 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg
     5341 attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg
     5401 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     5461 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     5521 acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact
     5581 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     5641 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     5701 tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc
     5761 cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa
     5821 atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     5881 caaatgtagg cacgatcgat acatcatcat aagactgagc aaaaatcccg ttgagcagtc
     5941 ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     6001 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     6061 tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta
     6121 ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg
     6181 catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg
     6241 aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca
     6301 aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg
     6361 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     6421 gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc
     6481 ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct
     6541 tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct
     6601 tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     6661 ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg
     6721 atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta
     6781 ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact
     6841 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     6901 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     6961 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     7021 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac
     7081 tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa
     7141 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt
     7201 gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa
     7261 agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt
     7321 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat
     7381 ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag
     7441 gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac
     7501 aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta
     7561 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt
     7621 tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt
     7681 tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa
     7741 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
     7801 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
     7861 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
     7921 gcaagccacc gaacgactga agcggaagaa gcaatccgtt caatcgtatt tgtccctttt
     7981 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt
     8041 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
     8101 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
     8161 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
     8221 gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct
     8281 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
     8341 caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc taattctccc
     8401 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
     8461 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct
     8521 tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta
     8581 gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct
     8641 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
     8701 actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact
     8761 tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa
     8821 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
     8881 tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc
     8941 tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa
     9001 cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact
     9061 tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac
     9121 ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa
     9181 ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct
     9241 aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc
     9301 atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa
     9361 agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt
     9421 atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt
     9481 tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt
     9541 cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat
     9601 aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat
     9661 caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt
     9721 tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga
     9781 actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct
     9841 cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc
     9901 gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta
     9961 tgattaattg attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat
    10021 aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct
    10081 ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt
    10141 tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac
    10201 gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca
    10261 gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt
    10321 ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt
    10381 tatatacttt agctgtttct aattttagag atagttctgt atctccttct aatggtgaat
    10441 aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc
    10501 tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac
    10561 ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt
    10621 caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat
    10681 aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt
    10741 ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct
    10801 ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc gttgtgttga
    10861 aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt
    10921 tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa
    10981 cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc
    11041 ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga
    11101 gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg
    11161 ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt
    11221 tatattcagg taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa
    11281 taatattgtg gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa
    11341 tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat
    11401 gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg
    11461 cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt
    11521 tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc
    11581 ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat
    11641 tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg
    11701 tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat
    11761 ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa
    11821 ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta
    11881 aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat
    11941 cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt
    12001 gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac
    12061 ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac
    12121 tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat
    12181 aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac
    12241 tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat
    12301 cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc
    12361 cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat
    12421 agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac
    12481 cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc
    12541 taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag
    12601 tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac
    12661 taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac
    12721 ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc
    12781 aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc
    12841 gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa
    12901 tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc
    12961 tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat
    13021 catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc
    13081 cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa
    13141 tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt
    13201 caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg
    13261 aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa
    13321 tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg
    13381 caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt
    13441 aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa
    13501 actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt
    13561 tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc
    13621 cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc
    13681 ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt
    13741 caaatacgtc tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt
    13801 tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat
    13861 tt
//
LOCUS       DANKHK010000066        13799 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000066 DANKHK010000000
VERSION     DANKHK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PW109_001833"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PW109_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2511649.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PW109_001834"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PW109_001834"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2511650.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="PW109_001835"
     CDS             complement(2169..2906)
                     /locus_tag="PW109_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2511651.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="PW109_001836"
     CDS             complement(2997..3524)
                     /locus_tag="PW109_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511652.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="PW109_001837"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="PW109_001837"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2511653.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="PW109_001838"
     CDS             complement(4401..4886)
                     /locus_tag="PW109_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2511654.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="PW109_001839"
     CDS             complement(5039..5236)
                     /locus_tag="PW109_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511655.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PW109_001840"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PW109_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2511656.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PW109_001841"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PW109_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2511657.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="PW109_001842"
     CDS             9211..9423
                     /locus_tag="PW109_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2511658.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="PW109_001843"
     CDS             9425..11104
                     /locus_tag="PW109_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2511659.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="PW109_001844"
     CDS             complement(11358..11558)
                     /locus_tag="PW109_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2511660.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11854)
                     /locus_tag="PW109_001845"
     CDS             complement(11708..11854)
                     /locus_tag="PW109_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDL2511661.1"
                     /translation="MIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREEGM
                     MEEK"
     gene            complement(12086..12748)
                     /locus_tag="PW109_001846"
     CDS             complement(12086..12748)
                     /locus_tag="PW109_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2511662.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13139..>13799
                     /gene="erm"
                     /locus_tag="PW109_001847"
     CDS             13139..>13799
                     /gene="erm"
                     /locus_tag="PW109_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HDL2511663.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNINKQDNVIEIGSGKGHF
                     TKELVKMSRWVDSIEIDEDLCQVTQKVVKPFQNIKVIHTDILKFNFPKNKDYKIFGNI
                     PFNISTDIVKKIAFESNSKYSYLIVENGFAKRLQNTKRALGLLLMVELDIRVLKKVPR
                     AYFHPKPNVDSVLIVLERHQPLILKKDYKEYQSFVYKWVNREYRVLFTKNQFRQALKH
                     AN"
BASE COUNT         4112 a         2904 c         2355 g         4428 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa ttatatctta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa
    12961 gaaaacgtcc aactgaagtg tcaagtgcaa aattacatat agagctttat tctaaaaagt
    13021 aaacagaaat gatataatca gttacgtaag gaggtttcaa gtatgtgcac tagtatcgca
    13081 gtagtagata ttactttatc tcattcataa tgaaaaaaat ggcaaggaga aggttataat
    13141 gaaccagaaa aacccaaagg acactcaaaa ttttattact tctaaaaagc atgtaaaaga
    13201 gatattgaat catacgaata taaataaaca agataatgta atagaaattg gatcaggaaa
    13261 aggtcatttt accaaagaac ttgtcaaaat gagtcggtgg gtggattcta tagaaattga
    13321 tgaggacttg tgtcaagtca cccaaaaagt cgtgaagcct tttcagaata taaaagttat
    13381 ccatacggat attctgaaat ttaacttccc caaaaacaaa gactataaaa tatttggtaa
    13441 tatccccttt aacatcagta ctgatattgt caaaaaaatt gcttttgaaa gcaattcgaa
    13501 atatagttac cttattgtgg agaatggttt tgcaaaaaga ttacaaaata cgaaacgagc
    13561 tttaggtttg ctattgatgg tggaattgga cataagagtt ctcaaaaaag tgccacgagc
    13621 atattttcac cctaaaccga atgtagactc tgtattgatt gttcttgaac gacatcaacc
    13681 attgatttta aagaaggact acaaagagta tcaatctttt gtttataagt gggtaaaccg
    13741 tgaatatcgc gttcttttta ctaaaaacca attccgacag gctttgaagc atgcaaatg
//
LOCUS       DANKHK010000067        13726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000067 DANKHK010000000
VERSION     DANKHK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(80..2077)
                     /gene="tkt"
                     /locus_tag="PW109_001848"
     CDS             complement(80..2077)
                     /gene="tkt"
                     /locus_tag="PW109_001848"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2511664.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2179..2430)
                     /locus_tag="PW109_001849"
     CDS             complement(2179..2430)
                     /locus_tag="PW109_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2511665.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2577..3200
                     /gene="lexA"
                     /locus_tag="PW109_001850"
     CDS             2577..3200
                     /gene="lexA"
                     /locus_tag="PW109_001850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2511666.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3380..3598
                     /locus_tag="PW109_001851"
     CDS             3380..3598
                     /locus_tag="PW109_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511667.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3887..4399)
                     /locus_tag="PW109_001852"
     CDS             complement(3887..4399)
                     /locus_tag="PW109_001852"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2511668.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4413..6710)
                     /gene="recJ"
                     /locus_tag="PW109_001853"
     CDS             complement(4413..6710)
                     /gene="recJ"
                     /locus_tag="PW109_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2511669.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPRFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6852..7295)
                     /locus_tag="PW109_001854"
     CDS             complement(6852..7295)
                     /locus_tag="PW109_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2511670.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7296..8084)
                     /locus_tag="PW109_001855"
     CDS             complement(7296..8084)
                     /locus_tag="PW109_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2511671.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8081..9022)
                     /locus_tag="PW109_001856"
     CDS             complement(8081..9022)
                     /locus_tag="PW109_001856"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2511672.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9199..9786)
                     /locus_tag="PW109_001857"
     CDS             complement(9199..9786)
                     /locus_tag="PW109_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2511673.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10007..11314)
                     /gene="obgE"
                     /locus_tag="PW109_001858"
     CDS             complement(10007..11314)
                     /gene="obgE"
                     /locus_tag="PW109_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2511674.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11608..12618
                     /gene="gap"
                     /locus_tag="PW109_001859"
     CDS             11608..12618
                     /gene="gap"
                     /locus_tag="PW109_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2511675.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12703..13638)
                     /locus_tag="PW109_001860"
     CDS             complement(12703..13638)
                     /locus_tag="PW109_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2511676.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3771 a         3086 c         2426 g         4443 t
ORIGIN      
        1 tttattcttc taacacataa agaacagctg tctccaactt gcagacagct gttcttatca
       61 gtcaaacaat tgcttggatc tatgatagtt gattgtacgt attcacgaca ttttctactg
      121 taaatccaaa ttcttctaaa attttagtgc caggggcaga tgcaccgaaa tgatcgattg
      181 taatcgttgc gccctctgtt cctacgtagc gttcccatcc aaatgatgca gcagcttcga
      241 tagccacacg tttcttcact gatttaggaa gtacactttc tttatattca gaagattgtt
      301 tttcaaagag gtcaaagctt ggcatagaaa caacagaaac atctttgcct ttttcagcca
      361 attctttttg agctttcaca gccaaatcaa cttcagaacc tgttgcaatc aaaatacctt
      421 ctggtgtttc tccttgtgat ggagaaagaa catacgcgcc ttttttcacc atttcatctg
      481 ctacttcttt tgtagaaggc aatactggaa ggttttgacg actcaataca aggatagtag
      541 gcgcatctgt tgtctccatc gctactttcc aagcagcacg tgtttcattt ccgtctgccg
      601 gacgaatcac ttgaacacca ggcatgcagc gaacagatgc tagttgttca atcggttcgt
      661 gcgttggtcc gtcttctccg acagcaaccg agtcatgtgt taagacgtaa acaacaggag
      721 tatgttggat tgctgctaaa cgaactgctg gtcttagata atcgacaaag acaaagaatg
      781 ttccaccgta gatacgagtt ccgccatgaa gttggatacc attcatagcc gaagccattg
      841 caaattcgcg gacaccgaac cagatgttgc gtccttcata atgttcagga gtgaaatctt
      901 tatctgcagc caccattgta ttattggaac ctgacaagtc cgctgaacca ccccagaagc
      961 ttggtatagc tttcgataat tcttggatga cttctttact tgaaacacgg cttgcttgac
     1021 tgcttccaac ttcataagtt ggcaattctg catcccagtt ttctggtagt ttgccatcaa
     1081 aagcatcttc aaattgttgt gctaattcag gataagcttt tttataattg gcaaacattt
     1141 cattccatgc atcttcagct ttttgtcctt cttctatcat cgtttgatgg aagcgggcag
     1201 ctacttcttc tggaacagtg aaatctggat actcccaacc ataaacttct ttagccatct
     1261 tgatgccatc ttcaccaaga ggagcaccat gaacagcaga tgttccttct ttaggagcac
     1321 catagccgat gactgttttt acttcgatca atgttggttt gtctgtttca gcttttgctt
     1381 cttcaatcgc tttagagatt gcttctaggt catttccgtc tttcactaag atatgttgcc
     1441 agccatatgc ttcatagcga gcaccaacgt tttctgtgaa agcttttgaa gttggaccgt
     1501 ctagtgaaat atcgtttgaa tcgtataaaa cgatcaattt ccctagtttc atatgacctg
     1561 ccatcgaact tgcttcttga gatacccctt ccatcaagtc tccgtcaccg catattgcat
     1621 acgtatagtg atccatcaca ttaaagtttt ctttgttata cgttgctgca aggtgcgctt
     1681 ctgccattgc cataccgaca gccattgcaa tcccttgtcc taatggccct gtagtggctt
     1741 ctacaccatc tgtatgatgt acttcaggat gtcctggtgt tttagaatcc cattgacgga
     1801 attgtttcaa atcatcgatt gtcacttgat agccagctaa atgaagcaag ctatacaaca
     1861 ttgctgaccc atgtcctgcc gaaagaacga aacggtcgcg gtcagcccag ttttttgatg
     1921 tcgttggatt tactttcagg tgttttgtcc ataacgcata tgccataggt gctgcgccca
     1981 tcggcaatcc aggatgtcct gagtttgctt tttggactgc ttcaatactt aatgtacgaa
     2041 ttgtgttaac gccaagttgg tcaattttgt caaacaaaat aacccctcct attaatttag
     2101 gtttttttta cctttatttc ttacactttc attctataag atgtcacacg aaaaagcaat
     2161 ctttttcagt attttttact aaaagttgtc atttcggttg tgtaagcctt ttttcttttg
     2221 gatttctttt aatttttccg gggtcacgtc attgccctca ggatcgacga ctttcattcc
     2281 ttcgatatga tgacgcatac cggaacgaaa agcttccaaa tactctttgc gcaagttgat
     2341 ttgttcttgt tcttcggctt ttgagagacc attttccttc tttttacgtg caagttcatt
     2401 gatccgctct attttttcag gtgacagcat gctcagccct ccttttactc tccttcatat
     2461 tatataaaaa ctgaaaaaaa aacaactaga taaactaaat aaaatacgaa cattcgtttg
     2521 atttagcgct ttttatatgg tactatttat ttatcaagtg aaagaaggtg taccttgtgg
     2581 cccgacaaac tgaatcaaga caaatcgaag tcttacgata tatccatgaa caagtaaccc
     2641 aaaaaggata cccgccgact gtccgtgaaa tcggggaagc tgttcagctt tcttctactt
     2701 ctactgttca cggtcatctt gcacggcttg aaaaaaaagg gtttatccaa agagatccga
     2761 cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga aaagattggg attcgtccaa
     2821 caactattcc agtacttggc gtcgtaacag ctggagaacc cattttagca gtagaggatg
     2881 cttcagactt ctttccaatc cctcctaatc tttcttccga agaaggcagt ctctttatgc
     2941 tgacaattcg tggagatagt atgatcaatg ctgggatatt ggatggtgac aacgtgatcg
     3001 ttcggaagca agaaactgct caaaatggcg atattgtcat tgcaatgact gccgaggacg
     3061 aagcgacttg caaacgattc tataaagaaa aaaacttttt ccgtctgcag cctgaaaacg
     3121 acttgctcga accaatcatc ttagatcaag tatctatatt aggacgagta gtaggacttt
     3181 atcgtagtca tatctattaa ttgctactaa ttaaaagaaa actttttaaa aagagtttga
     3241 gacgttggtc tcaaactctt ttttcgcccg ggttcttctt ctttcaaatt tttatcactt
     3301 ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt gttcgcataa
     3361 attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat taactgtttt
     3421 gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg tgtttgttcc
     3481 gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat ctgagcggcg
     3541 cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc aatcataata
     3601 aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg gtgtgacaaa
     3661 aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt tcggtattaa
     3721 gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg tttccattta
     3781 taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg gtcgctttaa
     3841 aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa tattccatta
     3901 atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc aattcgatca
     3961 aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc gtcgctttga
     4021 ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct ggtttaatgg
     4081 catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc acttcgatgg
     4141 tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct aactcataag
     4201 caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata tcaattcgtt
     4261 tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca ccatctgcca
     4321 tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga atactggcaa
     4381 tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt ctttatggta
     4441 ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg tcgctggtat
     4501 aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa aacaccgttc
     4561 tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa taatttttca
     4621 gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat tcgttcttga
     4681 gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc attcaaatag
     4741 gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga ccgaaaaact
     4801 tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt tggttttctt
     4861 ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc ttcctggata
     4921 atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct accttccgac
     4981 gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag accacccaca
     5041 gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt gatcgaaagc
     5101 tttcctacca catctacctg attattcagc agttcttctt cttgagtacc aaaaccaaaa
     5161 gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg ctgttggttt
     5221 gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaaccg aggcaaagga
     5281 ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac tgttacttca
     5341 tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc aatttgatga
     5401 tcgactatat attggttcat cttctcctgt agtatagtga cattttcact tggcatcgtt
     5461 aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat ctggtcaagc
     5521 atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga tccatcttct
     5581 tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc agcaacaatg
     5641 cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt ctccttattg
     5701 accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc ttcattgatc
     5761 tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc aagtaacgag
     5821 acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc aatcgaaaaa
     5881 ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa tagcgcctga
     5941 agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag gattcgattc
     6001 tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc caaaaattct
     6061 gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac tcctgccaaa
     6121 tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata agcgtctggt
     6181 aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc ttgctgagcg
     6241 tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa ctggattcct
     6301 tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc aaatcgattt
     6361 ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat taccgttgca
     6421 ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc actgattaca
     6481 gcttcttgga ttcggctgat tgctttatcc atatcgtgca tcagatacgg atcatgtaat
     6541 tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta acctctttgc
     6601 cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg gagaaaggat
     6661 tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac tttctcactc
     6721 ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa agaataaaaa
     6781 cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg accatttatg
     6841 aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt tttttgttct
     6901 tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc ctgataagca
     6961 gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt tgcttcttca
     7021 attttctttt gaagttccgt ttgatattct gacatttcct gagtcagctg tttgatttgc
     7081 tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc accaatcagc
     7141 gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc aaaacttaca
     7201 ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa aacaaatcca
     7261 agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa aattccgctg
     7321 gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac ttctaaaata
     7381 aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat ttttgccaac
     7441 ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct tggatcagcc
     7501 ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc tacgccaagt
     7561 ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt cgtggcagaa
     7621 taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt aatgatatga
     7681 ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat cattcctaag
     7741 atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa gtcttcgaaa
     7801 atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc catcaccttt
     7861 tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc ataagcctct
     7921 ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat tcttcgtgcg
     7981 caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc gcccagaccg
     8041 cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct tcctcctcgc
     8101 gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca aacacttctt
     8161 gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg atatgagtaa
     8221 gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga gtcgtggaat
     8281 ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca tgtaccagaa
     8341 tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg cctaaaatag
     8401 tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc gtccgcccat
     8461 cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca gctggaatac
     8521 ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca accacacgat
     8581 aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat tgctgatcag
     8641 taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt aaacgcgttt
     8701 gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg taaatctcta
     8761 atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt ccaaaaatat
     8821 gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga atagtcgttc
     8881 gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca ttgcgctcgt
     8941 ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca cctgcacctg
     9001 ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc tttaatttta
     9061 gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta aaaataacgt
     9121 tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa cccgtttgag
     9181 atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt tggaagtttg
     9241 cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt cagcttcttt
     9301 cgcatcatgc acatcttttt cgacatcttc ataatccaag cgggtaattt ccccgcctaa
     9361 ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa tcaagatcag
     9421 tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct gggcatcttt
     9481 tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc ctaataaaat
     9541 ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg tagaagattt
     9601 gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc cattaggatc
     9661 atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta actttttgat
     9721 ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga ttactcgttt
     9781 gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt aattgtaaaa
     9841 taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa agtcgtatca
     9901 cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc acaacctccc
     9961 ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat tcgacaaatt
    10021 caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt gcccgaagag
    10081 cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt tcatcatgat
    10141 cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc cacgttgcat
    10201 ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag tgtacagttt
    10261 cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac aaatctgctg
    10321 tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga aatactggga
    10381 tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat ttcactagat
    10441 tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga cgctctagca
    10501 agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca taaggatctc
    10561 gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt tcgatatgac
    10621 gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag cctggcaagt
    10681 ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta ggtacgagcg
    10741 tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat agtaaagtcg
    10801 atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc aattctagtt
    10861 cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca ggattccgag
    10921 gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca actagtgttt
    10981 gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg acagtggttc
    11041 cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct ttactcatcc
    11101 cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc aatgtacgta
    11161 acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg ccagccggtc
    11221 cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg cccttgccag
    11281 ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc tcctattatt
    11341 catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact tattttatcg
    11401 gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct tcttttttta
    11461 tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt gacaaaatat
    11521 tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcg gtttcacagc
    11581 acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa cggatttggg
    11641 cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat tgaagtagta
    11701 gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt tgactctact
    11761 catggtactt atcctggcac agttactgca acagaagact caatcgtagt ggatggcgaa
    11821 gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa agaaaacggt
    11881 gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc aaaagcgcat
    11941 ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat gaaaaccatc
    12001 gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc agcaggttca
    12061 tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt tgggatcgaa
    12121 gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt agatggacct
    12181 gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat cccacattct
    12241 actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa attacaagga
    12301 cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc agtattgaaa
    12361 acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct
    12421 tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac aactcaaggt
    12481 tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca acttgttaaa
    12541 actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg cttactagaa
    12601 aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg actggatcca
    12661 gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa catattggct
    12721 taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg cttccgcatg
    12781 ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat agaccttatc
    12841 taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca ttgggcgctg
    12901 gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag cacggacttt
    12961 tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca tatcgtctag
    13021 ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga ttgctttcac
    13081 attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca tgctgacacc
    13141 gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta tccactgtac
    13201 atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt ttccatgtcc
    13261 tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg cgtaattggt
    13321 catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt cacttttcgt
    13381 ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga tggtccaatc
    13441 gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg atgtctgtgt
    13501 tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag cataattata
    13561 aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc ctaaaaggat
    13621 tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat aaccttgaat
    13681 tagtataatc aaagaatctc tactatgcaa tgttcatttt tataac
//
LOCUS       DANKHK010000068        13181 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000068 DANKHK010000000
VERSION     DANKHK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(334..696)
                     /locus_tag="PW109_001861"
     CDS             complement(334..696)
                     /locus_tag="PW109_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511677.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="PW109_001862"
     CDS             complement(770..1150)
                     /locus_tag="PW109_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2511678.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="PW109_001863"
     CDS             1214..2407
                     /locus_tag="PW109_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2511679.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="PW109_001864"
     CDS             complement(2484..3068)
                     /locus_tag="PW109_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2511680.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="PW109_001865"
     CDS             3269..4267
                     /locus_tag="PW109_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2511681.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="PW109_001866"
     CDS             4379..4888
                     /locus_tag="PW109_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2511682.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="PW109_001867"
     CDS             complement(4918..5577)
                     /locus_tag="PW109_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2511683.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="PW109_001868"
     CDS             complement(5579..6343)
                     /locus_tag="PW109_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2511684.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="PW109_001869"
     CDS             complement(6351..7028)
                     /locus_tag="PW109_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2511685.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="PW109_001870"
     CDS             complement(7065..8450)
                     /locus_tag="PW109_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2511686.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="PW109_001871"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PW109_001871"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="PW109_001872"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="PW109_001872"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2511687.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="PW109_001873"
     CDS             9389..10246
                     /locus_tag="PW109_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511688.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="PW109_001874"
     CDS             complement(10327..10662)
                     /locus_tag="PW109_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511689.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="PW109_001875"
     CDS             complement(10729..12099)
                     /locus_tag="PW109_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2511690.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="PW109_001876"
     CDS             complement(12177..12713)
                     /locus_tag="PW109_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2511691.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12959..>13181
                     /locus_tag="PW109_001877"
     CDS             12959..>13181
                     /locus_tag="PW109_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511692.1"
                     /translation="MTFHSQFLSNYIQKQNKKEVFMLIEARKKELLVLQMVIQKNNCS
                     LQELSNDLHIPKRTIKELIRKINVSLKQFL"
BASE COUNT         3992 a         2651 c         2171 g         4367 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta t
//
LOCUS       DANKHK010000069        13135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000069 DANKHK010000000
VERSION     DANKHK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..791
                     /locus_tag="PW109_001878"
     CDS             <1..791
                     /locus_tag="PW109_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511693.1"
                     /translation="GLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPV
                     KSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIH
                     ANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMS
                     NKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVV
                     ITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(936..1676)
                     /gene="truA"
                     /locus_tag="PW109_001879"
     CDS             complement(936..1676)
                     /gene="truA"
                     /locus_tag="PW109_001879"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2511694.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1867..2502)
                     /locus_tag="PW109_001880"
     CDS             complement(1867..2502)
                     /locus_tag="PW109_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2511695.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2649..3890)
                     /gene="sstT"
                     /locus_tag="PW109_001881"
     CDS             complement(2649..3890)
                     /gene="sstT"
                     /locus_tag="PW109_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2511696.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4088..5086
                     /locus_tag="PW109_001882"
     CDS             4088..5086
                     /locus_tag="PW109_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511697.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYSVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5099..7117
                     /locus_tag="PW109_001883"
     CDS             5099..7117
                     /locus_tag="PW109_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2511698.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7177..7893)
                     /locus_tag="PW109_001884"
     CDS             complement(7177..7893)
                     /locus_tag="PW109_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2511699.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8120..8662)
                     /locus_tag="PW109_001885"
     CDS             complement(8120..8662)
                     /locus_tag="PW109_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2511700.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8667..8894)
                     /locus_tag="PW109_001886"
     CDS             complement(8667..8894)
                     /locus_tag="PW109_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2511701.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8973..10853)
                     /locus_tag="PW109_001887"
     CDS             complement(8973..10853)
                     /locus_tag="PW109_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2511702.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11042..11656)
                     /locus_tag="PW109_001888"
     CDS             complement(11042..11656)
                     /locus_tag="PW109_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2511703.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11798..12358
                     /locus_tag="PW109_001889"
     CDS             11798..12358
                     /locus_tag="PW109_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2511704.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12663..12977)
                     /locus_tag="PW109_001890"
                     /pseudo
     CDS             complement(12663..12977)
                     /locus_tag="PW109_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4111 a         2584 c         2072 g         4368 t
ORIGIN      
        1 tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt
       61 tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta
      121 accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat
      181 ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat
      241 ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt
      301 ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct gttgctatag
      361 ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga
      421 ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga
      481 gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac
      541 ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat
      601 tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta
      661 cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt
      721 tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac
      781 attctcacta gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa
      841 agtctttttt tatatttctt taatttgctt catacattat ttaacatcga tatttatact
      901 gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa
      961 aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt
     1021 tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata
     1081 atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc
     1141 tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat
     1201 gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg
     1261 tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc
     1321 acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag
     1381 cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata
     1441 aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct
     1501 cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc
     1561 atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt
     1621 tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt
     1681 tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa
     1741 atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt
     1801 tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct
     1861 caagttttac tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa
     1921 aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg
     1981 aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta
     2041 taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa
     2101 caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt
     2161 ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc
     2221 atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc
     2281 agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag
     2341 aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg
     2401 atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc
     2461 taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca
     2521 attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct
     2581 atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata
     2641 tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca
     2701 cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt
     2761 aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc
     2821 atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa
     2881 ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact
     2941 ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa
     3001 atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa
     3061 gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca
     3121 gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga
     3181 tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac
     3241 agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca
     3301 gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc
     3361 acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct
     3421 gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc
     3481 gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca
     3541 ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta
     3601 aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag
     3661 atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc
     3721 gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc
     3781 aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata
     3841 ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc
     3901 tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc
     3961 taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca
     4021 cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg
     4081 ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa
     4141 ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat
     4201 attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta
     4261 tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta
     4321 ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat
     4381 attcctaata gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa
     4441 tattcgtgat tattggctta tcctaacacc tatcagtcag aattacttta cttattctgt
     4501 ttcttgtact ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc
     4561 gaaaacaaca attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac
     4621 tctatttaat aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta
     4681 tttattttta atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa
     4741 gcttcctcaa gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca
     4801 aatttcactc atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc
     4861 tctcttcgca atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa
     4921 attaggtata aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat
     4981 atgtaatttc cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa
     5041 ctcagggaag gcatggctga taaatatcgg gcagtttttt atttgatatt tattagcagt
     5101 gtccctccta gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat
     5161 cgaatacttc gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca
     5221 ttggcttttt gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt
     5281 cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt
     5341 aaaaatattt actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt
     5401 tttcttctta ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat
     5461 cattaactta ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt
     5521 ctttccttca gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc
     5581 agtcttatta atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt
     5641 acaacgtaga ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag
     5701 tatcgctgta ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc
     5761 ttctttcttg ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc
     5821 cgtaaaagat aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga
     5881 agaacctgct ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa
     5941 ggcagatgaa atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa
     6001 tttaagtgaa agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc
     6061 tattccttac atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg
     6121 ttatggtggc ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa
     6181 cctgtctgct tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc
     6241 aaatattacc gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta
     6301 tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc
     6361 ggataaatta acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc
     6421 atacaaagaa accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt
     6481 atccactatg caaaaccata tgccttatga tgattactat agtgaattga actacactgc
     6541 tgaaggaact gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct
     6601 tcactatacc gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc
     6661 gattaccttt gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa
     6721 aaaatatgga ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg
     6781 tgaacaagca gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc
     6841 tcttgagcat gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa
     6901 tcatctacct gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa
     6961 acaggtcttt gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca
     7021 aaaagaaatt ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta
     7081 cagtgcaaca tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg
     7141 tgataaaaga catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat
     7201 ttgattaaag ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc
     7261 gggattcccc tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa
     7321 tagatagacg tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat
     7381 ttgatagtct gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc
     7441 aatagccatc ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc
     7501 cacactcatt tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt
     7561 cataaatgcc tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc
     7621 gctgctttct gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt
     7681 tgtttcagtc gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc
     7741 tgcagttgac ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt
     7801 ttcttttgtt gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc
     7861 tagaccaatt gttactaaaa tccttttttt caattaaaaa actcctttcg attactccca
     7921 cttctttatt ctatcaaata agcaatgtga ttttattgat ttttcgtatt ttctcatcaa
     7981 tctttcaaat cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa tacatcagcg
     8041 ggtgcttctt ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta
     8101 tttgaagaaa catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag
     8161 tttttccttg gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa
     8221 caagcaattg ttgcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga
     8281 taaagacatt ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta
     8341 aatatttgtt ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag
     8401 acggaacgat ttcattttca atcactaact catgagagat tttattgaac catctcgctt
     8461 cttgatcagc atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat
     8521 acaattgtgc ttggatcaat ttcatttctt ctaatttaaa atttgctaat acatgcccca
     8581 ccatggcgcc agcagttggt ttatgatggt cgatatctgc ttgttttgtt tcttgaacaa
     8641 atttttcttc cactatattc attgatttac gcctctttta cttttgctga ttggacttca
     8701 taacctaatt tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag
     8761 tttgctttga ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga
     8821 gaaaccgcat gttcgatttt ttgcatacag cttggacaag ctagtgtatc taattgcata
     8881 ataatttttt tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca
     8941 gcttccataa aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt
     9001 ccaaaataca ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg
     9061 aacatcccac tagccatgta aataaacccg gcaaataagc cgttgagtaa aaataagacg
     9121 acaccaatgg caatcactac attttcacgg gtatttaaaa cagttttctt agccaagcgg
     9181 taggcatgaa ccaacgattc aaaattagat tgcatcaaga caacgtcact cgtttcgata
     9241 gcaacatccg ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa
     9301 tcattgattc catctccaat aaaagcaacg cgcaatcctt gttcttggta ttttttcaca
     9361 aattgaacct tttcgtctgg taataattca gcatggactt catcaatgcc taacatatca
     9421 gctacataat cagcagtcat ctgattatca cctgtcagca tcactaactg ttttgctcct
     9481 gcttgtttca acttagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacct
     9541 atgatttgag tgattttttt gtcgattgct acaataatga ccgagctgcc tgtttgttgt
     9601 aattgatgta aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat
     9661 ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg
     9721 attgtctgat tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa
     9781 tggatcacgg cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct
     9841 aagctgtccc cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg
     9901 gtcaatgtac cagttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct
     9961 tcaccgcctt tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa
    10021 acaggcgcac caatgactaa agcgccagga caaccaagaa ccaaaacagt gattgctaaa
    10081 cgaaaatcac gactaatcag accaacaacc aacgcaataa gtaaaacggt tggagtataa
    10141 tactgagaaa atcgatcaat gaatttttcc gcatgggatt tggtatcttg tgcttcttct
    10201 actaattcga taattttggc aaaggtcgta tcatctccga ccttagttgc ttcaatcgtc
    10261 acgtagccat ctgctaaaat cgttccagag aagacttcat ctccactgat tttgacgact
    10321 tgtcgtgatt caccagtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt
    10381 ccgtccacag gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca
    10441 tcgatttcca cttcttcagt cgttccatct tcttggacaa cactagcagt agatggggcc
    10501 atctcggtca aagccttgat tgactcccgc gttttggtca atgttttttg ttccagatag
    10561 ctaccaaaca aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag
    10621 gcaccaagca cagcaatagt aacgagtaaa tcaattgaga taactttgac tttcactgcc
    10681 tgatacgcat ggatcataat aggtatgacc gcaataatag aagctatggc tagagcaatg
    10741 ttatagaaca agacaaaacc agtaaatttt ccgataaaac caaaaataat caacacagct
    10801 gataaggcgg tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc
    10861 cctcttttcc tttcattctt acgagactaa gtatagatac ttatccatca taaaaaattg
    10921 accactatca agtttagtca aaaaggcggt tttcttttgg tagaatacac tacaaaaaat
    10981 agttagcaag aagactcact aaaagtgatc ttgccaacta ttgtcgtttc tatcgaaatt
    11041 ttcattgaat gattttcctg tacagcatac atcggagatc tatttcttta tctaagattt
    11101 ttatcttgct aattttttcc tttagtcttt caaatccacg tcgttcatag aattgatacg
    11161 tacagccact atctgtaaaa aggaatattt cttttccttt ctctcttttt tcaaattctg
    11221 ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg atttgcagct aaaaaaatga
    11281 tctctccatc tggcgaatgc ttctttaaat atgctgcaag catttgttca tttgctgtct
    11341 cgtaagctcc tttactttct ttagcaaata tattttgtaa aaaatcgaat atcttaacat
    11401 atatttgctg tccaaaagaa cgatgtttct ttttttctcc ttttatctcc gccagcaaaa
    11461 cacctgccaa ctcgtcctct gcatacaaag caagaatttg agttgctcga ttcaactcca
    11521 tatacaaaaa atatcttccg taaagattca gcagaagttc attttccata tatctctcaa
    11581 agttcattcc ttcaacagca aaatagatag cttttttgta atctttcttt tctaagggtt
    11641 taaaagttag tttcatcatt tcgcctcctc aacttcaatg attgtgttat acttacatta
    11701 taatagacac agtgtctact atctataatc aatcatctat aagtttgtcg attaagcgac
    11761 agctttgctt tgatatgtcg gataaggaga taggtaaatg aaaaagacag atagacgcgt
    11821 caaaaaaaca gaaaaagctt tggctgaaac actatccaca ttattagtaa ataaaaagat
    11881 tcaagcaatc actatacgcg aactaacaga aacggcagat gtacatcgtt cgacttttta
    11941 tacacattat aaggatatct atgatctata tgatcaatta gaaagcaact tttttcggga
    12001 tctaaatgaa attctctctt atgatccagc ccatagctat gaggaactat acactcgctt
    12061 gattgactat gtttacgcaa atactttttt atatcgctta tttatcctca accatggtga
    12121 tatttcgttt cagaagaaag ttaaacaagt tattgaagaa aactatctga acatatggct
    12181 atatgaagac aagaaaacga ttatcacaga agaaatgaga ttctttacta cgtaccacgt
    12241 ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc aattacaagt actccaaaaa
    12301 agaattatta gaactcttac agaaactgaa tgatcacata gagaaaatca tgccttgaat
    12361 aaaaacatac taagcttagt taagaagaac cgtaaccttc tcgtataaca actgaatctt
    12421 atttagccaa attgtttact gaagattttt tgagtaaaat ttctataatt cctacttatt
    12481 gaaaagtagt ttgtagtgct gcttctccct ctctaagtga tatatcttgt tgtaccaatc
    12541 aaattcacct catcattaat aaattacttt taggctaatt tattacaact tataggttga
    12601 agctgtaacc aatttatagt catattatat ttttatcatc cagtttattg aattcttctc
    12661 gtttacctca attcattttc aaggggaatc agctcatcat ctaacatttt acgccatgct
    12721 cgcttcattt cttgtgggta tttaaatggt ttcgcttctg gatgtttggc tttatatcct
    12781 tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc acctggcata
    12841 tattgcagat tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca
    12901 tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca
    12961 ttgctatcta gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa
    13021 agatttttga ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt
    13081 ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc caattctcta taaag
//
LOCUS       DANKHK010000070        12897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000070 DANKHK010000000
VERSION     DANKHK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..924)
                     /locus_tag="PW109_001891"
     CDS             complement(<1..924)
                     /locus_tag="PW109_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2511705.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLF"
     gene            1116..1469
                     /locus_tag="PW109_001892"
     CDS             1116..1469
                     /locus_tag="PW109_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2511706.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(1543..2199)
                     /locus_tag="PW109_001893"
     CDS             complement(1543..2199)
                     /locus_tag="PW109_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2511707.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            2425..4359
                     /locus_tag="PW109_001894"
     CDS             2425..4359
                     /locus_tag="PW109_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2511708.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(4445..5668)
                     /locus_tag="PW109_001895"
     CDS             complement(4445..5668)
                     /locus_tag="PW109_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2511709.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(5662..7560)
                     /locus_tag="PW109_001896"
     CDS             complement(5662..7560)
                     /locus_tag="PW109_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2511710.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(7554..8672)
                     /locus_tag="PW109_001897"
     CDS             complement(7554..8672)
                     /locus_tag="PW109_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2511711.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(8755..9588)
                     /locus_tag="PW109_001898"
     CDS             complement(8755..9588)
                     /locus_tag="PW109_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2511712.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(9581..10498)
                     /locus_tag="PW109_001899"
     CDS             complement(9581..10498)
                     /locus_tag="PW109_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2511713.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(10725..11849)
                     /locus_tag="PW109_001900"
     CDS             complement(10725..11849)
                     /locus_tag="PW109_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2511714.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            12032..>12897
                     /locus_tag="PW109_001901"
     CDS             12032..>12897
                     /locus_tag="PW109_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511715.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKK"
BASE COUNT         4089 a         2552 c         2097 g         4159 t
ORIGIN      
        1 aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg
       61 tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac
      121 caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc
      181 atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa
      241 aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc
      301 tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg
      361 tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg
      421 ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt
      481 caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa
      541 gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga
      601 aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac
      661 tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg
      721 aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca
      781 tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa
      841 taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac
      901 cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt
      961 gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt
     1021 attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca
     1081 tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg
     1141 aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt
     1201 gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc
     1261 cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga
     1321 ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca
     1381 tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt
     1441 cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac
     1501 aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc
     1561 ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa
     1621 atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa
     1681 gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg
     1741 tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat
     1801 tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc
     1861 acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa
     1921 gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa
     1981 aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata
     2041 agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga
     2101 agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag
     2161 tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt
     2221 ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt
     2281 gctcagatat tgtgaaattt tattctttat tcatttattt atcatttctg caaatctcgc
     2341 agtataatgg aatttatggt agactactaa ttgaaaaata gttttttatt tgaggagaat
     2401 tcacaaagta gaaaagagga ttccatgata ttattacaag ccaatcagat tgctcgccat
     2461 tttggtgcag aagtcttatt tgaaaacata catttagaaa tcagtacagg cgctcgtgtc
     2521 gctttagttg gcagaaatgg cgctggaaaa tcgactttat taaagatcat tgctggaatt
     2581 gaagcacctg atgaaggaaa gatcgctaaa aataaaacag cgactctcgg atacctcgct
     2641 caggacacag gacttgattc caatgaaaca gtttggaacg agatgctgaa agcttttgaa
     2701 caagttagac aaatggaaga gcggatgcgc gagctagaga ttttcattag cgaaggtacg
     2761 ccagacacac ctgcttatag ttcttattta aaagaatacg accggctgca acacgatttt
     2821 tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa
     2881 ttcgattctt cattttatga tcagaaaatc gatactttat caggcggaca gcgcacacgt
     2941 ttagctttag ctcgcatgct tttgcaacac cctgacattt tgattctaga tgaaccaacg
     3001 aaccatttag atatcgagac actttcctgg cttgagaatt atctgcaaag ctatactggt
     3061 gcattattga ttgtttccca tgaccgttat tttttagata aagtagtcaa tgaagtctat
     3121 gaattaagca gaagaaaaat gcgccattac aaaggtaact attcgaaata tctagatttg
     3181 aaagcagaac agttagcaag tgaatggaag gcttatgaaa agcagcaaac agaaatccat
     3241 aaattagaag acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa
     3301 agccgcagaa agacattaga aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa
     3361 caagcaaact ttctattcag tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa
     3421 gatgccgcta tcggatatga taatgtcacc ttatctgaac caatcaatct agatatccgt
     3481 cgtcaagaag cgattgctct agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa
     3541 tcaatcattc accagatccc ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc
     3601 atcggctatt acgatcaagg tcaagctgat ctgcatacaa acaagacgat cctagcagaa
     3661 ttgtgggatg agcatcctac cactccagaa aaagagatcc gaagtgcttt aggtgctttc
     3721 ttattttctg gcgaagatgt agaaaaacca atccctctgc taagtggcgg tgaaaaagca
     3781 cgggttgctt tagccaagtt agcgatgaac aaagaaaatt ttttgatttt ggatgaacca
     3841 acaaaccatt tagatattga caacaaagaa gtattagaaa atgcattgat cgattatgac
     3901 ggcacactgc tttttgtttc ccatgaccga tatttcatca atcggatcgc gacaaaagtc
     3961 gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag actatgatta ttatttagag
     4021 aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac aagatgagcc agaatctgct
     4081 cctgctccca cgaacaaatc tgattattac caaagcaaag aacagcaaaa gcttatccgt
     4141 agccttcaac gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc
     4201 gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat
     4261 gagctgaacc aagatttaga agctgctaga caagatcaag aaacaaaatt gacggaatgg
     4321 gaagaactaa gcttggaatt agaagaatat gaaaattaac gaaaaaacca aaacagaaac
     4381 cattcatgct gttttggttt ttcttttttt attttatcaa acaaaaattc catatttacc
     4441 tttttcacca ataagaagac caattactag tcattctcac taaagcttct aaataatagt
     4501 aatcacccca aatacaaggt gtatttaccc cattattttt gggcttatta taaacagact
     4561 gtgttaaaaa gccatcttct tcttccgttg tagcatatct ttctttcaat tgtcgcataa
     4621 ttttccaaac agcagactgg tataagttct tttctgtttt gtctaataga cctgattcta
     4681 ttaattctaa taatccacaa accatgatca ccatactaga tgtatctttt tcttctctgc
     4741 tagaatcatc aaagatcaaa tcccaataac aaatggtatc ttctggcaac cgattcataa
     4801 aataattggc taatattttg cttaaatcaa aatatttttg ttctttcaaa taacgataac
     4861 ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc ccaacaggaa ttatcactat
     4921 aaccttgatg cgtacttcca tatcttggtt ttccatcttt gacatccata taaaatgtat
     4981 gataagttga accatcttct cttactaaat aatctgctgc attttgaata tgagcctgag
     5041 cagctttttt atatttttga tttccagttt cttcactcgc ccaatacaat aatggaagat
     5101 tcatgcaaca atccatgatc attctacctt gttgtgaagg atcactagga tctccccaag
     5161 cttgtataac tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg
     5221 aagcttcctt catttttcga tttcccgtta ttttataatc tgctacggtt gataaagaat
     5281 acaaaaaacc aagatcgtga tgattagtga taatttgaca gtcaatcctg tggataaaat
     5341 ctatatctgc tttttcagct gtttgttgaa ataaaggatc acccgtcttc tcaaataaca
     5401 accaattaat tcctgtccaa aatccttctg tccaaccaat attgtctctt aatggataac
     5461 gtccttttat aggacactcg cttgggaatt gatactgact gttactgata cgtttgatta
     5521 acctctccgt gtgtctgcga caacgctcga tctcattatt ccattctttt tccattaatt
     5581 ctttataaca ttcatttatc catttttctg gatgtattat ctcttcaaac atcactttat
     5641 caagcatttc cccaccagcc tttagcataa ttttagtctt tttttattgt ttttattttg
     5701 gatagttagc ccggttgtcc catcaaaatc aattttggga cgattgttcc atacttctaa
     5761 actataatcc cgatcttgat gagcaaccac tcctgttccg agccaatggg ttccttttcc
     5821 aaatgtaccc tctaatccag gaataaaagt gttttcccat gtcatcaagt ttgtattggg
     5881 caaacccata atagctgtgg attttcgttt aatagtatcg tcacttaaac agataatacc
     5941 tgaaaagcca gcttcactca ccctatagtt ttccttgtct tcttctatga ttattccttc
     6001 tttctcagga gaacattcaa tagcaaaccc tgtctcatat agaacatatt ctgtatctaa
     6061 ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag ttctgatttt
     6121 tgcctgtaaa gtaggttccc atgtagaggc aagtacctct ttcttacata caatgtctcg
     6181 tacattattt cgaacgatga actgtccata ggttttgatg ctctcattct taaaactggt
     6241 cgtgatatat gtgtccgctc gctgaatccc tagcattgaa tccattgcca tttctgcttt
     6301 caattgattc gctcgcggta tcgaaaaacc aaattgacta gaataagcaa attttaaata
     6361 tttttcattg gtcaatgtat ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt
     6421 atgtccatta tcatgttgta tgagcaaatt ggcttctgaa agaagtttag ttccttcacg
     6481 aataggcata ggcatttctt tagccgtcca atactcatgg tcttcaggta gcgccaacaa
     6541 taaaaaaatc ttattcagcc ataatggaga taacgtacta ttatagggtt cagtcaatgt
     6601 caattgaggg taggtataac caatattcag gatacctttt tcatcaaata tttctttttg
     6661 catccaccat cttatattcc gattgactaa ccccttgatt tcacctaatt taaaaggcaa
     6721 aagatcggcc cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct
     6781 tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa
     6841 atcaatagct cggttcttat atatctggct aatgggcttt tctggttcta gtacgctata
     6901 aatcaaacca taataatgaa atgcaaaagg attataataa tcttggcgac ctcttgaact
     6961 gtctcgatac cagccatttt gaagataaca attttctata ttatcaaatg cttctttcag
     7021 tgctgattct ttaactggat aaccaaattt ttggataatt gttccgacaa ttatcttaaa
     7081 aaattgccaa ttaccatctg cacattcata ttcaaaaatt tgcgagaacc aatgaataat
     7141 ctggcttttt tctcctgtat ctaatttttt ccataataat tgttcatgat gatataacgc
     7201 taataacatt gcgggcattt ctaccatacg ctggtctctt tcatgtattt ctccccaata
     7261 atcgggatgg gaaggatctg ttccagcttt tattcctgat agaattaatt tcaaccatcg
     7321 ttcatctaat tggtcaattg cgggccccgc tccccataaa acccgagaga atccttccat
     7381 tcctgcaatt ttatttccat atcctacccc ttgatgtata taatcaattt gtgcgccact
     7441 tgctcgataa aacggctgga gtggttgatt tatcttttta aataagtcaa agtaatcctg
     7501 tctggtcctt aatagtggta ctggatgttt cattgtattt tctcctcttt ttactaccaa
     7561 atagctaatt ccgactgttc taatcgaagt aagccttcaa taaaaaaata atcagaatag
     7621 atcagtggca cattataagt cagttctcta tggtatgctt ctgctccgcc tccaatgatc
     7681 ccatcttcat ccatattcca atttgcatag tgcttttctg tcttttggag tatattgata
     7741 gcagcttcat gatacatttt tgatcgtaca gaggaaacat atcctgctaa ttccagaaga
     7801 ccgcatgcag cgcatactcc agcactggta tcaatattcg aatcttttac agcaaaatca
     7861 atgagaggaa tataaccagt tttttcgatt ttctgaataa aatttactgc tatcttttct
     7921 gctatagcta gatagtacgg attttttgtg taacgataac ttaacgcgaa tccatataaa
     7981 gcccatgcat ttcctctgct ccaacaagaa ttctcattgt atccttgccc gccaagtaac
     8041 cgaatatatt tccctgattc tgcatcaaat tctgcgatat gcccggttga tccatctttc
     8101 ctcactagat acttagctgt tgtatctgca tgctgccgag caattttaga gaacctcgga
     8161 tcaccagttt ccctcgaagc ccaatacagt aatggtaaat tcatcatact atcaataatc
     8221 atccaaccag gtttatggtc attccatgct actaaatagt tccctgaaat attatagcgt
     8281 ccagcaagta ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt ttgagactgt
     8341 tgataatgtg caatagccga tggtaagaat aaaaaaccta catcatgatg aacgtctgaa
     8401 aaatggtaca gggcagattc taataattgt tcttgttgta ttgctgcctc tttgaacttt
     8461 tctcgttttg tcttgtgaaa gagctgccat aaaataccac tccaaaaacc attggtccac
     8521 cagtcgattc ctctgtcctt tattacgtct gtatattttc cgttgtaaga aatatatgga
     8581 attttattcc ccattctgtc taattccgca tctatctttt cagaagctac attcaaaatt
     8641 tttcttgacc attccttcgt ctgttcattc atggatatcc cgccattctt ttaattttta
     8701 tgaatgtgga gttgctccta gaacgaacta gatggttaac attcgttatt ttttttactt
     8761 atttgcagct ccaatgtcat tctccttaaa tttagactgc gcccacacat aaatcaagac
     8821 cattgggatc actgaagttg ctaaaattag cataattgcg ccataattaa ttggctcacc
     8881 tgtactagaa accgtcatat ttttgataat taagggaacc gtatatttat ctttatctga
     8941 aagcatgact aatggaaata gatattgatt ccaacttccc ataaagataa gtaaaccact
     9001 agtaataatc gtaggtgata aaatcggtac agcgattttc gtaaaaatca cccactcatt
     9061 ggcaccatcg attctggcag cctccatcag tgctgtagga aattgactgc tatattgtcg
     9121 catcataaac actgaagttg tactggcaat cgaagggatg atcacacctg cataagtatt
     9181 cgataatcct aaacgactca taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac
     9241 catcctagat agcataactg tattgaacaa cagtttattc cccataaaac gatactttgc
     9301 taatgcataa cccgccattg tagaactggc tgtagctaaa actgtactga ttacagcaat
     9361 aacaatcgaa ttccaaataa tcaagggata attatatctc gccatgatgg cttgccagtt
     9421 ttttaatagc atatccccaa acttgaaatt gattccgcca cttaaaattt cttgattact
     9481 ttgcgttgaa gctacaatca tatataaata tggcatcaaa aatattaagg caccaaacac
     9541 aataatgacg tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt ttatcacctc
     9601 cgaatttaaa ttgaatagcc gataatataa cagatacaaa caccatcgtc caggcaatag
     9661 cagaggcttt tccaaaatca accatttcaa aacttgtttt atataaattg actcctacga
     9721 ccaaagctga attttctggt ccattggaag tcccaaagaa tatatttggg atattgaaat
     9781 tacccaagcc attgatagtg gctaaaactg tggccattaa aatgattggc tttagcatgg
     9841 ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc atctatttca gcactttcgt
     9901 atatctcttt agaaatccct tgaagaccag ctaaaaataa aatagtgtta tatcctacac
     9961 taagccaaat catcgtgatg atcattgata ttcgtatcca aaaaggattt gttaaccact
    10021 gtacaccgtt aattcctagc gcattcaaaa atccattgaa taacccagtc ggattgaaaa
    10081 aagtcatgaa tacggaagct acagccaccg ttgatgtaat ggttggaata taatagacag
    10141 ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa taaaacagca aagtaaatag
    10201 ctaatagcat cgttattgga attgatcctg ccatgataat caaaacattc gtgtaagatt
    10261 taataaacaa tggatctcgg aagatagcta gataattgtc aaatccaaca aattcaaatg
    10321 agtagccaat tttcataaaa ctaaaagcaa acgagataat gatgggaatc aaaatcgtca
    10381 caataaagaa aataacaaaa ggtgtgacca atccataagc acttttttta tgagacgtat
    10441 ctgttgcttt tttcttgctg ttctttattg tcttgcttgg taacgaagat tgcatcatcg
    10501 ttaagtcctc ctaatatttt ttaatcacca gttaatttta cgccgtactt atgagcaaaa
    10561 ttttctgctt cttttagata tgaattttca aaatcacctg tttgccaata gttatatgca
    10621 gcggtagtca aataagacgt agcatctgaa taagtggctg gtaaaacagt atttgatgta
    10681 ttttcattga ttttattcaa tagttcatag tatttttgtc ctccttagta gtcaaacttt
    10741 ttctcagctg cttctgtttg ataggccaat gagttagatg tagccacgcc cagctccagt
    10801 gcgttggcta agcattcttc atctgtcagc ataaatgtaa gaaattgttg tgccacttgt
    10861 ggatttttgg catctttagg gacataaaaa cttgatcctc ctgatactgc accttttcct
    10921 ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga tgcatcagga
    10981 aaattcaaag acatattcgt agcataccag ccgccagcaa caaacattgc agattcttga
    11041 aatgctttct cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc gatcatttct
    11101 ttgattaact ttgcagcatg tttcgcttct ttagtattca gacggatatt tccttcttca
    11161 tcaagcaaag attgattctg ctgggctagc atgttgataa acaattcttg gtccccagat
    11221 tctggcaagg caattccaaa actaccagtt ttttctttta atgcttttgc cacctggata
    11281 tattcttccc aagaactgat ttcttcaaaa tctacaccta tttcagcaaa tttttttggt
    11341 tgataataac aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt ttcattactc
    11401 gtttgttgac gcaaaatatt caatttttga tcataaaact gatcgccata tttgtcataa
    11461 aatccatatt catcagcact ataaaaagca tcttgaaatt tttccagcac acctgctgct
    11521 gaagtgtctg ccatggagat aatatccggc atatcttcgt gtgttaccaa ttttggggtg
    11581 atcatttgta ctgttgctgt tcccgaagct acattcacaa tttttaagtc aatatttgct
    11641 ccagttttgc ggttatagat ttttgcagca ttttcaacat agtttgtcag tttgccgttt
    11701 gcgacatata cttctaatgt agtggctttt tttatcattg gatctgcgat gtttgctgtt
    11761 acttcttcgt tagtttttcc acatgcactt aagctgaata atagggtaat gtttagcaaa
    11821 gcaatcacca atttgtatct ttttttcatg tctatccctt ctttcttttc aagttcttat
    11881 ataaaacgct ttcacttatg tatattaacg gaattagtaa cacaaatatt tatataaaaa
    11941 aatattttac ttgtcgtttt ggcttttcat ttttgttata atagtcatta attataaata
    12001 gaaagctttt tatataaaaa gagaaaggaa agtggttctt ttgttaacaa acaaccaatc
    12061 ctccaatctt gtcaaaaaaa cactaaccta ttccatagat acccatcttc atttatctgc
    12121 tgctccctat tttcgactac taataaatga aagcgctaat ttatatgctg aatttgcaca
    12181 taaatctata gaactagata atccttccct tttattcatt gataaacagc aagaagtcca
    12241 aatcttttct actcaaaagg aaacacaaac ttttatcatt atccagtttt attttgatga
    12301 gcaattatta gcacatatta cgggacaatc tttttctata gatcaagaaa taacatctat
    12361 cattactttt ctgaaaaatg aacagttcaa aattgattat ctaaaacata gtaagccagc
    12421 tatttcctat gatcaggaaa tcaattcttt tttcatacta tgccaacaga tgagcattca
    12481 tttgctttcc ttattagtta cttattgtct gcaaaaaatt caattgtctt ccttaccaag
    12541 gcattatcaa caggaacttg ctttattgac aataccttca aatgagaaag tcattcctaa
    12601 aaaaaagcat attctttatt cagatgaatt agttgatcca atcatacgat atttagaaga
    12661 acatctttca gaaaattttt tgcttgaaca attagcccaa atttttttta tcagtccagc
    12721 cagcttaaaa aaacatttta gaaaagtgaa tggaaaaagt gttattagct attttaaaga
    12781 actaaaaatg gaaaaagcaa aacgtctcgt gctagaaaat gaattaacct atacagaaat
    12841 tgcagatata ttaggatttc attcgatcca tcatttttct tcagctttta aaaaata
//
LOCUS       DANKHK010000071        12264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000071 DANKHK010000000
VERSION     DANKHK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..275)
                     /locus_tag="PW109_001902"
     CDS             complement(<1..275)
                     /locus_tag="PW109_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511716.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            569..1384
                     /gene="thiD"
                     /locus_tag="PW109_001903"
     CDS             569..1384
                     /gene="thiD"
                     /locus_tag="PW109_001903"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2511717.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1713..3164)
                     /gene="cls"
                     /locus_tag="PW109_001904"
     CDS             complement(1713..3164)
                     /gene="cls"
                     /locus_tag="PW109_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2511718.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3505..6171
                     /locus_tag="PW109_001905"
     CDS             3505..6171
                     /locus_tag="PW109_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2511719.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6535..7287
                     /locus_tag="PW109_001906"
     CDS             6535..7287
                     /locus_tag="PW109_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2511720.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7309..8463)
                     /locus_tag="PW109_001907"
     CDS             complement(7309..8463)
                     /locus_tag="PW109_001907"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2511721.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8648..11089
                     /locus_tag="PW109_001908"
     CDS             8648..11089
                     /locus_tag="PW109_001908"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2511722.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11419..12123)
                     /locus_tag="PW109_001909"
     CDS             complement(11419..12123)
                     /locus_tag="PW109_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511723.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3942 a         2164 c         2564 g         3594 t
ORIGIN      
        1 taaaattgct gattcacata gagtttatga cttcttggaa attctttttc aatcagtttt
       61 ttcatctgtt tatgaaattc ttttttttca gtaaacataa aggtttgggt atcgtaacct
      121 tcttcatgta ggaaatagat tattcctttt tctgtgtttt gcgtataagc ttttatactc
      181 ctgtcttttt tgaaagaaca gatcgtatag ccatctattt ggtctggtag tcgtttgatt
      241 ttcttcaaaa tgactttttt atcctcaagt ttcatttttt tcacctgact ttattttttt
      301 catatggaaa tcatagcgcc tttggttata tgaaaacaag aaggatactg aaatatatat
      361 aaatcaaata gtggttattt attattagaa tagcgaatac aaataaacga ccactatttt
      421 ctgatttatt gcaaatgttt tttcaaataa atttgattac caagaaaagg tagtggtata
      481 tttactgagg gatactttga aaaacatatg atagaaacag taacagtatt atgaaaaatc
      541 tttttataaa aatggaggaa tcgatttgat ggaagatcag tatgtacaag caatgacgat
      601 cgcaggatca gatagcgatg gaagcgcagg gatgcaagca gatatgcata cattctttac
      661 aagaaatgtt tacggtgttt ctgtcatgac cgcatgtgtt gcaggtaatt cttacggaat
      721 cggagcaagt gtgacattgc caacagattt tatcgacaaa gaatttgaac tgattgcgaa
      781 ggactttcaa atacgtgcag ctaaaacagg tatgctagca gattcaaagt tgattgagac
      841 agttgtaaaa aattaccaaa aatatgattt tggaccatta gtcgtggatc ccgtaatcgt
      901 aacgaaacat ggaaatcttt tactagaaga atctgctttg caaagcttga aagaaaagct
      961 tgtccctttg gccgaagtac tgacacctaa tttttatgaa gcagaaaagc tgactggacg
     1021 gacattagca gctgaagaag attttgtgca agcagcaaaa gaactgcaag atatgggtgc
     1081 aaaaaacgtt atgatcaaag gacatcatgc taaaaatgcg cagcaagaaa cagtcaatga
     1141 ctacgtattg ttcgaatcag gaaaatcatt ttggttaaat gcaccatttt atcctaccga
     1201 ccggatcaac ggaactggcg attcgctatc tgcatgtatt gcagctgagt tagcgaaagg
     1261 aaaaacagtt gaacaagcga tccgattggc aaaagactat gtaaaccgtg caattcgtga
     1321 agaaataaat gtagggcaca aatatggccc catcaatcat tgggcagctg gtgaattaga
     1381 gtaaaagaaa gaagttgtga aaaagttgcc ctgcatcaag taataagaac agccaagtaa
     1441 aaacagcttc ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa
     1501 caccgtttaa gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt
     1561 caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga
     1621 gaacaccgtt tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc
     1681 ttagtatatt tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa
     1741 atttgttgct tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat
     1801 gaacaatgtg tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg
     1861 taaatgacag cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa
     1921 cctaccatgc agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca
     1981 tagacaagga tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga
     2041 taaataaaag gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca
     2101 gttgcgattt ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc
     2161 caaacacgtt ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt
     2221 tcgttattcg gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct
     2281 cgatctgctt ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc
     2341 gagacgttcc agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc
     2401 cgtatatgcg tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca
     2461 atattgaaac caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca
     2521 tggtaattca atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt
     2581 ccaccatttt tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca
     2641 tcatataata ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact
     2701 tgctggccga tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct
     2761 ttttttagat cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca
     2821 gaattcattc ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt
     2881 ttgtctttat cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga
     2941 aagttttcta attctttcaa gtcactttgt cggagataga attttttctt atccgttaat
     3001 ccacgtccga aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag
     3061 agccatgccc aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata
     3121 agtgcattta atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt
     3181 acatacaata acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact
     3241 tttcactcct ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt
     3301 aataaggtag gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg
     3361 ttgcgataat atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga
     3421 gacctaagac aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca
     3481 agatcaaaaa atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat
     3541 aaagaaacga tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc
     3601 acgcaagcga aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag
     3661 aagccaactt ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg
     3721 attgtcgcac tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca
     3781 gtattgatgt tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta
     3841 gaagcgttga aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa
     3901 acgattccgg caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat
     3961 gtacctgctg atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg
     4021 acaggagagt cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc
     4081 ggagatcgta caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc
     4141 ttagtgactt caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca
     4201 acaactgagc aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt
     4261 ttggctattt tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt
     4321 ggagaaggta caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc
     4381 gtagccgtag ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg
     4441 ggaacgaata aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca
     4501 cttggttcga caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg
     4561 acagttgttg actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca
     4621 aaatggacag ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg
     4681 atcagtgaag acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt
     4741 gaaaaattaa accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt
     4801 ccttttgact cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac
     4861 ttgtttgtta aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat
     4921 ggtgaagtcg ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct
     4981 ttttcaacac gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat
     5041 ttatcatttg aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct
     5101 ccacgtgctg aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc
     5161 atgattactg gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt
     5221 gaagaaggaa acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta
     5281 aacgaagttt tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt
     5341 atcgttcgtg cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat
     5401 gacgcaccag cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg
     5461 gcaaaagatg cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca
     5521 gtagaagtag gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca
     5581 ggaaatttag gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca
     5641 tttacagcat tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca
     5701 ttaggtacag aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca
     5761 ggaatcttta cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt
     5821 gctgttatct ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg
     5881 tcattttcaa cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca
     5941 caaacagcta tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta
     6001 tgtagcgcgc tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca
     6061 tcagcatttg gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt
     6121 ttgatggaaa tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca
     6181 acgaggttgt gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct
     6241 tctcatgttt gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta
     6301 aggaagaagt tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact
     6361 gtttattcgg aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg
     6421 atagacggaa agccaatcat tttattattc atcaaaagag atagaataga taaaagagga
     6481 gaatgattta ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat
     6541 gaaaaagaat gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta
     6601 actattgctg aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca
     6661 gcagcagacg ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc
     6721 tatgatttga tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa
     6781 aatgagttgc caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat
     6841 gagacatatg aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta
     6901 gaaaaattga tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc
     6961 gatgatgttt tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca
     7021 tcgccagttg taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag
     7081 aaaaaagatt ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac
     7141 tatgacgcgt acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa
     7201 aaacgagaaa tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat
     7261 aaaaaaagaa cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta
     7321 gtaacgaata tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc
     7381 acattctccg tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat
     7441 ccgtgttcga gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt
     7501 ttcttccaga taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc
     7561 gatgcgatct cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta
     7621 taatgaacca gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg
     7681 agccataaga cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt
     7741 ttttcccatc ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg
     7801 acccgacaat tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt
     7861 agataaagga ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat
     7921 atcttctgtc agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat
     7981 catcatcgca acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg
     8041 agcaatatca cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt
     8101 cgccatgtgc aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc
     8161 aaaagattgg attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat
     8221 cccggattct gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat
     8281 tttgctggca gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat
     8341 ttggtcttga ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa
     8401 ctctgtcata tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt
     8461 catttcaaca cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga
     8521 ttaaaagaaa tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa
     8581 ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt
     8641 ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg
     8701 cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt
     8761 agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca
     8821 agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga
     8881 tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc
     8941 acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat
     9001 gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat
     9061 ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac
     9121 cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt
     9181 gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat
     9241 cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag
     9301 tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc
     9361 aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata
     9421 cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat
     9481 tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc
     9541 aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc
     9601 tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc
     9661 aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta
     9721 cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt
     9781 aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa
     9841 aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt
     9901 gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca
     9961 catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca
    10021 aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc
    10081 tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat
    10141 gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa
    10201 tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg
    10261 cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt
    10321 atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat
    10381 tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag
    10441 tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga
    10501 acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg
    10561 ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg
    10621 tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca
    10681 cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga
    10741 taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa
    10801 tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact
    10861 agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt
    10921 tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat
    10981 tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat
    11041 gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat
    11101 ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc
    11161 tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga
    11221 aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca
    11281 taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt
    11341 tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt
    11401 ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa
    11461 aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc
    11521 attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt
    11581 aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt
    11641 tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt
    11701 gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg
    11761 tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga
    11821 cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg
    11881 aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc
    11941 atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac
    12001 ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc
    12061 tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt
    12121 catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa
    12181 aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt
    12241 cccgtttttt gaaagaaaaa taaa
//
LOCUS       DANKHK010000072        11960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000072 DANKHK010000000
VERSION     DANKHK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            30..467
                     /locus_tag="PW109_001910"
     CDS             30..467
                     /locus_tag="PW109_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511724.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFILLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            562..1446
                     /locus_tag="PW109_001911"
     CDS             562..1446
                     /locus_tag="PW109_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2511725.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSMFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKKEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVIIKVLPSHLQVYC"
     gene            1667..4837
                     /locus_tag="PW109_001912"
     CDS             1667..4837
                     /locus_tag="PW109_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511726.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLSLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPCKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRSDGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKIGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGATFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGAGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGKEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     NQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATK
                     IVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYDQRETKSPWILTIKLS
                     QPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQTDYSEYLNNGWAFKL
                     VLTKENQIAGNYSAEMTWTLADAPTE"
     gene            4857..5885
                     /locus_tag="PW109_001913"
     CDS             4857..5885
                     /locus_tag="PW109_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2511727.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVSPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKAYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            5882..6640
                     /locus_tag="PW109_001914"
     CDS             5882..6640
                     /locus_tag="PW109_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2511728.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(6726..7466)
                     /locus_tag="PW109_001915"
     CDS             complement(6726..7466)
                     /locus_tag="PW109_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511729.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7527..8780
                     /locus_tag="PW109_001916"
     CDS             7527..8780
                     /locus_tag="PW109_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2511730.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGVCILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9081..9329
                     /locus_tag="PW109_001917"
                     /pseudo
     CDS             9081..9329
                     /locus_tag="PW109_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            9451..9777
                     /locus_tag="PW109_001918"
     CDS             9451..9777
                     /locus_tag="PW109_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2511731.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9845..10558
                     /locus_tag="PW109_001919"
     CDS             9845..10558
                     /locus_tag="PW109_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2511732.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            10868..11720
                     /locus_tag="PW109_001920"
                     /pseudo
     CDS             10868..11720
                     /locus_tag="PW109_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(11826..>11960)
                     /locus_tag="PW109_001921"
     CDS             complement(11826..>11960)
                     /locus_tag="PW109_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2511733.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT         4066 a         1987 c         2504 g         3403 t
ORIGIN      
        1 tatcaaaaaa tgcacgtaga agtagataaa tggagtggtt ttgcaaattt cttttatttc
       61 tagaagttat tcacgtatac ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa
      121 tggatcattt gtatataaaa tatattcatc aattttccgt tttggtgtta ggcacactaa
      181 ttatagcggg gattgtcgca tttattattc aaggtcgtga catattaaca gtacatcaag
      241 agtttttcag ttatgcattt ggtattcctc tgatcgctat aaggtctgat aacaaaaagt
      301 ggaagaaacg ctattacatt ctcttgctat ttttgtgttt ttatctgcca cctaagattt
      361 ttattttatt aggtatacaa tgcctatcat tttcttttat ttcctatatc attgttttaa
      421 ttttcctcag tttcatagta tgggtgtttt atcttgtcaa aaaataaatg aatgtttcat
      481 gaacagtact tcatcacagc ctaatttttt tctttatgct atcgttatat taaagaaatc
      541 aatcagaagg gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc cagttcaggc
      601 ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa aattggaatc ggtgtttgat
      661 gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg
      721 gctacggaag ggtatcatag tatgtttgtc atgggcggag atggaacagt caacgaagga
      781 atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg gatttttccc attgggtacg
      841 gtgaatgact tggctcgagc attagggata ccgctagaac ctgaggaagc gatcaatcat
      901 ttttcgattg aatcagtcaa gccattggat attggaaaaa taaatgacga ctattttatg
      961 aatgtcgtcg caattggctc tatccctgaa gcaatcaatg atgtagatgc ggagaagaag
     1021 acgaaatttg gaaaactagc ttatttcatg tcaggaatca aacagttagc ttccacacaa
     1081 tcctactcgt ttcacgtaga agttgatgga aagaagaaag aaattgagag cagtaccttg
     1141 ctgataggac taactaactc agtcggcggt tttgaaacat tacttccaaa tgcaaaagtg
     1201 gatgatggta agctccattt ggtttattta aaagattctt ctttgatcga tacgcttaaa
     1261 acattgccag acctattaaa aggagtagat gaatctacga aaaatctagc ctatcttact
     1321 tgtgagcaat tagttgtgtc attagctgat gaggaagaat tgacgacgaa tgttgatggg
     1381 gatgaagggg ataaattacc tgtgataatc aaagtgttac cctcacattt acaggtttat
     1441 tgttaaaatg gaggttgtgg ccgaagagtt gtcaggagaa caccgcttgt tcgggataag
     1501 ggactgtgac aagacttttg tcacagcctt tttttgtaca aaaaagaaat tttcacctaa
     1561 cagaaaaatt ttccataaaa atacatattt aaatatgaaa gtacaattac aaataaatta
     1621 aaacgttaaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatatgt
     1681 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     1741 tgccgatgta gagaaaacaa gcgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     1801 tactttttca gaagaatcaa ttacttctga aactacagaa tcccaaagtg aggcgcctac
     1861 ttctaataca aggcaagaag aaaataagac aagtgagacc tcagaagtag ctgaacaaac
     1921 gtctgatagc agtgaagagg tacaggttag agttgtttca gcggatgggt acagcgaagc
     1981 tgccacaaca gaagaactga cgcagcttct cgcagacgaa tcagtagcta agattcgttt
     2041 aactcagcct ttgactttag acagggaatt ggagatcaag cgagatatcg ttatcgattt
     2101 tggcggactt gctcataatt tcggcacaca tcacatctat atcaatgaga cacctcagtc
     2161 gatcgagttt cagaatttta aaggaacggc agctcatcca ggtgggttga taccaaatat
     2221 cgatggaaat gccattatca ttgcttatat gtccgatttt tggggaaaca gatatcattt
     2281 tacgggagaa ataaaattta ccgggacact tgacttatat gatggttcta aattaagcct
     2341 gatttacgcc cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtat tggatgtcca
     2401 accagtgaaa gaagggatga atgccgctcc gtgtaaagct tatttttgta gaagctatca
     2461 gctaaatgta atcaatggta gtcagcttta tggaccatat ctaggaaaat tctatggttt
     2521 tctagattca ggtgatgaga cgggcagtgg aaaaacagca ccaggactcc atatcttgag
     2581 tggttcaaag gtctctcttg actatgatcg gtcagatggc gtcagcgatg aaggagaggc
     2641 tgtcgatacg ctacccggga atgtcacatt taaagtcagt ggctcaggaa gtgaattttc
     2701 ggtaaataca aagatcaata ttaccaacga caacaatcgc ggaattatcc aaatgagagg
     2761 ctcaggatca agagtcgctg tgtcgaatga tgggaaaatc acaatcaata cagcgactac
     2821 tggcggattt cgcctgcaag gagattctag tcaaatccat gtatcaagtg gtggacaaat
     2881 ctcggtcaaa atggctggag atggcgacca acctggaaac aatgggatgc gttttgtagg
     2941 gaaaggactt tctttgatcg tagaaggagc cggatcagtc atcgatattg ataagcaaag
     3001 tggtcgttct agcgcagttc gttttgagaa cggtacgcaa aaactaacag taactaatgg
     3061 aggctcattg aaggtaagaa atgcagggga tggcaaatct tatgtgaaca gactcaatca
     3121 aggaaaccaa gcgatccaat tttatgacgc ttctggtttt ctagaaagtc cagggtcagc
     3181 tgattttacg gttactggag aaaaatcaaa tatcgatata cgcgcagatt ccggagctac
     3241 cgtagataca actggagata ttgatttgaa ctttatggct ggcgaaaaaa cttatttagt
     3301 catggtcggg aaaattgggg aagataagac tggcatcatc agtggggatg tgttgaatgt
     3361 agacttagaa tctcctacct atttcgattt ccaaaacaaa cggatcggaa gcgagactaa
     3421 tggcataggt ggctgggtat ttcaaggaaa tggcgattct tctttgaaga tcgctcattc
     3481 agaaattgct ttatggcgaa aggcaggagc aacgttcgat aactttgaaa aagatccgga
     3541 ttatttttct gatatgacag acttatcgat caatggaacc gacttaggta attttgtatc
     3601 aagccctagt gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta
     3661 taatcgaatc agtgccaata accagcttcc gatgattgaa tccttaagag taccaacgaa
     3721 tgcagataaa aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag
     3781 agatgcttgg acaggcgaag tagaagtgac tttacgtatt acttatgcag atacgacgaa
     3841 atcacctgta gaaattactg ctctcacaca aggacaaaca gatgaaaatg aaggggcagg
     3901 atataatccg tggggagaag gcgaacaagg cggtttgttt gaggttcaag tacccaacga
     3961 agaaagacta acaacggggg atcaagtgca aatagttgct gctaaaaaag taagaggcgg
     4021 caaagaagtg acagaactgg actctgccaa aacgacagtc gatgtgattc ctcctgagcc
     4081 tgctaaatta agtaccacat tgatcaatgc agcttctcgc tcaatcacag gtacaggcga
     4141 agcaggagct ttggtttcat tgaaaaaaaa tcaagctgtt ttgtccgaaa caactattga
     4201 tgatacaggg aaatttgttc tttctatccc agaaaatcaa ctttttgcag gagatgtatt
     4261 agaaattgta ttaaacgacc aagcaggaga agctgctgct aagggtgtgc agaacaagcc
     4321 aagtaccaac gatgaaattg ggaatcacaa tccaagtgat acgccagttg tttatcatga
     4381 tgccccgcct tttgcacctg ctacgaagat tgtagtagaa ggtggtctct catttcttgc
     4441 cccttccaaa ctcgattgtg gtcagatcaa agcaactggg ttgacgcaag aagcatttgg
     4501 ccaaatggat atacaggaaa aattatggat ttatgatcag cgagaaacaa aaagtccatg
     4561 gatcttaacg ataaaattat ctcagccttt cacaaccgat gcaggatttt ctatgcagga
     4621 tgtcctatat ttcaagaaag ctgaggagta tcaattagtg aatgaagaag aactggaaat
     4681 tgtcagagga caaaattcaa cgaatgtgca aacagattat tcagaatatt taaataatgg
     4741 ttgggcgttt aaacttgtct taaccaaaga aaatcagata gctggtaatt actcagcaga
     4801 gatgacatgg acattagcag atgcgccgac agaataaaga tcaaggagga tgattgatga
     4861 atcgaatatg gaataaaagt catcgaaaca actgctcttt acttctgatc cttctccttg
     4921 gattcatcct ttggcagaca cctgtccatg cctccgaatt caattttgcg gtagattttg
     4981 aattaccggc taatcagatt gataagaaca aaacatatat aaatgtaagg atggagcctg
     5041 ggaaaacaca ggaaatcccc tacacggtct ttaaccgaac agataaatca gtaacagttg
     5101 agacattgat ccactccgct acgacgaatt caaacggtgt gatcgaatat ggagaatcaa
     5161 aaagtaaatt tgatgaaaca ttaagttatg atatgaaaaa cttagcaaaa acagtgggag
     5221 aaataactgt ttctcctcaa agtgcggtca ctgagacact ttcactgaaa gctccagaaa
     5281 aagagctggc tggtgtggtc gcaggaggaa tcacgtttca agaaaaaaaa gagaacgttt
     5341 ctaacaaaag tacagttggt gtcgtcaatc agttttctca agtgaaagca atcttgattc
     5401 gaaatagcga aaaagagtct tccccaaatt tgaaattaaa aaaagcttat gcggatcaaa
     5461 gcgatggaag aaatgtgatt aaggcgaatc ttcaaaatcc aaatgcttct tatatgttcg
     5521 gcttgactac atcggttgaa atctacaaaa aagaaacaaa agagccttat ctttcaataa
     5581 aaaaagaaaa aatagatgta gcacccaata caaattttga tttacttgtc ccattagaag
     5641 gaaaaaaatt agctccggga cattatcaag cggttgtgaa gggtgcttgg aaagaaaaac
     5701 gctggaatct ccataccgat tttgaaataa ctgatgatgc agcggaccaa ttgaatgaaa
     5761 aggatattga tttaaaaaat cagccagaaa catttgactg gcgatggctg atacttgttg
     5821 gagtcattct tattggctta tttgctggat tatttatttt aaaagaaaga aggaaaaaga
     5881 aatgaaaaaa acaattgcat taagtaccgt tctagctgga agtctattat tatgtacaca
     5941 aagtgttttt gctgagaata agagctcagc cactacagaa ggaacgattt ctgtaacaaa
     6001 accagatgcc gcaaatcctg aagacgttgt tccagttcct cctgttgatc caaatgatcc
     6061 cgatacgccg tttacaccta acgaaccaaa tcagggaaca tccggattac tgacgattga
     6121 ccaagctcca gatcttgatt ttggaacaat caagttaggt acagcaaaaa cagtatatgc
     6181 tgcattacaa aacggaacgg acagtacagg gacagcaaca caagtacaga atttcgtaca
     6241 agtaacggat aaaagcggaa tttatgccgg ttggaaacta tcagtcaaac gaacagaatt
     6301 tattggtaca acatctgata cgaataaact aactggttca aaggtgaaat tcaaaaacgc
     6361 cgtgattcga aatagcgcaa ctaatagcag tggaacgcca tctactattt ctggtactgg
     6421 cgctgaagga attgagatcc cagcagatca ggcagtagat ctagtaactg caggagcaaa
     6481 tgaagggatt ggcacatgga tttattcatt aggagagaat gtggaacaag ggagacaatc
     6541 agtggaatta tttgttcctg tatccaatta tgccgtagac aactacgttt caacattcac
     6601 atggtcgtta acagatgctc ctacagaagg gaaagcgtaa aatcaaatca atgacttcag
     6661 ggagtggagc cgattcaagg ccaactccct gaaatctgct atgcttacgt ctcagctctt
     6721 attcatcaac tcgaagcagt tttttggaag tcatggaaac gttcctttgt cttttcaaag
     6781 atctttcgac caacgagtaa ggagtcacca ttgaaaaatt ctaacacata atgagcagaa
     6841 tcaattttct gcaattgcga taagttgata cagtaagatt ttaatagtaa aaagtaagca
     6901 tacttttcca actctgtctt caattgtttt aaagaagtat tcactaattt atcttctgta
     6961 gttgttttga ccataacttg gtttttcaaa cctttgatac tttcaatata taaaatttcc
     7021 cgaggataca ggacgagact tccacctttt tgatcaataa agattttatc gacatttacg
     7081 atttgctgtt ctttccaatt gatctgttct agcacttgac gaatttgttc agataaattc
     7141 ctgtcttttc tgatatatcc aatcggaagg attttccgat tgatgatctc tactgcaaaa
     7201 ttcgtataag cggtgaaaaa aacaatatta ctatgtgcat tttttttacg tatttgttcg
     7261 gcgacatcaa tacctgtgag tgattgcttc aagtcgatat cgataaagaa aacatcgtga
     7321 ttatcaatag tgcccaactg gatcaactga ttgatttgag agatagaagt gagctgcacg
     7381 atattctcga tatgatgatt gaccaattct ttgatgatcc attcgctgtg aaactggtta
     7441 tcttctatga taaaatactt tttcatatgc aggctcctat taaaaaaata tgtgtacatt
     7501 ttgaaacaaa acaatggagg aataagatgg tcgcaacagt gacactttat ttgaccttta
     7561 ctttgatttt agatttaggt atctatttat tcttttcgaa acaactgcta gtaaaaaaca
     7621 gctttggttg tatagctttg atggtggtga cacttttatt tcctatccct aaagtgagct
     7681 ttccttacct agttggctta ttattgatgt ttctttttta ttttactacg aaaaacctgt
     7741 ttatcagctg tttgatcagt aatctttttt ttgctatcac tggactcagc ttttttctgg
     7801 tttttgatct gatggattat ttaggaaaga gacatcttgg aacagcaagt ttttttgcac
     7861 tacttatcgc atttattttc tatctattag taaaaagtat agacaaacag acgagatttc
     7921 tcaacttgct ttttacttat ccaactgaac actattttgg aggtgtttgt attttattaa
     7981 gctggggcgt gatttacttg gtattgggaa gtgttcactt ccgctcatct aattatctgc
     8041 ttctatctct tttgtcgata gtcatcagtt ttttttcttt gtttttttcc attatgttga
     8101 tcagtttcca agtaaaagaa agacaacatc aggaatcgct tcaactgtat caaataaatg
     8161 aagaatatta ccatcatcta gaggaattca aacatgatta taaaagtcta ctgttttcat
     8221 taaaaattac tcttcaggaa aataaaaaag aaacgttgga atttttagaa atcttagaag
     8281 atcattctgc agctattttt gaaaaaccgc aaatacaaca gctaacaaat attgtatccc
     8341 cagctgtcag gggtgtcttt ttaaaatttc tagaaaaagc agaatcggag aaaatcccga
     8401 tccaagtagt tattcctgtc gaagtgaaaa caatcccgat tgatctggtt gtttttattc
     8461 gctgtttatc tatttttatt tctaacgcat tcgatcatcg tgtagccgat cagagtccga
     8521 ttacgatcaa tctgctggaa gaaaaagagg gattacgttt tagtatagcg aacaaggcaa
     8581 atccgacgaa tttgacgcta tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg
     8641 gcgggttagg tctatacagt gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa
     8701 aaatcgaatt ttcaaaacag aatcactatt tttttgtaga aatgtatctg gcatctagaa
     8761 agagcaaatc agcctactga cttactgtca aaggataagc ttgaggaaag tctacacgga
     8821 gatagagaca acaacacagc atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc
     8881 ttctgtttct tttgctatgt agttaaacaa agtagttgac acttccgcta cttagtatta
     8941 gtacgaaagt atacttcatg atactaagta gataagaagt gaaaagcgga acccacgatc
     9001 gtcaaaatgc taactgcttt gcttacacca gataaaggga agagcaatat cgcttatttt
     9061 aggcgtggag aagcaagaaa atccctacga tcacacaaaa aatgctgacc cagcagttaa
     9121 gagagttaga acaagatggc attgtccatc gaaaagtcta taaccaagtc ccgcctaaag
     9181 tagagtactc gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgatgtctg
     9241 tttggggaga agaacgtatc gaaaaacaaa aagcctcagg ggaagaagtc acattactgc
     9301 atacacatgg tggctaccta aattactgat ttctatgaca tttattttaa ataaaagaaa
     9361 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     9421 agtaagaaat gattggaaga aggcaagcaa atgaatagca tggaaaaagt taaccagttt
     9481 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ttctatttct
     9541 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     9601 acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg
     9661 gcagacaacc ttcagctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     9721 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     9781 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     9841 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     9901 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatc ttgtcagaga
     9961 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
    10021 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
    10081 gtcgcagatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
    10141 aatcaattta ccgattttca aaggcttgga taatacgtca ctactttacg gagcaggaac
    10201 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
    10261 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
    10321 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
    10381 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
    10441 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacaccgat
    10501 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
    10561 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
    10621 aaccaatttg ctgaaaaaaa gaggaacgca aagaggtgca tctctgattt ggttgttttc
    10681 tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttac ataaataaaa
    10741 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
    10801 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
    10861 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
    10921 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaag attggggacg
    10981 agatttcgcc agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc
    11041 aattccagca gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag
    11101 tcgaactggc tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga
    11161 ccgatcatct tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat
    11221 tattatttga aacaaaaaaa ttttatccga aagaatatca agcagcgaaa gaggctcttt
    11281 tgttcgtgaa acaaaagacg ggtgtggaac tttctgagga tgaagccggt tttattgcct
    11341 ttcattttgt gaatagccaa caaggaaacg aagacatgca agtgacgatg accgctacca
    11401 caatggtaag agatatactg agtatcatca gtaaattctt cggcatcgtg ttcgatgaag
    11461 aatctttgaa ttatcagcgt atcattactc atctgcagtt ttttgcccag cgttatctca
    11521 aaggggaagg atctgacgag caagatgaat ttctctacgc attagttcaa ggaaagtatc
    11581 caaaagcgtt tcattgtgtc gaacggatca atgagtatct tcgacaaacg caaaaacagg
    11641 aaatggggat cgcagagcag atttatttaa cgattcatat tcaaagagta gtaagtgaga
    11701 aaaaaagcat ccaatcataa ggattgttac tgtatggcag gcaaaacctg gattgaaaaa
    11761 ataggttcac gaatgtttgt gactgttttt ttcaatctgg gtttttattt ttattatttg
    11821 tcaaattaag cgagacaaaa ctttccattt acgtaactca aaaaaacttc taatggtgtt
    11881 tggtaattta gtgattttct agggatatgg tttcttttgg acgcaacgga tgagatgaat
    11941 ccttgattga cttggttgaa
//
LOCUS       DANKHK010000073        11958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000073 DANKHK010000000
VERSION     DANKHK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            23..265
                     /gene="yaaA"
                     /locus_tag="PW109_001922"
     CDS             23..265
                     /gene="yaaA"
                     /locus_tag="PW109_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2511734.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            252..1376
                     /gene="recF"
                     /locus_tag="PW109_001923"
     CDS             252..1376
                     /gene="recF"
                     /locus_tag="PW109_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2511735.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1373..3319
                     /gene="gyrB"
                     /locus_tag="PW109_001924"
     CDS             1373..3319
                     /gene="gyrB"
                     /locus_tag="PW109_001924"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2511736.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3339..5810
                     /gene="gyrA"
                     /locus_tag="PW109_001925"
     CDS             3339..5810
                     /gene="gyrA"
                     /locus_tag="PW109_001925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2511737.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDSAIYGSMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6046..6345
                     /locus_tag="PW109_001926"
     CDS             6046..6345
                     /locus_tag="PW109_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2511738.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6395..6907
                     /gene="ssb"
                     /locus_tag="PW109_001927"
     CDS             6395..6907
                     /gene="ssb"
                     /locus_tag="PW109_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2511739.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6933..7169
                     /locus_tag="PW109_001928"
     CDS             6933..7169
                     /locus_tag="PW109_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2511740.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7312..9285
                     /locus_tag="PW109_001929"
     CDS             7312..9285
                     /locus_tag="PW109_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2511741.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9291..9743
                     /gene="rplI"
                     /locus_tag="PW109_001930"
     CDS             9291..9743
                     /gene="rplI"
                     /locus_tag="PW109_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2511742.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10017..11384
                     /gene="dnaB"
                     /locus_tag="PW109_001931"
     CDS             10017..11384
                     /gene="dnaB"
                     /locus_tag="PW109_001931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2511743.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3994 a         2177 c         2650 g         3137 t
ORIGIN      
        1 taatgaaaat gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac
       61 gttgggccaa atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta
      121 tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact
      181 ttatcccggc atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg
      241 aaacgacgat aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc
      301 tgaacatcga attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa
      361 cgaatctttt ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg
      421 agcaggaatt gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag
      481 gttcatccac tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca
      541 accatatcga acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg
      601 caccagaaga tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata
      661 tggaattagg ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt
      721 tgaaacaaag aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt
      781 atttggatat tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag
      841 cacagttcgt caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc
      901 aaaaagagca gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc
      961 aagagcaaat ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt
     1021 ttcggggaac aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga
     1081 aaaatgtgca gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat
     1141 tagcagaaat cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg
     1201 atgtgatgag cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa
     1261 aagttcaaac gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg
     1321 aacctgagat tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga
     1381 agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga
     1441 aggacttgaa gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg
     1501 tctgcatcac cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt
     1561 tgcaacgaaa atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg
     1621 tcgggggata cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt
     1681 tactgtttta catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact
     1741 tcacggagta ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata
     1801 caaagaagga aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa
     1861 gattatcgaa caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat
     1921 ttttacagag acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc
     1981 tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat
     2041 caatgaatac cattatgacg gcgggatcaa aagttatgtc gaatacttga atgcaaacaa
     2101 aaccgtgatt tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga
     2161 agtctctatg caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat
     2221 ccatacgtat gaaggtggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat
     2281 aaatgattat gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga
     2341 agacgtgcga gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga
     2401 aggacaaaca aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt
     2461 tagcgaacat ttcatgaaat tcttgctgga aaacccaagt gtgggccggc aaattgttga
     2521 aaaaggatta ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag
     2581 acgtaaaggt gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa
     2641 cgatcctgaa aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa
     2701 gcaaggtcgt aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt
     2761 tgaaaaagca agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc
     2821 tatgggtaca gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt
     2881 tatcatgact gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt
     2941 ttatcgttac atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt
     3001 atacggggta aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga
     3061 agagttgaaa caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta
     3121 taaaggactt ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa
     3181 tcggatgatg gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat
     3241 gttgatgggg gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt
     3301 gaagaactta gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa
     3361 acatccatga tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga
     3421 gtgttattgt cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc
     3481 gtattttata cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg
     3541 ccagaatcgt cggagatgtc atgggtaaat accatcctca tggagacagt gcgatctatg
     3601 ggtcaatggt acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg
     3661 gaaactttgg ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga
     3721 tgagcaaaat cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa
     3781 gcaactatga tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt
     3841 tagtgaacgg aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact
     3901 tgtctgaggt agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg
     3961 aattgatgga agtattacct ggaccagatt ttccaacagg tggtttggtt atggggaaat
     4021 caggaatccg aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg
     4081 agattactga gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg
     4141 tcaataaagc aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag
     4201 ggatcacaga tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc
     4261 gtcgtgacgc cagcgcatcc gttatcttga ataacttata taaattgaca tccttgcaaa
     4321 attcatttgg attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga
     4381 aacaaatcct tgaaaactat atcgaacatc aacgcgaagt catcacacga cggacggtct
     4441 ttgaaaaaga aaaagcagaa gcaagagcac acatcttaga aggtttgcgt attgctttgg
     4501 atcatatcga tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaggcaaaag
     4561 caacaatgat cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc
     4621 gtttacgccg attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct
     4681 taaaactgat tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca
     4741 tcaagaatga attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac
     4801 tagtcggtga ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta
     4861 ttacattgac aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac
     4921 gaaggggtgg acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc
     4981 tggtttcttg ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc
     5041 gggcaaaagg atacgaaatt ccagaatacg ggcgtacagc caaaggaatc ccaatcatca
     5101 atttactagg tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag
     5161 cagaagaagg ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg
     5221 tcactgcttt tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg
     5281 atgagctcgt caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata
     5341 acggctactc tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg
     5401 gggtcagagg gatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg
     5461 aaaatcaaga agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg
     5521 aatatccagt gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa
     5581 atggtccact agctggattg accactgttt ctggagatga agacatcatg ctgattacag
     5641 ataaaggtgt cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat
     5701 taggcgtacg tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg
     5761 aaccagaagc tgcagaacaa acagttgaag aacaaggatc agaagaataa ccatcaagac
     5821 taagtgtgta agctagcaag aataaccgat taaaaaatct ggttattcct gctggctttt
     5881 ttgattagtt gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc
     5941 aaaatagagt gtataattag agcttgtgag tagtctaatt aattagataa ctctccttgc
     6001 tcttaggaac ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa
     6061 tatgaaatca tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc
     6121 gaacgtttcg atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg
     6181 gaaaaacgcc gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt
     6241 aaagtatctt ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc
     6301 aacgacgata tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg
     6361 aaacaataga aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg
     6421 acgtctgacg aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt
     6481 tactttggct gtgaatcgta attttacaaa ccaaagtggc aatcgcgagg ctgattttat
     6541 caactgtgtg atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac
     6601 acttttaggt gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg
     6661 tgtatacgtg acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc
     6721 tgaaaatcgc caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg
     6781 cggaaacaat aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg
     6841 tgatacttct gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc
     6901 attctaaacg aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta
     6961 aacgtcgtaa agtagactac attgctgcta accatattga atatatcgat tataaagata
     7021 tcgaattact aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg
     7081 gtacaggcgc taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg
     7141 gcttgctacc tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt
     7201 tgatcagcta ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg
     7261 aatgacgaaa gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat
     7321 aaaaacaatc atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta
     7381 ttggtagtct taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat
     7441 ttatggggtg ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa
     7501 aaaatccagc aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg
     7561 cctgtaggag tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta
     7621 gcattgaaaa tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt
     7681 actgcaaaag aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat
     7741 atggatacag taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa
     7801 ctccatcaga cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat
     7861 gccattgata aattagatga caaagagatt tcttatttga actctttcgt cactacacta
     7921 gtttctgact ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt
     7981 ttcttcgtag cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg
     8041 gatcgctttc gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt
     8101 gcttatggct caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt
     8161 gctttagtcc gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct
     8221 gtttattatg gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg
     8281 atgagtaccg ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac
     8341 aagtttccgg atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc
     8401 aaccagaaaa aagcttacat cgttatcaat gagaacgaga tcattccaga tgtagaacgg
     8461 tgtcttgaag aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt
     8521 gccatagagc tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct
     8581 ctatcgatct ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat
     8641 cgtcgtgggg aagagtttcc aagtaaacct ctgctgactt atattgaatc ttcagcttct
     8701 tcagcttcag agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta
     8761 gatcgttttg aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat
     8821 gtccgtacaa ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac
     8881 gcatccttag tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat
     8941 ttgattgctg gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa
     9001 aaagagtata atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat
     9061 atcaatgcag ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga
     9121 agtaccggca cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc
     9181 acaaacgcag ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt
     9241 gctgcaatca aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa
     9301 tttttttaca agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag
     9361 gttatgcaca aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta
     9421 tcagtcagtt agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag
     9481 aagctaaaaa gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga
     9541 aagcagggga agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt
     9601 taaacaaaca atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc
     9661 gttcactagg ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca
     9721 aagttcatgt cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac
     9781 cagaaggaaa aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa
     9841 aagctgcaaa tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat
     9901 aaagaaaacg agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat
     9961 gacttttctc tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga
    10021 atgaagtgtg gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag
    10081 gctctgtttt tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag
    10141 acttttatcg tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta
    10201 atgaagcgat tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag
    10261 atgccggtgg tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata
    10321 tcgtctacta tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag
    10381 caacaggaat cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg
    10441 atgccgaacg cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta
    10501 ttgctgaagt actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag
    10561 aaatcacagg tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc
    10621 ccgaagaact aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga
    10681 atattgctca aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa
    10741 tgggggcaga atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc
    10801 atttacgtac aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga
    10861 gtctttctcg agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc
    10921 gtgcgaaatg tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt
    10981 atttgcagtt gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct
    11041 ctcgccaact gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt
    11101 tatctcgtgg agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat
    11161 ccggatcaat tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg
    11221 aaagagaagg cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag
    11281 tcatcatcga gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag
    11341 aatacaataa gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc
    11401 cttgagcaat cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag
    11461 agtacttaat ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt
    11521 tttccgaata aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa
    11581 tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg
    11641 acctcttttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat
    11701 caaacatgag aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct
    11761 cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa
    11821 acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct
    11881 ttttcacaac ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa
    11941 tttcacaaaa atttgaga
//
LOCUS       DANKHK010000074        11947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000074 DANKHK010000000
VERSION     DANKHK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            4..2376
                     /locus_tag="PW109_001932"
     CDS             4..2376
                     /locus_tag="PW109_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511744.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDNKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3906
                     /locus_tag="PW109_001933"
     CDS             2437..3906
                     /locus_tag="PW109_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2511745.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPLTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELSR
                     KQTFPQSIEVCVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFRQGRQQERE"
     gene            3917..6121
                     /gene="topB"
                     /locus_tag="PW109_001934"
     CDS             3917..6121
                     /gene="topB"
                     /locus_tag="PW109_001934"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2511746.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEATYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSILQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            6137..6286
                     /locus_tag="PW109_001935"
     CDS             6137..6286
                     /locus_tag="PW109_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511747.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6367..6963
                     /locus_tag="PW109_001936"
     CDS             6367..6963
                     /locus_tag="PW109_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2511748.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6976..7824
                     /locus_tag="PW109_001937"
     CDS             6976..7824
                     /locus_tag="PW109_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2511749.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLQEMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETRETDYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKE"
     gene            7878..8363
                     /gene="ssb"
                     /locus_tag="PW109_001938"
     CDS             7878..8363
                     /gene="ssb"
                     /locus_tag="PW109_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2511750.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASDTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8408..8641
                     /locus_tag="PW109_001939"
     CDS             8408..8641
                     /locus_tag="PW109_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511751.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8800..8922)
                     /locus_tag="PW109_001940"
     CDS             complement(8800..8922)
                     /locus_tag="PW109_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL2511752.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9077..9337
                     /locus_tag="PW109_001941"
     CDS             9077..9337
                     /locus_tag="PW109_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511753.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9783..9989
                     /locus_tag="PW109_001942"
     CDS             9783..9989
                     /locus_tag="PW109_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2511754.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9989..10213
                     /locus_tag="PW109_001943"
     CDS             9989..10213
                     /locus_tag="PW109_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511755.1"
                     /translation="MKTQHKLLTVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10229..10666
                     /locus_tag="PW109_001944"
     CDS             10229..10666
                     /locus_tag="PW109_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511756.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11111..11281
                     /locus_tag="PW109_001945"
     CDS             11111..11281
                     /locus_tag="PW109_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511757.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAKKKQDLRYQH"
     gene            11356..11751
                     /locus_tag="PW109_001946"
     CDS             11356..11751
                     /locus_tag="PW109_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511758.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            11733..>11947
                     /locus_tag="PW109_001947"
     CDS             11733..>11947
                     /locus_tag="PW109_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511759.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATL"
BASE COUNT         4444 a         2007 c         2519 g         2977 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ctttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggataa taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttaatt
     2881 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     2941 catgcggttg cttttttatg gaaagatcca ctgacaggag agacagtagg ggcaagttat
     3001 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     3061 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     3121 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     3181 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     3241 gagtcgattt ctgaattaag tcgaaaacaa acatttcctc aatcgattga agtatgtgtg
     3301 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     3361 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     3421 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     3481 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     3541 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     3601 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     3661 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     3721 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     3781 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     3841 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcgac aaggcagaca acaagaaagg
     3901 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     3961 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     4021 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     4081 gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc ctattttccc
     4141 caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg tcgtcaaacg
     4201 acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg aaggggagct
     4261 gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct ttaaaagact
     4321 ttggatcaac tccttagaaa aagaagccac ttatcaggga ttccaacagt tacgagacgc
     4381 agaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     4441 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     4501 tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat tgcaggaaaa
     4561 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
     4621 tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag aagcatttga
     4681 agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     4741 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga tccttcagtc
     4801 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     4861 gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     4921 atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag ccgaagcgat
     4981 tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag tccaggaaca
     5041 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     5101 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     5161 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaggcaat
     5221 cggaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     5281 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtgga
     5341 tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag gtacgttgat
     5401 cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat ccatattgaa
     5461 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     5521 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     5581 ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga
     5641 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     5701 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     5761 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     5821 aggaatttgt ccaaaatgcc aggaacaaga agtcttgctc tatcaaaagg tggcagcttg
     5881 tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga aactcacagc
     5941 gacacagctg aaggagatca ttcaaaatag ccgaacaagc caaccagtga aagggttaaa
     6001 aggtcagaag ggttcatttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt
     6061 tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgccgca ctgcaaaata
     6121 atggagggtt aatcttatgc gtaataccac acacaaacaa caagaaatcg aaaatcaaaa
     6181 agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac tgcagcaatt
     6241 gaagaaccag cagaggattc aaaaagaaat cgaattagat cgataaaatc ttcctaactg
     6301 ttgtatacgt ataaacttat gttatactgt ttttataagt ttatacgtat acaaaggagg
     6361 gagaaaatga caaaagcaat ggtatacaag ctgctagaag aatttaatgg aactttggct
     6421 ttgaaggatg ccaaaaaagc gggcatttct cctgtaacga tcaaacgtat ggtagatcga
     6481 ggggagctgg atcgagagta tccaggtttt ttcaccttac ctggtcaatt tcctgatgaa
     6541 ttgtttatgg cgcaaaaaaa atatgaacgc ggcattattt cacatattac cgcgttagat
     6601 ttgtatgatt taacagatat gatccctaga cagatcgatc tgactgtacc ttacggttat
     6661 cacgtttctg aaaaaggatt gaaggaattc gcagttgagc tacactatac aaaagcggaa
     6721 tggtatgaat taggtaagat tgaaataaaa agtcgctatg ggaatcctat tatagcttat
     6781 gatacagaaa gaactttatg tgacatttgg aatccctggt ataccgttga ggatgaaatc
     6841 aaagtaaaag caatcaaaaa ctatatggag tcggacagaa aaaatcttag aaaattgaat
     6901 gagtatcgac gaattttacc aacagacaaa acgatgcgtt cttatattat ggctttaaat
     6961 taaaggagaa gatacatgac acctacgcag ttaaaagcaa aagtgaagaa tatttctaga
     7021 gaaaaagaag ttgatccgca gcttgtttta agacatttta tgatggaaaa attcttggaa
     7081 aaaatctcag agtctcctta tcgtgaaaat tttgtgttaa aaggcggctt cttgattggc
     7141 tcaaagtatg gcattgaaaa tcgcacaacc aaagatattg atacgacttt acaggaaatg
     7201 aaagtaacca aagaaacatt aacaacggtt ctaaacgaaa ttttttcaac gccgacgaaa
     7261 gaaggcattc aatttgagat tcaaggaatg aaagaaacga gagaaaccga ttactatcca
     7321 ggatttagct tacgtgtgct ggcacatttg gaaaacatgc ggccagattt taaggtagat
     7381 gtgactacag gtgactcgat ctatcctgca acgatcactc acagtcataa attgatgttt
     7441 gaggatcgaa cgattccttt ggaatcctat cctacagaac aaattattgc tgaaaaatta
     7501 agtgcaacct ttgatttttt aacagataat tcgagaggaa aagattttta tgatctctat
     7561 actattccta aaatggagaa gatcgatgag aagaccttga aagatagtgt gagaaataca
     7621 tttacacgtc gaggaaaaac tgatccgtta aagatatact atgaaaagga tatggatgta
     7681 ctgaaaaata gtcacgagct aaaagatacc tggaagaaat atcaaaaaag caatccttat
     7741 gctgcatcga tcacgtacga tgagacaatg gattcgatta gtaatttgat ggaaaaagtg
     7801 attcacgtcg aagaaaaaga gtaaaaacta gcttatcaaa gacaccaaca gatgaataga
     7861 gaaatggaga gataaagatg attaataatg tgacattagt aggacgctta accaaagatc
     7921 ctgatttacg ttataccgca agtgatactg cagtagcgac ttttacccta gccgtcaacc
     7981 gaaatttcac gaatcaaaac ggcaatcgag aagccgattt tattaactgc gtgatttggc
     8041 gcaaaccagc agaaacaatg gcgacattag ctaaaaaagg tagcttgatt ggtgtagttg
     8101 ggcggattca aacgcggact tacgacaacc agcaaggaca acgtgtatat gtgactgaag
     8161 ttgtagcaga taactttcaa ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg
     8221 ctgatcaatc gtccaccagt ccaagcaccg ccaccttcga acaacgggat acagcgacgc
     8281 ctaacaacaa tggattgaac gcttcacaga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttt tagtttgaga aagaaggagt aagcatggca aaaaaattag
     8401 tctgacgatg gatctattga tacccgtaat tgcatttata gacgggattt ctttgggcgt
     8461 cactgaaaat cctttggtaa atagaattgc gaaaggatca ctatttcttg tattcgtgta
     8521 tcttgtctgt tcttatcttt taaaaagagc aggaaaggat aattcctttt ggcgattgac
     8581 taaagtcaca gtagcagcga cagctgttat acttacggct attctaccgc tttatatgta
     8641 gagttaacgg gaaatcgttg aacttcttgc gaaatattgc tataatgaac atgaaaaaga
     8701 ggagttgcgc taacaacccc tcattaacag caccgattaa agaacggtgg cagaatatta
     8761 gttatttgga aataaccgct gacctgctaa agtagacggt tatttctttt tgtcattttt
     8821 aagcatattt acaatcaaac taatcaaagc aatggtaaac ataccaaaac caagaatggt
     8881 ttgaattgtc tcatatgcgg acaaaaggct tactcctttc atagatttta gcacttacat
     8941 tcataagcac caccaccttt ccagaagtcg ccaccgtctt caaactttac tgtcttgact
     9001 attatagcat atctaaaatc gaataaaaat tctcaatttt aactgttttt gctataattt
     9061 tagcgaggtg atcgtgatga acaaatatgt tggcttgctt gataaaatcc gagtaataaa
     9121 aacacaacca ttattggttc gctttacttt acaaacaatt catgaatcga ttaattgtgt
     9181 agttgcagat attgaaatta tcgataaatt gttaattatg gatgatggaa aatacaatat
     9241 tgccgtcaca ggtcatttca ataaacggaa tcaattagtc attgaatcga tgcaaattag
     9301 aaaccctgat cattttacgc gatcaatggg catataaata aaagaagagc tgcaatccta
     9361 atgtaggact gcagctcttt ttgttttaca tagaaacaag aaaaatgaaa aggccttttt
     9421 aggaaggcgc gagcgccttc tcaattgaaa aacctctctc actccaaccg ccaccgccgt
     9481 tcgagaagaa aggaagtgaa cattgcacgt tcattaagat aaagggtctt gcaaagcaag
     9541 acaaaataga aaaaacttcc tctcaccaaa aggttcgttc ctgttcgtta tctctttatt
     9601 gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag gacaagggta
     9661 aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gattcattct ggccaacaaa
     9721 tcaataaaga aagaactaac gaaaagaagt aaaccctttc ctaagaggag gcagaatcaa
     9781 tcatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc
     9841 atgcaccaca tactgtcgaa tgcgattgtg gacaaagagc gaaatgtatc aaaagttatc
     9901 gtttgtatca atgtccaaca tgcggaaaaa aatacacctt gtcttatggc gattatattt
     9961 tgattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
    10021 agaagtcatt tctgaaaaag tagcagaagc tttacttatt ccgcggtact ggacgataga
    10081 aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga agattgcagg
    10141 atatgaactg cattatcaag aaaacttgga aacttatgaa aacgaatatt ggttaaccaa
    10201 attagagaac taaaaaggag aaaaaaatat ggctcaaaat tactatgacg aatttgtcaa
    10261 attacctttg gataaaatgg cacaaaaaat ggaggatatg acgttcctat acaacgaaac
    10321 acgtgttcca aaaaaacatt ataaagaaaa attgtcggta gctgtggaag aaatgattga
    10381 atcaggtgtc gaaatgaatc ttattgcgac gtactatcga acactggaag aactgaaaaa
    10441 gcaaaatgca aaatggtttt tccaagcctt actatgcttg gaagtaggtg tcaaaccaag
    10501 cactattaaa ccttctgaat accaagcttt agaactaacg tatgcgaaat ttattgaaac
    10561 gaaaaaagcc aagactgttt caagtgaatg gttagattat tttgaaaata tcaataagta
    10621 tggcgcatat tacaccatga aaaaggaaga caatgaaaat gaataaggtg atttatcaac
    10681 taacaacgga ttacatatca acgatttgaa agaaaaaaag gtcaataatc atttatactg
    10741 aaaagtgaga atgtgtggaa taaggacaaa aaaagcctag taactaaggg aatggttctt
    10801 atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat aaacgggcaa
    10861 agtccgataa cttaagagaa agaaggtgat ccatttgaac aagattattc ccttattgtt
    10921 agtactgttt gttggcgaaa tacttatgcg agatagtgca gctgtacagt taaacctcgc
    10981 tgtatttttg gtaatactgg tgttttgttt gatcttttgg cggtttgtat tagtcctcgt
    11041 gagtctacta ttcaagatga ttgcagccat atttttcatg gggttatttc gatgaaagga
    11101 gagagtacca atgccaattg caataggaaa taaacggtta cccgtgacat tagatgaaaa
    11161 gagacaaaaa gaattgcagc aactaaagca gaagtacggc aaaagtgaat ccaggattat
    11221 gtgtattgcg ttagatttat tgattgccaa gaaaaagcag gatttgaggt accagcacta
    11281 agaaagtgac gtcacttttt atactaaaac ctaaaagtga tatcactttt agacaatgga
    11341 aaaggtggaa ataagatgaa gaaaaaacaa actaaactat cgttagcaga tattttaaca
    11401 cagttaaccg caactgaaga tggtgttgta gaatttgaac gatctgaaat ttcggtagta
    11461 gatgatcgtt actttaaaat gccttacttt tttgaccaag caaaggtcat ttgcctttgt
    11521 ggctacgatg gggtacgtga ttactttgga attagaatta ctgaagaaaa agtcgtttgg
    11581 gtaaacaatc atacagaatt aggtgcgcta gcctttgaag gaactgtatt ggacaatatc
    11641 tctattgtat tcgaggaaga aagctttacg cttgaatgcg ataagttaac ccgatatatc
    11701 gatccaaaat tctatgagga taaaaacctg gcatgggaac tggctttata gacagagtgc
    11761 tagtgcaagt aaaggaagaa aggaagatca ccatgaaata caatttatta gaccataagc
    11821 gaatgtgggt agagaaatta gaggaagact tagaacagtt ggaattgtta ggtcataata
    11881 agacttcaac ggtgtataaa gaaggactaa aacgaacgaa aaaagcacaa aaagaactag
    11941 caacact
//
LOCUS       DANKHK010000075        11750 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000075 DANKHK010000000
VERSION     DANKHK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(65..814)
                     /locus_tag="PW109_001948"
     CDS             complement(65..814)
                     /locus_tag="PW109_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511760.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(815..1141)
                     /locus_tag="PW109_001949"
     CDS             complement(815..1141)
                     /locus_tag="PW109_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2511761.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            complement(1289..1729)
                     /locus_tag="PW109_001950"
     CDS             complement(1289..1729)
                     /locus_tag="PW109_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2511762.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            complement(1810..3207)
                     /locus_tag="PW109_001951"
     CDS             complement(1810..3207)
                     /locus_tag="PW109_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2511763.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            3360..4631
                     /gene="celB"
                     /locus_tag="PW109_001952"
     CDS             3360..4631
                     /gene="celB"
                     /locus_tag="PW109_001952"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2511764.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            4758..5477
                     /locus_tag="PW109_001953"
     CDS             4758..5477
                     /locus_tag="PW109_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2511765.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            5855..6829
                     /locus_tag="PW109_001954"
     CDS             5855..6829
                     /locus_tag="PW109_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2511766.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            6882..7487
                     /locus_tag="PW109_001955"
     CDS             6882..7487
                     /locus_tag="PW109_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2511767.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(7581..8333)
                     /locus_tag="PW109_001956"
     CDS             complement(7581..8333)
                     /locus_tag="PW109_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2511768.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(8347..8607)
                     /locus_tag="PW109_001957"
     CDS             complement(8347..8607)
                     /locus_tag="PW109_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511769.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            complement(8629..9990)
                     /locus_tag="PW109_001958"
     CDS             complement(8629..9990)
                     /locus_tag="PW109_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2511770.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(10063..10362)
                     /locus_tag="PW109_001959"
     CDS             complement(10063..10362)
                     /locus_tag="PW109_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2511771.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            complement(10375..10833)
                     /locus_tag="PW109_001960"
     CDS             complement(10375..10833)
                     /locus_tag="PW109_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2511772.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITNYI"
     gene            11057..>11750
                     /locus_tag="PW109_001961"
     CDS             11057..>11750
                     /locus_tag="PW109_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2511773.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDN"
BASE COUNT         3577 a         2375 c         2029 g         3769 t
ORIGIN      
        1 aagcgtgtca cagtcttttt ttctacgccg gataaataga atatttttac aaatctgaat
       61 acattcacca gtaataagcg ctttgagtga attgacgata agaatgacgg actagactgc
      121 cataaatgat caagaagaca cccgttccta ccgtaacaaa aaatatagag gcaccgatac
      181 taccatccat ataccacatt aaagaagcca aaagtgggaa aagtgaaaaa atacataatc
      241 caattcctgc aatcagtgag aagccaaaac ttcgctgata ctcttcttct ttttcttttg
      301 cttctgctaa tactttttca ggtattttcc gatggtgcaa aaagtgattt aaacgtgtga
      361 ttttcatacc agaagcaata aaaaaaccga cagctaatgc tataccgaaa atcagacaag
      421 aaatacccat gaacacatat cctgctgaac aaaagaaaag aaaacttgca aaagataagc
      481 taatgaatag aacacctgat gccacttgga gacttgccgc tctgtattct ttccagtagt
      541 tttctgcttc gcttaaataa atagaagctg tgtttgtttc cgtctcgtca gcttgatgtt
      601 ttttttcgac atctagttct tctaataatt catcgattga accaaatgtc gaaattactg
      661 taccgatggc ctcctgttca ttttttcctt ctcccatcaa ttcatgataa tggtcttcca
      721 tatttgccaa caaatcagtt ttcagcttat ctatctcggt actttgaggt actccgataa
      781 aaagactgtc taaataatta cggattgtat ccatttaatt tccctccaaa aattgatcga
      841 ctacgtcttt cgtcgtcagc cactcttgct ttttttcttc taaaagatta cgccctattt
      901 cagtgatttt ataatatgtc cgcttttttc catgagtcac ttctccagga tacgaaacaa
      961 tcagttgatt cttttctaaa cgggcaaaaa cggagtaaag tgttgtctct ttgatcacat
     1021 aggaatcatt tgtcctctca cgaatcgttt tagaaatttc atatccataa gaatcgtttt
     1081 tgttaaggat cgataaaata atcgcatcat tgtatccgcg tattccatca cttgaaatca
     1141 ttttctttcc tccatattac tccatctgtc gttgtaatta gaatagcatg attacttcat
     1201 caggtaaagt aattttttac aaaaaaataa aaggaacaga aagcaaaaga agcgttatgc
     1261 ttttttgctt tctgttccct taatttattt attttacaac catgacgtta catgatgcgt
     1321 ggttgactac ataggaagta gtggaaccta ctagtgcacg ttctaccgca ttcagacctg
     1381 tcgcccccat gacaatcaga tcgattggtt ctttcgattc atggaaatta gcaatcagtg
     1441 tctttggata accgtaaaga acatatgtct cgatatctgt caggcctttt tcaattgcca
     1501 agtttcgatg ttctttcatc tggttttccg caacctcatc caattcttcc tgaaggcttg
     1561 ttgtaacaaa tgcaaaatca ccaaagtaat tgcctgttac ttcattgaca tacaggatgt
     1621 gcaacgttgc ctgatttcgt ttggcaatcg tgattgcctc aagaaatgct ttttctgcct
     1681 tttcagaacc atcaattgct actaaaatat tacgataagt atctaccatt gtaaacgcct
     1741 ccttcactgt tagtgtagca cgattacagt gttagaacaa acattttgtg aacgttttat
     1801 caaaaaatat caggcatcaa atccgttttt ttcagagacc gttttaaacc agcgacctga
     1861 ttttttgatc gtccgtttac cgtcttgtgc caaatcaaca gcgataaagc catagcgatt
     1921 cttataagca tttgtccaag accagttgtc catacatgtc cacatgtggt aacccacaca
     1981 attggttcct tcttggattg cttgatggac gtatttcaaa tgatccgtaa caaactcgat
     2041 ccggtaatca tcttcaataa ttccttcgct gttaacaaat cgttcttctc cttcgacccc
     2101 catcccattt tcagaaatat agcatttgat attaccatag ttttctcttg tattcgttaa
     2161 aatatcatag attccttttt cgtaaatttc ccatccacga tatggattca tttttttatt
     2221 gggcatgtcg taattatcaa aataatcatc tggcatgggg ctatgttcgt gattgattgg
     2281 agtttctttt gctttgattc gacgaggctg atagtaattg attcctagca ggtcaatcgt
     2341 gttttcacgg atgagttcta ggtctccttc atggatctct ggcattatag ccaattcttt
     2401 gacaatttcg atcaactctt gtggaaactc tcctttcact gcagggtcaa ggaatgatcg
     2461 gttgaagaat gcgtcagcga tcttcgccgc ttttacatct tctgggttcg tttcatctct
     2521 cggatagctt ggtgtcagat tcaagatgat tccgatctca ccatcatgtc cgccttcatg
     2581 gtaggctttg attacctttg cgcttgctag attctcatga tagcctactt ggaccgcttc
     2641 tttcatattg atatgatttg gatagtggaa ttgatacaag tagcctcctt ctacaggtac
     2701 gattggttca ttatgagtaa accatttttt gaccagatct ccaaacagtt caaagcacgc
     2761 tgctgcataa gaaacataag cgtcgaccgt ttctcttgat aaccatccgc ctttttcttg
     2821 cagtggtagt ggcatatcga aatggtaaag gttcacaaaa ggttcaatac cattatccag
     2881 caattcagta aaataatctc gataaaaaga aacagctttt tgattgattt cacctgtccc
     2941 ttctggaatc aaatgactcc attggataga tgtacgaaat gtgttatggc cagtttcttt
     3001 cattaactga acatcttctt tgtattttgt gtaaacttgc gaagtttttt caggtcctac
     3061 ttgatcgaag aatttttcag gctctaattc ataccaatga tcccaaatat tttctccttt
     3121 accatcatca cttactcttc cttctgtttg cggtccgcta gcagcagaac cccaccaaaa
     3181 attttctggg aatgtgtagg ttttcatttt tttacttcct ctcttttttc ttgataccgt
     3241 aatcataacg aaccctaaaa aataaatcaa tactttttat gaaaatgact agacatttaa
     3301 attttttggc gttatacttt gtatgtaatc gattgcaaat aaatttgagg tgaataaaaa
     3361 tgaacggttt tatggacaag ctttctgaga aaatcatgcc cttagctaat ttattagggc
     3421 aaaatcgtta ccttacagtg ctgcgggatg ctttcatgct ttccttccct ttaacgatgt
     3481 tcggttcgat cgttgtcgtt atcaataact tgcctttttt cagtgacgca acaaagggaa
     3541 ctttatcgaa tttattcggt aatgggcaaa atgcaacaat gagcatcatg tcggtttttg
     3601 ttacttttgg tatcggctat tatttatcaa aatcttatga cgttgaaggt attttcggtg
     3661 gtgccgtgtc ttttgccagc ttcttgattc ttacgccttt tgcaatgaaa accgctggcg
     3721 gtgaagatgt gactggtgtg ctttcacttg atcgtcttgg cgcaaaagga atgttcattg
     3781 gaatgatcgc tgctttcttg gctggagaaa tctactgcag aatcacgaaa cgcggctggc
     3841 agatcaaaat gcctgatgga gttccgcctg cagtaacaaa atcatttgcc gctttgatcc
     3901 ctgctgtagt gacactaact attttcttag taatcaatgc tgtgatgact ggtattttca
     3961 atgctaactt acatgacgtc gtttatgaag tgatccaaaa accattaact ggtttgggaa
     4021 gtagtttacc tgcaacattg attgctttgt tctttgtcca attcttatgg ttcttcggac
     4081 ttcacggaca aatcatcgtc aactctgtaa tggacccgat ttggaataca ctgatgcttg
     4141 ataacttaga agcttatcaa catggaaaag aattaccaca catcatcaca aaaccattca
     4201 tggaaacctt cactgttggt attggtggct ctggaatgac tttagctgtc gttttattga
     4261 tggcctttgt tcttaaaaag aaacagtatc gtgatgttgg acgattagct ttagcacctg
     4321 gtattttcaa tgtaaatgaa ccagcgatct tcggtctacc gatcgtattg aatgcaacaa
     4381 tccttattcc ttgggttgtg gcacctctaa tcgtaacgac gttcaattat ttggtaatgg
     4441 ctgctggact tgtccctgca ccaactggcg tctctgttcc atggactgtg ccaatcattg
     4501 caagcggtgt cttagcaaca aattcatggc ttggtggtat gctgcaagtc atcgactttg
     4561 tcatcgttgc ctttatctgg tacccattcc tgaaagtact agataaacaa ccagatttag
     4621 atattgctta atagatcatt aaggggttgt gacaaaaact tgtcatgacc tcttttttcg
     4681 aataaacaat gttccagatg ctacttcgag ttgtcggaag cataatcttt cgttatactc
     4741 tagaagaggt gaataacgtg ccaaaatacg aagaaatcgc aaatatttta agagaacgta
     4801 tccgttcaaa agagtatcct ctcgatagtt ttttgcctaa tcaagtcgac ttggtaaaag
     4861 aatttggtgc gagccggatg acaatcaaaa aagccatcaa tatcttagca atggaaggac
     4921 ttgtctattc gcagcgagga tcaggaacga aaatcttgaa tcatccattt atgcaaaaag
     4981 acacatcgcc cgctaacgag tatgaaggtt tgagtctcca aatgaaacgc agacatcgtt
     5041 ctttgaagag tcaaatcatt cattttgatg ttgaattccc taatcaatat atccaagaac
     5101 gattgatgat cgatgccgag caacctgtct acaatattca ccggttaagg atcttagatg
     5161 gcgagccata tgttttagag cataccttta tgccagtcca tcttgtgccg ggtttaaaaa
     5221 aagaacattt gttagcctct atctatgatc atcttcatca agaattgaat gtccattttg
     5281 caggggctta tcgtgctatt tctgcagata aaagtgatga atatgatcag caatatttga
     5341 actgtgcaga aaacgatcct gtactggaag ttcagcaagt cgtctatctc aaagatggac
     5401 agccgattga atattcttcc agccgaaacc gatttgatgt tcggaactat tctgtgctgg
     5461 atgtgcgttc aacttaacca aagaggttgt gaaaaagccg tttaccaaga gatcgtgaca
     5521 gtggtaagca gtttcaagta acaagaagag atcgtgaaat caacattcta ttctatccta
     5581 agcattagct caaaaatgaa aaaataactc agcataaaaa gacttatgac cacccgtaat
     5641 gattctaaag ttagcttttt aaagctagtt tttttgaaat cgatacagct agtcgtaagt
     5701 ctttttattt tattgatccg tccctcctct tttccattct tgattccttc ctgatacttt
     5761 agaagacaaa ttaattgcaa aatagagatg caccctctat actatgtcta aatcaactac
     5821 ttactttttg taagttaaca ggaggaaatt tatcatggat acacaaatca gaggaattca
     5881 ccacgtcaca gcaatgacat ctagcgctac aaaaatctac cgctttttta cagatatttt
     5941 aggaatgcgc ttaatcaaaa aaacagttaa ccaagatgac atcgaaactt accatcttta
     6001 ctttacagac gatactggtg cgccaggaac agatatgact ttctttgatt ttccaaatca
     6061 gccaaaagga aaaaaaggaa cagatacgat ctctcgtgct tctttccgtg tgccaagtga
     6121 cgcagcatta gacttttggt tggaacgttt tgaaaaatat gacgttactc attcagcaat
     6181 caatgaacgt ttcggcaaaa aatatatcga atttgaagat ttcgataatc agtattatca
     6241 attgatctct gacgaaaaca atcaaggtgt agcagctggc attccttgga aaaacagcaa
     6301 tgtaccgaca gagtttgcga tcacaggact tggacctgtc tttgtccgtg tcagtcaatt
     6361 agagcatatc cgcttgattc ttgaaaaaat cttaggattc aaagaaacag accattcagg
     6421 agatttctac ttatttgaaa tcggtgaagg gggaaatggt gcaagcatca ttattgaaca
     6481 tcggacagac ttgccaaaag caatcgaagg atttggaaat atccatcact tagcattgcg
     6541 tgtagcagac aaagaagcat tggaatactg gattcaagta atcaataaga ttgaattgcc
     6601 aaattctggt ttcgtcgatc gtttctattt ccaatctgag tacttcctag ccgctcatca
     6661 cgtactgttt gaattagcaa ctgatggtcc tggattcctt gaagatgaac cagcagaatc
     6721 agcaggagaa aaactatctc ttcctccatt cttagaagaa cgtcgtgaag ccattgaagc
     6781 ttatgtccgt cctttcgata caagtgatgc aaataaaaaa cgtgaataat ctcttcatgc
     6841 tacttcattt gcatctatag aaacagaaaa gagggaacaa aatgacctac atttttgaaa
     6901 aaggaacaag tggcaagcat ttgatcttac ttcatggcac aggtggcgat gaacattctt
     6961 tactggatat cgctcacttt cttgcaccaa acagtacatt gctttctttt cgtggcacgg
     7021 tccaagaaga tgggatgaat cgttttttca agcgtaatgg attgaatcaa ttcgacctgg
     7081 acagtttgga aaaagaaaca gataagctcc atgaaaaaat caaagaaatc agcgaaaaag
     7141 aactgatacc gttaagtgac tggctgcttg tcggctattc aaatggcgca aacatcgctg
     7201 ctcatttatt attggagcgt gagaccgagc taaataaagg gttgtttttc catccgatgt
     7261 ctctaggtgt tcatcaaagc cgttccacat tagaggataa aaccgtctgg atttcttatg
     7321 gtgataacga tccaatcgtt tcccctgctt cctttaaaga attggccgat caattcgaaa
     7381 cacgccaagc aaatgtgact attcagctaa ctgtaacagg tcatcagttg acgatggacg
     7441 aaatcaagca agcgagggaa tggacagacc acctcttaac taaataaaag aaaagaacta
     7501 cgacttacat aatacaacct gaagcagata tcgatctgga tcagattttg tgaaagtcgt
     7561 agttttttgt gtcagatagg ttagtctcca ccagctgcaa taacagattt attataaata
     7621 gccgttttat gtttcaactc ttccaatccc ccgattttct caccttttga ataaaaaccg
     7681 tcagtaatcc ctaatgcttg aaaaatcgtc tgcttgtagg caatttctgc attccattca
     7741 actgtatcaa ggatttgcag tgctttatgg atagcttcga ttcctgattc tccacctgtg
     7801 ttgatcaata cgccatgaga gttgagcagc aacatatgcg gtaattcgag atgctgtttt
     7861 tttacatagt cgcttgtcaa ttcagcaagt tcttcagagc aattaggctc aaagtccatg
     7921 acaggaatct cttttacttt agctgtggct tctgtcaaat taggcatagg caacccagta
     7981 gtcgcccaga acattgcatt aggcgcatga gcatgtagga cagctttgat agctggatta
     8041 gtctcataca tcgcttcatg catattgatt tcacgagtta aatgaccgac tccgtcaacg
     8101 attctacgcg ttcttggttc tactactaga acctgtgcag gagaaatctc tcctagatag
     8161 gcttctgaca tcatggtcgg cgtcatgatg atgtagtctt tcccttgatc atcagtggtt
     8221 ttaaaggaaa aattccctcc tgcgacattt gtgtttttcc tttcgaaaat cactcttacg
     8281 atacgtgcca tgtcctgtcg ttctctctca aataacatac gtccatctga cataatgacc
     8341 cctccattat ctttttataa aacttttttc tctcaatatt tctttggtag cttcttttat
     8401 tttcattgat tcttctattt tccgttttct tttttcatcg tactctctga ataaaacgtg
     8461 atttccatat ttatagatca ctagtgcgaa aaaaatgacc aatagattga tgatgatatt
     8521 ctgagtaaag agtccatata gaaatagcag aagtgtacca caaagagcga ctgtccattt
     8581 tattttatct gtctcttctc ttttcattac tttcccacca tttcaatgct atttttgtag
     8641 ttcagcataa cgttcactcg gtactttcgc ttttatcata tatttataaa gtagaacaaa
     8701 cagtcctaga aaaatcacgc atcctacaat agcgagaata ttcctattaa atgcctccgc
     8761 aaataaaacc ctgaactccg gcccttctat ggatgaccat gtgatcaact gtcctttctt
     8821 gattccctcg actgctccgc ttttttctgc taaatcactg agtattggcg caaggtaagt
     8881 ggccccgtac aaataaatag gcatagtgat gattcccaag atcagcatac gagccaagtt
     8941 tcctcctgtt aaaagtaatc cgccgaccgc aatcgaatag ttgataatcc ctgctaatgg
     9001 caatacttgg tttcctggca aaataatggc ataaccaata aacacaggaa caagtaaaat
     9061 agcagtaacc caaatttctg aacgaccagc taatatcggc cagtccaatc cgatatatac
     9121 ttcacgatcc ttaaaccgct ttttcataaa ttctgacata gcgtctgcta gaggagacaa
     9181 agattgcatg aacattttag agatcatagg aaacaaactt aatgctgtag ctgtttggat
     9241 cgctagatta agagaacctg aaacgccata cgcagcagca aagccaagcc ccaatccaat
     9301 aatgaatccc attacagagt tttcagagaa gatcccaatt ttcttcttca atgcattcgt
     9361 atccgctcgt ttatttaaag caggaatttt atccatgatc atattgaaag gcagcaacaa
     9421 gacagcggca gatgtcataa aatgagtcac tgttactaat ggaatccctg tgagatcttg
     9481 tatatgtttt tgaaacatat caccaagctt taattccaat ataacttgga ccattgcagt
     9541 aagaaagcct gcagccaaac taccggaaac ataataaact aaataagcag ttaaagcttt
     9601 cccccaaaca ttccacatat caacatttaa cgtcttcgtc caattaaaaa gtaacagtac
     9661 aaaattgacc ccaatcgtta ctgcaaaaaa tataaaagca taactccacg accatgtgat
     9721 gcttgctgca cctgtccacc caagatcaag tgcaggcaaa ctgattcccg tatttttagc
     9781 caatgcttct gatgctggac ttactgcgtt agacatatag ccaatgacca tagacattcc
     9841 gctaaaagca atccctaaag tcaaagcaga catgaatgct ttttttaatt tcatccctgc
     9901 caacaatcca agtatcaaaa taatcagggg aacaaaaatg gctgatccta agttcaaaac
     9961 ataattgata atggtttgga aaacttccat aaataaacct tctttctttc taaatatagt
    10021 aaataaaact atgaaccaac ttttttatcc aacaatcctc ctttactgta ctaactcttt
    10081 gatactgtca taaataggtt ctacacccat tccagttaaa aatggaattc ctggaaatac
    10141 ttcaataccc aaatcttttg cttgttcaag tacttcctca tcaggttgtg cgacatacac
    10201 atagattcca gcagttagaa gctcattttg aatcgatttg taatcaactg cttctacagg
    10261 aaatgcgatg ccgtcatctt ctaacatact ttggatctta ctagcgattg tctgactcgt
    10321 tgctactcca cttccacatg cgacaattaa ccgatttttc attttttctt cctcctaaat
    10381 ataatttgta ataagttcgt atatgttttg cggtgtttct tgtttcttgt aattttccac
    10441 gaatgtttca tccatgaata aattcataaa cgcttgaagt aatcctactt gttcttttgg
    10501 ttctttgatt cctaaaaaaa atagattttc tacagtcatt tcctgattat cccccatttg
    10561 ataaaaataa attgggttct tcagctgcat aacaaaaaca aatggctttt cgacatattc
    10621 acattccgaa tgtggcagcg cgatatttaa atacttagta atcaatccag taggaaattc
    10681 tctttctctt gcaatcagtc cattcaaata attctcattg acgtacccca ttttttctaa
    10741 ttgttgtgca gcgattaaaa aagcttcttc tttatttttt acttctaccg ctaattgaat
    10801 caaactagga acgaacatct ctttatattt cattccaacc tcctcctttt tgattaagtt
    10861 tgttaattta ttgttctact aagttcatct ttgttttaca agaacattat atttctgtta
    10921 gtttgttttg tcaacgcttt caaaagaaat ttattttgtt aaatttgatc tacttgaaaa
    10981 taaaatcgtt tatctatata attaagaaga acaaagtcga acaaaaattg acttttatgt
    11041 taaaggagta attcatatgc tgaaaaaaga aagacaagaa caaatcatta cattattaga
    11101 gaaagatttt tatttaacta attcagaaat cgctaacaaa ttaggcgtat cagaaatgac
    11161 catacgccga gatgtcactg aattagcaaa aaaaaataaa gtaaaaaagc tatatggtgg
    11221 cgttgaacga aaggaacctt ataatcgaga actatcgact caagaaaaga ttgatacgaa
    11281 tgtagccgaa aaaaaatata ttggtcaagt catgaacagt atgattcccg ataattctac
    11341 tatttatgtc ggtgctggaa caacgatcct atatgcactt cctttcattc aaaagaaaaa
    11401 tctatttgta attacaaaca gtttgattgc tttcaactat ttaaagaatc atacaagtta
    11461 tcggatttta cttactggtg gcgaattttc tcctattaca gaagagttca ttggggaagt
    11521 tgcagaaaga tcttttgaaa gtttgaatgt cgatatctct ttcgctgcaa caaacggcat
    11581 atttgataac aatgtgacaa ccgctcaatt tattgaggga aatattcaga acgcagcgct
    11641 caatgcagca aaaatcaaat gcattgttgc agattattca aaattaaata aatcagacgt
    11701 ctatactttt agaaaattaa ccgattttga ttatctaata accgataatc
//
LOCUS       DANKHK010000076        11549 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000076 DANKHK010000000
VERSION     DANKHK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(144..629)
                     /locus_tag="PW109_001962"
     CDS             complement(144..629)
                     /locus_tag="PW109_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2511774.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    719..1064
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1109..1750
                     /locus_tag="PW109_001963"
     CDS             1109..1750
                     /locus_tag="PW109_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2511775.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1769..2404
                     /locus_tag="PW109_001964"
     CDS             1769..2404
                     /locus_tag="PW109_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2511776.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2422..3237
                     /locus_tag="PW109_001965"
     CDS             2422..3237
                     /locus_tag="PW109_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2511777.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3556..4992
                     /locus_tag="PW109_001966"
     CDS             3556..4992
                     /locus_tag="PW109_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2511778.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5181..7628
                     /locus_tag="PW109_001967"
     CDS             5181..7628
                     /locus_tag="PW109_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2511779.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7858..8925
                     /gene="rlmN"
                     /locus_tag="PW109_001968"
     CDS             7858..8925
                     /gene="rlmN"
                     /locus_tag="PW109_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2511780.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9118..10221)
                     /locus_tag="PW109_001969"
     CDS             complement(9118..10221)
                     /locus_tag="PW109_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2511781.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10774..11322
                     /gene="aac(6')"
                     /locus_tag="PW109_001970"
     CDS             10774..11322
                     /gene="aac(6')"
                     /locus_tag="PW109_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2511782.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3832 a         1951 c         2495 g         3271 t
ORIGIN      
        1 aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga
       61 ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg
      121 gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa
      181 acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga
      241 tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc
      301 tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca
      361 ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt
      421 tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt
      481 gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact
      541 gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca
      601 taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta
      661 tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca
      721 aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa
      781 agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg
      841 aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt
      901 ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg
      961 atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag
     1021 gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga
     1081 tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt
     1141 attagtagga gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc
     1201 attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct
     1261 tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt
     1321 ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt
     1381 tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc
     1441 tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa
     1501 aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa
     1561 cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg
     1621 gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta
     1681 tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta
     1741 ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt
     1801 tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc
     1861 tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga
     1921 aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa
     1981 aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa
     2041 tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata
     2101 caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta
     2161 tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat
     2221 gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca
     2281 gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac
     2341 ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca
     2401 ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt
     2461 cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc
     2521 aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga
     2581 gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa
     2641 tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac
     2701 aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa
     2761 gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca
     2821 ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct
     2881 ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat
     2941 gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat
     3001 ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa
     3061 gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag
     3121 tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt
     3181 gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa
     3241 ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc
     3301 tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt
     3361 tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc
     3421 acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct ttacatattt
     3481 ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag
     3541 gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg
     3601 ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa
     3661 tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat
     3721 ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt
     3781 ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa
     3841 gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat
     3901 attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat
     3961 aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt
     4021 gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact
     4081 tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat
     4141 gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta
     4201 gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt
     4261 aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg
     4321 gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga
     4381 tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt
     4441 cttgggatca attactatca aacggcgacg aatgcattca atccattaaa cggagttggt
     4501 gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga
     4561 atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac
     4621 ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg
     4681 attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac
     4741 gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat
     4801 ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac
     4861 ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct
     4921 ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga
     4981 gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt
     5041 tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa
     5101 agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt
     5161 gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga
     5221 cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt
     5281 ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga
     5341 ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag
     5401 ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac
     5461 gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat
     5521 ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca
     5581 gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc
     5641 tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat
     5701 tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta
     5761 tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc
     5821 gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc
     5881 gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg
     5941 tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg
     6001 agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag
     6061 agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt
     6121 tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag
     6181 actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg
     6241 agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg
     6301 tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc
     6361 cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt
     6421 tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg
     6481 aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa
     6541 accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa
     6601 cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt
     6661 cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa
     6721 aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc
     6781 tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac
     6841 aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt
     6901 acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat
     6961 tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg
     7021 aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag
     7081 ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca
     7141 gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg
     7201 cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga
     7261 tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta
     7321 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
     7381 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
     7441 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
     7501 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
     7561 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
     7621 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
     7681 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
     7741 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
     7801 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
     7861 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
     7921 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
     7981 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
     8041 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
     8101 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
     8161 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
     8221 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
     8281 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     8341 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     8401 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     8461 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     8521 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     8581 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     8641 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     8701 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     8761 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     8821 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     8881 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     8941 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     9001 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     9061 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     9121 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     9181 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     9241 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     9301 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     9361 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     9421 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     9481 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     9541 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     9601 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     9661 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     9721 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     9781 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     9841 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
     9901 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
     9961 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
    10021 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
    10081 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
    10141 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
    10201 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
    10261 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
    10321 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
    10381 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
    10441 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
    10501 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
    10561 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
    10621 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
    10681 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
    10741 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
    10801 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
    10861 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
    10921 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
    10981 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
    11041 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
    11101 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
    11161 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
    11221 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
    11281 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
    11341 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
    11401 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
    11461 aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt
    11521 gcataaattt ggcttaatac cgagaaact
//
LOCUS       DANKHK010000077        11501 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000077 DANKHK010000000
VERSION     DANKHK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..202)
                     /locus_tag="PW109_001971"
     CDS             complement(<1..202)
                     /locus_tag="PW109_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2511783.1"
                     /translation="MMKYLQIVVIDGMNCSRCASKVRNALEAEPTVSVKIVDLARGTV
                     TFDADSKLTLTFLNQLLKNTKYT"
     gene            349..1490
                     /locus_tag="PW109_001972"
     CDS             join(349..577,577..1490)
                     /locus_tag="PW109_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2511784.1"
                     /translation="MAKAKKKFDEDFKKTILDLNQSGQSVEELAEQYGIATQTIYRWK
                     KLHTKNEATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGESLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSMN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDHALLHQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAHLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            complement(2233..2442)
                     /locus_tag="PW109_001973"
     CDS             complement(2233..2442)
                     /locus_tag="PW109_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2511785.1"
                     /translation="MTEHLQPLFISGMACLGCAGEIKYALEKNPSISVRKVNLEEGLV
                     TIATKTDLSISVINQLLKNTNYKAL"
     gene            complement(2459..4909)
                     /locus_tag="PW109_001974"
                     /pseudo
     CDS             complement(2459..4909)
                     /locus_tag="PW109_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628114.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(4893..5258)
                     /locus_tag="PW109_001975"
     CDS             complement(4893..5258)
                     /locus_tag="PW109_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDL2511786.1"
                     /translation="MVLVEHKIQVSNSELDVLKFIWRYEPVTCGLITHGMQERNSWHP
                     STTKTLIRRLLDKNVITFNTSKNQRYYTSLIKKQEFLETEIQRLLSGMDEGCISEVSY
                     YLNGLVKRSDTEEFNDFKI"
     gene            complement(5274..5888)
                     /locus_tag="PW109_001976"
     CDS             complement(5274..5888)
                     /locus_tag="PW109_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2511787.1"
                     /translation="MKKSMLFLLFFVFALSLAITLTINFPGLLKINLFFQSDINFQNW
                     SRSQVNQDFRNLMGYLNNPFQKELVFDNLYVSDRGINHFKDVKFLFQLNYSLLLSTSS
                     VLLYLNRKKLVTRDQVREITSLIKWMIISVCVMALLFFDKAFVLFHQVFFDNDDWMFD
                     YRTDPIISFLPETFFFLCFLLIVTISVSTLTTIHHLFNKEERTL"
     gene            6044..6847
                     /locus_tag="PW109_001977"
     CDS             6044..6847
                     /locus_tag="PW109_001977"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2511788.1"
                     /translation="MREPYDRVFISFGPFTIYWYAIFIVFGIIIGYFVANRRAKRAAL
                     PEDTIVDLLLYGLPVSIISARLYYVLFELPLYIDDPLSILKIWEGGLAIHGGLIGAVL
                     TGVIYCKKKNLSFWNVADVVAPSIVIGQIIGRWGNFMNQEAYGEIVDIEFLQSLKLPS
                     FIIDQMYIDGAYHHPTFLYESLWNIGVLIILLLVSRNRMFFGQIFLIYVSLYSVGRFW
                     IEGLRTDSLMLTANLRMAQVLSIVLLIGSILTYIYLKKSKEEDLHGSIT"
     gene            6831..7055
                     /locus_tag="PW109_001978"
     CDS             6831..7055
                     /locus_tag="PW109_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HDL2511789.1"
                     /translation="MEALLNLLPLLLLLICPISMMFMHKGHGHAGHHHMHGEDHSKHT
                     GSENDIASLKIQTEQLKEEVATLKNLVKER"
     gene            7058..7237
                     /locus_tag="PW109_001979"
     CDS             7058..7237
                     /locus_tag="PW109_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HDL2511790.1"
                     /translation="MQWLLLLLCPIMMIFMMRGMHSGSHNEKDDEQLKQDLENLKEQN
                     DILSSELSDLKSRLK"
     gene            complement(7351..8901)
                     /locus_tag="PW109_001980"
     CDS             complement(7351..8901)
                     /locus_tag="PW109_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HDL2511791.1"
                     /translation="MYTNYNMNQLTLDLSTSFSPKENHVAVFIHELVEALQINDPYLF
                     GRPREYDLGAMMKVVLFAYTRETFTSRKIERLAEENLYARWLTQERVPTYRTIARFCV
                     SNDVQELTNKGLDQLTEYLRARNLIDDALFIDGTKILADANKYSFVWKKNTIRFDQMN
                     REKLLTMMTELKEAYALKQIPEGTTLTVDMIDELLTRMELRLDELENEVEATKRLSPN
                     PAKQQRRTLKSQVRKLNAKREKLLEHQTQFSIYGTRNSYSKTDHDATFMRVKEDHMKN
                     GQLKPAYNLQIATCNQFVLGYDVFQNPTDTKTLKPLLEKMKTAQKSPHYLVADAGYGS
                     ESNYRYIEDELPQHTALIPYGTMFKEQSKKWQTDDRKVMNWVYEPNEDFYIDPKGVRF
                     NFHAYRQRTDADGFVRDFKEYQAEKTDANQALIPEALTPKGNRRKITVNPSWEYHKEK
                     QKERLSTPDIQKIYGRRKVDVETVFGFMQACSGFTRYTVRGLEKVRKQTGLLITAINM
                     MKLTKIGT"
     gene            complement(9612..10895)
                     /locus_tag="PW109_001981"
     CDS             complement(9612..10895)
                     /locus_tag="PW109_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDL2511792.1"
                     /translation="MVGVITVDNTYLTDVFNQYKLRENLLYQTWFIKSEKRLKAFKKV
                     RKYVQVLIKHVNNGTFPTDFKNSALEDIMNVIAEQTEIFKGAKHAFMWKPKLRIPDIY
                     ENRENQLAFSLCLDQILKANQEIKMLFAVNILAEKKIRGLGPAVANILYFLEPTIFPP
                     FNTAIVNGYNYLTGSKIRLGKWEDYFKLRDGMIELNCLGGLFSKDLGAISAFLFDIGK
                     LNYIVRENEEEYVRISESNTQKLIKQKLEKEDGKNVHQKIQYILATLGNEIGYHAWIA
                     SNDHNRIVDGNRLGDVSIPLLPETIYQLPNPLSRTIGLIDVIWFSKNGEPICAFEVEK
                     STSIISGMNRMDDLYSIHQENLMMYIVSPDQREEEIRAQFNRPHYMRNQNLHDHLRYI
                     LFTDLEKHANFMKKFGKDFTILAPISHYPSGELVR"
     gene            complement(11074..11481)
                     /locus_tag="PW109_001982"
     CDS             complement(11074..11481)
                     /locus_tag="PW109_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2511793.1"
                     /translation="MNKTLRLMKPWDYFIITAVIMASFVPYLLFDTNNDENNNRESTD
                     VIAVVKIDGEVVDKFNLSQVNESFEKTYYPQKGKYNIIEVAPGKIRVKEDNSPDQIAV
                     RTGWISRPGEMSICLPHKLIVEILGVEENPLIY"
BASE COUNT         3583 a         2162 c         1913 g         3843 t
ORIGIN      
        1 cagtatactt tgtatttttt agtagttgat ttaagaatgt taaagttaat tttgaatcag
       61 catcgaaagt aactgtacct ctagccaaat caactatttt tacactgact gttggctctg
      121 cttccaaagc atttcttact tttgatgcac accttgagca attcattccg tcaataacca
      181 ctatttgcaa atatttcatc ataaaattcc ttctttctta aaaaagtcgc ctatattgga
      241 tttactaatc taatcaatct tttaagaatt gtcttacaag tgatgttagg atgatttcat
      301 ggacacgatt tggtagaatc taactactaa gaaaaggacg tgtccatgat ggcaaaagcc
      361 aagaaaaaat ttgatgaaga ctttaagaag acgatcttag atttaaacca gtctggccaa
      421 tcggtcgagg aactggcaga gcaatatggt atcgctacac aaacgattta tcgttggaag
      481 aagcttcata caaagaatga agcaaccggc atgactgagg cagaaatcct agcaatgaaa
      541 aaggaaatgg cccgcatgca ggaggaaaac accatcctaa aaaaggcttt aaccatattc
      601 gctcaaaagt aaaaatgagt gatgtcttcg aatttattca atcaaatgtt catgatcatg
      661 acgtaaaaca aatgtgtacc gttttggaag caccgaaatc cagctactat gactggaaga
      721 aaaagaagcc ttccaagcgc caatcagaaa acaaacgctt aaaccgcttg atttcaggaa
      781 tctactttga aaacaagggg atctatggtg cgcccaaaat tcataagatt cttgttggcc
      841 gtggcgagag cttgtcccta aagaaagtac aaatactgat gagaaaatta ggcttgcgtt
      901 cagtcaccct caagaagtac aagcctggca aacaagcgaa agttaaaagt gaaggacgta
      961 aaaacttgtt aaaccaagat tttaccacta cgtcgatgaa tcaaaaatgg gtgacagata
     1021 ttacctatat tcatacccta aaagatggct ggacctacct ctccactatc caagacctcc
     1081 acacaaagaa agtcatcggt tggaagtttg gcaagcagat gacgaaagaa ttggttatcg
     1141 aaacccttga ccatgcgctc ttacaccaaa caccctccga gaacttgatc atccactcag
     1201 atttgggttc tcagtacact agcgaagcct atgaggcaaa gctgaatgaa ttgaacattc
     1261 ggcactcctt cagtcgtaag ggctgtccct acgataatgc cggcatcgaa tcgtttcacg
     1321 cctcactcaa aaaagaagaa gtctatccat caaaagcgta tgaaaacttt gaagctgctc
     1381 atttagcgtt atttcaatac attgaaggat tttacaatcg caagcgaatc catagtagta
     1441 tcaactattt aacaccaaat cagatggagg agttggcatt gcaagcttag ttcctagcta
     1501 ccacctacat ttttttggcg agatttccac agggcttcaa cttcatcgtt tcgatcagcg
     1561 gtcaaacggt tttggtttga cggctggtcg aaacgatgag atactcaccc gctggaatct
     1621 cgaaaaaatt ctcataaatc gtgtccgagg tattgactca aatccaaagt atcataagga
     1681 aaatttatgg agaaaccatg tatagagttt gttttagagc ttttaaatat aggatagata
     1741 cagattgatt tgtttagtgt atgattgcat atcatgtaca tatatcgcta agtatcgcgc
     1801 ccaaaacata ggtttcgagt ttcatgaata tcctaacagg tagaagtgct atgatgatat
     1861 tcgataagca tcctaatatg aaatataagt ttagaactgc tattttggat agttcttcct
     1921 cctattcaaa taaataattg ttctacataa gaatttcaca atcctctcta ccctcctcta
     1981 ctaccaagtg aaaatccatt tcaactaata ccaaaaacct acattgggat tccaaaggat
     2041 ttttgagttc aaaaaaacat gaaatgcctc tcagaaatcc gtccattgga ttcagcatat
     2101 acttatgcac aaaaagcagt gaataaattt aaaaaactgg tttttctata tatctcaatt
     2161 tgaaaaaagt agaatataga ggaactttct taccataaga attcacatat ttaatgtgat
     2221 gattcaccaa acttataatg ccttataatt cgtatttttc aacaattgat taataacgct
     2281 aattgataaa tctgtctttg ttgcaatagt tactaaacct tcttcgagat ttactttcct
     2341 aacagaaatt gagggattct tttctaatgc atacttaatt tctccggcgc atcctagaca
     2401 agccattcca ctaataaata agggctgtaa atgttcagtc ataagacaat cacctttcct
     2461 atttgacttt gaagtttttt aagcgaagtg aatttaaaag tactgttatt gagctaaaac
     2521 tcatagcaac tcctgcaatt aacgggctta ataaaggcct ccaaaaaatg ttcaaaacac
     2581 ccatcgctat tggaacactt aacatgttat aagcaaatgc ccagtacaaa tttgacttta
     2641 tattccaaat tgtttttttg cttaaatcaa tcgtggacat tagcaattgc agatcgtcgt
     2701 tcatcaatat tatatccgca gattccatcg ctatatcagt accagaaccc atcacaaaac
     2761 ctatatccgc ttgagcgagg gtaagtgcat cgtttatccc attacctacc attgcaaccc
     2821 tatgttcttt tgactgcaac attttaacat agtaggcctt ttcttgaggt aatacctcac
     2881 taataacgtt ggtgatccca acttgattag ctattgcctg tgcagtaagt ttattatcac
     2941 ccgtgagcat aaccacattg aggccaagtt tattcaactt ttgaattgct aatgcacttg
     3001 ctggctttat agtgtcttca atcgcaatta ttccgagaaa gatacgatca tttgcaatat
     3061 acactggtgt ctttccttta gatactaaat tatctgaaat taacttaaat ttactaatgt
     3121 ctatattcaa ctgcttaaag aactcttcat ttcctaaact tacttcggta cctttcaata
     3181 tagcattaac gcccaatcaa gagaaagatt cgaatatact aggttcaacc agatcaatcc
     3241 ccacgatttc ggcttctttt acaattgcct ccccaagcgg atgctcggat gctttttcag
     3301 cagaggccgc taaaaacaag agaaagtttc tatccatatt ttctgcaagt attatatctg
     3361 ttaccacagg ttttcctttt gtaatcgtac cagttttatc gaataatatt gtttttacgt
     3421 gatgagttgt ttctaaagct tcaccacttt taattaagat accattttca gcgccttttc
     3481 ccataccgac cataatagct gtccgtgtag ctaaaccgaa tgcacaagga catgttataa
     3541 ctaaaactga aattaagact gagaatgaaa acgaaatact ttgtccagat agcacccaaa
     3601 gtagactaga tccaatcgca actacaataa tgatatatac aaagtaactt gaaatgatat
     3661 cagccagttt ttcgattgga gctttcgaag attgtgcatc atccactagt ttgcttattt
     3721 gagctaaagt ggtgtcttta ccaatatgag ttgtttcata tcttattacg ccatttttat
     3781 ttttactggg tcctattact cggtcattag ctttcttttc aacaggaaga ttttcacctg
     3841 taggcataga ttcatcaact aaagacttac cacttattac aataccatcc agtggcaaac
     3901 gctctcctgg ttttgctatg atcacatctc ctactaaaac tgattcaata ggaacagtct
     3961 gttccttatt atgtctgata gttttcgctg ttttgggagt catcctcatt aatttttgta
     4021 ttgcttcaga tgtcttatct ttcgtcacca actctaggta tttctctagt ctgattaacg
     4081 ttaatatcac tgctgatgct ccaaagtaga gctcttttgt atagagataa tttcccaata
     4141 taatctgtat tgttgcaaag atgccgtata acaaagcaat acttgtacct aaagcaatta
     4201 atgtgtccat tttcggatgt tttttcatga gtgaggaaaa tccaatttta taatagtctt
     4261 tattggccaa tataaccgga gcggtaagta ttaattgcgt catcgaaaaa aaactagggt
     4321 ttgtcgtagg atcaattatt tcaaataggg gtaactcaag tatatttgca ctagcaacat
     4381 atataatagg aattgtaaat agcattgacg tgataaatct tcgccttagc ttctgttgct
     4441 gttgctcttg ttcttttgtt ttatatatta cttgttcctc atcttcagaa agagcatata
     4501 gctgatatcc aagaattttg acaacatcac ttatttttgc aaccgctgaa gggtcagttg
     4561 atattaccga gagtttttct gcttcaaaat ttacgttggc atatttgaca taagtcattg
     4621 atttaactac attttcaatt ttgttagcgc aggacacaca agtcattcca cttattccaa
     4681 atacctttat ctcagttttt tttcgaatct cagcgtcata accaatgtct tgaatagctg
     4741 ttataactgt atgattagac aaaacacctt catcatattc caattttaga acctcagttg
     4801 ttaagttaac tttagcgaaa tatactccat ccaactcatg aatttttttc tctattgttg
     4861 ttgcgcaaac tgcacaagtc attccataaa gattatattt tgaaatcatt gaattcctcc
     4921 gtgtcagaac gtttaaccaa tccgtttaaa tagtagctta cttctgagat acaaccttca
     4981 tccattccag aaagtaacct ttgtatttcg gtttctaaaa attcttgctt cttaatcagt
     5041 gaagtgtagt atctttggtt ttttgaagtg ttaaacgtga tcacattttt atctaacaac
     5101 cttcttatta acgtttttgt tgtggaggga tgccaactat tacgctcttg cattccatgt
     5161 gttattaaac cacatgttac cggttcatat ctccagataa actttaaaac atctaactct
     5221 gaattcgata cctgtatttt gtgttcgacc aagaccatcc ctccttaggc tattcataaa
     5281 gtacgttctt ctttattaaa caaatgatga attgttgtca aagtagaaac acttatagtt
     5341 acaattaata aaaagcataa aaagaaaaat gtttccggta aaaaactgat aattggatct
     5401 gtacgatagt caaacatcca atcatcattg tcaaaaaata cttgatgaaa aagcacaaat
     5461 gctttatcaa aaaacagaag tgccattaca cagactgata ttatcatcca cttgattaaa
     5521 ctagtgattt ctctgacttg atcacgcgtt actaattttt tcctgtttag gtataaaaga
     5581 acgctactcg tagaaagaag caatgaatag ttaagctgga acaaaaattt aacatcttta
     5641 aaatggttta taccgcgatc tgagacgtat agattatcaa aaaccaattc tttttgaaaa
     5701 ggattattta aatagcccat taaatttcta aaatcttgat taacttgaga tctactccag
     5761 ttttgaaaat ttatatcgct ttgaaagaat aaattgattt tgagcaaacc agggaagttg
     5821 attgtcaatg taatcgcgag ggataaagca aagacaaaaa ataatagaaa tagcatgctt
     5881 tttttcatta aattaatatt tatattagat cactcctttt aatataatga taaaaaaact
     5941 tgtgtagaaa atgtgtacca atgtatctgt caaaaagtct acataaaatt tccataatag
     6001 cctgctatag tcaaaacaag ttcattagga aggagggaag aatttgagag aaccctacga
     6061 cagagtattt atttcgtttg gtccatttac tatttattgg tatgccattt tcatagtgtt
     6121 tgggatcata atcggatatt ttgttgctaa tagaagagca aagcgtgcgg cattgcctga
     6181 ggatacaatt gttgatctgt tgttatatgg attgccagtt tctattatat cggccagatt
     6241 atactatgta ctttttgaat tacctttgta cattgatgat cctttaagta tcttaaaaat
     6301 ttgggaaggt ggtctagcaa tacatggtgg attaattggt gctgtattga caggggttat
     6361 ttattgcaag aagaaaaatc tttctttttg gaatgttgct gacgtagttg ctcccagtat
     6421 agtaattgga caaatcattg gacgatgggg aaattttatg aatcaagagg cctatggaga
     6481 aattgtagac atagagttcc tgcaatctct gaaactccca tctttcataa ttgatcaaat
     6541 gtatattgat ggggcctacc atcatccgac ttttttatat gagtctttat ggaatatagg
     6601 agtactcatt attttgcttc tggtgtctag aaatagaatg ttctttgggc aaatattttt
     6661 aatttatgtt tcactatatt ccgtcgggag attctggatt gaaggattac ggaccgacag
     6721 tttaatgcta acagcaaact tgagaatggc tcaagtcctg tccatagtcc tattaattgg
     6781 ctctatatta acttacatct atctcaaaaa atctaaggag gaagatttac atggaagcat
     6841 tacttaatct cttaccacta ctattactac taatttgccc tatctcaatg atgtttatgc
     6901 ataaagggca cggacatgct gggcatcatc atatgcatgg agaagatcat tcaaaacaca
     6961 ctggttccga aaatgatata gcaagtttaa aaatccaaac tgagcaatta aaagaagaag
     7021 ttgctactct aaagaactta gttaaggagc gatgacaatg cagtggttat tacttttatt
     7081 atgtccgata atgatgatat ttatgatgag gggtatgcac tcaggatcgc ataatgaaaa
     7141 agatgacgaa caattgaaac aggatcttga aaatttaaaa gagcaaaatg atatcttaag
     7201 tagtgaacta agcgatttga agtcaaggtt aaaataagtt aaaggaggcg tgacataagt
     7261 tacctagtta cgctctccaa aaaacaagga gcacttggaa atcatgagat ttttcgagtg
     7321 cttcttgttt ttttattcat tttgagtagt tcaagttcct atttttgtca atttcatcat
     7381 attgattgcc gtaatgagta aacccgtctg ttttctgact ttctctaagc ctcgaaccgt
     7441 gtagcgagtg aagcccgaac aagcctgcat aaatccgaag actgtttcca cgtcgacttt
     7501 tctccgccca tatatttttt gaatgtcagg cgttgataac cgttcttttt gtttttcttt
     7561 atgatactcc caagacggat ttacagtgat ctttctacga ttcccctttg gggttaaggc
     7621 ttcaggaatg agcgcttggt tcgcatccgt tttttctgct tggtattctt taaaatctcg
     7681 gacaaagcca tccgcatctg ttcgttgacg gtaggcatga aaattgaatc gtaccccttt
     7741 ggggtcgatg taaaagtcct cattgggctc gtaaacccaa ttcattactt tccggtcatc
     7801 tgtctgccat ttcttacttt gttctttaaa catcgttcca taaggaatca gtgcagtatg
     7861 ctgaggaagt tcatcttcta tatagcggta attgctttct gaaccataac ctgcatcggc
     7921 gaccaaataa tgaggggact tttgtgcagt cttcattttt tccagtaatg gtttcagtgt
     7981 tttagtgtct gttgggtttt gaaagacatc gtagccgagc acaaattgat tacatgtagc
     8041 gatctgcagg ttatacgcag gtttaagttg gccatttttc atgtggtctt cttttacgcg
     8101 cataaatgtc gcatcatgat cggtttttga ataactgttt ctagtcccat aaatagaaaa
     8161 ctgcgtttga tgttccagaa gtttttctcg ctttgcattt agtttgcgta cttggctttt
     8221 caacgttcgc cgttgttgtt tcgctggatt tggcgaaaga cgtttcgttg cctcaacttc
     8281 attttctagt tcatccagac gcagttccat ccgtgttaac agttcatcta tcatgtctac
     8341 ggtcaaggtc gtcccttcgg gaatctgttt cagggcatat gcttctttta gttccgtcat
     8401 catagtaagc aatttttcgc gattcatttg atcgaaccgg atcgtatttt tcttccagac
     8461 aaagctgtat ttattcgcat ccgccaagat tttggtcccg tcaataaaca acgcatcatc
     8521 aatcagattc cgtgcacgca gatactctgt caactgatct aaacctttat tggtcaactc
     8581 ctgtacatcg ttggatacac aaaaacgggc gatagttcga taagtcggaa cccgttcttg
     8641 cgtcaaccag cgagcatata ggttttcttc agctaagcgt tcaatcttgc gacttgtaaa
     8701 ggtttctcgc gtatacgcaa aaagaaccac cttcatcatg gcacctaaat catattctct
     8761 tggtcgacca aaaagatagg ggtcattaat ctgaagcgct tcgaccagtt catggataaa
     8821 aacggcgaca tgattttctt ttggagaaaa tgaagtagat agatccaaag tgagttgatt
     8881 catgttataa ttagtataca ttttgtttag tcctttcagg attaaatgat ggtgcattgg
     8941 cgggaaccgg tgctcctttt tattttaccc aaaaaaccgc aagaaatgaa cgctttctcg
     9001 tcatttcttg cggttttctt ctatggactt atgtcacagt ccctttaaaa ttcactaaat
     9061 tcatggttca gaatagaatt gattctctca aaatatttag tctaacttta ctctttataa
     9121 aacgaataag gcatgcgtgg tttataatct ttctttatgt atttctgaca ccaaagaaaa
     9181 tgatcctcat cattccatcc actcgttgca ttccaccaag agtacataac atcccctggt
     9241 gtatagggta gtccatacac caggggattc tagaagtaag atttattgtt tcttttttaa
     9301 gtgcaaataa aaccattatc ttcgatgaat ataaggaaaa taccttttta gataacattt
     9361 ctaggttatg aatagatagt attcactcta tccgcaattt tcttcaacga aaagttacta
     9421 aaatggaagc atgggcagca ttaatatgat gattttcttc tggacgttgc taagacattc
     9481 aatagcaatg agcgccatta taaatgtgga agctactcgt aaaaaaaggg aagattgatt
     9541 tccaattctg cccaaatgct tatttatact acaattaacc gtattaattt caacttcttt
     9601 gtccagaata actaccgaac tagttcacca cttggataat gagatatcgg ggctaaaatt
     9661 gtaaaatctt tgccaaattt cttcataaaa ttagcatgtt tctctaaatc tgtaaataga
     9721 atgtatcgta agtgatcatg caaattttga ttacgcatat aatgtggtct attaaattgt
     9781 gctcgtatct cctcttcacg ttgatccggt gagacaatat acatcatcaa attttcttga
     9841 tgaatcgaat acaaatcatc cattcgattc atcccagaaa ttattgacgt cgatttttca
     9901 acctcaaaag cacatatcgg ttccccattt tttgaaaacc aaataacatc aatcaaacca
     9961 atcgtcctac ttaacggatt tggtaattga tagattgttt ctggtaatag aggtatagac
    10021 acatctccta agcgattccc atctacaatt ctattatgat cgtttgaggc aatccaagca
    10081 tgataaccta tctcattacc taaagtagct aagatatatt ggattttttg gtgcacattt
    10141 ttgccatcct ctttttccaa cttctgtttt attaatttct gggtgttact ttctgaaatt
    10201 ctgacatact cttcttcatt ttcgcgaaca atataattta gtttcccaat atcaaataaa
    10261 aaagctgaga tagcacccaa atctttcgaa aaaagtcctc ctaagcagtt gagttcaatc
    10321 ataccatcac ggagtttaaa atagtcctcc catttaccta agcgaatttt cgagcccgta
    10381 agataattat atccatttac aatagctgtg ttaaaaggag ggaaaatggt aggttctaga
    10441 aaatataaaa tgtttgcgac agctggacca agccctctga ttttcttttc tgctagaata
    10501 tttacagcaa ataacatttt aatttcttga ttagctttaa gaatttgatc aagacaaagt
    10561 gaaaaagcta actgattttc cctattttca taaatatccg gaattctcaa tttaggtttc
    10621 cacataaatg catgcttggc tcctttaaaa atttctgttt gttctgcaat tacattcatt
    10681 atgtcctcta gggctgaatt tttaaaatct gttgggaacg ttccgttatt gacatgtttg
    10741 ataagtactt gtacgtattt cctaactttt ttaaacgctt tcaaacgttt ttcacttttg
    10801 ataaaccaag tttgatacag taaattttcg cgtaatttat attgattaaa cacatctgtt
    10861 aaataagtat tatccactgt aatgacacct accatcccta ttaatatttt ataaactcgt
    10921 tagctctaat atggctttct atatgcaaaa ttccgtaacc accttaaatg aagaataatg
    10981 taaacaggtt ttagcgattt tagttaacgg tctttcctta gattactccg tccatgtctc
    11041 tataatagga ttcgtggtac ttattagttc atactaataa attaaaggat tctcctctac
    11101 tcctaaaatt tcaacaatca atttatgagg cagacatata ctcatttccc ctggtctact
    11161 aatccagcct gtacgaacag caatttgatc aggactatta tcttctttta ccctgatttt
    11221 tccaggagct acttcaataa tattgtattt ccctttttga gggtaatatg ttttctcaaa
    11281 agactcatta acttgtgata agttaaattt gtctacaact tctccatcta ttttaacaac
    11341 tgcaattaca tctgtacttt ccctattatt attttcatca ttattagtat caaatagtaa
    11401 atatggtacg aatgatgcca taatgaccgc agtaataatg aaatagtccc aaggtttcat
    11461 gagtctcaat gttttattca ctaaattcgt tctcctgtat t
//
LOCUS       DANKHK010000078        11473 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000078 DANKHK010000000
VERSION     DANKHK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            159..1418
                     /gene="lysA"
                     /locus_tag="PW109_001983"
     CDS             159..1418
                     /gene="lysA"
                     /locus_tag="PW109_001983"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2511794.1"
                     /translation="MTELIIGGVTANTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1405..2205
                     /gene="dapF"
                     /locus_tag="PW109_001984"
     CDS             1405..2205
                     /gene="dapF"
                     /locus_tag="PW109_001984"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2511795.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2208..3089
                     /locus_tag="PW109_001985"
     CDS             2208..3089
                     /locus_tag="PW109_001985"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2511796.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGKEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3086..3802
                     /locus_tag="PW109_001986"
     CDS             3086..3802
                     /locus_tag="PW109_001986"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2511797.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3808..4503
                     /gene="dapD"
                     /locus_tag="PW109_001987"
     CDS             3808..4503
                     /gene="dapD"
                     /locus_tag="PW109_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2511798.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAGTVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4519..5520
                     /locus_tag="PW109_001988"
     CDS             4519..5520
                     /locus_tag="PW109_001988"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2511799.1"
                     /translation="MREIDTCIVGATGLVGQTILKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5517..6878
                     /locus_tag="PW109_001989"
     CDS             5517..6878
                     /locus_tag="PW109_001989"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2511800.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLSLQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYSKMIVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6838..7989
                     /locus_tag="PW109_001990"
     CDS             6838..7989
                     /locus_tag="PW109_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2511801.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFHYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSRSKEERVYNDEFIQSI"
     gene            7964..9130
                     /locus_tag="PW109_001991"
     CDS             7964..9130
                     /locus_tag="PW109_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2511802.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPKHVKQAGIAAIEEDFSHYTGMRGLEELREAACMFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFVQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTE"
     gene            9309..10688
                     /locus_tag="PW109_001992"
     CDS             9309..10688
                     /locus_tag="PW109_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2511803.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSVSDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11092..11473
                     /locus_tag="PW109_001993"
                     /pseudo
     CDS             11092..11473
                     /locus_tag="PW109_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3832 a         2007 c         2538 g         3096 t
ORIGIN      
        1 gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat actagacaat
       61 tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa atgaaattcc
      121 aataaaaacg gaaaaaaaat gaaaagagga atgaaaaaat gacagaattg ataattggcg
      181 gagtcacagc aaacacattg gcagaaaagt ataagacacc tctgtatgtg tatgatgaag
      241 aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa cgttttgata
      301 caaaagtact ttacgcctct aaagcattcc aaacgattga gatgctgcat ttagttcgtg
      361 aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct ttgcaagctg
      421 atttccctag agatactatc tacttccatg gtaacaacaa gacgccagag gaactgatct
      481 atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa gttgaattat
      541 tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg
      601 gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca aaattcggca
      661 tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa agcagctatc
      721 tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt actgcttggt
      781 tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct ctttccttga
      841 atcttggcgg agggtttggg attcgttata cacaaaatga tcagccaatc ccagtcgaag
      901 aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga aaactgacca
      961 ttcgtcaatt actgattgaa ccgggtagaa gtattgttgg agaagctggt acaacactat
     1021 acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta gatggcggga
     1081 tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat ttagcagcaa
     1141 aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg
     1201 atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt ttagctgttt
     1261 attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg acacgaccag
     1321 cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa gcattttctg
     1381 atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg cggaaatgat
     1441 tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc ttctttatgc
     1501 acaaaagaaa aatatgatac agacggattg atcgccgtaa aaatcgatcc gttggaaatg
     1561 atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt
     1621 gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt agagacatta
     1681 gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag cgtagatatg
     1741 ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac
     1801 caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt aggaacgatt
     1861 cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg
     1921 atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga agtagtcaat
     1981 gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact tgcttgcggt
     2041 acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc agcagatcag
     2101 gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc gatacaaatg
     2161 gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg ataaaaggtt
     2221 cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat gctggtttgg
     2281 aaaaattgat ccaattccat ttagatgaac aaacagacgg attattagtt ttaggtacaa
     2341 caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa ctgacagtaa
     2401 agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat acgaaagaaa
     2461 cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg gtcatcaccc
     2521 cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca attgcagaag
     2581 cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg tcgattccaa
     2641 tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa gaagcctcag
     2701 gagatatggc ttatgtgatg gatgccgctc ggttgattgg aaaagaattc ttcctttaca
     2761 gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg
     2821 tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg caggacggaa
     2881 gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat gccttgttca
     2941 gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta ccttccggac
     3001 ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta gctcagcttt
     3061 tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg gacagatggg
     3121 aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt tacggacggc
     3181 acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta ttgatttttc
     3241 tcatccagat aatttagaga aaatcctttc gtatagcttg tcttatcaac ttccgttagt
     3301 cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac atgccaaaca
     3361 tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga atcaactaat
     3421 caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag
     3481 tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa tccaagaagt
     3541 gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa accaaattgg
     3601 tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt ttgctgctac
     3661 tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg ctgcaggcgc
     3721 agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat tagaagattt
     3781 attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa
     3841 acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt
     3901 aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga ctatgagacg
     3961 attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct
     4021 gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg tatcgaacca
     4081 ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg
     4141 attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg tgctgtctta
     4201 ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt cttagcaggt
     4261 gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact ggtaggtgcg
     4321 aatgcagtga ttgttgaagg tgtccatatt ggaaaagatg cagtcgtggc agcaggagca
     4381 gtggttttag aagatgtggc agcaggaact gttgtcggtg gaatacctgc acgtgtatta
     4441 aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt acgcgaatta
     4501 tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc aaccggatta
     4561 gtcggccaaa cgattctcaa agtgttagaa gaatatgatt ttccagtagg acgattgaaa
     4621 ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact
     4681 gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt
     4741 gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt tgtcatagat
     4801 aattcctcag catggcggga agattcggat attccattaa tcgttccaga ggtcaatctg
     4861 gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat tcaagcggta
     4921 cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta ttcgacctat
     4981 caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac
     5041 gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga aatcgatcag
     5101 cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac ccgtaagatt
     5161 ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat cgaaaatgga
     5221 catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga aattagagag
     5281 tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca taaatatccg
     5341 acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag aaaagacact
     5401 tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa aggagccgct
     5461 gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt ggcaagatga
     5521 gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa gagcaattca
     5581 aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt
     5641 taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg atttatgctt
     5701 accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat cgtttcattc
     5761 aagtaagaga tgttctttcc ctgcagttac ctattgaaga tgaactagca cgcatacaga
     5821 aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt gaatatctga
     5881 ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa gacctgattt
     5941 tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc acaaaagcat
     6001 ttaaaagata ttcgaaaatg attgttccag ggttttacgg ggcgaaccct gcaggaaaag
     6061 aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg aagatgttgc
     6121 aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct gatccgcgaa
     6181 tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga gagatggctt
     6241 atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag gctgatatcc
     6301 ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg
     6361 aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt ctttccatca
     6421 ctttattcaa acgacatatg tccgatgaaa tcgggtttat ctggaaagca atgagcattt
     6481 ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat atcggagtga
     6541 tcgtctcagc agaagcgatt gccgataaat tatttctcat cacgaaagaa ttaaaagaaa
     6601 cacttggcgt ggaagaaatc gtcgtgattg aagacttagc tttgatctct gtcgttggcg
     6661 gaccacataa agaattgatc ggtttatctg gaaaagtgtt atcgatattg aacaaattag
     6721 aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc atcggtgtac
     6781 cgaataacca atatgaaaca gtagtgaagg gtatctatga agggatggtg aatacggatg
     6841 ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcggatttca tgaattcaag
     6901 acaaaaaaat atttatatga acaagtaaaa aacagcgggg ccgttatcca tgaaatcggc
     6961 gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc ttttcgaaca
     7021 gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc taagcatgaa
     7081 gggtatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg gttgactgac
     7141 tttataacta aacacattga ggatcttatt tataatgtag tactgatcta tcagccatca
     7201 gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga taaatatgaa
     7261 gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga agttttctct
     7321 cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa aggccagtct
     7381 gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt acgatttttg
     7441 aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct aatgaaattt
     7501 ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat tgtatctgga
     7561 agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt agaccattta
     7621 gcagaacagt ttcactctac agctactatt tcttttcact ataatgacgg ctatccagct
     7681 gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact acatgaatta
     7741 gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata tccatgtgta
     7801 tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt cgattttgat
     7861 atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac agaaaatctt
     7921 cttgtaaaaa tcgatagcag atcaaaagag gagcgagtat ataatgatga atttatccaa
     7981 tcgatttagt cccaggcttt ctagaataga agtatcaaaa atacgacaat tcgatcagca
     8041 aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt ttccaactcc
     8101 taaacatgtc aaacaagccg gaatagcagc gattgaagaa gacttttctc actataccgg
     8161 aatgagagga ttagaggaac tgcgggaagc ggcttgtatg tttcagcaac agcgttatgg
     8221 attaacttat gatccgcaaa cggaagtttt gacgacagtt ggcgcaacgg aagcgattgc
     8281 ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag cgcctgctta
     8341 ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga tagatacttc
     8401 tgatacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac aatatggatc
     8461 agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga cattgtctgc
     8521 aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg ttataagtga
     8581 tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta cttatcttcc
     8641 agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg gttggcgagt
     8701 gggctttatt tttgttcaaa aagcccttat tgacgaacta attaaagtcc atcaatatct
     8761 ggtgactgcc gctacaacga tgtcgcaaaa agctgcagct gaagcgctaa taaatggggt
     8821 gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc tggtccagca
     8881 attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact tgttttgcaa
     8941 actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag cggaacaggc
     9001 aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt atatccggat
     9061 ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca aagacttttt
     9121 aacagaatag tgattcatag attttatcaa aaaaacaatc gttaattttg cggtatattc
     9181 tgcaaaatta acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9241 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9301 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9361 caatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9421 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9481 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9541 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9601 tatcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9661 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9721 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9781 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9841 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9901 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9961 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
    10021 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
    10081 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
    10141 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10201 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10261 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10321 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10381 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10441 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10501 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10561 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10621 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10681 tagaataaga aaacgaaata tttgaaaaga ggttgtgaca gaagtggtca gcttcaagaa
    10741 ataagaagga attcacgaaa atcgtttctc aaatttttgt gaaatttcgg cttatttttc
    10801 gaagaagtta cttctggaac accgtgagtc agaggttgtg aaaaagctgc cttgcatcaa
    10861 gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggct ggacttatta
    10921 ccgcagatgc aagcttttga acaccgttta ttttcaaaaa agggactgtg acacgatttg
    10981 tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg tattttttat
    11041 ggttaaatat acttttgaac ttaaattaaa gatcgttcat gattatttgg atggcaaagg
    11101 cgattggacc gtaaaaatcc agatcaagag attgaagcgg aaatgcttaa aattcgggag
    11161 aagcacaagg attatggctg tttacggatg accaatgaac taagaaaccg cggtttctct
    11221 atcaataaaa agaaagttca gcgcctgatc aagaagctag gaataaaagt aacggcatac
    11281 acacctaaat cacggcgcta caattcttat cgaggacaag tcggagcagt agcccaaaat
    11341 cggatccatc gtcgtttcta caccagtatc tgccatcaga aaatcacgac ggatacaact
    11401 gaatttaaat accatgaagt ggatgccaaa ggcattatcc gtcaaaagaa attgtatcta
    11461 gatcctttta tgg
//
LOCUS       DANKHK010000079        11195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000079 DANKHK010000000
VERSION     DANKHK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            163..1119
                     /locus_tag="PW109_001994"
     CDS             163..1119
                     /locus_tag="PW109_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2511804.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1209..2312
                     /locus_tag="PW109_001995"
     CDS             1209..2312
                     /locus_tag="PW109_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2511805.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2325..3278
                     /gene="menA"
                     /locus_tag="PW109_001996"
     CDS             2325..3278
                     /gene="menA"
                     /locus_tag="PW109_001996"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2511806.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4213..5559)
                     /gene="gor"
                     /locus_tag="PW109_001997"
     CDS             complement(4213..5559)
                     /gene="gor"
                     /locus_tag="PW109_001997"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2511807.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5693..6946)
                     /locus_tag="PW109_001998"
     CDS             complement(5693..6946)
                     /locus_tag="PW109_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2511808.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6952..7875)
                     /locus_tag="PW109_001999"
     CDS             complement(6952..7875)
                     /locus_tag="PW109_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2511809.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8096..10588)
                     /locus_tag="PW109_002000"
     CDS             complement(8096..10588)
                     /locus_tag="PW109_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2511810.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10595..11062)
                     /locus_tag="PW109_002001"
     CDS             complement(10595..11062)
                     /locus_tag="PW109_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2511811.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3302 a         2277 c         1965 g         3651 t
ORIGIN      
        1 ttttggattt ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg ctgaaagaaa
       61 acgtttgaaa tattacatgg gttatttatc aaaaatagtt gacttatagg ctaaaaagat
      121 tagaatgtat aatgaatcac aaagaaaaat tgtggactag aaatggaatg gtggggtatt
      181 aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat tagcattttc agctctagtg
      241 ttaggcgctt gcggcgcaga tgacaacgga agctcaaact cttcttctga atcatctaca
      301 gcacaatctt cgactgcaaa aagcacaaca gaatcatctg caaaagttgt agctggcgga
      361 gatttgcaag acggcacata taaattagaa gaaaaaaatt attcaaatgg ttaccgtgca
      421 gtctttgaaa tggttgtaaa agacggcaaa atcactgaat caaaatatga taacgtcaat
      481 gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca aaaacatgga agcaaaatct
      541 ggcacaaacc cagaaaaata cattcctgag ttgaatgacc aattcttaaa agcacaaagc
      601 gcaagcggtg tggaagtcgt aactggagct actcactcat ctgaaagctt ccaaaactac
      661 gcacaacaat tgatccaagc tgctcaagct ggtaacacag atacaatcga aatcgacaat
      721 ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa agaactactc taacggctac
      781 cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa ctttgattat
      841 atcgacaaag acggtaaatc aaaacaagat gatacagaat ataatgaaaa tatgaaagca
      901 aaaagcggta cagaaccaaa aacttacatc cctactctga acgacgaatt cgtcaaagca
      961 atgggcgaag aagatggctc acctgctaat gtagaagttg ttacaggtgc tacacacagc
     1021 tcacattcat tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa aggcgacact
     1081 caaacaatcg aagtagataa tatcgtaaca gaaaaataat cacataatca tactaataaa
     1141 agttggaggg tgggacgata gccagattag gcagtcgtct cacctttcaa aatgaagaga
     1201 ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac actgggcatc tcttttatgg
     1261 tgctaactgg ctgcagcaac tcagcaaata aaacaaccac tgaaagttca gcagctacca
     1321 agatattgaa agatccatat tctgatgaac agttcttgtt agggacttac gttcgtatcc
     1381 gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga gtaaaagaac
     1441 ttggtgataa aatcacgatc aatcaaaaag gttctgagat cgatgaagtg aatgagcaag
     1501 ctgggatcaa accagtaaaa gtttctgatg acgtctacac gttagtcaag cgggcctacg
     1561 aatatagcca agattctcaa ggcggattcg atatggcgat tggtgcaatc acacaattat
     1621 ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga agaaatcgat caggcattga
     1681 aattagtgga ttatcataag atcgaactaa atgacaaaga aaaaactgtt tatctaaaag
     1741 aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg gacgaagtag
     1801 tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc ggtaatgtct
     1861 atgtcttagg ccacagccca agaggcgaaa accaagactg gacagtcggc atccaagatc
     1921 cgaacatggc tcgcggatct gttttaggtt ctattaaaga acggaacaaa acattagtta
     1981 cctctggaat ttacgagcgt tatttagaag tagatggcaa aaaataccat catttgtttg
     2041 atccaaaaac gggttatccg ttcgacaatg atatcgcaag tgtcacgatc ataacagata
     2101 aatcaattga tggtgacggg ttgtctacag cagtcttttc aatgggtgtg aaaaaaggac
     2161 tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg acaaaagacg
     2221 ataaagtata tgtgaccgat ccaatcaaag acaccttcaa attatcggag gactcccatt
     2281 acacgatggg tgaccgttct gaactaaaat aaggagtacg aaccatgaca ctaaaaatct
     2341 ttttagaagt agttgaaata cgaacaaagg tggctagtgt atttcctttt gcggtagggg
     2401 tattattctc tatcgctttt tttcatgaga tacattggct aaacactttt ttatttttta
     2461 ttggaatgct agtttttgat ttagcgacta ccgctatcaa taattacatg gattatgaaa
     2521 aggctcattc tgaagtatat aagtatgaag aaaatgtcat tggccgcaga aatctatctc
     2581 ctgtattgat tcgaaacatg attttagcta tgattgcatt tgtttttgtt atcggcgtta
     2641 ttctaacgat caatacgggt tggcttttac ttttgatggg atttatctgt tgttttattg
     2701 gagtattcta tacattcgga ccgattcctt tatctcgaat gcctttaggg gaaattttca
     2761 gtggggtgac tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat acattcggtt
     2821 cagatgtcta tgatctgaca gtcaactttt catctggaca atttgctttg gtaggaaatc
     2881 tttgggctat agcagcaatc gtattagctt ccttaccctt ggtgtttacg attgccaata
     2941 tcatgttggc taataatttg cgggatcttg accgtgatat tgaaaatcat cgctatacac
     3001 tagtttttta tatcggacgt cctgcgggaa cagtcttatt ccagctattg atgtatgctt
     3061 gctatttcgt gattttaatg gggttgttga gcggtatcta tgaatggccg atcttggtca
     3121 ccttcttgac cttgccaatt gtgaagaagc atttggatca attcaagcaa acgttacccc
     3181 agcctatcag tttcagctat tccatcaaaa atatggtctt attcaatgcc agttatgtat
     3241 tggggctatt actaacaatc attatgcaag ccatttaaca gcatgaagag gttgtgaaaa
     3301 aactgccctg catcaagtaa taagaacagc caaataaaaa tagctcctta tatttttcgt
     3361 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttatcag gaggttgtga
     3421 aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt
     3481 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgtg
     3541 aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt
     3601 tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt
     3661 aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt
     3721 tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag
     3781 tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc
     3841 agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc
     3901 gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat
     3961 acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa
     4021 tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta
     4081 tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga
     4141 aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact
     4201 gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac
     4261 tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag
     4321 catctcatcg acaccaacac caatagcatg taagccgacg attctttctt cttttccgac
     4381 acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt
     4441 aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc
     4501 tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata
     4561 gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc
     4621 gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc
     4681 atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt
     4741 tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca
     4801 atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt
     4861 aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat
     4921 caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca
     4981 catgacttca ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc
     5041 acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat
     5101 tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat
     5161 tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc
     5221 gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag
     5281 aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc
     5341 tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg
     5401 ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc
     5461 ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc
     5521 agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga
     5581 gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt
     5641 cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac
     5701 gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca
     5761 ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt
     5821 gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt
     5881 ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa
     5941 atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat
     6001 tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt
     6061 cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga
     6121 catttgacac cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt
     6181 ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca
     6241 tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag
     6301 gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat
     6361 cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac
     6421 catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca
     6481 ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg
     6541 tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg
     6601 tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac
     6661 gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag
     6721 cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt
     6781 cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca atatatactg
     6841 catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga
     6901 tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat
     6961 agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt
     7021 actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg
     7081 tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa
     7141 tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag
     7201 attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg
     7261 tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc
     7321 ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt
     7381 agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc
     7441 ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt
     7501 gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc
     7561 aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt
     7621 tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat
     7681 cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg
     7741 ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata
     7801 tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt
     7861 tatgagttcg atcataattt tctcctccta caacttcata atagtagcat agctctaaaa
     7921 actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca
     7981 aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca
     8041 caacctctta atcaagtctt actattataa aaattcagct acacaggcca agtaactata
     8101 gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag
     8161 cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc
     8221 gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa
     8281 atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt
     8341 ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt
     8401 tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag
     8461 ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt
     8521 ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca
     8581 taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat
     8641 catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta
     8701 agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat
     8761 accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt
     8821 ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt
     8881 tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac
     8941 gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag
     9001 cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc
     9061 tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa
     9121 catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa
     9181 ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa
     9241 agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga
     9301 tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat
     9361 cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat
     9421 tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg
     9481 tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc
     9541 gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg
     9601 tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag
     9661 catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata
     9721 agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt
     9781 caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt
     9841 cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg
     9901 ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa
     9961 gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt
    10021 cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg
    10081 tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat
    10141 caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta
    10201 agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat
    10261 gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa
    10321 cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac
    10381 cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac
    10441 gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg
    10501 aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt
    10561 gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac
    10621 gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca
    10681 atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt
    10741 cttcataaag tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata
    10801 actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta
    10861 tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg
    10921 tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc
    10981 ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat
    11041 ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat
    11101 aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc
    11161 aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DANKHK010000080        10864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000080 DANKHK010000000
VERSION     DANKHK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(181..795)
                     /locus_tag="PW109_002002"
     CDS             complement(181..795)
                     /locus_tag="PW109_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2511812.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1401..1778)
                     /locus_tag="PW109_002003"
     CDS             complement(1401..1778)
                     /locus_tag="PW109_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511813.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2078..2959)
                     /gene="galU"
                     /locus_tag="PW109_002004"
     CDS             complement(2078..2959)
                     /gene="galU"
                     /locus_tag="PW109_002004"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2511814.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            3369..4619
                     /locus_tag="PW109_002005"
     CDS             3369..4619
                     /locus_tag="PW109_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2511815.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(4761..5756)
                     /locus_tag="PW109_002006"
     CDS             complement(4761..5756)
                     /locus_tag="PW109_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2511816.1"
                     /translation="MYSDIKIVKGNGGYGGPLIIRPTKVKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETSDNKETVNTTSPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(5774..6328)
                     /gene="srlA"
                     /locus_tag="PW109_002007"
     CDS             complement(5774..6328)
                     /gene="srlA"
                     /locus_tag="PW109_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2511817.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWIGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(6316..6807)
                     /locus_tag="PW109_002008"
     CDS             complement(6316..6807)
                     /locus_tag="PW109_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL2511818.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHGQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPTLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            complement(6800..8666)
                     /locus_tag="PW109_002009"
                     /pseudo
     CDS             complement(6800..8666)
                     /locus_tag="PW109_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(8685..9485)
                     /locus_tag="PW109_002010"
     CDS             complement(8685..9485)
                     /locus_tag="PW109_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2511819.1"
                     /translation="MPDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            9709..9924
                     /locus_tag="PW109_002011"
     CDS             9709..9924
                     /locus_tag="PW109_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2511820.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            10063..10548
                     /locus_tag="PW109_002012"
     CDS             10063..10548
                     /locus_tag="PW109_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511821.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVTSFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT         3243 a         2189 c         1750 g         3682 t
ORIGIN      
        1 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
       61 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      121 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      181 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      241 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      301 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      361 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      421 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      481 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      541 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      601 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      661 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      721 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      781 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      841 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      901 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
      961 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1021 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1081 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1141 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1201 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1261 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1321 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1381 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1441 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1501 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1561 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1621 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1681 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1741 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1801 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1861 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
     1921 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     1981 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2041 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2101 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2161 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2221 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2281 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2341 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2401 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2461 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2521 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2581 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2641 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2701 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2761 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2821 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     2881 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     2941 ctgctttttt gaccttcact tgctgttcct cctcgtaaaa tatcatttgt ctaatagttt
     3001 ttattcaaaa tgtttctaga ttataaaatt tattgaataa cacattccta acggaatttg
     3061 tttcgatatt gtgtgatttc tcagagtaca atggatgtat ggaaaggatc caacgggaat
     3121 ttgctatttc ctcctgtagc gtttgtctac cctgtttagt ctgttacccg aaccactgtt
     3181 atcactacca actcattagc tgtttcgata atggactaga ctttcaaata gtagtttgga
     3241 aggcagcttc tagctctttt atcaccgtat tctattaggc gaggttgtgg ctatacagtg
     3301 agtttctggg aaatcccaca gtatcgaagc attttccaaa atttatatcg aaaggaggtc
     3361 cttcttccat gaaattattt gtcggtattg acgttagctc tgaaaaacta gatgtttgtt
     3421 ttttaacaga cgacaatcaa ttgtctatct tatctgaaat ctctgttgcc aatgacatcg
     3481 aaggtgcttc attcatacga gaaacaattc ttgaattcaa taacagctac cactttgatc
     3541 aaattgtgat tggcatggag tcaacgtcca tgtacagctt ccatccttcc atgttttttc
     3601 atgaagatga gaaactaaag aagctcaaca cacttgtaac gattgaaaat ccttttcggg
     3661 tgaaacaatt tagtcgcatg tttgatgaga ataaaactga tcgaaacgat gcattaagaa
     3721 tcgctgattt cttgcgtatt caacgattca caacttcccc tatcaaagaa gagaaataca
     3781 tggccttaca gcgtttaaca cgtactcgct atcaacttat taagcaattg attcgtacaa
     3841 agcaacattt tcttgaaaat ctaacgtaca aatgtaatac gcttgctcga gaaatgcgag
     3901 acgaatcgac gagtcttttt agtgcaaccc tcatttcact catgacggaa gatttcacat
     3961 tggatgaact tgctgagctt cctttagaag ctttctgtga tttacttcaa gaaaaaggaa
     4021 aaggccgttt caagcaacca gaaaaaatcg ctaaagcaat tcaacgtgca attaccatga
     4081 gttaccgcct aggtgctctt gcacaagaat caattaatgt tgttttaagt gttctcgtac
     4141 gagaaatacg agcacttgaa aaaaatatca aagaattaga taaagccatc gaacaagtaa
     4201 ttgttgtcat tccagaatat caatgcttaa ccagtattcc aggcgttggt aaagtctatg
     4261 ctgccggttt aatcgctgaa attggtcaaa tcgaaagatt tgaagatcag acaaaattgg
     4321 caaaatacgc tggattaagt tggaaagtga accaatctgg aaactatcag tcgcaaaata
     4381 cacctctgac aaaacaagga aatcgatatt ttagatacta cttagttgaa gctgccaact
     4441 ctgtaaaaaa ttatcttcct gaatacaaag ctttttatca aagtaaatat aaggaggttc
     4501 caaagcatca acacaaacgt gcactcgtcc taaccgcaag aaaatttacg cgtctggtgg
     4561 atacgctact acgtaaacac caactctata cgccaccaag gagtgtgata gagaaataac
     4621 aatccgttat tgacgcaaat ccttgaatca acccgaaaaa aatttgattt tgatcgggtc
     4681 tagtttcgtg cgcttcaaaa catattactc aataaagaaa atcttaaatt tcatcttgac
     4741 ttatcaccat tagactaaac tcagctatat aatccaaaac ttgctaccca agcaatgata
     4801 actcgtggga tgccgttaag aaatcttgaa taaagaactg atggtacacc tacttctact
     4861 gtctcagctt ttgcttcttc caaacctaga ccgactggaa taaaatcaca tgcattttga
     4921 gtattgatag caaatagtgc cggtaatgct aattggggag gaatatttcc ttttccaatc
     4981 tctacaccga tcaatgttcc aatcacttgg ctaataactg cccctggacc aagcaaagga
     5041 gataaaaatg gcagtgaaca aataaagcca atgattgtta atccaactgc atttcctgct
     5101 aaaggtgcca taatgttagc aaatacatta ccgatcccag aaccttgtat cacaccaatc
     5161 aaaagggaaa cgaaagccat aaaagggatc actgtccgga tcatcgtatc tactgcttct
     5221 cttgctgatt gattgaatgt agcaactact tttcccgcgc ccattccaat ttttgctatc
     5281 cagttttgct gctctgcttt ttgctcggtt atctttttag aagtatcata tttgtacttt
     5341 ttatctggag atgtagtatt tactgtttct ttgttatcac tagtttccat tagtggctgt
     5401 atttgttcta atcctactgc tgaaacataa atatcttcag tgatatattg tgctaatggg
     5461 ccactttttc ctgtgggaac gatattgatg gttggtatcc cttttttagg ataaattcca
     5521 catctaagag tccctccaca gtcaacgatt gcaagcgcga tttcactatc aggtattgat
     5581 gttttgaatc cattgataga ctccatacct gtcagttctt ctattttatc tacgatcatt
     5641 ggcctctcac ctccacctgt gacatagatg aacttgtgtt taacttttgt cggcctaata
     5701 atcaaaggac ctccataccc gccgtttcct tttacgatct tgatatcact atacatttaa
     5761 aacactcctt tctctaatca actgaaagct ctactttttt gctaagaacg attccttgct
     5821 gtctgcatac aaatccagta gtaaaatcag ttatccaacc accaataaag ttcatcagca
     5881 gtcccacaag caagtatcgg atagccagct ccatcgaatt tagccctaag gtttcgatcc
     5941 cttgagcaat cccaatccat acaaacaatt ctccaggatt aatatggggg aatacaccat
     6001 tgctcgtatg acaaaattga gcttgagcag cataatagct tggtttgtag tattctggaa
     6061 ggaaacgagc cattgtaaat gacataggat ttcccaacat aaaagcagat aagaacggta
     6121 acacaaggta acgagtaagc ggatttttcg ctgataattt ggctactttc gttactcggt
     6181 cttcacctaa aaaagcaatc agcgtattca ttgcaaccat cagcatcaag acaactggta
     6241 cgattcctgt catccagctt atgaatgttt ctgcacctgt ttggaacagc ctcataaatc
     6301 cctcagcaaa ttttgttatg atatccatcg tttgtcactc cttttttctt tgatcgtact
     6361 tttccatatc cgaaaattat tgagcatagc aataaaaggc aatgtaggtt gttcactttt
     6421 ttcatttcct gcctctattc gtaagtatac ttctctggca ttttctaaag ctgcttgtgt
     6481 caaacgatct tccgctcgaa ctgagggatg gtaacgatcg atgtaatgga tatcttcacc
     6541 cgcatagaca ggtaatgaat gaaattttgc caaagatgta accccttgca tttttctgac
     6601 ttctaaaact tgaccatgat cgtctatagc tagcataaca atcgtgcctg aacggatttt
     6661 cccaggtcgt ttaccaatcg ctacttttcc ttttcttcgc agttcgctat atgtttgatt
     6721 gaaatgcttt agttgcttcc aactgataac tgcttgcagc acataagctg aagctgcgaa
     6781 aactatgaat agatacatat cattcatgaa gtctctctcc tttacttttg attagtttta
     6841 aaaatgattc ctgtgtcggt gctaattcaa taaactcatc tattcccatt tttatgagtt
     6901 caaataggat atcgtatagc tcaaataatt cattttcaac ttcagccgtc atttcttcag
     6961 gtatggcaaa taaaaaaata actccatttc ctcctgtttc tgctaaacct tgatcagtta
     7021 ttagccctac tgataaaaca atgtgttgag atagctggtt ggttgtgtga ggaaaagcaa
     7081 attctttgtt aacaaatgtg ctttgctgag attctctttt caagatcctt ttttcaaatt
     7141 cttcatccac ataatagcct ctgatcaaag gagcgactaa tttgtttaaa tattcttgat
     7201 acgtcttttt ttctttccat atttgataga aaaaaacagt agaatgaaac ccgactaatc
     7261 gctttttttt gctttttgcc attgtgccct taaccaagag tcatcgaaaa gattagtcaa
     7321 atggatcacc ggtgtctctt catttacatt ttttaacgga atcgttgtaa aaacagcgaa
     7381 atatttgttt agatctactt tttcatattg ttcttctgaa acatgtgtta tttgtatatc
     7441 cgtccccaat atccttttga tttgtctttg gatcattaat gctgttcctt ttcctgtcgt
     7501 atgaacaatg gctacttttt tagaggaaaa atgctctttt gatatcgaaa tgctaactcc
     7561 aaatacaacg ttaggtagct gatctctact ttagagatct tatgttgcaa gatagcttct
     7621 aatgcttgta tgcattcgct agcgatttgg taagaaaaag gatacttttt ttctatctca
     7681 ccataaaata aatcagtcaa tcgataacga aaaattaccc gatttatcaa taaagagagg
     7741 tgctgtttca tctcttgata taagaaggac tcatccagat caatgagatc taatcgtcga
     7801 atacgcatca tcatggggtc gaaatataag cgcaaatcag agttagcttt tcttatcatc
     7861 gttatacttg tgttgatgat gttcaaagga aaactgacaa aatctttttc atattggctc
     7921 aaagttagtt catagatttc ttccaaccga ttgatcagtt cattgaattc ttggatatat
     7981 tcaaaacacc cactgtaacg attgatgggc ttcttcaaca gatagttact agagattcta
     8041 ttcaaagaaa taccgataac tttcgtcaaa agggtgattt cttgtttact agcattcgtc
     8101 ttcttctgaa aatgtttgat tgcttgtttt tcttctagaa cctgttctgt tttcgtctct
     8161 gaaaagtagg gagaaacaaa ataaaacaaa agtagtcgta attctagctc atctcctatt
     8221 attcttagtc ctttatttgg tgtgcctgta atctttacat caaaatcatt gatcatctct
     8281 tttaatgtct ttagatcctt gaccactgtt cctctgctga caccgacttc ttcagacaaa
     8341 tcatccatac gtaagaaatc ctctgtttcg atcagcctat ttagtaaata agcgattctt
     8401 ttggatgccg aattgaaatc actggctgtt ttcaattttc cttgcatgat ctcatcaaaa
     8461 aaatcaaaat cataaatttc aagttcaaaa tgtttgtttt tttggatgat ttttgcaata
     8521 ccggcaattt cttcattcaa ctccccaata tcttttttta tggtttgatt actggttttg
     8581 atttcttttt gcacttcctg gattgaaact ttcttgtgag tgactagtaa tttcaataat
     8641 tggtaccatc tgtttactag cgtcacgtgc gtccccccta tcctttatcc tcttgtttta
     8701 cctcctgaaa tattagtcgt gactccagtg atataacttg atctttctga aagataatag
     8761 cagactagat ccgccacttc ttgcagctta ccactccttc ctagagggat cgtacttgtt
     8821 cgactgtaac cgtttctcag ctgttcgacg cttatacctc tcgtataaga aagagcttct
     8881 tcatattctt gtgttctcaa accggtttct tccagtatcc ctggtgcaac acctaccaca
     8941 cgaacattcc tctttcctaa ctcttttgcc caagaacgag taaaactgtt catcgctgct
     9001 tttgtagcag cataacagct ctgtccttct gatccttcca atcctgattc agaagaaaga
     9061 ttcacgatca ctcctgatcc cttttgcacc aagattcttc caactgcttg gctcatcaaa
     9121 tatacacttt tttgattgac agcgatcatc ttatcaaaaa cttgttctga taattcatac
     9181 ttgctttttg gatgatttct atctattaga agagaaggaa tatttattcc tgcattattg
     9241 actagaccat cgatcgtctt gaaatgtttc attactttat atatcccttc ttctatgtcc
     9301 gttttagaag acacatcgac ttttaaagat agtaggtttt catgctgtat acggcattca
     9361 gttacatcaa aattcgctac ttgtgcattc tgcttcaaca aattttcgac gatacttctt
     9421 ccaattcctg aggaacctcc agttacaatt actacttttc ctgcaatatc caaccaatcc
     9481 ggcatatgtg atcctctcct tcttttttct tacaaatcca gtataaaagc tcactaaaaa
     9541 aaattaagac atgaaattta cagcttcagc taaaaaatgt gtactaatgg aagagcaaaa
     9601 aaaattttca tctttttgca tagattacta aaattcctga gcgttatata agcaatgttt
     9661 ctctttttga tatataattg ttttatcatt taaggaagga gaattctaat gaaaacttta
     9721 gatgctattg cattaacttt attgattgtg ggagggttga actggctgtt ggtaggcttg
     9781 ttcgaacttg atttagtagc tatgattgct ggtggttcaa caactatttt tgcgaagatc
     9841 atttatatcg tcgtaggtat ttgtgcgatc tactgcttga aatttttccc aatgattact
     9901 agaaaagttg atgaaagata ttagaaagct ataattatta aaaaagttat tatcttcatg
     9961 aaccttccct ttattgggaa ggttttttca ttttaaacca tctgcttttc tttgtttaag
    10021 tgatatactg aataaagata atctatttta ggaggagcta tcatgcataa agggatttac
    10081 gcaggcttgt tgggaactgt aatacttttt tctgcctgtg gatataaaac aaagaattct
    10141 gttaaagaat cttcagcagc catttcaaca actgaggttg gttcaactaa aagtacttct
    10201 actacttcca ttactaatac ttctactggt aatgtagatt cgcaacatag ccaatcaagt
    10261 attcagtcgt ttagcatttc ttcaacatct gaaatgcaag aaagcactgt accttctctt
    10321 ccattagaaa actctgacaa agcacttcta tttcttattg cccaccgttc cgaattacaa
    10381 agtgacgata ttgttactag cttttatcaa aaaattgatc gagattattt gttcacagct
    10441 tccagcaaac aaatccgttc ccaaggagga tctggaagcg ttggttttta tcgagtgtca
    10501 cccgaaggat cgattacgat gacagatgca aatggaacac cattttgatt agatgatatt
    10561 atacggattt acagtactag caagtcatta ttggtatcat tttttctgag aaagttatac
    10621 ggagtataaa taagcatttg cattaaccta ataattcgtt acagctattt ctcttatgaa
    10681 tatactcact tctctacttg ctctcttgat aaatttttgg tatctacttc attttcggtc
    10741 aactgagtta atgaaattta ttgtcgggcg gttttctaga ttcaattttt ctatatttgg
    10801 atagaagaat ttactttttt accttttgac atattttctt cttattattt taatgatccc
    10861 tttt
//
LOCUS       DANKHK010000081        10859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000081 DANKHK010000000
VERSION     DANKHK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(16..678)
                     /locus_tag="PW109_002013"
     CDS             complement(16..678)
                     /locus_tag="PW109_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511822.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(691..2193)
                     /locus_tag="PW109_002014"
     CDS             complement(691..2193)
                     /locus_tag="PW109_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511823.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2741..4420
                     /locus_tag="PW109_002015"
     CDS             2741..4420
                     /locus_tag="PW109_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2511824.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4547..5026
                     /locus_tag="PW109_002016"
     CDS             4547..5026
                     /locus_tag="PW109_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2511825.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5245..5925
                     /locus_tag="PW109_002017"
     CDS             5245..5925
                     /locus_tag="PW109_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2511826.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5933..7264
                     /locus_tag="PW109_002018"
     CDS             5933..7264
                     /locus_tag="PW109_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2511827.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7279..8241
                     /locus_tag="PW109_002019"
     CDS             7279..8241
                     /locus_tag="PW109_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2511828.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8352..8690
                     /locus_tag="PW109_002020"
     CDS             8352..8690
                     /locus_tag="PW109_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2511829.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8769..9476
                     /locus_tag="PW109_002021"
     CDS             8769..9476
                     /locus_tag="PW109_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2511830.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9579..>10859)
                     /locus_tag="PW109_002022"
     CDS             complement(9579..>10859)
                     /locus_tag="PW109_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511831.1"
                     /translation="QTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPK
                     KYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSD
                     TFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSS
                     EITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEI
                     GFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSS
                     FSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKE
                     QLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETF
                     YLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3451 a         1901 c         2290 g         3217 t
ORIGIN      
        1 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
       61 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
      121 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
      181 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
      241 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
      301 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
      361 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
      421 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
      481 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
      541 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
      601 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
      661 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
      721 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
      781 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
      841 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
      901 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
      961 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     1021 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     1081 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     1141 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     1201 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     1261 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     1321 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     1381 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     1441 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     1501 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     1561 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     1621 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     1681 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     1741 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     1801 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     1861 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     1921 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     1981 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     2041 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     2101 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     2161 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     2221 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     2281 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     2341 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     2401 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     2461 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     2521 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     2581 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     2641 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     2701 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     2761 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     2821 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     2881 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     2941 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     3001 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     3061 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     3121 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     3181 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     3241 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     3301 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     3361 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     3421 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     3481 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     3541 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     3601 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     3661 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     3721 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     3781 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     3841 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     3901 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     3961 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     4021 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     4081 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     4141 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     4201 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agtagcaata tgattgaaac
     4261 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     4321 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     4381 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     4441 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     4501 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     4561 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     4621 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     4681 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     4741 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     4801 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     4861 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     4921 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     4981 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     5041 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     5101 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     5161 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     5221 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     5281 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     5341 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     5401 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     5461 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     5521 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     5581 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     5641 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     5701 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     5761 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     5821 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     5881 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     5941 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     6001 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     6061 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     6121 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     6181 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     6241 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     6301 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     6361 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     6421 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
     6481 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
     6541 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
     6601 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
     6661 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
     6721 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
     6781 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
     6841 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
     6901 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
     6961 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
     7021 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
     7081 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
     7141 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
     7201 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
     7261 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
     7321 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
     7381 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
     7441 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
     7501 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
     7561 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
     7621 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
     7681 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
     7741 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
     7801 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
     7861 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
     7921 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
     7981 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
     8041 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
     8101 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
     8161 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
     8221 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
     8281 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
     8341 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
     8401 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
     8461 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
     8521 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
     8581 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
     8641 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
     8701 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
     8761 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
     8821 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
     8881 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
     8941 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
     9001 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
     9061 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
     9121 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
     9181 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
     9241 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
     9301 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
     9361 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
     9421 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
     9481 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
     9541 ccatttattt ggaataagag gatgtgacaa gaattttgtc acatcctctt tgttttgttc
     9601 tcataacctt ttttcatata cagttcatga accgctttta ttaacttgcc aaataacact
     9661 gtattgtgcg ttgtatccag atagaaagtc tccgtttcat ctgaaatttt ctcatgctga
     9721 tctgtaatga tcaaatcagc atgttctgaa tatgcggtaa ttttaacagc atctttgcga
     9781 aataactgct gcaaatggct ttgaagatga taatttcctg taaactgttt ggttatctgc
     9841 aaatggatga gtaactgctc tttcgtttct gtttgtagta agtgttggat caattgacta
     9901 aggtattgac tgatttggtt tctttcccca ttttttgaaa aaattgtatc gattttttct
     9961 cttaatttct tttcctgtat aaaagaacta gaatgattgg acgtggcttc tggaaagcaa
    10021 agtgtagaaa aactactgct ggattcaccc aacagttcga aaaaaacaac tgccaacaca
    10081 agaaaataaa aataaattgg tattttattt tctaaatggt tgagaggaaa ctgttgagtt
    10141 aagtaagtca tcaattgacc gacttgtcga caaataaggt ttttgcttgt ggaaaataaa
    10201 aaaccaatct cctgtttttg tcgaaaagag atttgatttg gaaaaaaaca atagaaaaaa
    10261 taattaaagt aggctctctc tgtttctgtc tcttcacaag ggcgactcac tgaaaaagaa
    10321 gtaaagtatt ctgaataatc atgtgtatgc tgcaataagt ccatcaattc agtgatttca
    10381 gaagaagttt ttggaaaata tgcctgttgt ttgtttcgta atatgaataa agttaacagc
    10441 aagtagatat ttttttcttg ttttgaaatt ttttctattt gctcatctgt actgttcatt
    10501 tttagtgcat ttaattcaag tgaggaaaat ggccaatcgt tacctccaaa tgtatctgaa
    10561 agaaaaaagt aaagatagag acggatagat aactcatttc cttcaagagt acagcaattt
    10621 tttacagata ggatccgtaa atggaatttt tttaaggaat gattgagctg ttttgtcagt
    10681 ttttcgagat aggagggcga tatataaagt tgttcaagta aatatttttt tggtacagca
    10741 gtttgtggat aattgattag taacatcaaa tagttaaaac gattggattc ttttaggtag
    10801 cataatttta acttagaaaa aacagctaac tttttagttt gatcggtttc tttggtttg
//
LOCUS       DANKHK010000082        10560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000082 DANKHK010000000
VERSION     DANKHK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(95..757)
                     /locus_tag="PW109_002023"
     CDS             complement(95..757)
                     /locus_tag="PW109_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2511832.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            941..2587
                     /locus_tag="PW109_002024"
     CDS             941..2587
                     /locus_tag="PW109_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2511833.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2578..3660
                     /gene="uxuA"
                     /locus_tag="PW109_002025"
     CDS             2578..3660
                     /gene="uxuA"
                     /locus_tag="PW109_002025"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2511834.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3768..5321)
                     /locus_tag="PW109_002026"
     CDS             complement(3768..5321)
                     /locus_tag="PW109_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511835.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIIKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            5469..6758
                     /locus_tag="PW109_002027"
     CDS             5469..6758
                     /locus_tag="PW109_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2511836.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(6884..7963)
                     /locus_tag="PW109_002028"
     CDS             complement(6884..7963)
                     /locus_tag="PW109_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2511837.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(8072..8512)
                     /locus_tag="PW109_002029"
     CDS             complement(8072..8512)
                     /locus_tag="PW109_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2511838.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(8569..8979)
                     /locus_tag="PW109_002030"
     CDS             complement(8569..8979)
                     /locus_tag="PW109_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2511839.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(9160..9594)
                     /locus_tag="PW109_002031"
     CDS             complement(9160..9594)
                     /locus_tag="PW109_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2511840.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(9694..10542)
                     /locus_tag="PW109_002032"
     CDS             complement(9694..10542)
                     /locus_tag="PW109_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2511841.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         3647 a         1907 c         1901 g         3105 t
ORIGIN      
        1 tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt
       61 taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac
      121 gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc
      181 gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga
      241 tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg
      301 tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt
      361 catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt
      421 cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac
      481 attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa
      541 caagattaat ttttgaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct
      601 gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca
      661 gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa
      721 tcgcttgata agcttgagat tgcaagctgc ttttcatctt tattcctcca aaatcatttc
      781 tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt
      841 aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac
      901 ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg
      961 gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc
     1021 atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct
     1081 ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac
     1141 tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt
     1201 tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt
     1261 agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat
     1321 gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg
     1381 ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa
     1441 tggtccaaag aagccatcac atacaatgag tattcttgct gcgctacttt ataaacgttt
     1501 tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag
     1561 tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca
     1621 aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat
     1681 tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt
     1741 ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac
     1801 agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga
     1861 aaaagctgga gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt
     1921 gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta
     1981 taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg
     2041 gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct
     2101 ggaagaagta attactgtgc gcttttctaa tccaaatatt cctgatatgc ctcagcgaat
     2161 cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat
     2221 ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg
     2281 gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc
     2341 actattgaca gactttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc
     2401 acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct
     2461 ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc
     2521 gaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg
     2581 gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga
     2641 cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta
     2701 tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta
     2761 aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag
     2821 cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc
     2881 atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta
     2941 gaagacggct catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa
     3001 atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat tgccgggatg ggaacctgag
     3061 cgcttagctc atatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt
     3121 cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc
     3181 aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattacc tcgaatcgta
     3241 aagaatcgag aagatttaga ttggttgtgc cgcatagtag acagccctta taacggaatc
     3301 actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca
     3361 gaattttgtc aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct
     3421 aatcgagatt tctatgaggc accgcataaa tccgaatacg gttcattaga catgtaccgc
     3481 atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga
     3541 atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct
     3601 gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag
     3661 tgaaaaaaac tgtaatttta ttatgtaaat aaggagggtg ttcaaattct tgatacataa
     3721 atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact
     3781 tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct
     3841 gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag
     3901 atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag
     3961 ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta
     4021 atgatcagat ctttactgat atagaaatca cgcgtatagt tcacaccaat cacaattcgc
     4081 ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt
     4141 tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat
     4201 tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta
     4261 ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg
     4321 aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa
     4381 aaggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt
     4441 ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa
     4501 atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca
     4561 gagggatcaa taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc
     4621 acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc
     4681 ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc
     4741 actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa
     4801 atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta
     4861 ttagaaaagg agatagacaa accgtacaat gctaaaaatg cattaatctt tttcattttc
     4921 gtatacaaat aagaggcact atagtttgtt tgctgactta aactgataat cgaatttgtc
     4981 tgacagctga acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca
     5041 aatagacgat aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt
     5101 ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct
     5161 ttgatgtata gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta
     5221 gtaataaaga aacgatgttc agataggaaa agaaaaatat ctaattttgt attgcttttt
     5281 ttcttcaaga gaaacgtttt aatcgcttct tgagtttgca tgaaagaggc tcctttttac
     5341 ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag
     5401 taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg
     5461 agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc
     5521 ttactataaa tcagacagtc cacacagagg agatagaaac agccaaatgg acagaaacca
     5581 agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag
     5641 tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcaggag
     5701 acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc gctttgtcga
     5761 ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg
     5821 ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac
     5881 taccgaacga tcatcatcat ttaacgccgt atagtcagcg gcagacaatt gagcaagatt
     5941 cagctatcga ctcaggagga gatcttgttg aagaaccaac agataaacca gaaaatgaaa
     6001 ataagccaga agtaccgcct acagaaaatc cagacggaga acagaaaccg gaaatagaac
     6061 ctggagaaga acccgataca gaaactcaac cagagccaga caatgaatca aaacctgaaa
     6121 taacccctgg agaaaaacca gatgtagacc cagaagaaaa accggatgtg actccagaac
     6181 cagatacgga ttctggaaac caaacagtac cggaaacaaa tccagacaca gataatgaaa
     6241 ctgaaaatcc agaaaaacca gaggtagccc cagaagaaaa accagatgta actccagaac
     6301 cagatacgga ttctggaaac caaacagtac cggaaacaaa tccagacaca gataatgaaa
     6361 ctgaaaatcc agaaaaacta gaggtagatc cagaagaaaa accagatgtg actccagaac
     6421 cagatacgga cgcaagagac caaggaatac cagaaaaaat aaacaaaaaa acaattcaag
     6481 aggatggaaa aaaagagagt aaaaaatcaa atctagcaat tctaaaaata aatgaagagc
     6541 agctgaataa aaaatcacga atatttgata gcgcccaatc agctgaaacc ttaaaaagta
     6601 gcaaggatac aacctttgct agtcctgaaa cgaaaaataa acagctacca aaatccggag
     6661 agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc
     6721 tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac
     6781 gcatttttca aataaaaaga aatatccaga aaacgacccc aaaataccag attgaaatct
     6841 gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt
     6901 cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga
     6961 tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca
     7021 caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat
     7081 aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc
     7141 ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg
     7201 ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata
     7261 cagccaaggc gattgtagtg agcaacgtgt ttaaaagagc gataacaaat tgagcttcta
     7321 ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc
     7381 aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca
     7441 taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct
     7501 gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct
     7561 caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa
     7621 aatcaataac ggactggatc atatcaaatg tttgatgaat aagcatcggg atatactttg
     7681 tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg
     7741 tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga
     7801 taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa
     7861 ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca
     7921 aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga
     7981 aaataagaaa gagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg
     8041 ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat
     8101 tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa
     8161 gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag
     8221 gagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat
     8281 ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat
     8341 ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact
     8401 gggtataggt aagattcagc ggcttgagga gggaagtgta gtgtctaatg atttccttag
     8461 aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac
     8521 ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac
     8581 tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg
     8641 cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat
     8701 ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac
     8761 agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca
     8821 tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc
     8881 aggagctgca atattcaatt cgaatgtacg atcgggatta tgaacttctc cagagcgtcc
     8941 gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat
     9001 attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat
     9061 aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca
     9121 aaaaaatatc agagacaaaa actaatctca tcataaacat tacatatatt tctcaaaaag
     9181 ttgttttctt tcttcaaaac ttacttcacg atggacctga tgaagcatcc ataaataacc
     9241 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     9301 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     9361 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     9421 gttttcatcc agcatatgga atctcgtgcc atacaaggta aaaatgactt cattattgcc
     9481 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     9541 tagtgctttc aaactatcat ttaatacaaa atctatttcc gttcctttat gcataaagat
     9601 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagt taaagaaaac
     9661 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     9721 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     9781 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     9841 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     9901 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     9961 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
    10021 agcataaaga aactcttcta ttccaaaaac aaacagatta agagaaacaa cgaatgcgtc
    10081 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
    10141 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
    10201 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
    10261 tattgcaatg attaaaggaa aaaacaagct tccgaaaagt actttaaaaa agggtttttt
    10321 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
    10381 tcggtcaaat cctaaaacag cttccagtaa tattcctaga ccgctgacac ctccagcagc
    10441 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
    10501 aatcttaatg tatgtccata taaattgtct cgtttctttc atttatccac ttccttaatt
//
LOCUS       DANKHK010000083        10218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000083 DANKHK010000000
VERSION     DANKHK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(552..752)
                     /locus_tag="PW109_002033"
     CDS             complement(552..752)
                     /locus_tag="PW109_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2511842.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1136..1285
                     /locus_tag="PW109_002034"
     CDS             1136..1285
                     /locus_tag="PW109_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511843.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIKQAVQE
                     LKKFA"
     gene            complement(1492..2259)
                     /locus_tag="PW109_002035"
     CDS             complement(1492..2259)
                     /locus_tag="PW109_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2511844.1"
                     /translation="MVNGLKVKIGPQFYLYEEGGISKLFIFDMDGLLLETGRLAYRAY
                     VKSAQKYDYEMRKEVYYLLTGQTEMAVREQMGILYGEDVPYIKWREAINQYKEKIVKE
                     DKRVYTKKGAEEILSFAKERGIHTIVASSNTREKIEMYLRMENLYDFFEQIISGDDVE
                     KGKPEPEIFLKACSKMNIPPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNG
                     KHKLKVDVSLYESNKADYEFYDLHQAKRFLESKRLYL"
     gene            complement(2268..3269)
                     /locus_tag="PW109_002036"
     CDS             complement(2268..3269)
                     /locus_tag="PW109_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2511845.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVNQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEHPDIICLCANLTE
                     ENYHMISAKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(3271..4312)
                     /gene="gnd"
                     /locus_tag="PW109_002037"
                     /pseudo
     CDS             complement(3271..4312)
                     /gene="gnd"
                     /locus_tag="PW109_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            4311..5159
                     /locus_tag="PW109_002038"
     CDS             4311..5159
                     /locus_tag="PW109_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2511846.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIIITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5805..6011
                     /locus_tag="PW109_002039"
     CDS             5805..6011
                     /locus_tag="PW109_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2511847.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6213..7214)
                     /locus_tag="PW109_002040"
     CDS             complement(6213..7214)
                     /locus_tag="PW109_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2511848.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVKKKKSPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGDSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     AFTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7219..9131)
                     /locus_tag="PW109_002041"
                     /pseudo
     CDS             complement(7219..9131)
                     /locus_tag="PW109_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            complement(9298..9804)
                     /locus_tag="PW109_002042"
     CDS             complement(9298..9804)
                     /locus_tag="PW109_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2511849.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDDQLVPQTKPWEILSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
BASE COUNT         2957 a         2031 c         1555 g         3675 t
ORIGIN      
        1 agaatgctaa atcactttac aataacggcg aaagcacaaa aaaacaaaaa aactattacg
       61 ttatttaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc atcacttgaa
      121 ggaatttaat gaactacttt ttcctctttg tcgtttttat atactcgtat tccttcaaac
      181 agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa aaatttagat
      241 gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga cagaccaatc
      301 gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat tttaaagaca
      361 tctcgtttca tattcaagat agtaatcttt ttatcaacat cactttattc gtattttcct
      421 ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc agcccattca
      481 acgaccaatg aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa
      541 gtcaattttt attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca
      601 acatcgtaag acactgcttg gccttcttct aaagttttga agccgtcacc ttgaatagct
      661 gagaaatgtg caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct
      721 gcgttgaacc attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat
      781 gtacgttttt tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata
      841 tatcgctcaa attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat
      901 aacacaagga taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa
      961 gaaaaagttg aacaaccgcg tttgattcat tttttgtttt cttgtttatt tgctttttga
     1021 ctttaaaatt tcttaactat gaaaaccttt caattggtgg tatacttata aatggaattt
     1081 cgttatcaac gcgttcattg cctttcagca agtatttaaa gaaaggagaa tggctatgac
     1141 attctataac aaagatatac aatatcgagt aaccttggat acagatttga atctatttgt
     1201 cgtttttgac aaagaaaata ccaatcatgt agcgacaggt gttacgatca aacaagcagt
     1261 gcaagaatta aaaaaatttg cataaggagt ggaaaccctc atatggacga ttctcgttct
     1321 tctctttaaa cgcatctact ggtagataat agtagatgcg ttttgtgttt gttctaaatt
     1381 gttcactaca aataaggtag atacttcaaa ataaaaaatt acttcttttt catctgttcc
     1441 ctcagtcgtg caggacaata aataaagagg actagaaaat agtcctcttt attagagata
     1501 caacctttta ctttctaaaa atcgttttgc ttgatgcagg tcataaaatt catagtctgc
     1561 tttattggat tcatataaac ttacatctac tttcaactta tgcttcccat tatgatcggg
     1621 caaccctatc aaatgatctt tgatgagaaa agacaaaatg cccgctcgcc ttgctgcttc
     1681 gatccctgca atcgagtctt caaatacaag tgcttcactt ggaggaatat tcatttttga
     1741 acaagctttc aaaaagattt ctggttccgg ttttcctttt tccacgtcat ctcccgaaat
     1801 gatttgttca aaaaaatcgt atagattttc cattcttaaa tacatttcta ttttctcgcg
     1861 tgtattagac gaggccacga ttgtatggat tcctctttct tttgcaaaag ataaaatttc
     1921 ttctgctcct tttttcgtat atactctctt atcttctttc acgatttttt ctttatattg
     1981 attgattgct tctcgccatt tgatatatgg aacatcttct ccgtacaaaa tgcccatttg
     2041 ttctcttacc gccatttctg tttggccagt tagtaaataa tagacttcct tacgcatttc
     2101 ataatcatac ttttgtgctg attttacata agcacgataa gctaaacgtc cagtttctaa
     2161 taaaaggcca tccatatcaa agatgaagag ctttgaaata ccaccttctt catataaata
     2221 aaattgaggg cctattttca cttttaaacc gttcaccatt ttcttcatca tccttttata
     2281 tagttgctct ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca
     2341 tctctgacac atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt
     2401 ggcgtaatca ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct
     2461 tctaaaacat ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct
     2521 tcttcatcaa ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga
     2581 tcgacttctt tagcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa
     2641 atgatatctg gatgttccag taattcttga agttctactt tttttgcacc aaagctttcg
     2701 atgtataaat ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc
     2761 aaaatttcga ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta
     2821 aacagcgtcc taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct
     2881 tgtgcttgtg tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca
     2941 gcatttcgtt caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct
     3001 tttaaatcaa tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcgtctttg
     3061 ttttcaaaaa aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct
     3121 ttaagtaact ccgctagttc ttttcctgga atctcttgat taacagtaaa gaaatgaacg
     3181 tctccaactg actttaatct ttccaagtgg tgtggaaaaa ctcttccgaa actgctggaa
     3241 ttaacgattg ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc
     3301 gccaaaccca tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga
     3361 aagattcctc atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc
     3421 tgtttcaacc gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa
     3481 attgggatgt tcgtttaact gatcttccac gatttccata agccagccgc gaatcaccga
     3541 accatgattc caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac
     3601 aagttggaat cctttaccga ttgcttgcat cattccatat tcgattccat tatggatcat
     3661 tttcaagaag tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac
     3721 atctttgaat aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca
     3781 ggcttgatgt cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc
     3841 attttttctg aaactcttct gctcgacgga ttgaatcttt ataataaaag tttccgcctt
     3901 caataataat gtctcctggt ccgagttttg gaaaaagttt tcttaacatg ttttcggtta
     3961 tctctccagc aggaagcatt aaccagatga tttttttctg ctcaaaagta gctagaaatt
     4021 cagagatctc ttgataagca ggaatgtcat tttctcttgc ctgactaaca gctgtactag
     4081 aaatgtcgag tccttgtact ttgtgatggt gctcttttaa gtttaaagct aaattcaatc
     4141 ccatttttcc caaaccaatt acaccaattt ccatgataac gcctccattt cttataccct
     4201 cattatacaa aaaaatattt tttgtttgat tttttcctac tctccttgta tttttaacat
     4261 taacgagaac ggaaaaaatt ataatcttat tatgaaaagg aggaattctc atgagcgtct
     4321 ccataagact acaaatcaaa acaatgtaca acgacttcag cccaaaagaa caggctatcg
     4381 ccgattatat tttagaaaat ccaagcaaag taagtcatag tccgattagc aatttagcaa
     4441 gtgaactagg tattgctgac tctacttttt ttcaatttac aaaaaaactt ggttataatg
     4501 gatttaaaga ttttaaaatg gccatgttga tccaagaaaa tgatctatct gctatttcta
     4561 tccacgaaaa tattcagaaa acagatactg agctcactat ggcgcaaaag gtgttcgagt
     4621 ctagtatttc aactttaaat gacacaaaaa agttgttaca acaaaaggat ctaaaaaaag
     4681 cggctactat tatcaatgaa tctaaacgtg tctatttttt cggtgttggc ggttcagaga
     4741 ttgtagcaac tgacgcttat cataaatttt tgcgttcccc tattgcaact tctcacagca
     4801 cggattacca tattcaattg atggaagctt ctttattgac ggaaaaagac tgtgctgttt
     4861 tgatttctca cacagggcaa tcaaaagaaa caatccatat tgctgaaacc gtaagaaaaa
     4921 ctggagcaaa aacaatcatt attactagcc aagctaattc ttcccttgct aaacttggcg
     4981 atgtcgtttt tatttctatt tctgaagaaa cagaattccg atctgaagct ttagcttccc
     5041 gtatttctca actaagcatt ttggactctc tatatgtgat tttgatgttt tacaacgaaa
     5101 aagaatcacg tcgttctatc gctaaagttc gacatacgat ttctgaaatc aaagaatagc
     5161 accataacat aaaaagcgaa aaatccataa aacaaaaaaa gccactacca aatatttggt
     5221 agtggctttt ttggttctgc aagggtggta cagaacatca atagtcggtg ggttagaacg
     5281 tatgtcatcg catctttgga gaaggttgta ttggtattat tcgagttact tgttagatgc
     5341 gataacaaag ttagtataca gtagtaaaaa atttatcttt tctttttttt attatttttg
     5401 tttcttattt tctaaaaaat taaaaaaaat tttggtaaat taagtatttc cttgataaca
     5461 ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt tgatatgatt
     5521 gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc ttttgggggt
     5581 atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga cgtatgatgg
     5641 taaactttgt aactatctga ttgccgaatc attaattgct tatcattagc catctttgtt
     5701 tcaagttcgc tggatgatca ttttattcac atcctaacaa acttgtcaca tttatagatt
     5761 cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa gctatccata
     5821 tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt tgttcccagc
     5881 cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta ccactcggct
     5941 ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac
     6001 aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg tgtgatattc
     6061 ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac aagttacaat
     6121 attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg gagctaagtg
     6181 acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat tactaacttt
     6241 tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg gttcaaaaca
     6301 gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc aaatacagaa
     6361 gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa gaatgtgttt
     6421 tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc tgattgttca
     6481 aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct agagatttta
     6541 tcacttactt gagtaaatgc agaaggatcc aacagaccag acaaagaaat aaacttcgta
     6601 atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc ttcattttga
     6661 agtgctaaca aatattctcc actaatttgt gcacctgccg aatctccacc aatcgaaata
     6721 ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc ttcgttcaat
     6781 tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat atttacgatg
     6841 atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt tttatcccct
     6901 cctacatatg cgcctccatg catccagaaa agaatcggtg actttttctt tttcactttc
     6961 ggtgtgtaga tatccatatt attatttttg tattttgaag gataggatat ttcttttttt
     7021 atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac ttgttctttt
     7081 tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt gatttgatta
     7141 atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat cactattcgc
     7201 ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga taatcgtgct
     7261 aatagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc cagttttcgg
     7321 caacattctt tttttgcatc tgtctttttt tgtactttca ctgtcatctg aatcggttgc
     7381 gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc tgcattctct
     7441 gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatggta aataatagtt
     7501 ggtatcgtct cattaaaaac cccttcagtc ggtccttcaa catttgctaa ttcgtatcct
     7561 tcgaatgtct tttgctctgt catataattc tctccataag ctccgtaaag ctggatttcg
     7621 tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt tatctgatca
     7681 tgaaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc
     7741 tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc tcctgataac
     7801 tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc atggataatc
     7861 aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt atccaaatgc
     7921 atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatattc atgctcttga
     7981 tccccaatga tgtctaactg attttttatg gcatccggta gtgcggggac tactgtctgg
     8041 ttgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc aataattcct
     8101 ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc tcccatcgaa
     8161 ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt tatccagtaa
     8221 aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct attaccagtc
     8281 gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt gtattcttct
     8341 gtaatgatcc cgttattcca attccctcga aatccgccat ggatcatgac tgttttatct
     8401 gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg tttgtttagt
     8461 acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt aggcgttgtc
     8521 ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa agcgatatcg
     8581 tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc ataatcatta
     8641 acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc tgttggtcca
     8701 ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc attgaaattt
     8761 tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac tttagcagaa
     8821 gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg gttatctgtg
     8881 gtagcggtcg tttcttgatc tgtgctagta acagatgtct cagccgttgt ttcagtattt
     8941 gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt ttctgtggtt
     9001 tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt ccattgcggg
     9061 acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg ttttgttctc
     9121 aagctattca ctccttttta tgtttctagg agaattgtat gtaaacgcaa acatttttat
     9181 acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc attttacgta
     9241 ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag aaacaactta
     9301 ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc
     9361 ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca tcataagctg
     9421 gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg cttgtgcccc
     9481 acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat tagtcttata
     9541 aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag ataattccga
     9601 caatatctcc catggttttg tttgtggcac taattgatca tcttcgtgtt gaacgaaaat
     9661 aactggcttt ccaatcgttt cgtatgtttt gattctttta ttcactaatg ctgtcactgt
     9721 ttctaattcg tgtatcggtg aagaatcaaa acatacccct ttttgtaaat cgatcactac
     9781 tattgcgtcg gctgtgtttg acatacgtat gccttctttc ttttttctag ttctttcttc
     9841 taatgatgtt tcgtttacta ctattttcca ttttatggtt agaaatcaaa aaaatatatt
     9901 ccattttctg atttttttat tggggttcct ttgatagcat ttcttatctc gttcgttcct
     9961 ttatacgtga caacattttc ttattttaca cctataaatg gcacttttcg actatttctt
    10021 catgttatta tttcataaat atttagctat tggaggaatg tatcatgatt aaattatata
    10081 caggttctgg ttgctcgtct tctagacgcg caaaacgttg gcatgtttaa agaaaaacca
    10141 tttataaaag atatcttgaa aagatagaga aaacaaaaaa caaccacttt cagttgacta
    10201 ctgagcagtt gttatctt
//
LOCUS       DANKHK010000084        10103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000084 DANKHK010000000
VERSION     DANKHK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            24..1316
                     /locus_tag="PW109_002043"
     CDS             24..1316
                     /locus_tag="PW109_002043"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2511850.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2103..2966
                     /locus_tag="PW109_002044"
     CDS             2103..2966
                     /locus_tag="PW109_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2511851.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3095..4717)
                     /locus_tag="PW109_002045"
     CDS             complement(3095..4717)
                     /locus_tag="PW109_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511852.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            4966..5487
                     /locus_tag="PW109_002046"
     CDS             4966..5487
                     /locus_tag="PW109_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2511853.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5673..7403)
                     /gene="spxB"
                     /locus_tag="PW109_002047"
     CDS             complement(5673..7403)
                     /gene="spxB"
                     /locus_tag="PW109_002047"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2511854.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGN
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7568..8386
                     /locus_tag="PW109_002048"
     CDS             7568..8386
                     /locus_tag="PW109_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2511855.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8489..8686)
                     /locus_tag="PW109_002049"
     CDS             complement(8489..8686)
                     /locus_tag="PW109_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511856.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            8855..9433
                     /locus_tag="PW109_002050"
     CDS             8855..9433
                     /locus_tag="PW109_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2511857.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            9528..9665
                     /locus_tag="PW109_002051"
     CDS             9528..9665
                     /locus_tag="PW109_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511858.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         3083 a         1811 c         2019 g         3190 t
ORIGIN      
        1 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
       61 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
      121 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
      181 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
      241 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
      301 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
      361 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
      421 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
      481 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
      541 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
      601 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
      661 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
      721 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
      781 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
      841 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
      901 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
      961 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
     1021 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
     1081 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
     1141 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
     1201 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
     1261 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
     1321 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
     1381 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
     1441 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
     1501 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
     1561 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
     1621 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
     1681 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
     1741 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     1801 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
     1861 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
     1921 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     1981 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
     2041 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
     2101 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
     2161 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
     2221 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
     2281 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
     2341 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
     2401 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
     2461 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
     2521 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
     2581 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
     2641 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
     2701 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
     2761 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
     2821 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
     2881 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
     2941 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
     3001 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
     3061 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
     3121 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
     3181 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
     3241 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
     3301 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
     3361 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
     3421 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
     3481 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
     3541 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
     3601 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
     3661 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
     3721 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
     3781 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
     3841 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
     3901 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
     3961 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
     4021 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
     4081 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
     4141 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
     4201 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
     4261 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
     4321 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
     4381 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
     4441 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
     4501 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
     4561 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
     4621 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
     4681 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
     4741 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
     4801 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
     4861 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
     4921 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
     4981 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
     5041 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
     5101 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
     5161 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
     5221 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
     5281 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
     5341 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
     5401 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
     5461 acacatatcg tctatgcttt tagataagta aaatcaaaca aataaaaaag ccggcttccg
     5521 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
     5581 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
     5641 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
     5701 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
     5761 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
     5821 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
     5881 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
     5941 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
     6001 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
     6061 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
     6121 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
     6181 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
     6241 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
     6301 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
     6361 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
     6421 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
     6481 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
     6541 gttggattaa aaaatgcacg tccaaatggg aagtcgttac cgacaaataa gatcagatcg
     6601 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
     6661 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
     6721 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
     6781 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
     6841 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
     6901 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
     6961 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
     7021 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
     7081 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
     7141 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
     7201 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
     7261 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
     7321 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
     7381 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
     7441 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
     7501 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
     7561 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
     7621 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
     7681 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
     7741 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
     7801 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
     7861 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
     7921 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
     7981 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
     8041 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
     8101 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
     8161 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
     8221 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
     8281 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
     8341 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
     8401 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
     8461 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
     8521 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
     8581 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
     8641 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
     8701 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
     8761 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
     8821 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
     8881 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
     8941 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
     9001 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
     9061 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
     9121 gtttgcaact tcatggatca gtgagtacat caatagcttg gctccagaaa tgacttatgg
     9181 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
     9241 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
     9301 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
     9361 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
     9421 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
     9481 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
     9541 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
     9601 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
     9661 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
     9721 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
     9781 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
     9841 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
     9901 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
     9961 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    10021 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    10081 aaagtaggat caggcagaat ata
//
LOCUS       DANKHK010000085        10050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000085 DANKHK010000000
VERSION     DANKHK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(90..1025)
                     /gene="murB"
                     /locus_tag="PW109_002052"
     CDS             complement(90..1025)
                     /gene="murB"
                     /locus_tag="PW109_002052"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2511859.1"
                     /translation="MNKEEIVESLATITLLFDEPLMNYTFTKTGGPADVLAFPKKQDE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1098..2018)
                     /locus_tag="PW109_002053"
     CDS             complement(1098..2018)
                     /locus_tag="PW109_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2511860.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKCIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETEVLKQKEVVRSHELCEKVLKDHYRHQL"
     gene            2131..2883
                     /gene="xth"
                     /locus_tag="PW109_002054"
     CDS             2131..2883
                     /gene="xth"
                     /locus_tag="PW109_002054"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2511861.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQTIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPRLADAKIHTDIMGSDHCPVELDLK"
     gene            3623..4156
                     /locus_tag="PW109_002055"
     CDS             3623..4156
                     /locus_tag="PW109_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2511862.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4645..5169)
                     /locus_tag="PW109_002056"
     CDS             complement(4645..5169)
                     /locus_tag="PW109_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2511863.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYERNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5162..5635)
                     /gene="tsaE"
                     /locus_tag="PW109_002057"
     CDS             complement(5162..5635)
                     /gene="tsaE"
                     /locus_tag="PW109_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2511864.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASEADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5758..6738)
                     /gene="pta"
                     /locus_tag="PW109_002058"
     CDS             complement(5758..6738)
                     /gene="pta"
                     /locus_tag="PW109_002058"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2511865.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6891..7571)
                     /locus_tag="PW109_002059"
     CDS             complement(6891..7571)
                     /locus_tag="PW109_002059"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2511866.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7697..8566
                     /locus_tag="PW109_002060"
     CDS             7697..8566
                     /locus_tag="PW109_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2511867.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDTGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8592..9107
                     /locus_tag="PW109_002061"
     CDS             8592..9107
                     /locus_tag="PW109_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511868.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSK
                     EKPLSYRFDVN"
     gene            complement(9327..9672)
                     /locus_tag="PW109_002062"
                     /pseudo
     CDS             complement(9327..9672)
                     /locus_tag="PW109_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2962 a         2036 c         1798 g         3254 t
ORIGIN      
        1 aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc aggacggcca ccgtcctgct
       61 cttttactta ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat
      121 attgcttgtt tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct
      181 ttgattactt cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg
      241 atattgacga tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct
      301 tgcaatcctg cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca
      361 cttccgcatg aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc
      421 attcttgctt tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg
      481 atagcacgta attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc
      541 aaagttttca atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc
      601 tctccgccat acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg
      661 caagcaaatt caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt
      721 gaggcacctg cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc
      781 agcatgatga ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct
      841 aataccatcc aaggtatatc atggattcgg caataatcga tgatttgctt tacttcgtct
      901 tgctttttcg ggaatgcaag gacatcagct ggtccgcctg ttttcgtaaa cgtatagttc
      961 atcaatggtt catcaaacaa taaagtaatc gtggctaagc tttcaactat ttcttcttta
     1021 ttcactgagg attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac
     1081 aatcttgcca gtcttgttca aagttgatga cggtagtgat ctttaagcac tttctcacat
     1141 aattcatgac ttcttacaac ttctttttgt ttaagcactt ctgtttccga tttacctaaa
     1201 cggatctgag aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt
     1261 gtccagtcgc caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc
     1321 agatcgttta cttcataagt accattttta cttgttactt gatatttttc tatattagcg
     1381 ccacttgcta aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg
     1441 gcatattcca aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca
     1501 tctaataaat acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaattct
     1561 gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctggtata
     1621 catttcaatt cttctaccat aggagcaaag cgtcggttga aaccgatcat caaaattatt
     1681 tggttcttat cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt
     1741 ttgtcaatat aaacatggat accattttct agacattttt tggctaattc ataatgtact
     1801 tttgttgctg cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt
     1861 tcatatagat gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt
     1921 gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt
     1981 tctgcgatat tccctacacc gattacacca atcttcatct actttcctcc tggatcattt
     2041 agttactttc atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta
     2101 aggaaattaa ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg
     2161 cttcgtgcca tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt
     2221 ttctgtctgc aggaaacaaa actccaagca ggacaaattg atttagatct tccaggttat
     2281 tatcaatatt ggaattacgc agaacgaaaa ggctattccg gcacagcgat ttttgctaaa
     2341 aaaccagcat tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt
     2401 ctgattactt tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa
     2461 agcgaattaa aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg
     2521 gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgcgcaccaa
     2581 acgatcgatt tgaaaaactg gaaaacgaat caaaaaaatg ctggatttac tccagaagaa
     2641 agagcggctc tttctcgact gttggataac gggtttatcg atacattccg ttatttttat
     2701 ccaacccagg aaggtgtcta ctcttggtgg aactaccgat tcaattcaag aaaaaacaat
     2761 gctggttggc ggatcgatta ctttttgacg agcaaagatt tagagccaag actagctgat
     2821 gcaaagatcc atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa
     2881 taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg
     2941 ctttccacat tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
     3001 atcgagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     3061 gctcttcata tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacacct
     3121 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagaaac aaatggaaaa
     3181 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
     3241 gtttagtgga gaggttgtaa aatcagcgtt ctgacctgag tattagagaa acaaatggaa
     3301 aaatagttcc tcatattttg ccgttttttg agctaatacc gaggatcagc tgattgaaca
     3361 ccgtttagtg gagaggttgt gaaaaagtcg tttagtctcg agtattaaaa aaaactttgt
     3421 gaaaatggct ttccacattt ttgcataaat ttcgcttaat accgagaagt catgatccct
     3481 atctaactcc aaatcacaag gaataagttc aaaaactagc ttttttcaac aaatattaca
     3541 taaaaaattc tatgtttttt gttaaattta tttgcttttt caaaagtatg ttacagtaat
     3601 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3661 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaacagcc agatcgttga
     3721 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3781 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3841 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3901 ggtcttagcg ggatgcttag attactatca tcttgaacag cctaattatc tttctttatg
     3961 tacagtgaaa agtagccgtc gcttgtttcc tgaatttcca aatcatcgat tgaacactgt
     4021 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4081 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4141 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4201 tcactccgag tcacaaggag caatttcaaa aaatagctcc tcatgtttat taaatattgt
     4261 gactggtgac cgaggagctg gcaggagaac accgtataag gaagaggttg tgaaaaaact
     4321 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctgatatt tttcatttgg
     4381 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttgtgaaatc
     4441 agcgttttga tctgagtatt agagaaacaa acggcaaaat agttcctcat attttgctgt
     4501 tttttgagct aataccgaag gttagctgat tgaacaccgt ttattcagaa aaagggactg
     4561 tgacacgtat tttgtcatag tcccttcgtg tgtttttctg ccttctcaaa aggacgacag
     4621 tttctcactc ttttcctatt tcttctattc aatcaaataa ctcattttat atacatctaa
     4681 aaattcacct agatctgttc ttgcccctct aggcatgacc gcttcgattt ggaagcccat
     4741 ttttcggtaa aggtgtacgg ctctttcatt acgaacttga acgtcaagct ccaatcgaaa
     4801 aagtacaccc atttccttcg cccaactgat gatttcttct agcataagag tgcctagtcc
     4861 cattccccaa tattcctgca gaatactgat accgacttct cccacgtgag acaaacgaaa
     4921 ttgactgtct gccttgacag atgctgttcc aataatcgtc ccttcataga ttgcaagcag
     4981 caataaatta ttattccttt cgtatatata atccaactct tcttctaagg tttctggtgg
     5041 taacaggagt tctgcttcat ccaaaactaa aaattctgtc tccttattga cttgtcccat
     5101 catcaaaaga atgtcttttg catctcctgg cactgcttcc cgcaagatga tcccaacttc
     5161 ttcactcatg attttcagcc catttttcta gaattcgctg tttgaaggtt tcactttgct
     5221 ccccgtatga gattagtttc acataccgct tttctaaatc tgtatcactt ttttctaaaa
     5281 tcagttccaa ataatcctcc ggcagtgctt cttctaatag atttccccat tcgacaacgg
     5341 aaagcccatc accttcaaaa tattcatcta accctaaatc tgcttcgcta gctgctaccc
     5401 gataaatatc catatgatac aacggaatcc gaccttgact atattctcta ataatcgtgt
     5461 atgtcggact tttgatcatt tgcgttatcc ctaatccttg ggcaattcct ttcgtcaacg
     5521 ttgttttacc cgcacctaga tcaccagtca atatcagatt gtcacctggc attgcggaat
     5581 gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc tgatagttcg atcatttttc
     5641 atcttctccc gctcttcttt ctttttgcca gtatatcgcg taatgaagtt tttttaaaga
     5701 aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc tagttcgtgt gtatttgtta
     5761 atcaatcaat gtttgtgctg ctgtgataat cgataattta tatacatctt cttcatttgc
     5821 tccacgagac aaatcagaaa ccggtttgtt caagccttgt agaattggtc cgattgcttc
     5881 aaagttaccg aaacgctgag caattttata gccgatattt cctgactgca attctggaaa
     5941 tacaaagact gttgcttgac cagctacttc tgaatctggc gctttcagtt gtgcaactga
     6001 aggaacataa gaagcatcaa attgcaattc gccgtcgatc atgtattgtg gcgctagttt
     6061 tttagcgatt ttagttgctt caactacttt gtctacttct ggcgatttcg ctgagccttt
     6121 tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg aacaattctg ctgtttttgc
     6181 agaatctacg gcgatttcag ccaattcttg cgcagttgga ttcacgttga tcgcacaatc
     6241 cgaaaagaca tatttttctt gatctcttcc acgtaccatg atcatcgctc cgcttgtccg
     6301 actgatgcca ggctttgttt tgatgatctg aagtgctggg cgaacagtgt cgccagtcga
     6361 atggatcgct ccagagacca ttccgtctgc aatccccata tacgtaagca tcgtaccaaa
     6421 gtaattcaca tcttttaaaa tcttttctgc ttgttctttg gatgcctttc catttctgcg
     6481 ttcaacaaaa gtatccacca tttcatccca cttatcgtaa ttatcagggc tgatgatcgt
     6541 aaagctagat gttttgattc ctctggcatg cgctgcattt cttatttctt cttcgttccc
     6601 tactaaaatg ggttccacaa gttcttctgc tttcaaccgg gcagctgcgc ctaaaatacg
     6661 agcatcagta gcttccggga aaacgatttt gatcccacgg cgaacaatct taaatcttaa
     6721 actatcaaac aattccacaa gatgacctcc agacgttttt atatgtatta tcatacacta
     6781 ttccagaaaa aaagaacagt ttattttatc tgtatcagtc aaaaatgctg gaaaaattaa
     6841 gtttttcaca tacaaagcga attgcactat aacaggaaag tgctgtttta ttacactgtt
     6901 tcaggcagtt gccaatcaat cggttcttct cctaacgcaa ggagcgcatc attcgttttc
     6961 gagaagggtt tggaaccaaa aaagccacga cttgcagata acggactggg atgtggagat
     7021 gttatgatga tatggcgact cttatcgatc attttgattt tttcttgtgc cggttttccc
     7081 cacaagataa acacgattgg tttttcccgt tcatttaatt tttcaatgat cgtgtctgtt
     7141 aatctttccc aacctttccc gcgatgagaa tacgcttgcc cttcccgaac ggtaagaacg
     7201 gtgttgagta aaaggactcc ctgtttagcc catgacacta gatttccatg ttgaacaggt
     7261 gcgattccaa gatcatcata aagctcttta tagatatttc ttaacgatgg tgggatcttt
     7321 acgccaggtt gcacagaaaa agataagcca tgagcttgat tttctccatg ataaggatct
     7381 tgtcctaaaa tcaccacttt aacttcttca taaggagtca attccaatgc ttcatagata
     7441 tgatacatat ctggatggat cttttgggac gcatattctt tttttaaaaa ttctcttaga
     7501 tgcagataat aatcttttga aaattctgag gacaaaactt cttgccaact gttatgaatg
     7561 atttctttca aaaactccac ctcctaataa aaagaataac tcacattttc ttcaatcaca
     7621 agggacaatc ttcaccctca gcacttgatt aatatggtaa actaggaaca agaagcagaa
     7681 aggaacgata atcattatga tcaaattaat tgcatcagat atggacggca cattattaga
     7741 tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata cgttttgcaa atgataccgg
     7801 tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa gctcgcgcag ctttagatga
     7861 ggtaggaatc gattgtgcga tgatcacttt gaacggggca caggtatttg ataaagaagg
     7921 aaattcattg tttaccgtgt cgattccaaa taatcaagca atctctgttt tagatattct
     7981 agatgctaac ggtatttact atgaagtagc aaccaacgat ggtctttatt ctgaaaatca
     8041 agcgaaacga attgaaagtt ttgcttccat ggtggctacc catttacctc accttacgta
     8101 taaaatggcg attgccatgg cctcagctaa gctggagtta ttgcacatca catacgtgga
     8161 cagtattcgt gctattttgg aagaaaagaa tctggaagtt ttaaaaatta tttgttttca
     8221 tacagaaggg ccagctgtat taggtccagt tggaaagcag atttctcttt taggcgatct
     8281 agccgttact tcctctggtc aaaacaatat cgaagtgaac cataaaaacg ctcaaaaagg
     8341 aattgctgtt gcccatgtcg ctcacgagcg tgggatttct ttagaagaag tcatgacgat
     8401 tggcgataac ttcaatgatg tcagcatgct gcaagtagca ggagtcagtt tcgcaatggg
     8461 aaatgcagaa atcgaagtaa aagattatgc caaatataca acggacacca accttgaatc
     8521 tggcgtagga aaagccatat tgcgcgcgat tgcagaagac ttgtagtttt atctagaaaa
     8581 gagggatata gatgtcagaa tttttcattc aggaaaagca gcttggacgt gcaactcgga
     8641 caattgtcaa aaacgaaaaa ggacattccc tgtttttgat ggttggtcgc tggggaacaa
     8701 agggcgatgc attgtctctt tatgccatga atggcgatct cgttgctcac atcaagcaga
     8761 taagcctgac attcggtacg cgttttgagc tgtataaaga gttcgaaaag gtgggaacca
     8821 tgcagaaaat attcaattgg cccggtgact tttattatat tcgtagactt cactggacag
     8881 tccaaggtga tatctataat catcaatatc aaatccatca tttcaatcag caaatcatga
     8941 aaatggataa agcaacactt ttttcaggag attactatgt attggatgtc tcaaatgatg
     9001 aagatgctcc agtttgtatt tgtattgctg cggttttaga ttactggctc taccgtaaag
     9061 acaaaagcaa agaaaagcct ttatcctatc gatttgatgt gaactagaaa aaatgaaagc
     9121 aagctaacaa taaacaatct ccaactctaa gtcaaaatta agaatctacg atattgggag
     9181 tcgttacttg atcgagtaac gactcccaat atttcttatc aaaaatagga aatatcaaaa
     9241 ttcccttcga gacggaggta ttcaaacact cttgatgatg aactaaaaaa gtgctgtttt
     9301 atccttctat attctcataa cacatattaa gactttaatc gtccgatagg acatcctcca
     9361 ttcgatctga ctgttcctct atataatccc tacataccaa aatcttcctg tagcaaaatc
     9421 tttgtcgtaa gaccaagttt gtaaatataa ttgcccacaa taaataccat cattatatat
     9481 aattgtattt gttagaccgc ttaaagtagc cgatcgtctt gtgtggtaga taactttact
     9541 acctctgcta ttgatagtac tcattacttg ctcagcattt aaatcatttg tatcagcatg
     9601 gattttttgt gaaaaagcgg ttaatcccca tatagccatt attaaaataa ataagttctt
     9661 aaattttttc acttatatta tctccttcct gatatttatt tttgaaggaa tcggtaaagg
     9721 taaggaatgg tgataaaaat atccctcata ataacctcga gttccatttg aaaggggcat
     9781 ttccctatat tttccaagcc aacctcgata ggctccagat tagaagaatg caacagctgg
     9841 ggcaggattt ccagtcgttg tccatccgct tgtccaataa cgagtcaata tagcttgtgg
     9901 ggaaatagtg ataaacttca tttgatcaaa atcaaaaata gtagtttcta caatgtgaga
     9961 aggagcatcc tcaccatagc taaaatcttt ttcttcagca tggacacagt tattacttaa
    10021 taaaacagca aaaaacagct agataaaaaa
//
LOCUS       DANKHK010000086         9994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000086 DANKHK010000000
VERSION     DANKHK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(76..621)
                     /locus_tag="PW109_002063"
     CDS             complement(76..621)
                     /locus_tag="PW109_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2511869.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(633..1325)
                     /locus_tag="PW109_002064"
     CDS             complement(633..1325)
                     /locus_tag="PW109_002064"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2511870.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1344..1628)
                     /locus_tag="PW109_002065"
     CDS             complement(1344..1628)
                     /locus_tag="PW109_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511871.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1641..2204)
                     /locus_tag="PW109_002066"
     CDS             complement(1641..2204)
                     /locus_tag="PW109_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2511872.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2432..3094
                     /locus_tag="PW109_002067"
     CDS             2432..3094
                     /locus_tag="PW109_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2511873.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELGTHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3218..4414
                     /locus_tag="PW109_002068"
     CDS             3218..4414
                     /locus_tag="PW109_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2511874.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4515..5891)
                     /locus_tag="PW109_002069"
     CDS             complement(4515..5891)
                     /locus_tag="PW109_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2511875.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5928..6794)
                     /locus_tag="PW109_002070"
     CDS             complement(5928..6794)
                     /locus_tag="PW109_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2511876.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6883..7908)
                     /locus_tag="PW109_002071"
     CDS             complement(6883..7908)
                     /locus_tag="PW109_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2511877.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLVFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7926..8600)
                     /locus_tag="PW109_002072"
     CDS             complement(7926..8600)
                     /locus_tag="PW109_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511878.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8784..9677)
                     /locus_tag="PW109_002073"
     CDS             complement(8784..9677)
                     /locus_tag="PW109_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2511879.1"
                     /translation="MKKKTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQLRKNNR"
     gene            complement(9751..9876)
                     /locus_tag="PW109_002074"
     CDS             complement(9751..9876)
                     /locus_tag="PW109_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2511880.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3029 a         2163 c         1739 g         3063 t
ORIGIN      
        1 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
       61 aattactttg tttttttata aaatttctgc gcctagtgtg gattcaaaat gtctcaatgc
      121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
      181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
      241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
      301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
      361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
      421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
      481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
      541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
      601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
      661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
      721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
      781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
      841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
      901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
      961 cttgcttcat aatatagagg cattcctggt aattgtcctg gtttgtagcc aaatcctgtt
     1021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
     1081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
     1141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
     1201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
     1261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
     1321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
     1381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
     1441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
     1501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
     1561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
     1621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
     1681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
     1741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
     1801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
     1861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
     1921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
     1981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
     2041 tcaaaagcaa tgatcccaac accgccagga tgaaatacca gttctctctt cgaagttcct
     2101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
     2161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
     2221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
     2281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
     2341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
     2401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
     2461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
     2521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
     2581 aaaacaagaa gaaattcctg cactgactga cgaacttgga actcactatg aaaaaagcgg
     2641 tatgacagcg aatgaaattt cattttttcg gcaaacaatg aatcaaacaa aaaatgagat
     2701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
     2761 tgatccagtc aaagcagcaa aagctttatt caaagaattg gtgaaagaac caaaccgact
     2821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
     2881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
     2941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
     3001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
     3061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
     3121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
     3181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
     3241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
     3301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
     3361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
     3421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
     3481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
     3541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
     3601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
     3661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
     3721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
     3781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
     3841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
     3901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
     3961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
     4021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
     4081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
     4141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
     4201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
     4261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
     4321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
     4381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
     4441 aacgcccaac aacagtttat tacaactgtt gttgggcgtt tttcttatct ttcttctaac
     4501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
     4561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
     4621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
     4681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
     4741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
     4801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
     4861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
     4921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
     4981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
     5041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
     5101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
     5161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
     5221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
     5281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
     5341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
     5401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
     5461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
     5521 tagccagacc atccggaagc aacagaggaa actgctagta aaaattcccc gatcaacaac
     5581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
     5641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
     5701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
     5761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
     5821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
     5881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
     5941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
     6001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
     6061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
     6121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
     6181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
     6241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
     6301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
     6361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
     6421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
     6481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg taaccctaca
     6541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
     6601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
     6661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
     6721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
     6781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
     6841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
     6901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
     6961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
     7021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
     7081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgtgc
     7141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
     7201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
     7261 accaacaatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
     7321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg aaaaactgtc
     7381 tagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
     7441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
     7501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
     7561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
     7621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
     7681 ggttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
     7741 cagataaaaa accagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
     7801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
     7861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct tagccgctca
     7921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
     7981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
     8041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
     8101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
     8161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
     8221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
     8281 acatcgattt ggaaaggttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
     8341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
     8401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
     8461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
     8521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
     8581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
     8641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
     8701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
     8761 tgttattctt aataattggt ctattatctg ttatttttcc ttagttgttt ttgtctttta
     8821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
     8881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
     8941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
     9001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
     9061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
     9121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
     9181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
     9241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
     9301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
     9361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
     9421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
     9481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
     9541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
     9601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
     9661 cctgttttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
     9721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
     9781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
     9841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
     9901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
     9961 agtaaaggct gcctcacctg ttggaagtac ataa
//
LOCUS       DANKHK010000087         9666 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000087 DANKHK010000000
VERSION     DANKHK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            467..1966
                     /locus_tag="PW109_002075"
     CDS             467..1966
                     /locus_tag="PW109_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2511881.1"
                     /translation="MAASLDVVYSTVLQNGIRKFKYKNSHLKPVSFSDQSGKGAIFAY
                     RSKEHMIEGIGLVITSEEGVIENNNQFTHWTPNVFRYGTYADEARMFTKGHSEDNLRQ
                     INTFFVDFDTLDPNFDYGEIILASHEIGFMPTMILRTPHGFQAFYVLDKPAYVTKKSN
                     FKVINVAKMISKNLRNKFSNDYRFPVDVNANHFGITRMPSEENVLFFEPQYRYSFEEW
                     IDWSMREDADKKAEIKNVFVLPSTQEFRQVDEPWFQLLLKSQKIQGEKGVYGRNNVIF
                     TLSLAYFSSGYEVEACELTMLEFNDRLANPLSDRELTRIICSAYSGKYQAAHREKVVL
                     LCQEWVDANLSEEQLFNKKRGWWKFKKERAVRKYSHSHEWAEDLLSYLNDQSYEEQPY
                     IISTKKELTERLKIPKRSLDKVLKQLREENKIHFRVRAGRNGGIMLASIHGLMRSVIR
                     LKKESRGAYFAAISEAFGKEYTFIQTALKRLIDPNKIGVQTDLFMIDTG"
     gene            2102..2389
                     /locus_tag="PW109_002076"
     CDS             2102..2389
                     /locus_tag="PW109_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2511882.1"
                     /translation="MSINTFVYPYPINVFIISRLGMTVEQFCELHGYKQSTVASWVTR
                     NRTVKTLPCDFLYCLSLSSGWSMDDVYRHLLVLEEKFLKSINPKKKKEQVD"
     gene            2393..2707
                     /locus_tag="PW109_002077"
     CDS             2393..2707
                     /locus_tag="PW109_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511883.1"
                     /translation="MTEEELSVLNRIKEWSVLLKIYYGILFFSFVTVGLVSLLLTLLS
                     LWTNMMLFLYLLLLFFLGLGGVGTAILDDYKWALFSGICRRMKKSATVESYECWKKEM
                     NV"
     gene            complement(2749..2979)
                     /locus_tag="PW109_002078"
     CDS             complement(2749..2979)
                     /locus_tag="PW109_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511884.1"
                     /translation="MWAQYLTNVLNIYMDDQLNEKQNRLNKLNQIVTNWHNLLPSSHL
                     VEGLHGAFQRLSDRNGDRPNNIHIPPVYILKP"
     gene            3398..4018
                     /locus_tag="PW109_002079"
                     /pseudo
     CDS             3398..4018
                     /locus_tag="PW109_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
     gene            4655..5077
                     /locus_tag="PW109_002080"
     CDS             4655..5077
                     /locus_tag="PW109_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2511885.1"
                     /translation="MEIDYKDHGKKPYIINIEDATTGNDNYRTTIWTGSKLQVTVMSI
                     QPGDDIGLEVHEGIDQFIRIEEGEGVCKMGPSKDNLHVQQKVMDDDAVFVPADMWHNI
                     ENTGDKPLRLYTIYAGPDHLEGTVHPTHEDAKNDPNEH"
     gene            5160..5813
                     /locus_tag="PW109_002081"
     CDS             5160..5813
                     /locus_tag="PW109_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2511886.1"
                     /translation="MSDSLFQLEKVGFQANGNQILQDISFTVNKGEVIVFSGPSGSGK
                     STLLKLIGTLLSPTSGKIYYRGTDLQKIDPVEYRKEVSYLFQNASLFDETVQENLSFP
                     ARIREEGFDRERAKKLLERVQIPETYLSKEVKELSGGEKQRIALVRNLMYQPKVLLLD
                     EVTSSLDQENRAIVLDLVRDMMKDQQTTVLAVTHNQEEIDQASRLITIKGGKMEESK"
     gene            5810..6574
                     /gene="fetB"
                     /locus_tag="PW109_002082"
     CDS             5810..6574
                     /gene="fetB"
                     /locus_tag="PW109_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDL2511887.1"
                     /translation="MTNNLEISNLSLLLSSILLIVALLIDYKEKLGLGNDIFIAGIRA
                     VVQLFLIGYVLSYVLRVDNNFLTLAMVLFIVFNASYNAHTRSEGINRSFKISTTAIGV
                     GTALSLLILVFSGVIDWSPSQIVPITGMIASNAMTAIGVTYRSLNSKFTDQRQQVLEK
                     LALGANKKQASMSIVRESVKTGMAPSIDRTKTVGLVSLPGMMSGLIFAGIDPVQAIRY
                     QIVVMFMLISVTAISSFIASYMAYREFYNNRSQLII"
     gene            6882..6959
                     /locus_tag="PW109_002083"
                     /pseudo
     CDS             6882..6959
                     /locus_tag="PW109_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
     gene            7170..7349
                     /gene="yidD"
                     /locus_tag="PW109_002084"
                     /pseudo
     CDS             7170..7349
                     /gene="yidD"
                     /locus_tag="PW109_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            7570..7842
                     /locus_tag="PW109_002085"
     CDS             7570..7842
                     /locus_tag="PW109_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511888.1"
                     /translation="MKKTNFIVVFWLLLSLIAFIVFVLNFASLWQDIAYWIVPSKDLY
                     MDKPEIIRDLIKVVPMIILGIVVFVIGIRQGLKNYYGDYSGDTKQQ"
     gene            7881..>9666
                     /locus_tag="PW109_002086"
     CDS             7881..>9666
                     /locus_tag="PW109_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2511889.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGHYNEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIFQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGRIADV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTSFITD
                     SEFAYLKANFDKYCHYLNNDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGEHAEVDVKSVEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFINNIK"
BASE COUNT         3262 a         1588 c         1969 g         2847 t
ORIGIN      
        1 atatgttgtc aattataatt tatattttat aatttataaa atataaatta taaataagtc
       61 acttaataat ctttacaaat gtttaaaaat agggtaactt tgtagtataa aaaaaaaggt
      121 ttacagcatt aaaaaaaagt ttacacttta aaagaaaatt acataaaaaa aagagacgga
      181 taaaaaagtg aaattgccct cactattcta tctgaatcac ataaaaagcc tatgtgttct
      241 cgctcgtctc agaagttatg taagtggctg acaaccactt atgtaatttt gataagtctt
      301 tcggactgac aatccgaact tacagattta ttctaccatt ttttgacgag caactcaata
      361 tatacacgct tattttgtca ttcatttaag acttctaagc tattgggaga acacaaatct
      421 ttaagattat ttgtgttctc ttttagtttg ggaaggaata acagtcatgg cagcttcttt
      481 ggatgtcgta tattcgacag tattacaaaa tggtatccgt aagtttaaat ataaaaacag
      541 ccacctaaaa ccggtctctt ttagtgatca gtcgggtaag ggggctattt ttgcttaccg
      601 ttcaaaggaa cacatgatcg agggtattgg actagtgatc acttcagaag aaggggtgat
      661 cgaaaacaat aatcaattca ctcattggac gccaaatgta tttcgctatg gaacttatgc
      721 tgatgaagcc agaatgttta caaaaggtca ttctgaggac aatttacgcc aaatcaatac
      781 tttctttgtt gattttgaca cattggatcc aaactttgac tacggagaaa ttatcttagc
      841 cagtcacgag atcggcttta tgcctacaat gattctacgt acaccgcatg gatttcaagc
      901 tttctatgta ctagacaagc ctgcatacgt gacgaagaaa tcaaatttta aagtgatcaa
      961 tgtggccaaa atgatctcaa aaaatctaag aaacaaattt tcaaatgatt atcggtttcc
     1021 agttgatgta aatgccaatc attttgggat cacacgaatg ccttctgaag agaatgtttt
     1081 attctttgaa ccacagtatc gttatagttt cgaagaatgg atcgactggt caatgagaga
     1141 agatgcggac aaaaaagcgg agatcaagaa cgtttttgta ttaccatcta ctcaagaatt
     1201 tcgtcaagtc gatgaaccct ggttccagct gttgctaaaa agtcaaaaaa tacaaggtga
     1261 aaagggcgta tatggtcgaa ataatgtcat attcaccttg tctttggcat acttttcgtc
     1321 aggatacgaa gtagaagcat gtgaattgac gatgttagaa ttcaatgacc gcttggctaa
     1381 ccctttatct gatcgagaat taaccagaat catttgttcc gcttactccg gtaaatatca
     1441 agcagcgcac agagaaaaag ttgtcctatt atgccaagag tgggtcgatg ccaatctttc
     1501 tgaagagcaa ctattcaaca aaaaacgggg ttggtggaaa ttcaagaaag aaagagctgt
     1561 tcgcaagtat tcacatagtc atgaatgggc agaagatcta ctgtcctatt tgaatgatca
     1621 atcttatgaa gaacaaccat acattatttc tacaaaaaaa gagttaactg aacgtttaaa
     1681 gatcccaaaa cgttccctgg acaaagtact gaagcagtta agagaagaga ataaaattca
     1741 ttttcgtgtt cgtgctggtc gtaatggggg tattatgtta gcttcgatac atggattgat
     1801 gcgttccgtt atccggctaa aaaaagaaag ccggggggct tatttcgctg cgatctcaga
     1861 agcttttgga aaagaataca cgtttatcca gacagcactg aaacgcttga tagatcccaa
     1921 taagatcggc gtgcaaacag atctattcat gatcgataca ggctgaaatt aaaaggtgca
     1981 aattgcctat tacattatat ctatgatacg ttccttcttt tctccgcttt gcttgctggt
     2041 cttaagaaag agtagtacta ttattgcaaa cgtaataata tgctgggagg tctttctgac
     2101 tatgagtatc aatacctttg tatatcccta cccgatcaat gtgtttatta tttcgcggtt
     2161 aggtatgaca gtagagcagt tttgtgagtt gcatggctat aagcagagta cagtagcaag
     2221 ctgggtaaca agaaaccgta ccgtaaagac gttgccttgt gattttttgt attgtcttag
     2281 tctttcttca ggctggtcga tggatgatgt ttatcgtcat ttattggtgt tggaggagaa
     2341 gttcttaaag agtatcaatc caaaaaagaa gaaagaacag gtggattgat tgatgacgga
     2401 ggaggagtta tctgttctta atagaatcaa agaatggtct gttcttttga aaatttatta
     2461 tggtattctg ttcttttcat ttgttacagt aggcttagtt tctcttttgt tgactctttt
     2521 atcgttatgg acgaatatga tgttattttt atatttactt ttactgtttt tcttaggatt
     2581 agggggagta ggtactgcca ttcttgatga ttataagtgg gcattgttta gtggaatttg
     2641 taggagaatg aaaaaaagtg caactgttga gtcttatgaa tgctggaaga aggaaatgaa
     2701 tgtgtagagg tatacaaaag agcctagcaa tagatactag gctttttcct atggctttaa
     2761 aatgtataca ggtggaatat gaatgttgtt tggacgatca ccgtttctgt cagataaacg
     2821 ttggaaagcg ccgtgtagac cttctaccaa atgactggat ggcaataaat tgtgccaatt
     2881 tgttacgatt tgatttaatt tatttaatcg attctgtttt tcattaagtt gatcatccat
     2941 gtaaatattt agtacgtttg ttaggtattg tgcccacatt tttaattcga tagagtccac
     3001 atggtctgct acgaaagcta gtttttccaa tatgagtttt gattcttcaa agtaccctaa
     3061 gtccattaag cagtcagata agtttcccaa gcagataggg taatatttct tgtaaaaatg
     3121 gttgaatgag tccatgacat tgaagtcaac agcaagtagt ttttgactaa ctatttgcag
     3181 ctggtcaata gataatgggg tcataaagtc tgcgaaaaat tggaaatcat aagaagtcgg
     3241 gtacgtggct tcttatgatt tgatttataa gagccagggc tttagtgata tcgattttag
     3301 gaatttaagt agtgtcattt gataaaaatt tctcaagcag cattcttaaa tggattagtg
     3361 tcaaattatt gttttcgttt ctaaaacatc cttgtatagc aaagacattc ttaaagaaac
     3421 cgaagggatt ggaaccgagg ccactagagc cagtatcatt gaaaccttga ataaacagga
     3481 ctacatcacg atcagtaaga gtaaaatcta tgtgacagaa aaaggcgaat tgttgtgccg
     3541 cataattgca gaggacgaaa tcgccaatgc cggcatgact gcccaatggg aacgctattt
     3601 gaaaaagatc cgaagccaac aaggcacaca agaagctttt ttaagcagta tcgagcggtt
     3661 tgtgcagcac ctcattgaaa aagtgccaca aaacttccaa gacaaaaaag aaaacattgc
     3721 agatgtagcc ggtcatatgg aacaagaaaa tgtgatgggt acgtgtccga aatgtcaaaa
     3781 cagcgtagta gacaaaggaa aattttatgg ctgttcgggc tacaaagacg gttgcaaatt
     3841 tacgcttcct aaaagatgga gccaaaaagc actcacaaag aaaaatgtcc aagatttatt
     3901 aagcaaaaga gaaacgagtt taatcaaagg gttcaaaagt aaaaaaggaa gcaatttctc
     3961 ggcaaagtta acactgaatg acgaaatgaa attagctttt gaatttccaa aaaaataaaa
     4021 acacaagatc tagaccttgc tccttttgga aaaaaggggc aagcccccaa aatcgcctca
     4081 gtcacccagt tgtaatagcc taaaactgaa taacacttaa ataagtcaag gaacgggtaa
     4141 gtatttttta aaatcatttt aacaagtttt ggacaaaaat tctcatgaaa aaacagaatt
     4201 taaatagcat gataaattag agaagatagt ttaatgtgct tataggaaca ccccactttt
     4261 acagtcacaa tcaatagcct tgctactttc tttcgaaatt gataagagtc taaaggattg
     4321 acaacgcttg atagtgagca taaacttagt gtacgctagt tagtttacaa gtgtaaacta
     4381 aaaaagtatg atgtgtcgtc tctgaaattc tccctgattc aagtcataac cagagtacat
     4441 atcggttatt ttttgattcc atattttttc ctttatgtgc tgaggattaa ggggtaccaa
     4501 ttgtaattgc gcgttatatg gtctttgttt agaatcgctc tccaaaaagc caattttttg
     4561 catagaattt ttagatgtgg taccattaat tagaattatt ataaataaga agtaggtatc
     4621 attgttttag taaaaagact aggaggaatg gaagatggaa atagactaca aggatcatgg
     4681 gaagaagccg tatatcatta atattgaaga cgccaccaca ggaaatgata attaccggac
     4741 gaccatttgg acaggcagta aactgcaagt caccgtaatg tccattcaac cgggcgatga
     4801 catcggtttg gaagtgcacg aaggcattga ccaatttatc cgtattgaag aaggagaagg
     4861 cgtgtgtaaa atggggcctt ctaaagataa cttacatgtt caacaaaaag tgatggatga
     4921 tgacgccgta tttgttccgg cggacatgtg gcacaacatc gaaaacaccg gtgataagcc
     4981 gctcagactg tataccattt atgctggtcc agatcacctt gaaggaacgg ttcaccctac
     5041 tcatgaagac gccaaaaacg atccaaacga acactgaggt acactaggat gaaacaaaag
     5101 ccctgcaccc gtatggatgg tccactggtg cagggctttt tataaaggag agaagtggta
     5161 tgagtgattc attgtttcaa ttagagaagg ttggttttca ggcaaacgga aaccaaatcc
     5221 ttcaagatat ctcttttaca gtaaacaaag gggaagtgat tgttttttct ggtccttctg
     5281 gaagtggaaa aagtaccctt ttgaaattaa ttggtacgct gctttcccca acttccggaa
     5341 aaatttatta tcgaggtacg gatctgcaaa aaatcgatcc tgtggagtat agaaaagaag
     5401 tctcttattt atttcaaaac gcttctctat tcgatgagac agttcaagag aacttgtctt
     5461 ttcctgcccg cataagagaa gaaggatttg atcgggaacg tgcaaaaaaa ttgttagaac
     5521 gagttcaaat acctgaaact tacttatcga aagaggtcaa ggaactatca ggaggggaaa
     5581 aacaacggat tgcattggtc cgaaatttga tgtatcaacc gaaagtgttg ctcctggatg
     5641 aagttaccag ttcattggac caagaaaaca gagcgattgt ccttgattta gtccgagaca
     5701 tgatgaaaga ccaacaaact acggtgttgg ccgtcactca caaccaggaa gaaatcgacc
     5761 aagcttctcg tctgattact atcaaaggtg gaaaaatgga ggaatcgaaa tgacaaacaa
     5821 tttggaaatc agtaatcttt ctttgctttt atcctctatc ctcttgatag ttgccttgtt
     5881 gattgattac aaagagaaat tgggattagg aaacgacatt tttattgcgg gaattcgcgc
     5941 tgtcgttcaa ttattcctta ttggatatgt attgagctac gtattacgag tggataacaa
     6001 ctttctcact ttagcgatgg ttttgttcat tgtgttcaac gcttcttata acgcccatac
     6061 acgcagtgag gggatcaacc gctcttttaa aatttcaacg acggctattg gagtaggaac
     6121 agccttgtcc ttactcattc ttgttttttc aggagtcatt gactggtccc cttcccaaat
     6181 cgttccaatt acaggcatga ttgccagcaa cgccatgact gcgattggag tgacctatcg
     6241 gtctttgaat tcaaaattta cggaccaacg ccaacaggta ttggaaaaat tggcattggg
     6301 agccaataaa aaacaagcgt ccatgagtat tgtaagagaa agcgtcaaaa cgggcatggc
     6361 tccttctatt gaccgaacaa aaacggttgg acttgtcagc ctaccaggta tgatgtcagg
     6421 gttgattttt gcgggaattg atcctgttca agcgattcgt taccaaattg ttgtgatgtt
     6481 tatgttgatt tcagttactg cgatttcttc gtttattgct agttatatgg cttaccgtga
     6541 gttttataac aatcgcagtc aattaattat atgatttggg atgatacact actcttaaaa
     6601 ccagtggtta caattttatg tagttaaaag aagacctcac gagaataatt gcaaaaagaa
     6661 cttcattttt taaggaagaa ggatattttt gtcatcgaat ctttggaacg actagggcga
     6721 aactatgatg aaatttttta tatagtgcag caactggatt aaaaggatat cggactgatt
     6781 gttttgaaca tgcctatcct caaccaatga gaactttcga cgaaatcatg ttctagaaag
     6841 aaatttctat ccaataagaa ttccaaatca tggacactat tatcaaagat gataatttga
     6901 caatgattgc tagagaagtt gaatcaacat taaaagaagt agtggacgct gctaaataaa
     6961 tataaaaaag actttctcgt gaactaaaat ggacccctat agaggacact aaaaaaagtg
     7021 tcctctatag tttttttgcc catagggagg aaggtcatgt aactttatcc actagctctt
     7081 aatacaagat tcggaaatac cgacatatta tggtcgtttt tattcttttt gaaaaatgct
     7141 ttacaaaaaa tatgcatggg ggtatataaa tgaaaaatat cattttatta agtattataa
     7201 aaggttatca aaaaggaatt tcgccgttgt ttccaccgag ttgccgttat tatcccacct
     7261 gctcgcacta tgctgttgat gcgattgaaa aacatggagc agcaaaaggg tctctaatgg
     7321 gagcagcacg tattatgcgg tgtcatccat tgtctgtgtg gtgttaatca ttggtagttt
     7381 taaaataagg aaacaaagta ggggaacggc tttatttttg cttttattag gaatagttgg
     7441 tattatcgca agtctgtatg gtttcttttt tacaatgttt tttgggatca acttctagga
     7501 actacttgga tttagaaaaa tatcttaaaa aaggaagttt aaacaaaaat agaaaggagt
     7561 gtgtgaggaa tgaaaaaaac aaattttatc gttgtgtttt ggttattgct gtctttaatt
     7621 gcgtttattg tttttgtatt gaattttgct agtttatggc aagatattgc ttactggatt
     7681 gttccaagca aagaccttta tatggacaaa cctgaaatca taagagattt aatcaaggtt
     7741 gttccaatga ttattttagg aatcgtagtt tttgtcattg gtattaggca aggactgaaa
     7801 aattactatg gcgattattc aggtgacacg aagcaacaat aactaaagct atctaaaatt
     7861 aggaaaaaag gagagaatga atgagtacgg ttattttagc tgaaaaacca agccaggcat
     7921 tagcctacgc aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag
     7981 acccactatt tacagatgaa acgtttatca cctttggttt tggtcattta gtcgaattag
     8041 cagaaccagg tcattataac gaaaagtggc agaactggaa acttgaatct ttgccgattt
     8101 ttcctgatcg atacgatttt gaagtcgcaa aagataaggg aaagcagttt aaaattgttg
     8161 cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac agagaaggtg
     8221 aaaatatcgc ctggtcgatt atccataaag caaatgcctt ttcaaaagat aaaacattta
     8281 aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt caaaatttgc
     8341 aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa attgccgatt
     8401 ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag aagggcgtac
     8461 aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt tttcagcgcc
     8521 aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct agtataaaag
     8581 taaaccaagg ttcgtttaag ggcgttctaa gccccacaca gcgttttaaa acccaagagg
     8641 agcttttagc ttttgtttct tctaaacaag ctaaaatagg caatcaagag ggcagaatcg
     8701 ctgacgtgca aaccaaagag aaaaaaacga atagtcctag tctgttttct ttaagtagtt
     8761 tgcaatcaaa agtcaatcag ctttataaag cgacagcgag ccaaacttta aaagctatgc
     8821 aaggactgta tgaagcaaaa ttattgagtt atccaagaac agacacgtca tttattacgg
     8881 acagcgaatt tgcgtattta aaagcaaatt ttgacaagta ttgtcactat ttgaataatg
     8941 atcttgaaat ggttcaaaca gagcctagaa agcgttatgt ggacggtagc aaggtacagg
     9001 aacaccacgc cattatccca acaaaacaag tacctaccga atctgcatta gcaaaaatgg
     9061 acgatttaca acgaaaaata tatgctttag tcgttaaaac gaccgtcgcc atgtttttac
     9121 ctgattattt gtatgaagaa accaagatac aaacgaaagt agctgatttg ctgtttcaat
     9181 cgatcggaaa gacaccaaaa caagaaggtt ggaaaattct tttcaaacag caaaccaaag
     9241 aagaaaaaga ggacgttcaa acgttaccac tcgttatcat tggcgaacat gccgaagttg
     9301 atgttaagag tgttgaaaaa gaaacgcaac cgccaaaagc ttttacagag ggtacgcttt
     9361 taaccgctat gaaaacggcg aataaaacag ttgatgatga agaagcaatc aagattttac
     9421 aagaagtcga ggggatcgga acagaagcga caagagcaag cattattgaa gccttgaaac
     9481 aaaaagaata tatccaagtc attaaaaata aacttgttgt gactgaaaaa ggaaaattat
     9541 tgtgccaggc agtcgaaagt cagcacctct taacgagtgc tgaaatgacg gctaaatggg
     9601 aaacgtattt aaagaagatt ggtaaaagag aaggcaatca agaaaacttt attaataata
     9661 tcaaga
//
LOCUS       DANKHK010000088         9471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000088 DANKHK010000000
VERSION     DANKHK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(368..1486)
                     /locus_tag="PW109_002087"
     CDS             complement(368..1486)
                     /locus_tag="PW109_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511890.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="PW109_002088"
     CDS             1957..3264
                     /locus_tag="PW109_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2511891.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="PW109_002089"
     CDS             3383..4582
                     /locus_tag="PW109_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2511892.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="PW109_002090"
     CDS             complement(4609..5877)
                     /locus_tag="PW109_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2511893.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="PW109_002091"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="PW109_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="PW109_002092"
     CDS             complement(7094..7597)
                     /locus_tag="PW109_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2511894.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="PW109_002093"
     CDS             complement(7720..8484)
                     /locus_tag="PW109_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2511895.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="PW109_002094"
     CDS             8591..8866
                     /locus_tag="PW109_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2511896.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2832 a         1970 c         1686 g         2983 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg c
//
LOCUS       DANKHK010000089         9354 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000089 DANKHK010000000
VERSION     DANKHK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            44..238
                     /locus_tag="PW109_002095"
     CDS             44..238
                     /locus_tag="PW109_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511897.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            507..806
                     /locus_tag="PW109_002096"
     CDS             507..806
                     /locus_tag="PW109_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511898.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(903..1337)
                     /locus_tag="PW109_002097"
     CDS             complement(903..1337)
                     /locus_tag="PW109_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2511899.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1569..2279)
                     /locus_tag="PW109_002098"
     CDS             complement(1569..2279)
                     /locus_tag="PW109_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2511900.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2269..3129)
                     /locus_tag="PW109_002099"
     CDS             complement(2269..3129)
                     /locus_tag="PW109_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2511901.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3134..3772)
                     /locus_tag="PW109_002100"
     CDS             complement(3134..3772)
                     /locus_tag="PW109_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2511902.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3787..4086)
                     /locus_tag="PW109_002101"
     CDS             complement(3787..4086)
                     /locus_tag="PW109_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2511903.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4129..5613)
                     /locus_tag="PW109_002102"
     CDS             complement(4129..5613)
                     /locus_tag="PW109_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2511904.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5633..6094)
                     /locus_tag="PW109_002103"
     CDS             complement(5633..6094)
                     /locus_tag="PW109_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2511905.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6112..7179)
                     /gene="ulaG"
                     /locus_tag="PW109_002104"
     CDS             complement(6112..7179)
                     /gene="ulaG"
                     /locus_tag="PW109_002104"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2511906.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7397..8167
                     /locus_tag="PW109_002105"
     CDS             7397..8167
                     /locus_tag="PW109_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2511907.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8440..9267)
                     /locus_tag="PW109_002106"
     CDS             complement(8440..9267)
                     /locus_tag="PW109_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2511908.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2905 a         1925 c         1475 g         3049 t
ORIGIN      
        1 tatttgaata ttaactaaaa acagcaagaa agaagaggat caaatgtatt atgtagaagt
       61 aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt atcagtaacg aatatccttt
      121 acttggttca tggaaagaag ctgctccttt ttccaaacca tgtgccataa aaataaagaa
      181 cgagctagaa aaagaactga cttgtggtaa agctgttgta gatatcatcg aaaagtaaat
      241 ccaatgtata gaaaggaaga tcacacatga aaagcgacgt acaaattgct aagattatag
      301 aattagaaga agaagacgca gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata
      361 tgattatttc aatatgtatc ctggaattgg agatctcgta gagctatatc aagatgaatc
      421 aaatatactt gtcattcatc atgaaatata gagacttata aaattgttta tttttataat
      481 gtatcagtat agaaaggaaa taaaacatgg attatactgt tgaatttgac agtgaactac
      541 ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc cacaaagtga
      601 cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt tcggcaaaag
      661 gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca tttgctttaa
      721 aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt gtatttgata
      781 aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag tgatactaag
      841 tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta cgttattata
      901 tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac ataagaaata
      961 ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt tgttttctca
     1021 gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt ctccaaagct
     1081 tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat gaggatatct
     1141 tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat aatcttccct
     1201 tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa ccttgcagtg
     1261 tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc atcgatcata
     1321 agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct aataatacag
     1381 ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct cgattcctta
     1441 aaattcctct ttccttttag aatgcatact ttttgataaa taaaaaggtt gtgataaatt
     1501 ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct acccttaccc
     1561 tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata atgtttatct
     1621 agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc tttggccatc
     1681 agacaaattt catctaagat caaactgttt tctactgctt tcataggtga atccccccat
     1741 gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc taattttctt
     1801 tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc tcttacttct
     1861 tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc atgagtcgtg
     1921 ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt ggaatgagta
     1981 tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct
     2041 gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt taccaccatt
     2101 tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat gactcctagc
     2161 tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta tcgcggcaat
     2221 gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc acaagacatc
     2281 atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga tttctcttcg
     2341 aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat aatctagacg
     2401 tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag gaacatcacg
     2461 aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga tagcagtgat
     2521 ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg cggacaaatt
     2581 gcctaggtct ggatatactt gtagaaaagg tgaatgaatc tgttctttga tttctaaaaa
     2641 ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa tgatgccata
     2701 tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc tggaattcat
     2761 tgatttctct tcataataaa catcgtatcc agctatttgg attactttga cgcttagatt
     2821 atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct tttctggatc
     2881 agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga tacgtacacc
     2941 ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat ctaatcgtgc
     3001 taaccgctca tccgtctcat caatcgacat ctcgataaaa tcgaagtcca tttcttttac
     3061 aagggaaaaa cgttctttcc atgaaatatc tttgggtaac gctttttcat aaatcccaat
     3121 ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt tgtgcggcct
     3181 tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac acatcgatcc
     3241 cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca ttaaagccta
     3301 gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca ccagataaca
     3361 gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc caatccatgg
     3421 cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg
     3481 ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct gctcctgctt
     3541 ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca gcgactagtt
     3601 ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa
     3661 gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca tctcctaatg
     3721 catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc atcttagttc
     3781 cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac tgatttcttt
     3841 gactgacatc acatttttga taccgataac tgtaactcct tttttctctg cctgagtaaa
     3901 catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg aactttttcc
     3961 ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt tcattgcttt
     4021 ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg atactaaaat
     4081 tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt atactctctg
     4141 cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccatcag gatttttccg
     4201 atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc cagcataacc
     4261 taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga acatcccgat
     4321 ataaccaccg tatgttgaca tcccaatcca tgtagcgaat aatgctgaac cgcctacttg
     4381 tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc ggttattggc
     4441 ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta tgacgatcgt
     4501 tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa
     4561 gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc
     4621 tggaacagct cctggtaaaa tcgtgtttga gattccttgg aaagattgag ttagttcatc
     4681 gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat ttaaagaagt
     4741 cgtcataatg tagaataaga aactctgccc ttcttgcatg aattgcgctt gaatcaaata
     4801 atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta acgaagtagc
     4861 aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt caattttttt
     4921 gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact cagctaattt
     4981 tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg caccttctgt
     5041 caattcctgc gtgattccta ctgttaagtt agaagctact gcccaataac aacctaaaat
     5101 cagtcccata aaaatcaata cttgtatcct acctagatca ggaaagcaaa acagtaaaat
     5161 ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag
     5221 tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa tcagtaataa
     5281 aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat ccacagcatt
     5341 ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct ttagtccaac
     5401 caagattggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa taaagtaacc
     5461 gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca gcaaaatata
     5521 gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat ttgttgcaaa
     5581 atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc aattaattat
     5641 attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga cgctcaacca
     5701 atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt tcttctggat
     5761 cttttgctgc gagtgtaaaa aacaaactag ccgttttctc ctcattctcc ccttcacttt
     5821 caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa acaccatcac
     5881 ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa ggaccgtatt
     5941 tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc ttatcgatca
     6001 atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta tctgggtaat
     6061 gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt cttataaaaa
     6121 cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc gataaaaatt
     6181 aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt gccagatata
     6241 aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag tgatctcttt
     6301 tggatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta cttttgcatt
     6361 caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta tccctcgtgg
     6421 gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat gtttggcaaa
     6481 taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg tttcgaataa
     6541 atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata cctcttctgg
     6601 atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta cattgatgtc
     6661 tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag gaattcccca
     6721 tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag gacaattttg
     6781 atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg taacgattaa
     6841 agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac gtaaattagg
     6901 ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca tcatgccatt
     6961 cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg ttccttcatg
     7021 acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc caggatccac
     7081 tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg tttctactaa
     7141 ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc ccccatatcg
     7201 ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatgaaaac ccttttatat
     7261 gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca gtataataaa
     7321 gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc aagatgtccc
     7381 tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat
     7441 tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg aatcccttga
     7501 tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa acgagatcat
     7561 acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg atcaatctgg
     7621 tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta acgattatga
     7681 tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc ttcaatctaa
     7741 ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca aagataattg
     7801 tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag tgggcgacat
     7861 cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct gtagcggagt
     7921 cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca acgaaatgat
     7981 ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa tcggactgac
     8041 atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg ataaattttg
     8101 ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc aagtagattc
     8161 attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga ataacaaatt
     8221 tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa gatgtatcca
     8281 tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt tgcctaaata
     8341 ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct tttttcgaat
     8401 aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt cgttttcaat
     8461 cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt ccatatatct
     8521 ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat aaagcaagta
     8581 ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct cacataattg
     8641 gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat aatatacaat
     8701 gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac gctccacata
     8761 aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt ttgcagctgc
     8821 ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa aataaaataa
     8881 tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa ataaaatttg
     8941 atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa ttttataaga
     9001 ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt tcctgatttt
     9061 gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt tgcaaagcgc
     9121 acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga cagttgcttg
     9181 ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc ggtattcttt
     9241 tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa attatttctc
     9301 ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa caga
//
LOCUS       DANKHK010000090         9273 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000090 DANKHK010000000
VERSION     DANKHK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(4..672)
                     /locus_tag="PW109_002107"
     CDS             complement(4..672)
                     /locus_tag="PW109_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2511909.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            806..1093
                     /locus_tag="PW109_002108"
     CDS             806..1093
                     /locus_tag="PW109_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511910.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1509..3047
                     /locus_tag="PW109_002109"
     CDS             1509..3047
                     /locus_tag="PW109_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2511911.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3044..4009
                     /locus_tag="PW109_002110"
     CDS             3044..4009
                     /locus_tag="PW109_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511912.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3996..5318
                     /locus_tag="PW109_002111"
     CDS             3996..5318
                     /locus_tag="PW109_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2511913.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5381..5485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5559..6302
                     /locus_tag="PW109_002112"
     CDS             5559..6302
                     /locus_tag="PW109_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2511914.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6317..7309
                     /locus_tag="PW109_002113"
     CDS             6317..7309
                     /locus_tag="PW109_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2511915.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7321..8058
                     /locus_tag="PW109_002114"
     CDS             7321..8058
                     /locus_tag="PW109_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2511916.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8048..8770
                     /locus_tag="PW109_002115"
     CDS             8048..8770
                     /locus_tag="PW109_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2511917.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8854..>9273)
                     /locus_tag="PW109_002116"
     CDS             complement(8854..>9273)
                     /locus_tag="PW109_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2511918.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3031 a         1603 c         1962 g         2677 t
ORIGIN      
        1 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
       61 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
      121 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
      181 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
      241 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
      301 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
      361 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
      421 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
      481 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
      541 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
      601 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
      661 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
      721 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
      781 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
      841 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
      901 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
      961 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
     1021 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
     1081 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     1141 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     1201 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
     1261 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
     1321 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
     1381 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
     1441 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
     1501 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
     1561 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
     1621 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
     1681 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
     1741 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
     1801 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
     1861 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
     1921 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
     1981 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
     2041 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
     2101 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
     2161 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
     2221 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
     2281 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
     2341 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
     2401 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
     2461 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
     2521 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
     2581 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
     2641 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
     2701 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
     2761 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
     2821 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
     2881 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
     2941 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
     3001 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
     3061 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
     3121 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
     3181 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
     3241 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
     3301 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
     3361 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
     3421 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
     3481 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
     3541 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
     3601 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
     3661 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
     3721 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
     3781 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
     3841 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
     3901 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
     3961 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
     4021 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
     4081 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
     4141 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
     4201 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
     4261 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
     4321 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
     4381 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
     4441 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
     4501 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
     4561 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
     4621 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
     4681 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
     4741 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
     4801 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
     4861 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
     4921 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
     4981 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
     5041 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
     5101 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
     5161 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
     5221 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
     5281 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
     5341 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
     5401 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
     5461 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
     5521 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
     5581 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
     5641 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
     5701 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
     5761 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
     5821 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
     5881 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
     5941 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
     6001 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
     6061 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
     6121 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
     6181 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
     6241 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
     6301 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
     6361 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
     6421 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
     6481 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
     6541 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
     6601 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
     6661 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
     6721 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
     6781 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
     6841 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
     6901 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
     6961 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
     7021 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
     7081 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
     7141 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
     7201 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
     7261 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
     7321 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
     7381 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
     7441 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
     7501 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
     7561 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
     7621 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
     7681 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
     7741 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
     7801 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
     7861 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
     7921 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
     7981 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
     8041 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
     8101 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
     8161 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
     8221 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
     8281 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
     8341 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
     8401 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
     8461 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
     8521 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
     8581 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
     8641 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
     8701 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
     8761 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
     8821 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
     8881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
     8941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
     9001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
     9061 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
     9121 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
     9181 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
     9241 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DANKHK010000091         9175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000091 DANKHK010000000
VERSION     DANKHK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            54..2831
                     /locus_tag="PW109_002117"
     CDS             54..2831
                     /locus_tag="PW109_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2511919.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2912..3331
                     /locus_tag="PW109_002118"
     CDS             2912..3331
                     /locus_tag="PW109_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2511920.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3343..3813
                     /locus_tag="PW109_002119"
     CDS             3343..3813
                     /locus_tag="PW109_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2511921.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3830..4609
                     /locus_tag="PW109_002120"
     CDS             3830..4609
                     /locus_tag="PW109_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2511922.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4596..5417
                     /locus_tag="PW109_002121"
     CDS             4596..5417
                     /locus_tag="PW109_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2511923.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5434..6483
                     /locus_tag="PW109_002122"
     CDS             5434..6483
                     /locus_tag="PW109_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2511924.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6496..7500
                     /locus_tag="PW109_002123"
     CDS             6496..7500
                     /locus_tag="PW109_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2511925.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8133..9152
                     /locus_tag="PW109_002124"
     CDS             8133..9152
                     /locus_tag="PW109_002124"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2511926.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3056 a         1536 c         1942 g         2641 t
ORIGIN      
        1 tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata
       61 agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag
      121 aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa
      181 acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt
      241 ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca
      301 cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa
      361 aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa
      421 tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg
      481 gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc
      541 tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac
      601 ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata
      661 aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct
      721 taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta
      781 tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag
      841 agattactag tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac
      901 ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg
      961 aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa
     1021 caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag
     1081 caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc
     1141 aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta
     1201 cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg
     1261 ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt
     1321 gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca
     1381 gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc
     1441 gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt
     1501 actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac
     1561 taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc
     1621 aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga
     1681 ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag
     1741 cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt
     1801 tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga
     1861 ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat
     1921 tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac
     1981 cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc
     2041 aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat
     2101 ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg
     2161 gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc
     2221 agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat
     2281 tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata
     2341 tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat
     2401 ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta
     2461 atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg
     2521 gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc
     2581 ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa
     2641 tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat
     2701 ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt
     2761 ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag
     2821 aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta
     2881 gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca
     2941 tggaaaattt gcttcaggga tattagattc attagagctg atctatggga aaaatcattc
     3001 tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca
     3061 gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag
     3121 tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt
     3181 gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt
     3241 tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct
     3301 caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga
     3361 gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca
     3421 gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc
     3481 aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc
     3541 gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag
     3601 gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc
     3661 aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag
     3721 gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca
     3781 gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca
     3841 agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc
     3901 gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa
     3961 aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg
     4021 tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac
     4081 agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt
     4141 gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa
     4201 ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg
     4261 tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat
     4321 cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg
     4381 gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc
     4441 accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt
     4501 agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact
     4561 accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca
     4621 gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt
     4681 cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga
     4741 aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt
     4801 ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag
     4861 aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga
     4921 tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca
     4981 caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt
     5041 tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct
     5101 taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag
     5161 gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca
     5221 atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg
     5281 atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga
     5341 aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt
     5401 ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat
     5461 gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat
     5521 atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta
     5581 gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt
     5641 tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa
     5701 agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct
     5761 gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt
     5821 gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt
     5881 ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa
     5941 atagaacaag actacatgca acagttgcgc aaaataatca accatttacc agcgattatt
     6001 caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc
     6061 gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact
     6121 gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac
     6181 ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa
     6241 cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc
     6301 acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg
     6361 tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa
     6421 ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta
     6481 taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc
     6541 gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt
     6601 gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact
     6661 tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact
     6721 actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca
     6781 actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga
     6841 tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca
     6901 gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat
     6961 ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg
     7021 ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga
     7081 cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat
     7141 gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag
     7201 tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc
     7261 gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc
     7321 aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc
     7381 ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg
     7441 gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt tgattattag
     7501 gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat
     7561 ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt
     7621 caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt
     7681 actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt
     7741 tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa
     7801 cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta
     7861 tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt
     7921 agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat
     7981 aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta
     8041 aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg
     8101 aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc
     8161 ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta
     8221 tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt
     8281 ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag
     8341 tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac
     8401 aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag
     8461 aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa
     8521 gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata
     8581 tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt
     8641 tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg
     8701 cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag
     8761 atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg
     8821 cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta
     8881 cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata
     8941 agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag
     9001 cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata
     9061 atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg
     9121 gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DANKHK010000092         9107 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000092 DANKHK010000000
VERSION     DANKHK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            738..4392
                     /locus_tag="PW109_002125"
                     /pseudo
     CDS             738..4392
                     /locus_tag="PW109_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            4394..5125
                     /locus_tag="PW109_002126"
     CDS             4394..5125
                     /locus_tag="PW109_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2511927.1"
                     /translation="MKNKSLFIKITLLLSTLLVGIFTGQQAFADDGTIDIVIHKQLWD
                     QAPDATIQNTGAADQDTKDPIEGVGFTIYDITEDYYKLVKGSDETTTIQMIQENVADY
                     QKIIREQGLTDEKGTIKFTQLPKSSGGKNAVYLIVETALPDPEKAKVEKAAEPLVVAL
                     PVYEMNTDGKTYTDKELATVHLYPKNVGTIVEKPVTPTKPKKPSKPKKRLPQTGEAKS
                     FLGILGILLIGTAIILWRKHSVRNN"
     gene            5190..6677
                     /locus_tag="PW109_002127"
     CDS             5190..6677
                     /locus_tag="PW109_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2511928.1"
                     /translation="MKKLWKTLFAALLVIPLFAGVFGAKTVSAAEATTTNVTINKRIW
                     KDGEAPAQGSMQNTGEEMDFGGDPLEGAGFTAYDVTDAYLALIADGKSQAEATKEIAD
                     NASDYTTVAKAEQKTDANGQTTFEGLALKDGDKDKVYMFVETSTPGNVSVTTKAAPIV
                     LAMPIYTFKDGKYSDTINTNVQVYPKNETKTDKKEVANLDKFTEVKDADGNVLYHNLT
                     TGDEIEYKLTLNIPADILEDGVTYSVTDTPTAGLAYVKDSFAATGLVAGTDYELAEDS
                     ATGGFTVTLKQSENVGKLAGSQLIATYKMKLTAEVNPDDLVNNSAQVTIGNKPQDKIT
                     PPTQFGTGGYKFVKKDSQSGATLSGAEFVVKQDSNFAIFEDAKNTKGEYIFKEWTTDE
                     AKATKIVSDDKGELKVIGLKNGDYQLEEKATSSDKYVLLDEDVDFTVEHGQYGSQELK
                     SVLNTPKGLLPSTGGNGIYAFLLIGAALMIGAYAWFKKSKTQAEV"
     gene            6764..7960
                     /locus_tag="PW109_002128"
     CDS             6764..7960
                     /locus_tag="PW109_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2511929.1"
                     /translation="MDQRQKNERINLILKLLMAILFTTGAIIFSYPFLANAVNSYYDQ
                     KMMEKNLAEMAATNTKEQAKRHQEMAEKNKELAESKNMTNIPGMGLVEDPFENEVDDA
                     KDPGKAYYEEHTVGAIYIPKISVSLPLFDETNAALLEKGATILQGTSYPIGGNSTHSV
                     ISGHSGLTERKLFTDLEKLVIGDDFYLTIAGEKLAYAVDDIQIVLPSDIDKVVIQPGR
                     DLVTLLTCTPYGINTHRLLVTGHRVPYVEEMAEEVTSTKKAVERRFRLYLLLIPLFFA
                     MIFYWMYRKFVYYQSGKHSYDFCFYLLENGQPKAGVTFTLVRKKRWATDVTSQPVAVS
                     QVDGWVSFPEIRGGRYYAKAIDGSTKPVKGKVRRLKDRQFVLSRVTKKKHGKKVTYYL
                     ENGAKK"
     gene            7957..9078
                     /locus_tag="PW109_002129"
     CDS             7957..9078
                     /locus_tag="PW109_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2511930.1"
                     /translation="MKKNRQVISQIAIILIFMVGILTMLYPFYVDALNNVIDQVRVDR
                     YLKESQKEFEAERKRLAEENNKLSDSGLAPGADPFANPNGGTVSAAYYKKHLIGTINL
                     PDLAIELPLFDTTNDDLLEQGATVLDGTSFPVGGASTHAVISAHRGLPERELFTNLPE
                     LKNGDIFLLNVLGETLAYEVFDSQVVTPDQTSVLKIEPGQDLVTLMTCTPYMINSHRL
                     LVTGKRVPYTPAAEKKQVKGDRFRKLKQITILAGTALLILAAIYQLYHVIARYRLRKV
                     RFDFTVCLEGVAEHTPIALYDKKGKKALRRNGKAYQELTDQTGQVTFTDLPGDCYRLK
                     LGKSWLVQFGLKKKKRPSKIWKINKKKVMLKEERMLKVK"
BASE COUNT         3057 a         1644 c         1995 g         2411 t
ORIGIN      
        1 aacagaacca ttttaggaat ttgattagtg tcatttgata aaaatttctc aagcagcatt
       61 cttaaatgga ttagtgtcaa attattgttt tcgtttctaa aacattcttg tatagcaaga
      121 tagcttgttg aacatcatga tgttcaacaa ggttgtaacg tttttttgag tgttgttgtc
      181 ttcggatatc gaattcaaac aatcactact tatcactaac aagtcaatta tcagattgaa
      241 cagaaaagtg gattgacagc tgaagcagtt gcagagctca gagattactc aaataaacaa
      301 tttcaaggct agtatatgat acaaaattca tcattagggt aaaaagaagt gtttggagat
      361 acggtacctt atacgataaa agcgacctat ccatttttat ttctgctaga caatatctct
      421 gtgaaaatcg gaattgaggg gtaagtgcct tctcaagtga gttgaagata ttggaagaga
      481 tttttcctta gtgttacggc tcgttttcag tttagtcgtg ggtgcgcttc ttcttagagt
      541 agtcttttat tatcaagaca tcataaaagt tgcatggaaa cagtggcgga tacagcaaag
      601 cataggaatg tgaaagagat aataatagta ttatctctat aaattgttta taatttagat
      661 ggcagtatca aatcttaggt agtgctattt aaattttgtt taagaataga tatcatgttt
      721 tttagaggag ggaagaaatg aagaagaaga aatcggtctg gtttatgatg gttgctattt
      781 tgatgggctt tatcccgcaa atcttgactg cttattcgaa tatcgcacag gctatagaaa
      841 ctcagccgga taaaatcgta gatcaatcag gtttaaaagt tagttcctct gtttcatcag
      901 atggaaattc tttaaattgg aaattacagt atgaaaaaga tgtcgcaagt gatggcaatg
      961 atcaagcact gaaattcaaa gtgacggctg atggagaagc aatagctttt aatgaagatg
     1021 ccagttttgc tactaatgat gaccaatggt ttgcggaaaa agatttttcg aaacaatcgc
     1081 agggttctct ttcatttaca acggtgctaa atgtctccaa ggtagctctt gaaattcaag
     1141 ccgatgcaac aaaaacggat gaaacacaag ctgtcactat taacaagaat attttgacta
     1201 gtgcggtaga aggtccgcat gcgttgaaat taccagcagt ggctacacaa aaaacgacag
     1261 aagtaagtgt tacttctcag acaacagctg aagaaaccac tagtagccaa gcgaccgaaa
     1321 cgacgacaca aacggaagaa gccagtgaag caacgtcgtc tgagataaat gtctcgccgg
     1381 caattgtaag cgcaaacaat cttgaatatc ttagtggaat aagtgcattt tcttccatta
     1441 attacagcaa tattgcgcca gaatatacga aagatgctac aaaaggaact ttttcgacta
     1501 atagctggca accagctggg caaacaaatg tgatcaacca ccaaggtaac aaggaacgca
     1561 actttgccac ttgggatggt gtaactagct ggaatggtga tccatcagac acaacacatt
     1621 cttatatcca gtatggtgaa aaacaaacag attttgccat tcggaagtat gccaaagaaa
     1681 cgagtgaacc agggctttac gatgtatatt tgaatgtaaa aggtaatgag caacaagata
     1741 tcaaaccagt tgatattgtt ttggtagtgg atatgtcagg aagtatggaa tcgagtcaaa
     1801 gtaacggctg gaatgatcgt gctggtgcag ccagaaatgg tgtgaaaaat ttcttgcaaa
     1861 ccattaaaga cgcaggcatt ggggattacg tcaatgttgg tttagtcgga ttttctagtc
     1921 caggatatgt aacagggcct aacggttatc ttactgtacc gataggtaaa gcttcggata
     1981 ctagccacat aaatgcaatc aatgatgctt taaaaccaaa atttacagga ggcacttaca
     2041 ctcaaatcgg gatcgagcaa ggtcagcaga tgcttgcggg aagctccaat gaaaacaaaa
     2101 tgatgatcgt attgaccgat ggggttccta ctttttctaa gaaggtaacc gctgcgcaaa
     2161 cgattgatgg gactacctat gctactaaat ttggtaatac attggatgaa ccacgcaata
     2221 cctctaaact caatagtagt tatgaggttg gttcttgggg aaatagaaca aatattaata
     2281 gtacttggcc ggccacatta ggagcagcta aaatcgccaa agatgcgggc ttaactatcc
     2341 acacgctggg aattcagctg agtaaggatg gaaatttttt aactgaacaa caagttcgcg
     2401 atagagcaag tcttatcgct acaccgggta aatacaaaga tgctgaaaca acaaacgatg
     2461 tttctgatta tttgaatgaa caagctaaaa atgtagtgaa atctttcaac accatagtca
     2521 atggttcgat caatgatcct ttgggtgatc agttctctta tgaaggcact ccaactgtga
     2581 agagcatcag tacaggggat agtgctgtaa cgaatttacc gaccgtaaac cagtcgaatg
     2641 gtaaagtgac cactactgag ttgaacttag gcaaagatca agaaattcag atccattatc
     2701 aagtgcgtat caatacagaa gataaagatt tcacgccgga aaaatggtat ccgatgaatg
     2761 gtcaaacaac tttgacacca aacggagaca atccggataa taaagtagac tttggcgttc
     2821 cttccgcaaa ggcaccgggg gtgaaacttg atctgaaaaa gatttgggaa gaatacgata
     2881 aggatacgag taaacgtccc gattctcttg atttcacctt gaaccgaaaa gataccacag
     2941 agagcaattc ttggaagcaa ggttttattc gtctttctaa aagcgatgtt gctacagata
     3001 ctaatgtctg ggaaaagacc aatattgaaa aagtagccga aacacaagga gctactgaat
     3061 cgctttggtt gccacaattc aacaacaaag ggaaagattt tgcttatagc tttgaagaag
     3121 caacggtcaa tggctatgaa tcttcaagta atgctgctac aaccatttgg accaataaaa
     3181 aaatctacac accacttgct ttagagatca ctaaaatttc tgatcaagga aagacacctc
     3241 ttaaaggagc agtctttaaa ctaagtggag gtagtttgcc aaatgcaggc gttgaactta
     3301 aggacaacgg ggatggaacc tatacgttgt ctgatgataa gtacaagctg caattaaata
     3361 ctgagtatac attgacagaa gttacaccac cagctggaca tacggctact acaactagtt
     3421 ggaaagtcaa cgttagtgca gaaggtaaag taaccatcaa cgatgctgta ccgggtattg
     3481 aaatcaaaga caacactatc aagtacacca ttgaaaatga gtttaccaaa cgtcctttat
     3541 tggttgaaaa atataataaa gatagtggta aaatattaga cgatgcgaag ttcacgttga
     3601 agcaatatga cgataagtgg acgaatgatg gtaaagttgt cggtgatgat ttgatcggaa
     3661 atgatgccaa atcttttgct agcctggcac caggctatta cagcttggaa gaaacgacgg
     3721 taccgacagg ctataagaaa gacgatacag tctttaagtt ccaaatcgat agcgctggga
     3781 aactacttga tgcaaatggc aaagaaatca ccgctaactc aaaaccaacg acagatggtt
     3841 tctatttaac gagtgataat aagattgttt taatcaaata caatgaactg cgtgattttg
     3901 acttttcaat tcttaaaaag aactcgcaaa gcaacaaacc attagcagat actgaattca
     3961 gcttagccac aaaagaggat tccaatacga ttttggctac tctgaagacc aatgatgaag
     4021 gaacgggtca gtttttagat gcttcgggta atcattacgg tgttcgtcca ggaacttacg
     4081 ttatcaaaga aacgaaagca ccagaagggt ttgtcctttt agaagggatt ttcgaggtca
     4141 ctataaatgc ggacggaact gtcggcgacg tgacttatga tggtaaggat cttgataaag
     4201 atgctatcaa agtaaatcta aaagatgagg acaataacgt catcgaattg acggttgcta
     4261 atacacccaa aggacaactg ccagcaaccg gcgggtatac agactttcat catagtagcc
     4321 ttagctcttg tcacagcagc aggtgctctt acgatctgct acttctaccg taatcgaaag
     4381 gagttgaact aagatgaaga ataaatcatt gttcatcaaa atcaccttac ttctttcaac
     4441 tttgctggta gggatcttta cggggcaaca agcctttgca gatgatggga cgatcgatat
     4501 tgtgatccac aaacaattgt gggaccaagc acctgatgca actattcaaa atacgggagc
     4561 ggccgatcaa gataccaaag atccaatcga aggggtcggc tttacgattt atgacattac
     4621 agaggattac tataaattgg tcaaaggctc tgacgaaaca acaactattc aaatgattca
     4681 agaaaatgtt gctgattatc aaaaaattat cagagaacaa ggcctgactg atgaaaaagg
     4741 aacaatcaaa tttacccaac tacctaaaag cagtggcggt aaaaatgcgg tatacctgat
     4801 tgttgaaacg gcattgcctg atcctgaaaa ggcaaaagtc gaaaaagcgg ccgaaccctt
     4861 agtagtggct ttaccagtat atgaaatgaa cacagacggt aaaacctata cggacaaaga
     4921 attagctacc gttcaccttt atcctaaaaa tgtgggaaca atcgttgaaa agccagtgac
     4981 accaactaaa cctaaaaaac caagtaaacc gaagaaacgc ttgccacaaa caggtgaagc
     5041 aaaatccttc ctaggtatat tggggattct tttgattggt acggcgatca ttttatggcg
     5101 gaaacactct gtacgaaaca attaatcaaa ggtacaaaag actaaggtct attggaatat
     5161 tgtacaaaaa atcaaggagg aattagaaaa tgaagaaatt atggaaaaca ctttttgcag
     5221 cactacttgt aataccttta ttcgccggcg tgtttggtgc gaaaacagta agtgctgctg
     5281 aagcaacaac gacaaatgta acgatcaaca aacgaatctg gaaagatggc gaagctccag
     5341 cacaaggttc catgcaaaat actggggaag aaatggactt tggtggagat ccacttgaag
     5401 gagccggctt tacagcctat gatgttacag atgcttatct tgctctgatt gcagatggta
     5461 agtcacaagc agaagcaaca aaagaaattg ctgataatgc gtcagattac acaactgtag
     5521 caaaagcaga acaaaaaacg gatgccaatg gtcaaacaac ttttgaagga cttgctttaa
     5581 aagacggcga caaagataaa gtctatatgt ttgttgaaac aagcacgcca ggcaatgttt
     5641 ccgtaacgac aaaggccgct ccaatcgttt tagcaatgcc aatctatacg tttaaagatg
     5701 gcaaatattc agatacaatc aacaccaacg ttcaagtcta tccaaagaac gaaaccaaga
     5761 ctgataaaaa agaagttgcg aatttagata agttcactga agttaaggat gctgatggaa
     5821 atgttttata tcataacctt acaactggcg atgaaatcga atataaatta actttaaata
     5881 ttcctgcgga cattctggaa gatggcgtca cttactctgt aactgatacg ccaactgctg
     5941 gacttgctta tgtaaaagat tcttttgcag caactggttt agtagctgga actgactatg
     6001 aattagctga agattctgca actggcggat ttactgttac tttgaaacaa agtgaaaatg
     6061 taggaaaatt agctggtagt caattgattg ctacttacaa aatgaaatta actgctgaag
     6121 taaatcctga cgatttagta aacaatagtg cccaagtaac aattggtaac aaacctcaag
     6181 ataaaattac gcctccaaca caatttggta caggtggtta taaattcgtc aaaaaagatt
     6241 cacaatctgg tgcaacctta tctggtgcgg aatttgtcgt aaaacaagac tctaactttg
     6301 caatatttga agatgctaaa aacactaaag gtgaatatat ctttaaagaa tggacgactg
     6361 atgaagcaaa agcaacgaaa atcgtttctg acgacaaagg tgaattgaaa gtaattggtt
     6421 tgaaaaatgg tgactatcaa ttagaagaaa aagcgacttc aagtgataaa tatgtgttac
     6481 ttgatgaaga tgtcgatttc actgttgaac acggtcaata tggcagtcaa gaactaaagt
     6541 ctgttcttaa cacgcctaaa ggtctgcttc catcaacagg tggtaacggg atctatgcct
     6601 tcttgcttat cggtgctgct cttatgatcg gtgcttacgc ttggttcaag aaatctaaaa
     6661 cacaagctga agtctaagat atagttttaa aatagggaga aggcagcttc atttcttgaa
     6721 gttgcctttt ctttctttta aatagggaga aggaggtgca agcatggatc agcgccaaaa
     6781 aaacgaacgg atcaatttga ttttaaaact gctaatggcg attttattta cgaccggtgc
     6841 cattattttc agctatccct ttttggccaa tgcggtgaat tcctattatg accaaaagat
     6901 gatggaaaaa aacttggctg agatggccgc aacaaatacc aaagaacaag caaaaaggca
     6961 ccaagaaatg gccgaaaaaa ataaagagct agctgaaagt aagaacatga ccaatattcc
     7021 tggcatggga ctggtagaag acccgtttga aaatgaggtc gatgatgcca aagatccggg
     7081 taaagcctat tatgaagagc ataccgtggg tgctatctat attccaaaaa tttcggtcag
     7141 cttaccttta tttgatgaaa ccaatgctgc tcttttagaa aaaggcgcga cgattttaca
     7201 aggaacgtct tatccgatcg gtggcaatag tacccattcg gtgatctcag gccacagtgg
     7261 cttgacggaa cgtaaacttt ttacagattt agaaaagttg gtgattggcg atgattttta
     7321 tttgaccatt gctggggaaa aattggctta tgcggtcgat gacatccaga tcgttttacc
     7381 gtcggatatc gacaaagtgg tcatccaacc ggggcgggac ttggtgacct tattgacttg
     7441 tacaccttat ggcatcaata cccatcgttt attagtcact ggacaccgtg taccttatgt
     7501 ggaggaaatg gcggaggaag tgaccagtac caaaaaagcg gtcgaacggc gttttcgctt
     7561 gtatttacta ctgatcccgc ttttctttgc catgattttc tattggatgt atcgcaagtt
     7621 cgtctattat caaagtggca aacacagtta tgacttttgt ttttacttat tggaaaatgg
     7681 tcaacccaaa gcaggcgtga cttttacctt agtccgtaaa aaacgctggg caacagatgt
     7741 cactagccaa ccagttgctg taagccaggt ggatggatgg gtgtctttcc ctgagatcag
     7801 agggggtcgt tattatgcca aggcgatcga tgggtcgaca aaaccagtta agggcaaggt
     7861 ccgccgttta aaagatcggc agtttgtttt aagccgcgtc acgaagaaaa agcacggtaa
     7921 gaaagttact tattatctgg agaatggagc gaaaaaatga agaaaaacag gcaagtgatc
     7981 tcgcaaattg cgatcatcct catttttatg gtcggcatct tgaccatgct ctatcccttt
     8041 tatgtcgatg cgttgaataa tgtgatcgat caagtgcgcg tcgatcgcta tttgaaggaa
     8101 agccagaaag aatttgaagc tgaacgcaag cgactggcgg aagaaaataa taaattgagc
     8161 gacagcgggt tagctccggg tgccgatccg tttgcgaatc cgaatggtgg tacggtttca
     8221 gctgcttatt acaaaaagca tttgattggg accatcaatc ttcctgattt ggccattgag
     8281 ttaccgctat ttgatacgac caatgatgat ttattggaac aaggtgcgac ggtcctagat
     8341 ggcacgtcct ttcctgttgg tggcgcaagt acccatgcgg tgatctcggc tcatcgtggc
     8401 ttgccagaac gggaactctt caccaatctg ccggagctta aaaacggtga tatttttctg
     8461 ctaaatgtct taggggaaac cttagcttat gaagtgtttg acagccaagt cgtgacgcca
     8521 gatcagactt cggttttaaa gatcgaacca ggccaagact tggtgacgct catgacctgt
     8581 accccttata tgatcaatag tcaccgtttg ttagtcacag gaaaacgcgt gccatataca
     8641 ccggctgccg agaaaaaaca agtcaaaggc gatcgttttc gtaagttgaa acaaatcact
     8701 atcttggcgg ggacggctct gttgatctta gccgctattt atcaattgta tcatgtgatc
     8761 gctcgttacc gattgcggaa agtgcgtttt gatttcactg tttgcttaga aggcgtggca
     8821 gaacatacgc caatcgcact ctatgataaa aaagggaaaa aagccctgcg gcgtaatgga
     8881 aaagcctatc aagaactaac agatcaaacc ggacaagtca cttttaccga tctaccaggg
     8941 gattgctatc gtttgaaatt gggcaagagt tggttggtgc agtttggttt gaagaaaaaa
     9001 aagaggccat ccaagatttg gaagataaat aagaaaaaag ttatgttaaa agaagaaaga
     9061 atgctaaaag tcaaataaaa aatgctcatg ttatcaaatg atttaaa
//
LOCUS       DANKHK010000093         8980 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000093 DANKHK010000000
VERSION     DANKHK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(150..2252)
                     /gene="tcrB"
                     /locus_tag="PW109_002130"
     CDS             complement(150..2252)
                     /gene="tcrB"
                     /locus_tag="PW109_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase TcrB"
                     /protein_id="HDL2511931.1"
                     /translation="MSNNRKHSSHSHHNHGDMDHSKHDHNEMEHSQMDHSKMNHSAMD
                     HNEMDHGAMGGHAHHHHGSFKEIFLKSLPLGIAILLITPMMDIQLPFQIIFPYADVVA
                     AVLATILYIYGGKPFYMGAKDEFNSKAPGMMSLITLGITVSYAYSVYAVAARYVTGEH
                     VMDFFFEFATLILIMLLGHWIEMKALGEAGDAQKALAELVPKDAHVVLEDDSIETRPV
                     SELQIGDVIRVQAGENVPADGIIIRGESRVNEALVTGESKPIEKNPGDEVIGGSTNGG
                     GVLYVEIKQTGDKSFISQVQALISQAQGQSSRAENLAQKVAGWLFYIAVIVALIALVI
                     WMIIADVPTAVIFTVTTLVIACPHALGLAIPLVTARSTSLGASRGLLVKDRDALELTT
                     NADVMVLDKTGTLTTGEFKVLDVELFNDKYTKDEIVALLSGIEGGSSHPIAQSIISYA
                     EQQGIRPVSFDSIDVISGAGVEGQANGHRYQLISQKAYRRNLDMDIPKGATLSVLVEN
                     DEAIGAVALGDELKPTSKDLIQALKKNKIQPIMATGDNEKAAQGTAEILGIDYLANQS
                     PQDKYELVEKLKAEGKKVIMVGDGVNDAPSLALADVGIAVGAGTQVALDSADVILTQS
                     DPGDIESFIELAQKTTRKMKENLVWGAGYNFIAIPIAAGILAPIGITLSPAVGAVLMS
                     LSTVIVAINAMTLKLDPK"
     gene            complement(2391..2597)
                     /gene="tcrZ"
                     /locus_tag="PW109_002131"
     CDS             complement(2391..2597)
                     /gene="tcrZ"
                     /locus_tag="PW109_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone TcrZ"
                     /protein_id="HDL2511932.1"
                     /translation="MKKEVLLDGVKCAGCANTVQERFSAIEGVESVEVDLATKKAVLE
                     SQTEIDTETLNAALAETNYSVLSA"
     gene            complement(2683..5148)
                     /locus_tag="PW109_002132"
     CDS             complement(2683..5148)
                     /locus_tag="PW109_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2511933.1"
                     /translation="MENKSFAIEGMTCASCAQTVEKAAKKVRGVTQASVNLATEKLSI
                     EYDELTFSVENLQKAVDNSGYELIAQEGTTQTFAIEGMTCASCAQTIEKAVGKLSGVD
                     KASVNLATEKMQVSYNPSAISVSDVTGAVSNSGYAAVLETTETQDNSRAEKREKKEKR
                     MKQLSNRFGISIIFTIPLLIISMGPMVGMPLPNIVDPMINAFNFSLLQLILTLPIMVV
                     SWEYFQKGFKTLFKGHPNMDSLIALGTAAAFVYSLAATIGAGLGYGNFSDLLYYEVTG
                     VILTLHTLGLFLEERSKGQMSSAIEKLVNLVPKTARVIRNGVEQEITVDEVALGDVIR
                     VRPGESMPVDGVVVEGRTSVDESMLTGESIPVEKESGDEVIGASINKNGSIDYRATRV
                     GSDTTLSQIIKLVEDAQGSKAPIARMADIITRYFVPIVIALAVLAGIAWLIAGQSGIF
                     TLSVIITTLVIACPCALGLATPTSIMVGTGKGAEHGVLIKSGEALETTHNLDTIVFDK
                     TGTLTEGKPIVTDILVTPLITKENLLYYAASGETGSEHPLGEAIVQKSKEENMTLAKP
                     DHFEAIPGHGIRVEIEGKDMYIGNRKLMLEQKIDLSSMEKESDRLADEGKTPMYLSVD
                     GKLAGIIAVADTLKENSMKAVKELRRRGVEVIMITGDNKRTAKAIAKQVGIDSVLSEV
                     LPEDKAEEVKKLQEAGKKVAMVGDGINDAPALAQADIGIAVGSGTDVAIESADIVLMR
                     NDLTAVLTAIDLSHATLRNIKQNLFWAFAYNLVGIPVAMGLLHIFGGPLMNPMFAAVA
                     MSFSSVSVLLNALRLRRFKPAVV"
     gene            complement(5161..5616)
                     /gene="tcrY"
                     /locus_tag="PW109_002133"
     CDS             complement(5161..5616)
                     /gene="tcrY"
                     /locus_tag="PW109_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-responsive transcriptional repressor
                     TcrY"
                     /protein_id="HDL2511934.1"
                     /translation="MSTIAVDLHITDAEWEVMRVVWANGRVTSKEVISTLGEKMDWKQ
                     ATTKTLLGRLVEKGALNTEQEGRKYIYSANIEEKEAVRSFTNDIFDRICRKNVGNVVG
                     SIIEDHVLSFDDIQRLEEILEMKKAFAVEEVDCQCTEGQCECHLHHHGE"
     gene            complement(6178..7068)
                     /locus_tag="PW109_002134"
     CDS             complement(6178..7068)
                     /locus_tag="PW109_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDL2511935.1"
                     /translation="MNQEHFDTIKNKDGFIAALDQSGGSTPKALNAYGVSEDQYSNDD
                     EMFDLVHKMRTRIITSPSFTPDKIIGAILFEHTMNNRIEGQYAGDYLSDKGIVPFIKI
                     DQGLADEEHGVQLMKLIPGLNNLLKQAKEQNMFGTKMRSNILSYNEKGIDDAVTQQFQ
                     VAQEIIDAGLVPIVEPEVNIHSEDKEQIEEYLNQSIKNHLDALATDDYVMLKLTIPTK
                     ANLYQELADHSQVLRVVALSGGYETDEANEIPSQNDNVIASFSRALTQDLSVNQSDAD
                     FDAKLKEAVDSIYEASVVKN"
     gene            complement(7164..7844)
                     /locus_tag="PW109_002135"
                     /pseudo
     CDS             complement(7164..7844)
                     /locus_tag="PW109_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            7966..8931
                     /locus_tag="PW109_002136"
     CDS             7966..8931
                     /locus_tag="PW109_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2511936.1"
                     /translation="MDKTGTITVGKPQVTDIISIGRISENEILRIAAGLEDSSEHPLA
                     LAVINEAKDKKITPAVAKNFTAISGKGVQALIDGKQAFIGNDRLSDDFNMTDDLKVKM
                     TSLQAQAKTVVLVGYDGQIIALIGIQDAPKSSSKAAIRAMQKSGFHTVMLTGDNRLVA
                     QAIADDIGIDEVIADVMPGDKAQHIRKLQEKGAVAFVGDGINDAPALSTATVGIAMGS
                     GSDIAIESGGIVLVKNDLMDVVTSLVLARKTYSRILINLFWAFIYNVIGIPVAAGIFS
                     ALGFTLSPELAGLAMALSSITVVLSSLLLNYVRLPKNSETLIDNS"
BASE COUNT         2540 a         1921 c         1575 g         2944 t
ORIGIN      
        1 taaaaggact agcctcataa tttttcctcc ttataaacta aaaaaggaag agatgatttt
       61 ttaaatcatt tcatctcttc ctttcctgtg aaactattgt atttttacac gataaggtca
      121 ttcaattatt tcatgtgcca aataactgtt tattttggat ctaatttcaa tgtcatagca
      181 ttgatggcaa caattacagt cgaaagtgac atcaagacgg caccgactgc tggacttaat
      241 gttatgccaa ttggtgctag tattcctgca gctattggga tcgcgataaa gttataccct
      301 gctccccaaa caagattttc tttcatctta cgtgtggtct tctgagccaa ctcgataaag
      361 gattcaatat cgccagggtc tgactgtgtc aagatgacat cggctgaatc caatgctact
      421 tgagttccgg caccaacagc tataccaacg tctgctaatg caagagaagg tgcatcgttg
      481 accccgtcac cgaccatgat aactttcttt ccttctgctt taagtttttc aactaattca
      541 tatttatctt gaggagattg attagctaga taatcaatac ctaaaatttc tgcagttcct
      601 tgagccgctt tttcattgtc acccgttgcc ataatcggct gaatcttgtt ctttttaagg
      661 gcttgtatta agtctttact tgtcggtttt aattcatccc ctaaagctac agcaccgatt
      721 gcttcgtcgt tttctactaa gacactgagg gttgctcctt ttggaatatc catatccaga
      781 ttacgtctat atgctttttg actgattaat tgataacgat gtccgttagc ttgaccttct
      841 acaccagcgc cagaaataac atcaatcgaa tcaaaactta ctggacgtat cccttgctgt
      901 tctgcgtaac taataattga ctgagcgata gggtgactag atccaccttc gatacctgac
      961 aataaggcaa cgatttcatc tttcgtatat ttatcattaa agagttcaac atctaatact
     1021 ttaaactcac cagttgttaa agttccagtt ttatctaaaa ccataacatc tgcgtttgta
     1081 gtcaactcca aagcgtcccg gtctttcact aataatcccc ggctggcccc taagctggta
     1141 ctacgagcag tcaccaatgg aatagccaga cccagggcat gcggacaagc aataactaat
     1201 gtggtgactg taaaaataac tgccgttggt acatcagcta taatcatcca tatgacaagg
     1261 gcaataagcg ctacaataac cgcaatataa aagagccatc ctgcaacttt ttgtgcaaga
     1321 ttttctgctc gagaagattg gccttgagct tgactgatta atgcctgaac ctgagaaatg
     1381 aaggacttat cgcccgtctg ctttatttcc acatagagga ctccgccacc attggttgat
     1441 ccaccgatga cctcatctcc gggatttttt tcaattggtt tcgattcccc agtcacaagc
     1501 gcttcgttta cacgtgattc tccgcgaata atgataccat cagctggaac attttctcct
     1561 gcttgaacac ggataacatc tccaatctga agttcagaca caggacgagt ctcaatcgaa
     1621 tcgtcttcta aaacaacatg agcatctttc ggcaccaact cagccaatgc tttttgcgcg
     1681 tcccctgctt cacccaatgc cttcatttca atccagtgtc ctaataacat gattaaaatt
     1741 aacgttgcaa actcaaagaa gaagtccatg acatgttctc cggtcacata tcgagcggcc
     1801 actgcgtaaa cactataggc ataagaaacc gttattccca aagtaatcaa ggacatcatg
     1861 cctggagctt ttgaattaaa ctcatctttc gcacccatgt agaatggttt tccgccataa
     1921 atgtatagaa ttgttgccaa tacagctgct acaacgtctg cataaggaaa aataatttgg
     1981 aaaggcaact gaatatccat cataggagta atgagtaaga ttgcaattcc taatgggagt
     2041 gacttcaaga aaatctcctt aaagctaccg tgatggtggt gggcatgccc tcccattgcg
     2101 ccatgatcca tttcattgtg atccatcgca ctatgattca tcttgctgtg atccatctga
     2161 ctatgttcca tttcattatg gtcgtgtttt gagtgatcca tgtcgccgtg attatgatga
     2221 ctatgtgacg aatgttttct gttattactc atttttaatt cctccttatt atattagaga
     2281 ataattttaa agttttagaa ctttctcatc taacactgca tataattctc atgaaaaggg
     2341 ttctatactt tatctctatc tactatctat gaataaatgg tattcgtact ttatgcactt
     2401 aatactgaat agttagtttc tgctaaagca gcattgagcg tttcggtatc aatctctgtt
     2461 tgactttcaa gtactgcttt tttagtcgct aaatcaacct ctacagattc aaccccttca
     2521 atagctgaaa atctttcttg taccgtgttc gcacaacctg cacattttac gccatctaat
     2581 aagacttctt ttttcattat tctattcccc tttttatttt tttatcttct cctattatat
     2641 agaataggaa gaagtttaaa tcccaaataa gctacgtgtt ttctaaacaa ctgctggttt
     2701 gaatcgtctt aaccgtaaag cattgagcaa gaccgatacg gagctgaaac tcatggcgac
     2761 tgccgcaaac attggattca tcaatggtcc gccaaaaata tgcaagagac ccatcgcaac
     2821 tggaatacct acaaggttgt aagcaaaagc ccagaacaag ttttgtttaa tgtttcgtag
     2881 cgttgcatga cttaaatcaa tcgcagtcaa tacagcggtt aaatcattac gcataaggac
     2941 aatatcagcc gattcaatcg ccacgtctgt acctgatcca actgcaatac cgatatctgc
     3001 ttgagctaat gcgggtgcat cgttaatgcc gtctccaacc atcgctacct tcttgcctgc
     3061 ctcttgtagt tttttgactt cctctgcttt atcttcaggt aatacttcac ttaatacact
     3121 gtctataccc acttgcttag caatcgcttt ggctgtacgt ttgttatctc cagtaatcat
     3181 gattacttca acaccgcgtc ttctaagttc tttaacagcc ttcatactat tttccttgag
     3241 tgtgtcagct actgcgataa ttccagctag ttttccatca acagagagat acatcggtgt
     3301 ttttccttcg tctgctaaac gatcggattc tttttccatg cttgataaat caatcttttg
     3361 ctctagcatc agtttacggt ttccaatgta catatctttg ccctcaattt caactcgaat
     3421 cccgtgtcca ggaatcgctt caaaatgatc tggtttagct aatgtcatgt tctcttcttt
     3481 tgatttctgt acgatggctt cgcctaatgg gtgttcagaa cctgtttcac cggaagctgc
     3541 ataatataaa agattttctt tagtaataag aggagttacc aaaatatccg tcacgatagg
     3601 tttaccttct gtcagtgtcc ctgttttatc aaatacaatc gtgtctaaat tatgagtggt
     3661 ttctaacgcc tcaccacttt ttatcaagac accatgttct gccccttttc cggttccaac
     3721 cataatactt gttggagtcg ccaaacctaa ggcacatgga caagcaatga caagggttgt
     3781 tatgataacg gataaagtaa atattccaga ttgacctgca attaaccaag caatccctgc
     3841 taaaacggct aatgctataa caatcggtac gaaatatctg gtaatgatat cggccattcg
     3901 agcaatgggt gctttagacc cttgagcatc ttctactaat tttatgattt gagatagagt
     3961 tgtatcactt cccactcgag tcgcgcggta atcaatggaa ccatttttat tgatactagc
     4021 cccaataacc tcgtctccac tttccttttc cacggggata ctttctcctg tcagcattga
     4081 ttcatcgact gaagtgcgtc cctcgacaac aacaccatct acaggcatac tttctcccgg
     4141 acgaactcga ataacatctc ctaaagcgac ttcgtccaca gtaatctctt gttccacacc
     4201 attacgtatc actcgagctg ttttaggaac caagttgact aatttttcaa tcgctgagga
     4261 catttgaccc tttgatcgct cttctaaaaa taaccctaac gtatgaagag ttaagataac
     4321 tccagtcact tcatagtaaa gtaaatctga aaagttcccg taacctaagc cagccccgat
     4381 cgtcgctgcc agactgtata caaaagcggc cgcggtacca agggcaatca gggaatccat
     4441 atttgggtga cctttaaata aggttttgaa tcccttctgg aaatattccc agcttactac
     4501 cattatcggc aaagtcagaa taagttgtaa aagagaaaaa ttaaatgcat ttatcattgg
     4561 gtcaacgata tttggtagag gcataccgac cattggaccc atggatataa ttagtaaagg
     4621 gattgtaaat ataatggata tcccaaaacg atttgaaagt tgcttcattc ttttttcttt
     4681 tttctcacgc ttctctgctc gtgaattatc ttgagtctca gttgtttcta atacggctgc
     4741 ataaccacta ttggatacag caccagtcac atcagatact gaaattgctg acgggttata
     4801 agaaacttgc attttttctg tcgctaaatt gactgaagct ttatctacgc ccgacaactt
     4861 tccgacagct ttttcaattg tctgtgcaca ggatgcacag gtcatccctt caattgcaaa
     4921 ggtttgcgtc gttccttctt gggcaatcag ttcataccct gaattatcca ccgctttttg
     4981 tagattttct accgaaaaag ttagttcatc gtactctata cttaacttct ctgttgctag
     5041 gttcacggaa gcctgtgtga ccccacgtac tttttttgcc gctttttcta ctgtctgcgc
     5101 acaagacgca caggtcatac cttctatggc aaacgattta ttctccaatt ttctcgctcc
     5161 ttattctcca tgatgatgta aatggcactc gcattgccct tctgtacact gacaatccac
     5221 ttcttctact gcgaaagctt ttttcatctc taatatttct tctagtcgct ggatatcatc
     5281 gaagcttaag acatgatctt caatgatact ccctactaca ttcccgacat tctttcgaca
     5341 aatacgatcg aaaatatcgt ttgtaaaact tcttaccgct tctttctctt cgatatttgc
     5401 cgaataaata tattttctac cttcttgttc tgtattcagt gcgccttttt ccacgagtcg
     5461 acctaagagc gttttggttg tcgcttgttt ccagtccatt ttctctccca atgtagaaat
     5521 gacttcttta ctagtcactc gaccattcgc ccagactaca cgcataactt cccattcagc
     5581 atcggttata tgaaggtcta cagctatagt actcatattc tctccccctt tcgtttacgc
     5641 gcgtaagcat taatgctaaa tagagtttac actagtaaac gattattgtc aagagtttat
     5701 gtaaatgaat aatttgtgat ttattatttt ttataacttt ttaactttcg tttcgatgcg
     5761 acacgcctga cgaaaatatg gattatgttt tagttcatta attagggatt ggttcgtcgg
     5821 acacttctct gttaaaaaaa caccgaaaat aagctttttc agaatgtcgg cggatagaca
     5881 tcccactgta cgggttttct atcccccgat ttcattttga ttacaaattt gagcctaaca
     5941 ggctcaaggg gtcacagcat cgcaaattat tcatctacaa actatttcag ctttaaacac
     6001 aaaaaaagca aggtgagagc cctgcctttt tgcgtcttta tcggactata attgtatgag
     6061 tagctcattg ggaagtacca tcttaaaatc taggagaatg atatactact cgctgtctat
     6121 actataactc gaatgtaagt tctggtatat gaatattgga agtttttttg caaagattta
     6181 gttttttaca acagaagctt cataaataga atccactgct tcttttagtt ttgcatcaaa
     6241 gtctgcatca ctttgattaa cagataagtc ctgcgtaagc gcccgtgaaa aacttgctat
     6301 aacattgtca ttttgactag gtatttcgtt cgcttcgtct gtttcataac cacctgataa
     6361 agcaacaaca cgtaaaactt gtgaatgatc ggctaattct tgatataaat tagctttcgt
     6421 tgggatagtt aatttcaaca tcacataatc atcagtagct aatgcatcta aatggttttt
     6481 aatggattgg tttaaatatt cctcaatttg ttctttatct tcagaatgaa tattcacttc
     6541 aggttcaacg ataggaacta agccagcatc aattatttct tgagcaactt gaaactgctg
     6601 ggtgactgca tcatcgatgc ctttttcatt atatgaaaga atgtttgaac gcatttttgt
     6661 accaaacata ttttgttctt ttgcttgttt aagtaaatta ttaagtcctg gaattagctt
     6721 cattaattgt acgccatgtt cttcatctgc aagtccttga tctattttaa taaacggaac
     6781 aattccttta tcgcttaaat aatctccagc atactgacct tctatcctat tattcatcgt
     6841 atgttcaaat aaaatcgcgc caataatttt atccggagta aaagatggag atgtgatgat
     6901 tcgtgtacgc attttgtgaa caagatcaaa catttcatcg tcatttgaat actggtcttc
     6961 actgacccca taagcattca gagcttttgg tgtactacca ccgctttgat caagtgccgc
     7021 gataaagccg tctttattct ttatagtatc aaaatgttct tgattcattg ataaattccc
     7081 ctccatagat tttctgaaat tcccatagtg gttctgttgc gaagtttgaa aatcaaacgc
     7141 gccctctctg aactccaaat atcttaggct gttattccca ttaatacctt gatttcagta
     7201 gacaccgaaa agccgaagag cgttccattt cttcggttct ttttatatat tcctcgaatg
     7261 gtctccatgc ccttaatcgt ggaagaggct gtacggagac tttgataaaa tttatttcgt
     7321 cgtttaatag gtcgatggtc ttgttctatt aaattgttaa gatacttcac agttcggtgc
     7381 tctgtcttag tatataaacc cacactctgt aactttctaa aggtggagcc aagagaaggt
     7441 gctttatcgg tcacaattgc tttcggctca ccaaactgtt tatggagtcg ttttaagaaa
     7501 gcataggctg cttgcgtatc ccgtttcttt cgtaaccaga tatctaaggt taagccgtcc
     7561 gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata ggtttcgtcc
     7621 attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata gaggactttg
     7681 ctgtactctt gtacccaacg ataaatcgta gtatgacaaa catttattcc acgatcatat
     7741 aacaattcct gaacttaacg atagcttaga ttgtaacgca ggtagtaacc aacagcgaca
     7801 ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct gtcctctctg
     7861 tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa cagaaccaaa
     7921 aatgctgagg ttcttgaagc gacccacgat ataaaaaccg tcgtcatgga taagactgga
     7981 acaattactg ttggcaaacc acaagtgacc gatattatct ctatcggaag aattagtgag
     8041 aacgagattc tgcgaatcgc tgctggactt gaggattcat cagaacaccc attagcttta
     8101 gcagtaatta atgaagcaaa ggacaagaaa attactcctg ccgtagctaa aaatttcact
     8161 gctatttctg gtaaaggggt acaagctttg attgatggta agcaggcttt tattggtaat
     8221 gatcgtttat ccgatgactt taacatgaca gatgatctta aggttaaaat gacatcttta
     8281 caggcgcagg cgaaaactgt ggtattagtt ggttacgatg gtcaaataat tgctttaatt
     8341 gggattcaag atgcacctaa gtccagctct aaagctgcta tcagggcaat gcaaaaatca
     8401 ggatttcaca ctgtaatgtt aactggggac aatcgtttgg tcgcacaagc catagcagat
     8461 gatattggga ttgacgaggt catagcagat gttatgcctg gggacaaagc acaacatatt
     8521 agaaagttgc aagaaaaagg agcagtcgcc tttgtaggtg atggaatcaa tgatgcccct
     8581 gcattatcca cggcaacagt aggtattgct atgggatcgg ggagtgatat tgcaattgaa
     8641 tctggaggta ttgtactagt caaaaatgat ttgatggatg ttgtaacttc tttagtatta
     8701 gcacgaaaaa catatagtcg gattttgatt aacttatttt gggcttttat atataacgtg
     8761 ataggtattc cagtcgcagc gggtatattc tcagcacttg gatttacact atctccagag
     8821 ttagctggtc ttgcaatggc acttagctca attactgttg ttttgagctc actattattg
     8881 aactatgtgc gcttgccaaa aaatagtgag acacttatag ataatagtta gtatttgtaa
     8941 tatacaaaag tgacgctttt aattataata gtaattttcc
//
LOCUS       DANKHK010000094         8850 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000094 DANKHK010000000
VERSION     DANKHK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(315..398)
                     /locus_tag="PW109_002137"
     tRNA            complement(315..398)
                     /locus_tag="PW109_002137"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="PW109_002138"
     CDS             926..1426
                     /locus_tag="PW109_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2511937.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="PW109_002139"
     CDS             complement(1616..1960)
                     /locus_tag="PW109_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2511938.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="PW109_002140"
     CDS             complement(2114..3034)
                     /locus_tag="PW109_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2511939.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="PW109_002141"
     CDS             3221..3712
                     /locus_tag="PW109_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2511940.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="PW109_002142"
     CDS             complement(3736..6111)
                     /locus_tag="PW109_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2511941.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="PW109_002143"
     CDS             6437..7063
                     /locus_tag="PW109_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2511942.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="PW109_002144"
     CDS             7086..7790
                     /locus_tag="PW109_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2511943.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="PW109_002145"
     CDS             7982..8815
                     /locus_tag="PW109_002145"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2511944.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2683 a         1682 c         1562 g         2923 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt
//
LOCUS       DANKHK010000095         8758 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000095 DANKHK010000000
VERSION     DANKHK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..405
                     /locus_tag="PW109_002146"
     CDS             <1..405
                     /locus_tag="PW109_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511945.1"
                     /translation="AEKFSLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMI
                     LTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEY
                     WTYFIGILEGIIFYIGYFPKKNNNIYFYRNKL"
     gene            complement(531..1208)
                     /gene="nth"
                     /locus_tag="PW109_002147"
     CDS             complement(531..1208)
                     /gene="nth"
                     /locus_tag="PW109_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2511946.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKT
                     LKKKPRL"
     gene            complement(1215..1913)
                     /locus_tag="PW109_002148"
     CDS             complement(1215..1913)
                     /locus_tag="PW109_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2511947.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2181..3281
                     /locus_tag="PW109_002149"
     CDS             2181..3281
                     /locus_tag="PW109_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2511948.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRRPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLFTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFITLSG
                     ILLLPILFLFGIICYGFGDSIFTPVFNGQLSNSVSDNQQGLVMGGTQSIQSLSRVIGP
                     LIAGQLYAAAPFLPTVFGFLLMIFAYYHYNKK"
     gene            complement(3287..3730)
                     /locus_tag="PW109_002150"
     CDS             complement(3287..3730)
                     /locus_tag="PW109_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2511949.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3956..4243)
                     /locus_tag="PW109_002151"
     CDS             complement(3956..4243)
                     /locus_tag="PW109_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511950.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4414..5766)
                     /locus_tag="PW109_002152"
     CDS             complement(4414..5766)
                     /locus_tag="PW109_002152"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2511951.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6039..6485)
                     /locus_tag="PW109_002153"
     CDS             complement(6039..6485)
                     /locus_tag="PW109_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2511952.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6721..7647)
                     /gene="cysK"
                     /locus_tag="PW109_002154"
     CDS             complement(6721..7647)
                     /gene="cysK"
                     /locus_tag="PW109_002154"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2511953.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7787..8674)
                     /locus_tag="PW109_002155"
     CDS             complement(7787..8674)
                     /locus_tag="PW109_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2511954.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2385 a         1780 c         1441 g         3152 t
ORIGIN      
        1 gctgaaaaat ttagtttatt attaaataaa tttactttaa ttgctcctat ccttgcattt
       61 attatttttt ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct
      121 catgcaatga ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg
      181 agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta
      241 tcacttatca tttttattac acctttagat agatacgtca cttatttata taatcctttg
      301 gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt
      361 cccaagaaaa ataacaacat ttatttttat cgcaataaat tgtaataagt taccatccta
      421 gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc
      481 catgataaca agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt
      541 ggtttctttt ttagcgtctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt
      601 gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa
      661 aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt
      721 tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt
      781 tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt
      841 gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc
      901 ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga
      961 tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca
     1021 tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc
     1081 gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct
     1141 ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg
     1201 cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct
     1261 tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct
     1321 tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca
     1381 acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag tttcaaaatt
     1441 tccggctgat aatggtcttc ttctagccat tgaccgattc gctggaactc gatcgaagaa
     1501 agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt
     1561 gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg
     1621 ggataaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata
     1681 aacccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgctt gattcccata
     1741 tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac
     1801 tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatggtaatt tttaagtaaa
     1861 agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct
     1921 cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc
     1981 tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat
     2041 gtttttgatg cgcatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct
     2101 tagaatgtta acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta
     2161 ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat
     2221 acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt
     2281 taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt
     2341 ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt
     2401 ttgggcgtcg tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt
     2461 ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg
     2521 caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa
     2581 ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta
     2641 tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta
     2701 ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac
     2761 gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca
     2821 agatttcatc cgttattcct ttattcacag caggttttgc tgtttggttg gcagctggct
     2881 ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg
     2941 ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg ttcattacat
     3001 tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt tatggtttcg
     3061 gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc tcagataacc
     3121 agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc
     3181 cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta ttcggatttt
     3241 tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat ttgctacttc
     3301 gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct tcttttgaca
     3361 tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc tcgtgtctat
     3421 tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc
     3481 tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta ctgatggctc
     3541 ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg attttcccga
     3601 tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt tccatttctg
     3661 gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt agcagttgtc
     3721 ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt ttcctattca
     3781 acaaagataa aaatattttg taggaaaacc caatctgaaa ccaagaaaaa aaggggttga
     3841 caaagcttgt cataacccct ttttctgatt aaaagatagt aaagagacag tcatcggtaa
     3901 ttgtgatgat tttttgtcat cacttaagga tgagcggcct tttcaaaaac tgtctttaaa
     3961 gatcatcatc agtttctagc acatatggct gaccttcata tgtgtactct gtattcaatg
     4021 tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg aaaacgatga gaaacattat
     4081 ctttggcgtc atttgctggc atcgtgtaat attcctcttg cgtttcattc aactgagaga
     4141 gtacttgatc tgccaactca tcaatattga tcgttaattt cttcgaagca cttttataat
     4201 cttctaaaaa tgcatcttgt tggacaaaat attctttatt catcttcttt ctcacctttc
     4261 ttttcaaaat caaataaagc tgcttgttct tttatgtaac agctttatct ttgctgctta
     4321 ttgtgacaaa aaattcgaag aaagacaact aattgtactt ttcacaaaaa aagacagagg
     4381 gtcgtgacaa aattgtgtca caaccctttt tctttattgt gcagtttcga tttcgcctag
     4441 cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg tcaaaatgtg gttggaagaa
     4501 gaaatctgaa agtgcgagat cttctacagt cattttattt tgaacagcta aagacatggt
     4561 atttgctgat tgtgtaatgt cgtatttgga catgaactga gcacctacga tacgattcgt
     4621 tcctttttca tagactagct ccatcattac tttttcagta gttggcatga actctggacg
     4681 ataattatct tcaaatacag tcgcttggac ttctagatcg ttcaaagttg cgctttcttt
     4741 ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa agtcctgaag ttccttgagt
     4801 accacgatac gccattttat gatctgtcaa gttatttccc acaagcaaac cttgacgaac
     4861 ggcatttgtt gccaaaggaa tatagttttg cgtgccactt ggattgtagt tgactactgc
     4921 agagtctcca gctgcaaaaa tgtctggatt acttgtctgc atgtattcat tcactttgat
     4981 tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt tttgtatttg gacggaagcc
     5041 cacacataaa atgaccatat ctgtatcaaa tgtatctgtt ggtgtagcta cttgtttcac
     5101 ttttccttct tcatctgtga taaattcagt aacattttcg cctagcgcaa gagtaacacc
     5161 gcggtctgtc aattcttttt ctaagatatc tgtgaatggt ttatctagat atttattcaa
     5221 gatgcgatct aatccatcaa tcaatgtcac ttgttttcca gattcgacaa aagcttctac
     5281 taattcaata ccgatatatc caccaccaac aattactaca cgttttgctt ctttcgcttg
     5341 tgcaataatt tcatttgctt gattgtagtt tttgcatagg agtacatttt tcgcatcgat
     5401 tccactaatc ggtgggataa taggccatga accagttgtc atcactaatt tatcataaga
     5461 aaccgtttct gtttctcctg ttttcagatc ttttgccgtt actgttttat tttctgtatc
     5521 gacgtctgtt acatcgtgtt ccattttaac tgttgctcct aatgaagata attcttcagg
     5581 atttgagtag aacaagcctg ctgggtcctt tactactccg ccgacataaa gtgcgatacc
     5641 gcaagataag aaagaaatat tgtcatttct ttcatatact gtcacttctg cttctggatg
     5701 attttttaaa atacttttta cagctgcagt acctgcatga gtacagccaa ttactactac
     5761 tttcatttat ggtccctccg attaacattt tgtccttcga caaaactact ataacagaaa
     5821 taaaacggat acaacagttt aatgcttgtg aattcacaaa ggtttgtttg tagtttcatt
     5881 ccttttcaag cataagaacc caatatctac aagattctta ttttaataat attcactttt
     5941 atatctaaga ttgtgaataa aagacaaaaa aataaaaaag ctagcgtttt ttttacacag
     6001 ctagcttttt ttaggtataa cacttgtaga aactgttttt acatttgcgc cttttttgct
     6061 gactctttac aatccggaca gagtccatac aattccaacc gatgttcatt aatctcaaaa
     6121 tcagtcagtc tgcttgcagc catttctata tcttcaagcc ctgggtaatg aaaatcaaca
     6181 atcttaccgc agttttcaca aattacatga taatgacgct ttgaactgaa atcaaatcgg
     6241 ctagaagcat cgccgtactt catttcttga acaaatccaa tcgaagtaaa taagcgaagg
     6301 ttattgtaaa cagtcgcaac actcatattt ggaaaccgat gttcaagtgc tttataaatc
     6361 tcatcagccg ttggatggga atgatgttca atcaaatatt ctaaaaccgc gtaccgttga
     6421 ggtgtgattc gaatgttctc tttttttaac ttttcgataa cctcttcgac taacttattt
     6481 tccatgtaaa caccccacgt tgttattctc gtcttatcat actattttta cattttaaaa
     6541 tccacttaac atgaaaagaa aattcaattt tattgtattt taatgaaaaa agacgtaaaa
     6601 cagtcgcgtt cactattcta cgtctctatt caaataattt ttctcatcat aaatttttgt
     6661 gtgttctttt catctttaaa agttttttac ataaacctat ctggagaaaa accaagtgcc
     6721 ttatatctct tggtaaaggg ctgtagataa ataacgttca ccattgtcgg gaaccacagc
     6781 taataccttc ttgccttttc ctaattcttt tgcgactttt agcgcggcag caattgcagc
     6841 gccagatgag atgcctacta aaatcccttc ttttcgccca acttctctag ctgtttccat
     6901 cgcttcgtct ccactaatag aaaggacttt atcatacaca gaagtatcca gtgttttagg
     6961 aacaaaacct gtaccgattc cttggatttt gtgaggtccc gcttctttcc cttctaaaat
     7021 agcagattct gctggttcta ccccgattaa ttcgattttc ggatacacac gttttaattc
     7081 tcggccagcg ccagtgatcg ttcctcctgt accgatacct gcaacaaacg cgtccaagcc
     7141 atttactcca aatgcttgat gtatctcagg accagtcgtc ttctcatgca caagaggatt
     7201 tgcttcattt tcaaattgta atggtaaaaa gtagccgttt tcttttgcca aacgttctgc
     7261 ttctctgatt gatccagaaa taccatccgc accaggagtc aaaatcaact ctgcgccgta
     7321 ccctttcatc aataaacgtc tttcgatact catcgtctca ggcatcacga tgattacttt
     7381 atatcctttt gcaactccta ccatcgacaa tccgattcct gtatttccag aagtaggttc
     7441 aatgatagta tcacctggtt tcagtagacc atcgtgttct gctttttcta tcatacttaa
     7501 agcaatacga tcttttacac ttcctcctgg attaaaaaac tcaagcttta caaaaacatc
     7561 tgctgaatct tctggtacga ttttatttaa tttgacgatt ggcgttttgc caatcaattc
     7621 ggtgactgag ttatagatag ctgtcatctt caattcctcc ttgtctttgc tcttcttact
     7681 tatcataata caatctgttc atttatggcg agtgattcgt ctttgactag caaaaaaaag
     7741 acaaagatat accatatatg gtaatttctt tgccttttta tctaaatcaa acgtttagtt
     7801 cttgaccaac taataaagaa taactggaca tagacatgcc atttttcgcc attagatcat
     7861 caacagacat gccaaatttt tgtgcaatat cccatagcgt atcacctgac acgactgtat
     7921 atgtctgacc attatttgta tttacatctg ttgttgaaga tgatgacgtt gaagtcgtcg
     7981 tagtagtcgt tgttgttgta cttgtggttg ttgtagccga acctgttgat ggcgtatcaa
     8041 aacgggttaa attataagta ttaatcaatg agttcaattt ggccccataa tttggatcgg
     8101 tagcataacg acctgtcaag tacgctgttg catcataaaa acttgttgta ttgcttttcc
     8161 acacgccagc ataatgataa tcgcccgtgg aaaaactggt tgaacgaaga acattcgcat
     8221 ggtcttggaa ggattcccaa taagaagaat attttcggaa tggctcattc atcgttaccc
     8281 attgtccgtt tacatattct ttggttgcca tataaacgac ctgtccgtta tagcttcctt
     8341 tcacaccgaa tagattataa tttggcgcac tagctaatcc ggaagtgcca tatgcacttt
     8401 ccaacaacgc ttgagcgatc atgactgaag catacaagtc attacttgca gcaacttctg
     8461 acgcagacca gccgatttga tcgataaacg cttgaggatc agcgactgtt tgagatcctt
     8521 gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat catcggccca actaaaagac
     8581 tcgttccaat taaaggagca atcgcttttt tattgatcac catttttctt ttaggtctat
     8641 ggtgccgggc actgcgggtc ttcagctctt gcattcaaat tcctcctcaa aataaaaaaa
     8701 cttaataaaa agttctcttc ttaaaacaaa cttaattaat taaattatac ttttaatt
//
LOCUS       DANKHK010000096         8603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000096 DANKHK010000000
VERSION     DANKHK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            87..566
                     /locus_tag="PW109_002156"
     CDS             87..566
                     /locus_tag="PW109_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2511955.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            783..1874
                     /gene="phnW"
                     /locus_tag="PW109_002157"
     CDS             783..1874
                     /gene="phnW"
                     /locus_tag="PW109_002157"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2511956.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1867..2652
                     /locus_tag="PW109_002158"
     CDS             1867..2652
                     /locus_tag="PW109_002158"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2511957.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2998..4689)
                     /locus_tag="PW109_002159"
     CDS             complement(2998..4689)
                     /locus_tag="PW109_002159"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2511958.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5111..6058)
                     /gene="arcC"
                     /locus_tag="PW109_002160"
     CDS             complement(5111..6058)
                     /gene="arcC"
                     /locus_tag="PW109_002160"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2511959.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6173..7192)
                     /gene="argF"
                     /locus_tag="PW109_002161"
     CDS             complement(6173..7192)
                     /gene="argF"
                     /locus_tag="PW109_002161"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2511960.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7283..8512)
                     /gene="arcA"
                     /locus_tag="PW109_002162"
     CDS             complement(7283..8512)
                     /gene="arcA"
                     /locus_tag="PW109_002162"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2511961.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2473 a         1749 c         1585 g         2796 t
ORIGIN      
        1 tttaggtata aaagacgaat attattgggc aatccttctt ttttttgtta tgatacatgt
       61 gaaatctttc gtaaaggaga aagaaaatgc gcaaagctca aagacatttg ctgattaaac
      121 aagtcattga agaattcccg atccgtacgc aagaagaact attaaacaaa cttgggcatt
      181 atggcgttac cgccacgcag gcaacgattt ctcgagatat ccgtgatcta aaaatcgtga
      241 aggcacctga tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa ggaactcctg
      301 agactaggac tgaggaagag gatcggctgg ttcaaatgat tgaagacatt gtattgaaag
      361 tcgaacgagt ccactttttg acgatcgtca ataccttgcc agacaatgct catttatttg
      421 catctgtttt agatgagatc aaacctccct ctattgtcag tacaattgct ggattcgata
      481 cgatcatggt catctcggaa aatgaggagg acgcaaaacg agtagaagaa tttcttcacc
      541 atccaactga agtatcactt ttttaatcag agcacgtatt cctaatatgg aatgcgtgtt
      601 tttttatttt caatattttc cttattaaat aaacctttac actctttaca aaaatacatc
      661 attttttatt caattttttc aacaccttga ttgaatcaca ataatcatca tttatattgg
      721 atgttaacaa gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg aaaggaaatc
      781 atatgtataa attattaact cctggaccat tgacgacttc tgaaacagtg aaaaatgcta
      841 tgctgatcga tcattgtact tgggatcaag aatacaaaga aattacacaa tggataagaa
      901 aaaaattatt ggatcttgct caagtatcgg aaggaatata cacaacggtc ttaatgcaag
      961 gtagcggctc ctttggggta gaatcggtat taagttctgt gatcaggcca gaagatactg
     1021 tactgatttg ttcaaacggc gcatatggac aacgaatgat caacatgtgc gaatgtctga
     1081 aactgaacta taccgtttat gaggtatcgg aacatcaaat cctccaagct catgtgatta
     1141 cacagctgat tgaagagaat ccctctatct ccgcactagc aatggtccat agcgagacta
     1201 cttccggtat tttgaataat ttggaagaga ttgctgctgt cacacaagaa aaagatcttt
     1261 tgtttatagt ggacgcgatg tcaagctttg gtgggatacc gattcgtgta gccgaactag
     1321 ggattgattt tttgatttcc agctcaaaca aatgtattca aggtgttcca ggcttttctt
     1381 ttgtacttgc taataaagcg gaactagaaa agcgaaaaaa tgtgagtcga agtttgtcac
     1441 ttgatctata tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg cgtttcactt
     1501 cccctaccca tacggttttg gcttttcaac aagcgctgaa ggaactagag gcagaaggag
     1561 gaatcgaaac tcgatattca cgttatctcg ccaataatca tgtactacga caaaaaatga
     1621 cagagatggg gttccagcct ttcattcaag aatatcaggg gccattcatc acctcttttc
     1681 tttaccctaa ggactggcga tttgatttta atcattttta tcagtattta aagcaaaacg
     1741 gatttgtgat ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc ggaaatattg
     1801 gcgagatcta tccggaagat attttggaac tgacagcaat tatcaaagac tataaggaga
     1861 acgttaatga ttaaaactgt aatttttgat tgggcaggaa caactgtgga ctttggctgt
     1921 atggctcctg tccacgcttt tagaaatgct tttctagaaa aaggaatcca actgactgac
     1981 aaagaaatcc gcgaaccgat gggaaaatta aaatgggatc atatccaaca gcttctttcc
     2041 ctcccttctg tcaaaaaaca atggatagct atttatggac aactacctca ggaaaaagag
     2101 gtggaggaat tgtatcaaca atttgaacat tatctgttta aagatttggt caagcattct
     2161 accttgaaac ccgacacatt ggaaacgatc gaacggttga gaaacgctgg gatcaatatt
     2221 ggttccacga ctggatttac tgctcctatg atgacaatcg tagcagaaac cgcaaaaaaa
     2281 gaagggtatt caccagactt catggcaact tctgaagacg tcgaagggta tggtcgaccg
     2341 tttccttata tgatccataa aaacatgcag tacttcaaaa acaagtcggt tgatgaagta
     2401 atcaaagtcg gagatacggt ctcagatatc caggaaggga aaaatgccgg agtgttgaca
     2461 gtaggtgtaa ttgaaggtag ttcgctaatg gggatttctt atgatgagta ccaaaaatta
     2521 gagatgggga aaaaagatcg tttaaaacag tctgttgctc ttagattcca acaagctggc
     2581 gctgatattt gtgtaaacaa tttaaaagag ttaacagaat acattttgta cgctgacaag
     2641 aatccagaat gacttttata caaaatcaga ggtcgtgaaa aagctgtact gcatcaagta
     2701 ataagaacag ccaaacaaaa atagctcctc atatttttcg tttggttagg cttattaccg
     2761 cagatgcaag cttttgaaca ccgtttattc gaaaaaaaaa gggagctgtg acacgacttt
     2821 tgtcacagtc cccttattta caaacaaaca gtgtttaaga gacaacctct ccactaaacg
     2881 gtgttcattc agctgactct cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt
     2941 ttctcttttg tttctctaat actcggatca gaacgctgat ttcacattct ctttttctta
     3001 catcttatct ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata atacagtcac
     3061 tgcataaact agagctaagc gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg
     3121 agcatagtat ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg
     3181 ttcgtatttt tccccagcag acaagaccgt ttcagggtat ttttgaacta atttaaccac
     3241 ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga caaatcctgc
     3301 tttttccaac aggctgactg cacgagcatg tgtatattgc acataaggtc cagtttctcc
     3361 ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa
     3421 atcgtggaaa atgactgccc caaccccaac ttgtttagct acagcatctt tgttttccaa
     3481 atcagggttt ttctcgctga tttgttcttt agcagattgg attgcttcat ttaatacttc
     3541 ttctaataag acgatctttc ctttacgagt tgataatttt ttaccacctt gcgtgatcaa
     3601 accaaatgga atatggcgca tatcgtcaga ccagtcaaag cccatttcct tcaagacagc
     3661 tttcaactgt ttaaaatgat agctttgttc attccctact acatatagag attgtttgaa
     3721 gtcgtaagta cgtttacggt acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc
     3781 accgtctgac ttcttaatca acgctggatt caggtcataa gcagataggt caacgatctc
     3841 tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa agtttgacga tttcatccat
     3901 cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca gcagatcata
     3961 gattttattg aattctttca tcgattcatc acggaaccat tgccataatt gaatagcctc
     4021 ttcatcgcct tcttccagac gtttgaacca agcacgagct tcttcattta attctggttc
     4081 tgtttcagct acttcatgga attggacata taaacgaagc aattcattga ttggttctgc
     4141 tttaacagct tcttctgtac cccatttttt gtaagcgacg atcagttttc caaactgtgt
     4201 tccccagtca cctaaatggt tgatgcggat tggctgataa ccgatttttt ccataatgaa
     4261 accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc attgagattg gtttggcaat
     4321 attaggagaa gacatgtcga ttggtactgt tccttgattc ccgatattgc tatccccata
     4381 atgttctttt tctttcacta ctgtttgcaa aacttttttg ctgataagtt ctttattcat
     4441 gaaaaagttc aagtaaggtc cgacaacttc gattttttca aagttggcag aatcgatttt
     4501 ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa
     4561 ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta attgttcgac
     4621 ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga caagatcttt
     4681 attattcatc tttattcctc ctaaagtgct cccttgattg ttaaacaaaa aaaatctcta
     4741 gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta ccactccaat
     4801 tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt ttaaaacaga
     4861 attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt ccgaattcgc
     4921 tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat taaactttct
     4981 tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc tgataaaaaa acaatccaga
     5041 tggcctaaag gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag
     5101 acttaacaaa ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga
     5161 tattttctaa agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag
     5221 ctgcctctac tttcggaagc atgcttcctg gagcaaattg cttttcatca atgtattgtt
     5281 tcatttctga aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat
     5341 acacattgtc tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt
     5401 tttctgaagc aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt
     5461 ctacaacagg aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg
     5521 tttcgatgac acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc
     5581 atccacgtcc agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg
     5641 cttcttcttc cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat
     5701 tttcatccac aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct
     5761 tcagtacttc gcccatggca ttttgtaacc agtaaccgat agatccttct gtcattgcaa
     5821 cacatgtatc taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca
     5881 ataggttacc gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga
     5941 taaatttcac taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg
     6001 catccgtaga caagattgca ttacccccta aagcgacaac tacttttcgt ttggccattt
     6061 cattcgtcct ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg
     6121 tgattttccc tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc
     6181 gagggataaa caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat
     6241 tttctgcttg gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca
     6301 tttcagtgat gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga
     6361 aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt
     6421 ccatattgat ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac
     6481 ccattgatac ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat
     6541 cgtcggtaat catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga
     6601 tttcttcttc aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg
     6661 cacctgtgac taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag
     6721 tgataccttc tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg
     6781 ttggatgcca ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt
     6841 cctctaccat tttttgactg aatccacgga attcgattcc atcaaacata cgacctagta
     6901 ctttagctgt atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat
     6961 attcaggatg tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag
     7021 atgttttttc aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac
     7081 gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg
     7141 taaagtcttt ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca
     7201 ttctccttta gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt
     7261 aagaccgccg tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc
     7321 agcgtggacc accacggcct cgtgacaatt cacttgaatt gatttctaac actttgatgc
     7381 catagctacg aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg
     7441 gagcgattgc caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat
     7501 ctccattacc agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt
     7561 cgtgtaagtt atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga
     7621 catccatctt accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta
     7681 ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt gtttggaatt ttgattgcta
     7741 acactttttc aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt
     7801 tcgcattagt tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt
     7861 ctccgccttc gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg
     7921 caaatcttgg atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt
     7981 caaaagtcat cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa
     8041 agtacaagtt tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcgt
     8101 atagactgct agaacgtact tcaatatctt ttgtacgaac cccagccatg attttatcta
     8161 ccatttcttg cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg
     8221 atgcaatatg agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt
     8281 caatcgcttc tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg
     8341 tctttgcaaa gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca
     8401 acagacgatc cataatatct ggtgtcaaat tttccacttc ttgtccaggc cttttcagta
     8461 aaactgtctt taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt
     8521 gcctccttct ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt
     8581 ttgtctcatt caaaatgaga cat
//
LOCUS       DANKHK010000097         8596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000097 DANKHK010000000
VERSION     DANKHK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..105
                     /locus_tag="PW109_002163"
     CDS             <1..105
                     /locus_tag="PW109_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2511962.1"
                     /translation="DFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(173..466)
                     /locus_tag="PW109_002164"
     CDS             complement(173..466)
                     /locus_tag="PW109_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2511963.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(494..1159)
                     /locus_tag="PW109_002165"
     CDS             complement(494..1159)
                     /locus_tag="PW109_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2511964.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1299..1931)
                     /locus_tag="PW109_002166"
     CDS             complement(1299..1931)
                     /locus_tag="PW109_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2511965.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1938..3005)
                     /locus_tag="PW109_002167"
     CDS             complement(1938..3005)
                     /locus_tag="PW109_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2511966.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3002..3733)
                     /locus_tag="PW109_002168"
     CDS             complement(3002..3733)
                     /locus_tag="PW109_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2511967.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3901..4380)
                     /gene="greA"
                     /locus_tag="PW109_002169"
     CDS             complement(3901..4380)
                     /gene="greA"
                     /locus_tag="PW109_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2511968.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="PW109_002170"
     CDS             complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="PW109_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2511969.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5856..7016
                     /locus_tag="PW109_002171"
     CDS             5856..7016
                     /locus_tag="PW109_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2511970.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(7056..8003)
                     /locus_tag="PW109_002172"
     CDS             complement(7056..8003)
                     /locus_tag="PW109_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2511971.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(8036..>8596)
                     /locus_tag="PW109_002173"
     CDS             complement(8036..>8596)
                     /locus_tag="PW109_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2511972.1"
                     /translation="PHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENT
                     DISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVL
                     TSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELV
                     YENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2587 a         1781 c         1334 g         2894 t
ORIGIN      
        1 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
       61 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
      121 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
      181 ttttaaagtg ataggttggc tcctccaggt tgggatcata atcaacaagt aaatcatatc
      241 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatcc atttccactt
      301 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
      361 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
      421 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
      481 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
      541 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
      601 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
      661 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
      721 atttatctga cagctctaag tagtctagaa tattctttga tactaagtta tgtgcaatcc
      781 gtatccccat gtctcgctca gggtgtacga cacgatctgc tccgatcttc tctagtacac
      841 gggcatggta ctcattttgg gcttttgcta aaatattcgg tacgcccatt tcctttacca
      901 tcagagtaac taagatgctg gcttggatat cttctccaat agcgacaatt acatgatcga
      961 aatttcttat acctaatgaa cgaagtgtca tctcatcctg tgcatttgcc acaacagcat
     1021 gggtggctat attcatatat tcattgactc gatcttcatt actgtcaata gccagtactt
     1081 cttgtccagc ttcgatcagt gtacggcaga tgcttccgcc aaatcgtcca aggcctacaa
     1141 ttgcaaaatt ttgtttcacc attttcctcc tttagtatct tcttttctca gtataacaaa
     1201 gcctttccaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
     1261 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgttta
     1321 aacgcataga tagctgcttg agttcggtct tctacctcta acttagctaa tatattcgac
     1381 acatgcgttt tgactgtttt cagtgtgata aacaattcat ccgcgatttc ctgattactt
     1441 ttcccttcag aaatcagcat caaaatttca ttttctcgat tcgttaattc ttcatgcaat
     1501 actggatcat ttcgccgact cattttttcc atcatttttg ttgtcacttc aggttctagt
     1561 actcgctcgc cgcgttgcgt agcccttata gcatcagcta tttcatgtgc tgtagacgtc
     1621 ttcaacaaat atcctgctgc tcctgcttca attgcaggat aaactttttc atcatcaata
     1681 aaacttgtga cgataatgat tttggctttt ggccaatctt ttaagattgc ttttgttgat
     1741 tcgatgccat ccatttcttc catgactaga tccattaaaa tgacatccgg tcgaagtgcc
     1801 atcgcctttt catagccttg acgtccattt tcagcttctc caatcacttc aatgtcttct
     1861 tggatagata aataagaaga gacgcctaag cgtaccattt catggtcatc tactaataaa
     1921 acttttatca tctttatcta tgcctccttc atcaaaggga ccttggtctc cacgctcgta
     1981 ccttgccctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
     2041 atattgttca atccataact tcccgttttg gtttcattca tattaaatcc tgtcccgtcg
     2101 tcaatgatac gtaataaaag attgttatct attttatgca aatagacttc taattcatta
     2161 gccttagcat gtcttaatgt gtttgataac aattcttgaa cgattcggaa caagtgatcc
     2221 tcaatggaac tagttaattt tacatcttct acctcccatt ttagcgaaat ctggatttta
     2281 ttctgcaatt ccttcaataa ctgctcaatg ccttgtttta aacttttttc ttctaaattg
     2341 actggacgca gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
     2401 gccaccattt tcaactgctt acggaacatt tctggtgtct cacttttttc tgcttgctcc
     2461 gtcaatgccg acatcatcat catcgctgca aataattgct gtgaaactga atcatgaagc
     2521 tcacgagcta atcgatgcct ttctaatttt aaaatctctt ctttggtttg tccatctaca
     2581 tattgaggac ggctagtcat aatctgtaat tcactggata cttcgatcat tttttctttt
     2641 attttagtaa tttccatatc aatatctttg atgtacagat catccgacgc gcttgggatc
     2701 ggctgatgca aaagtttgga ttcataattg ccagaagaaa gtgcacgaag cttttcttcg
     2761 attttcccgt attttgcctt ttgaataagt gataacaata gaaatacgat caatcctaca
     2821 cctaatgaaa ttgccatcaa atggaagata agcggaacgt ataataaacg cgcacgcagc
     2881 aattctcccc accaatgact ttggttactg gcataaaaat aagtgaaaag tgaaaataga
     2941 ataataagga aagccgcaat ccccgaataa acagctagca ttgctttgga tattcttccc
     3001 atcatattcg tatcacctcg acatctccaa ccaaagtatt ggtaatgatc ttcaatctgc
     3061 gaggattttc atcataatca ctgctgcaga ttttgatctg ttcgtttttt aaggaaaact
     3121 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaaacga atagccactc
     3181 ccaggggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
     3241 cttttggcaa aagtgtattg cccaaatcga tgatagtatc accagaaatg atggcaatgt
     3301 tgatatcatc ccattcatag acgtcgtttc caatacgttg gttaccaaat aattgctgtc
     3361 ttttacgctc attattatgt gtttttaatt gctctgtttg gaccatcatg atctgttttt
     3421 ttcgccaaaa agcatttttt gtcaaatcta ttcctgaaat ctctacacct ttcagcccga
     3481 taaataaaac ggccaataca accatcatcc ataaagcagg actgttgact aagctaaaaa
     3541 aaatgaccaa gctccctaag atcaattgaa aattttgaaa tttagtacga ggatatttgc
     3601 gcatagctag atatatattg aaaataccga atattactaa aagcaaaagt ccagtattgt
     3661 ttacgatttg ccaaacagca aataacagca gcagtgcttc gactaccaca aaaaaacgcc
     3721 aagaactatt catcgctttt tctccctcct gacacattct catttcatca tacctatttt
     3781 tttgactgtt ggctatagga ctttagatgt aagatcgttc agtgaaacct gcaatactca
     3841 aagagaaaag atctagagtg cgtcatcagt aagatgaact ctctagatct ttcagtcaga
     3901 ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggtg tagcaatggt
     3961 tacttcatca tttttacgtt tgccaatcaa tgcttgcgcg ataggagaat cattggaaat
     4021 ctttccagaa aacgggtctg cttcagcact tccgacaatc gtgtactctt cttcatctcc
     4081 atcaggcaac tcgatgaacg taactgtttt accgatcgat acctcatctg catctacgcc
     4141 gtcattatcg atgatttgtg caaaacggat catattttca attgttgtga ttcttccttc
     4201 aacaaatgct tgttcatctt tagctgattc gtattcggag ttttcggaaa gatctccgaa
     4261 accgcgagcg attttgattc gttcaatgat ttctccgcgt ttaactgttt ttagttcttc
     4321 taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat
     4381 gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt
     4441 ccaaaaaaga ttaccacgct tttcttaaaa tgtaaatgtt tttctaaaaa tgatcaatga
     4501 ctagcccctg tttttctaag aattatttac atatttttgc actagttcca tatgttcttc
     4561 atacgtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc
     4621 attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggacc
     4681 aggcccatag ccggtattgg tatacaaatt gtatggtgaa tctacagctg tatcttcata
     4741 cgtcaccaat tctttatgtt ctcctaatgc atataaaaca gagatatctg actgaagtgg
     4801 catatttgcc ttgattcggt taaagaagac ttgtgcaata tttttacgat cattttcttt
     4861 tacgccttct ttttcaacta gagaagccaa agttaaaact tcttgtacag tcaaattttt
     4921 ctgtttgata gctgaataat agttttgcat gaccgtattt gttttgttca ccatctcaat
     4981 cacaagatcc ttaagcgaag tactcgtgta gtaatcataa gtagctggga ataagtagcc
     5041 ttccaaacgg tattttgtgt tctcagcttt agaggcactt tccaacaatt ctgggaattt
     5101 ttgatacaac tcgttaaaga aagtctcatc atttactaaa tctaaaaatt ctttcttgtc
     5161 tttaccagtt accttcgcta cccgctcagc aatctgatcg atgtcgtagc cttctggaat
     5221 ggcaattttc ccatctgcaa cttttgttgg ctcagatgtg ccgccttctt gcaactgctt
     5281 gccaatctca tcaagagtca tattaggcgc taactgataa taaccagctt gaaagccagt
     5341 taagttttta aattttgtat aataattgaa aacaatccca cttttaataa tattgtcttt
     5401 ctccaaaatt tcgccaatct gcttattaga agagccgctt gggatttcca cttggactaa
     5461 ttggtcgtcc gatttatcta aaggcttcaa cccgctatct acataacgat atacggtaaa
     5521 cccgagaaat ccgccaataa tcaataatgt aagagcgatc actaaaatga tcttcctcac
     5581 gatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc
     5641 cttactggct tcttttttgt ctttgcttgg gtgttgtttg tttcctgaca aaacaactcc
     5701 tccttactat catcttcgta actctgttta ttatacatga aatttctact gccgtgaatt
     5761 attttaccaa atattatgtc aaaattgtaa gtggcaaatg ctctttttga ggattacaat
     5821 gaaaaatagt taaaaatgga taggaaggat ttaatatggc aaaaggaaaa catcaaaaaa
     5881 aacagcagat ctctctttct tttctcgcag ttttgttgct ggctgtcagc ttcttttcaa
     5941 caggcatcat tacaggatgg caattaacaa aacaacctca accacaaatt tataaaacgg
     6001 aacccgcaca acttgcttcc aatttaacat ctaatgagca gatgcttgcc agcaaaattg
     6061 atcaattact gcaaaaatca gattatatcg gttcgattta tgtacgcaaa aataatcgag
     6121 tgatattaga aaaaggatat gggtacgcaa atctacgcat aaaagaatcg aattctcctt
     6181 ctctttacta tcaaatcggt tcgatccaaa aagcgatgac tgctttactt atcctaaaac
     6241 aaattcaatc tggcaagata acttttgaca caaagctatc tgaattttat cctcagattc
     6301 ctggaagtca gcaaatctca atcaaaaata tgctttacat gcgctctggt cttcatcgaa
     6361 ccgcttctcc aaaaaaacct atgtcagata gtgagatcat tcagtttgcc cttcatcatt
     6421 tgaaatttac tggttatgat acgtatcatt atgaaccatt aaattataca ttgcttacag
     6481 gggtattgat caaactaatg aatcagcctt atgaaactct tttaaaaaaa gaaatcattc
     6541 ggccactcca tctagaacat acagattttt atgaaaatgt aaaaaattcc cctcagcatg
     6601 ctgtatctta ccaaatgtct gcaactgacg attattcaaa agcattggtc gagcctgaaa
     6661 cagacatccg aaatgaatta ggcacaggaa atataagtat gtctgtttat gacttgaaca
     6721 aattctttac cagtgttctg tctggcgata ttataccaaa agaactactt ttctcattgt
     6781 ggcaaaataa tggtgataaa catccttaca gcggcggtgt ttacagtgga aatgattata
     6841 tccttgctca gggaaatatc aatcgattcc atgcagtagc ggcgatgaaa aaagacacaa
     6901 aagatgcaat cgtcatggaa agtaatatcc aatctgataa aaacatcaaa ttgccggcca
     6961 ctgatttaag aaatcagatt tacgaactga tggaaggtgt taatttaaaa agctaagtcg
     7021 agacaaaaaa accagcagga tgctggtttt ttgtttcaat cgatttctgt ctttaaataa
     7081 cgttccattc cgttcaatat atcaaatttg ggttgataac ctaaagaacg gatctttgaa
     7141 atatccgcta aagaatcacg aatatcccct gaacgctccg cttcatattt aagcgctagc
     7201 tctgttccta agatttgatc aattgaatga atcaattcaa gtagcgtagt gctttttcct
     7261 gtaccgacat tgaattgctg tcctaaagct ttttcttcct tagcaacaag taaaagtgct
     7321 tgtataacat cttctatata aacgaagtct ctagattggc taccatcacc aaataaagta
     7381 aattctgttt tttctcctgc caattgtttt ttgtatcggt ccactagaat cgaaataact
     7441 ccgctatatg gagagttagg attttgattt ggaccataaa cgttaaaaaa tcggacagca
     7501 ctagtcggaa catcatataa atgacagtaa tccaaaacat attgttctgc tgcaaactta
     7561 tcgatggcat atggtgttaa aggacggatg accgattctt ctttttttgg caacgttggt
     7621 tcatcgccat aaactgcagc tgatgaacta aataccaatc gttttaaacc agattgatat
     7681 tttcgtacca attcaagcaa taaaagcgca ctgtcaaaat ttacacgatg agtttctaga
     7741 ggacgttcta cagaatctgc cacactagct acagcagcta gatggaaaat ataatcgaaa
     7801 tggtattctt tcatgacctt ttccatcaaa tttttgtcag cgacatcccc ttcaataaac
     7861 gtgatgtgct tcgattcatc cagattctct tttttcccca tcgataaatc atcgatcaca
     7921 actacattgt tttcttttcc tagataattc gcgagcgtcg agccaatgaa gccggcgcca
     7981 ccggtaatca agtaattcaa cattttttct cctcttttca agataaaatt tattactaaa
     8041 aatagctaga attatttttg ctattaactc ttcctaaaaa tagccaaaaa acaaacgttc
     8101 ctacagtgtt ttcatagact aattcattgt taagagctgc gtatagtgca taagataaaa
     8161 taactaaaca gtaaacaaaa tgataatctt taccaatgca tttattatag atgattcgat
     8221 aaacattcag cacaaaactc tttattaaaa aagccatgaa aggaattatt cctagaatac
     8281 ctgtcgatgt taatacattc aagtatgcat tatgaatggc aattcctact ttcgcaatat
     8341 atccatctgg aataattgac tgtgcataag atactacatt tttcggagag actccgataa
     8401 gccaacttgt tttaaatatt tcccatgcac ttttccatat agtaattcta gcattagaaa
     8461 tatctgtatt ttcagctacg tctttcctaa caagagtatt atcaggtgtt tgagtattat
     8521 taatatgttg actattattc attaattctt tatgacgctc tgtaaaatta aaaaaattta
     8581 tttcttttaa atgagg
//
LOCUS       DANKHK010000098         8545 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000098 DANKHK010000000
VERSION     DANKHK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(146..289)
                     /locus_tag="PW109_002174"
     CDS             complement(146..289)
                     /locus_tag="PW109_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511973.1"
                     /translation="MKKVYQKCLWISYITLGIAVKEMGENINHDSNTNIKHIKHLTLL
                     LSG"
     gene            complement(307..537)
                     /locus_tag="PW109_002175"
     CDS             complement(307..537)
                     /locus_tag="PW109_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511974.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVHLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            complement(560..1366)
                     /locus_tag="PW109_002176"
     CDS             complement(560..1366)
                     /locus_tag="PW109_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511975.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSSLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(1377..2021)
                     /locus_tag="PW109_002177"
     CDS             complement(1377..2021)
                     /locus_tag="PW109_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511976.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(2037..3242)
                     /locus_tag="PW109_002178"
     CDS             complement(2037..3242)
                     /locus_tag="PW109_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2511977.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGETFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHQGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(3242..5266)
                     /locus_tag="PW109_002179"
     CDS             complement(3242..5266)
                     /locus_tag="PW109_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2511978.1"
                     /translation="MLEAIKIAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(5429..6022)
                     /locus_tag="PW109_002180"
     CDS             complement(5429..6022)
                     /locus_tag="PW109_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511979.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRVEQERPDLSFFFIIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYEKELIKILYRMNNMNDE"
     gene            complement(6023..6355)
                     /locus_tag="PW109_002181"
     CDS             complement(6023..6355)
                     /locus_tag="PW109_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511980.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(6407..>8545)
                     /locus_tag="PW109_002182"
     CDS             complement(6407..>8545)
                     /locus_tag="PW109_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511981.1"
                     /translation="ELFQSLISKVLALFCVVTLIYLGLKFFMSKHPPKIKNILVNLFM
                     AMILIFGGSSLIDEGLNISKNFYGDVTAANKADKTGSPAFQIIQNNVIDVSTLLETDP
                     NKVESLPTDKRNALTAKNFKTANINSVIIPKEAAKMADEDKSGLSDIQKDRLRALQYR
                     LELDDNGKEIPVEISDEGLAKYVYTSGYRRYISSPGIILAGESSLSVAYLFILFTIVT
                     CIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILELRIYTLMLSGIG
                     DLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNSLLSMFALGNIA
                     KNVGSGAKNLASAGKSGLSNLNDIRKNGFGNRKDVTPDNGEEQSDDKATTVPNSKKTI
                     SEMFGKAKNGVSKAAEGLGYVNERGLLKGTAEDLTEKAKDTLEEKLDGTRAKELAEAV
                     KNPSEALEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTSEKENQEGAAIPSAKDHQN
                     ELHSNENSIGEKDSEEGQHGQLPKTDPEQYRYKSNINSENEKRISDLPKRNQEVQTDE
                     DSIDLPNDNGIEVGTEKSMKIGLPKTQNLPMKDSRLNQFKENDQSFVHQSTAGTVQMK
                     PSRLPENGNLTTVSYNLSESGETGNVSVQNVVTREAAPEPIQKVHTGYKPANVAFTAP
                     TKINVTRETKNRNNELKEQLLRDIRQDIKP"
BASE COUNT         2291 a         1794 c         1374 g         3086 t
ORIGIN      
        1 aaattaaggt tatttgtgta tttagacaat aaaaaatcct tctaactttg tcgattagaa
       61 ggaaatattt tgtcatatga aaaatgaatt taagaaaaag tgtatgctat aattgatacg
      121 ctgtcttcct gtttggttga aatcttcagc cagaaaggag gagtgttaaa tgttttatgt
      181 gcttgatgtt tgtgttagaa tcgtggttaa tgttttctcc catctcttta acagctatac
      241 ctaaggtgat atagctgatc cataaacatt tttgatatac ttttttcaca taaaatcctc
      301 ctgtttttat tttctttcag ccgtaaaata ttttttagca gctaaataca aagcttcaaa
      361 tgatctgaat tttaaggtat ctaaactctt tctaccattc ttcaactcag atattgttgt
      421 gttagataaa tgcacttctt gacatatttc tttagctttt acaactttga gataggcttt
      481 aatcaatact ttctgctcag cgaaagtatc tttgccgacc aagttcgctc gcttcatttc
      541 ttttactcca cctattcttc taaatatttt cttttgtcat acgatcaatt ttttcttgaa
      601 gtttttgaat gatctcttct tgatctttca tttgcgtttt cgatgtttca atttcttgct
      661 ggtaactatt aatcttattt tcagtatttt cctgatcgtc tgcaacttga taaggcaatt
      721 gatcttttag tttcttcact aattcttcat tcaaatcaat ggttgcttgg aaggattcaa
      781 tttgtttttg tgctgctttt tgttctttct tgtatccttt cactttgtaa ttgatcgcat
      841 gttgttcata atcttcatct ttcacttgat ctttaacctt atcttcgtga acgtaataaa
      901 tcagatcttg tggttcttgc atatccactt taggatcaat tttttcagga ataatggtta
      961 aacgcatggc gatatattct tctggtacat gtgctaactt cactacaaaa taatcatcag
     1021 acaccttgat cactttggct tttattgctt gataatcccc tttagctgtc tttacttcta
     1081 cgcgataatt aagattagcc agttctttta atgagctttg atcttcatcg tcgatcgcaa
     1141 attcaaaaac taattgctgg ttcgctcctt tttcttttga aaccatcgtc agttccttcg
     1201 tgtttaaaaa tgcgttcgtg cttacttcat ctggtatgtc taccttttta tgtgtgtaaa
     1261 tcggactagc aagaatcaaa aagccgagaa tcataaagat tcccagccat ctaagataac
     1321 ttttccgttt tttgaaacgt tgtcttgctc cttttttctt actcattttc ttcctcctaa
     1381 cgagtgaaat aactaaagtc agtgactttc cattgaccat ctacctgttt ttcagtgaca
     1441 gttaatccac cttttatttt ttgtggtttg acatctttga attgaggatt tccaattgta
     1501 taatcaacaa ataccacaaa gttttgttcc tcttttacac tgcttgtttg agcataagtt
     1561 tgcacgtcac ctaatgtaaa agagtaactt ccgtaatcat catgttggtg atcatcgtct
     1621 gcaggactta atttgtcgat cacttcttgg gttgcgacat cttttagttt ttccgttaat
     1681 tcatttctgc ttaatgattt atcaatggcc aattgaccga actcttctgc cgtctttctt
     1741 tgtgcttcag tgacttcctc ttttgctttt gtgccatctt gttgcttcag ctgatcgatt
     1801 tcttggtttt tctcattgat ggtttgattt aacttgtcga tcttcgcctg tgcctcagag
     1861 gaattttttt tgagcttctc agacgcgttt ttagccactt cttcaatttt aaaggtattt
     1921 gtctcttgtt cttctttcag ctgccaaaac aacgcgatat tgccgattaa actaacgcta
     1981 attgcagctg ttaagacgat ttttaaagtt ttattttcca tattttttct cctgatctat
     2041 tttttgctgc tattcaaaaa tggtaatgga tcaatccatg taccatcatc tgtgaatgcg
     2101 tgtccttgtg cttgacgcca atccattcga gtgaaaccta aatgcaagtg tgctgtatct
     2161 cggatcccga ttacttgacc aactttcacc tgatctccaa ccttcacacg agaatttcct
     2221 gtactatttg caaattcttg atataccata ttcaagccgt cgtaattaat cacgatcaca
     2281 cacgcgccca aacctgaaat accaggattt ccgacgtaca cgacttttcc tccatgaacc
     2341 gcatgaatct cacttccttg atgatcaact gaaccaaagt ctaatccatc atggaaacca
     2401 tttggtcgga attcaccgcc tggatttgtt ccaaataatt gaccaccact gaaggaactt
     2461 ttatctaaac tgctgccagg gaatggattc gtccattcac cagtgatcgt gccgttgtta
     2521 ttgccacctt caccccaatg tttgatggcg aattcatcat attcggttaa tttattagaa
     2581 gtgataatgg acatacaacc tgtataatac ggactatctt ccgtaattcc tgcagcggca
     2641 taaccccctc gtcctaagat caataagctt gcgccaggac ttttatttcc tacacaagca
     2701 ttgaaattgg attgtgtggc taccataaag ccgtaatagg agaaggcagc ttttgagttg
     2761 ggaaacttca tgtaccagcc accttcaatg ccaccaattc cacgcgcagt tccttttgga
     2821 aataaacagc catcgaacca agtaatccca aaataattga gatcatttct tgcagacgcc
     2881 gacgtaccaa acgctgattc atacccgtat tgcgaaagga tcacacttgg taataattta
     2941 tacttcactg ctgcacctac ggcatattgc gcaatctcat cagaaaaagg gcctcttcct
     3001 ttgatttctt tataaatggg caatccttcc gaatactctc ctgtaaaagt ttcgccacca
     3061 ctgcttgaat ctgaatcaga attatcaatc gaaccagccg caagtattaa aaatagggtc
     3121 acgaatacag tgatcaaaat ggtaatcaaa cctgcagaag taccaaacaa ccacaaaaat
     3181 aagcgtcgct ttgcacgacg cttaacatac tgcttgatta aactttcatt ttggttttcc
     3241 attattttcc tccatcaaac aattttaatt gacgtttgtt cggactaaaa tgaacacgaa
     3301 tggtttcttt tgagccaaaa ttgatcacag cgtctccttg attttgttga ggaatggatt
     3361 ctaattctga gttcgtcatt gatttaccaa ctaatccttc tatatgacgt aaaatagatt
     3421 catccatacg cataaagatc ttcgtagaag aaaattcaaa gactttcttc agtttcgaga
     3481 agttatcaga agacatattt tctggcacca tttcttgtgg ggattgtgta gctaaaacag
     3541 tcattgcttc tactttacgc atttctcgtt gaaagtttgt gacataattg accgtttcaa
     3601 taaaatctgg attaatcgca ttatggcact catcaatcat cgccaagaag cgtttgaagt
     3661 cttgttcttc catttgtttt tctttaatta aacgtcgttg tcttcttcct tgaatcaatg
     3721 cctgattcca aatcaacgtc atagccatgt acatctgaca ttgttgtacc ccacgatcta
     3781 aacctgtaat agagtcaatg tcaaaaaaga ccaactgttg ttggtcaagg tgtcgaatcg
     3841 tactgtgacc atcaaaaatc atgccatacg tctcacataa ggattctacg atggattcca
     3901 tttcttcttt tcgattcttt ttctcttggg aagcacctgg taattccagt tgtcctaaaa
     3961 agtctaaaaa ttctttgaac gtcggataag cttctggatc tttccctgtg acttgcgtct
     4021 ccgtatttga acgcgaattt gaggaagtaa agccgcgtgg tactgttcca tactcttcat
     4081 aaaaacgatt taagtaggtc ttaaattctg ctttcatcgt tccttttagc tgaggactca
     4141 ctaactgtac ctgatagacc actttgtcta agtgtgtttg ataggacgct gcttcattga
     4201 tcttcaaatc tttgactgat ccatcgtcat ataaactgga tgcttcatca tacgtccgtg
     4261 tggcaaatat ttctaaagga ttgatcatcc cgtcagatcc gtctaagcgc accataatac
     4321 cgttatattc ctttaagaaa ggaataaagt cttttccctt ttcaaagccc caaataaagt
     4381 ctcctgcagc taaatgggct tccgcgatca ttttaagaac ggtcgattta ccagctccca
     4441 tcattcctag aagcaaggta ttagtagaca accgtacact atttttatac gtcgcattcc
     4501 agacaaatgg gccgcctgtc atggtttccc catattgtgt ccctcctgga tcttttagcg
     4561 tttggtggtg gaacgggaag cctccgccaa tgtttaacga tggtagtgat tgccctttga
     4621 tatttccgaa ccattcagct tgttcatcga acatggtaaa catcgattgc caatctcgtt
     4681 tggttttatg cagataggtc accgcacgat aatccatccc tttgagattt tcaatcagtt
     4741 cctgcgctcg ttcatctact tcttgaatcg tgtttccgta aacatgtact ctcactttga
     4801 cttgtttaga aatttcccca ttagaagtca attttcgggc atattccgct agttccaggt
     4861 attcttctgc gcttttattt cggtcagtcg cttttcgttc gttatctgat cgatcattta
     4921 attcatctaa ggcacgattg atttgatcaa tcactttttg cttttcatct gtgctaacat
     4981 cgagtgtggc cactgtatga ggattgttca tgagattggc caaccaaaaa agcgtaggat
     5041 tttttgtgta gtgataaact gtcacacacc gtgaataacc atttccatag gtcacatact
     5101 cttcatcgaa atttaatcca ccttgtggca tcacttgttt taaaaaatct aaatcatacc
     5161 ctttggattt taaaatttct atttcccgat ttcctaattg ctttaatgga tcaagtactt
     5221 tttgatcatt ttgtggtgaa aaagctattt taatcgcttc tagcatgtac aatcacctct
     5281 tcttgtttga ttctttactt ggttaaaaga ttgccctttt cccaacttac ccaggcttaa
     5341 agtgtgagga aattttctag tggtctcttc cttcggaaca tctttttcta accatatcaa
     5401 cggatatttc attctctctt ctccattcct actcatcatt catattgttc attcgataaa
     5461 gaatcttgat caattctttt tcgtagagtg gttttaaccc taaaatgcca ctagatcgca
     5521 ttaactgacg gtttctggtt tgtaaatcct tgattgtctt tccttcaatg atgaaaaaga
     5581 agcttaaatc aggacgttcc tgttctactc gtttcaattc ctggatttcc tctactaatt
     5641 tcttttcata ttttcttaat cgctgcagat ttcttcctaa ggactgttcc atacgatttt
     5701 tatctaataa ctgccgcttt tgcacgattt gttccgttag atccgtctct gtggtcaaac
     5761 taactaactt aattgttggc acatacgtac gcatgacggt ggtcaactga ttcattcgtt
     5821 ctagtttctc ttgttcattt aatccttcca cgtctgtaga aactacacga taataacgtt
     5881 gaaaatgatc ttctttagta actaagactc cctcttctgt acaggattta taaggaaata
     5941 aatccaagat agatcgttct tctggttttc caaactgaaa aatggatttc ttatcaactg
     6001 aattttgtcc tttaactcgc atttattcat cctccttata cgtaccatga taatttcggt
     6061 taggatctaa cgcatgataa tggttcttgt ccgtaaaaaa aatagactcg ataatcgcaa
     6121 aaatttgtcg ttgccctggg ttattatagg gtcgccaaac aatcactaga gctatcgcaa
     6181 tccataagcc ataattcaag agtgcaaaaa agagcggtaa gtggacaaga ttattgacta
     6241 ttttcgccca aaaaaagcaa ccaccaagcg ttaatagatc taaaaagaaa aaggcgaaaa
     6301 acttccattc cccactaaac cttgggggat ttttcatgtt aatgtattta tccataaatg
     6361 atttctcctt ttcatacata gaaaaaaggc ctaacttgta ggccttttat ggtttgatat
     6421 cctgtctgat atccctcaat agttgttctt tcaattcatt gttcctgttt ttagtttctc
     6481 ttgttacatt tatttttgtt ggtgcagtaa atgccacatt tgctggtttg taacctgtgt
     6541 gaactttttg aataggttct ggtgcagctt ctcttgtcac aacattttga acggacacat
     6601 tccctgtctc accactttca gataaattgt aagaaacagt tgtcagattt ccattttcag
     6661 gcaaacgact tggtttcatt tgaacagtac ctgcagttga ttggtgaaca aagctttgat
     6721 cattctcctt gaattgattc aaacgactat ctttcattgg taaattttgt gtcttaggca
     6781 aaccaatttt cattgatttt tcagttccaa cttcgattcc attatcattt ggcaaatcga
     6841 tggaatcctc atccgtttgt acttcttgat ttcgttttgg taaatctgaa atgcgctttt
     6901 cattttcaga attaatgtta gatttataac gatattgttc tggatcggtt tttggtaact
     6961 gtccatgttg tccttcttct gaatcttttt ctccgatcga attttcgtta gaatgtaact
     7021 cattttggtg atctttggct gaaggaatcg ctgcaccttc ttgattttct ttttcacttg
     7081 ttgtatttgc cttcactgct gcattgactt gtccttcacc atacgcatct tttagtcctt
     7141 ctttgacttc tgaagctttt tctttttgtt tttctagtgc ttcggatgga ttcttcacag
     7201 cttcagccaa ttctttagct cttgtgccgt ctaatttctc ttctaaggtg tctttggctt
     7261 tttctgtaag atcttcagct gttcctttta acaacccacg ttcattgacg taaccaagac
     7321 cttctgcggc tttagaaaca ccatttttcg ctttcccgaa catttcggaa attgttttct
     7381 tactattagg tacagttgta gctttatcgt ctgattgttc ttccccgtta tcaggggtaa
     7441 catcttttcg attgccaaat ccatttttac gaatatcatt taaattgctt aaaccactct
     7501 taccagcact tgcaaggttt ttagctccgc tacccacatt tttcgcaata tttcctaagg
     7561 caaacatgct aagaagagaa ttgctgccgt ttttcagact tgtatcaaca ccgaaaatct
     7621 tcgtaacaga ttgagatcct ttgaatagcg caatggttag tacaattaaa gcgacaacat
     7681 acaaaaatcc gttgatcttg cctgctgcgt gcaaatcacc gatacctgac agcattaatg
     7741 tatagattcg taattctaaa atcgtaaacg ccaataacaa gaaggattga gaaatatctt
     7801 ctatagcact tttcatccgt tgtcctgtct caaagtccgt tgacgcagca attactaaat
     7861 agaatttttt aaataccagc tcaataatgc aagttacaat cgtaaacaag ataaataaat
     7921 aggcaaccga caaagaagat tcaccagcta aaataatccc tggactacta atgtatcggc
     7981 gatagcctga agtatacaca tattttgcta acccctcatc actaatctct acagggattt
     8041 cttttccatt atcatccagt tccagtcggt attgtaacgc tcttagccga tccttttgta
     8101 tatctgatag accgctctta tcttcatccg ccatcttagc ggcttcctta ggaataatca
     8161 ctgaattaat atttgctgtc ttaaagtttt tcgccgtgag agcatttctt ttatctgttg
     8221 gtagggattc tactttattc ggatcagttt ctagtagcgt actcacatcg atcacattat
     8281 tttgaataat ttgaaaagca ggtgatccag ttttatctgc tttatttgct gcagtcacgt
     8341 ctccgtaaaa gtttttacta atatttagcc cttcatcgat taaagaacta ccaccaaaaa
     8401 tcaaaatcat ggccataaat agattaacca agatattttt gatttttggt ggatgtttac
     8461 tcataaaaaa ttttaagcct aaataaatta atgtgacgac acaaaaaaga gccaatacct
     8521 tactaatcaa actttgaaac agttc
//
LOCUS       DANKHK010000099         8531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000099 DANKHK010000000
VERSION     DANKHK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..203)
                     /locus_tag="PW109_002183"
     CDS             complement(<1..203)
                     /locus_tag="PW109_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2511982.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRT"
     gene            631..1554
                     /gene="truB"
                     /locus_tag="PW109_002184"
     CDS             631..1554
                     /gene="truB"
                     /locus_tag="PW109_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2511983.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1558..2499
                     /gene="ribF"
                     /locus_tag="PW109_002185"
     CDS             1558..2499
                     /gene="ribF"
                     /locus_tag="PW109_002185"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2511984.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2894..3679
                     /locus_tag="PW109_002186"
     CDS             2894..3679
                     /locus_tag="PW109_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2511985.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3692..4486
                     /locus_tag="PW109_002187"
     CDS             3692..4486
                     /locus_tag="PW109_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2511986.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(4666..5118)
                     /locus_tag="PW109_002188"
     CDS             complement(4666..5118)
                     /locus_tag="PW109_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2511987.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5583..5951)
                     /locus_tag="PW109_002189"
     CDS             complement(5583..5951)
                     /locus_tag="PW109_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2511988.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            6101..6583
                     /locus_tag="PW109_002190"
     CDS             6101..6583
                     /locus_tag="PW109_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2511989.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMTANEKNN
                     "
     gene            6620..7630
                     /locus_tag="PW109_002191"
     CDS             6620..7630
                     /locus_tag="PW109_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2511990.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7745..8458)
                     /locus_tag="PW109_002192"
     CDS             complement(7745..8458)
                     /locus_tag="PW109_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2511991.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2861 a         1390 c         1725 g         2555 t
ORIGIN      
        1 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
       61 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      121 gcaacgatag caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      181 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      241 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      301 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
      361 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      421 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      481 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      541 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      601 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      661 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      721 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      781 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      841 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      901 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      961 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
     1021 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
     1081 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
     1141 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
     1201 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
     1261 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1321 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1381 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1441 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1501 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1561 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1621 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1681 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1741 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1801 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1861 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1921 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1981 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     2041 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     2101 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     2161 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     2221 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     2281 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2341 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2401 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2461 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2521 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2581 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2641 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2701 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2761 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2821 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2881 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2941 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     3001 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     3061 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     3121 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     3181 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     3241 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3301 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3361 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3421 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3481 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3541 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3601 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3661 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3721 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3781 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3841 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3901 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3961 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     4021 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     4081 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     4141 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     4201 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     4261 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4321 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4381 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4441 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aattaacgat tatcggatta
     4501 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
     4561 ctcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
     4621 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaagtga
     4681 aatatctgtc tgcatcaata atttttcatt gatccattga ctaaaattta cgacatgatt
     4741 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
     4801 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggatttg
     4861 attgattgct gtaaaatcac gatactgaag gttgaaacgc atcgtgattg gtgcatttag
     4921 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
     4981 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
     5041 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
     5101 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
     5161 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
     5221 gtcagaaaat tctaactttt ctttctctaa agaaattcgt tctctcctaa agtgatatta
     5281 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
     5341 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagtg
     5401 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
     5461 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
     5521 caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
     5581 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
     5641 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
     5701 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
     5761 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
     5821 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
     5881 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat gatcggctga
     5941 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
     6001 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
     6061 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
     6121 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
     6181 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
     6241 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
     6301 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     6361 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     6421 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     6481 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     6541 gatgccgata tttttgatga cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     6601 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     6661 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     6721 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     6781 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     6841 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
     6901 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     6961 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
     7021 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     7081 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
     7141 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     7201 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     7261 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     7321 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     7381 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     7441 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     7501 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     7561 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     7621 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     7681 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     7741 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     7801 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     7861 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     7921 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     7981 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     8041 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     8101 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     8161 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     8221 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     8281 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     8341 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     8401 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     8461 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     8521 aaaccttgtc t
//
LOCUS       DANKHK010000100         7933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000100 DANKHK010000000
VERSION     DANKHK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..668
                     /locus_tag="PW109_002193"
     CDS             <1..668
                     /locus_tag="PW109_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2511992.1"
                     /translation="KYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFE
                     LAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQA
                     IMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLS
                     EAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGR
                     IKQ"
     gene            665..1597
                     /locus_tag="PW109_002194"
     CDS             665..1597
                     /locus_tag="PW109_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2511993.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            1842..1958
                     /locus_tag="PW109_002195"
     CDS             1842..1958
                     /locus_tag="PW109_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2511994.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1974..3893
                     /gene="tet(M)"
                     /locus_tag="PW109_002196"
     CDS             1974..3893
                     /gene="tet(M)"
                     /locus_tag="PW109_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2511995.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4009..4053
                     /locus_tag="PW109_002197"
                     /pseudo
     CDS             4009..4053
                     /locus_tag="PW109_002197"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            4087..5463
                     /gene="tet(L)"
                     /locus_tag="PW109_002198"
     CDS             4087..5463
                     /gene="tet(L)"
                     /locus_tag="PW109_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDL2511996.1"
                     /translation="MNTSFSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANYF
                     NKTPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSLIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVIVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWYYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            6027..7289
                     /locus_tag="PW109_002199"
                     /pseudo
     CDS             6027..7289
                     /locus_tag="PW109_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            7534..>7933
                     /locus_tag="PW109_002200"
     CDS             7534..>7933
                     /locus_tag="PW109_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDL2511997.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNK"
BASE COUNT         2690 a         1206 c         1636 g         2401 t
ORIGIN      
        1 tgaagtattt cagtgaatta ttagccagta gcgaaaggct cagtgtagat ttagaatcgg
       61 ttatccagtc ctacaattat ggtggtggtt tcttagggta tgtggctaat cgtggaaata
      121 aatatacctt tgaactggct caaagtttct caaaagagta ttcaggtggc gaaaaagtgt
      181 cttaccccaa tcccatagcc atacctatca atgggggctg gcgatacaac tatggcaata
      241 tgttttatgt gcaactggta acgcagtatc ttgtcacaac agagtttgat gatgatacgg
      301 tacaagccat catggacgaa gcactgaaat atgagggctg gcgatacgtt tacggtggag
      361 cttccccgac tacttctttt gattgtagcg gactgacaca atggacgtat ggaaaagctg
      421 gaattaactt accacgaacc gcacaacagc aatatgatgt gacccagcat atcccactat
      481 cggaagcaca agctggcgat ttggttttct ttcattctac ctataacgct ggctcttata
      541 ttactcatgt tgggatatac cttggcaata accgtatgtt tcatgcaggc gacccaatcg
      601 gttatgccga cttaacaagc ccctactggc aacagcattt agtgggagca ggacgaatca
      661 aacaatgaga aaggaagatt taatgatgaa atttagaaaa aatcagaata aagaaaaaca
      721 gataccaaag gaaaagaaac ctcgtgtcta taaggtcaat cctcataaaa aggttgtgat
      781 tgccttgtgg gtacttttag ggcttagttt cagctttgcg atattcaagc actttacagc
      841 tatagatact catactattc acgaaacaac tatcatagaa aaggaatacg ttgatactca
      901 tcatgtagaa aattttgtag agaactttgc gaaagtctac tattcatggg agcaatccga
      961 taagtccatt gataatcgaa tggaaagtct aaaaggctat ctgacagatg aacttcaagc
     1021 tctcaatgtt gatacagtac gcaaagatat tcctgtatcg tcttctgtaa gaggatttca
     1081 gatatggacg gtagagccaa ctggcgacaa tgagtttaat gtaacctaca gtgtagacca
     1141 gctcattaca gagggagaaa atacaaagac cgtccactct gcttatatag tgagtgtcta
     1201 tgtagatggt tctggaaata tggtactggt taagaatccg accattacca acatacctaa
     1261 gaaatcaagt tataaaccaa aagccattga aagtgagggg acggttgatt ccattacaac
     1321 caatgaaatc aatgagtttt taacgacgtt cttcaagctc tatcctacag cgacagccag
     1381 tgaactttcc tactatgtga atgacgggat attaaaacca atcggaaaag agtacatctt
     1441 tcaagaactg gtaaatccta ttcacaatcg taaggataat caagtcacgg tatcgctgac
     1501 agtggagtat atcgaccagc agaccaaagc aacgcaggta tctcaatttg atttggtact
     1561 tgaaaagaac gggagtaatt ggaagattgt aaaataacaa atattggtac atgattacag
     1621 atactttgta atcatgtact ctttttgata aaaaattgga gattccttta caaatatgct
     1681 cttacgtgct attatttaag tgactattta aaaggagtta ataaatatgc ggcaaggtat
     1741 tcttaaataa actgtcaatt tgatagcagg aacaaataat tagatgtcct tttttaggag
     1801 ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta aagtatccag
     1861 agaatatctg tatgctttgt atacctatgg ttatgcataa aaatcccagt gataaaagta
     1921 tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa
     1981 ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct
     2041 tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga
     2101 cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt
     2161 ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag
     2221 cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag
     2281 atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca
     2341 caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg
     2401 atattaaaga gaaactttct atggaaatta taatcaaaca gaaagtagag ctgcacccta
     2461 atatgtgtgt gatgagctgt acggaacctg agcaatggga tgtggtaata gaaggaaatg
     2521 atgacctttt agagaaatat atgtccggta aatcattaga agcattagaa ctcgaacaag
     2581 aggaaatcag aagatttcag aattgctcct tgtaccctgt ttatcatgga agcgcaaaaa
     2641 gcaacatagg gattgagcag cttatagaag tgataacgaa taaattttat tcatcaacat
     2701 acagaaagaa gtctgaactt tgcggaaatg tcttcaaaat tgaatattcg gaagaaagac
     2761 aacgtcttgc atatgtacgc ctttatggcg gaatcctgca tttgcgggat tcggttagaa
     2821 tatcggaaaa ggaaaaaata aaaattacag aaatgtatac ttcaataaat ggtgaattat
     2881 gtaaaattga taaggcttat tccggggaaa ttgttatttt gcaaaatgag tttttgaagc
     2941 taaatagtgt tcttggagat acaaagctat tgccacagag agagagaatt gaaaatccgc
     3001 tccctctgct gcaaacaact gttgaaccga gcaaacctca acaaagggaa atgttacttg
     3061 atgcactttt agaaatctcc gacagtgacc cgcttctaca atattatgtg gattctacga
     3121 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3181 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3241 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     3301 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     3361 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     3421 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     3481 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     3541 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     3601 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     3661 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     3721 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     3781 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     3841 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     3901 tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat
     3961 ttgaattaaa gtgtaaagga ggaattaatt atgaagtgta attaatgtaa cagggttcaa
     4021 ttaaaagagg gaagcgtatc attaacccta taaactacgt ctgccctcat tattggaggt
     4081 tgaaatgtga atacatcctt ttcacaatcg aatttacgac acaaccaaat tttaatttgg
     4141 ctttgcattt tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct
     4201 gatattgcaa attattttaa taaaacacct gcgagtacaa actgggtgaa cacagccttt
     4261 atgttaacct tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc
     4321 aaaaggttac tcctatttgg aattataata aattgtttcg ggtcgttaat tgggtttgtt
     4381 ggccattctt tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct
     4441 gcatttccag cactcgtaat ggttatagtt gcgcgctata ttccaaagga aaataggggt
     4501 aaagcatttg gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt
     4561 ggtggaatga tagcccatta tattcattgg tactatcttc tactcattcc tatgataaca
     4621 attatcactg ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat
     4681 tttgatatca aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca
     4741 acatcatata gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa
     4801 catatcagga aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt
     4861 atgattggag ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg
     4921 gttccttata tgatgaaaga tgtttaccag ctaagtactg ccgaaatcgg aagtgtaatt
     4981 attttccctg gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat
     5041 agaagaggtc ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta
     5101 actgcttcct ttcttttaga aacaacatca tggttcatga caattataat cgtatttgtt
     5161 ttaggtgggc tttcgttcac caaaacagtt atatcaacaa ttgtttcaag tagcttgaaa
     5221 cagcaggaag ctggtgctgg aatgagtttg cttaacttta ccagcttttt atcagaggga
     5281 acaggtattg caattgtagg tggtttatta tccataccct tacttgatca aaggttgtta
     5341 cctatggaag ttgatcagtc aacttatctg tatagtaatt tgttattact tttttcagga
     5401 atcattgtca ttagttggct ggttaccttg aatgtatata aacattctca aagggatttc
     5461 taaatcgtta agggatcaac tttgggagag agttcaaaat tgatcctttt tttataacag
     5521 gaattcaaat ctttttgttc cattaaaggg cgcgattgct gaataaaaga tacgagagac
     5581 ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac
     5641 aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat
     5701 aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac
     5761 ctgtcccttg ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca
     5821 gagggcaggt ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta
     5881 tggttttggt cggcactgcc gacagcctcg cagagcacac actttatgaa tataaagtat
     5941 agtgtgttat actttacttg gaagtggttg ccggaaagag cgaaaatgcc tcacatttgt
     6001 gccacctaaa aaggagcgat ttacatatga gttatgcagt ttgtagaatg caaaaagtga
     6061 aatcagctgg actaaaaggc atgcaatttc ataatcaaag agagcgaaaa agtagaacga
     6121 atgatgatat tgaccatgag cgaacacgtg aaaattatga tttgaaaaat gataaaaata
     6181 ttgattacaa cgaacgtgtc aaagaaatta ttgaatcaca aaaaacaggt acaagaaaaa
     6241 cgaggaaaga tgctgttctt gtaaatgagt tgctagtaac atctgaccga gatttttttg
     6301 agcaactgga tccaggagaa taaaaacgat tttttgagga aagttataaa ttattttccg
     6361 aacgatatgg caagcaaaat attgcttatg caacagttca taatgatgag caaacccctc
     6421 acatgcattt aggtgttgtg cctatgcgtg atggaaaact gcaaggaaaa aatgtgttta
     6481 atcgtcaaga actgttatgg ctacaagata aattccccga gcatatgaaa aaacagggtt
     6541 ttgagttgaa gcgtggtgaa cgtggctctg accgtaaaca tattgagaca gctaaattta
     6601 aaaaacaaac tttggaaaaa gagattgatt ttctagaaaa aaatttagca gttaaaaaag
     6661 atgaatggac tgcttatagc gataaagtta aatcagattt agaagtacca gcgaaacgac
     6721 acatgaaaag tgttgaagtg ccaacgggtg aaaagtccat gtttggtttg ggaaaagaaa
     6781 taatgaaaac agaaaagaaa ccaaccaaaa atgttgttat atcggagcgt gattataaaa
     6841 acttagtgac tgctgcgaga gataacgata ggttaaaaca gcatgttaga aatctcatga
     6901 gtactgatat ggcgagagaa tataaaaaat taagtaaaga acatgggcaa gttaaagaaa
     6961 aatatagtgg tcttgtagag cgatttaatg aaaatgtaaa tgattataat gagttgcttg
     7021 aagaaaacaa gtctttaaag tctaaaataa gcgatttaaa gcgtgatgtg agtttaatct
     7081 atgaaagcac taaggaattc cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg
     7141 tttttaaggg gtttgtagac aaggtaaagg ataaaacagc acaattccaa gaaaaacacg
     7201 atttagaacc taaaaagaac gaatttgaac taactcataa ccgagaggta aaaaaagaac
     7261 gaagtcgaga tcagggaatg agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt
     7321 tttgatggtt ttgaacttgt tctttcttat cttgatacat atagaaataa cgtcattttt
     7381 attttagttg ctgaaaggtg cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa
     7441 acccttaaaa ttggttgcac agaaaaaccc catctgttaa agttataagt gaccaaacaa
     7501 ataactaaat agatgggggt ttcttttaat attatgtgtc ctaatagtag catttattca
     7561 gatgaaaaat caagggtttt agtggacaag acaaaaagtg gaaaagtgag accatggaga
     7621 gaaaagaaaa tcgctaatgt tgattacttt gaacttctgc atattcttga atttaaaaag
     7681 gctgaaagag taaaagattg tgctgaaata ttagagtata aacaaaatcg tgaaacaggc
     7741 gaaagaaagt tgtatcgagt gtggttttgt aaatccaggc tttgtccaat gtgcaactgg
     7801 aggagagcaa tgaaacatgg cattcagtca caaaaggttg ttgctgaagt tattaaacaa
     7861 aagccaacag ttcgttggtt gtttctcaca ttaacagtta aaaatgttta tgatggcgaa
     7921 gaattaaata aga
//
LOCUS       DANKHK010000101         7772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000101 DANKHK010000000
VERSION     DANKHK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(327..2708)
                     /locus_tag="PW109_002201"
     CDS             complement(327..2708)
                     /locus_tag="PW109_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2511998.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPTFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNVVTPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2698..3345)
                     /gene="recU"
                     /locus_tag="PW109_002202"
     CDS             complement(2698..3345)
                     /gene="recU"
                     /locus_tag="PW109_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2511999.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMHFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3409..3948
                     /locus_tag="PW109_002203"
     CDS             3409..3948
                     /locus_tag="PW109_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2512000.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELRQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4038..4457
                     /gene="gpsB"
                     /locus_tag="PW109_002204"
     CDS             4038..4457
                     /gene="gpsB"
                     /locus_tag="PW109_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2512001.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4479..4846
                     /gene="rnpB"
                     /locus_tag="PW109_002205"
     ncRNA           4479..4846
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PW109_002205"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5055..6221
                     /locus_tag="PW109_002206"
     CDS             5055..6221
                     /locus_tag="PW109_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2512002.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALVMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6267..7763
                     /locus_tag="PW109_002207"
     CDS             6267..7763
                     /locus_tag="PW109_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2512003.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
BASE COUNT         2438 a         1411 c         1635 g         2288 t
ORIGIN      
        1 gccttctttc aaaaaattat tcaaaaccat atattgtatt ttttatttga taataacaat
       61 gggattatta agaagtggca ctaaataaaa gactagtgtg tatttagccg atttaattat
      121 aaacaagtaa atggataaag tatatgtaat gataaaagaa aaatggattt tctaattttt
      181 aagctaaatg ttagatcaca catgtatatg ctggatagtc ttataaaaaa tcatgtaata
      241 cagcttggta catgattttt atttgtagct gttttttaca tagacacaaa aaagaaacca
      301 cgcttgtaga cgtggtttct tttttactat ctgtttccag aagctgaggt gctactggat
      361 ggtgtatggt catttgcagc atccgctccg ctagttgctg gctcactgga agaagcagga
      421 ggagcagatg aagacggtgt cgagctttct tcggatgaag aagaaggaac cgttgttgaa
      481 gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc agttgattca
      541 ctttgactag tagagctaga gctttgagtc gttgtctctg ttgttgagct tccaggagtc
      601 tggacatagc tggaagatgg aatagttgtt gatggtgtaa ctacattcga tctagctgta
      661 tattgatctt tgtagtacag ttcaccgccc acacgaatta atccgcttgg catctcccag
      721 tctgtatttg tcacgttagc tgaaacatac tgcataagct cgcgataaac atcagaggct
      781 acgtgtgacg attctgaagt gacaggtgtc atttttttat tgtaacctgt ccacacggaa
      841 atcgaataat ttggcgtata gcccgcaaat aggatatctg gatacacccc agaagaaata
      901 ccaagttttg catactcgtc atctgtgtaa ttagatgttc cggtcttacc ggcttgatat
      961 aacccagcga tttgcgcatt cgtaccagta ccttcagtga tcgtatcttt taaaatgtct
     1021 gtgatcatat aagctgtttc tggagacatg gctgtttttc catctggctc ataagtttct
     1081 tctgttccgt cttggaagac gattttatta acatattgag gtttgtaata agtccctcca
     1141 ttggcgaaag ccgcataggc tgctgccatt tttaaggaag aagctccgta ttttgtccca
     1201 tcttgttctt ccgtattact agagatcgca ttcgattgat ggatcgtact gtattcgatc
     1261 ccaagatttt ttaagaaaga tgccacttta tctgaaccga cttcattgaa taatttgact
     1321 gctggaacat tacgggataa gtaaagtgct tggcgtaaag taatggtgcc catatattga
     1381 ttatcccagt ttcctacagg agtcgaagtc ccttcatagt tatacggtgc gtcagtaata
     1441 gtttttccag tagaatattt caaatattca aaagttggtc cgtaatctgt gacaggtttc
     1501 atcgtagaac caaaatcacg ggaagtattc actgctaagt tgttgcctaa agttacgtct
     1561 tctgcgatgt gacgtccgcc aatctgggct tttactttcc cggtattggt gtcgataagg
     1621 gtggaggcaa cttgcatctc gtcgtctggg taagagacat actgatccgt gttgacgata
     1681 tcatatagtt tcttttgggc gtccagatcc aaattcgtat aaatctctaa accgtctgtg
     1741 tagacattct tatctgtttt ttcttgtact tcattgatga cttctttgac ataattatcg
     1801 acgatcttag tattgtcatc ggattgagtt agttcttgca gaccatccgt taccggtaca
     1861 ttaacagctt gatcatactc tgtttgagag attttttcgt tttgaagcat cgtatacaaa
     1921 acagtatctc gtcttttctt cgcctgatct ggataaacat aaggatcata ggcggaaggg
     1981 gcttgaggca tcccagcaag caatgctgtt tgcggtaaag aaagttctga taatttctta
     2041 ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc cgtttgacat atacacttta
     2101 tttacgtagt aggtcaagat ctcttgtttc gatttttttt gttccaatct tacagccatc
     2161 cacgcttctt gggccttgcg tttcagggtc tgatcctctg cactagtgga gaagaaggaa
     2221 agtttgatca attgctgtgt caaagtactt ccgccttgca gtccgccaga ggtaaagtta
     2281 gagagggcag aaccgatgat ccgaataggg tcaaccccga tgtgtttata aaaacggcga
     2341 tcttctaccg acacgatcgc atcttctaat gttttcggta attcattcgc agaaattttt
     2401 tcacgctttt ctgcgcctaa atcttcgaat agttcgccat cttgtgtata tagtttggaa
     2461 gatacggtag cgtctaattt tttatccgtc aattcgggcg catcttttgc gtaataccag
     2521 aaaagtccta cacctgctaa aaagaggata cagaaaaatg agagtattcc taaaaggatc
     2581 ttgataaata atcctttttt atgtgttcca gaggactttc cagaaccttt gccagaattc
     2641 ttcttcacac tctttttagg agtaggctgt ttctgtcgtc tagagcttct agtttgttca
     2701 tttgccattc ttgctttctc cttcaataga taatgttgat aaatagaagt caaccgcgtc
     2761 caaataagga atccgcggag aaataccagt tttgatctct attcctagtt cttcgatcaa
     2821 agataaaggt agtgatttgc gtcctttttc cttttgggcg tcccagtaag caatcaaatg
     2881 aacactattc aaataaaaac agcgcttcaa agacgaaaac cataacaaga caaagcagac
     2941 cccattttgt tcgaggcatg ctttcatatg ttcgatttga tgggcatgga agtttttaaa
     3001 gggaaaagat gttttgttct tggtttcttt tgcttcaaag tccaagtaaa agcctcgata
     3061 gacgccatta tagtctgtag ttgaggcttg ggtaaagtag gcttccttga tcacagcagc
     3121 acttctctta ggatagtcta ctttcacgat ttggataggg gtaggctttt tatggataac
     3181 cgccaacccg cgggaaagat aatatttatt actttcattg attgcttctt caaagtgcat
     3241 cccccgattg ccgaattcag tgatctgatt tcttttttct tgaatttttt cgacgggaga
     3301 ggcattctta taataaggtt ggccatttgg atagcgcaat accatcttta atcacactcc
     3361 taggccacat tatagcaaaa aagcggatag aaagaaaagg aatctgagat ggataagtca
     3421 aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat
     3481 cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac tccgtcagtt agcagaagaa
     3541 gggctggaat gggtactcgt tagtggaaat ctaggcgtcg aactatgggc tgtgcaaata
     3601 gtagcggaat tgaaagaaga atatccggaa ctatctcttg gtattttgta tcctttttct
     3661 gattttggta ataactggaa tgaagggaac caagaaaaaa aacaattagc agaacaatta
     3721 gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca gctaaagaat
     3781 cacactcgtt ttatacttga acatacagtg ggctgtctgc tgatctatga tgaagaattc
     3841 cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga tcatccctac
     3901 gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc gatattgtgt
     3961 ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt acgtaaatga
     4021 aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt ttacaacaag
     4081 aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc ttagacaata
     4141 tcatcaaaga ttatgaaact tacagcaaag aactccttgc tttacaagaa gaaaacgatc
     4201 gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa acacgcgtgc
     4261 aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta aaacgcttgt
     4321 caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt gcagtcaaac
     4381 cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat gataatgaaa
     4441 aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg
     4501 acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg
     4561 cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac aagctaaggc
     4621 ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg gggaaaccca
     4681 tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag gggcgctttt
     4741 tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg ttaccgcttt
     4801 gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc gcaaacatca
     4861 gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca tttgaaagct
     4921 atttttcaat acttcagatg agtcgttctt attacaaaac aaacttttgt atcaccggtt
     4981 attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt tttttataaa
     5041 taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact gcggcaagcg
     5101 ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc caagttgaga
     5161 acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat ttatggttac
     5221 gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg tttgatgaat
     5281 tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat gcagcttttc
     5341 ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc caagcgatca
     5401 caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct tctgtaccac
     5461 tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac catgtgatgg
     5521 taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg
     5581 gagcaccatt gaaagaaaac atggcagcag cacttgttat gttgacaaat tggcgaaaag
     5641 accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa gcagccttaa
     5701 taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct tgggattggt
     5761 tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca gattatgata
     5821 ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt gccaaagcca
     5881 atgcagaaga aatcggatta ggctcttcta ttacctttaa gcaacaagca gtcaaagact
     5941 tcaaaacgga taaagaatac ggggtcatcg tggctaaccc gccttacggg gaacgtctag
     6001 gagaagaaga aaccgttcga aaattatata aagaaatggg cgatgtgttc cgtccattaa
     6061 aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt tacggacaaa
     6121 aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta ttccagtatt
     6181 ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa ttgattcatt
     6241 caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa gaagtaaaag
     6301 aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa acaggtatgc
     6361 ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc atttattttt
     6421 caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa catccggaag
     6481 aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat gatttggaac
     6541 atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca gcacacggac
     6601 aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca ttgaatcaaa
     6661 atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag acgaattatg
     6721 atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat aaggtatttg
     6781 atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac
     6841 ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga tttgtagtga
     6901 aagtaatcga acaacttggt tatgatttta gccgtggtcg tttagatgac actgtccacc
     6961 catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg aacgaaacag
     7021 atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg tatgaacaag
     7081 acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg ggtatccatg
     7141 aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc tggaaaaagc
     7201 aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct tttgacaaat
     7261 tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca gacagtttga
     7321 cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc aatgaagggg
     7381 tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat ctgggaatac
     7441 gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt gcaagctttg
     7501 gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt cattctttgc
     7561 agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca atcaaaaact
     7621 ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa ttaatcatgg
     7681 atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa gatctgtatt
     7741 ttaatgtata tcaggtaaaa taaaatacaa tt
//
LOCUS       DANKHK010000102         7734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000102 DANKHK010000000
VERSION     DANKHK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..279
                     /locus_tag="PW109_002208"
     CDS             <1..279
                     /locus_tag="PW109_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512004.1"
                     /translation="SPTKLLSETDRLLLAEMIKEDFPSETISTENPELLQSFNRKVKN
                     GRVEIDSDNKKTYLIGKDYKKEFTWAADNQTRLKDSFGVEYSFTTDSK"
     gene            complement(637..1233)
                     /locus_tag="PW109_002209"
     CDS             complement(637..1233)
                     /locus_tag="PW109_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2512005.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKKLN"
     gene            complement(1258..1437)
                     /locus_tag="PW109_002210"
     CDS             complement(1258..1437)
                     /locus_tag="PW109_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512006.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(1514..2023)
                     /locus_tag="PW109_002211"
     CDS             complement(1514..2023)
                     /locus_tag="PW109_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512007.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(2119..2499)
                     /locus_tag="PW109_002212"
     CDS             complement(2119..2499)
                     /locus_tag="PW109_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2512008.1"
                     /translation="MNFTTIHHVAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSFGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            2831..3580
                     /locus_tag="PW109_002213"
     CDS             2831..3580
                     /locus_tag="PW109_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2512009.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(3765..5042)
                     /locus_tag="PW109_002214"
     CDS             complement(3765..5042)
                     /locus_tag="PW109_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512010.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLVATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQPKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(5121..5765)
                     /gene="pcp"
                     /locus_tag="PW109_002215"
     CDS             complement(5121..5765)
                     /gene="pcp"
                     /locus_tag="PW109_002215"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2512011.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(5964..6908)
                     /locus_tag="PW109_002216"
     CDS             complement(5964..6908)
                     /locus_tag="PW109_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2512012.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(6910..7596)
                     /locus_tag="PW109_002217"
     CDS             complement(6910..7596)
                     /locus_tag="PW109_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2512013.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYLGKGQ"
BASE COUNT         2280 a         1602 c         1299 g         2553 t
ORIGIN      
        1 tctccaacaa aattgttaag cgaaaccgat cgactgttac tggctgaaat gattaaagaa
       61 gactttcctt ccgaaactat ttctactgaa aatccagagt tactgcaaag tttcaacagg
      121 aaagtcaaaa atggaagagt ggaaatcgat tctgataata aaaaaactta tctaatcgga
      181 aaagattaca aaaaggaatt tacatgggca gctgataacc aaacaagatt gaaagattca
      241 ttcggagtcg aatatagttt tacaacggat agcaaatagg actgtgacaa aaatcgtgtc
      301 acagtccttt atttcgaata aacggtgttc aaaagccagt ttctcggtat taagccaaat
      361 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttaatactcg aaactaagcg
      421 actttttcac aacctctgat tcacggtgtt ccagaagtag cttcttcgga aataagccga
      481 aattttacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
      541 cacttctgtc acaaccttat taaaacctat tcaattttta gtaacgttca ttcttcttta
      601 caccattatt attttttcac tatttgacaa tttaaatcaa tttaatttct tttgcatatg
      661 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
      721 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
      781 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
      841 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
      901 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
      961 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     1021 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     1081 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     1141 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     1201 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     1261 ttttaaaaaa tcttcaatct tttcagtgat gaaataacca tgagtaacga ttcttgccgc
     1321 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     1381 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     1441 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     1501 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     1561 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     1621 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     1681 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     1741 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     1801 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     1861 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     1921 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     1981 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     2041 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     2101 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     2161 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatcccaaa
     2221 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     2281 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     2341 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     2401 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     2461 aacaatgatg gctacatgat ggatcgttgt aaagttcata tgagcactcc tattcttcgt
     2521 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     2581 agctgttttt tctttttata agaaaaaagg caaaaaaaaa acagaaagta ggtcctccaa
     2641 gacactcttc ctgttcccaa tataataaat gaaagtaaga aattatattt tcaagaaaac
     2701 aaacagaaaa ttatcataac acaaattatt tataattgaa agactagttt tttttcaaga
     2761 aaagcataca atagattgtg ttagtaaata cagaacacac tacatttagt gtcttaagga
     2821 gtcctcatac atgaagtcat attttcagtt tttagctcat cagctaaaag gctggccgca
     2881 acaaaattat tatcttttct ttttcagtct tggctgccag gtcatgactc ttgtcaatag
     2941 tcccatcact tggctgtccg ttattacatt tatcggtacc acgcttggtg tattgtgtat
     3001 tttatccatc aatgcggcaa aatcagtcaa cggtatttta ggtattttgt ctgcgatttg
     3061 ttttattatc gttggatttt cagcaaagaa ctatttaagt atcggtgaac agattgctta
     3121 tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa tggaatcata atatggcttc
     3181 aaaaatccga aaattcactg gaaaaagctg gtttatcgca atcatcagta ctttccttgt
     3241 ctatcttgtt tcaggattcc tgattggtcg ttatacagat gatcctcggc cgtgggtaga
     3301 tgccattgct ttttctatta gcttgactgc tggtatcatc agtttcttgc gttacaacaa
     3361 ccaatattac tggtggttag cttcagggct tgctcaaatg gtcctttggt atatttcatt
     3421 tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc tctgtttact tgctgaatga
     3481 tgtccttgca tttaccgttt caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa
     3541 aatggaagaa gaatactaca acgcacaaca acacgcataa aaaaactgag cgtgggacaa
     3601 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa taagaagcaa
     3661 atccttcgta aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt
     3721 cttatttctt ggagttaaac gcttctgtcc cagcctctat ttttttatag taattccttc
     3781 agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg ataatattcc
     3841 aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt gtcgattgtc
     3901 tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc ttttgagact
     3961 gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa tacttttaaa
     4021 aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa accatagcga
     4081 caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg gtttttcgca
     4141 aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt tctcaacacg
     4201 gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata tacgatggat
     4261 tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac atcctttact
     4321 aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt tggctgccca
     4381 ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc cgctatgtaa
     4441 atcggcattt ctacttggcg aaattctgcg agtcgctgag ctaattttat atccggtaaa
     4501 tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg ccatagtcct
     4561 tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc cagatcaact
     4621 tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag gtgatcggca
     4681 aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac gatgtattgc
     4741 acttttaatt ctctgccaca ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa
     4801 aacggattga tcttgaagtc agaaaacacc caatctgttg caactaaata atgttcttcc
     4861 ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg ctgttttttc
     4921 gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga aattttgatg
     4981 ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg ccattcttcc
     5041 acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac gttagcaacc
     5101 agccagtgtt tcttgctaaa ctagtgagtt gttccggcat ttccggaaat atcttcttta
     5161 ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc taaagacata
     5221 gaaggtgtac ccactggacg atcgatcact tgatcgggtg agaatggcac gtggatgaac
     5281 ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata catgatatcg
     5341 ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg atcttgtaca
     5401 ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc gttttcttct
     5461 aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc tgctagatta
     5521 attgcgacac gttcgaacga gactgctgac cgtccacctg cttgtccgac acagatcacg
     5581 acatctggct ggtgctgttg gatggcttct ttgactactt gactgctttt ttcgaatacg
     5641 gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat tttttttact
     5701 gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt tactagtact
     5761 ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta tcataccatt
     5821 ttccttgcac agcgtaaata aaggaaacaa tagtttaaat aacataacag atcaaactta
     5881 atatattctt ataaaaaaat agtgttccac tacaatatta gaagaggttg cgacaattat
     5941 tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat ttggaaaact
     6001 agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc tttcatctcc
     6061 agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag ggtcccacaa
     6121 aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg actaaatacg
     6181 aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt ccccatgatt
     6241 accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac atttccttct
     6301 ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcact tgtaaatacc
     6361 gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt gcccagcata
     6421 tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa catcaagatt
     6481 agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt ggtaaataga
     6541 gcaaacaaga tcgcaaagac accgatactc aaagccggaa taaagatttt cattcctagt
     6601 ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc tttgctgact
     6661 ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac gattgccgga
     6721 agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc tgacccataa
     6781 cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa tacgagtccc
     6841 atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg atttgtaaaa
     6901 aagtccatct tattgcccct ttcctagata atatttcttc gctaatttct tttctttaag
     6961 atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt ccactaaagc
     7021 aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga ctagcaacgc
     7081 ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt ccatagccga
     7141 agccatacct tttagttctt cttcatgctt aggatggact tctaatcctt ttgattcgat
     7201 ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc caccaaaact
     7261 cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa tcataccagc
     7321 agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac catttctttc
     7381 aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat tgtttacaaa
     7441 acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca aagctgtaac
     7501 aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc cgagaataac
     7561 aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat aaaatgacaa
     7621 tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc acgaaataga
     7681 cgagagtgta aaatattttg tgtaaatgaa aaaatccata caaaaaagga agtc
//
LOCUS       DANKHK010000103         7645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000103 DANKHK010000000
VERSION     DANKHK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            33..2360
                     /locus_tag="PW109_002218"
     CDS             33..2360
                     /locus_tag="PW109_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2512014.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2486..2815
                     /locus_tag="PW109_002219"
     CDS             2486..2815
                     /locus_tag="PW109_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2512015.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2855..3859
                     /locus_tag="PW109_002220"
     CDS             2855..3859
                     /locus_tag="PW109_002220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2512016.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3856..5285
                     /locus_tag="PW109_002221"
                     /pseudo
     CDS             3856..5285
                     /locus_tag="PW109_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5491..6663
                     /locus_tag="PW109_002222"
     CDS             5491..6663
                     /locus_tag="PW109_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2512017.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6825..7280)
                     /locus_tag="PW109_002223"
     CDS             complement(6825..7280)
                     /locus_tag="PW109_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512018.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2582 a         1441 c         1521 g         2101 t
ORIGIN      
        1 gattatttga tgatttttta ttgagaggag ggatgaaaat gattaagaaa actcaaaata
       61 aaatccaacg tatatgtcaa aaacattttg tttctagact caaagctgta tctaaaagaa
      121 aatacttaca tcccattatg atttcgatca tcttgatttt tactttctta gggagcttat
      181 caagtattta tgtaaacgtt tatacagaaa cgtcaaattt tgctaaccaa acgacagagg
      241 agacaaatag tccaccctat tctgaaatac agtccataga aagcaccaat cagtctagca
      301 acaacgagac aacctcaacc acatcctcga ctcaactgat tgatccgaca gaaaaagaaa
      361 tcacagcagc aaaagcagca ggggagaaag cttattcaga aacaggtaga agccagacat
      421 ttagtactgt tgcggccact gcgaatttca acggacatac cataccagat ggatatacag
      481 tgatatatgc caaagatgga gtcattgttg cggttaacgg atcgatgagt gcggataaaa
      541 ctgcgttaca aggcgtaacc aaagcaatcg cttttattga taaccgtcca gatatccata
      601 atctgtttat tggctccaca gtaaatggta ctactatcac attcccaaaa agtatctata
      661 tgggggtggc taccgctgga aatacgatcg attcagttac acccaatgcg gataaaagtt
      721 attcttttac accctcaaca tggggagggc ttcctgccgg aggaggtatg agtacttttt
      781 caatcggtat acagttagca aatgatattc cattcacggg gtactcgatc aaaaatgggc
      841 agatcgttga tggtaaggga aatgtttcaa attttccaac ctatcaagca gcaacggttc
      901 aggtcaatgc gaatcggaca ggaacttttt cttatacagt caatggtgga gcagcaaaaa
      961 tcggaacgta cgggactaca ttatctggaa tccatgtggg tgacaaagtg gttatcactc
     1021 caacagcagt aacgggctat tcttttgcac aaacaaacgg ttctttcaca gccctagaaa
     1081 gtaacgtcga tacggtgacc tatacaggca aacaaacacc aatcactatt aagtatcaag
     1141 actattttgg acagatgatt gctccttcta aaataatgaa tcttacttac ggaagtgctg
     1201 cacaagacct gacgactaat acgcctacta tcaacggata tacctttaca gctgtttcag
     1261 cgacagaaac aaagaaccaa tcagcaatga gtgttttagc tagtttagat acaaatggaa
     1321 atgtaatcgt aaaggacgca aatggaaaac agatttctga agtcatccta tattataaga
     1381 caaatgcaac agtatcaatc aatgcaaatg gcagtaagta ctatgatggt ctttctgttg
     1441 taccgatcgt taaatatgcc tttaatgata aaacagaatc aacatccctt ttaaacaatg
     1501 atctcagtat tgatcaaatc acatggaatc caactgattt caaagcgatg aatgaaaagg
     1561 gagttgaagt aactgcgcct actgaaattg gaacctatac gtcttggcaa ttgacaaaaa
     1621 gcggactagc taaactagct gccagaacaa actatttatt tactatcgtg cagacatctg
     1681 atgtctatac gattaagcaa atcagcggtg cggtagagct cagtggcagt aagacatatg
     1741 atggaaaagc aggtgtcccg tcaatccatg tcaagctagc agagggtgtc actagttcgc
     1801 ttactccagt acaagtggca cttagcagta tggattacac agcagatgca caatcagcaa
     1861 aaaatatggt taatgctggc agctatacaa ttaaactaac gaatagcgga atagacaaag
     1921 tcaaagcagc aaatcccaag ttgtcattta ctgatatagc caacacatca ggaacttata
     1981 ccatctataa agctaacgca gtgattactg tcgatgacgc tgcctttaat tatgatgccc
     2041 aaagtcactc gatttcggtt ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat
     2101 tggattatac cctaacagtt aacagccgga cagatattgg atcacaaata gtaacgatta
     2161 gtttaggcga aggaagtgta aacaataatt atacgattat cgcaacagat accgctaagt
     2221 tgacgatcaa tccgactctc ttgcttcctt cgacaggtgg gataggtcta ctgccctttg
     2281 tctttttagg actgatcttt attttttctg gcttctttta ttttatccat cgtaagaaaa
     2341 aagcaggtga acagcgatga aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct
     2401 cttttttatc ctactggtct tttttatagg ttctccgact gtgtcagcta gagaatcacc
     2461 tattacattt actttgcata atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca
     2521 aaacgacgga ataaatatcc cggataactt tcaaggagta aaagggttga acggcgttac
     2581 tttcgctata tatgatgtct cggatgaatt ttacaagctg cggtcagaag gaagtagtgt
     2641 ggaagacgca caaagaaaac tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa
     2701 tgtgacaaaa acagtagacg aggaagaggg cattgcttct tttagtgcat cggacaaaga
     2761 tgagcaaggt cgtgacgctg tttaccgatt tgcggaaata aagacttcag attaagtaaa
     2821 agaaaaatca gcaccatttg tcgttgtgct tcctgtgaca gattcatcta atcataaatt
     2881 gacgaccatc cacttgtatc caaagagcga acaaaaaata cctgctgcac tcacattaac
     2941 aaaaacagta gagaataagc agacagattt tgcagatggg gataaagtcc cttatctcat
     3001 caccgtaaca attcctgaga acatcaacga gataggaacc tatacgatca aagatacagc
     3061 tgatcctcag ttatggctag aaccagaaac gatcgatttt ttgattggtg gagacaagat
     3121 tcatacattt cacacacaaa aaacaaaaca tggttttgtc ttatcagatt ctggaaaaga
     3181 tcttagttcg tttgctggga aaaaattaac gatccgttac caaatgacat tgaaagaaaa
     3241 cagtgaaaaa acgacatttg ataacgatgt acgtttgacg actgacaatc aaacagtaga
     3301 aacacatctt gccatccaaa caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa
     3361 acaaaaacta gcagatgctc aatttctagt caaaaaaaat gaccaatact tagcgcaaga
     3421 aaacgggata aatcattggg tagccaaaga tgattcaaga gcaacgattt ttacttccag
     3481 taaagatgga acattttctg tccatggatt gtcttttgga agttacgaac tagtggagat
     3541 taaaccgcct aaaggatata tattgagtca atcaaccatt ctctttgaag ttgaaaaagg
     3601 cagttatgat ccgcttcatc ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc
     3661 gacgaataat ggaaaaacgc cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa
     3721 tggaggaagc tttccaaaaa caagcgaaga gatgcttgga ggtttctcaa ttttagggtt
     3781 gttgctagtc ctttcaacag ggacagcttg gttttataaa aaacaacaaa aaggaaacaa
     3841 taggagggaa ataaaatgaa aaacatcaaa acaaaaatat ggacgttttt aggaactgct
     3901 atcatgctgc ttcctttcgt tctcggccta ggaactgcag aggtttctag gccgtatcac
     3961 ctacaccaga aaatgttacg gttaatttgc ataaattgaa attcacttca gcaccagaaa
     4021 atcaaataaa caacggaacg gaattgacat ttccgaatag cgaaccgttg aacggcgttg
     4081 aattcaacgt atatgacatc actgctacgt actatccaag taaagacaca gcagttccag
     4141 cagacgctac acccttcgca agtgtaacga cttctggtga aggattagct aacctgactc
     4201 ttccaggtaa atctgatggg aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg
     4261 gtgtcgaaac gtcgccaaat atcgtgttaa gcttaccctt ttataacccg aattcatcag
     4321 accctgacaa acctttagac acgattcacc tatatgctaa aaatgtcatc aaagaaagca
     4381 atatcaaaat ccagaaagct gggaactttt caggggtaga taagttagca ggggcgcaat
     4441 tccagatcaa aaattcagag aatcaattcg tgaccggttt tgatgaaaac actggtgtcg
     4501 ctctctatga tcctgctgag tcagcgttga cattcacaac tgatactgaa ggatccatca
     4561 gtgtcaatgg attaatagac ggcaattata ctttagttga aacaaaagca ccagatggtt
     4621 atcaattaac caatccacaa aaagaaacta cttttatagt aaaaaacgga aaattgcaac
     4681 aaacaaccgg tatcatccat ggcgcagatg aagccaatct tgggaatcca aacggtgtag
     4741 tcaatcggga aaatacaact acggataaca cgattactgt agaaaatgtc caaaactacg
     4801 gtgatttcca cttcatcaaa caagatgtga acacgcaaga aaaattagcc gatgcagaat
     4861 tttctattct aagagcaaaa gatccaactg ttaaattaat gaaaaaaata gcaaatcttg
     4921 cctcaggaga atacccatat gcttggtcag atgaagtcac aggttctgac tgggaagaag
     4981 tgaaattgat ttctgattca gaagggaaat tttcaatcga aaggctgaaa caaggggcat
     5041 actgccttca agaaacaaaa gcaccagaag gctacgcatt acctaagaat gaagctgcgt
     5101 acactgaatt tacagtagat gatgatccaa caacttctga tgaaacaacg atcgataacc
     5161 aacctaaagg aatcttgcct tcaactggtg gtatggggat catcgtattc atcttggtag
     5221 gtactgcttt agttggagga gcagtgatct acttcaaaaa acgccatcaa gaaacagaag
     5281 cttaaactat tggaaacaaa aatcatacga taaataaacg atagtgattc aaatggggag
     5341 aaaggttgtg acaagtggtc ggtctcttgg tattcgctca acaaacggca aaataggagg
     5401 aactattttg ccgtttgttg agcgaatact caggtcaagg cgctgatttc acaacctctt
     5461 catatacata ttagaaaata ggtggacatg atgaataaaa gcagaataag aatactggac
     5521 atcttcatgg caatcatttt agtcgttggg atcgggatct tttcttatcc ttttgtagaa
     5581 gattcactga atgattttct tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg
     5641 aataagaatt cagaggaaat aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag
     5701 ttggcagagc aaaacgtatc accagggata ggcagtttca accaagcagt tgatgcaaaa
     5761 gcattaaaag atttacctag taatgccttt tttatggaac atatgcttgg agtcatcgaa
     5821 atcccaaaaa tcaatgtcag cttacctata ttcgatcaga caacggaaat atttttgcaa
     5881 aaaggaacgt cattattgga agaaagttct tatccgactg gcgggaaaaa cacccatgcc
     5941 gttctttctg ggcatcgtgg actcccagaa gcaaaattat ttacagattt gcctaagttg
     6001 aaaaaaggag accagttttt catccagatc aatggaaaga cattggcgta tcaagtagaa
     6061 aaaatccaag tggtcttacc tgatgaagta gattctttgg gtatccaaaa aggcagagat
     6121 ctcgtgactt tgcttacctg tactccttat atggtcaata cacatcgact gcttgtgacg
     6181 gggcatcgta ttccttatca agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa
     6241 tggaaaaaat ggaaattttt tatttggttt atcggtatac tacttggatc aatcggctta
     6301 gtttggctac tcatcgctta tttagattct ttggccatcg ctaagcgaaa ctatccttta
     6361 tctttttatg taaagaacac aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa
     6421 acgctcaacg gcaaacacta tattactaga gaaaaagttc catttgtgaa agcttctgat
     6481 gaatacggac tagtgagatt ttctgattta aaaggcagga actatcgctt gcaacatgag
     6541 gagctcctgc ttaaaatcca tgtaaaacat aaacattcga aacagttttc aatgaaattg
     6601 aaaaaaggaa gatataaact gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa
     6661 tgaatgaaga tgtccaaaaa agcagaggga acagactaaa tgaaaaaaga gagaagctga
     6721 cgaaatggac gtgtaaactg ctatttttgg tttactatct ggaacgccag cttctatttt
     6781 tttatgtttc agaagaattt tattattata gtataacttt tgaattagtt tccataacat
     6841 ttggagcaag gggttaaacc acgagcttga gcttcgctta aagtggcagg ggtatacgta
     6901 ccatttccac atttatgtgt atggtatttt gaaccggtcg gtgtgacata gacagtttgt
     6961 tcattatttt gctcaggagc tgcggcttgt gcctgctgag aggcctgttc agctgctgct
     7021 tgctgtcgcg cctgttctgc ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct
     7081 tgttgtcgtg caatttcttc ttgctttttc cgttcttctt ctgcttcttt tgctttgatt
     7141 gtggaatcaa ttgcagcaag acggcttgtc aactcttgtt tgttttcagg tagcgcttgg
     7201 atagctgctg aagcagttgc atagttttct tttgtaggat ttgcttcagc ttgtgacaaa
     7261 gcagattccg cttctgtcac ttttttttct agtgcagctt tttccgcttt ttgtttctct
     7321 tcttcttttt tcttttcaat ttgttcttgt tctttcgatg aatctaccgt actttcggag
     7381 atcgtggtac tgctgctgga agcctgagct gaacttgtgc tttgagtagt agacgtagta
     7441 gcaccacacc cactcaacaa acttcctgcg accaaaaaca atgcaactaa ttttttcact
     7501 aaatttcctc cttaagtaaa aaacaaccgt tttcaataaa tgtccttttc aattgtttac
     7561 ttacttgtta taataattgt gttatgaata gtgattgagg aaacttaatc taatcccgtt
     7621 ttgatacacg ggattttttg ctata
//
LOCUS       DANKHK010000104         7369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000104 DANKHK010000000
VERSION     DANKHK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            1..1590
                     /locus_tag="PW109_002224"
                     /pseudo
     CDS             1..1590
                     /locus_tag="PW109_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(1654..3825)
                     /gene="copB"
                     /locus_tag="PW109_002225"
     CDS             complement(1654..3825)
                     /gene="copB"
                     /locus_tag="PW109_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2512019.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(3845..6031)
                     /locus_tag="PW109_002226"
     CDS             complement(3845..6031)
                     /locus_tag="PW109_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2512020.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(6031..6240)
                     /gene="copZ"
                     /locus_tag="PW109_002227"
     CDS             complement(6031..6240)
                     /gene="copZ"
                     /locus_tag="PW109_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2512021.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(6253..6693)
                     /locus_tag="PW109_002228"
     CDS             complement(6253..6693)
                     /locus_tag="PW109_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2512022.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(6767..7306)
                     /locus_tag="PW109_002229"
     CDS             complement(6767..7306)
                     /locus_tag="PW109_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HDL2512023.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFIQQQQSFMETNHSWIIEGNYTSTLGKRIKEADKIIWLQVPRCRAIYRVILRSLK
                     RKINKKTRPDMPETFSERFDKEYLEFLSFIWHFEETHEPEIQQLIQENHAQNKLIILK
                     TRGEKQEFLTKLKIESKNN"
BASE COUNT         2026 a         1629 c         1308 g         2406 t
ORIGIN      
        1 caccaatctc tcgaggaaac gagtgaaccg atccgagaat atcttaatga tgaagggctt
       61 tatgacacac tccatgagta tcaattaacc ggaagtgcaa gaacaggctt cagtgagaat
      121 gatgaacagc ttggtggacc tttatatgat aatggcgaag ttgtttttac agcgaaccaa
      181 aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt acaccggttc tggatggata
      241 gaagatcagg acgcacccat ccaaccaatc caagaattcc ctgttgacat cacgagctac
      301 caaaccgtta tagaagacaa agaaaacatt acattgaccc taaaaaaaca agctttctta
      361 ccttatcctt atggaaatac taccttgact gatttttcat tagctagtgg acaaacgctt
      421 tactatttgc ctgaaagcca gcgattcatt cctgtcccga atgaatcgac acgttctgca
      481 actttacaaa cagaaaggtt agtttttacg agagaagaat taaaaagcag tcagttaaaa
      541 gctgatgatt tagaaaatca gcagacaaat cttcaattgc caaaaagttt gcctaaacgt
      601 attgaggact tggcggtcga attgaccaaa aatgaagcaa ctttaattcg taaagtcgaa
      661 gctgtctaac agtatttaaa aacaagcgga aatttccgct attccaaaac ggataccggg
      721 catactccga aagaacacga ttatgtggat tatttcctct ttgattcaaa agtcggctat
      781 tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa cacttggtat cccagctcgc
      841 tggacaaaag gttttacaaa tgggctcaga acaggcacag ataatcaagg gaatgaggaa
      901 tacagcattc tcaatagtca tgctcattct tggccagagg tatactttcc tggatttggc
      961 tggcttccat ttgaaccaac cccttcattt gccaatccaa tgggttcaca accagttgct
     1021 gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa atgaaagcga aaatatggca
     1081 gaaactattt ccagtgagag atctgaggtc attccatcag aagaaacaac actcacacca
     1141 gcttataaaa tcgtcttatt cagccttgga gtgtgtttat ccgtcttgtt gttctttcta
     1201 ttgttattca gaaaatctct gcgctggaca agttatctta tctatcttca tttttttgct
     1261 tctactaata cgattgcata tcaaaaactt cttaaccctt tggaaacatt ggtaccgcgc
     1321 aaacccgcag aatctttgca ggaatatagc ctgcgaatag aacaagaatt caagctagat
     1381 ctgcgtttca ttcgattaac tactcaatat gaagcagaaa tatatggacg acaagcagat
     1441 ccggtaaaat tcgataaaaa aacctattta gaaataacgc atataataag aaaaacggta
     1501 tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaac aagataaaat ttctggagaa
     1561 tctacttctc gaatgccgag aaccagctga ttgagtatcg attactcgaa aaaaaggttg
     1621 tgacacgttt tttgtcacaa ccttttttca ttattatttg attttcaatg tcaaagcatt
     1681 tagagctaca actaccgtac ttaacgacat caagacagca ccaactgctg gacttaatat
     1741 gataccaatt ggcgcaagaa ttccggctgc tagcggaatt gcaagaatat tgtaacctgc
     1801 cccccaccag aggttctgga tcattttctt tcgcgtttgc ttggctagat caagaaaatg
     1861 caaaatatct tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga ttgcgatatc
     1921 tgtccctgca ccgattgcca tcccgattga agcacgggct aaacttggcg catcattgat
     1981 cccatcaccc accatgatta cttttttccc ttggtcaagg tatttctgga caacagcttc
     2041 tttatcatct ggtaataaac cgccataata ttcttcgatc cctaaataat ctgctactgc
     2101 ttgggcagcg tttttattgt caccagtaag catgacagga gtgatttttc tttctttcaa
     2161 tgttcggata aattcttttg cttctgtttt tactacgtca cctaaagcaa cgattgcagc
     2221 tagatgattt tctaaaatca ggaagctgat ggtatttcct tgttcttggt aaggtttcaa
     2281 taatgcttgt tctacgttta atccgagacg ctcaacttct ttttcattaa ctattttcac
     2341 ttgttgtttt ttgactgttg cctctagccc gactcctgaa agatttttta gattttcagc
     2401 ttgataaggc ttgatttcat gttctttcaa ataattcata atgccaacag ccaatggatg
     2461 attggcattc gcttccaagg ctcctagata ttgaaggatt tcatttttat tgaacgcatc
     2521 atcaagtact tcgacacccg ttacagtaaa ttttccttct gttagtgttc ctgttttgtc
     2581 cagcatgatc acatctagat cattggcttg ctccagtgca ttgcggtttt tcaaaagtaa
     2641 tccgttttta gctgctattg atgtggagcg agcaacaact aatgggatag ctaaacctag
     2701 agcatgagga caggcgatga taaagactgt taccatacgt tctaatgcat taggaagatc
     2761 tgtcaagaac aaccaggcga tgaatgccag gatacccaca attaatgcga cataaaacag
     2821 ccattttgcc actttgtcag ataatgactc tagcttggat ttttctcctt gtgcttttcg
     2881 caccatttcc ataaccttcg ctagataacc attttcacca gttccagtta cttcgatttc
     2941 gatcgttcca tccccattga tagaaccgcc aatcactttg tcattggctt gttttttgat
     3001 tcctcttgat tcgccagtca cagctgattc gtcaacaatt gtttcacctt tcaagattac
     3061 accatctgtt ggcattttat caccagaccg gacgatcaaa cgatcacctt cgtttacttc
     3121 tttcaatgaa actgtctctt cttttccatc tttcgtcaag cgtttcactg attctggcag
     3181 taattctgct aatttttgta aagcattgct ggcattcgac acagcattca tttcaatcca
     3241 gtgacctagt agcataataa cgatcaaagt agccaattcc cagaagaaat ccatgacgtg
     3301 tgtatgtgga ttgagcagat ttgcgataaa cgaatagaca ctataaatat atgcaactgt
     3361 aatccccatc gcgatcagtg tcatcatagc aggacttttt tgtttcaatt ccatcttagc
     3421 tccacttaaa aatggctgac cgccatagat aaatagtata gttgctaaga tcaatacgag
     3481 ccaatcagaa ccaggaaacg ttacttgaaa tggaaattcc attcccatca ttggtgaaaa
     3541 aagaatgatt gggatagcta ggataagcga tagccaaaat ttttgtttga aattccccat
     3601 gtgcatggaa tgatccatgc cgccgtgttc gtgatccatg tgcatcgaat ggtccatttg
     3661 tttgctgttt gctatatctc catggttatg tcccatgtgc atggaatgat ccatctgact
     3721 atggtcatgt ttcatctcca tctgtttttc atgagattct gtactttctt tgtttactac
     3781 ttctttttta tgctcatgtt ttttctccat atcgttccca ttcattttta ctccatccct
     3841 tctcttactt tttcatgtgt cgatttaagc tcagggagtt caacaataca ctgacggaac
     3901 taaacgccat cgctcctcct gcaatgattg gattcaaaaa gcctagtgcg gcaaaaggaa
     3961 taccaatcgt attatagaca aaagcccaga aaagattttg tttgattttt ctcaatgtca
     4021 acctagacaa ctcgattgtt tgtgcaatgg atgccaaact gctattcatc agcgtcacat
     4081 cggctgtttc catcgcaatg tccgttccac ttcccattgc cataccgaca tcagctaatg
     4141 ctaaggccgg tgcatcattg attccatcgc ctgccatacc gacttttttg ccatctttct
     4201 gaagtttttc tacgtaggct gctttatcct ctggaagtac ttcagcaaag atatgctttg
     4261 gatcaatacc aacttgttta ccaatcgttt cggctgccag tttgttgtct cctgtcagca
     4321 taaaaacatc taatccttta ttctggagct gcttgattgc ttgtttggct tctagtttga
     4381 tttgatcagc cacggcaatc aacccgatca cttgcttttc atcagctaaa aacatgactg
     4441 tttttccctg ctgttctaat tctaacgcgt gttcttggta ttcatcaaat gataggttca
     4501 attcaattaa tcgtttacgt gtacccgcaa aatagcgtac tccattaatc gtaccgctga
     4561 tcccagcacc tgggtgggct gtaaaatcaa tcatatcgta tgttgctgct tcttgcagtt
     4621 ttccgtattc cacgattgct ttacctagtg gatgttcaga agcttgttct aaagtataaa
     4681 aaatagatag gacttcttta gaaccgacta gatctgtaac tttgggcttt ccttctgtaa
     4741 tcgttccagt tttatccaaa acaatgctgt ctaagtgagc agctgcttct aatgcttctc
     4801 cgcctttaat taaaattcca tttcgtgcac caacacccgt tcctaccata atagctgttg
     4861 gagtcgctaa acctaatgca catggacacg caatgactaa tacagagaca ctatggatca
     4921 atgcgagttg ccaatcacca gtgatcaaac ctgtagctat gagcgtgata aacgcgatga
     4981 ctaatacgat cggaacaaaa atccctgaga tccgatctgc gattttttgg attggtgctt
     5041 tgctcccttg agcatcttcc accatttgaa tgatttgcgc taatactgtc tgcttgccga
     5101 cttgtgtgac ttccatgtgt agcagaccat tcgtattgat tgtcccgcca aaaagcgcgt
     5161 catccggatt cttttctaca ggaagatttt caccagtaag catactttca tctatcgcac
     5221 tgcttcctga aataattcga ccatctgctg gaatctgctc accaggacgg atgactagtt
     5281 gatcaccaac gactacttct tcaatcgcta acgtttcttc tttcccgttt cttatcactt
     5341 gggcggtttt tgtttgaaga gacatcaatt gtttgattgc attacctgtt ttcgtttttg
     5401 ctgtatgttc caaatatttg ccgagcaaaa tcagcgtgat gatcatactg ctgctttcaa
     5461 aataaagatc ttgggggtgt ccacggaaaa atccgttgta aatgctcaat gcaaaggcag
     5521 ccgatgttcc aattgctacc aatacatcca tatttggtgc tttcgtcttg attgcatggt
     5581 acgcaccttt ataaaaacga gcaccgacat aaaattggac aggagcagac agcactaact
     5641 ggacgattgg aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac atcatagcaa
     5701 tcattgacaa catcaacggc aaagtcaaaa ccgtactcaa tataagatcg aatagcattt
     5761 ttttcaaata cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca tcgtacaaaa
     5821 ttgcaccata gccgatgttt tctacgcttt ggattaattt ttcagtggtc gtcccttcat
     5881 atttgacact tgctttttca gtggccaagt tgactgtggc attgaccaca ccaggttgtc
     5941 cgttcagctc tttttcgatt ctagcagaac aatttgcaca agtcatacct gtaatgacga
     6001 acgtatccat tttggtttct gctgttgcca tcaaatcacc tctgctttat aacccaatcc
     6061 attgattgct tgacaaatct tttcggattg aacttttgtt tcatcaaact tgaccgtacc
     6121 gtttgctttt ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc ttactgtttc
     6181 ttctactctt gctgcacaat gattgcagct cataccttcg attgaaaatt gttgtttcat
     6241 tttttcttcc tcctattgat gttgtttaca ttcacattga cctgggatac agttacattc
     6301 gatcgtatct actggttgct tttgttccag ttgtttcaaa atctcattga tatcttcttg
     6361 agtcaaagtg gcttcttcta ctagatccgc aatcgtctct ccaatccttt ttgcacagat
     6421 atgggaaaaa agattttccg tcgcactgcg aactgtttcc atctccgaga cagctggatg
     6481 ataaatgaat ttttttcctt cttgttctgt ccagatcact tcttttttta ccaaacgtcc
     6541 cagcaaagtt ttgatcgttg ctggtttcca atccatcgaa tcagctaaga tctgcgtgat
     6601 ttcttgggct gtcgtctgtc caagtgtcca aataacacgc atgatctccc actctgaatc
     6661 actgatcttt actggcgttt tgactttttc catttatttc acacctttcg tttacaaatg
     6721 taatcaataa cttatggata cagattacac ttgtaaacaa aaactgtcaa ttatttttgc
     6781 tctctatttt caatttagtt aaaaattctt gtttttcccc tcttgtttta aggataatca
     6841 gtttgttttg tgcatggttt tcttggatca gctgctgtat ttcgggttca tgggtttctt
     6901 caaaatgcca gatgaaagac agaaattcca gatattcctt atcgaatctt tctgaaaatg
     6961 tctcaggcat atctggtcgg gtcttcttgt ttatctttct tttgagggaa cgtaaaatca
     7021 ctcgataaat cgcccggcaa cgcggaactt gtagccaaat gatcttatct gcttctttaa
     7081 tcctctttcc tagtgtagaa gtatagtttc cttcaatgat ccatgaatga ttagtctcca
     7141 taaatgattg ctgttgttgt atgaaccact tttttgcttg gatgctgtaa tctgtcgtat
     7201 gccataaaag gtctagcggc agtagcggaa agccggtgat ttctttgatc ttccttgcga
     7261 gggtcgattt tcccgaccca ctttgtccaa tcacgatgat cttcattctt ctccctcact
     7321 tctcttatca ttttgatttg atcataccac ttataacgcc gcaaaaaaa
//
LOCUS       DANKHK010000105         7173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000105 DANKHK010000000
VERSION     DANKHK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            311..1168
                     /locus_tag="PW109_002230"
     CDS             311..1168
                     /locus_tag="PW109_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2512024.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENSDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1161..2063
                     /locus_tag="PW109_002231"
     CDS             1161..2063
                     /locus_tag="PW109_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2512025.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMEISAKSTRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2095..2445
                     /locus_tag="PW109_002232"
     CDS             2095..2445
                     /locus_tag="PW109_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2512026.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKCAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2494..3417
                     /locus_tag="PW109_002233"
     CDS             2494..3417
                     /locus_tag="PW109_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2512027.1"
                     /translation="MDEEKNVTETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3495..4388
                     /locus_tag="PW109_002234"
     CDS             3495..4388
                     /locus_tag="PW109_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2512028.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGC
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4426..4989
                     /gene="amaP"
                     /locus_tag="PW109_002235"
     CDS             4426..4989
                     /gene="amaP"
                     /locus_tag="PW109_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2512029.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            5003..5191
                     /locus_tag="PW109_002236"
     CDS             5003..5191
                     /locus_tag="PW109_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2512030.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5204..6106
                     /locus_tag="PW109_002237"
     CDS             5204..6106
                     /locus_tag="PW109_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2512031.1"
                     /translation="MNNNNKNNKNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPRHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEIIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6138..6380
                     /locus_tag="PW109_002238"
     CDS             6138..6380
                     /locus_tag="PW109_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2512032.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6649..7019)
                     /locus_tag="PW109_002239"
                     /pseudo
     CDS             complement(6649..7019)
                     /locus_tag="PW109_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2615 a         1216 c         1412 g         1930 t
ORIGIN      
        1 catatctaat ttcctgttac cttaagatta aatgagaatt taaaaaaatg aaactaactt
       61 gctttgtcat tatgaatttt ttcaaaaaac attcttaaga aaaaggtggc tatatatagt
      121 agtttttggt caataccttt gaataaaaag ccatgaaaaa cgaatagtta gcgcttacca
      181 atgcgttttg tgataaaaaa attttaaaat attcaatgaa aacaaagcat atattttgct
      241 ttaaaaccaa atgaggaata ttctagatgt ataacaggtt agaaaaagat tagaaaaagg
      301 agtgattttg atggagaaga gatacggcgt agttcctgtg gtaactgctt cagacgaaaa
      361 ttatgctcct tacttgagcg tgatgatcgc aactgcatta gaaaattgca ataaaacaag
      421 acgcataaaa ttttatgtga ttgacgatgg tctatcagaa tatagtaaag aaggattgga
      481 agaaacggta aataaatatt caagtaatgc aagtatccaa ttcctgacag tggaaaaaga
      541 tatctacgaa gacttcctag tcagtgacca tatcacaact accgcttact taagaatttc
      601 tttaccaaac ttattagcta aagaggatta taagaaagtc ctttacttag attcagatgt
      661 gttagtacta gacgatatcg taaaactata tgatgagccg ttaaatggaa aaacaatcgg
      721 cgctattatc gatccaggcc aagtgaaagc tttggaaaga ttaggaatcg attccgatga
      781 cttgtacttc aattcaggag tgatggtgat tgacattgat caatggaata aaaaagaaat
      841 tacagaaaaa actatccatt acctcagtga aaatagcgat cgaatcatct atcacgacca
      901 agatgctttg aatgctgtac tttatgaaga ttgggaacaa cttcatccaa aatggaacat
      961 gcagacttcg ctgatttttg aacgtcatcc agcaccaaat gaaaaatatg aacgtcagta
     1021 taaagaagga aatgaaaaac cttcgatcgt tcactttaca ggacatgata aaccttggaa
     1081 tacgttgaaa gatcatccgt ataccaattt atacttaaaa aaattagcac acagtactct
     1141 aacgaaagta ggcgagatca atgaataaaa aagaaatagc agtagtcgca agttgtaaca
     1201 caaaattcgt accacattta gctgcattat ttgtctcagt attagataat tgcaacccgt
     1261 ctaaatttgt tcggttctat gtgattgacg atgatatcga ttttgaaagt aaacagcttt
     1321 tacgcttctc agtgaaaaac gcacgaatga actcagatgt ggaattcttg aaaatcaata
     1381 aagaattctt tacaaatgtg gtaatcagcg atcgtattcc ggaaacagct tattatcgta
     1441 ttgcgattcc ggaattgttc cgcggcacag aagttgaacg tatcttatat atggattgcg
     1501 acatgatcgc tttacaagat atctctaaat tatggcgtct tgacttcggt gattcaatcg
     1561 tagcagctgt tgaagacgct gggttccatc aacgactgga aaaaatggaa atttcagcta
     1621 aatcaacgcg ttatttcaac tcaggtttga tgctgatcaa tgtgaaaaaa tggttagatg
     1681 aaaatattac gcaaaaagta ttagatttca tcgaacataa tccagaaaaa ttacgattcc
     1741 atgaccaaga tgcattaaat gctatcttac atgaccgttg gttaccactt catccgcgtt
     1801 ggaatgcaca aggctatatc atggcaaaag caaaaaaaca tccaacagca gcaggcgaac
     1861 gtgaatacga agaaactcgc aacaatcctt acattatcca tttttcagga catgtgaaac
     1921 cgtggagcaa ggattttgaa gggccaacga agaaatatta cgaaaaatat gcagggatga
     1981 cggctttccg ctgtgtagct aaattcccta aatatccgaa atacgcaaaa gttcaaccaa
     2041 aaacagatgt agttgttaaa taatagaaga caacaactga agaggggagt aggaatggaa
     2101 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     2161 atcgcactcg cgctcatcaa tgattccgat ccatttttaa gggacaaatg tgcttttgca
     2221 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     2281 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     2341 cgattgaaca tggactttaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     2401 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     2461 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaacagaa
     2521 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     2581 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     2641 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     2701 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     2761 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     2821 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     2881 ctagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     2941 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     3001 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     3061 ttatggaatt ctgaaacttt catcaaacgt ttagatgatg aacagctact ctatgatgtc
     3121 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     3181 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     3241 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     3301 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     3361 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     3421 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaac ttaaaaacta
     3481 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     3541 agaaatttcc taaacaagat caggatacac ccgctttaca gcagaatatg attcctgttc
     3601 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     3661 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     3721 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     3781 acgtgaaaga tgcaggatgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     3841 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     3901 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     3961 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     4021 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     4081 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     4141 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     4201 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     4261 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     4321 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     4381 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     4441 aaaacgatcg gagtgatcgg aacgttactt ttgctatctg tgcttttgtt tgcgatacta
     4501 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     4561 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     4621 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     4681 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     4741 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     4801 gccgtcaatg taaaaggaga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     4861 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     4921 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     4981 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     5041 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     5101 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     5161 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     5221 aaataataag aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     5281 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     5341 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     5401 aaagcatgca cctcgtcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     5461 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     5521 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     5581 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     5641 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     5701 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     5761 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     5821 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaaataa tcggcaaaga
     5881 agtaaaaaat atgacacact tagaagtgat cgaagttaac gctaacgtag tggacatcaa
     5941 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     6001 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     6061 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     6121 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     6181 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     6241 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     6301 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     6361 tctttgctcg aaaactgtaa aaagactttt aaaagaggtc gtgaaaaagc tgttcttcat
     6421 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggctta
     6481 ttatcgaaga tgcaagcttt tgaacactgt ttattcggaa taagaggctg tgacaagact
     6541 tttgtcacag cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc
     6601 ttaaatgtgt ccttgttgat tgctataaga cagctttttc acaacctttt aataaaacaa
     6661 tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata cgaccacttg
     6721 gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa agcaatggat
     6781 cagaaactca aagagattga aaaagacaag gaagaaatat atctcttttt tactcttgtc
     6841 tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag taaacaaagt
     6901 ttcgtaagag ccgtaatcgc atcatgttgc tctgctagac gattgacgag caggaaaaat
     6961 gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat aaatggcata
     7021 ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt tgtttgtgaa
     7081 gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa tcattattat
     7141 caaaataatg ttaaaaataa aaaagaatag tcc
//
LOCUS       DANKHK010000106         7069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000106 DANKHK010000000
VERSION     DANKHK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..546)
                     /locus_tag="PW109_002240"
     CDS             complement(<1..546)
                     /locus_tag="PW109_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2512033.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIR"
     gene            complement(561..1604)
                     /locus_tag="PW109_002241"
     CDS             complement(561..1604)
                     /locus_tag="PW109_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512034.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1737..2141)
                     /locus_tag="PW109_002242"
     CDS             complement(1737..2141)
                     /locus_tag="PW109_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2512035.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2374..3009)
                     /locus_tag="PW109_002243"
     CDS             complement(2374..3009)
                     /locus_tag="PW109_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2512036.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3275..4327)
                     /locus_tag="PW109_002244"
     CDS             complement(3275..4327)
                     /locus_tag="PW109_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2512037.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4340..5278)
                     /locus_tag="PW109_002245"
     CDS             complement(4340..5278)
                     /locus_tag="PW109_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2512038.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHIQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5729..6379)
                     /locus_tag="PW109_002246"
     CDS             complement(5729..6379)
                     /locus_tag="PW109_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512039.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6479..6988)
                     /locus_tag="PW109_002247"
     CDS             complement(6479..6988)
                     /locus_tag="PW109_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2512040.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT         2189 a         1491 c         1085 g         2304 t
ORIGIN      
        1 acgaatagat cttcttatca tttctatttt agtagtcatg cgttgttgtt cttctaaagg
       61 tggtaatggt ataagtagtt tagataaatt tgtaccattt acatttggtt gcccagttcc
      121 gtaagaaatt ttttctaata acttccaata taatggcgag tctaagaagc agtctacgta
      181 ttctgataat aatttctcaa ctaacctaat tcgaatcaag taagaagcaa aaacagattc
      241 ctcacttatt tccttaacca agaaactttt tcccatagtt cctcctgtcc ttgctatcaa
      301 aatgtcattt tcttccaatc ttaaatcaac tagtttagaa ttactaatat cacagtaagg
      361 cacactggac caatttacac gtccctcttg tatatctgtg attctcaaaa acttaacatt
      421 gccttgtttt ttcgccgaat ctgtatagcc atattgaatc ttagtagaaa tacttcctaa
      481 ataaacccat agtcgaacat cgctagatga tttttcttgt cgttctttat gaacaacagt
      541 gatcatcttg agttgtccga ctatttttta gttgcagcaa tctttttggg acgcttattt
      601 gcatgaagca acgttgctaa gtctagtaat aaaacattgg ctaacacagg attggctttc
      661 tcgcgatacg ctgcggcgcc actttcaaac aatactgcta gacgatcttc tacaacaacg
      721 atttgttcca aatacggtgg aaatttgatt cgttttagga tccggctagg agagtcccgc
      781 tcggacacta agagttccga ggtataagga ccataagaac gactggctaa gagataatgg
      841 ccataaaaag ccacaccttg aatcttatca gatgtttttc tgctcattgt cacggttggt
      901 tggattttgt ctttgcggcg aattgctgtt ttcatttcag ctatcgtcgg caatagatag
      961 aaagtcaact gtccttcacc ttttagtgtg aagttgccag ataccagttg atcattgttt
     1021 tgagtcaaat acgatgcttg tggaatctct gagagatcag ttgtcagcca ataagcgatt
     1081 ggctgtcccg tctcttcaga tgtatctgct aagatatcga tcaaacgtaa agcggctact
     1141 cgtccagtag cagatcctgt gattgtcaac cataaaagtt cccgttttgt atcatatact
     1201 aagcccccta catgtggccg tttaggtaaa atcagcagtt tgatcggctg tcctgtttct
     1261 ctatccagta catgaataat agaatgatgc tgatggtcat agcagtaggc agaaataaac
     1321 acaaaatctt gagaaaaagc caacccttgc ggcgtcatcc attgacaaac ctcattttcg
     1381 ccggtttccg gcaccaccat tcttgtttct gttaatccag gaatgttata ttgttttgct
     1441 tcagcctttt cccaagctgc ttgaacaata ggataccgtt ttaacttttt ttcaatatcc
     1501 tgatgtatcc gttgctgctt ccaatgctta aactttagga catagtaaac aagaaggcca
     1561 atgaccagca acactaaaat cattattgct acccgctgtg tcattttttt gcctccttgt
     1621 tctattcgat caatcgtcca tataccgcta ttaattaccg taactacatg tttcttttac
     1681 tagcgtttct ttataggatc tttcataatc tcatttatag taacattaag aatcccttat
     1741 tctactatag tcacttccac atcaatattg cctcttgttg ctttagagta tgggcaaaat
     1801 gcgtgggctt cttctgcgat tttctctgct tggtccttct caagtcctgg caaagaaact
     1861 tctaaaacga ccccgatttg gtagccccca tcttctaatt gaaataaaga tacccgtgct
     1921 ttgacttcag aatcagactc aagattattt tcttccagca tgtgttgaac cgcgccgttg
     1981 aagcaagatg cgtatccagc cgcaaaaagt tgttccggat ttgtcccttc actatggatt
     2041 cctgggcggt cgatcttcat gtgcatactt ccgtttggtg cctcaacttc gccatcacgg
     2101 cctccacgat tgatcattgc tgtttcgtac atttttttca ttgagatcat cctttctttc
     2161 gtcgttctat attaagtgta acgagtattt tttaatctga caaaggtaat gcttgcattc
     2221 ggcgacatca gttagacaac atcgatgatc agtgatagaa gcagtttatc tatacaaaaa
     2281 aagaagggtt ctatgatatt tggtgtcctt actcgccttg agtaacaaca ccaaatatca
     2341 tagaaccact taatcagctt cacactgctt tttctacttt cgtagttgaa aaaaacttaa
     2401 acaaatcatc ggcaaatagt taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt
     2461 aaagaaggca aactgactaa tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag
     2521 taaactaata actgttaaaa caagataaaa atggctaagc gtaccttgac cactttgacg
     2581 ataaagcaga acaagtaaca atgggaaaag tagaaagcct gcatagccta gcccagaacc
     2641 tatattatgg atccaagtcg atagattaca agagctttca ttcgtattga tactgaataa
     2701 gccagtaaaa atacaatcac ctatcccata taaaccaact gttgcaacaa caagtattgc
     2761 aagtgttttt gatgttccaa caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa
     2821 aagtccagat acaactgacc agaccaagaa agcacgtctg actggactat ctacgtcacc
     2881 aaacacacta attacggtag tcatttgatt cagtttggga taaaaaaggc caaggatata
     2941 cggtgtcgat aaatcactca tcacacctaa taataaaaaa taaaaaccat atttttgcaa
     3001 aaaaatcatt ttcttcccct actttttagt gattattcga tacatccaac aatcaaattt
     3061 tttctcattt taacacatat ttttattcta gttgtctttt gaacaatcaa taacataaag
     3121 gtgagtttct tctacatttt aaaaaatcga atggcactga agagttcagt tgtcagcatc
     3181 aatctcctac tcaaaaaggc aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg
     3241 catccattga ttgaaaattt cttagtaaaa tctactagcc taaaaatttg aagacttctt
     3301 ctcgatcata cagtttaaat actttctcgc ataaaaattg agtaattagt gccgcaaaaa
     3361 cagggatgac taaccaacaa agaacaacaa gaaagccatt tagccctgca tctaaagaag
     3421 ccaacggacc aacaagtccg accacgccga aaccagcaga agcaggcgta cctgaaatag
     3481 aaaatagagc gacaggaata gctgtaatca ctgcactaac taaacaagga ataagaatca
     3541 caggttttct aaatagatta ggcatcatca tcttcatagc gcctaaagca atcgcaattg
     3601 tcacacctga cttattaacc ttccaagagt tcactactaa aacaaaagtg gttgcagcta
     3661 cgcccatcgc agccgcacca gcagaaacac catctagttg gattgccatt ccgattgcta
     3721 ctgttgaaat aggagagata atcaaaaagg caaaggaaca accaatcaag atagacatta
     3781 acagcggttg taaggtagtg aagttattaa tcaatgatcc aattgcagaa gtaattgctg
     3841 ttacaaaagg gaataaataa aaaccaagta aacctgcacc tacacctaca acaatcggtg
     3901 ttaacacaat cgacactgac ccaaaactat ttccaatcag taaaatcagc ccgacagcaa
     3961 tcgatgctgt cagcatcgta ttaataatat cgcctgtacc agcaccaacg taaacacccg
     4021 tttcacccaa atcaggtgtg aatttgacta caccagaacc aacaaaagaa gctccggcta
     4081 caaccatcat tctcatcgga tcaaatccaa attgtaaggc aatcaaacca ccaatgatca
     4141 aaggtgttgc tagttgaaaa ataactgcta actgtgcgat cgttaccgca aagggatacg
     4201 cactaaaata ttttaaaatg gcacctaata ctgcattagg aatcaatcca ataatgattc
     4261 ctgttgctgt accagcgagg actttattca gaaaaatttt cggtgatatt ttttgtgaag
     4321 tttccattca atctctcctt tattttacgt tcaaactatc ttcttttgca tggacaagct
     4381 cagtcaagaa atcacaataa ggcgtcgcaa caccatattt cttccctttt ctagaaattg
     4441 cgccattgat ataatcgatt tctgttaatc gatggttctt aatcaagtct tgatacattg
     4501 aaggataatg taacccaatt gtttccgggt caaaacaaga ttcacaatgt gcaatgactt
     4561 ctgggacatc tagttcaatc ttctctactg ctgctacttt ggcaaactca ttaacaatcg
     4621 tcgccaccat tttatgagca gtcgatgttt ttcctagctc agccatatta acgtctaaga
     4681 ttgtgcatag tccgttcatt gttccgttca cacatgcttt gcgataaata gaatagtgaa
     4741 tattgtcaga aaaatgagca tttaatcctg attcagacaa tttgtctgct aatttttttg
     4801 cagctgcttc ttttccatca cctaaatttt gtaattctac tgaaccactt ccaaataatt
     4861 tgacctgacc aggaccttca agacctgccg tccacatggt atttccaata tagatatttt
     4921 ccattggtac aaatttctca ataatatctt catgcccgat accatttaat agacataaga
     4981 cctctgtatc ttttttgatt aatgattgga tatcctgcag cattttttcc agctgcatcg
     5041 ctttggtaaa tagaataatc agatcaactt gatcttcttt ttctacttcg gattgaagaa
     5101 cgattggtag ttttgcttct acctctttac cattaaaatt tgcttgtaat ccatgttctt
     5161 tgatttgttg gatatgttct gcccagccat cgattaataa tacttcgttc ccactttggt
     5221 gaagcatcaa cccaaagcga cttcccattg cgccagcgcc tgcaattgct attttcattg
     5281 attcttcctc ctttttcttt ctattatact catttgagaa tcttttcaca acctcgtttt
     5341 gttattcttc tcttatttat catgtttata tccaccattc aagttatttt atcagcaaat
     5401 caataaaaac tggaatagaa tcccaatttc atcgtaagag aaactagact agcaataaga
     5461 aaatagaaac attcatagaa aaaatctcac tcctctgatt ataaaagagg gggcattaac
     5521 ctcgtgtata ctaaaacctt ccattcattc gatggatgat tttctttttg tgaaaaaata
     5581 ttcattgtag tacgatgttg cgcaacacag acggatggta ttgcttatta tttattaaaa
     5641 tcgaacaata cacttattta aaaacttctt tcccagattt tcttccattt ttacagttat
     5701 aactagtatt atcatgaaaa atagcctttt attgtctatg ggaataataa tttaaaattg
     5761 gtactttttt attaattagt tcatcgccca ataatctctc cagtttctta gcatctcctg
     5821 actcaaccgg aattatccaa gcccagtcta aataattttt taaaatctca tcatattttg
     5881 caaatatttc attgccatat tccgtcctta aaattatatt ttcagtgcca gacaagtgat
     5941 ttatattgag ttgataaaaa agatctccat ctaaaaaaat atcattagaa gaagatttcc
     6001 ccacagtaat gaccattcca tcagctctat gtgttacata caaataattg tatgacctca
     6061 tttcacctaa tgaatcaatt atttcttgcc ctttagctct aatatattga tctctattac
     6121 ctatctcact cttatttaac aaaagttttt tcatgtcaaa tttaatcggc tgttttattt
     6181 ttgtaaattc gcttctacta ctaatccaat ccgagattaa tttttcaaca tgtcccagag
     6241 taaactctga tttattcgct aaatactttt tttgttgtat taaagacatc tttccccgaa
     6301 gctcaaaatt gagcctacat cgattctcta tctcttcaaa aagcatctta tcttcatcat
     6361 tcttatattc atcattcata aaaccaatcc agctcctcga ttaagataat attttaatct
     6421 catacaaaaa ctagctctca tcaaaacaac cattgttttg atttctcatg aaatttcttt
     6481 atacttttct ccgattataa attcctaacc ccttgagaaa attacgcata tatttatctc
     6541 cgcaaggctt aaaatttcgt tggccttctt ttctcaaaaa tgccccgatt tcgccttttg
     6601 atggatagat ttctgcctca gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg
     6661 cgatttttaa tttttttagc aacacattgt tgatatattt gatttccttg cttttttgcg
     6721 gcgtgtctac tatcggctta tctttttgcg gaccacgcgc caagagaatc aggccattta
     6781 ggaatgcgtc aaaaacttgg ttgttgatgg ttttttcata cacattattt tctgcattat
     6841 ctttgttctt atcctgtact ttggtcagta ccgcctgagc ttcttcttta gtcagtggta
     6901 caccgcctag ctcaaaagct ttaatcaaat ccgtatcttt caaatcaatc gcataacgca
     6961 atcgcaataa cctatcattg ttattcattt gttcctccga agtatttagt ctttactttc
     7021 ttacgtactt tttgtcttgc taagattttt aggatcaatg ataaaaacc
//
LOCUS       DANKHK010000107         6967 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000107 DANKHK010000000
VERSION     DANKHK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            594..764
                     /locus_tag="PW109_002248"
     CDS             594..764
                     /locus_tag="PW109_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512041.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQEYAQDFIAYEKTISDKQFEIEKV
                     ALADWLLQPREF"
     gene            778..1395
                     /locus_tag="PW109_002249"
     CDS             778..1395
                     /locus_tag="PW109_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512042.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNAIQQKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKSKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRTRYNVTVDQRKNKGKRDS"
     gene            1395..3539
                     /locus_tag="PW109_002250"
     CDS             1395..3539
                     /locus_tag="PW109_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2512043.1"
                     /translation="MSTVILAEKPSQALAYAQAFNQSDKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGNYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIFQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGRITDV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITE
                     NEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QNKEEKEDVQTLPLVIIGEHAEVDVKSAEKETQPPKAFTEGTLLTAMKTAGKTIDDEE
                     AQKILKDTEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFITNIKKFIVHLLEAVPTDIEKLNFSDYQEQKEKE
                     AEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKNKEKKSYNAVVKIGEKGYIDFISFSK"
     gene            3642..4538
                     /locus_tag="PW109_002251"
     CDS             3642..4538
                     /locus_tag="PW109_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2512044.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILSNKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYKNVFFEMLERIIQLENEKE"
     gene            4636..4845
                     /locus_tag="PW109_002252"
     CDS             4636..4845
                     /locus_tag="PW109_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2512045.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            4862..5134
                     /locus_tag="PW109_002253"
     CDS             4862..5134
                     /locus_tag="PW109_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2512046.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            5136..5999
                     /locus_tag="PW109_002254"
     CDS             5136..5999
                     /locus_tag="PW109_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL2512047.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(6262..>6967)
                     /gene="erm(B)"
                     /locus_tag="PW109_002255"
     CDS             complement(6262..>6967)
                     /gene="erm(B)"
                     /locus_tag="PW109_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2512048.1"
                     /translation="LTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVT
                     SIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKV
                     VFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSV
                     LIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYE
                     QVLSIFNSYLLFNGRK"
BASE COUNT         2568 a         1076 c         1313 g         2010 t
ORIGIN      
        1 attttgtcat tggtatgggt ataaatcaat gcacttcgta taccttcgtt atatttgagt
       61 aaattttgta gttttttcta aattcttcgt ctaaactttc tgtaagaagt gggaatttag
      121 gtgcacggaa actacaccaa gttttttaat attccgtctg tttttagtta agaaacgcca
      181 atttactttc agtttctgtg cttcattttt gagccctttt aattgcagct ttttcatcat
      241 tcttacacga aaggcttgaa acggatgttc cagtgacctc gtttagagcg tacatttatt
      301 caagaaagtt taaataagat atcaaaatgg ccgaaaacag acacacaact cgatttattt
      361 tttagggtga aggagagagt agcacgaaaa tttttagtta gcttggactg aacgaagtga
      421 gggaaaggct actaaaacgt cgatgggcag tgggagcgaa gcacacactt accttttgaa
      481 cttttatttt atataggaca ttaaagtata cttatttgtc ctattgatta gatagcagta
      541 taatagcttt atagagtagg tcatttaagt tgagcataat aggaggatca agaatgaaaa
      601 aatttattta tcgagtttta gaaaatgacg aagtggtggc tatttttaat gagcaagaat
      661 atgcgcaaga ttttatcgct tacgaaaaga caatttctga taagcaattt gaaattgaaa
      721 aagtagcact cgcagattgg ttattgcaac cgagagaatt ttaggggttg gttgaaaatg
      781 gctaaaattg gttatgcacg tgtcagtagc aaagaacaga acttagatcg gcaattacaa
      841 gcgttacagg gcgtttctaa ggtcttttca gacaaattaa gcggtcaatc ggtcgaacgt
      901 ccacaattac aagctatgct taactatatt cgtgaagggg atattgttgt agttactgaa
      961 ttagatcgat taggacggaa taataaagaa ttaacagaat tgatgaatgc cattcaacaa
     1021 aaaggagcaa cgcttgaagt tttgaatttg ccctctatga atgggattga agatgaaaat
     1081 ttaaggcgtt tgattaataa tttagtgatt gaattatata agtatcaagc ggaatcagaa
     1141 cgcaaacgta ttaaagaacg acaagcccaa gggattgaaa tagctaagtc aaaaggaaaa
     1201 ttcaaaggtc gtcagcataa atttaaagaa aatgatccac gtttaaaaca tgcttttgat
     1261 ttatttttga acggttgttc cgataaagaa gttgaggaac aaactggaat taatcgcaga
     1321 acgtttagaa ggtatcgtac aagatacaac gtgacagtcg atcaaagaaa aaacaaggga
     1381 aagagggata gttaatgagt acagttattt tagctgaaaa accaagccag gcgttagcct
     1441 atgcccaggc atttaatcag agtgataaga aagacggata ttttgagatc aaagacccac
     1501 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac
     1561 caggtaatta tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg
     1621 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac
     1681 ttctcaaaaa ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata
     1741 tcgcctggtc gattatccat aaagcaaatg ccttttcaaa agataaaaca tttaaaagac
     1801 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaacctg
     1861 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1921 tcggcatgaa cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta
     1981 cattttcact aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag
     2041 ccatagagaa ttttaaaaaa gaaccttttt tcgaggtgga agctagtata aaagtaaacc
     2101 aagggtcgtt taagggcgtt ctaagcccca cacagcgttt taaaacccaa gaggagcttt
     2161 tagcttttgt ttcttctaaa caagctaaaa taggcaatca agagggcaga atcactgacg
     2221 tgcaaaccaa agagaaaaaa acgaatagtc ctagtctgtt ttctttaagt agtttgcaat
     2281 caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct atgcaagggc
     2341 tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt acagagaacg
     2401 aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga cttgatcttg
     2461 aaatggttca aacagagcct agaaagcgtt atgtggacgg tagtaaggta caggaacacc
     2521 acgccattat cccaacaaaa caagtaccta ccgaatctgc attagcgaaa atggacgatt
     2581 tacaacgaaa aatttatgct ttagtcgtta aaacgaccgt tgccatgttt ttacctgatt
     2641 acttgtatga agaaaccaag atacaaacca aagtagccga cttacttttt caatcaatag
     2701 gcaagacacc aaaacaagaa gggtggaaaa ttctttttaa acaacaaaat aaagaagaaa
     2761 aagaggacgt tcaaacgtta ccattggtta tcattggcga acatgccgaa gttgacgtta
     2821 agagtgccga gaaagaaaca caaccgccga aagcttttac agagggtaca cttttaaccg
     2881 ctatgaaaac cgctggtaaa accattgatg atgaagaagc tcaaaagatt ttaaaagata
     2941 cggagggtat cggaacagaa gcgacaagag caagcattat tgaagccttg aaacaaaaag
     3001 aatatatcca agtcattaaa aataaacttg ttgtgactga aaaaggaaaa ttattgtgcc
     3061 aggcagttga aagtcagcac ctcttaacga gtgctgaaat gacggctaaa tgggaaacgt
     3121 atttaaaaaa aatcggtaaa agagaaggca atcaagagaa ctttattacg aatatcaaaa
     3181 aattcattgt tcatttactg gaagctgtac ctaccgatat agaaaaacta aatttttctg
     3241 attaccagga acagaaagaa aaagaagcag aaaaaagtat tgtaggcaaa tgtcctaagt
     3301 gcggcaataa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
     3361 agttcacttt agctgaacat tttagaaaga aaaaactcac taaaacaaat gtaaaagaat
     3421 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa taaagagaaa aagtcctaca
     3481 atgccgttgt aaaaatcggg gaaaagggat atattgattt tatttcattc tcaaaataag
     3541 cataaaagcc ctttaaatag ggcttttata tattaatcac aaatcactta tcacaaatca
     3601 cttatcacaa atcacaaatc acttgtgatt tgtgattttt aatgatacaa tattactata
     3661 caaaaaaaga atggggcgta gttatggaga aggaagaact caaaatactt gaagaattaa
     3721 gacgtatttt aagcaataaa aatgaagcaa ttgttatttt gaataattac tttaaaggtg
     3781 gtgttggaaa gtcaaaatta tcaactatgt ttgcttactt gacagacaaa ttgaatttaa
     3841 aagttttaat gatcgataag gacttacaag caacattgac aaaagactta gcaaaaactt
     3901 ttaaggtaga attgccacgt gttaattttt atgaaggctt gaaaaatgga aacttggctt
     3961 cttctattgt tcatttgact gataatttag acttgatccc tggcacgttt gatttgatgt
     4021 tactgccaaa attaactcgt tcatggacgt ttgaaaatga aagtagattg cttgctactc
     4081 ttttagcacc tttaaaaagt gactatgatc tcattattat tgatactgta ccaacgccaa
     4141 gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct ttacaagcag
     4201 aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat ttacaagaac
     4261 aatttaaccc tggactagat atgatcggtt ttgttcctta tttagttgat acggacagcg
     4321 caacgataaa atcaaacctg gaagaactgt ataagcaaca taaagaagat aacttggttt
     4381 tccaaaatat tatcaagcga agtaataaag taagtacctg gtctaaaaac ggcattacag
     4441 agcataaagg ctatgacaaa aaagttttat ccatgtataa gaacgtattt tttgaaatgc
     4501 ttgagcgaat tattcaatta gaaaacgaaa aagaatagaa tcacaaatca caagtgatta
     4561 atcacaaatc acttgtgatt tgtgattgtt gatgataaaa taagagtaag aagaaataga
     4621 aagaagtgag tgattatggg aaatttaggc gcacaaaaag caaaacgaaa tgatacacca
     4681 atcagtgcaa aaaaagatat aatgggagat aagacggttc gtgttcgtgc tgacttgcac
     4741 catatcataa aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggatcaa
     4801 gccttagaag aatatatacg gaaatattta cctgacaaac tttaaaagga gtgaaaatga
     4861 aatggcagtt acgtatgaaa aaacatttga aatagagatc attaacgaat tatcggcaag
     4921 cgtttataat cgagtattaa actatgtttt gaaccatgaa ttaaataaaa atgactctca
     4981 attattggaa gtcaatttat taaaccaatt aaagcttgca aaacgtgtaa atctttttga
     5041 ttattcttta gaagaattac aagccgttca tgagtattgg cggtcaatga atcgttactc
     5101 aaaacaagtt ttgaataaag agaaagtggc ttaatatggc aaatatagtc aattttactg
     5161 acaaacaatt tgagaatcgc ttaaatgata atttagaaga attgattcaa ggaaaaaaag
     5221 cggttgaatc gccaaccgct tttttacttg gtgggcaacc agggtcaggg aaaaccagtt
     5281 tgcgatcagc aatttttgaa gaaacacaag ggaatgttat tgtcattgat aatgatacct
     5341 ttaaacaaca gcaccctaat tttgatgaac tagtgaaact ttatgaaaaa gacgtagtaa
     5401 aacacgttac cccttattct aatcgcatga cagaagcgat cataagccgt ttgagcgatc
     5461 aagggtataa tttggtgatc gaaggtacag gacgaacaac agacgttcct attcaaaccg
     5521 caacaatgct tcaagccaaa ggttatgaaa caaaaatgta tgtcatggca gtacctaaaa
     5581 tcaactcata tttaggaaca attgaacgat atgaaaccat gtatgcagat gatccaatga
     5641 cagccagggc aacaccaaaa caagcgcatg atattgttgt caaaaactta ccgaccaatt
     5701 tagaaaccct tcataaaacg ggcttattta gcgatataag gctatataac agagaaggag
     5761 taaaactcta ttcaagtttg gaaacacctt ctattagtcc aaaagaaacc ttagaaaaag
     5821 aattgaatcg taaagtatca gggaaagaaa ttcaaccgac tttagaacga atagagcaaa
     5881 aaatggttct aaataaacac caagagacac ctgaatttaa agcaattcaa caaaaattgg
     5941 aaagcttgca gccacctaca ccaccaatac ccaaaacacc taaacttcca ggtatttaaa
     6001 ccttaaaaaa ggaaaagagt agttaccaaa aaacggtaac tactcttttt ttataaaaac
     6061 atttcctcat gttggggtcc cgagcgccta cgaggaattt gtatcgataa gaaatagatt
     6121 taaaaatttc gctgtttttt tctaatttca ttttgaggtg aattaattgg tagttattag
     6181 aggtgcccta tacctaataa tttatctaca ttccctttag taacgtgtaa ctttccaaat
     6241 ttacaaaagc gactcataga attatttcct cccgttaaat aatagataac tattaaaaat
     6301 agacaatact tgctcataag taacggtact taaattgttt actttggcgt gtttcattgc
     6361 ttgatgaaac tgatttttag taaacagttg acgatattct cgattgaccc attttgaaac
     6421 aaagtacgta tatagcttcc aatatttatc tggaacatct gtggtatggc gggtaagttt
     6481 tattaagaca ctgtttactt ttggtttagg atgaaagcat tccgctggca gcttaagcaa
     6541 ttgctgaatc gagacttgag tgtgcaagag caaccctagt gttcggtgaa tatccaaggt
     6601 acgcttgtag aatccttctt caacaatcag atagatgtca gacgcatggc tttcaaaaac
     6661 cactttttta ataatttgtg tgcttaaatg gtaaggaata ttcccaacaa ttttatacct
     6721 ctgtttgtta gggaattgaa actgtagaat atcttggtga attaaagtga cacgagtatt
     6781 cagttttaat ttttctgacg ataagttgaa tagatgactg tctaattcaa tagacgttac
     6841 ctgtttactt attttagcca gtttcgtcgt taaatgccct ttacctgttc caatttcgta
     6901 aacggtatcg gtttctttta aattcaattg ttttattatt tggttgagta ctttttcact
     6961 cgttaaa
//
LOCUS       DANKHK010000108         6750 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000108 DANKHK010000000
VERSION     DANKHK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            71..2110
                     /locus_tag="PW109_002256"
     CDS             71..2110
                     /locus_tag="PW109_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2512049.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSDDVSQVDLKTSMIYSDNIYMAQETLKMGEKKLRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVY
                     PKLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2219..3370)
                     /locus_tag="PW109_002257"
     CDS             complement(2219..3370)
                     /locus_tag="PW109_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2512050.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(3524..4054)
                     /locus_tag="PW109_002258"
     CDS             complement(3524..4054)
                     /locus_tag="PW109_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2512051.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4153..4503)
                     /locus_tag="PW109_002259"
     CDS             complement(4153..4503)
                     /locus_tag="PW109_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2512052.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(4660..6189)
                     /gene="ahpF"
                     /locus_tag="PW109_002260"
     CDS             complement(4660..6189)
                     /gene="ahpF"
                     /locus_tag="PW109_002260"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2512053.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6199..>6750)
                     /gene="ahpC"
                     /locus_tag="PW109_002261"
     CDS             complement(6199..>6750)
                     /gene="ahpC"
                     /locus_tag="PW109_002261"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2512054.1"
                     /translation="GKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCP
                     TELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISR
                     IFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGE
                     VCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2319 a         1323 c         1208 g         1900 t
ORIGIN      
        1 tttattatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
       61 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      121 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      181 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      241 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
      301 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
      361 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
      421 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
      481 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
      541 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
      601 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
      661 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
      721 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
      781 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
      841 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
      901 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
      961 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1021 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1081 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1141 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1201 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1261 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     1321 ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg acaacggcac
     1381 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     1441 gggatcttat caagtaacgc gtgtcagtga tgatgtatca caagtagact taaaaacttc
     1501 tatgatctat tccgataata tatatatggc acaagaaacg ttgaaaatgg gtgagaaaaa
     1561 attacgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     1621 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     1681 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     1741 ttttgccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     1801 taagaagaat gtcatcggcg aaacagcatt acaaacgatc gtgccagatc tgagagaagt
     1861 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     1921 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggaaag agaacagttt
     1981 cttgtttgct ttcaatcctg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2041 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2101 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2161 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2221 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     2281 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     2341 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     2401 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     2461 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     2521 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     2581 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     2641 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     2701 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     2761 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     2821 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     2881 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     2941 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3001 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3061 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3121 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3181 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3241 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     3301 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     3361 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     3421 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     3481 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     3541 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     3601 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     3661 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     3721 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     3781 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     3841 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     3901 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     3961 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4021 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4081 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4141 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4201 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4261 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     4321 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     4381 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     4441 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     4501 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     4561 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     4621 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     4681 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     4741 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     4801 ggtcactatt tcatttcttg gattcatttc gatcgttcta tctagccaat ccgtattagg
     4861 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     4921 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     4981 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5041 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5101 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5161 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5221 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     5281 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     5341 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     5401 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     5461 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     5521 agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag gttctttttc
     5581 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     5641 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     5701 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     5761 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     5821 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     5881 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     5941 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6001 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6061 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6121 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6181 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6241 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     6301 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     6361 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     6421 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     6481 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     6541 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     6601 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     6661 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     6721 atgtgcatta aattcttcga tttttttccc
//
LOCUS       DANKHK010000109         6429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000109 DANKHK010000000
VERSION     DANKHK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            327..893
                     /gene="amaP"
                     /locus_tag="PW109_002262"
     CDS             327..893
                     /gene="amaP"
                     /locus_tag="PW109_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2512055.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTRVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            906..1094
                     /locus_tag="PW109_002263"
     CDS             906..1094
                     /locus_tag="PW109_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2512056.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQTGIVDQFFKS"
     gene            1107..1664
                     /locus_tag="PW109_002264"
     CDS             1107..1664
                     /locus_tag="PW109_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2512057.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1696..1938
                     /locus_tag="PW109_002265"
     CDS             1696..1938
                     /locus_tag="PW109_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2512058.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2279..3493
                     /locus_tag="PW109_002266"
     CDS             2279..3493
                     /locus_tag="PW109_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2512059.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3524..4951
                     /locus_tag="PW109_002267"
     CDS             3524..4951
                     /locus_tag="PW109_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2512060.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTTLSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMVANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4974..5900
                     /gene="glsA"
                     /locus_tag="PW109_002268"
     CDS             4974..5900
                     /gene="glsA"
                     /locus_tag="PW109_002268"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2512061.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WGGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6108..>6429
                     /locus_tag="PW109_002269"
     CDS             6108..>6429
                     /locus_tag="PW109_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512062.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFDGIF
                     TSTEL"
BASE COUNT         2052 a         1083 c         1344 g         1950 t
ORIGIN      
        1 taaagtaact aaagttattt attgtatttt ttaattctcc gaaaaaattt aataaataaa
       61 tagaatcttg taataataac tgagcataat ctttgcaatt tttaatcttg aataatatcc
      121 tttaagtata gccagcaatc aaaagtaaat agagaataag taagcaattg ttatttcagt
      181 atcagactgg caataaaacg ccgttatgat ccagcaatgt ttggtagcgc tttatttact
      241 ggtgctatgc taactgttcg ctatctatac tgtgagtgtc ggtagagtta tttacagggt
      301 aaaaataagt ttataggagg tggaaaatga ataaggggtt aaaaataatc ggtgttatca
      361 tgagtttact gctactttca gtatttctag cgattgcgtt gatcaatgcg ccgtatatta
      421 tgcctgaaca attggaacga tttcgcttct ttactttaac gaattactac atgcaacaat
      481 atattttctg ggcagctgta gtatttgccg ttcttgctat tatcatcttg ctagtcgttc
      541 ttttctatcc aaagtcaaga ggaactttcg ttatgaaacg ggaagatggc aaactaacaa
      601 tcgacaaaaa agcgatcgaa gggttggtac gttcccattt acacgaagaa gagttcattc
      661 attcacctaa agtacggata cgttcaacga aaaatcgaat ccatatccat gtcaacggag
      721 acttgaaacg aacttcttca ttagtaggga aaacaggtgt attgatgcaa gacatcgaag
      781 aagaagtgac tagagtactt ggaacaaaag aaacggtcaa agtggcagtc acatacagcg
      841 gttatgaaga acaagaagat acaagagact acaagcactc tcgagtggaa taggagggcc
      901 aaataatgaa agaactattt tcagcataca agctccctat tatctttggg gggcttggac
      961 tactgctagc tgttttatgg ttaacgattg gatttttcaa gacattgttg atcataatca
     1021 tgacagcaat tggcgcagga atcggttttt atttgaaaca gacaggaata gttgatcaat
     1081 tttttaaatc ataggaggaa acaaagatgg aaaacaaaaa catgaataca acaaatggat
     1141 cagttttggc aaatgaaaaa ggagaaaacc gcatgatgaa tggtgaattg acttatgaag
     1201 acaaagtcat ccaaaaaatc atcggttatg cattgaaaga tgtagatggt ctattgacat
     1261 tagatggcgg atttttctca aatatggctg aaaaattagt caacacagat aacgtaacag
     1321 caggtatcaa tacagaagta ggtaagaaac aagtagctgt ggatctagat gtgatcgtag
     1381 aatacggtaa agacatcgaa gatatttaca accaaatcaa agaattaatc agtactgaag
     1441 taaacaatat gacacattta gatgttatcg aagtgaatgt gaatgttgca gatatcaaaa
     1501 cacaagaaga ataccaaaaa gatcaagaaa cagtacaaga caaagtaaca gaagcagcaa
     1561 aatcaacagg acaatttgct tcacgccaaa cagataaagc aaaatctgct gtaggcaaag
     1621 gtgcacaaaa agtcaaagaa aataacgaac cacgtgtaca ataagaagta agaagtcagt
     1681 taggaggaat ttaaaatggg atttatttgg gcattaatcg tcggcggtat cattggagca
     1741 attgcaggag caatcacgaa taaaggatca tcaatgggaa tcatcgcaaa catcattgct
     1801 ggtttagtag gttcagcaat tggacaagct ttactaggga cttggggacc aacaattgca
     1861 ggaatggcta tcattccatc attgatcggt gcaatcattt tagtattagt cgtttcattc
     1921 tttgcacgta aattataagc aaattgaaga aaaacttatg ttgaaatgaa gaacaaattt
     1981 gagaaccgta gcgaaactcg ctgcggttct tttttatata ttttatatga agtattcttt
     2041 attccttatg aaaaaatatc tttaaaaata aaaaaagacg aaaataaacg gttttctata
     2101 aaaagaagac cgattatttt ttgtctttat ttgtgtgaaa aacgaaaaag agcacaacac
     2161 atcaagcttt tattaattgt ctgtctttta taaatgcaag tattaattca aaaaaacaga
     2221 agaaaaaaac aactacacct tcttttatac tcgtaatcgt agaaaggagg cgaaaaaaat
     2281 gaatacatta cctatcaata caggagatac agcattcatg attttatgta cagcaatggt
     2341 ttgcctgatg actcctggtc tagcattttt ttacggcgga ttagcacgga agaataatat
     2401 actaacgatc atgtctcaat cttttctctc cgttgggata acaacgatta tgtgggtatt
     2461 tggtggattc ggattggcct ttggacggga tatcggcggt gttattggaa acccggcaga
     2521 tttctttttg atgagacatg ttacaagctt ccccaacatg tttcatggtg caacgattcc
     2581 atttttaatg ttctttcttt accaactgat gtttatcgtg attaccgttc ctttgatgac
     2641 aggagctttt gcaggaagat tgaatttaaa aggctatatt atacttgtta ttttttggaa
     2701 tctgctgatt tatttccctg tttgccactg gatatggggc ggcggatttt tggatcagat
     2761 gggatttaga gatttcgctg gtggagcagt catccacacg acagctggat tcggctcttt
     2821 agcatgtatc ctcacacttg gtcaaagaaa gattccttta ggagaaagaa gtcagcatag
     2881 cagtcttatg gcagctgcta tcggtacagg actgctttgg tttggctggt tcggttttaa
     2941 ttccggtggt gcacttcgag cagatgaaca agcagtcaat gcatttggtt caacattcat
     3001 cgctcttgca tttgcgatga ttacttggct gatcattgca aaagtaaaag gaaatggctt
     3061 tgattttgtc gacgtactaa caggatccgt tgctggattg gcagccatta cgccttgtgc
     3121 cggttatgta gaagcaaaaa gtgccatgct gattgggatc attgcgggta tcgtctgtca
     3181 tgctgctgtt gattttcgta aaaagaaaca atgggatgat gcacttgatg tgtggggtgt
     3241 tcatggcatg ggcggattca caggtacgat tctgataggg atctttgcat ctaacagcca
     3301 tctactaaca aatccaagca gctggtactt cttagggatt caagtgattg gtgtcatcat
     3361 tactgccgtg tatgcctatg tgctgacaac agtgatttta aaatcggcga tgcattttac
     3421 aacgatcact actacaaaag aagaacaaga agaaggattg gactttttct tctttggcaa
     3481 caaactcaat tgaaacaaat tcaaatagaa aagaggaaga ttcatgcaac aaagcaaaac
     3541 gaaaattcct gcaaaaaaat taacgttctt tggctttttg gctatgacca tctcaatggt
     3601 cgtttcatta tatgaatatc ccacattcgc gacctcaggc ttcagcttag tatttttctt
     3661 gttattaggc ggtttgctat ggtttatccc tgtggcattg tgcgcagcag agatggcaac
     3721 ggtgaaagga tgggaaaaag gcggtgtcta tacttgggtc agtcgcacat tgggaaaacg
     3781 attcggtttt gccgcaatct ttttccaatg gtttgagata acagttggct acttgaccat
     3841 gctgtatttc ttaactggtg cattatccta tgcaacgggt attggtgcta tacaaaacaa
     3901 taaatatctg aaattggcaa tcctattgat tattttctgg gctattttac tctcacagtt
     3961 aaaaggaacc aagtacactt cattgattgc tcggatcggt tttgtagctg gtatcttgtt
     4021 gccagcatta gtcctgtttg gcttagggat ccattatgtc gcttcaggcg ctccactaca
     4081 aacgacactg tctatgaaaa cattgattcc tgatttttcg aaattgccta cgctggttgt
     4141 ttttgtttcc tttattttag catatatggg cgtagaaaca tctgccagtc atgcaaatga
     4201 aatggaaaac cctaaaaagg attatccatt agcaatgttt gtattggtta ttctggcaat
     4261 cattttagat acttttggcg gattgaccgt cgctactaca gtacctcaac atggtttgtc
     4321 tcttaataca ggggtgattc aatcgttaga attcctatta agacacattg atccaaatct
     4381 tgcatggatt gcgaaaattc ttgcgattct tgtttgttta ggtgtcatcg gagaaattgc
     4441 ttcttgggta acctctcctt caaaagcact tcacgtagca gcagaagaag gattacttcc
     4501 tgaatatttt gcaaaagaaa acacacacgg tgttcctgct catttgatgg tcgccaatgg
     4561 gatcgttgca acaatctggg cagccgtctt tacattaagc ggcgggggga ataatctttc
     4621 atttcttgca gcgatgtcat tgactgttgt aatctacctg atgttgtact ttatgttttt
     4681 cgcgggctat ttgaagctgg tttttaaaga tgacagtctt gaaagagcct atcaggttcc
     4741 aggcggaaaa gttgtaaaag taatcgttgc ttgtttaggt cttttgactt cactttttgc
     4801 tttttgtatc tcatttgttc cacctgctag tatcgcagct ggacagcatg ggatttatga
     4861 aacgattctg attgtttctt atcttatcgt cttagttatt ccatttacga tctacgcatg
     4921 ccgtcataaa ttccagcatg cagaaaaata aaaaatgaaa gctggttatt caaatggaag
     4981 taaaagaatt agaacaattg attggaaaaa atttccacta ttatcaaaca ggacaagtgg
     5041 cggattacat tccagcatta gctgaagtgg atcctaaaca attaggtatg gcgatttatg
     5101 atttggatac aaaacaactg attgagggag gagatgccga tgtgcgcttt gccatcgaaa
     5161 gcatgtcaaa agttcccgta ttgttattag caatcaaaga caacggaata gaaaaagtct
     5221 ttcaaacaat caacacggaa ccaaccggtt ttgccttcaa ttctatcatg aatatggaaa
     5281 tcaatcatcg caaacatcca atgaatcctt ttgttaatgc aggagcgatt gcaacaacct
     5341 cgttgattgc cggtaaagat gcagatgaaa aatttgagcg aatcttgtca tttatgaaag
     5401 agatctgtga tgatcctaat atcacattga atgaagaaat ctatcattct gaatcaagaa
     5461 caggtgatat caatcgctct cttgcttact atatgaaagg taatcaaatg atcgaaggag
     5521 acgtaccgga gatactcgat gcttatttca gacaatgctc cctcaatgtc accgctacag
     5581 gcatagcaaa attagctgct gtgctggcaa ataaaggaat tgctccttgg ggtggcaaac
     5641 aaatcattac tgaagaaagt gctacaatcg tcaaatcaat tatgacaact gctggtttgt
     5701 atgatgaatc aggagagttt tccgtccatg ttggtgtacc tgcaaaaagc ggtgtaggcg
     5761 gcgggttgat ggcagctgtg ccaaatcgtt acggcatagg tgtcttcagc ccggcactag
     5821 atccatttgg taacagcgca gctggtatcc aattgttgaa agatgtagtc aaagaactag
     5881 acgctgacat ctttgaataa ccattcttga aagaggctgg ggtaaaagtc caaacggctc
     5941 aaagttacct acaatgaggc tgaaattgtg agcttgtttg acttttgtcc cagtttcttt
     6001 ttttattggt ttttgaaagg gcttttctaa ataaaaagga aacaaaaata tgatttacag
     6061 atatgattaa ataaactcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt
     6121 acatcgagcg aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag
     6181 aaatagatat cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg
     6241 atctagaaag tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc
     6301 ataagtataa actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt
     6361 acggtcaatc ttatttttta aagtttttaa gttattattt tgatggtata ttcacgagta
     6421 cagaactgg
//
LOCUS       DANKHK010000110         6333 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000110 DANKHK010000000
VERSION     DANKHK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(99..1229)
                     /locus_tag="PW109_002270"
     CDS             complement(99..1229)
                     /locus_tag="PW109_002270"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2512063.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PW109_002271"
     CDS             complement(1878..3080)
                     /locus_tag="PW109_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2512064.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PW109_002272"
     CDS             complement(3140..3343)
                     /locus_tag="PW109_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2512065.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PW109_002273"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PW109_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PW109_002274"
     CDS             4880..5692
                     /locus_tag="PW109_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512066.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1888 a         1300 c          998 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaa
//
LOCUS       DANKHK010000111         6183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000111 DANKHK010000000
VERSION     DANKHK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            58..1566
                     /locus_tag="PW109_002275"
     CDS             58..1566
                     /locus_tag="PW109_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL2512067.1"
                     /translation="MKNNKYIWLAATISMLTLGGVFILIQQQNKEEHAEVVFQEEQRQ
                     ISSRMSNMMGDNQEADLKVVHQTERQLLIPPVLEPSSKSKENVIYDIVAQNGEVQIMD
                     GEKTETLGYNGDFLGPVIRLKKGQKVTINTNNNLDASTSFHWHGLKVASDADGGPHQI
                     IEAGQKKSVTFEVDQEASTLWFHPHPEGETASQVYKGLAGLMYIDDDNSKSLDLPSKY
                     GVDDIPLIVQDKSFSSTNQINYENDFNSDGTKGETLLTNGTINPYIEIKNRWMRYRIV
                     NGSNSRNFTFTLDKDESFYQIATDGGFLNTSVKLSKLLLAPGERAEILVDTQNYKKGE
                     VIHLLANNLVALTMRIENTIENKEFTPSDSLNTISTLDEKKLEDLARQSINLRGMSHM
                     VNINNKQFDMERIDLYKKLGTQEIWEVNNISSMMGGMIHPFHIHGVQFQILSRDGNQP
                     ALNEQGWKDTVLVNPDETVELLVKFDREGIFMYHCHILEHEEYGMMGQMEIK"
     gene            1594..2274
                     /locus_tag="PW109_002276"
     CDS             1594..2274
                     /locus_tag="PW109_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2512068.1"
                     /translation="MKILIVDDEEKILEIIDAYLVANHYSVYKAISGAMALEKFEKNN
                     PDLVVLDLMLPDTDGLTVCRKIREVSNVPIIMLTAKSDEDDILTGLKLGADDYMIKPF
                     SPKELVARIQTVLRRTETLSNPNKLSSSNGELIVYPESRQVYLHQVELNLTTTEFDIL
                     HALISTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHIKNLRHKIENDAKSPMYILTVHG
                     TGYRFGGH"
     gene            2274..3668
                     /locus_tag="PW109_002277"
     CDS             2274..3668
                     /locus_tag="PW109_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2512069.1"
                     /translation="MRKRTIKTQLVLSFLAIATLIIGSISLVALSLTNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKALENNIYFSIEDEQGNMVWELKGK
                     NLKSAQDKLKKNALKVSEKKSVKLDETIEAKKKLINDGNEFGKVTFYYFGPFAYTEHD
                     ALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTRKLTRGEYADKIPQ
                     ETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGVW
                     KITPERLQSCYDEIDRLTRLIGNIEVINKIEAKYDHLNKTEFNIYKLIESVIENFASK
                     IESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKNHVLL
                     IVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKSK
                     LGLGTKFVIKLPFQ"
     gene            3683..3889
                     /locus_tag="PW109_002278"
     CDS             3683..3889
                     /locus_tag="PW109_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512070.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            3910..4506
                     /locus_tag="PW109_002279"
     CDS             3910..4506
                     /locus_tag="PW109_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL2512071.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSKSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            complement(4704..6005)
                     /locus_tag="PW109_002280"
                     /pseudo
     CDS             complement(4704..6005)
                     /locus_tag="PW109_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         2147 a          928 c         1228 g         1880 t
ORIGIN      
        1 aaaatctcca catatttttt ttatggtaac tacataatag atatgggaga tgatgggttg
       61 aagaataata aatatatttg gttagcagca acaatatcaa tgttgaccct aggcggtgtt
      121 ttcatattga ttcaacagca gaacaaggag gagcatgctg aagttgtctt tcaagaagaa
      181 caacgacaaa tctcaagtag aatgtccaat atgatggggg ataatcaaga agctgatttg
      241 aaggtagtac accaaacgga gagacaacta ttgattcccc ctgttcttga accatcttca
      301 aaaagcaaag aaaatgtgat atatgatata gttgctcaaa acggagaagt tcaaataatg
      361 gatggagaaa aaactgaaac attaggctat aacggggatt ttttaggacc agttattaga
      421 ctgaaaaaag gtcaaaaagt tactatcaat acaaacaata atcttgatgc tagcacatct
      481 tttcattggc acggtttgaa agtagcttct gatgcagatg gaggtccaca tcagattata
      541 gaagcaggac agaaaaaaag tgtaacattc gaggttgatc aagaagcttc tacactctgg
      601 tttcatcccc atccggaagg agaaacagca tctcaagttt ataagggttt agcaggtttg
      661 atgtatattg atgatgacaa ctcaaaatca ttagatttac cttccaaata tggtgtcgac
      721 gacattccct tgattgtgca agataaatct ttcagctcca ctaatcaaat taactatgaa
      781 aatgatttta actctgatgg aacaaaaggg gagacgttgc ttactaatgg cactatcaat
      841 ccatatattg agataaaaaa ccgatggatg aggtatagaa ttgttaatgg atctaattca
      901 agaaacttca cttttacttt ggataaagat gaatcattct accaaattgc tacagatggt
      961 ggttttctga atacttctgt taaacttagt aaactacttt tagcccccgg ggaaagggca
     1021 gaaatattag tggatactca gaattataaa aaaggtgagg taattcattt actagcaaat
     1081 aatttggttg ccttgacaat gagaattgag aatacgatag aaaacaaaga gtttactccc
     1141 tctgatagtc taaatactat tagtacttta gatgaaaaga aattggaaga tttagcaaga
     1201 caaagtatta atttaagagg aatgtctcac atggtaaaca tcaacaataa gcaatttgat
     1261 atggaaagaa tagatttata taaaaaatta ggtactcaag agatttggga agttaacaat
     1321 attagtagta tgatgggagg catgattcat ccgttccata tccatggtgt tcagtttcaa
     1381 attttatcta gagatggaaa tcagcccgca ctaaacgagc aaggatggaa agatactgtg
     1441 ctggtaaatc cggatgaaac agtagaactt cttgtgaaat tcgaccgtga aggtattttc
     1501 atgtatcatt gtcatatatt agaacatgaa gagtatggaa tgatgggaca aatggaaatt
     1561 aaatagaaaa taatttaaga aggatgattg tctatgaaaa ttcttattgt ggacgatgaa
     1621 gaaaaaatat tagaaattat tgatgcctat ctagttgcta atcattattc agtttataaa
     1681 gcaataagtg gcgcaatggc attggaaaag ttcgagaaaa ataatcctga tttagttgtg
     1741 ttagatttaa tgcttccgga tactgatgga ttgactgtgt gtagaaaaat cagagaagta
     1801 tcaaatgtac caattataat gttaacagct aagtctgatg aggatgatat tcttactggt
     1861 ctcaagttag gtgccgatga ctatatgatt aaaccattta gtcccaaaga attagttgcg
     1921 agaatacaaa ctgtattgcg cagaactgag accttatcaa atcctaataa gctttcttca
     1981 agtaatgggg aattgatagt atatcctgaa agtcgccaag tttatctaca tcaagtggaa
     2041 ttaaacctca caacaactga atttgatatt ctacacgccc taatatccac acctaataaa
     2101 gtcttttctc gtagtgattt gatagagaaa gttaagggaa tagaatttga tggactagat
     2161 aggagcatcg attctcatat aaaaaactta agacataaaa ttgagaatga tgctaaatca
     2221 cctatgtata ttttgacagt ccatggtaca ggatatagat ttggtggcca ctaatgagaa
     2281 aaagaacgat aaaaactcaa ttagttcttt cctttttagc aatcgcaaca ttaataatag
     2341 gatcgattag tttagtcgca ttaagtttga cgaacaatca ttttagtaaa tacgtagaag
     2401 agcgacaaga agatttgttg aatcagtatg tatatacaat tgatttattg tggttaaata
     2461 gtggagaaac atggaacagc gaggaacttg cagcactttc tgagaaagca ttagaaaata
     2521 atatttattt ttctatagaa gacgaacaag gcaacatggt ttgggaactt aaaggaaaaa
     2581 atttgaagtc agcacaggac aaactgaaaa aaaatgcctt gaaagtttct gagaaaaaaa
     2641 gtgtgaaact agacgaaaca atagaagcga aaaagaagtt aattaatgat ggaaacgagt
     2701 ttggaaaagt aacgttttat tatttcggac cttttgctta tactgagcac gatgccttgt
     2761 ttatctccag tatgaaacaa agtttaatgt atgttgctat tgcagctctt cttgtatcat
     2821 ttatacttgc ttcgtggatc tctgctagat taggattacc actaaagcat gtaagcgact
     2881 ttacacgtaa attaacccgt ggggaatatg ctgataaaat cccacaagaa acttcaatta
     2941 ttgaaattaa ttccttgatt gattccctaa atgatttaag taatcagttg gagaaacagc
     3001 atggacttcg aaaaagatta actactgata tttcacatga attaagaact ccattagcta
     3061 cccttaaagg caatgtggaa ggaatgattg acggagtctg gaaaataact cccgagcgcc
     3121 tacaatcttg ttatgatgaa atagatcgtc taactcgttt aattggaaat atagaagtaa
     3181 tcaacaaaat tgaagcaaaa tatgatcatt taaataaaac tgagtttaac atatataagt
     3241 tgattgaatc cgttattgaa aatttcgcca gcaaaattga gtcaaaaaac cttcatgttg
     3301 aaatccaagg ggataacatc aatatatctg cagataaaga taaaatgaac caagttgtta
     3361 tcaatctttt gaataatgct attaaattta cgcaaaagga gggaaccatt aagttttcta
     3421 taagtaaaaa taaaaaccat gttcttttaa ttgttgaaga caacggcatc ggtatagaaa
     3481 aagatcagca acttcatatt tttgatagat tttatatggc tgacccctcc agaagtagag
     3541 ctttaggggg gcaaggtatc ggattagcta ttgtaaagag cgttattgaa gcgcataaag
     3601 gtagtataac tgtaaaaagt aaattaggtc taggaactaa atttgttata aagttaccat
     3661 ttcaataaag gaggagagtt atttgtttca aaaacagata aaaattgcta aactagtatg
     3721 tgaatgttgg gcaaaaaaaa tattttttca actgatttcg atagatagtg tcgaaaaagt
     3781 tcatttagat atcttatcaa attctgtagt aatcttttct aagaaggact tatcaatagc
     3841 tacaattaaa aaagaagttt ttggattaca aatttatgag atgacctaaa tgtgattaat
     3901 taaaggagga tgaagacagt gaaaaagatg acaaaattag tatttgcaag tcttggaatt
     3961 attgtatttt taggagcgtg tagcaatcaa agtgaatcga ataattctaa aagcacaaac
     4021 gaggaatcga cttctacagc gtctagtgaa atgaatagta tggagggaat gaatcacgaa
     4081 ggtatggttc cttcttcaat gaaagatgct gcgaacccta aattcccggt gggaagtaat
     4141 gttatactgc taggcgatca tatggagggg atgaaaggtg caaatgctca agttgtaggg
     4201 gcctttgata ctacaatgta tgaagtaagc tatgagccaa aaactggggg accaatggtt
     4261 aacaatcatc gatgggtagt acaagaagag ttaaaagata ctgagactgt agcaaatgag
     4321 ggcgatactg ttatcttgaa tgccgatcac atggatggga tgatgggagc tgaagcaaaa
     4381 gtagataaat ctattacagg aactgtttac gttgttaatt acactccaac cgatggtcaa
     4441 gaagaagtta agaatcatat gtgggtaact gaagatgaga tggaatatga cgaaaataat
     4501 gagtaatttt atatactaaa ccaataagct ggtacatggc cttcgtattc tcaacatgag
     4561 gaatatgagc tctttgttaa taaggactga tgagttgtgc aaaatcaaat ctgggtcaga
     4621 acgaacctca ttctggctca gatttttggc tcttcgacat ttagtgctgg tagagagaca
     4681 actgaaaatt tagtcataac cagttaggca gagtgcttaa ctggtttttg ttttacttgg
     4741 aagatcaagc cttgaatgag ataaattctt gctcgaaagt tgtggaaatt tcgatagccg
     4801 taggctactc gttttatcac ttttatctta ttatttgttc tttccaatgc accattacta
     4861 tacctgggtt ttaaagcata ttgaatccct aatttgtatt tatttaaaaa cgtcagcttc
     4921 ttacaaaacc attgtggaag gcattgatct aagctgttga tcgtatcgaa aaaactttgg
     4981 ttgtccttgt gtttaaagtg atagaggagg agctggcaag tctcgtaagc cctggttaat
     5041 tgctcgtcgt aactcaaaag ctcatcgacc atcgcttttt gcgtcattgg tctgcgaaag
     5101 gatcgatcat attgataatc ggaactatcc agatcatagg catgcgtaag gagtaatttc
     5161 cagaatcgct tcaatcgtca atactttgtc ggttccgttt tttgaaacct gttcattgtt
     5221 ttgattcgca actgatttaa cgtacgagtg atttgttgga caatgtgaaa gcgatcggtc
     5281 acgattttgg cgtttgggaa gaccgctttt acaagttcaa aataaggggc attcatatcc
     5341 atcacgacgt atttcacgtt cgctcgtgct tttctagtga atttaaggaa atatggtttt
     5401 aagaaagcaa ggcgacgatt ctccaataca ccgattaatt gctgggtctg cccattcata
     5461 aaaacaaaac tcatttttcc actgcaggac ttcatcgatt tgaactcatc aaaacaaagg
     5521 accgaaggta acgtatcaaa acgaggatga tacgttttta aacaggtgtg caggacacgc
     5581 agaacggtta cgtcagagac aaaatagcga tcggcaattt cttttcttgt tgtattcttc
     5641 gccagctcca aggcaatttg tacctttaat tcctttgaga gatagcagtt ttcatctacc
     5701 aaattggtct gagcgttgaa tgtcgactga cattctttac agtaaaagcg cgtacgtacc
     5761 aagcgtaatt ctgttttggt tgctcgataa ggcaaaagtt ggacttttgt tttatgtgag
     5821 ccattcttga tgatttgtcc ttcatttttg atgccacatt tacggcaatg gcttagtata
     5881 tagctaagtt tagcatggat aagattggtg cgaaaaccat cttcttctac tgtctctaac
     5941 cattcttctt cgaagaataa attttcatct gttaaaccta acaattttct agtacaatga
     6001 tacatggaga tatcctttct tggtatggtt ttgttttaga cgactaaatt ccaccagtta
     6061 ggctatctct ttttctatct ttttttacaa aaaaagtgtc agtagaaaat ctgtagattt
     6121 tccaccaaca ctaaatattg tagaatcaaa aatcagaact acatccagta gttctgattt
     6181 ttg
//
LOCUS       DANKHK010000112         6061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000112 DANKHK010000000
VERSION     DANKHK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            190..546
                     /locus_tag="PW109_002281"
     CDS             190..546
                     /locus_tag="PW109_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2512072.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="PW109_002282"
     CDS             556..1455
                     /locus_tag="PW109_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2512073.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1971..2702
                     /locus_tag="PW109_002283"
     CDS             1971..2702
                     /locus_tag="PW109_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2512074.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2847..4874
                     /locus_tag="PW109_002284"
     CDS             2847..4874
                     /locus_tag="PW109_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2512075.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            4982..>6061
                     /locus_tag="PW109_002285"
     CDS             4982..>6061
                     /locus_tag="PW109_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2512076.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKK"
BASE COUNT         2092 a         1006 c         1252 g         1711 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttgaacgat aatcacaaaa aaaatataat atacattttt tatttaatta
     1681 taattaatat tgattgaagt ctaggcagtc aagtcacatt ttgatagata ggagggggac
     1741 ttacgaatta ttttaaaggc tatgacagct gtatttctag attgtgtttg catcaagttg
     1801 caataaaaac tagaaatcaa atcgggctga taatcattta taactaatcg atacatagga
     1861 ccaaatatag gtctatgttc ttttatttca taagtatcgt aaaacagaat tttgctatgg
     1921 attaatccaa tcgactgata tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa
     1981 aagaaatcgt attaacagca gcaataatgt tattcagcat ggtagcaagt acgacttttg
     2041 tttctgcaac tgaagtatac ccaaaggaat ataacacaga aggaacgatt acatttgaag
     2101 caggagatga gggtgtaact cctcctgttg atccagagaa tcctgatccc aataaacctg
     2161 tcgatccatc agatcctcca agccctggaa caggaggggc attatcgatt gattatggat
     2221 caaaattcaa atttgggact caaaaaattt ctactgcgga taaaacgtat tatgcagctg
     2281 ctgatgtgat gaacgatggg tctagaaaac cgacatatgt ccaagtgaca gataggagaa
     2341 gtaccttatc tgggtggaaa ttaagtgtct ctcaaccaga acagtttaaa actgccagtg
     2401 gggatgaact agttggagca cagctgaaat ttacgaaagg gcaggctgtt tctttggttg
     2461 atccaacata tacgccgcaa acggtgaatt ctgaattaac gttgacaccg ggaggaaaca
     2521 atacgttagc aattaatgct aaatcaggaa caggtgtggg gacttgggtc tatcgatttg
     2581 gtgcaaatga aaatgaaaat caagatgcgg tacagctttc ggtacccggt aaatctgtca
     2641 agttagcgca acaatattcc actaaattag tttggacatt agaagataca cctaacaact
     2701 agtttactga aaagaactgt taaaaataaa tacgtagtca tattgtcttg cgaggtaatc
     2761 gagcaaaaat tgtcaaaggc taggctaaat ttgtttggat acgaatagtt catctaggaa
     2821 tttaacgatc aggaggaaaa gatatattga aaaagaggag gatcgtgctc attttaagca
     2881 gtctcttagt gactaatatc tttccgatca tagggtactc tttggaattg aatcagttgg
     2941 agcatggaaa tagaagagac gaagaaagca aactcacttc gacattcgac agtttaatag
     3001 aatccaacca gacacaagaa agcagtgagg atttaagtat ttcgagtagt ggagagacca
     3061 ctgacaacgt agaagctgag ggttcggaca aacaaggact agaagatgca ccaatttctg
     3121 cggttgagct gcctgacgat cccaatatta tttctatcga taaaattttt caaacaccta
     3181 tcggtactag cacgtccata ttggaaaatg ggaagttact gcagttgaac ccagcagaaa
     3241 ggtctcaaag aggagctatt tggtcaaata agaagattag tttactctcg gattttacat
     3301 ttaaatctta tttatactta ggaaatcaac gagatagagc gggagatggc atgaccttta
     3361 cattgactgg cgatcctaga atggctagta atcccagtca agttattgga tcgccaggta
     3421 tgggaattgg ctcatattct actcgttctg gccaacctta cattgcgaat gcgctctcga
     3481 ttgagtttga tacgtataaa aatagtggtt cttctgatcg aatggatcga gagataactg
     3541 ctgataatag agggcatgga catgtcgctt ttgtcacgcc taaaccaaat aataataatt
     3601 attctggaga gcatactggc gtgacggtag ctcctacatt tctttctaat ggcacatggc
     3661 gcttgttaat aattaaatgg gacgcggagc aaagaagtct tacttatgat ttggaaggtg
     3721 tcggtcgctc ttctcggatt atacctgatc tcattaggca gtttggttct acagaagtaa
     3781 attgggggtt tacttcttca acaggtactt tttttcaaga aaatgctgtc gctgtcacgc
     3841 gacttccatc gaatgtcaca tcaacagctg atgtaaaagt caacgatagt gattatgggg
     3901 ccatcgctga agctggagga aacgataaga taactatcag aaatacattg aaggtcgagg
     3961 gggattttct tgaatataag aaaatgcaga ctagtatcga cctcccacaa gaattaacta
     4021 ttcccgagca tatcttgatc aatggaaatc aaattgatgc caaagatatc ttgataaccg
     4081 gaaatcgagt cacgctaaat ctgacagatt attttattgc tggagatacg ttaataatcg
     4141 aattggagac aactttgcag gaagtaattc ctgaaaaagt actcactatg cattttgaat
     4201 accttgaaaa cgggaatttg attgagcaat cgaatgaaat aaacctaaag atcgcaaagc
     4261 tgaaggagat agatatccat gtttattata aagataaaga cagtcagatt gatattgctg
     4321 aaatgaaaac aatcactggg aaaataggag agaagtataa agaaactcct attgatattg
     4381 aagggtatgt ttttgactcg gattcagcga atacagaagg catcttctcc gaaaatacag
     4441 aggatatcta tttttattac agaaaaggca aactttatct ctcaactgct cctcaatatg
     4501 tcaattttgg aaaacataaa atatccagta gaccattaag tgaattcgga cagttttcag
     4561 gtgggttgaa aattatagat gaacgggctt ttggaagttg gaaattacaa ttgaaacaag
     4621 agaaagttct tacaaatgga gattttgagc ttccagaagc tctttccttt gttacctctg
     4681 aaaatacaac ggttataggg acatccgctg ttacgatttt cgaaagtgat caaaagggtg
     4741 aaagcgattt gtcctctctt ttagattcta cagctcatcg tggaatcaga atagatattc
     4801 ctgtagaaaa ccaaagaatc ggaacctttg aggggaaact atcttggata cttgcagatg
     4861 tcccaggaaa ttaacaggaa gagaagaata taacaaaatt attgagtttc aagaagggca
     4921 caggtaccat aggctcttgt gtcccacaga gacaagattt tgttatagtc ttctcttaca
     4981 tatgattaaa ggagcgattt tcttgagacc aaaacaacaa ttatttataa cactcctagt
     5041 tattttaggg gcacttttta acaatatatc agtgtcagct agtgcattta atttttcagt
     5101 gacgccgata acttcagaga atcaaatcga caagagaaaa acttacttcg atcttcaact
     5161 tgatccggat caggaagtag aagtaaaagc ggaattgaga aatgatacag aaaaagaagt
     5221 gaaaatcgat atttctgtga atagcgcaac caccaattca aatgtgatgg ttgaatacgg
     5281 aaaaaacgag attgaaaaag acatgagcct gatctttgat ctggtagatt atgtgagcta
     5341 tccacagata gtgacgctaa aacctaaaag tgtccaaacc gttgcttttc atgtcaggat
     5401 gccgaatgag cgatttgatg gtgtattggc tggtgggatc acattccaag aaatccagac
     5461 ggaaaaggac caaacagaaa ctaaggatca aagcttatcg attgagaatg aatacaccta
     5521 tattattgct ttgttgatgc aacagacact gaacgaagtt gctccgaacc ttactcttca
     5581 tgaagttaaa ccagatcaaa tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga
     5641 tcaaaaaacc tatatcaatc aagtagttat tgaaacaaaa attacaaaaa aaggacattc
     5701 tgaagtttta tatcaagagg aaaaagaagg attgcagatt gcgccgaata caaatttttc
     5761 ttttccaact gctttaaatg gtcaaccgct aaccccaggt gaatatcacc ttacaatgac
     5821 tgttttagga aatgaaaatg aaaatggaaa attttcgaga aaaaaaggaa atactacgat
     5881 caactatacc aatcaatggg tatttgaaaa agattttaca atagacggaa aagtagccaa
     5941 ggaattgaac acgacggatg ttacgattaa aaaagacaat aattggattt atatcttagt
     6001 agcttcactt ttcttattgt ttattttatt atgcatactt ttgtttgttt tgagaaaaaa
     6061 a
//
LOCUS       DANKHK010000113         6020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000113 DANKHK010000000
VERSION     DANKHK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            123..1031
                     /locus_tag="PW109_002286"
     CDS             123..1031
                     /locus_tag="PW109_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HDL2512077.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(1122..2375)
                     /locus_tag="PW109_002287"
     CDS             complement(1122..2375)
                     /locus_tag="PW109_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2512078.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            2492..3841
                     /gene="gdhA"
                     /locus_tag="PW109_002288"
     CDS             2492..3841
                     /gene="gdhA"
                     /locus_tag="PW109_002288"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2512079.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            3953..5299
                     /locus_tag="PW109_002289"
     CDS             3953..5299
                     /locus_tag="PW109_002289"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2512080.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(5607..5993)
                     /locus_tag="PW109_002290"
     CDS             complement(5607..5993)
                     /locus_tag="PW109_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2512081.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         1950 a         1124 c         1195 g         1751 t
ORIGIN      
        1 taaatatcta tacccgtggt gctagttaat tttttcttag aggaaaatgt ctaaacatgt
       61 tggtaaatca acaaaaaaac gcatatagta gtctcaaaac tcccaagaaa tgaggactac
      121 atatgcgttc atcaaaatat actgaacatt atacagatac ttttataaca ttcaaggaaa
      181 ttatgagaac tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa
      241 gaaatactga tcaactgaag caacgcgata cagtgattat tgcttgtgtt atatggggca
      301 taattaatgg ctatactagc caaagagcca cgtatagagc ggtttgttcc gtcttatttc
      361 ctaatggtga ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca
      421 ctatcaagat tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa
      481 ttatagatag ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat
      541 tgaatcaaat agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta
      601 aaattcatat gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag
      661 gtgtccatga cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc
      721 taggggacaa aggatatatt agccataaaa tccatgaaaa gcttgccctt aagggcatta
      781 ctatatccgt tccgccacgt aaaaacatgg ataaatcaga aaaactagac cacagcttac
      841 ttggaaaaca aagaaaaaca gttgagactg ttttttcttc cttagaaaaa ttaggttgtc
      901 aaaatttcaa ctcacgttct gttaaagggc tagagagtag atttgaaagc atattactgg
      961 cttacagtgt tctattaagt cgagcacaac gacgctttga aggaactttg agatattctt
     1021 taggatatta aaattaacta gcaccacggg tatcatacgt ataaaagaaa gaaggaggcc
     1081 gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca
     1141 ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa
     1201 ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat
     1261 gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata
     1321 ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt
     1381 tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa
     1441 actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct
     1501 ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc
     1561 agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa
     1621 cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca
     1681 gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct
     1741 tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa
     1801 agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca
     1861 gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg
     1921 aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta
     1981 gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt
     2041 tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct
     2101 tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa
     2161 aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca
     2221 ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc
     2281 aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca
     2341 gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc
     2401 atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa tgagctaaaa
     2461 tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat atgttcaagc
     2521 aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac aagcaatcga
     2581 tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata tcgaaaagaa
     2641 tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc cttggcaaga
     2701 tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact cagctatcgg
     2761 cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct tgaaattttt
     2821 ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg gaggaaaagg
     2881 cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt tttgtcaaag
     2941 tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg cgggagatat
     3001 cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat tgaaaaaata
     3061 tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga tacgtacgga
     3121 agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag aaaaagattc
     3181 atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt atgcaatcga
     3241 aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg ggtacatcta
     3301 tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca atagacaacg
     3361 tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag aatctgtttg
     3421 gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg aaatagacga
     3481 aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag gcgctaatat
     3541 gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt attgtccagg
     3601 aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc agaacgccca
     3661 gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca tggaaacgat
     3721 ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc tgcttcttgg
     3781 agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag gtttagtttg
     3841 aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg aaaaataata
     3901 aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac aaatggcaca
     3961 cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt tagggtacat
     4021 gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg caggaagcga
     4081 cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg cacgtatcaa
     4141 agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg gtatcggtgg
     4201 ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct tcaatttatt
     4261 agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa tcagctcaac
     4321 atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa acgttatttc
     4381 aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag agcttcttgt
     4441 taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag ataaagcaaa
     4501 aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga ttcctgatga
     4561 catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg ctgttagtgg
     4621 tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat actcaagcga
     4681 caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc tttaccgtaa
     4741 aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact tctcagaatg
     4801 gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct atccttcaag
     4861 cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag gacgtcgcaa
     4921 tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa ttcctgaaca
     4981 agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact tcgtaaatac
     5041 aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta acttgctagt
     5101 caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct ttgaaattgc
     5161 tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag gtgtagaagc
     5221 ttacaagaaa aacatgttcg cacttctagg caaaccgggc tttgaagatc ttgctaaaga
     5281 attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc agtgatagaa
     5341 acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat actgcatcgt
     5401 ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt gtatagaaat
     5461 cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc
     5521 gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga
     5581 gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag
     5641 ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac
     5701 tgcaagatcg ttccttcggt gtaagcagaa ccattggcag agtcaccaga agtaatgaca
     5761 tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa
     5821 cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct
     5881 ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc
     5941 catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt catgaaagat
     6001 ctcctttttt atttttatta
//
LOCUS       DANKHK010000114         5968 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000114 DANKHK010000000
VERSION     DANKHK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            64..1395
                     /locus_tag="PW109_002291"
     CDS             64..1395
                     /locus_tag="PW109_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2512082.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1361..1711
                     /locus_tag="PW109_002292"
     CDS             1361..1711
                     /locus_tag="PW109_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512083.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1803..2222)
                     /locus_tag="PW109_002293"
     CDS             complement(1803..2222)
                     /locus_tag="PW109_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2512084.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2223..2567)
                     /locus_tag="PW109_002294"
     CDS             complement(2223..2567)
                     /locus_tag="PW109_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2512085.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2917..3513
                     /locus_tag="PW109_002295"
     CDS             2917..3513
                     /locus_tag="PW109_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2512086.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            3637..5436
                     /locus_tag="PW109_002296"
     CDS             3637..5436
                     /locus_tag="PW109_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2512087.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(5714..5926)
                     /locus_tag="PW109_002297"
     CDS             complement(5714..5926)
                     /locus_tag="PW109_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512088.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
BASE COUNT         1608 a         1096 c         1086 g         2178 t
ORIGIN      
        1 tgatagttgt ccaaatcttt catttcaagt tatagttaaa gaaaatgaaa aagaaggtgt
       61 gtaatggaaa acgaaactga cgttatttat attcatcctc aaaaacgaat cgtttcacag
      121 aaaagaaagt atttttatct tggttttaca ggtgtttttt ttctttttat tggcttgctt
      181 tctaatactc ctactgataa ttggagtgga ttgctaacta ttcttacctc gccaagcaat
      241 cttttgacag attattttgc tttaggtgtt ttcggaagcg cattcataaa tgtagggatt
      301 ttgaccttac tttctgtgct tttagcttat cggcacaagg tgattttaaa cggtccctta
      361 tttgcttcga ttctaacagt taccggtttc tctttttttg gcaaaaacct ttataattct
      421 atctcaatca ttttaggtgt gtatctatac gctgtatttg tcaataaacc tttttctcag
      481 tacatcatga tcggactgtt tggtagcgta ttatcaccgg tcgttagtta tatcactttt
      541 ggtatgcgtt ttcctttact gataggtatt ttattgggga atttagctgg catagcaatt
      601 ggtatacttc ttccgccctt agctgctcaa actttagttt tccatcgtgg attcacactt
      661 tataatattg gattcacctc tgggttgatt gcgatgactt ttactgctgt tttgaggtta
      721 ttcagctatt caatcgtcga aaatacattg gttttcaatg aatatcactt tcctctcatt
      781 tggattattt ttggcttttt ttcgctgaca gtcggaattg gtttctatta taattccttt
      841 cggctttcgg ggattcgaga aattttcgat tcatctggaa aattgacaac tgatttcatt
      901 gctaattctg gtattggtgc tactttgatc aacatgggct tagttggtct gatgctgtct
      961 tcttatgttt tgctggttgg cggtcagtta aatggacctg tcatcggtgc tattttatct
     1021 gcggtgggtt tcagtgcttt tggttgtcat ttaaaaaaca gttttccgat actagtcgga
     1081 atatttattg cttctttgtt tggaactttt catgagatca ccagtactgg catgcttgtt
     1141 gcagctgtgt ttgggacagg tcttgcgccc ataagcggtt tttacggcag tttttacggt
     1201 gtaatagctg gtgtgctaca tattgctctt gtccataacg tcagcacttt gcacggagga
     1261 ctaaatctat ataacagtgg tttttcaact ggtttcgttg caggtatcct tgtaccgata
     1321 ctagataact ttaccgctgt cagaaaggaa aaaaagacac ttggaaaaag aattatcaaa
     1381 aagaatcatc gatgagcttc ttacttattt atattcccat gaaataactg aaatacgtat
     1441 tggagtagaa tttgctgagg aagggctttt catagagata cagggtacgg cagaaactca
     1501 gccagaagat ttagatgtac ttgctgattt gctgaacaat cctcgggacc ctagtatgga
     1561 aatctactat caagaactat taggtaatcc ccgacatgat cgggaagatt atcatctgtt
     1621 aggtagtttg attgatgacg tggaaatcat gtatgcccag ccgattatca atattaagat
     1681 ctttcgacgt gcttctcatt cctattatta accctatttt ctatttataa aagactgaat
     1741 caaaaaagca gctcctgaaa gttcgattaa aatctacttt caggagctgt tcaagcttga
     1801 cattattgat tttccaatcc ttcatacact tcatctgtta caggttctaa aaactctgaa
     1861 ttaccagcag tgatcgccaa atgttcaaac caactgtctt ttgtcgcacc atgccaatgt
     1921 ttaatccctt tgtgcgttac gacgacatct ccagccatta atcgtcttgc tggctttcct
     1981 tcttcctgat accagccttc tcctccggtc acaagtaaga tttgatagcc gtctgtatgg
     2041 acatgccagt gattgcgaca acctggttca aaagttacat tacctacacc aacagaaact
     2101 tcgggatcat tcactaacat attcagatag ctttgcccgg taaatacttg agcgtaagct
     2161 tcgttttttt cacctgctgg aaaaatcacg ccattgtgta tttcttcgtg ttttgtcatc
     2221 atttacgcct ctttcttatc ttcgctattt tcttctaaat agatttcttt tgctaaacta
     2281 aaagctgacc aagcttttgg ccattctaca taaaaagcta aatgggtaat gatttctgct
     2341 atttcttcac tagtaatgcc attttgcttg ccaatcttca gatgagctgg caattgttca
     2401 aaatttcctc ctgaaattaa tgcagatatg gtgatgatac tacgctgatg agcagataat
     2461 tcttctctgg accatacttg tccaaataaa acatcatcat ttaattctgc aaattttggt
     2521 gcaaaatctc ctaattgatc tcgtcctgcg gtttgttttt ctgccatttc tcttattctc
     2581 ccttcacgac tgttaggtga ttcaactatg gttctaaggc aactttattt tctattgttt
     2641 cttcgcattt cattttaaat gtagtctttc agcctgtcca atgcttagtt tattcagtcg
     2701 atatgctttt taagcatagt tgctaacgct ttttttattt taaaaaaaac aagttattaa
     2761 ataagtcttt agttggatgt ttttttgaga tcaatctttt atacttatga tgaacttaca
     2821 aaattgtttg ttttctgtca tatcaaaaaa tggcgcagtt agttgctttt ctagtaaagt
     2881 atagttaaca aagaacctct aaggagttga tgaataatga atattgctgc ctctcgaaaa
     2941 ctgataaatt tcatatccat tatcggatta ggcctatcaa tcgcgctgac gatttatttt
     3001 attaatttag gtgttttcaa agatttgaat gcgttaagag gattagtagg tgattccatc
     3061 attttaggac cgattatttt tgtcttcatc caaatcctgc aagtagttat tccaatcatt
     3121 cctggaggga tcagtactgc agctggtgtc ctgatatttg gcccttatgc tggatttatc
     3181 tataactata ttggaatctg cattggttcg attatcattt ttctgctagg gcgtcgttat
     3241 ggaaagccat tcatactttc catgattagc gataagacat ataacaaata tattggctgg
     3301 ctggataatc aaaaccgttt tgagaagctg tttgctctag ctatttttct tccagttgct
     3361 cctgacgatg cgttatgttt gatggctgga ttgacaaaca tgtcagtgaa acgatataca
     3421 ctaatcattt tgattgctaa accactgtcg attttcttat atagtatggc cttgatttat
     3481 ggaggtcaat atctaagtgg tcttcttgga taatatttat tttgtaaaat tagtcattga
     3541 ttgacaatgt taaatctggg aaagggtctg caaaacacac tgcacccttt cctttttcat
     3601 tagatttgcg ctacaatgtg aagagcaggt gataggatga attatttgaa acgtagctta
     3661 ggtaatgctt gggacttttt aaaaggaaca caagcttatt tccgtgatgt cttgctgatg
     3721 catggcttta ttttatttat ttgtttaccc ttattgagca gtacgacccg ttttatttta
     3781 caccgagggg caattgacta tttgtcttat gacaatattc cttcaatttt ttctcaacat
     3841 cctggggttc tactctcgtt aatcgtagtt ttgatgacga ttttactgtt agtttatttc
     3901 gaatttactt ttcttttgat gagtgttttt tttattaaaa agaaagaacc aatctctttg
     3961 aagcaactct tacacttgac gatacttcag ctgaagaaag tgagacccat tacttttttg
     4021 tttttccttg cttatttttt attgattcta ccgatcagcg gattaagttt taattctgat
     4081 ctactgtcaa aaataaaaat ccccgctttc attatggatt ttatttttgc taatcgttgg
     4141 atcatcgtca gttccttttt actcgtttat attttccttg gctatattgg gattcgactg
     4201 atttttgctt taccggaaat gattctacga gatcgttctt ttcgagcagc cattcgggaa
     4261 agctggtcat tgaccaaatc gcgtcttttg gccatcacgg gccaatttat tgtgattggc
     4321 ggtacgattt tactcctttc ttctttagga tatatcgttg ttattttggc acaaagtatg
     4381 gttgaacaat ttttcccaga ctatgcattg atcagcgctg tgtttgctat gacgctttta
     4441 caagggattt tattattcaa tatcgtgatg tctaccgtag ggatttttta tatcattgtt
     4501 gatttcatgg atgacgaagg cttcttgcct gagatcccta agtggtttat cccacaagcc
     4561 cccaacttgc gtttctctgc gctgaaaaac actggtttga ctctttttgc agtttttttc
     4621 ggtattggcg tctgtcttta caatatggat tatctgactt cagctgttca gacaaaacca
     4681 gtgactgtct ctcacagagg tgtgagtacc caaaatgggg cgcaaaatac tttagaagca
     4741 cttgaaaaaa caagcagaga ctatcatcct gattatgtag agatggatgt tcaggaaaca
     4801 aaagatggtc atttcgtagt gatgcatgat gcaaatcttc gtcacttaac aggggtaaat
     4861 ggaacaccac aggatcttac attgaaagaa ttgacaaatc ttacagttac tgaaaacgga
     4921 gtgcaagccc ctgtttgttc ttttgattct tatttggaaa aagcacataa gctaaaccaa
     4981 aaattactta ttgagatcaa gacctctaaa aaagattcgg atgaccttgt cgaacgattt
     5041 gtccataaat accgcaaagt tatcttagag aaccatcata ttttgcaaag tttgacgtac
     5101 caaaccgttt cagatttaaa agaagcagaa ccaaactttt atgtgggcta catcttacct
     5161 ttcaatattg ttgggccccc tgtgacgcct gctgattttc tgacaatgga atatagtacg
     5221 atcaaccgta acttcattaa ttctgcccac caagacggaa aagaagtata tgtatggaca
     5281 atcaacgatt cagatgccat gagtcgcatg atgttttatg gtgtggatgg tatcataacc
     5341 gatgagatgg gactactaaa tgaaagtgtg aaacaaacag atgaagaaat cacttattca
     5401 gataaactgc taaattttgt tttgggagtc ggctaatcaa aaatcgttaa tgaaaaaagt
     5461 ctttatacaa caaaaaacgc ataacctgcc accgcaggta tatgcgtttt ttgtttgacg
     5521 ctcataatgt gtgcttaact ttctcttgat tccttcatcg cacgaacaat agctaaaata
     5581 gcaggaattg cagagatcac tgcaagaata attcccattt tccctgaact ttttcttttt
     5641 ggcatatctt ctcgctccct tctgtcttta tttatattat aagggagttc ttgcgaattc
     5701 tcaaaaatac tgtttaagaa accaattcag taggtgcgct aatatcacag aattttacga
     5761 gcatgcgata cttgctttca ttgataagtt gatagtctcg ttggtcataa ctttcaacat
     5821 acacgacagc cgtgttgata tatgtacgtt caatacagcc gattagttca tgagatactc
     5881 cgatcggttt gcatatataa gatcttccta cttctgcttt taccatttca ttccctcttt
     5941 tctttatttt tattttgttt tttcttta
//
LOCUS       DANKHK010000115         5729 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000115 DANKHK010000000
VERSION     DANKHK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            19..103
                     /locus_tag="PW109_002298"
     tRNA            19..103
                     /locus_tag="PW109_002298"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Leu,seq:caa)
     gene            165..237
                     /locus_tag="PW109_002299"
     tRNA            165..237
                     /locus_tag="PW109_002299"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:His,seq:gtg)
     gene            247..318
                     /locus_tag="PW109_002300"
     tRNA            247..318
                     /locus_tag="PW109_002300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Gln,seq:ttg)
     gene            336..420
                     /locus_tag="PW109_002301"
     tRNA            336..420
                     /locus_tag="PW109_002301"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:370..372,aa:Leu,seq:caa)
     gene            675..1550
                     /locus_tag="PW109_002302"
     CDS             675..1550
                     /locus_tag="PW109_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2512089.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1654..2418
                     /locus_tag="PW109_002303"
     CDS             1654..2418
                     /locus_tag="PW109_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2512090.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2405..3847
                     /locus_tag="PW109_002304"
     CDS             2405..3847
                     /locus_tag="PW109_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2512091.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTVFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3858..5189
                     /locus_tag="PW109_002305"
     CDS             3858..5189
                     /locus_tag="PW109_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2512092.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5202..5660
                     /locus_tag="PW109_002306"
     CDS             5202..5660
                     /locus_tag="PW109_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2512093.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1893 a          871 c         1227 g         1738 t
ORIGIN      
        1 gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc
       61 agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta agatgtcttc
      121 atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat gtggcgaagt
      181 ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc attcgccctt
      241 tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
      301 cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa ttggcagacg
      361 cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg gccgccggta
      421 taattatcaa tatagcaact ttcttataga gagttgctat attttttttg tttttatgta
      481 attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa agatattgat
      541 aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta ctattcaatt
      601 aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa aattgtttgg
      661 agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat agaggaggtg
      721 aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc gatgacgatg
      781 gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg agtaagccat
      841 ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag atcaaacgat
      901 tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa gaagtgattg
      961 aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt acattgacag
     1021 atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca gcaattatgt
     1081 tgccagaaat gagattcctt tatccaaatg aatatgatat agcagtagaa gtggtcgagc
     1141 gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc atcactatgc
     1201 atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa atagtagaaa
     1261 tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat gataaagaat
     1321 cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc ttgatttatc
     1381 aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa catagcaaag
     1441 cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa cttgccattc
     1501 aagaaaatga aatcatctat ttggcaattc atctagcaag aatctattaa aataagggcg
     1561 taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt atttggttgc
     1621 gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt taattcggat
     1681 gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca gcgaggaatt
     1741 ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcatgctgt gaaactaaga
     1801 aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg caaaccactc
     1861 ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc ttattatcag
     1921 catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca gattcaaaaa
     1981 atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac tcatactttg
     2041 gatgaaaatc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc ggttcggagc
     2101 aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg tttcgatgtc
     2161 gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga atatttggca
     2221 agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca cgcagcgtat
     2281 ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtaccca ccaagtcgaa
     2341 tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat tgagttagta
     2401 acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa atgtgcagaa
     2461 gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat tgatcatgcg
     2521 tttatcagct gacgatatgc tgaacatccc agtaattggt gcagctggaa atgccgtttt
     2581 tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca aaaataagga
     2641 caaaggaata cctgcactta ctgttttttt agcgattgct accttgaaaa aaggcttaga
     2701 aattatggac ccagaagtga atatggggat ttttggtgga attatttccg gtttagtagc
     2761 ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt cttattttgc
     2821 gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag ctgttttttt
     2881 gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac tgctcgttga
     2941 tatgggagca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga tcccctttgg
     3001 actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata catctcctag
     3061 caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc aggctggact
     3121 gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat gtctagctat
     3181 ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg gaagcggtgc
     3241 aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca tgtttctttc
     3301 tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg tctgttactt
     3361 attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc tcatcaattt
     3421 tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt ttgccttgta
     3481 ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag gacgtgaagc
     3541 taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt tagcaagtaa
     3601 aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag ccaatatcct
     3661 ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca ccatggtcga
     3721 ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg aaaaaaatta
     3781 tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata aattattaga
     3841 agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct ggaggcggga
     3901 gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg ttgccattat
     3961 ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac ttgattgtta
     4021 aatttatgct gaaaagaaaa ggtttttcaa atattcgcat tcgtgcaacg gatgatccaa
     4081 agcttgcttt tacaggatgc gattttgttt tctcacaaat tcgtgttgga ggattggaga
     4141 tgcgagaaaa ggacgaaaag attccactta aacatggatt agtaggacaa gaaacgtgtg
     4201 gactcggagg atttgcttat gggatgcgct caatcaaagg tttattggaa attgtcgatc
     4261 atatacaaga ctatgccccc aaagcatgga ttttgaatta tacaaatcca gagtcgatcg
     4321 tttctgaagc tgttcgtcgt aaatatccga atgtaaaaat gattaatgcc tgtgatatga
     4381 ctatttcaat tgaagaaacg attgcagtga attacggtta tgatcgtaag aattggatcg
     4441 ttacgtatta tggtttaaac cactttggct ggtatacatc gatttatgat aaagaattgc
     4501 aacgagaaat tatgccagag attattgaaa aactaataac aaaagaaatg caagtcgccg
     4561 attttaatat aggagataaa acttggcaaa aaacctttca aatgatgtca gtcatcacga
     4621 aaaattttcc aagtaatatc ccgaataatt acttagagta ttacttatat cctgatatgg
     4681 tcgttgagca tactgacaaa gagtatacac gtgctaatat ggtcatggat ggacgagaga
     4741 aaaataccaa agaaatggct gataaaatac gccgcggtat tgtggaagaa gtattgaact
     4801 ttaattttgg ggagcatgga caatatatcg tcgatatagc tatttccttg ttgaatgatg
     4861 atcgtcgtcg ctttatgcta atcgttccta atcaaggggc gattccgaat ctacggtcag
     4921 atgcagttgt tgaaataccg gcatatgttg gtgcaactgg tgtggagcca attacactaa
     4981 gaaaaccaat cagtgatttt cataaaggat tgatggaggc acaagttgca gtagagaagt
     5041 tactagtaga tgcttacttt gaaggttctt atcaaaaagc tttacaagca tttacattga
     5101 atcaaaccgt accaaatgcc agagtcgcaa aaaaaatact agatgaaatg atcaaagtaa
     5161 acaaaaaata ttggccagca ttaaagtaga ggtgataaaa agtgcatttc tttaaaacaa
     5221 gaaacattct aaaagctgta gctagtggtc aaattattcc attaacagca gtaaaagacg
     5281 aggtcttttc ttcaaaaatg ataggagttg gatacgctat aacgaatcat gatggatata
     5341 tccatgcgcc agttaaagga ataatcgata atatatttcc tacccgacat gccataacga
     5401 tcgaaagtga atctggcaga aaaatactga tccatatggg tttagataca gttgaactcg
     5461 aaggaagtcc gtttcagata cgggttgaaa gtaaccagga ggtaaaagaa gggacactgt
     5521 tagctattat agatttgaag aagttggagc aagcacaaaa agatacggtg atcattgtag
     5581 tttctccagg catttttgaa ggaaatttat taaaacaaaa ccaagaagta aagattaaag
     5641 atagttgttt tgaattttag tgtacgatga aaaaagttaa atagggtgat atttatgggt
     5701 gaatttacaa aatttttttg aaatgatat
//
LOCUS       DANKHK010000116         5725 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000116 DANKHK010000000
VERSION     DANKHK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            17..688
                     /locus_tag="PW109_002307"
     CDS             17..688
                     /locus_tag="PW109_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2512094.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            747..1187
                     /locus_tag="PW109_002308"
     CDS             747..1187
                     /locus_tag="PW109_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512095.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQITNKKQ
                     AKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1199..1867
                     /locus_tag="PW109_002309"
     CDS             1199..1867
                     /locus_tag="PW109_002309"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2512096.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYASVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKKWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1931..2554
                     /locus_tag="PW109_002310"
     CDS             1931..2554
                     /locus_tag="PW109_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2512097.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAKEFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2610..2996
                     /locus_tag="PW109_002311"
     CDS             2610..2996
                     /locus_tag="PW109_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2512098.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3209..4600
                     /locus_tag="PW109_002312"
     CDS             3209..4600
                     /locus_tag="PW109_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2512099.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4812..5270
                     /gene="tnpA"
                     /locus_tag="PW109_002313"
                     /pseudo
     CDS             4812..5270
                     /gene="tnpA"
                     /locus_tag="PW109_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1917 a          942 c         1203 g         1663 t
ORIGIN      
        1 aaaaaggggg agaaaaatga attttttagt tgttttaaaa gatactctga ttgagcgatc
       61 tacatttcta tatgttcaat tgcaggaaaa taaaacattg ctgcattttt caccagagtt
      121 ccatctagag gggctaacat taggagaaat ctatcaagca gaagggctgt cggctgtttt
      181 gcactttttt aaggtagaat tggaattacc agtaaaagat tatatcgagc tttctcttga
      241 atcttgggat cgagcagtgg ttgaactgtt tccagaaggt ttaatgatcg atacaaacga
      301 tgggaagatc catctgactg cagcagaact gcaaaaacaa atgcgttatc aaatagatga
      361 cgaaaattct ttttctattt ttagacgtca gcaaaagatt ctaaaaagct ttttaaaagt
      421 gatcagtaaa aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct
      481 gcttcacacg tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat
      541 gcaacagcta ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa
      601 cagagaagat aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca
      661 ccaagttgca atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat
      721 aaatattaga tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat
      781 ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc
      841 gcggtctatt gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa
      901 tcggacaatt gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa
      961 aaatctaccc cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag
     1021 aaaaatatca tcagataaca aacaaaaagc aagctaaaat ggatcgaaaa agttatactt
     1081 tttttgagtg cccgaattgt catcaaaagc agcgtgcgcc taaaggaaaa ggacgaatac
     1141 gcgtgacatg caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat
     1201 gaaattagta ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg
     1261 atggctagat gtcgccttaa cagaaaaagg aaaagaacaa gcagcagtag ccggtcaaaa
     1321 aatgcttgaa gctggccttc aattcgatgc cgtttatgcc tctgttttga aacgtgcgat
     1381 tcttaccgga caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac
     1441 atggcgtcta aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa
     1501 agaaatatac ggaaaagatc aagtaaaaaa atggcgcaga ggatatacgg aagttccgcc
     1561 gttaaccgca gaaaatagat ttgaccgcag atatgatcac ttggacccgc atttaatccc
     1621 atacggagaa agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct
     1681 agcaccaaaa ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg
     1741 agcactgaca aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc
     1801 aaatgcacaa ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat
     1861 actttaaaga acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa
     1921 caaaaggaac atgaaaaatt atatatggga ctttgatggt acattgtttg atacgtatcc
     1981 cgcaatggta gatggtgcat ggcaagcact aaaagatttt ggtatcagta tggacaaaaa
     2041 agaaatctac ttcaaaatga aaaagtattc tactagttat ttgatcaatg aatcaaactt
     2101 gaatgccaaa gagtttaatg aattatttca ccgatatgaa aaagaaagta cggaagtttc
     2161 ccgtccattt ccagaaacga aacaagtact ggaaatgcta aaagacaacg gcggtcgtca
     2221 tttcatcctg acccaccgtt tgacagaatc aacatggggc ctgttaaaag aacatcgatt
     2281 ggcacatctg attgaggaag tagtagggat cgatcaggac tttcctcgta aaccggatcc
     2341 agcatcttta aattatttga tcgatacttt tcatttagaa agaacagaca cgatgatgat
     2401 cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta
     2461 cgacatcgat cattttctgg gtgagatccc ggcagattat gtggtaggaa atctgaatga
     2521 aatccttacc ttgcagagca tcattaaaaa gtagtatccc ctttcaaagg agatatgtta
     2581 taattttcat aaaggagtga taaaattata tggaaatcag aagacaaaaa gaagttgtag
     2641 aagcatttca ttatgatatg agattgccga accaagaagt agaaacagat ttgaaagtgg
     2701 gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa agaacatagc atcgtaggag
     2761 cacgcttaga gttccgttta gtgtttgata catatgtttt atctggaagt gtgagtcaga
     2821 tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg
     2881 acgagctagt agcaccgtta ttcgatatcg tacgccgaat gtcttatgag gtgacagaga
     2941 tcgccttaga tcagcctggg gtaaaattga attttcaatc agaaaaacaa gaatagacag
     3001 taaaaacata cctgcgatag attaaagcta tcacaggtat gtttttttat tactaattgt
     3061 aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaaa
     3121 ttttttcagg aggatggttg aagaatcatt ctcgggaacc tttacttcct tcgatggact
     3181 tgtcattaat aatatgagga ggagaaaaat gacaacactc atcacagttt tagtggttct
     3241 agtttttgcg gcattattat atgtgttcta tcgtatgcaa aagcgccata caaaattttc
     3301 aacacgcgtc tttacagcat taggagttgg tatcgtcttt ggcggtctgc ttcaattgat
     3361 ctttggtgta gacgacaaag tcgttactca atccatggaa tggatcggga ttgtaggtca
     3421 aggatatgta tcattcttgc aaatgctagt tatgccttta gtgtttattt cgatcattgg
     3481 tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa attagtgcga ctgtattgac
     3541 ttctttactt ggaacaacag ctattgcagc attgatcgga attggttcaa caatgctgtt
     3601 cggactccaa ggagctaaat ttacacaagg agctgcagaa acagcacgaa tctctgaact
     3661 ggcaacacgt caggaaacag ttgaaaactt atcgattcca cagcagattt tatcctttat
     3721 ccctaaaaac gtatttgctg atttagcagg tagtcgtgca acaagtacga ttggagtcgt
     3781 tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga
     3841 agcagcattc ttttcagatt tagtcgacag tctgtataaa atcattatgc ggatcgtgac
     3901 gcttgttttg cgtttgacac cttatggagt atttgctttg atgacaaacg ttttggcaac
     3961 aagtgatttt gcagctatcg tcaatttagg taaatttgtc gttgcttctt acgtagcatt
     4021 gcttgctgtt ttgattgtcc actcactgat tttgattggt gcaaaagtca atccaatcaa
     4081 ctatttcaaa aaagcctttc cggtattgag ctttgcgttt acttcacgtt caagtgctgg
     4141 agctttgcca ttgaatatcg agactcagac aaaagcttta ggagtagatg atgcaacagc
     4201 taatttctca gcaagttttg gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc
     4261 agctatgttg gcaacgatcg tcgcaccagc agctggagtg aatgtcttta gtttggaatt
     4321 tattttgatg cttgtggcaa ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg
     4381 gggtgcaaca tttgcttcac tgatcgtttt aggggcaatg aacttgccag tagcaatcgt
     4441 tgggttagtt atctctgtcg aaccattgat cgatatggca cgtacagcag taaacgtcag
     4501 cggtagcatg gtagctggta tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt
     4561 catcaatgat gattctaaag tgatcgatgt gaatgcatag tcatagttaa aacatattag
     4621 gaaatatact aagattatag ggcaaaatca tcgatagatg attttgccct atattttttt
     4681 gtgtgttttg aaaaccacct gctatgcagg tggttccgga aagcttctag cgaggtataa
     4741 aaaaagccac ctaaactgat aagatgaggt ttcccgacca ccacatcaaa aagtttagga
     4801 ggtacctttt tatgaaaaag gctacagaaa gtttatcaca cactgtttgg aaatgtaagt
     4861 atcatatagt attcgctccg gaatttcgtc accaaatcat ttatggcaat tataaagcaa
     4921 gtattggaga gattatacgc gaattatgcg aaagaaaggg cgttgagatt ttagaagcaa
     4981 atgcttgtcc agatcatata cacatgttag tcagtatccc accaaaaata agtgtctctc
     5041 aatttttagg ttacctaaaa ggaaaaagta gtctaatgat ttttgataga catgcagaac
     5101 tcaaatatcg ttatggaaat agaaagtttt ggtgtagagg atattatgtg gacacagtag
     5161 gacgaaacaa gaaaaaaatt gcagaatata ttcgtaatca attaagagaa gaccacgtag
     5221 caaatcagct tacattgttt gaagaagttg atccgtttac tggagaaaaa accggaagaa
     5281 acactaaggg cttctttgag aagcagcgag taaatgtgga gcaagaggga accgattcgg
     5341 taagcctttc aggcttaggc cggtaaaaga ggctttcagt cgcagaacaa accacccgtt
     5401 catacgggtg gttttgatta tttttggatt atagtgagga gtgaaagaaa gttcgaacaa
     5461 aatcgtcctt cttatttacg tatgttgaga ctataagtta catgattttc ggcctaatct
     5521 aatagttttg ttcaaccatt taaacttaat tgcgtactta cctatccttt taagcttatc
     5581 agtgaagaat caaactctct atactagctt ttattttatc agataaatag ctcagaagat
     5641 catggtaggc gtataaataa ttcatgtaga gaattcactt tttatgctat cctaaaacta
     5701 ataaaaagat tatttggtct tactc
//
LOCUS       DANKHK010000117         5712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000117 DANKHK010000000
VERSION     DANKHK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(257..623)
                     /gene="ssrA"
                     /locus_tag="PW109_002314"
     tmRNA           complement(257..623)
                     /gene="ssrA"
                     /locus_tag="PW109_002314"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(794..1567)
                     /locus_tag="PW109_002315"
     CDS             complement(794..1567)
                     /locus_tag="PW109_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2512100.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1711..2061
                     /locus_tag="PW109_002316"
     CDS             1711..2061
                     /locus_tag="PW109_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2512101.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2042..2758
                     /locus_tag="PW109_002317"
     CDS             2042..2758
                     /locus_tag="PW109_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2512102.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2758..4206
                     /locus_tag="PW109_002318"
     CDS             2758..4206
                     /locus_tag="PW109_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2512103.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4276..4785)
                     /locus_tag="PW109_002319"
     CDS             complement(4276..4785)
                     /locus_tag="PW109_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512104.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4775..5500)
                     /locus_tag="PW109_002320"
     CDS             complement(4775..5500)
                     /locus_tag="PW109_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512105.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1932 a          998 c         1124 g         1658 t
ORIGIN      
        1 ccataattgt gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtgtacgaac
       61 aaaagttctt tttgtaaacc ttaaaataga ttttcctgaa tacgtattgg ggatttctaa
      121 atgtttgaat gcttttttga atgcaaaatc aatgcaaaaa attttgcatt tctctacttt
      181 tctctccttg accaaaataa aaaaacgttg atttaacaac gtttttcata catgtttatc
      241 tgtctcaacc atacatatat ggagacggcg ggagtcgaac ccgcgtccaa acacatcgcc
      301 acttaaaaat ctacgttcat agtcaactca tttaaggatt cgctttacag cttgccgagt
      361 gacaggcatt ctgtatcgct agtctgatgt tctctttttc aacttacaga cggaaagttg
      421 aaacgtatcc cactaaagtt aggaccctta ctcgagcaca tgggcgatgc cgagaggatc
      481 tacgctaact gtttttaggc agctaaagcg taagagttgt tttcgttttt agcagttata
      541 tttaactgta acgtttttac gtagacgtaa cctacgaaac gcaattcaag ctcgactgtg
      601 cctgtcgaat ccgtaacgtc cccgttagct aacaagggtt acgtgtattt ttcatacaaa
      661 attagtatag catatattgg gataaattca ataaaaaagc gaatcatatc ccattattta
      721 tataataact gcccctttct taagaaaaaa taagcgattt tcttaagaaa agaggtagct
      781 tcaaaaaaag atcttaaatc agatcaaaat acttcaatgc gtggacaatt ccgccttctg
      841 tatttttctt tgtgataaag gtagaaagtt cttttaatcc gtcataggcg ttgcccatag
      901 cgattttgtt gtcgcaagct tccaacaagg caaaatcatt cggaccatcg ccaaatccaa
      961 aagtaggaac tttttccagc tgaagttctt tttgcagacg tttgacacct gccccttttg
     1021 agattccttt cttcactgta tcgatcgagt aaggaccatt ccgataaaaa gttagctcag
     1081 gaaaatgttc gtgatagtat tcgtcacctg attgagataa gatcagcaac atatttactg
     1141 tgttttcttt aaagcctaaa ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag
     1201 cattgatcat tgtttggcta tgaccggtac accagatttt ctgatcgtta tagtaagaaa
     1261 gttcatgacc gttcaactgt acatgatcat acattttttg acattcttct gaagaaaatg
     1321 tttgagaata gatttcttcg ccgtctactc gaatgaaaga accattcatg gtgaccgcag
     1381 aagtaatgcc cgcatcttcc atgatagagt ggatttctgc ttctgtacgt cctgtagcga
     1441 tgacggggag aacattgttt tcttttaatg cgttcatagc ttttgccact tcgggtgtga
     1501 tttttgattt cgcatctagt aatgttccat caagatcaaa gaaagtgatt gcacgataat
     1561 ttgacacaga taaaactcct tttttattgg aataagttta tcttaacaaa gaatgaacaa
     1621 aagacaaacc tttcgtgaag aattcctgaa catattggtt cagtcaacaa tttagagtat
     1681 actataagaa gagaatttag gagggaaaga atgatttttt tcagtattgg tatcttattc
     1741 ttaataattg gcagtctttt tcggattctg ccctcaaaag gaaatctgcc tttttatggg
     1801 taccattcgc ctcttgccgc aaaaacagac gctcattggc ggctggctca aaagacaagc
     1861 ggaaactggt tttttcttat gggcttactg atggcattga tcggctatta tttaaagaca
     1921 tctgggcata cgaattattt tctgattgaa atgcttttgc tagtatttcc aattatgcct
     1981 atttttatta tgacagaaaa gaaattacag aaatatgacc tggaaactgg agggaacgat
     2041 aatgaatatt ttaatgattg aagataatca atctgtttct gaaatgatgc agatgttttt
     2101 cttaaatgaa ggttgggaag caactttcaa gtatgatgga aaagaagggc tcgatgcctt
     2161 tttagaaaac ccacaacatt gggatatgat tacactagac ctgaatttgc ccacactgga
     2221 cggtatgcta gtaggaagag agatccgcaa agtttcaagt actgtgccga ttatcatgct
     2281 gacagctaga gattctgaaa gtgatcaggt gatcggctta gagatggggg cagatgatta
     2341 tgtcacaaaa cctttcagtc ctctgacgct tattgctcga atgaaagctt tgcatcgtcg
     2401 tagtgagttg gcagaagcaa gagaagaagc agcagtttca gatgatcatt ttgatatcga
     2461 gactagtcat ttcaagatga atacgaaaac acgagaagcg tatctggaca accggccaat
     2521 tgaaggacta acgcctaaag agttcgatct attgtttact ttagcaaaaa aaccacgtca
     2581 agtattctct cgggagcagc tgcttgaatt agtctgggat tatcagtact ttggtgatga
     2641 acggacagtg gacgctcata tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca
     2701 agtcatccaa actgtttggg gagtcggcta caaattcgat gattctggtg ttgcctaatg
     2761 cgttatttgt atcagcaact cttagctttt tgcggaatgg tcgcgttgat cttattgatc
     2821 gttggagttt cttttacaca actgacaaaa caaacgattg aagaaaacaa ttaccagcaa
     2881 ctgtttggat atgctgaatc agttgaaaag acaacgcaaa cctatgccga tacgctcccg
     2941 cagtttagtc aagatcaggc gtttcaaaat gcattgctgc tgacagagca agtactgaca
     3001 gaacaaaatg tgaactttat atttatcgat aaaaacgaac gtgtcattta tccgactaat
     3061 acagctttac tgaattttac cttaacagct gatcaatgga aaaatctgag gaatggacgt
     3121 caagaaaaat tcacatcgaa taaaaatatt ttaggacaag cacaggcgac ctcttacgca
     3181 cttgtgccat ttaatttgaa tcatgaattc tatggagcac ttgtagttag tcaacctgcg
     3241 cgaaatatcg ataacagcgt tcgtccaatt acactgaacc tgttcaaagg atttattttt
     3301 tccagtatcg ttgcagttat tgccagctat gcttttgcag cattccaagt taagcgtatc
     3361 aatcggttaa ggaatgcgac gaaagaagtg acgaatggta attttgatgt acagctcccc
     3421 gtacatgaca aagatgaatt tgatgagtta gcagatgatt tcaataaaat gacgaattca
     3481 ttgaaagaat ctcaagcaga aattgaagaa caagaaaatc gtcgaagaca atttatggca
     3541 gatgcttctc acgagatgag aacacctctt acgacaatca acggacttct ggaagggtta
     3601 gaatacaatg caatccctga aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa
     3661 acagagcgtc tgatccgttt ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat
     3721 caaatcagta tggtcattaa aaaattcaat ggaacagaaa cattgaaaaa tattgttgca
     3781 cagttagaag ctaaagcaga agcagcagga gatacattga cattgaaagc cgttgatgat
     3841 attgatgttt acgctgatta tgaccgattt gtccaaatca tggtcaatat tattcaaaac
     3901 gcgatacagt ttactgaaaa cggccaaatc acgattactt tagaaaaagg ctatcttgaa
     3961 acgatcatca cgattgaaga tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc
     4021 tgggatcgtt attataaagt agatccttca agaaaaaata caaaatatgg tgaatcagga
     4081 ttaggtttac caatcgtgca gcaacttgta cgcttgcata aaggaaaatt agaagtagaa
     4141 agcgaactag gaaaaggaac aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa
     4201 gagtaaaaaa caagcggtat aaaagatcat gaatcgatct cctataccgc ttgtttttac
     4261 tataaaatga ttgatttagc ccgcgggctc taaacgatca atataaggac tttgagcaat
     4321 agtagcatca tgagtaaaga gtaagatacc ttgattttct tcatgagcta aataagaaag
     4381 caagtggagt agctcttgga tctccaatac actaagttca tcaaatgtat cttcaataac
     4441 aatattgttt cgcttggaaa gcaaggcatg aagtaattgc agctttactt tttcaaaaga
     4501 cgtcaactca gctgccgcac aattaagcaa ggaaggagag atatcaagtt tttcttgata
     4561 ggccaaacga tcttgtttga tttctttttc cggtacatcc aacaggacat ggtctttaag
     4621 attcagataa ggaaacatcg gccaattttt ttggatgatc gcaaaattat ccagttttgc
     4681 aacaagaacc tcttttttca agtgctccaa tattttatgc tgtttttcga gaggacacaa
     4741 aataacggtc aagtgttcag catgaatcaa atattcaggt ttcattacgt ccaggcctcc
     4801 tactttgcaa atgatggata aaaaaagtga aagggaatag actaagaatg agacagcatc
     4861 cactgagccc aataagagtt tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat
     4921 tttggaaatc gatggtaaaa aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg
     4981 aagtcaagat atcacggata tctggcagtt cagtagtctt taagacaaga aaattgatcc
     5041 tttgaagaag cgcttcataa actgactgga agctgacgat ccacaataaa accaagatac
     5101 aacaaagaaa gagaggaaag aacagctgcc agaaaataaa aaggaataat ttggttttag
     5161 aaagaccagt caggcgccaa ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa
     5221 agaaaaagat aaaaaggaag gcaaatagaa aaaataaact gaatatgcct tttatataaa
     5281 atcggctagc taattctaaa gccggaagaa gctctgtaga tgcagaagcg ccaataaatg
     5341 aaagccgatc ttggacttga ttggctaaag ccgattgcga atggtaaaga gtcaggcatg
     5401 ctatcaataa aaaagcagaa aagcttgaaa ataaacaata gagtattgtc tgctttttgt
     5461 aataaattaa tcgagcagta gcatagcgga ttattttcat acttttcacc tctataaaaa
     5521 gtatagaaaa tgaatatgaa taaagtatga aattccttta gtgaaatcac gaattaaaac
     5581 cagaaaatat tttgtaaaaa gcaagaggtt gtgaagaagc tgccctattc tattaccgta
     5641 gatgcaagct tttgaacact gtttattcga aaagaggagc agcgacaata attttgtcgc
     5701 tgctcctttt aa
//
LOCUS       DANKHK010000118         5672 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000118 DANKHK010000000
VERSION     DANKHK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            238..1629
                     /locus_tag="PW109_002321"
     CDS             238..1629
                     /locus_tag="PW109_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2512106.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="PW109_002322"
     CDS             1688..2491
                     /locus_tag="PW109_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2512107.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="PW109_002323"
     CDS             2524..3378
                     /locus_tag="PW109_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HDL2512108.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="PW109_002324"
     CDS             3375..4076
                     /locus_tag="PW109_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL2512109.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="PW109_002325"
     CDS             4084..5151
                     /locus_tag="PW109_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2512110.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAILENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..>5672
                     /locus_tag="PW109_002326"
     CDS             5144..>5672
                     /locus_tag="PW109_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2512111.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGK"
BASE COUNT         2003 a          840 c         1102 g         1727 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 atacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aa
//
LOCUS       DANKHK010000119         5613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000119 DANKHK010000000
VERSION     DANKHK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(105..1502)
                     /locus_tag="PW109_002327"
     CDS             complement(105..1502)
                     /locus_tag="PW109_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2512112.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(1534..2505)
                     /locus_tag="PW109_002328"
     CDS             complement(1534..2505)
                     /locus_tag="PW109_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2512113.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSEFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2879..3304
                     /locus_tag="PW109_002329"
     CDS             2879..3304
                     /locus_tag="PW109_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512114.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(3791..4492)
                     /locus_tag="PW109_002330"
     CDS             complement(3791..4492)
                     /locus_tag="PW109_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2512115.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(4508..4948)
                     /locus_tag="PW109_002331"
     CDS             complement(4508..4948)
                     /locus_tag="PW109_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HDL2512116.1"
                     /translation="MSQIYLAGPFFSEEQINRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(4948..5523)
                     /locus_tag="PW109_002332"
     CDS             complement(4948..5523)
                     /locus_tag="PW109_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2512117.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1607 a         1165 c          888 g         1953 t
ORIGIN      
        1 atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt tcacaaaatg
       61 gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc ctcaaatccg
      121 tttttatctg atacgtcttt gaaccagtga cccgattttt tgattgttct ttttccatct
      181 tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt ccatgaccag
      241 ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg aattgcttga
      301 tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg gatcatgcca
      361 tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga aataaagcag
      421 tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc ttgttcatag
      481 atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt gtcgaaataa
      541 tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt tacccggcgt
      601 ggctggtaat aattgatacc taaaagatct actgtatttt ctttgatgac cgtcaaatcc
      661 tctggatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa ttcacttgga
      721 aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc tgctatttgc
      781 gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt cagattcaag
      841 atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc taacgctgag
      901 gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc ttctggataa
      961 tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg agtaaaccat
     1021 ttttttactt tcgaaccgaa caattcaaaa caacttttgg catatttttc ataggcttca
     1081 accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat atcgaaatga
     1141 tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata acgatgatag
     1201 aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaagcg gctccattgg
     1261 atagatgttc gaaatgaatt gtgcccagtc tgtttcatca actgtacatc ctcttgatac
     1321 ttcgtataaa cttgtgaagt tttttcaggt cctacttggt caaagaactt ttgcggttct
     1381 tttttgtacc aataatcaaa aatgttctct ccttttccat cacctgaaat ccttccttct
     1441 gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg atattttttc
     1501 atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta tgatattttc
     1561 catcacaatt actttttcat tttcttcctc ttcttttttg attcgtttca acagctgttc
     1621 tgcggcttgt tccccgattt tatctgttgg tggtgtaatc gtgattttcg gaaggatgct
     1681 caaatcgttt ttatccaaat aatcataatg actaaacgca atatccttcc cgattctgat
     1741 accttcttga ttgaaacatt cgatagcacc gatcgacata ttataattgc tgacaaaaat
     1801 cgctgttact cctttttcta ataattcttt tgccccataa tagccacttt ttctagaata
     1861 gtctcctgta aagacctcta attggatcga tggatcattt tgtaccgctt cttttgctcc
     1921 tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc cgattttttt
     1981 atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat cattcactaa
     2041 tattgtatca atacctgctg gtccttctgg gcaattcaat gcgataactg gaatactcat
     2101 ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa tcagcccatc
     2161 gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct gatcggcaaa
     2221 ctcgcttaat aataagctat acccattttc ttcagctact ctttcgatcc ctgagacaac
     2281 ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt
     2341 attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc
     2401 tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg aaactgttcc
     2461 cactgaaaca ccagccatct tagccacgtc acggatcgat gccatacatt ctcactcctg
     2521 ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgctt tctttgcata
     2581 gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac ttcctctcac
     2641 tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc atttttttaa
     2701 aagattgcaa cactttttta aaacgctttc ctaaaatggc gaattttttg cctttttaaa
     2761 attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc ttccatccgt
     2821 ttgaagtttt actttccaat ggtataattt ctttatccta tcaaatgggg gaagtatttt
     2881 gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga ctggctgtaa
     2941 tttgtttggc ggctctgacc aatcctcgga tgaaacaacc gattcttctg tcttaaaagc
     3001 ggaaaaagca gatatcacta acagtgtata tacgctaaaa gtacgtaaag atatggattt
     3061 atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca cgtatcagac
     3121 acaagaaact gattcttctg aatcatctga agaaaaaaca gtgctgacag acatcaagat
     3181 tactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag aagcaaccat
     3241 cacatttacc aaaatcggta attaccgttt gaaggatgag gatggcaaca tctacagtct
     3301 atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta tagtaaagaa
     3361 gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat tgtacatgta
     3421 ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc atatgcgaca
     3481 actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct gttcaaaatt
     3541 atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc tgatttttat
     3601 gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa acagtggatc
     3661 atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg aatataaata
     3721 accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc cgtcgtttgg
     3781 ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta aaatattttc
     3841 cagcaggctt tccttccaat tcagctagtt tttcttggat ctgatggatc accttttttg
     3901 catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt ttaggacata
     3961 catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa taggtatata
     4021 acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata cgttctagca
     4081 tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt ggagcaacat
     4141 catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact tcgatatccg
     4201 ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag atggatcggt
     4261 ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc ttgttcaaaa
     4321 agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc ggcaatactt
     4381 cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca agcatatccg
     4441 tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac atacaaccct
     4501 tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca aaatcatatt
     4561 cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc atcaagttcg
     4621 taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt gccacaccta
     4681 attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata gcaacaacaa
     4741 attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc catccagcgc
     4801 tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt gtgactgttt
     4861 tgttttcctc caacgctttt tcgatacgac ttactcgatt gatttgctcc tctgaaaaaa
     4921 atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata actgattttt
     4981 aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa agattacttg
     5041 atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata attgttgcaa
     5101 gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcaggcg tcagaccgat
     5161 tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc cagggaaaac
     5221 gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg tttctgtagg
     5281 aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat tcggatagat
     5341 acctgtgact tttccgttat tttccagtac tgttttacta atcactccca taatcccgga
     5401 gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc ctaacgtttg
     5461 cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa cagtaagttt
     5521 cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt ttttttaaaa
     5581 taaaagacta gattttgtag ttttttagtg gta
//
LOCUS       DANKHK010000120         5561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000120 DANKHK010000000
VERSION     DANKHK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(260..763)
                     /locus_tag="PW109_002333"
     CDS             complement(260..763)
                     /locus_tag="PW109_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2512118.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1122..2147
                     /locus_tag="PW109_002334"
     CDS             1122..2147
                     /locus_tag="PW109_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2512119.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2470..3348
                     /locus_tag="PW109_002335"
     CDS             2470..3348
                     /locus_tag="PW109_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2512120.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3824..3940
                     /locus_tag="PW109_002336"
     CDS             3824..3940
                     /locus_tag="PW109_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2512121.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4868..5206
                     /locus_tag="PW109_002337"
     CDS             4868..5206
                     /locus_tag="PW109_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512122.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5300..5530
                     /locus_tag="PW109_002338"
     CDS             5300..5530
                     /locus_tag="PW109_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512123.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1876 a          841 c         1033 g         1811 t
ORIGIN      
        1 aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta
       61 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
      121 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      181 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      241 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      301 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      361 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      421 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      481 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      541 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      601 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      661 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      721 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
      781 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
      841 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
      901 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
      961 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     1021 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     1081 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     1141 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     1201 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     1261 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     1321 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     1381 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     1441 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     1501 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     1561 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     1621 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     1681 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     1741 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
     1801 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
     1861 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
     1921 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
     1981 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
     2041 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
     2101 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
     2161 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
     2221 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
     2281 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
     2341 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
     2401 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
     2461 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
     2521 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
     2581 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
     2641 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
     2701 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
     2761 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
     2821 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
     2881 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
     2941 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
     3001 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
     3061 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
     3121 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
     3181 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
     3241 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
     3301 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
     3361 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
     3421 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     3481 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     3541 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
     3601 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
     3661 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
     3721 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
     3781 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
     3841 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
     3901 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
     3961 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
     4021 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
     4081 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
     4141 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
     4201 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
     4261 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
     4321 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
     4381 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
     4441 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
     4501 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
     4561 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
     4621 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
     4681 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
     4741 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
     4801 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
     4861 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
     4921 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
     4981 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
     5041 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
     5101 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
     5161 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
     5221 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
     5281 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
     5341 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
     5401 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
     5461 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
     5521 gcaacagtaa gggagttctt attatgaaaa attttgacac a
//
LOCUS       DANKHK010000121         5538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000121 DANKHK010000000
VERSION     DANKHK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(156..319)
                     /locus_tag="PW109_002339"
                     /pseudo
     CDS             complement(156..319)
                     /locus_tag="PW109_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(672..1361)
                     /locus_tag="PW109_002340"
     CDS             complement(672..1361)
                     /locus_tag="PW109_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2512124.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1406..2170)
                     /locus_tag="PW109_002341"
     CDS             complement(1406..2170)
                     /locus_tag="PW109_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2512125.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2198..2896)
                     /locus_tag="PW109_002342"
     CDS             complement(2198..2896)
                     /locus_tag="PW109_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2512126.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2909..3688)
                     /locus_tag="PW109_002343"
     CDS             complement(2909..3688)
                     /locus_tag="PW109_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2512127.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3704..4648)
                     /locus_tag="PW109_002344"
     CDS             complement(3704..4648)
                     /locus_tag="PW109_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2512128.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4689..5468)
                     /locus_tag="PW109_002345"
     CDS             complement(4689..5468)
                     /locus_tag="PW109_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512129.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         1444 a         1158 c          963 g         1973 t
ORIGIN      
        1 ataatcttca tttctgcaca gtggataata aattcttata gataaaaaat agcctcttct
       61 aaagtcacaa ataccttact cccacatttt aagtaaacac tctgcagcat tctctttgtt
      121 ataaaatgat agatattcct caattttatc tcttattatg ttattatcag ttgataccat
      181 tccattaatg tttcagaata attacaatat tttgggaccg gataactccc ttttgctcgt
      241 aaccactcat atccgttagg attcactaaa agtttaccgt ttatttttcg attctttttt
      301 tgaaccgtcc catgaacata ccgattcaac aagctaaatt ataaaaattg gtgtgctgtc
      361 attatttttt ggaaaattta ttaatggcta tcaatatact tttgtatcat actaaatagc
      421 tcgtcagttc ctatatcgga tataccaata ttaaagcatt tagatcatta tatttaagag
      481 tggtatcatc ccaaaatttc gaatattttc ttttatataa gaataatgat actgaattgc
      541 cccattcacc tttctttttt tagattttct acaagtgatt caaaccctcc ataattacag
      601 ttattacttt tgaaacgata atatgaacat gtgtaatctt ttttattaac aatccatcat
      661 tctatcccta actaatatgc tccattgggc ttgacaataa cataaaacgt ttttaaaatc
      721 aacctcaagt cgtacattaa acttcgatga ctaatatact ctaaatccaa ctcaaccatt
      781 ttatggaaac taacattatt tctatcacta acttgccaca aacctgaaca accaggtttt
      841 acatacaagc gttgaagatc atatctcgtg tactcagcta cttccctctc caaaggtggc
      901 cgtggaccaa ttagactcat atcgcctttt aaaacattcc atagctgtgg caattcatca
      961 atgcttttgg cacggataat tttacctatt ttagtgacac ggggatcttc tttcatcttg
     1021 aacattgcac cttctatttc attttgatca agtaattctt ccaatttttc ttcagcatct
     1081 aaacacatcg aacggaattt atacattttg aatgtttttc catttttacc aacacgtgta
     1141 tggcaaaaaa atatcggact tcctggctct tcttttctga ttcgataagc tacatagaac
     1201 atcaaaggag aagctaaaat caaaccaata atgcttccta caatatcaat cattctttta
     1261 aaaaataaat agatatatcg tttttcaact ttttctttat ccaaccaaac ttgattagga
     1321 tgtacttcac taatacgaga taataaatct tcactttcca tactttccct ctctcctcat
     1381 ttccttttta tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt
     1441 catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     1501 gtactacttt ttccttacca aagtccttag caatctgctc atagcactct tttaagtaaa
     1561 aggtacgctt agagacccca tgggcatcag aagcagccat ctgcacaaga ttatgtttga
     1621 ccatcatttt ggctgttttc tggatgtctt ttccaaatac accgacataa cttggtgcgg
     1681 tgagttgggc caagcatccc atatctagga atggcagcat atgattcggg tctttacgaa
     1741 aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt tcctagttgt aacaactcaa
     1801 aaaagagctg ttctgcatat aacggcactt ccagtgtcgg aaattcgatt agaagatatg
     1861 tatcatctaa atctgtaaat aaaatttcat ctcggtcgat gtcttcgatc agtgttccgg
     1921 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ttgtcttttc tctaattctg
     1981 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     2041 tatgatgggg tgtgcataaa atatgtgtga tcccttgttg aatcgctttt tcagccatgg
     2101 cgatactggc atctatgttt tctgctccgt catcgattcc tggcaggata tggcaatgta
     2161 gatcgatcat ttctcttgtc tcctctcttt ttatttctta attcccgtag taatagccag
     2221 catctttgct gtgttctgcc ccattgtata caacaccaag gactcgcgct tgtaccatat
     2281 ccagcaattc ttttgcttta gtcaatgatt ctttccgtga catattttca cgtacgacca
     2341 ataaggtgcc atccgttttg gaagacatga tctgtgcatc tgttacggca actactggcg
     2401 gcatgtcgaa gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     2461 ttggcgaact tagtagttct gatggatttg gtggtttagg tccacttggc agaatcgaca
     2521 aattctcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagaacgg
     2581 tacttaatcc gcttgcgttg ttcaacttga acgttttata gatgacgggc ttacgtaagt
     2641 ctgcatcgac tagcagcacc cgttgaccag atttggcaaa aacaacggca atgtttgctg
     2701 cagtggtcga tttcccttct ccaggtcctg atgaagtcac aacaatcgtt ttaatttgct
     2761 gaccagctgc ggaggcaaac tggatattcg tccgaatcgt acgatattgt tcagcgattg
     2821 gtgaagaggg gtttgtgagt gtgaccaaac tgactgcatg agtttgggct gctttttgtt
     2881 tctgtgttcg tgccattttt ctttcctcct agactcttga gcggcttctg cgtgatggcg
     2941 ctgttttatc gtttgtagct tttggttgtg tcggagctgg tcctttttta cggataccag
     3001 cattcaattc tttcgctgac atttctggta cagtccctaa gatcgggaag cccaatgtat
     3061 ccgtcacata tttttcatct tttactgtcc ggtcgagtaa ttcaaataag aaagctagac
     3121 ctacgcctac catcattcct aacactaagc caatcactag attcaattta ttatttggcg
     3181 aaacaggtgt cgtattggca actgcacctg agatgatcga gattttatcg acattcatca
     3241 cgtctttggc gttctcttgg aatacttgtg acgttgtatt agcgatttgt tgagccgtca
     3301 ctgcatttgt gcttgttgct tggatcgaga acatttgtga attttgtgac tgattgacgg
     3361 agatggcatc tttgatttgt ccagcagtca tttttacatt gtcttctgtt tctaaacgat
     3421 ctttgacttc atttagtact agatcactga cgatcatatc cttatacgta ttgatcattt
     3481 gcaggttcgt attgacatca ttgacatttg cagtatctga ttgcggcagt gtcacgatca
     3541 attgggcttg tgagctgtat tttggcgtca agatgaaaaa cgtcacgatg cctgagatgg
     3601 ccaagccagc aaacattgag atgaggattg ttatcacgtg tttgcgtaat gtttcaaaaa
     3661 tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat
     3721 tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttggt
     3781 atgagatatt gtcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa
     3841 atgcatcccg ataatctaaa gcaatctttt tcatttcatc aaagctcatg tctgttttca
     3901 tattcgactc tagagcattc aaaacggatt gatagttgct tacaccgttc agactcaaca
     3961 cttttttcgc tacaccttct acgatctttc tttgacgttc ttgtctgccg tagtcaccat
     4021 ttggatcttc ataacgcata cgagaataag acaatgcctg atccccgttc aatgtgattt
     4081 taccgatcgt aaaatcatag ccatcttgtg agaaggtgag gtcattattg acttcgatac
     4141 caccaactgc atcaacgagt tcttttagac ctttcatatt gattgaaaca tagtgatcga
     4201 ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt
     4261 aagcgtggtt gatcttgtct tttgtgtcat gtccaacgat gtcgacatac gtatcacgag
     4321 gaatgctgac aatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat
     4381 ctgatcttcc ttgatcgacc cgacctaaat ctcccgtatc aatccccatc aatagaacag
     4441 agaaagattc ttgtttgtcc agatcaactg gttttgttcg ttctccttgg gttcgttcca
     4501 ctgattcgta agttttttca atagaatgtg agagatctag atataatttt gctcctactc
     4561 ctacgattgc taatactaat acgacaagaa ttgctaaaat aattagaaat atctttttcc
     4621 ctcttgatct tcttcttcgt tttgccatag tttcctccgt ttgctagcct tgcttgaatt
     4681 ctcgcttatt aatagctcgt attgctatag cccatttgct gagcgactgt ttctgctttt
     4741 gacgcgtatg cggctcttat tttttcattc cggatctgtt cgatgagact gagtgcggtc
     4801 aaatattgat cgtaagtagc agtagctgtt acttgaccgt ctttcagcat cgtatcgaat
     4861 agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag cgaatcctag atattgcata
     4921 acgaccgttt tccaagcact atccttaaaa aagctcatat tttcacgtac atgggcgaca
     4981 tctgcacttg ccaacttttc tttgatgatg acatcatcca cagcttgctg ccagtttgat
     5041 acgtttttag taaataattt attgacagcc gtttggatct tcagttttga ttctgcatcc
     5101 tctaactgtg ttaagactgc tttcttttct tcttggatcg cagcagcttc tttaggtaat
     5161 tctgattcct tgatgtcata atcatctgct gaaagtttga tagaagatag tttatgtgac
     5221 aaatttgaaa ctgtttcttc tgttatttca ggatttagga aaattttttc ttttgtactg
     5281 tacagcttat ctacttcttt tgataaagag gtcacggtgt ctttttgatt ggtgatggca
     5341 gattctgcgt aattgatccg ttgatgatgt tgccgctgat tattgagatg gtatccaaac
     5401 aagcaaatca caatagcgcc tgtgcctacc gccacagtag ccaataactt gacgaacggc
     5461 ttttccatag attcactcct aaatattgat gatagataaa taaagatgtg attcattcac
     5521 atcagaatga gcgctttt
//
LOCUS       DANKHK010000122         5534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000122 DANKHK010000000
VERSION     DANKHK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            215..2080
                     /locus_tag="PW109_002346"
     CDS             215..2080
                     /locus_tag="PW109_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2512130.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVASLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFISTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     QAGYLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            2094..3536
                     /locus_tag="PW109_002347"
     CDS             2094..3536
                     /locus_tag="PW109_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2512131.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            3644..4345
                     /locus_tag="PW109_002348"
     CDS             3644..4345
                     /locus_tag="PW109_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512132.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSNDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            4504..4983
                     /locus_tag="PW109_002349"
                     /pseudo
     CDS             4504..4983
                     /locus_tag="PW109_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         1746 a          945 c         1257 g         1586 t
ORIGIN      
        1 ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat tacaagctcg
       61 tttggtgtaa gaagggtata agtcatttat gttcactctc cttgtatgct ttagcgggta
      121 ttacaattgg agtgtaacat aaatggcttt tttatttgtc tcgcttaatt atacaaacgg
      181 cgtttttaaa taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg
      241 tgtatgggaa gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg
      301 gttgcgattc agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc
      361 ggacgtgatc caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt
      421 agcagatgtt tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa
      481 agaagagtcc aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc
      541 ttttctaggg gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt
      601 gatgggctgg ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg
      661 tgtctttacc ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa
      721 ccagttttta gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc
      781 tggtgcggga gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta
      841 tacttcttct gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt
      901 cgtaaaaaaa gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt
      961 agtgatggtt ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc
     1021 tttaggcggt ctcttcaact cgatttatgg atttagtcca atcgtagcaa gtctaatcat
     1081 gggaagttta tggcaagtat tcgtgatgtt cggcatgcat tggggattcg taccgatcat
     1141 gtttttgaat atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat
     1201 cttggcacaa ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg
     1261 agcattaggg atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta
     1321 tggtgtgaca ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg
     1381 tggagctatt atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt
     1441 gacgattccg actttcatta gtacggtgga tggtgtagag tcaaatgtga cagtagcagt
     1501 aatagctaca ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga
     1561 cgaacaaaca caagacaatc aattagaaaa caaacatgcg aatgcaggag aaccgattac
     1621 ttcagcacgg cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt
     1681 accagaccaa gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt
     1741 aggagaacta gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc
     1801 tgtagggatc acaacgactg acggagcgga gattttgatt catatcggga tggatacagt
     1861 agaattaaat gggaatggct ttgagatctt agtcaaacaa ggggatctcg tccaagcagg
     1921 atatttattg attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac
     1981 tccagttgtc attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga
     2041 gattattgca aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat
     2101 cacgtttttc agaaaaattt ttatggggtg gagcaactgc tgccaatcaa ttagaagggg
     2161 cttatgatcg agacggaaaa gggctatccg ttgcagatgc tatgcctgga ggtaagcagc
     2221 gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt
     2281 acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg
     2341 cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg
     2401 gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt
     2461 gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt
     2521 tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg
     2581 ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa
     2641 tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag
     2701 gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagctg
     2761 taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa
     2821 caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag
     2881 aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcac
     2941 gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc
     3001 agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa
     3061 tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa
     3121 atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg
     3181 cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg
     3241 gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt
     3301 tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct
     3361 atacgccttg gggatgtatg gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt
     3421 acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga
     3481 aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa
     3541 agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt
     3601 tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt
     3661 actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt
     3721 cggttcgatg ttaccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac
     3781 tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg
     3841 tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac
     3901 gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt
     3961 ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa
     4021 gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga
     4081 aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa
     4141 aaaaggatat actattgact cattttctaa tgatttctcg gcagtatttt taacagaaga
     4201 agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc
     4261 tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc
     4321 ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat
     4381 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta
     4441 ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca
     4501 cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc
     4561 ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatattcaat
     4621 cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag
     4681 ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt
     4741 ggggcagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg
     4801 ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct
     4861 ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca
     4921 gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt
     4981 taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg aggcaggaat
     5041 ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc
     5101 gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct
     5161 ttcgttgtcg aaaaggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc
     5221 ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca
     5281 tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac
     5341 aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa
     5401 tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca
     5461 aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa
     5521 cataacacga gaac
//
LOCUS       DANKHK010000123         5364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000123 DANKHK010000000
VERSION     DANKHK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(35..808)
                     /locus_tag="PW109_002350"
     CDS             complement(35..808)
                     /locus_tag="PW109_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512133.1"
                     /translation="MKKFAIILTGALLIGTISGCTTSDTSSTSTEISTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDIPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKSYDHPLNLTLNKGILSFVLAGYVDFEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            913..1158
                     /locus_tag="PW109_002351"
     CDS             913..1158
                     /locus_tag="PW109_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512134.1"
                     /translation="MLFLKEEKIIDRENVIGSNIRRIRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1318..2994
                     /locus_tag="PW109_002352"
     CDS             1318..2994
                     /locus_tag="PW109_002352"
                     /inference="COORDINATES: protein motif:HMM:NF024573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2512135.1"
                     /translation="MKKYTKVEKIEINQFRGLKDVTIEFGDRITLICGKNGTSKSTIL
                     GIIAQIFSFSTDYTENPIKRNNLKVYKTLLDKSYESVFSEHFRFSENYDLPGSMDVGI
                     KLYDGIEKKEKNNLKLKLYDSKDRSKSRPVLRGNNDRNVTNPIIYLSVNRLTPIVSRD
                     YDLATDEYIEENKDLALELTNKILLQSSTAIDTTSGTINSLAPHTSEYDFQSISVGED
                     NVGQIVKALLSFKKLKEEYKNYAGGILLIDEVDAGLFPAAQIEFFNVLTKFCKDYGIQ
                     VIMTSHSPTMIEEVFSQRDIKNYKINYLTNTFGKIQVMKDYSWRDIDADIHVRTKKVD
                     EDLMLQKINIYCEDIEAREFLKVLIRKNKLRRLIDVSQTSLGGEQMLRLSDEKVPEFC
                     TKSVIVLDADKHVKDRKNFCILPGEFPPDQLLYDYLRNLPENSNFWNNKYGFTKPVFR
                     RVCNRHLDNIELLHDSEENLQTQIDNFRGSDSNKAGIVRDKFKKFFKDDEIQKLFKLS
                     INKNPFTYWKNDHIDIVNNFERQFIKSLKYCLIIGHKVPKDLVDDYFKDL"
     gene            3007..3894
                     /locus_tag="PW109_002353"
     CDS             3007..3894
                     /locus_tag="PW109_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDL2512136.1"
                     /translation="MPTTYSPLRYPGGKSQLYKFVKKMIDENIGEDGIYIEPFAGGAG
                     VAIQLLIQKDVKMIAINDLDKSIHSLWYSILNYPNEFINLIKETTVNIEEWQRQKEIY
                     KEECDNPFSLKGGFATFFLNRTNVSGIISGGPIGGAKQTGKYKLDCRFNKQSLIKKIT
                     DISSLKDSIILTNMDAEFFIDEISFNYPKEKTFIFFDPPYFTQGKNLYLKFIDKDKHQ
                     NLKECIDKLESYKWIITYDNADEIYEIYKEFHQKYKYQLRYSANNKRKEKAWEYLFAS
                     STTKIDNFANVELLKLVDK"
BASE COUNT         1878 a          760 c          920 g         1806 t
ORIGIN      
        1 ttgaaagaat aattattcct tcaacgtttc ttttttagaa tgttttaatg accaaagcag
       61 tttcattgtc aggcttaggt aattcatgaa atcccgtatt tatagtgctc aattgatcaa
      121 ctaaccatgt tttacttttt gaattgtaga taaatctaaa attaatatcg ttattatcgt
      181 gttgaagggg cgtctccaaa taatctgctg tatattcaaa gtcaacgtaa cctgctaata
      241 caaaagagag tatcccttta tttaatgtta gatttaatgg atgatcatac gactttattt
      301 ttttcaacgt catattgtac atatcttcag ccataaaagt tgagttcgtg atttcatctt
      361 gggtctcatt aaaaaagtta tccgaataat cgcttatttt tgaaaagtcc ttattggcac
      421 cagcctcaaa aaattgttca actttttcag tataaagttt tgctaacgct cctttttcaa
      481 tattaacttt aatattttca tgtctatcaa tttgtgtgct gttcagtaag ggaagatcag
      541 cttttagttt tatatcgtct tgatttcttt caatttcaat tggcaaaatc ttttcagtag
      601 attcagtttc tgataaagga atatctagtt ttttatcatg ataataagct tcaaatccat
      661 ctggaaccgg aaaaaaatgt attgggtaat tcttttctga ggttgttgta ctaatttccg
      721 tacttgtaga acttgtgtca gaagtggtac acccgcttat agttccgatg agtaaagcac
      781 cggttagaat gattgcaaat ttcttcaaaa aaattcctcc ttaaaattga atgtggacta
      841 atactagtcc cattgtagca taccaaatct ttaatattaa taagaaaaag gactaatttt
      901 agtccaaaag gtgtgctatt cttgaaagag gaaaaaatta ttgatagaga aaatgtaatc
      961 ggtagcaata ttagacgaat ccgattagaa aaaggaattg gtcaaaccga gctgattaga
     1021 gatctacagc ttcgtaaaat tgctataact agggaaacgt tggttaagat tgaaggtggc
     1081 cgccaacaca ttaaattgga tcaattgaaa gccatcaaag atatcctaca ggtgtcttat
     1141 gaagacttgc ttcaatgact acttgtagtc atttcgtttg aaattattat gttacaccct
     1201 tatgtgtcac gaagaaatac tggtataata atgaatgagg tatgcaacgt tagggtgtac
     1261 tctaccgttg cagtgttcgt tgttaaaata tgaaaaagaa cagtaggtgt ttttgaattg
     1321 aaaaagtata ctaaagttga aaagattgaa ataaaccaat tccgaggctt aaaagatgtc
     1381 acaattgaat ttggagatcg tattacgcta atttgtggga aaaatggtac ttcaaaatct
     1441 actattttag ggattattgc gcaaatattt agcttctcaa cagattatac tgaaaatcca
     1501 attaaacgga ataatttaaa agtatacaaa acgttgttgg ataaatcata tgaatctgta
     1561 ttttcagaac actttagatt ttcagaaaac tatgatttac ctggttcaat ggatgttggt
     1621 attaaactat atgatggaat agaaaagaaa gaaaagaata atctgaagtt aaaattgtat
     1681 gattcgaaag atcgtagcaa aagtaggccg gtgttaagag gtaataatga tagaaatgta
     1741 acaaatccaa taatttacct aagtgtaaat cgcctaaccc caattgtttc acgtgattat
     1801 gatttggcga ctgacgaata tattgaagaa aataaagatt tggcgttaga gcttactaat
     1861 aaaattttac ttcaatcaag tacagcaatt gatactacta gtgggacaat aaactcttta
     1921 gcaccacata cttctgaata tgactttcaa tcaatatctg taggtgaaga taatgttgga
     1981 caaattgtaa aagccttgtt atcgtttaag aaattgaagg aggaatacaa aaattatgcg
     2041 gggggaatct tgctaattga tgaagtagat gcaggtcttt ttccagctgc acaaattgag
     2101 ttttttaacg tcttaacaaa attctgtaaa gactatggaa ttcaggtgat aatgacctca
     2161 cattcgccaa cgatgattga agaagttttt agccaaagag atattaaaaa ctataaaata
     2221 aactatttaa cgaatacttt tggcaaaatt caggtgatga aagattatag ctggagagat
     2281 atagatgcag atattcatgt gagaacaaaa aaagttgatg aagatttaat gttacaaaag
     2341 ataaatattt actgtgaaga tattgaagca agagaattct taaaggtatt aattagaaag
     2401 aataaattga ggcgacttat agatgtctcc caaacatctt taggtgggga acaaatgcta
     2461 agattatcag atgaaaaagt tccagaattt tgtacaaaaa gcgttattgt tttggatgcc
     2521 gataaacacg taaaggatag aaaaaatttt tgtattttac cgggagaatt tcctccggat
     2581 caattactat atgattattt aaggaatttg cctgaaaact caaatttttg gaataacaaa
     2641 tatggattta caaagcctgt atttaggcga gtttgtaaca gacatttgga taatattgaa
     2701 cttttacatg actctgaaga aaatcttcaa actcaaatag ataacttccg aggttctgac
     2761 tcaaataagg caggtatagt gagagataaa tttaaaaaat tttttaaaga tgatgaaatc
     2821 caaaaattgt ttaaacttag tataaataaa aacccattta cttactggaa aaatgatcac
     2881 atcgatattg ttaataattt tgagcgacag ttcataaagt ctcttaaata ttgtttgatt
     2941 attggtcata aggtccctaa agatttggta gatgattatt ttaaagattt ataggaggtg
     3001 tattttatgc caacaactta ctcccctttg aggtatccgg gagggaaaag ccaattgtat
     3061 aaatttgtaa aaaaaatgat agatgagaat attggagaag atggaattta tatcgagcct
     3121 tttgctgggg gagctggagt ggccattcag ctattaattc aaaaagatgt aaaaatgatt
     3181 gcaattaatg atttagataa atctatacat tctttatggt attcaatatt gaattatccc
     3241 aatgaattta tcaatctaat taaagaaact actgtgaaca ttgaggaatg gcaaaggcag
     3301 aaagaaattt acaaagaaga gtgtgataat cctttttctt tgaagggagg gtttgcgacc
     3361 tttttcctaa atagaaccaa tgtaagtggt attattagtg gtggaccgat aggtggggca
     3421 aaacaaacag ggaagtacaa gttagattgt cgctttaata agcaatcttt aataaaaaaa
     3481 ataactgata tttcttcatt aaaagattca attatactta ctaatatgga tgctgaattt
     3541 tttatagatg agatttcatt taattatcca aaagagaaaa cgttcatttt ttttgatcct
     3601 ccatatttta ctcaaggtaa aaatctttat ttgaaattta ttgataaaga caagcatcag
     3661 aacttaaaag agtgtattga taaacttgaa agttataaat ggattatcac ttatgacaat
     3721 gcagatgaaa tttatgagat ttacaaagaa tttcaccaaa agtataagta tcagcttaga
     3781 tactctgcta ataataagcg taaagaaaaa gcttgggaat atctttttgc tagttcaact
     3841 acaaaaattg acaattttgc taatgttgaa cttttgaaac tagttgataa ataatttctt
     3901 accaaaagca cgccacgcaa cttgaatacg attagaaaac caatcaagat gaattatcat
     3961 cgataaagtg attagctcta ttcaaaaatc gttgaaatac caactatttt caattatctt
     4021 taatgaactc aacttcatgg tcgaagcaga ataacttgaa tcctcaaata acttctctac
     4081 ctgctgtttt ggctacgata ttggctacgt tcccgatgat agtatttgct gatatatacc
     4141 gtcagtctat agtaatttgt taaatcaaat atctctgcaa agcctattcc tttcctctga
     4201 ttcccagaag agtcattgga agggtctgtt ggataacaac taattaattg atcgaaacgc
     4261 tttaatataa acgtttcgat cattttttta tttatcagta tattaactta aaacaaacga
     4321 ccgtccagaa ataatgaact gaacagctaa tattatccat ttgtctgatg aatctaaaga
     4381 cttgaatgtt tactactaga ttgttcaaat tctagtagta gctcaagcaa cattttttag
     4441 tcactacttt gagtgcgaac gacaataata aaatgtatga aagggaatgg ggataatttt
     4501 cttccctttt tactaaatac attcttttcc gttattagag aggactgagc actcaatata
     4561 atcctattcg ttgattatga tgccaaacaa acttgcgaag gctgagcttg ctagtgagta
     4621 acctttttag aaatggagcg agataaattc acttcttcca tttgttcgaa ctaattgggc
     4681 cgtcggtaat gagtatcacg attagaataa acgattggat tcactttgac ttggcgggta
     4741 gcgagtctag cacttaacat attatttact gatcctgcat taagcttgaa tttaatggac
     4801 taactaatga tagcaatatc gtgataatca acaagtagta ataaatgaat tttctctgcg
     4861 ggaattctaa actcaaaatt atctgtcagc caatttcagt tggctttttt cttcttattt
     4921 ggagataaag aatttttcaa catatcctct ttataatttt ttggttaatt tttccgtatt
     4981 tttccaaagt tttctagcct tggttgctgc tatttcttca caccattgcg tcaggtatca
     5041 ctatttggat ttggaactgt actcccagaa ttaatgggta cctttaattt gattttttat
     5101 caaacgccgg aagatgctgt ctttaaagcc cttggggtag aatagacaac tttttattaa
     5161 gctaatatct tcagaaaatt aatcaatgaa cttaacttca tagtttaaac agaaaaaatt
     5221 aattgcttaa ataacttttc catttgctgg tttgaccacg taccaatcta tattacttac
     5281 tagattagtc gttaatctat tcggatcgaa tgggcaaaat atttttgtgt atgttcgtcc
     5341 ttgattatga ttccaaaaag aatc
//
LOCUS       DANKHK010000124         5355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000124 DANKHK010000000
VERSION     DANKHK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            125..1057
                     /locus_tag="PW109_002354"
                     /pseudo
     CDS             125..1057
                     /locus_tag="PW109_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1278..3041
                     /locus_tag="PW109_002355"
     CDS             1278..3041
                     /locus_tag="PW109_002355"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2512137.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAIGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3125..3583
                     /locus_tag="PW109_002356"
     CDS             3125..3583
                     /locus_tag="PW109_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2512138.1"
                     /translation="MYQQVEEYVKKFQKKYPSYQSEKLGFFSFEDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLVYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3692..4375
                     /locus_tag="PW109_002357"
     CDS             3692..4375
                     /locus_tag="PW109_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2512139.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4522..5136
                     /locus_tag="PW109_002358"
     CDS             4522..5136
                     /locus_tag="PW109_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2512140.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNPPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNEIVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5152..>5355
                     /locus_tag="PW109_002359"
     CDS             5152..>5355
                     /locus_tag="PW109_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512141.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1906 a          843 c         1101 g         1505 t
ORIGIN      
        1 gaaaaataaa gaaatcatta tcccagaatt tgactcgcgg aacatcttgc tagctttagt
       61 acgctatgtc aattgggaag gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa
      121 cgagttgatt gattatgtag agactcattt ttttaaaaga agatattcgg aagatgaaaa
      181 gtcctttatc atacgaggaa tagagatcgc aatcggaaga aaggcatttc cccttggact
      241 gaaagaagaa gataagaaaa gcgcagaaac gaagccgtta tttcaattag tttgtcaagg
      301 attggaagag caaaaaggaa ttctagactt acaagaagaa gatgtctatt atattttttc
      361 cttgttcaac agtagaaatt ataccaatga gaacatggag ctgttaagaa aagatttcga
      421 agttgtttat aaaaattttg ttctcaatga cctatcactg aatgaattga ttttacagat
      481 tattagtatc gctgaaatcg acgatgcaga aaattttatt ttcaagcaat cctttttaca
      541 atttgtccgt acgttgtgag ctgatggata agtattttta ccggaaagga tctatcttct
      601 atcagaaaaa gaacaacttc tgtacggaaa aattgtagaa atattggaag agtggaaaaa
      661 gaaaaatcaa atccaactaa gatggaatga aaatttcatt cgaaaattcg tcaaaagtct
      721 ttctttgatc aacagtgaac aagcggggag acaggaaata gaaatttttg ttgtttcaga
      781 aagttcagtc aaacaatttt tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat
      841 cgccgaaata aatcctattt tatttcgttc attagaagaa ttgcctgatg aatgtctatg
      901 tgcgaaaaaa agagtggttt tgtgcgatgc tgctagctat caaaatggat tagaaactga
      961 aaagacaaag atctatccaa tctcattaaa aggtgcaaac atccatttgt atcatattgc
     1021 tcaggaactt cacctattaa aaaaacatat ttcttgatga gaccttaatt aggagacctt
     1081 ctacaaagct ttttaattgt ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga
     1141 tctacttata taacaagctt tctctgaaat ttttttaaaa tgctattgac ttagagtcaa
     1201 ctctaagtag tagaatagat aaagtgtaac aaatctattt tcattaaaag aagatagtac
     1261 ggaggagaga atgtattatg agtacagtat tacatgtatt ggatgcaatc aatagccccg
     1321 cagatgtcaa aaaattatct gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa
     1381 aaatcttgca aaaggacagc gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag
     1441 aattaacgat tgcgtttcat tatgtatttg attcaccaaa ggataaaatc gtttgggatg
     1501 tttcccatca gtcttatggt cataagcttt taactggtcg aaaaaatggt tttgaaaaag
     1561 gacattaccg tgaagtgagc ggatattcca accaacatga aagtaaacat gactatttta
     1621 cagtggggca cacatctaca tctatcagct tagcaacagg tttagctaaa gcgagagatc
     1681 tgaagaaaga atcaggaaat gtggtagcat ttatcggtga cggctcccta agtggtggct
     1741 tagcttttga aggattgaac aatggtggtg ttttacaatc caatctaatc gtattagtaa
     1801 acgataatga gatgtctatt gatgagaacc atggcagttt atataaaaac ttagctgagc
     1861 ttcgtcaaac caacggtaaa agcagcaata acctatttaa agcaatagga tttgattatc
     1921 gttacgtgga taatggaaac gatctggaaa cggtgatccg tttatttgaa gaagtaaaag
     1981 atattgatca tccgattgta cttcacgtac atacggaaaa aggtcgcggt tatcagccgg
     2041 caatcgataa taaaatgaaa tatcattggc atgtaccatt tgatctgtcc accggggagg
     2101 caaagcaagt aacttctgga gaaacattta atatgatcat catggattat ttggataaga
     2161 aaatgcaaac aagtacagct cctattgccg cagtcaatgc agcgatacca ggcgcctttg
     2221 gactgaaaac attccaagaa aaacatccag atcattattt tgacgtgggg atcgctgaac
     2281 agcactctat cactttcgct tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc
     2341 aatcaagtac tttcttgcaa cgggcatatg atcaattatc acatgattta gcgatcaacg
     2401 agaatccagt gattatttta gtcggtggcg gaacgatctc agctagtgac cgaacacacc
     2461 aaggaacatt tgacattcct tacttatcaa gtatcccgaa catcaaatat cttgctccag
     2521 caacaaaaga ggatatgatc tctatgctag attgggcgtt cgaacaaaca gcgcagcctg
     2581 tagcaatccg gattcctaca catggtgtag aacatggtac agctatcgca aatgattata
     2641 gtattccagc ctatcaaaca gtaaataatg gaggaaaagt agcaatttta gctttaggcg
     2701 gttttcttga attaggaaaa gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa
     2761 cgttgatcaa tccaggaacg atcacagaaa atgacgtaga aacattagat aaattaaaag
     2821 aaacacatga attagtcatc acattagaag atggtagttt gtcaggagga tttggcgaaa
     2881 atatcagtcg ttattatggt gattcttcaa tgaacgtatt aaattttggt gcagagaaag
     2941 aattcacgga tagcgttcct gtagggcagc tttatgaacg ttatcattta acaccaaaac
     3001 aaatcgttga agatagtctg cgtgtcttga atattcaata attcattatg ctcaccatcc
     3061 agataattgt ttatactagt atctggatgg ttttttcatt ataaaaagca atggggtgga
     3121 tgttatgtat cagcaagtag aagaatatgt caaaaagttc caaaaaaaat atcctagtta
     3181 tcaatcagaa aaattaggtt tcttttcatt tgaggataca gaaaaaatgg cagacgagtt
     3241 gagcgaacta gttgtaaaaa agataaagac tggtacatca agtggttacg atctgtataa
     3301 acaagagaat gagcctttac caaaagttgg tcaattagac gtggtcctga atggaaaaaa
     3361 tgaaccagtt tgtatcatca aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac
     3421 gaaagagcat gcttggaaag aaggagaagg ggatcggaca ttagtttatt ggcggaaagt
     3481 ccatgctgag ttttttaaac atgcttataa agaagcgttg aacatagaat ttacagaaga
     3541 gaagctggtc gtatacgaag aatttgaagt aattttcttt tagtaagaaa ggtctgctga
     3601 aagaaatagc tcattattga ctcctgaata gaaaagggtt acatttttat atgaagctgt
     3661 atttttatat aacattactg gaggagatat tgtggataca ctggaacaaa tcaaaggcgg
     3721 agtcgtcgtt tcttgtcaag ctttggaaaa cgagccgcta cacagttctt ttattatggg
     3781 gagaatggct ttggctgcaa aagaaggcgg agcagttggt attcgagcaa atacgacaga
     3841 agatatcaat gaaatcaagc gaatcgttga tttaccggta atagggatta tcaaaagaga
     3901 ttacgatggg tcagaagtgt ttattactgc gacagaaaaa gaagtggaag aacttttgga
     3961 gacgcaagca gaaatcattg cactggatgc aacttctcgt aaacgtccga cagaaaaaac
     4021 aactgctgaa cttgtccaaa tgattcacga acacggtcgg ttagctatgg cagatatttc
     4081 gacttttgag gaagcgcttc aagcgcaaga agatggtttt gatcttttgt ctacgacttt
     4141 agcaggctat acagaatatt cacgaaagac gcagacacct gattttgatt tattgaatca
     4201 aatcatagaa caagtcgata tcccagtaat tatggaagga catactgact ctcctgaaca
     4261 agtagagaaa gcatatgaat taggtgcttt ttcagtcgta gttggatcta ttatcacccg
     4321 tccgcaattg atcactaaaa gatatgtcga agccgcaagt aaagcaaata actaaaaaac
     4381 acgcacggta cagattcttg acaaaaaaga aaaattgata gatagtttaa agcagattct
     4441 aagaagatga aaaaagcatt acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt
     4501 ttatagaaag aaggcgaaac catgtttgaa cggagtcctt cagaattgac agaacgtgaa
     4561 aattacaagt tgctgattgg ttcagtcatc cctagaccag tagcagtagt aacgactaaa
     4621 tcagcaaaag atatagtgaa tatcgcaccc ttcagttact tcaatatcgt cagttcgaat
     4681 ccacccatct tatccttggc gatccagaga aaacaagatg cagtaaaaga cacagcaaag
     4741 aatattcttg agacaagaga agctgttatc catatccttg atgaggacaa cgtagaagaa
     4801 gtaaataaaa cagcagccaa tctgcctaat gaggaaagcg aactgtacct gactgacttg
     4861 actttaactg attcagtaat catttcagta ccagcattga cagaagcaaa agttcgtttt
     4921 gaaacatcct tgtatgaaca tgtcgagata cggaataaaa ataaagtaac agctgattta
     4981 tttttgctag aagtacagaa atatcattta aatgaaatag tctatgatga taagacagga
     5041 aggatcgatc caagaaaatt gaatgctgta agccgtttgg caggaaatga ttatgcagca
     5101 ataggagaaa ttttcacgat tgcacgtccc gaataaaaag gagcactagt tatgttaaaa
     5161 ataagctata aaccatcgac agattcaaaa gaaatgaaaa aagaatatga aacagtgaat
     5221 gattttctgc aagggcagta cttagaagtt ccgccattgc aagaccattt tgtcgttact
     5281 actgttactt tagatggtaa ggaaatagaa atgccagatc aaacgattag cggattgttt
     5341 aattacttta acaaa
//
LOCUS       DANKHK010000125         5343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000125 DANKHK010000000
VERSION     DANKHK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            79..1365
                     /locus_tag="PW109_002360"
     CDS             79..1365
                     /locus_tag="PW109_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2512142.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADNEEKVTVWAWDET
                     FNTKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFADLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMDNLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQVNASLKYGLELFATILKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            1502..2371
                     /locus_tag="PW109_002361"
     CDS             1502..2371
                     /locus_tag="PW109_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2512143.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQWMLTDATFKKAFFNTFLYLLVQVPIMLFLALFICNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWAKVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLRLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            2368..3254
                     /locus_tag="PW109_002362"
                     /pseudo
     CDS             2368..3254
                     /locus_tag="PW109_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            3422..4105
                     /locus_tag="PW109_002363"
     CDS             3422..4105
                     /locus_tag="PW109_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2512144.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            4102..4875
                     /locus_tag="PW109_002364"
     CDS             4102..4875
                     /locus_tag="PW109_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2512145.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGFPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKVMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            4872..5270
                     /locus_tag="PW109_002365"
     CDS             4872..5270
                     /locus_tag="PW109_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2512146.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENKDREKSES"
BASE COUNT         1601 a          886 c         1214 g         1642 t
ORIGIN      
        1 aagaaaccgt tttcaaattt tggtatgatg aatgtagcaa acgtaaacaa ctaatggatg
       61 gaaaaggagc ggataaaaat gaacatcaga aagttaattg ctggagtcgt ttgtttagcg
      121 gcagcatcag gtttcttaac aggatgcgga agtgctagta gtacagcaga caacgaagaa
      181 aaagtcactg tctgggcgtg ggatgagaca ttcaatacca aagcagtgaa tgaagcaaaa
      241 aaagtttatg agaacgaaga aacagagata gaagttgtta cgatgtctca ggatgatatc
      301 gttcagaaac tgaacaccgc tttagcgagc gggaatacag aaggattgcc caatatcgtc
      361 ttgattgaag attataggat acaaggctat ttgacttctt atccagatgc attcgctgat
      421 ttgtctgcta ttgtaaagga agatcatttt gcaccgtata aatttgcggt aaacaaagta
      481 ggagataaaa tatacggtgt tcctttcgat tcgggcgtaa cagcaaactt ctatcgtacg
      541 gatctgatgg cagaagcagg ttatacagaa gaggatatgg ataatctaaa atgggacgag
      601 tatattcaag tagcgcgaga tgtcaaagaa aagacgggaa agaaaatcgc tgaagtcaat
      661 ccttctgacc ttggtcgagt aaggatgatt atgcaacagg caggagagtg gtatacatct
      721 gaagatggag agacagtcac gatccaagtc aatgcatctt taaagtatgg tcttgaattg
      781 tttgccacta ttttgaaaga agatctagtc gaacaaacct ctgactggaa tgctggagta
      841 actgcgatac aatcaggagc agtagcttct agcccaactg gcgcttggta ttcttcaacg
      901 atccaaggag cagaaaacca atctggaaaa tggaagattg caccaatacc tgcacttcca
      961 gaaaatctgc aaaaagcgca agcttctaac cttggcggcg ctggctggta tgtgatcaaa
     1021 ggagtagctg gagaagattc agcgaaagat ttcctagaag caacgtttgc ttcgaatgaa
     1081 gaactgatgg gcactcttgc gaaagaaatc ggactagtat ctacgatgct tagcgcgaat
     1141 gaacaagagg cttatcaaga agcttctgaa ttttacagtg gtcaaaagat ctttgacgat
     1201 ttttcaacat ggacatctga aataccagaa gtgaattacg gacatgagac ctatgcaatt
     1261 gaagcagtcg tagctgaagc tcttcatcga atcatcaatg gagaagagac agacaaggta
     1321 ctagcagata cacaaaaaca agtagaagca caattggcta attgatcggt ggtaaaaaag
     1381 tgaatttctt cgctttttca tcagacaaac gaaaagacaa aaaagatcac gtctgcctta
     1441 cagatgttgt ctttcttgtc ttttcatgta gataatcaat gatagaattg gagggaaaag
     1501 aatgaagcag aaagatttat tcaaaaacgg gcatgctttt ttaatcttgc cagtcatttt
     1561 gatatcgatc atggttttta ttccgatggt tttggctttg atcacgtctt ttcaaagtgg
     1621 tccccctgtc aatatgacat ttaacggatt aggtaattat cagtggatgc tgacagatgc
     1681 tacttttaaa aaagcttttt tcaatacgtt cctgtattta ctagttcaag taccaatcat
     1741 gctgtttcta gcgcttttta tttgcaacct tttaaatgat aaaaagctga aatataaagg
     1801 gttgtttcga acagctattt ttcttccgtg tatcacttcg atggtcagtt attctctgat
     1861 catgaaaagt ctattttctc aatctggcct tgtaaacaac ttgttgttgg gcattggtat
     1921 gattgataca ccgattggct ggctgactca tccaatttgg gcgaaagttc tgatcattct
     1981 ttcgatcact tggcgttgga ctggatacaa tatgattttt ttcctttcag gcatgcaaaa
     2041 tattgattcg gagatttatg aagcagcatc tattgatggt gcttctaaat ggcagcaatt
     2101 tatcaaagtg acgatcccga atttaaaacc gattattttg tttacttcca ttacttcgac
     2161 gatcgggaca ttgcggcttt ttgatgaagt acaaaatatt actggcgggg gtccagcaaa
     2221 tgcaacgacc accctgtctc aatacatcta taatttgagt tataaattca cgcctaattt
     2281 tggatatgcg gcagcagttt cctttgttat tgtttccttt attgtgatat tgtcgataat
     2341 ccaaacgaaa gtaggaggag aaagtcgatg aaaaatatgg tagaaaaaga aacaatcgaa
     2401 cgccccaacg tcaaaaaaca agcattagaa cttcgttcga caattgggtc agtattaaag
     2461 tatacagcat taagcatcct atcatttatt tccattttcc cttttgtctg gatggttttg
     2521 ggaatgacga atacacccgt ggatatcaca tcgggaaaaa taaagattgg cagtaacttg
     2581 atcatcaatt ttcaaaattt gttttccaat gagctgaatt ttactcgttc cctttggaat
     2641 tcagcgatga tcgctttggt gaccactgtg tttgcattgc tgatttcgtc aatggcagga
     2701 tatggatttg agatttatca aagtaaacga aaagacctaa ttttcaatat tctactgcta
     2761 tcgatgatgg ttccttttgc ggcattgatg atccctttgt atcgaatgtt ttcacaattg
     2821 aacggaacgg ttttgggaat caacagtttc ttggttgtca tcctgccatc tgttagcact
     2881 gcttttttga tcttcttctt tcgacaaaat gttaaatctt ttccaaaaga tctagtagaa
     2941 tccgctcgat tagacggtct aaaagaacta agtatttttt tagagtgtat atgccgacgg
     3001 ctaaaaatac ctatgccgcg gctgcaatca tcacattcat gagtagctgg aacaattatt
     3061 tatggccctt agtagctttg caatctcctg ataaacggac ggttccgctt gttttatctg
     3121 ctatgggtgc ttcctataca ccagactatg gaatgatcat ggctggatta gtcatcgcta
     3181 ctttaccaac tgccatcgta ttttttgttt tgcaaaagca attcgttcaa ggaatgatcg
     3241 gctctgtaaa ataaaaaaaa cacaataagc gcgttccttc aaagtcattt ttccaagtaa
     3301 aaagatactt ttgggaagcg tgttatttta tgttgagaaa atttaaatga taacgtttta
     3361 agtttgtgct cgaaaactct ttcattttaa ggtaaactta aagagtaaag gaggaggata
     3421 catgctaaaa aattttaaag aagtgattgc tgctatattg ccgatgactg tcctgattgt
     3481 cattttaacg tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg
     3541 tgcggcaatc atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat
     3601 ggaagtcgga gatctagtag ggaaatatat gatcaaaaag aaaagtttga ctgttttggt
     3661 cagtttggga tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt
     3721 gggacaacaa gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat
     3781 cgtcagcgtc ggcactggag tatttttagc ctttgcgcta cttcgggtcg tattcaagct
     3841 atcctattat caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac
     3901 cagttccgaa tttatgccga ttgcttttga ttcgggcgga gtaactactg gtccagtaac
     3961 tgttccgttt attttggccc ttgccggagg gatgactagc atgatcaaac aaaagaaaaa
     4021 tgaaaatgac agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat
     4081 gattttgggg gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc
     4141 gttaccatgg aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat
     4201 atcattagtt ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaggg tttcttgata
     4261 acgactattg ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga
     4321 cagcatttag gcagtgctat tgcgggattt ccttacaatt atatactggt tccccttggc
     4381 tttttgatgg ggttcctagt agggtttgct gagccggcga ttcatgtaat ggtcaaacag
     4441 gttgaagaat tgagtgaagg aaggatccgg tcgaaagtca tgctgatggt gatttcgatt
     4501 gggattggcg cagcagtagg gttgtctatg tggcgtttgc tcgtcggatt ctctctttat
     4561 tatttcttga tcccaggata tgggctagca tttattttag gacgaaaagt ggacaagatc
     4621 tttttagcaa tggcttttga caacggtgga gtagcaacgg gtccgatgtg ttcgacattt
     4681 attttatcaa tgagcgtggc gatcgctgca caaattgagg gaagagatcc gatcattgac
     4741 gggtttggtg tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga
     4801 tatatttata aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg
     4861 ggggaaagca aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg
     4921 acggaggaat gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa
     4981 cgatcttaca tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca
     5041 tttcgccaga aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa
     5101 tggatgcgat tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta
     5161 ttgatgtaga caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa
     5221 tgcaagaact tttagatgaa aataaagata gagaaaagtc tgaatcatag acaagcgttt
     5281 gaagaggaaa taaacataaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt
     5341 att
//
LOCUS       DANKHK010000126         5244 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000126 DANKHK010000000
VERSION     DANKHK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            6..362
                     /locus_tag="PW109_002366"
     CDS             6..362
                     /locus_tag="PW109_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HDL2512147.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            378..728
                     /locus_tag="PW109_002367"
     CDS             378..728
                     /locus_tag="PW109_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HDL2512148.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            757..1536
                     /locus_tag="PW109_002368"
     CDS             757..1536
                     /locus_tag="PW109_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HDL2512149.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1547..2224
                     /locus_tag="PW109_002369"
     CDS             1547..2224
                     /locus_tag="PW109_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HDL2512150.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2243..3349
                     /locus_tag="PW109_002370"
     CDS             2243..3349
                     /locus_tag="PW109_002370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDL2512151.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3339..4430
                     /locus_tag="PW109_002371"
     CDS             3339..4430
                     /locus_tag="PW109_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDL2512152.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4443..5183
                     /locus_tag="PW109_002372"
     CDS             4443..5183
                     /locus_tag="PW109_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDL2512153.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1678 a          863 c         1251 g         1452 t
ORIGIN      
        1 caatcatgat taaagtagtt attgcagacc gtatgggtaa aggacaaaat gtggccaaag
       61 gggtagaagc agctggtgga aaagcagttg tcgtaccagg aatgggggca gatatgcgtt
      121 tgggcgatgt gatgcaacaa gagcatgcag atatgggtat ctctttttgt ggtagtggtg
      181 gtgcaggtgc attaacagca gcaacaaaat atggctatcc agaacgacat ggtatgcgtt
      241 cgattgaaga agggatcaca gctatcaagg atggcaaaac tgttctaggt ttcggcttta
      301 tggatcaaga ggaattaggg aaacgtctga cagaagaatt tatcaaaaag catggaaaat
      361 agaaagaggg acatgaaatg agcgttcaaa agacaattca gcaaaaagtt atcgtctccg
      421 gtaaagggga aacaaagcaa catgcttttg catcagcgtt agcagaaatt caaaacaaaa
      481 taatggaaca atcaaacgta ttattgcgaa tcgaaccagt caatgtggat attttaaagg
      541 cggtcgaaaa caaatacaca gaacgctttc tatttttctt cttcccacgg caacgtatta
      601 gctatgaagt cgaactctct gttactgtgg ctgtaacaga aataaaattg gcagacatat
      661 cgttcagcca gcaaatgact gaagatccaa atgggattgc cattccattt gtaactaaaa
      721 aaatctaatt ttgaatcata agggggaaag aataacgtgg atttcgtaat cattctagtc
      781 aaatcggtca ttatcggggg attacttggt tttgcagcat ctgtcggggc agctcgaatg
      841 tttcatgcac ctaatacaca aggtttagga gcttttagaa cattaggcga aatgaatgcc
      901 tgcatgggtg atccagcttc tcatttttca tttggtttag gctttttctt taatgcatgg
      961 gcttcttcag ttggtgctgg tgcattcaca caagatgtga cgcatcgaat cattcccaat
     1021 tgggcagcgg cagcacttct agcaaaaaac aaagatgtca cccagaccat gcatgatccg
     1081 aaaaaaatgg ggatcattgg agcggttatc ggggcagtgg tggtagcctt cttaaacaca
     1141 acagctgcag caattccaga ggcgttacaa gtgacggcgg tttccgtttt ggtaccagca
     1201 gcgacgattc tgatcaatac agtaatgcca gttatttttt ggttggcagc tttagacgca
     1261 gggaaacgaa ctggtttttg gggcactctt tttggtggta tcgcgcaatt gattatggga
     1321 aatgcggtac ctggtgttgt tctaggaatc ttgattggta aaggcgtcga tgagagcggt
     1381 tggaatcgcg tcacgaagag catgatgatc gctattgttt tattatttat tttcagtgga
     1441 tttttccgtg gatttgatat ggaaatgatc aagagcttcc agcttcaagt gcctaattgg
     1501 cttgataact tgcacaacat ctttagtgtg aaataggagg agaatcatgg agacaacaga
     1561 agaacaagca aaagtattag aagcaaagcg gcaaaagagc ttttggtttg cagactggtc
     1621 ttttccgcta atcgtaggaa ttatgtccgc tgctgtattt gcaggaacac acatgtatgt
     1681 gatgtatggt gtgggtgcat tcaatgaagt ttcgatcgtg gcgatgttaa aagccgggct
     1741 ggatggcgga tcctatggcg cagcggcagc gtttggtgct agtttcttat ttgccagaat
     1801 tttagaaggc tctttagttg ggatcttgga cttaggcggt tcgatcctaa ctgggatcgg
     1861 aattggggta ccagcaattc tcttaagtat gaaaatagcg gcaccaatcg ataattttgc
     1921 actctcttta ttgactggtt tggttctagg tttgattgtt ggtggcatca ttgtcttgat
     1981 tcgtaaattt acgatcaatc aatcaaattc cacttttggt gctgacgtca tgatgggggc
     2041 aggaaattca tctggacgtt ttttgggtcc attaattata ttaagcgcgt gtgcagcatc
     2101 tattccaatc ggcattggcg ctacattagg agcactagga ttctatgctt ggaaaaaacc
     2161 aattgctgga ggtgctattt taggagcaat gattttagga gcaatcttcc cggcagtaaa
     2221 ttaatagaaa gagggtcatt aaatgtttga tactttgatt aaaaatggaa ggttagttga
     2281 tggatctaaa atagaggtcg ctatagaaaa aggcatgatt aaagcagttg cagcagaaat
     2341 aaatcagccg gcgcaagaag tagttgactt ggagggaaag tattatttat ccgctgggtg
     2401 gatcgatgat catgtccact gctatgaaaa gatgaatctt tattatgact atcctgatca
     2461 gataggggtc gaaaaaggtg tgacgacggt cattgatgct ggaactacgg gagcagaaaa
     2521 catccgtgaa ttttatcaat tgacgaaatc ggtcaagaca aatgtgtatg cgctaatgaa
     2581 tatctctaaa tggggcattg tcaaacaaga tgaacttgct gatctaacaa agattcaaga
     2641 agatctagta agtactgcgt tagcagaact gccggaattt attgtcggga ttaaagcgag
     2701 aatgagtaag acagttgttg gtgaaaatgg cattaagcca ttagaattag ctaaaaaaat
     2761 ccagacagaa aacaatgatc tgccattgat ggttcatatc ggttctgcac ctcctgagtt
     2821 agaagaagta ttgtcaagaa tggaaagagg cgatgtattg acccattgtt ttaatggaaa
     2881 accaaacggt attttagatc caaagacaga tcaaattaaa aactttgtct gggatgctta
     2941 caataatggt gtcgtctttg atattggaca tgggacggat agctttaatt tccatgtggc
     3001 agaaaccgca ctaaaagcag gaatgaaagc tacctctatt agtacggata tctacatccg
     3061 caatcgtaca aatggcccag tttttgatct agcaactact ttagaaaaat tgagggtagt
     3121 cggctattct tgggaagaaa tcattgaaaa agttacagca gttcctgcac ggaattttca
     3181 tttgaagaat aagggccagt tagctgtcaa ttatgatgca gatttgacga tatttgagat
     3241 tgttgaaaat gaaaaggaat tgaaagattc caacggattt acacgtatag ctaaagaaca
     3301 aattttaccg attaaaacaa ttattggagg cgtcatatat gacaattagc tatggaaagt
     3361 ttgatttaaa agaagtcatt aatgcatcgg gaaaaatgac gatcctaggg gtttctaagg
     3421 tatctgaaag tgtcctagca gcacaacgat ttggaggcga gcattttttt gagatgagtg
     3481 atcttgccat caaaaccggc gcttatcttt ctcaattact gaaagtggaa gatgcgcaaa
     3541 tcgtttcttc tgcttcagca gggattgctc aatctgttgc tgctttaatc ggacagggcg
     3601 acatgtacca tgtctatcat ccctatacag aaaaaatcgc tagacgggaa atcatcatac
     3661 ctaagggaca taatgtagat tatgggacac cggttgaagt aatggttgct caaggtggag
     3721 gaaaagtcat agaagctggc tatgcaaata tgtgtacacc ggaacatgtt tccatgatga
     3781 ttacggaaaa aactgctgcg cttctatata ttaaaagtca tcatgcagtt cagaaaagta
     3841 tgctgacggt ggcagaaatg gtagaagttg ccaaagctca tcgtttgcca ttgatcgttg
     3901 atgcagctgc ggaagaagat ttattcaaat atagcgaaat gggcgttgac ttagtgattt
     3961 actctggggc aaaagcaatc gaaggtccga gtgccggact ggtcatagga aaaaagaagt
     4021 atattcaatg gattcggcta cagtctaaag gtattggccg agcaatgaaa atcggtaaag
     4081 ataatatttt aggctttacc caggcactag aagattattt aaaaaatggg agcgaatccg
     4141 gtgactccat gaagcaacga ttagcgcctt ttgttaacgc aatcaatcaa cttcctgacc
     4201 tttcagcgaa aatagttcaa gatggtgccg gcagagatat ttatcgagca agtgtaacga
     4261 ttaatggcga taaaagtgcg aaagaagtca ttcaggaatt aaaagcacga aatccagcag
     4321 tctacacccg agaatatcaa gcaaataacg gaatcattga gttcgacatt cgttccgtca
     4381 gccaaaacga aatggaaaaa attataaaaa gattgagaga aatcatgtag gaggctttta
     4441 aattgtcagt tatcccaaat tattataaaa atcgtgtatg tttaaatgta ttagctcatt
     4501 cagttgaaaa tgcaaaagca tgctatgaag cagcggaagg gcatgtcgta ctaggtgtgc
     4561 tatcgaaaaa ttatgctagc gatgaagcag caatcgaaga tatgaaaagg taccaagacg
     4621 caaccaataa tgcgttgtca gttggattag gcgctggaga tccaaaccaa agtcagatgg
     4681 tttcgcgttt atctggggtt ttacaaccac aacatgtcaa tcaagtattt actggcgtgg
     4741 ggacttctcg tgctttattg ggccaaaatg atacagtcat caatggattg gtttcaccaa
     4801 caggtaagat cggctatgtc aatattgcta caggtccgtt aagttcacaa gcgccagcag
     4861 ctgaagtgcc tattgaaact gccattcaaa tgctgcaaga catgggaggt acttcaatca
     4921 agtatttccc gatgaaaggc ttagcacata gggaagaatt taaagcagtt gcggaagctt
     4981 gcgcaaaata tgaattctat ttagagccaa caggaggaat tgatctttca aactttgaag
     5041 aaattctgaa aattgcaatc gatgcaggtg taaaaaaagt gattcctcat gtttatagtt
     5101 caattataga taaagaaaca ggagatacgc gtataggaga cgtaaagata ctattaagca
     5161 tggtaaaaaa aattattcac taactcatta gaaaataaga agcttattcc agaatgaaat
     5221 taaatggaat aagcttttta taat
//
LOCUS       DANKHK010000127         5178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000127 DANKHK010000000
VERSION     DANKHK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..614)
                     /locus_tag="PW109_002373"
     CDS             complement(<1..614)
                     /locus_tag="PW109_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2512154.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENK"
     gene            797..1648
                     /locus_tag="PW109_002374"
     CDS             797..1648
                     /locus_tag="PW109_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512155.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            1669..1952
                     /locus_tag="PW109_002375"
                     /pseudo
     CDS             1669..1952
                     /locus_tag="PW109_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2173..2469
                     /locus_tag="PW109_002376"
     CDS             2173..2469
                     /locus_tag="PW109_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512156.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            2471..2893
                     /locus_tag="PW109_002377"
     CDS             2471..2893
                     /locus_tag="PW109_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512157.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVYARVDPFDPTKILKR"
     gene            2912..4504
                     /locus_tag="PW109_002378"
     CDS             2912..4504
                     /locus_tag="PW109_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512158.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRTLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAESLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            4516..4851
                     /locus_tag="PW109_002379"
     CDS             4516..4851
                     /locus_tag="PW109_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512159.1"
                     /translation="MKRYLNQWKVIEGSLKKERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            4991..>5178
                     /locus_tag="PW109_002380"
     CDS             4991..>5178
                     /locus_tag="PW109_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512160.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT         1935 a          776 c         1024 g         1443 t
ORIGIN      
        1 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
       61 gataatcgtt caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
      121 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
      181 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
      241 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
      301 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
      361 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
      421 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
      481 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagcaatg cgcagttttc
      541 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
      601 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
      661 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
      721 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
      781 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
      841 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
      901 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
      961 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     1021 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     1081 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     1141 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     1201 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     1261 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     1321 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     1381 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     1441 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     1501 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     1561 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     1621 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     1681 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     1741 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     1801 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     1861 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     1921 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     1981 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     2041 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     2101 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     2161 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     2221 gatcgaagat gtgacgaact aagtaaccgt acgggtaata aatggagtcg taacgattat
     2281 ttaaaattac taatagaaaa tgattttgat cgtcctttga tggaatataa aaaagaaaag
     2341 tttgatcagt tattagaaaa gttttcagaa attcaaagct ataacacaaa gatattagaa
     2401 gaatatgtta gccaaaacaa tcgtatttta gaaatcttga tcgagcaaaa tagaggaagt
     2461 gaactttgat atggcaaagg ttatagacaa acacatttac tttcaccaaa cacatatcga
     2521 tgtgttggat gagattattt ctactcatgc agaagttaaa aactatagtg aagccgttcg
     2581 gtttctatgt atgaattatg atccagaaat ggagaaaaaa gtaaattcca atcaagcaaa
     2641 gttgaatgga atgagtaaag aaatttctat tttaaccgaa atggtttcag agtttgcaga
     2701 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaacggttt accaagaagc
     2761 aaaagaacgt gtggaaggaa aaattaaatc taatcaaacc aaaaaatatt cccctcagtt
     2821 gaagaagcct gtgaatgaat ctgtttatgc cagagtagat ccatttgatc caacgaagat
     2881 tctaaaaaga taaaagtaga aggggaacat gatgaataaa tcgcctgcag tcattctcac
     2941 ttgtcaattt accttgccca ataaaaaatc tttttcaaca tacattgatt atatgacaag
     3001 agaaaaagcg ctggaagaaa tggaacgacg tacgcctgaa gaagaacagg aactgcaacg
     3061 aatcaagaat gctttagagg atttttatat tcctgaagga gaaacttttt cggagagtaa
     3121 gacggataaa gaattcagcg aaacgtcgaa tgaagctcaa tctttgatgg gaaatggaat
     3181 caactttgac aagttagagg aacaagattt taccaaatat ctttcttata tgacccgtca
     3241 ctatgcgtta gaacaaaaga aagaactgac gcaaagtgaa caaaaagaat accaacgctt
     3301 gaaccaagca ataaaaaaat atcaaacaga gccacacaaa aaagcttcag gaaaccttcc
     3361 tggagttttt tctatggctt cagacgaagt gaaaggaagt gatctgaagg agattaagca
     3421 aatcttccaa agaggacaac aaaaaggctc aatcatttat caagatgttg tctctcatga
     3481 taattcgtat cttgaaaaaa taggattgta cgatcccaaa acagatactt tagatgaaga
     3541 aggattaaaa gcagctggaa gaaaaatgat ggagacatta ttcgagaaag agcaactgaa
     3601 tgaaacaggc tactggatgg caacgattca ccgcaatact aaacatatcc atattcattt
     3661 tgcagttgtt gaaaaagaaa atacgcgtaa accttacact gaggttgaaa atggtgtgag
     3721 ttatatcgta ccgaaaggta agagaagaca agcaacatta gacgcaatga agaccaatta
     3781 tgtacatgag ttagaacgtt tttcttatga aaaggatcga atgattggta tggagaaacg
     3841 aaatttattg acacgaaaaa gtgatttgcg taacagctta acaaaagtgg taaaagagcc
     3901 agtacgttat gatcgacacg caatgaactt attaaatgaa gtatatagaa ccttacctga
     3961 aaaacgttcg gaatggaatt atggggatga aaatcgaact aaactttcac catacacaag
     4021 agagaaactc gacgagttaa cccaatatgt gttaacaaaa gaacctgaat atcaagaata
     4081 tatacagatt tcaaaagcat tgaaagaaga agcagaaagt ctttatgggg aaagcaagag
     4141 agaatcaaag gatgcagaga aaaatgctct ctttgattta aaaaagcgca caggaaatgc
     4201 catcctaaca gagctgaaga gacaggatag tcaaataaaa ccattaatag aaagtgttga
     4261 tcagatgcaa tctatttaca atgaacgatc ctttcgaacg cttaaacaat tgccaacgaa
     4321 tgaatacaaa tatagttacg aagaattcat gaagcgcagg aaacaattcc aacaaagagt
     4381 tgtcacacag cggagtttac gtcaattaaa tcgaacgatt aatgcccatg aagaaaaata
     4441 ccacgcaagt aaagcatacg aagagcaaca acgaagaatt caaatggaac aagaaagaca
     4501 gtaggaggaa gagaaatgaa acgatatttg aatcaatgga aagtcataga aggatcacta
     4561 aaaaaagaac gaatcgaaca acttcccgat tgtttggaaa aagagcattt gttccagata
     4621 agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt tgagtaccca
     4681 gctactggtg tgtattgttg taatcatgtg aatggtgaaa aatattttat cattcaagaa
     4741 tatgagggca agttagcacc ttattatact acttgggaaa tgaatgaaga gggaatcaat
     4801 aactttccat gtaaaagcat tgaagagtcc atttcactca cagagtgtta gttgttaagt
     4861 gaataaaaga agtaaaagag tcctgaacgt gattgtccag gactcttttt ttcaatgaat
     4921 tgttttccgc caatttattg agtggtgcga tttccgacta cattatatca aaagaaagga
     4981 ggaagaagaa atggcttatt caactattgg agttgttgtt gaaaaaagca gagacaatct
     5041 cgtttttgtg acagaaattc aaacaggcag atcttttgtc gtcacagaca aagcggcaaa
     5101 agcttatcaa agtggcgata ttttaacgtt aaatctgact acgaagacgt tcgtcgatgc
     5161 gacagaagat tatccatt
//
LOCUS       DANKHK010000128         5095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000128 DANKHK010000000
VERSION     DANKHK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            22..1956
                     /locus_tag="PW109_002381"
     CDS             22..1956
                     /locus_tag="PW109_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2512161.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2413..2829
                     /locus_tag="PW109_002382"
     CDS             2413..2829
                     /locus_tag="PW109_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2512162.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2843..3409
                     /locus_tag="PW109_002383"
     CDS             2843..3409
                     /locus_tag="PW109_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2512163.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3432..4418
                     /locus_tag="PW109_002384"
     CDS             3432..4418
                     /locus_tag="PW109_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2512164.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4570..5095)
                     /gene="scm"
                     /locus_tag="PW109_002385"
                     /pseudo
     CDS             complement(4570..5095)
                     /gene="scm"
                     /locus_tag="PW109_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
BASE COUNT         1530 a          946 c         1131 g         1488 t
ORIGIN      
        1 tctataagga gcggatgatc aatggcaaag caaaatattg tcgttgtagg agcaggatat
       61 gccggtgttt cagctacaaa atacttggca aagaaactga aaaaagaaga tgtagcaatt
      121 actctgatcg atcgtcattc gtaccatact atgatgaccg agttgcatga agtagctggc
      181 ggacgtgtag aaccagaagc aatccaatac gatctgcagc atttgtttgc tcgtcaaaag
      241 aatgtacaac ttgtcacaga cactgtaatc ggtatcgata aagaacgaaa aatcgtcaag
      301 accaaattag gttcctatcc ttttgatcag ctgatccttg gaatgggcgg agagccgaat
      361 gactttggta cacctggtgt caaagcacac ggattcaccc tttggtcatt tgaagattct
      421 gtaaggattc gggaacatat tgagaagaca gtagcaaaag ctgcattgga accagatgat
      481 gaactgcgta aagccatgct gactttcgtt gtctgtggtt ctggatttac cggtattgaa
      541 atgattgggg aactgatcga ctggaaaaat cgcctagcca aagattataa attagatcca
      601 aacgaaatca cactgatggt cgtagaggca atgccgacga tcttgaatat gttggatcga
      661 aatgatgctg caaaagctga acgctatttg cgtaagcaca acgtggaatt attattgaat
      721 gcaccaatcg tagaagtggc tgctgatcat atcaaattga aagacggacg caacattccg
      781 actcacacac tgatttggac agctggtgtc aaaggaactt ctgacgctgc tgactttgga
      841 ttagaatcag cacgcggaca gcggttgatc gccaatgaat atatgcaggc aaaaggctac
      901 gaagataaaa acatctatat cattggtgat ttagtctttt atgaagaatt cccaggaaca
      961 ccgacaccgc agatcgttca agctgctgaa caaacaggac acacagccgc agcgaatatc
     1021 gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag gaaactatca aggatttatg
     1081 gtttctattg gtgccaaatg gggggtagct aacctatttg ataagatcca tttaagcgga
     1141 tttttagcta tcgtaatgaa acatatcgtg aatttgaaat acttctttga tattcgttct
     1201 ggttattata tgttccaata tttaatgcat gagtttttcc atatcaaaga tgatcgcaac
     1261 gtgactcgag gacattcttc tcgttacggc aatgtcttat ggagtgtgcc acttcgtatt
     1321 ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa tcattgggaa tggtgaagtg
     1381 ttcaaaccaa gtacttggtt tggtgaagga tcatggttca ctggaaacag tgtgttccct
     1441 tggacatggg aatacaatgt aacagatgtt gccagtggtg cttctgctgc tggtgatgca
     1501 accacagctg ccagcggagc aggggcaagt gccggagaag cagcgactca agctgcacat
     1561 tttggtttga gttatgcata cggtgaagaa ccaatggctg tgattgagaa gtcaccagac
     1621 tggttcacaa agatcatgca agtgatcatg ccaaacaatg atgtagcaat gttcttccaa
     1681 aaattcatgg tattctttga aattgccttg gcattagcat tgattgctgg attattcaca
     1741 tggctatgca gtgcgacaac gattgtattg gtgatcacgt tctgcttatc aggcatgttt
     1801 tactgggtaa acatttggtt catttttgtt gctttcgcat tgatgaacgg ttccggacgt
     1861 gcgattgggt tggatcgctg ggtaattcca tggctacaac gtacactagg taattggtgg
     1921 tacggtagac caagttccca gtacggcaag aaataaatag ataaagaacg ttatttgaat
     1981 gcaagatttc aatagaaagg ggctgagaca aaccgtcaca gcctcctttt tatgtaaaat
     2041 taaaggaata agatgcccga gataagcctc catgagcaag acacaggtgt cacaacctct
     2101 gttacaacct cttgttataa acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
     2161 atttcacaaa aacttgaaaa gtaattttcg taaattcctt cttatttctt gaagttgccc
     2221 acttctgtca caacctcttg ttataaacgg tgttccacct gctgcttctc ggtaataagc
     2281 ccaaagaatt agaaaacatg gaaagctgtt ttctattctt cgttcttatt actcaaagca
     2341 agacacaggt gtcacaacct cttgttttta tgctaaaatt gggtgagtta atctggaaaa
     2401 aggagaacaa aaatgagcct taaagaattt gctgaaaaaa gtctgctcaa accttgggat
     2461 gtttttatta ttttgctttt gactgttctt tcttttttac cagtcatgat ttttggtctt
     2521 cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc gtgtggatgg cacagaaatc
     2581 aaaacattcc ctttgatggc tggagaagag agcaacacat atacttacaa agatgaacac
     2641 ggagattata acttgatcga gatagatggc gatcgagtac ggataaaaga agcagattgc
     2701 gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa atggcgaaac catcgtctgt
     2761 ttgccacata aattagtgat cgaagtccgt tcaacagatg gaggtgacga agacagtctg
     2821 atttattaga ccttcacaca ccatgagtaa attacaaaaa atgatttata tttcgctact
     2881 tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg atcccttatc ctttttcttt
     2941 tgcgcctggt gcaaaactag gcttagccaa tctaatcacg atcgttgcca tttttaccat
     3001 gaaaaaacga gacagcttct tactactttg gctacgacta ttcctgacta ctttgctagg
     3061 cggaacgatt tccacattcc tttatagtat gagtggtgct ttgcttagtt attttggaat
     3121 gttattggtc aagcaattag gaccaaaacg tgtcagtatc atcggaatta gtgcggccgg
     3181 cggtttcatg cataatgtcg gacagctttt gatggcttct ttcattgcac aatcatggac
     3241 agtcatgttg tacttgccag tcctttcagt tttagggatt ttatcaggga ttgcaatcgg
     3301 gatcgcagcg aattatcttt tacgacatgt gcagacactt caacgattcc aaaaagatta
     3361 tgagcaggga accaaacaaa aatggagccc aatgtttacc aaaaaataat cgtcacacaa
     3421 ggagtttttt tatgaagcta catccaatgt gggaaaacta tcctgcttta agtaaagaat
     3481 tgacagaaac actaaaacgg atggaagaaa ctgtccgcct gaaaaacaaa cctgtcgaac
     3541 aagcaatcaa agaaacgatc catgccggcg gaaagctgtt gcgtcctgcc tatcagctat
     3601 tgttctcaca atttggacca gagcaagatc gagacaaagc catcgctttg gcagcttcta
     3661 tcgaaatgct ccatacggcc acactgatcc atgatgatat cgttgatgaa gcggatctgc
     3721 gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt cgtttctgtc tatgcaggag
     3781 actatttatt tgtctgctgt tttaaactat tgtctgatta ctccacttcc ttgaaaagtc
     3841 tccaattgaa ttctcgcagt atggaaaaag tattgaatgg cgaattaggg cagatggatg
     3901 atcgctataa ttatgaactt tctgtaaaag aatatttgga aaatatttca ggtaagacag
     3961 ctgaactgtt ccaattaagt tgttctgttg gcgcttttga aagtggaaca agcgagcgct
     4021 tcgcaaaaaa agcaggagat atcggacttt ctatcgggat ggcctttcag attatcgatg
     4081 atattttaga ttatacaaaa gagagccagg aaatcggcaa acctgtacta gaagacatgc
     4141 gtcaaggggt ctattcttta ccgttgatct actctttgca aaaaaacaaa ccaaagctac
     4201 taccttattt agagaagaaa gcggcaatga cagaagaaga tgtcgacgcc gtacgcaaaa
     4261 tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt agcggcctct tacacagaaa
     4321 aggctttgaa ggaaataaag aaattacctg ccacctctct tcgaacaaaa gagaatctct
     4381 tttcccttac ccaactgatt ttggatcgca aagactgaat agggaaaggt tcgatacgta
     4441 aataaatttc tcagaaggaa atctacgtgt tttttgacaa attgaattgt tcttgtattt
     4501 atgataaaaa gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg
     4561 aatatttttt taccttttcg atttcttata actcataaaa gtgattctta aaccgattac
     4621 aagtaatata acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag
     4681 tgctcttttt gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt
     4741 cctgttatcc gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt
     4801 agacgacgtg tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga
     4861 ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg tagtggtaga
     4921 tgaatctgag ctatcacttg tggtagaggt agtatctgta ctgtcacttg aagctgacgt
     4981 gtccgtgcta tcacttgttg tggtagatgt atccgtactg tcgcttgaag ctgacgtgtc
     5041 cgtgctatcc acttgtggta gaggtagtat ctgtactgtc acttgaagct gatgt
//
LOCUS       DANKHK010000129         5088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000129 DANKHK010000000
VERSION     DANKHK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..292)
                     /locus_tag="PW109_002386"
     CDS             complement(<1..292)
                     /locus_tag="PW109_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2512165.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPV"
     gene            627..1061
                     /locus_tag="PW109_002387"
     CDS             627..1061
                     /locus_tag="PW109_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2512166.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1084..1404
                     /locus_tag="PW109_002388"
     CDS             1084..1404
                     /locus_tag="PW109_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512167.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1545..1736
                     /locus_tag="PW109_002389"
     CDS             1545..1736
                     /locus_tag="PW109_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512168.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1807..2064)
                     /locus_tag="PW109_002390"
     CDS             complement(1807..2064)
                     /locus_tag="PW109_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2512169.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2222..2485
                     /locus_tag="PW109_002391"
     CDS             2222..2485
                     /locus_tag="PW109_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512170.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2509..2697
                     /locus_tag="PW109_002392"
     CDS             2509..2697
                     /locus_tag="PW109_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512171.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2780..2965)
                     /locus_tag="PW109_002393"
     CDS             complement(2780..2965)
                     /locus_tag="PW109_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512172.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3180..3551
                     /locus_tag="PW109_002394"
     CDS             3180..3551
                     /locus_tag="PW109_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2512173.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3701..3773)
                     /locus_tag="PW109_002395"
     tRNA            complement(3701..3773)
                     /locus_tag="PW109_002395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3738..3740),aa:Gln,seq:ctg)
     gene            complement(3819..4247)
                     /locus_tag="PW109_002396"
     CDS             complement(3819..4247)
                     /locus_tag="PW109_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512174.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4307..5065)
                     /locus_tag="PW109_002397"
     CDS             complement(4307..5065)
                     /locus_tag="PW109_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2512175.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1758 a          798 c         1009 g         1523 t
ORIGIN      
        1 ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca gagccttctg
       61 ttgggattgt taattgttgt cctgagtaga tcaggttaat atcagaaatt gaatttgatt
      121 ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc gttgatagag
      181 tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc atgaagaatg
      241 cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc atgaaaaatc
      301 tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt taacacgtga
      361 aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt ttttgtaaca
      421 gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca gaaataatgg
      481 gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa gaaattgctt
      541 ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt gagatgttac
      601 aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc atggtagcag
      661 ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta gcaaaggaca
      721 atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc catagtgcct
      781 atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc atgctgaaaa
      841 aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt gaaactggag
      901 atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat ttgatcgtta
      961 tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc gcttcttatg
     1021 tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag ggaggaatag
     1081 aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat tttagcaatt
     1141 attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa gtggaaagga
     1201 aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg attccctgtc
     1261 acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat ggaattatac
     1321 agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt aacagaagag
     1381 cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga aaactgatta
     1441 gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa tgcgtataac
     1501 ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa gcaggaaaag
     1561 tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg aaaagcctga
     1621 tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa tttatagaat
     1681 taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca gaatagaaaa
     1741 aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt taaacatgcg
     1801 agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa aaattactga
     1861 aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat gcctaaaaga
     1921 aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg aaaacttctt
     1981 ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag gaatataaaa
     2041 aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc atgatacaaa
     2101 ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta aaatctgtat
     2161 cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga gtgggatttt
     2221 catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc gggtgattta
     2281 ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa atggtatttt
     2341 agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata gtattccagt
     2401 agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca aaaaagcata
     2461 tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat gaatccatat
     2521 gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt aaaaagagaa
     2581 gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata tgtggtggga
     2641 aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt attttagtat
     2701 gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc ctttgatttt
     2761 acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg acactgtcga
     2821 tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat tgttctttat
     2881 gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt tccttgactt
     2941 tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac atgatttttt
     3001 acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa ataacgatca
     3061 tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc tccaacttgg
     3121 gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga ggtagaaaga
     3181 tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg ctatttggac
     3241 tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat cagctaaatc
     3301 cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa gtaaatgaat
     3361 atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa cgaacggttg
     3421 aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc aataaaggag
     3481 cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa gcattagcaa
     3541 ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt cataatttaa
     3601 ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca aaaagattgt
     3661 tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc agggttcgaa
     3721 cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc ccatttattc
     3781 aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg atcggatagt
     3841 tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc aggaagggca
     3901 atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac aggttcattc
     3961 ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata ctctgttcga
     4021 gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc gtaataaaca
     4081 agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct gcgacgctcg
     4141 ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg gacaaaaata
     4201 atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat ctacgctccc
     4261 ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag cttttctcca
     4321 aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg atcggtactg
     4381 ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca tatccagtga
     4441 ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg gtccaagtca
     4501 taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag acaggatttc
     4561 ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga aaagtgtgat
     4621 aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag acgaccatag
     4681 cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca tgagtcccat
     4741 ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta gttgtttcat
     4801 aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt tctaaagaag
     4861 gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc tcttttgtct
     4921 ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct ggagaatgga
     4981 tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct agaaacaata
     5041 gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgt
//
LOCUS       DANKHK010000130         5070 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000130 DANKHK010000000
VERSION     DANKHK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            29..982
                     /locus_tag="PW109_002398"
     CDS             29..982
                     /locus_tag="PW109_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDL2512176.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(1380..2354)
                     /locus_tag="PW109_002399"
     CDS             complement(1380..2354)
                     /locus_tag="PW109_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2512177.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            2469..2903
                     /locus_tag="PW109_002400"
     CDS             2469..2903
                     /locus_tag="PW109_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2512178.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3028..4050)
                     /locus_tag="PW109_002401"
     CDS             complement(3028..4050)
                     /locus_tag="PW109_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL2512179.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            4442..4864
                     /gene="arsC"
                     /locus_tag="PW109_002402"
     CDS             4442..4864
                     /gene="arsC"
                     /locus_tag="PW109_002402"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDL2512180.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1577 a          996 c          913 g         1584 t
ORIGIN      
        1 tacttaacaa tattaaagga gcccaaaaat gatactgaca atcacgatga acccatccat
       61 tgattccatt tattttacaa aacactttga attaggtacg atgacacgct ttgattctcc
      121 ggtacgctat gtaggcggca agggaatcaa ttgcggacga gcttctgctc tactaggttc
      181 atcagttatt ttaacagggt ttttgggcgg ttattttggc agtatgattc aagatatgct
      241 gaaaaatgaa aatttgtttg atttgcattt tttacctatt gcagaagaat caagaggtgc
      301 cgtcacgatc atgcatgatg atgaaacgca gacagagcta gtggaagctg gtcctgaaat
      361 agcagatact actatcaaaa aattaatgaa tgatatcaaa ctaatcagta aaaataatta
      421 cctttcagtc attacaataa atggttctgt taacaatcca gatccctttt tgtatgctaa
      481 attattacaa tatatcaaag aacaaataga tccttccacc tatatattaa tggatatatc
      541 tggacaacat ctccgtgaag tcttagcaag taagtataaa ccgatgttca tcaaacctaa
      601 tatccatgag ttttcagata tcatccatca aaaaattact caaaaaagcg acataaaaca
      661 ccatctaaaa acaaatccac tatttaaaga tatcgatgtt atcatgattt cttgcggctc
      721 gtatggtgca gtagtcaaag taaaagaaga attttggaat gttgagatcc ctaagattcc
      781 tatcattaat acaacaggct ctggggatgc cactgttgga ggttttgctc attcattaga
      841 acagaattat cctttaaaag aatgtgtaag gtacgcaatg gcttgcggca tgtcgaatgc
      901 acagcaatca gaagtcggga aagttattcc agctgatgta aaaaatttag caaaaagcat
      961 taaaatcaca gaaatgaaat aaatagtaca aagtaaataa ttgtttatat gaaatgagac
     1021 acaaagagac tgtgacaaaa aacgtgtgta ccgaccccca aaagttagat tttttggtct
     1081 aacttttggg agtcgataca ttgtcacagt ccttttttcg aataaacggt gttctcctgc
     1141 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctattt tttgaaattg
     1201 ctccttgtga ctcagagtta aacggcaggc tcacgacctc ttggtataaa cggtgttcaa
     1261 aagcttgcat ctacgctaat gagtccaacc aagtcaaaaa tgaggagctt ttttggctgt
     1321 ccttattact tgatgcaggg cagttttttc ataatctctt ttagattaaa tccttttggt
     1381 tacacaaggt tctcttcaaa gaatgttgcc attttttcaa atggaatgat gtctgtctgg
     1441 tcgtatagat ctgtatggga tgctcctgga atgatcatga gttccttgtt gtcacctttt
     1501 agttctttga aagcgtcctc cccaaagtaa cgtgaatgag ctttttcccc gtgaatgact
     1561 aagactgcat gacggatttc tggaatataa tgcaatagtt ttgcattcat aagtgacaac
     1621 cctgatgtcc tattccaacc atcattggag tttaaagaac ggacatgata tccgcggtct
     1681 gttttataat acgcatgata gtctttgaca aattgaggag catcttcagg caacggatca
     1741 accacacctc cagcacgtgc gtagtcccct tgtttgaaat caatgatgcg ttgtgcattt
     1801 aattcttcac gcatttcata tcgggcattt tcatccataa catcgaagta gccatttgct
     1861 gttactcgag tcatgtcata cattgtggaa acaactgttg ctttgacccg cgtatcgata
     1921 gctgctgcat tcaatgccat acctccccat ccacaaatac caatgattcc gattctctca
     1981 gggtttacat ttttttgtac agataaaaag tctactgccg cttggaaatc ttccgtattg
     2041 atatcaggcg aagcaacata tcgaggtact cctccgcttt ctcctgtaaa cgatggatca
     2101 aacgcaatcg ttaaaaagcc tctttcagcc atcgtctgcg catatagacc agacgattgt
     2161 tccttcactg caccaaatgg cccgctcaca gcgattgccg gcaatttctt ttcacttact
     2221 tcttttggtt catataaatc agcgactaat gtgataccaa aacgattcgg gaagctcacc
     2281 tttcgatgat tgacttgatt gctttttgga aatactttgt cccattcttg tgtgatgttt
     2341 aattctttat tcatattcta gactctcctt tgatcaaaat tgacatttgt ttttgaatgt
     2401 attattatga taaaacttaa acttgacttt aggtcaaggt aaaaatttta ttttaggagg
     2461 atttttttat gtattccatt ggacaagtgt ctgaaatgtt tggattgcct gtttctactt
     2521 tacgttatta cgacaaagag ggattatttc ctgatatcca gcgtgtttct ggtattagaa
     2581 aattttcaga gaatgagtta gaggctttaa gagtcattga ctgtttgaaa aaatctggtt
     2641 tagaaataaa agacattaag caattcatgg aatggagcaa agaaggcagt aagacatttg
     2701 aggttcgtaa agaactattt gaaaaacaaa aagaagtagt agaaaaagaa atagcaaaat
     2761 tagaaagagt actcgctatg ctaaactaca aaagctggta ctatgaagaa gcaataaaag
     2821 ccggcaatga agaagccgta ttgacaatga ttcctgatga tttaccccaa catgtaaaag
     2881 aagcttatgt acattctcat tgaacaaacg gataactcac tctccttctc tatgaacttc
     2941 atgaaaacat gaataaacac ctctaactga ttgttagaag tgtttatttt ttcactcaag
     3001 tatcctgcac ttacaaaaat atccttttta ccgccatcct aattctggag cgatatcttt
     3061 tacgattgtt tcaagcaagt gcatgttata ttctaccccc aaagtattag gaatcgtgac
     3121 aagtactgtg tctgcttctt tgattgcttc atcttcagct agctccttga ttaattgatc
     3181 aggttcacca gcaaaagttt ttccaaaaat cgttgggttt cggtgataag caagatagcc
     3241 gacagcatct tgttgcggac taccgtcttg tccaaataag cgtcgatcca aatcagtggt
     3301 gattggctga atcgagcgag taactaatgt tcttggttca aaatcatgac ctgcttgctt
     3361 ccacgcttct ttatacgcac gtaattggtt tgcttgttgg atatggaatg gctcgtggga
     3421 ttcgtaattg actaatgtag atgattgcag attcattcca tgttgtgctg cccagattgc
     3481 cgtttgctgc gaaccagctc cccaccagat tctttgtcgt aatccttctg aataaggttc
     3541 tatccgtaat tttcctgatc cttgtgggaa cattggttga ggatttggtt cttcaaatgg
     3601 ttttccttca agtaattcca agaattcaag ggtgcgttcc cgtgttacct ctttgatttc
     3661 ttcttcactg taatcataac caaagtattt ccagccatct agaacttgtt caggtgaacc
     3721 acgccccact cctagttgca ctcttccttg cgtgatgata tctgtcaatc cggcattttc
     3781 tgccatataa taagggtttt catatcgcat atcaataagt cctgtaccga tttcaatctt
     3841 acttgtcttt gcgccgattg ccgctagcaa aggaaacggc gacccaatct gaggggcaaa
     3901 atgatgtacg cggaaaaacg ctccgtccac tccgattttt tctgcttcca ccgctaaatc
     3961 aatcgattgc aaataagcat cttgagctga tttcacaagc gatccttgct ccatccaatg
     4021 tccaaagctt aaaaagccga tttttttcaa ggtaatgacc tcctttaaac ctacaaaaat
     4081 ggaaggtttt ttatttttaa tatgaactga ttctactact ttttttctta aaagacgaat
     4141 aattgttctc gcctcacaat caagaaatgt atcggcttac tacaccccta ctttcttcat
     4201 catctgccgt gtcttaatct cttttaacag tctgttacat cgttgatttt tgactcaaaa
     4261 tgattgtttc taacatgtat ttatggtaaa aaagacaaga ttagttcttt gtacacatcg
     4321 atttatacgc tgaacaaaaa gaaatatata aactaagaaa cccattgttt tttcgctcca
     4381 tcacatagag tttactttag ataaacagtg acatttacta atccataacg gaggaaatca
     4441 aatgaaaaaa atctatttct tatgtacggg caattcatgc cgcagtcaaa tagcagaagg
     4501 ctacgcgcgt aaatacttgt ctccttcaat gtttgagata agaagtgcgg ggatcgagac
     4561 acatggcttg aatccaagag ctataaaaat catggcagaa gacggcatcg atatttccaa
     4621 acagacgtct gatttgattg atagagatta ctttactcag gcagacttga tcattacact
     4681 ttgtggtgat gccaaagaca agtgccctgc cgttccaaac aatcacgaac accttcactg
     4741 ggacctaaaa gatcctgcac aagctgtcgg gagcgaagaa gaaatattaa cggaattcag
     4801 aaaagtcaga gaaggaatca aaagaaaagt tttagactta aatcaacgta catcgatcgt
     4861 ataaagaagt attcaaaatg atttttcaag cactaagaaa caatgttaaa aaaatagctt
     4921 tccatatttt acaaaattgt gccttattgc cgacaaattg tctcttgaac accgtttacc
     4981 agtagtcaag acatcgtaag caactataaa aaattataag aaactcaaaa taaaaggatg
     5041 tgccacaact ttggccacat cctctttttt
//
LOCUS       DANKHK010000131         5066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000131 DANKHK010000000
VERSION     DANKHK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(230..2197)
                     /gene="optrA"
                     /locus_tag="PW109_002403"
     CDS             complement(230..2197)
                     /gene="optrA"
                     /locus_tag="PW109_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein OptrA"
                     /protein_id="HDL2512181.1"
                     /translation="MSKATFAIASTNAKEDMEMQYKIINGAVYYDGNMVLENIGIEIN
                     DNEKIAIVGRNGCGKTTLLKAIIGEIELEEGTGESEFQVIKTGNPYISYLRQMPFEDE
                     SISMVDEVRTVFKTLIDMENKMKQLIDKMENQYDDKIINEYSDIFERYMALGGLTYQK
                     EYETMIRSMGFTEADDKKPISEFSGGQRTKIAFIKILLTKPDILLLDEPTNHLDIETI
                     QWLESYLRSYKSTLVIISHDRMFLNRIVDKVYEIEWGETKCYKGNYSAFEEQKRENHI
                     KQQKDYDLQQKEIERITRLIERFRYKPTKAKMVQSKIKLLQRMQILNAPDQYDTKTYM
                     SKFQPRISSSRQVLSVSELVIGYDTPLAKVNFNLERGQKLGIVGSNGIGKSTLLKTLM
                     DGVAALSGDFKFGYNVEIGYFDQQLAQISGDDTLFEIFQSEYPELNDTEVRTALGSFQ
                     FSGDDVFRPVSSLSGGEKVRLTLCKLLYKRPNVLILDEPTNHMDIIGKENLENILCSY
                     QGTIIFVSHDRYFTNKIADRLLVFDKDGVEFVQSTYGEYEKKRMNSEKPFNNIKVEQK
                     VEKNNTVKGDRNSIEKEKVKKEKRIEKLEVLINQYDEELERLNKIISEPNNSSDYIVL
                     TEIQKSIDDVKRCQGNYFNEWEQLMRELEVM"
     gene            complement(2529..2699)
                     /locus_tag="PW109_002404"
     CDS             complement(2529..2699)
                     /locus_tag="PW109_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512182.1"
                     /translation="MGDRFSPRYAKFLLVYTNQSISEISEYLIFNSQSYFTSVFKKET
                     GKTPFEYRKSDL"
     gene            complement(2885..4312)
                     /gene="fexA"
                     /locus_tag="PW109_002405"
     CDS             complement(2885..4312)
                     /gene="fexA"
                     /locus_tag="PW109_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FexA"
                     /protein_id="HDL2512183.1"
                     /translation="MKKDSKSKEMIQSEKRGSTRLLMMVLSLSVLVGSITADSVNPVL
                     PLISKALEASKSQVSWIVSGIALVLAIGVPIYGRISDFFELRKLYIFAIMILASGSLL
                     CAIAPNLPLLVLGRMVQGAGMSAIPVLSVIAISKVFPQGKRGGALGIIAGSIGVGTAA
                     GPIFGGVVGQYLGWNALFWFTFLLAIMIVIGAYYALPTIKPAESVGSNKNFDFIGGLF
                     LGLTVGLLLFGITQGETSGFSSFSSLTSLIGSVVALVGFIWRIVTAENPFVPPVLFNN
                     KDYVNTVIIAFFSMFAYFAVLVFVPLLVIEVNGLSSGQAGMILLPGGVAVAILSPFVG
                     RLSDRFGDKRLIITGMTLMGLSTLFLSTYASGASPLLVSVGVLGVGIAFAFTNSPANN
                     AAVSALDADKVGVGMGIFQGALYLGAGTGAGMIGALLSARRDATEPINPLYILDAMSY
                     SDAFLAATGAILIALIAGLGLKKRG"
     gene            complement(4566..4709)
                     /locus_tag="PW109_002406"
     CDS             complement(4566..4709)
                     /locus_tag="PW109_002406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512184.1"
                     /translation="MGIDFINVEFRKHVKVSGDLGTTGSVCPFSQKQKMETLPENFLP
                     PFI"
BASE COUNT         1633 a         1059 c          736 g         1638 t
ORIGIN      
        1 tggaagaaca acttctggca gcataacaat caaaggaaat ttagtgagtt tatacgacta
       61 cacttttctt gacaattcca aagtatagat aataaaaacc gctatgcggc aaactcaaaa
      121 ggtcttcaca tagcgattat atctaataaa aattcattaa tttttttaca ttttcaacaa
      181 ccagaatgta tcattaacac ttttacatcc ttattgcata actcaaaaat tacataactt
      241 ccaattctct catcaactgt tcccattcat taaaataatt accctgacac cttttaacat
      301 catcaattga tttttgtatt tccgtcagta ctatataatc agaagaattg tttggttcag
      361 aaatgatttt attcaatctt tctaattctt catcatattg atttattaac acttcaagct
      421 tttcaattcg tttctccttc ttaaccttct ccttctcaat ggagttacga tcgcctttta
      481 ctgtgttatt tttctctact ttctgctcaa ctttaatgtt attaaatggc ttttcagaat
      541 tcatcctttt tttctcgtac tcaccataag tagattgtac aaactctaca ccatccttat
      601 caaaaacaag taatctgtca gcaatcttat tagtaaaata tctatcatgt gacacaaaaa
      661 taattgtacc ttgataactg cataagatat tctctaaatt ctcttttcca ataatatcca
      721 tgtggtttgt cggttcatct aagattaaaa cattaggacg tttatataat aatttacata
      781 atgtcaatct aaccttttct ccacctgaca aagaggacac cggtctaaaa acatcatctc
      841 cactaaactg aaatgagcca agagcagttc tgacctctgt gtcatttagc tcagggtatt
      901 cgctttgaaa aatttcgaat agtgtatcat ctccactgat ttgagcaagc tgttggtcaa
      961 aatagccaat ttcaacattg tatccgaatt taaaatctcc agacaatgct gccacaccat
     1021 ccataagtgt tttaagcaac gtggatttac caataccatt actcccaaca attccaagct
     1081 tctgtcccct ttcaaggttg aaattaacct ttgcaagagg agtatcatag cctatcacaa
     1141 gttctgaaac acttaatact tgccttgaac tactgattct cggttgaaat ttagacatat
     1201 aagtttttgt atcgtattgg tctggtgcat ttaatatttg catacgctgt aataatttaa
     1261 ttttagattg caccatttta gctttcgtag gtttataacg aaaacgttca atcaagcgtg
     1321 taatcctttc aatttctttc tgttgcaagt cgtaatcttt ttgctgtttg atatgatttt
     1381 ctcgtttttg ctcctcaaag gctgaataat tacctttata acatttggtc tctccccatt
     1441 cgatttcata aaccttatcc acaattcgat taagaaacat tctatcatgg gaaataataa
     1501 ccaatgtaga tttataactt ctcaaataac tctccaacca ttgtattgtt tctatatcaa
     1561 ggtggttagt aggttcatca agtaatagaa tgtctggctt tgttaaaagt atttttataa
     1621 aagctatctt agttcgctga ccacctgaaa attcagaaat gggtttttta tcatctgctt
     1681 cagtaaaacc catactacga atcatcgttt catattcttt ttggtaggtt agacctccaa
     1741 gagccatata cctttcaaag atatcagagt attcattgat gattttatca tcatattgat
     1801 tctccatttt atctatcagc tgtttcatct tgttttccat atcaataagc gtcttaaata
     1861 ccgtacggac ttcatccacc attgatatac tttcatcttc aaaaggcatc tgtcttaaat
     1921 agctaatata agggttaccg gtctttatta cttgaaactc actttcacca gttccttctt
     1981 ctaattcaat ttcgcctata atagctttta gcaaggttgt ttttccacat ccatttctac
     2041 caacaatagc aatcttttca ttatcattga tttcaatacc gatgttttcc aacaccatat
     2101 taccatcata gtaaacggca ccattaatta ttttgtattg catttccata tcctcctttg
     2161 cgttagtact agcaattgca aaggtggctt tggacaataa ttattctact ccatgtaaag
     2221 cttgtcacag ccacctatac atggagttct ttaaatgtta ctatagttgt agatttcatt
     2281 tgcttctacc tcctatcgat tattaatata attcaattgc caccttattg taccaagtcg
     2341 ctagcgcaat ggctagccta agttttagct tacacccttt ctggtaaaag taaagtaact
     2401 gatctgttat ctcatctggc accagattcc cgataaaccc tgctgttccc gtcaacagaa
     2461 accgcagcca cactggaaaa agatacaaaa ggattgccgc aattgattct ttgcattagc
     2521 catcagcttt acaaatcact ctttctatat tcaaaaggcg tttttcctgt ttctttttta
     2581 aacacggatg tgaagtaact ttgtgagtta aatataagat attcactaat ttctgatatt
     2641 gactgattcg tatataccaa tagaaatttg gcatatctgg gtgagaaacg atcaccaatg
     2701 ttttcattgt ttcatcctta acatactcaa ctttcgcacc aataaaacag tgtacgaaag
     2761 ttgagttact taaaagtatt ctattctaag aaattcaaat ttgcgtgatt cctaagctat
     2821 ttatttacag cttaaactat agaagatgaa ctaaacaata actaatcttt acataactaa
     2881 gttattaccc acgctttttt aaacctaatc cagctattaa ggcaatgagt attgcccctg
     2941 tagctgcaag gaacgcatct gagtaggaca tagcgtccaa tatatataat ggatttatcg
     3001 gctcagtagc atcacgtcga gcggataata atgctccaat catacctgct ccagttcctg
     3061 ctccaaggta caaagcacct tggaaaatcc ccattccgac accaaccttg tctgcatcga
     3121 gtgcacttac tgcggcgtta tttgcgggag aattcgtgaa tgcaaaagca atccctactc
     3181 cgaggacccc cacggaaact aacagaggtg aagcaccaga tgcataggtg gacaagaata
     3241 aggtagacag ccccatcaga gtcatcccag taattatcag acgtttatcc ccaaatcgat
     3301 cagaaagacg gccaacgaag ggagataaga ttgcaacagc cacaccacct ggcaacaata
     3361 tcattccagc ctgtccagaa gagagtccat tcacctcaat gactagtaat gggacgaaca
     3421 caagaacagc gaaataagca aacatcgaaa aaaatgcaat tatgaccgta ttgacataat
     3481 ccttgttatt gaacaggaca ggtggtacaa atggattttc tgcggtaaca attctccaaa
     3541 taaatcccac caaagctaca acagaaccaa ttaggctagt taacgatgag aacgaagaaa
     3601 aaccagaagt ttctccttga gtgatgccaa aaaggagtaa tcctactgtg aggccgagga
     3661 ataaaccacc aatgaaatca aagttcttat tgcttcctac ggattctgcc ggtttaattg
     3721 tcggtaacgc gtagtaggca ccaataacaa tcataatggc taacaaaaat gtgaaccaaa
     3781 acaaggcatt ccaccctaaa tattgaccaa ctactccacc aaatattgga ccagcagcag
     3841 ttccaacacc aatacttcct gcgataattc ccaaagctcc cccacgtttt ccttgtggga
     3901 aaaccttcga aattgcaatg actgatagaa ctggaattgc ggacatccca gcaccctgaa
     3961 ccattcttcc caaaaccaac aatgggaggt tcggggcaat tgcacataaa agactaccac
     4021 ttgccagaat cataatggca aagatatata gctttcgtaa ctcaaaaaag tctgagattc
     4081 gaccataaat cggaactcca atcgcaagaa caagtgcaat accactaact atccaactca
     4141 cttgagattt cgaagcttct aaagctttgc ttattagtgg aagtacggga ttgactgaat
     4201 cagccgtaat tgaacctaca agtacagata gggagagtac catcattaaa agcctagtag
     4261 aacccctttt ttcagattga atcatttctt tagatttact atcctttttc attttatttc
     4321 ctcctaaaat atattgtgat tttaggaggc taaatccact gctttcacca taaacctcac
     4381 ctcatatcta tttatattgt aaaaccaaat caacgaactc accatatttc tacagcatga
     4441 ttaaagattc ggttctccaa actaactgaa ctgatcaaac aaaaaaaagg caccagttta
     4501 acaaccggtg cctaatcatt cgtatgcatt tttacaaatg caatagattc ataaattttt
     4561 gcccattata taaaaggagg caaaaagttt tccggtagtg tttccatttt ttgtttttgt
     4621 gaaaaaggac atactgatcc cgttgtacct aaatcccctg aaacttttac atgctttcta
     4681 aactcgacat taatgaaatc aattcccatt actttttgcc tcctttctca tattgtgata
     4741 ataacacaat tataattgat gaaaaagaaa taatcaaccc tacttttttg aaaaccaatt
     4801 taaagcttta ctacttttac ttacataata aaggcaggtg cagtataaac ttttttaaat
     4861 aggtcttctt taacgagcag gtagcgtcag ttaaagtaga taatttaaga tcgaaatcat
     4921 attatagata ttccgaaatt gggcgtgatt gttgcaggtc aaaagcttaa ttcctcgata
     4981 aaataaaaag gaattagttt ttttatttgt ggtacattct tctgaccgta atcaattgtc
     5041 atctaataat tacattgtat tttttc
//
LOCUS       DANKHK010000132         4990 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000132 DANKHK010000000
VERSION     DANKHK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..226
                     /locus_tag="PW109_002407"
     CDS             <1..226
                     /locus_tag="PW109_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDL2512185.1"
                     /translation="KKSFYGCSNYPECKFTLAEHFRKKKLTKTNIKELLEGKETLIKG
                     IKNKEKKSYNAVVKIGEKGYIDFISFSKQA"
     gene            353..454
                     /locus_tag="PW109_002408"
                     /pseudo
     CDS             353..454
                     /locus_tag="PW109_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            457..1806
                     /locus_tag="PW109_002409"
                     /pseudo
     CDS             457..1806
                     /locus_tag="PW109_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
     gene            2203..3693
                     /gene="abc-f"
                     /locus_tag="PW109_002410"
     CDS             2203..3693
                     /gene="abc-f"
                     /locus_tag="PW109_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDL2512186.1"
                     /translation="MTTIEIKNLTFGFDVQTTNLFEQTNLTIQSEWKLGLIGRNGRGK
                     TTLLKLLMGEYTYSGKIEHQLNFTYFPQKVQDKTQLTYYVLQELSDFEQWKIERELNL
                     LQVDSDILWRPFHSLSGGEQTKVLLALLFVDDVNYPLIDEPTNHLDVMARQQVAAYLK
                     AKKQGFIVVSHDRSFVDEVVDHVLSIEKSQLVLYQGDFSVYEEQKGLRDQFEQEQNVK
                     LKKEIGRLKQTAAEKAEWSRGRERDKLGSPNKPGSGAIYDTGAIGARAARTMKRSKAI
                     VKRMEDQATEKEKLLKDMEYIEPLSMNYQVTHRKKLLTVQELQLSYGERKLFSPLTFD
                     INQGDRLAIQGPNGSGKSSVIQHLLGIFQGEVTGEVIQPQGVTISYVRQNYEDNRGTL
                     QEFAEEHHLSYQELLNNLWKLGVERKVFQNRIEEMSMGQRKRVELAKSLATPAELFIW
                     DEPLNYLDVFNHKQLEEVIQLVQPTMLIVEHDRTFLNKVATKIIPL"
     gene            complement(3770..3931)
                     /locus_tag="PW109_002411"
     CDS             complement(3770..3931)
                     /locus_tag="PW109_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512187.1"
                     /translation="MDQLDVLDRLKEELEIPFLQIKLQDKTYSEEEYQQLKTELVQYF
                     DDYVRNVEN"
     gene            complement(3951..4301)
                     /locus_tag="PW109_002412"
     CDS             complement(3951..4301)
                     /locus_tag="PW109_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512188.1"
                     /translation="MFSKTPVELLVKDFSNIYNKCQSAYELVSSHRYNEFLAILTTAE
                     AYAIAEKAYVRCDTFQELQTPEVEEFVNAFDIFYFELKQVLFHKNDDLDSLYNRLNTM
                     SEVYQKVNNSFDLI"
     gene            complement(4318..>4990)
                     /locus_tag="PW109_002413"
     CDS             complement(4318..>4990)
                     /locus_tag="PW109_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2512189.1"
                     /translation="LQLTDAIFHDEPVGPHENQDRAHAHYSDLTPDNRLIVCDLGTDR
                     VYTYDVSDEGKLTEVATYLAPAGTGPRHLVFHPNTHYAYLFGELDSTVTVLSYEASTG
                     TLTELQKLSTLPEDFEGFNGGAAIRISSDGRFVYLSNRGHNSIAVFAISEDGAKVTFV
                     QHISTEGDHPRDFNLDPTEKFVVCANQNTDNLTLYRRDAENGTLTLIKKDIYAPECVC
                     VLFEK"
BASE COUNT         1738 a          799 c         1034 g         1419 t
ORIGIN      
        1 aaaaaaatcg ttttatggtt gttcaaatta tcctgaatgt aagtttactt tagctgaaca
       61 ctttagaaag aaaaaactaa ccaaaacaaa tataaaagaa ttactggagg gaaaagaaac
      121 cctgataaaa ggaatcaaaa ataaagagaa aaagtcctac aatgccgttg taaaaatcgg
      181 ggaaaaggga tatatagatt ttatctcatt ttcaaaacaa gcataaaagc cctttaaata
      241 gggcttttat atattaatca caaatcacaa gtgattaatc acaaatcact tgtgatttgt
      301 gattgttgat gataaaataa gagtaagaag aaatagaaag aagtgagtga ttgtgggaaa
      361 tttaggcgcg caaaaagcaa aacgaaatga tacaccaatc agtgcaaaaa aagatataat
      421 gggagataag acggttcgtg ttcgtgctga cttacaatgg tgatgttctt tgcgcaatta
      481 attcaactga tggttcaatt agcttttcaa caattggatg aagaagtgtc ttcagaattg
      541 aaaaaagaag ggttccaagt tgatcgaaaa agccaacgaa ccgttacttt tctatttggt
      601 tctgtcactt atgtgagacg aagaatgaag aataaagccg gtgaaatccg ctatcccctt
      661 gacgaatttt tagggatacg caaaggaatt cgctatagtt cactggtctt aagaaatgtc
      721 tctgaattag gtacaatgat ggtgtatcgt cacgtgtcaa aagcgattga ttgcttaact
      781 acctggagta tgagccatca aaacgtccaa caattggtgg tgaaaacagg agaaattgtg
      841 cagaaacgaa gtacgcatga aagtcgatat gacgaagtaa aacaaaagaa aaaagttcct
      901 tacttgtatc tagaaggtga cggtgtgagg attcggggac agaaaaaaca acacctagaa
      961 attcaccgtt ttcaagtgag tgaaggtagc caaaagatag gcaaaagatc ccagttaatt
     1021 tctccttatt ttgtcagtca tttgaatcga aaaaatgccc aaaaagagat gatgaattat
     1081 cttcaagcaa cctatgattt aacagagaca atcgtgatat ccaacagtga tggtggttct
     1141 ggctatgaaa aagaagtctt tgaggaactt tctttgggct gtttccgtca tgaacatttt
     1201 cgtgacatgt atcatgtgca tcgaaaaata aaagagcgac taaattttgt ttcatctaag
     1261 ctacaaggaa aattaataga agcgattcac cagtacgaca aagaaaaagt acgtgcttgt
     1321 ttggacaccg cagaaagtct gattgaggag cgagaagaaa cctgtttgga acagttgagg
     1381 ttattgcaag gctatcttga tcggaattgg gtgtatttac aatctcttga aaaaagaggg
     1441 ttctatgacg ataagcaaag tatcggaacc attgaaagta cgcatcggaa aatgacctat
     1501 cggatgaagc gacaaggtcg tttgtggagt agaacaggag cggcagccat gattcgtgtg
     1561 atagatagtt tgaggaatca agaattgaat acttggctaa atcaatatga agaagtggcg
     1621 gatgacgaat tagaacgacg aaaacaatgg aaagcaatga aacgaaaagt cctaaaagcc
     1681 cctgcttttc aatcacatga aggagcattt aaagggagca ttggtcaggg acttgcgaaa
     1741 agcgcaccaa taggtcaact atcaaaagga ctgaagcaat taaacatgac ccctagttat
     1801 ttctaatgga aaaaattttt ccattagaaa taactaggca aaccgaacgc aagtgaggta
     1861 ctaataacaa aatttaagtg aagtcagctg cttttttatt caacctcaaa aaatttgaca
     1921 catacaaaat caagaaatca tttgctttct aatccaattt atgctattct tatactaatc
     1981 aaaaacgaag tgagggatcc tctattgaaa aattaagcca atgtgttgaa gctgcattca
     2041 gactattttc tttgaagtac aagggaatag tgtgcagatt ttcaggattg gtgattattt
     2101 ttcgataaga gggtcttttt gtgtcttcct atctaaaaaa tctctgtcct gaaaataggt
     2161 gagggatttt tcttgcctaa tcggtgagtc gagggaattt aaatgacaac aattgaaata
     2221 aagaatttaa cgtttggctt tgatgtccaa acaacgaact tatttgaaca aacgaatctc
     2281 accattcaat cagaatggaa attaggatta attggccgca atggtcgtgg caaaacgaca
     2341 ctactaaaat tgttgatggg tgaatacacg tatagtggga aaattgagca tcagttgaac
     2401 tttacttatt ttccacaaaa agttcaagat aagacgcaat taacgtatta tgtgctacaa
     2461 gaactctctg attttgaaca atggaaaata gaacgagaac tcaacctcct acaagtagat
     2521 tcagatattt tatggcgacc gtttcatagt ttatcaggtg gagagcaaac caaagtattg
     2581 ttagccttgc tttttgttga tgatgtgaat tatccattaa ttgatgaacc aaccaatcat
     2641 ttggatgtaa tggctcgtca acaagtggcg gcgtatttaa aggcgaagaa acaagggttt
     2701 attgttgtta gccatgatcg aagttttgtg gatgaagtgg tggatcatgt attatccatt
     2761 gaaaaaagtc agctagtgtt gtatcaagga gatttttcgg tctacgaaga acaaaaaggc
     2821 ttacgtgatc aattcgaaca agagcagaat gtgaaactca aaaaagaaat tggacgctta
     2881 aaacaaaccg cagcagaaaa agcagagtgg tcacgtggga gagagcgaga taaattgggc
     2941 tcgcccaaca aaccgggaag tggagccatt tatgataccg gtgcgattgg tgcgcgagct
     3001 gcgcgaacga tgaaacgctc caaagcaatc gtcaagcgta tggaagatca agcaacagaa
     3061 aaagaaaaat tattgaagga tatggaatac attgagccat taagtatgaa ctatcaagta
     3121 actcatcgaa aaaaattact gactgtgcaa gagttgcagt tgagttatgg agaacgaaag
     3181 ctattttctc cccttacttt cgacattaat caaggggatc gattggcaat tcaaggacca
     3241 aatggctcag gaaagtcttc agttattcag catcttttag gaatatttca aggtgaagtg
     3301 acaggtgaag tcatacaacc acaaggagta accatcagtt atgttcgcca aaattatgaa
     3361 gataatcgag gaaccttgca agaatttgct gaagaacatc acctatccta tcaagaatta
     3421 ttgaataatc tctggaagct gggagtggaa cggaaggttt ttcaaaatcg gattgaagag
     3481 atgagtatgg ggcaacgaaa acgggtagaa ttagcaaaat ctttagcgac accagcagag
     3541 ctatttatct gggatgaacc cttaaattat ctagatgtct ttaaccacaa gcagttggag
     3601 gaagtcattc aactcgttca accaacgatg ctgattgttg aacatgaccg aacctttttg
     3661 aataaagtag cgactaaaat tatcccgtta tagtaaaaaa gtaaaataga aaatccttat
     3721 gaaatttgtt ttaagaacat caatttcata aggattcttt catttttgtt taattttcta
     3781 cattacgcac ataatcatca aagtattgaa ctaattcggt ttttaattgt tgatattctt
     3841 cttcggaata cgttttgtct tgcagtttga tctgtaaaaa aggaatttct agttcttctt
     3901 tgaggcgatc aagcacatct aactgatcca tttttttcat cacctatcta ctaaattaaa
     3961 tcaaaggaat tattgacttt ttggtatact tcgctcattg tgtttaagcg attgtacagc
     4021 gaatctaaat cgtcattttt atgaaagagc acttgtttta attcaaaata gaaaatatca
     4081 aatgcgttga cgaattcttc cacttctggt gtttgtaatt cttgaaatgt gtcacaacgg
     4141 acataggctt tttcagcaat tgcgtatgct tcagcagtag ttaaaatagc taagaactca
     4201 ttgtagcgat ggctcgatac taattcataa gcagattggc atttgttgta aatatttgaa
     4261 aagtctttta ctagtaattc gacgggtgtt ttagaaaaca ttgcattcac tcctccatta
     4321 tttttcaaat aagacacaga cacattcagg tgcgtaaata tcttttttga ttaaagttaa
     4381 ggttccattc tccgcatcac gacgataaag agttaaatta tcggtatttt gattggcaca
     4441 aacaacgaat ttctctgttg ggtctaaatt gaaatcacgt ggatgatctc cttcagttga
     4501 aatatgttga acgaaagtta cttttgctcc gtcttcactt atagcaaaaa cggcgataga
     4561 attgtgacca cggtttgaaa gataaacaaa acgaccatcg ctagaaatgc gaatggcagc
     4621 accaccgttg aaaccttcaa aatcttcagg taaggtagat agtttttgta attcagtcaa
     4681 tgtaccagtt gaagcttcgt aagaaaggac ggttactgta ctatctaatt caccaaataa
     4741 ataagcatag tgggtatttg ggtggaagac taaatgacgt ggacctgttc cagccggtgc
     4801 taagtaagta gcaacttccg ttaatttacc ttcatcagaa acatcatagg tatagacacg
     4861 atctgttcct aaatcgcaga caatgagacg attatctgga gttaaatcac tataatgcgc
     4921 atgggcacga tcttgatttt catgtggtcc gacaggttcg tcatggaaaa tggcatcggt
     4981 cagttgtaag
//
LOCUS       DANKHK010000133         4967 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000133 DANKHK010000000
VERSION     DANKHK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(150..815)
                     /locus_tag="PW109_002414"
     CDS             complement(150..815)
                     /locus_tag="PW109_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512190.1"
                     /translation="MNIGEKIRTYRRNCNLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKPPLSLKKALSFFLL
                     VFFLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFGHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLSWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            995..2977
                     /locus_tag="PW109_002415"
     CDS             995..2977
                     /locus_tag="PW109_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2512191.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFILLFVERYNQAFHTDYATNIFE
                     DFENSYSYSVISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEEETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYKFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPLLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            2981..3619
                     /locus_tag="PW109_002416"
     CDS             2981..3619
                     /locus_tag="PW109_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512192.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQEMKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVMHDPYLTKIGNKKIML"
     gene            3994..4905
                     /locus_tag="PW109_002417"
     CDS             3994..4905
                     /locus_tag="PW109_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2512193.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYNTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         1703 a          763 c          915 g         1586 t
ORIGIN      
        1 atactagaat agtcagtcat tttttttacc tatcattctg aactttttta tttaggaact
       61 ttcatctcaa agctttttca attaatttgt acccctgttt tttttgataa gatcatgtgc
      121 attgttctat ctctacgttc atctcctact taatgcctat ctggctcctg agggcttctt
      181 tttaaggcgt cgtcatagtc ttggctgagg atttcttggt aatctgaggg aatggcttcc
      241 caagagagca ggcgaacgtt catgacgtgt tttcctttgt gtttgattac agcatgattc
      301 ttaccttttt ctacctctcc agcgtgaaaa gagagccatg taccatgacc aaagatatca
      361 ttacataccc aatgttcgat ggaattttcc acgtatccta cagggagtgt ggtatagccg
      421 atttgtggtt tcaaaaaagg attgaatcga tcaaccgttt catttttgta ttgataattg
      481 ataagaaaaa gatagaccaa aaaaaagacg agaagaaaga aagatagtgc ctttttgagt
      541 gataatggtg gttttgatga ataagatttc ttgttaacaa gaggttcgtc tggcaatgtt
      601 ggttctattg attcggtagc tgattcaata gaaggaaaat caatcggcag acctaaagtc
      661 tgatgtaaaa gtgacagcat ttcaagatct ggcattgtac gagaattttc ccaactagaa
      721 atcgttttat cggaaacacc aattttttcg cctaattctt tttgcgtaag gttacaattt
      781 ctgcggtaag ttcgtatttt ttctccgatg ttcatcttct tctcctttcg attctacagt
      841 atatagattt attctacatg acctatacac ttgatagata cgtttttttg atgaaaaacg
      901 gtatgatttt atcaaagaat catttaagta agggagagtg gtgataatca aaaaaaagtt
      961 cacagtttta ctcacaatca tagtgaccgg tatcatgagt ggttttgcct tggttagtat
     1021 aaataaacag gtactctatc ggacaaatga atttttccat caaacagata cttatactcc
     1081 gattcgattg ccacaacatt ttattgggca agaagaatat gagcagctaa tcatggtatt
     1141 tgaaaatgtc tcatcagaaa ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa
     1201 tgaagtagat acatcttgga acttcaagat acagccaaag cttgaaatga cattgtatta
     1261 tcaaggggat gtcaaatctt ctcacgagaa ggtaccctat tctaatacct cctttcgcgt
     1321 aacgtccatt gaagaatcga tcaaaataaa agattttgaa ggtgaatttt tcttgatgac
     1381 gagagaaaaa gaagcctatg aacggttcat tttactattt gtggaacgat acaatcaagc
     1441 atttcacact gattatgcca ctaatatttt tgaagacttt gaaaatagct attcgtattc
     1501 ggttatttca agtacagacg tatataatta tcgagtgtat ttaactattg gattgattct
     1561 tttattcctt ttgttatcat cctttttctt attgatgaat aaagaaatca gtttattgtt
     1621 gaataatggc ttttcattac atcaagtatt gtggtgttta ttggggaaaa agaaaatcat
     1681 tattacattc ataattgcag gaatcattgg tggttttatt tttatgtatt ataaggagca
     1741 atttctttat ttgttccaga tgtacgtttt cttgttcagc ggaatatatg gtatctcttt
     1801 gatttctctt ttattagttg aaaaaatcgc ttcgttaaga gaagcgttca actatatcca
     1861 atacattctt tttacatttg gattgttatt ttctcttctt ttgaccgttg ccaatgtcga
     1921 attagcaact atcgtttttt cttcttcaca gttagctgtt tcccatgaac caccaaaaga
     1981 aatcgctggt gaggactatc atgtatttta tccagtaacc atcgggaaaa accacgtgga
     2041 ttttatttat tccaatcgtt ttgcatcagc agcagatcag gaattatatg aaactttgaa
     2101 taaaaacgga agtatattag tcaatgtatc agattacttg aatgaagaaa acgaacagtt
     2161 tggcagaggg ataaaaataa atctgaatta tcttaagaaa tttcctttga ttgatgtctc
     2221 tggaaggttg atccaaataa ccgaagaaga aacgcaccct gttatattga tccctgaacg
     2281 atatagacat acagatttaa aaaatgacct ggcgcaaata acagaatatt atcaagaaat
     2341 cagtgaaaaa gaaccaaaat ttctatttat aaaaaacaat caacccattt ataaatttat
     2401 tccttctatc ccctggatcg aacattatcc ggttgttgaa atactgacat tgaaaaacag
     2461 tacgtattgg gaaagaaata ttctcagcgg agaaatctat ccactgttaa agatcaagat
     2521 aggctctttg tcgaaagaaa ggttatttga gctgattgaa gaaagtcagt tacgtgataa
     2581 cttgcagtta tcatttccgt atactcagac tgaagagata gctgtcaaag tgttaagcag
     2641 atcatttcat tacttgatac agatattttt actcactttc ttttctttct tcttgttgag
     2701 ctatgtcatg ttatgcatat attttgtcta caattgtcgg aaaatcgcta tctttcgttc
     2761 aagtggttat tcgctctttg aaacgtataa agatttcttc atgatgaacc tcatcaaatg
     2821 gggaacgaca agtgtaatct tcctttttct tatagaaaga gagcctaaat atctttttaa
     2881 tatttttttc tttagcttca ttgattttat tctttcagtt gtttttattt tgtctacaga
     2941 aaagaaaagt cagttattgc tgatgaatgg aggttaacaa ttggagccgt tgatagtgat
     3001 aaaaggtgcg aaaaaaatca ttcgaaacaa aacgattctt catttggatc gtttcactgc
     3061 ggatgctggg gatcttgtga ctgttgttgg agaaagtgga gcaggtaaaa gtacgttatt
     3121 gaatatatta ggattgaatg atcagttcag ttcaggacaa ttctatttgt tcgatcaaga
     3181 gatgaaacaa ttaactggaa gagagaagcg cttgataaaa agaaatacca tatcttttct
     3241 ttttcaagat tttgggttga ttgaatatga aacaatcgat tttaatttag atattggatt
     3301 gaagttttcg aatttaaatg tttcagataa gcgaacagca aagtatgagg cattgaaaaa
     3361 agtaaatata aacaagaaac taagtacgcc tatctacaca ttatctggtg gtgagaagca
     3421 aagaatcgcc tgtgcgagag tcttattgaa gaatagcaaa atcattttag ccgatgaacc
     3481 aactggatca ttagataaac gaaataagga gcaagtcgtt gaaatcttat cgcaacaggc
     3541 ttcccaaggt acgttagtta tcgtggtcat gcatgatcca tatctgacca aaataggaaa
     3601 caagaagatc atgctttaga aaacaaatga aaaatcaatt tttttcaata aaaacaacaa
     3661 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
     3721 agaaaaaaaa attgataaca tttgttctaa gaattagtta cgttaaatac ggaacgcaag
     3781 atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt gctacttgac
     3841 atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc aggacgtatc
     3901 gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg tttttagcaa
     3961 ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt agcacttgcc
     4021 atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa aacagccgtt
     4081 agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa aaaaatgcaa
     4141 ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga taaatatgga
     4201 gatgacgtat caaccaaaga aatcgattcc caatatgatc aaacaaagaa acaattgggt
     4261 gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt caaagacagt
     4321 atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt gaccgatgaa
     4381 gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat tatccaagta
     4441 gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga cgatttctct
     4501 aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg aaaagtgaaa
     4561 tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt taagttgaaa
     4621 gatggtcaag tttctgatgt gatcacttct accaatgctt caacttataa tacggaatat
     4681 tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa atacaaaaaa
     4741 gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca gaataaagta
     4801 attggcgaag tattgaaaga cgctaatgta aaaattaaag acaaagactt tgaaaatgtt
     4861 ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt ataaataaaa
     4921 atatgaacta tttaaatcaa taaaactgtt catctaaatc tttttat
//
LOCUS       DANKHK010000134         4848 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000134 DANKHK010000000
VERSION     DANKHK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(32..2152)
                     /locus_tag="PW109_002418"
     CDS             complement(32..2152)
                     /locus_tag="PW109_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2512194.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2382..3560
                     /locus_tag="PW109_002419"
     CDS             2382..3560
                     /locus_tag="PW109_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2512195.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3576..4334
                     /gene="aroD"
                     /locus_tag="PW109_002420"
     CDS             3576..4334
                     /gene="aroD"
                     /locus_tag="PW109_002420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2512196.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4357..4842
                     /gene="ybaK"
                     /locus_tag="PW109_002421"
     CDS             4357..4842
                     /gene="ybaK"
                     /locus_tag="PW109_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2512197.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
BASE COUNT         1564 a          843 c          994 g         1447 t
ORIGIN      
        1 caaaaagtat agagccttta aatttatttt atcattcgga agaacttgtg gtcgttccgt
       61 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccc ttgtgttcac
      121 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
      181 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
      241 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
      301 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
      361 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
      421 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
      481 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
      541 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
      601 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
      661 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
      721 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
      781 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
      841 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
      901 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
      961 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     1021 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     1081 catggaatac cgcactagtg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     1141 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     1201 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     1261 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcaagct
     1321 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     1381 ggatataaat cacatttttt cctttagcga gcccgaacaa atcatcattt ggtgctgcat
     1441 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     1501 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     1561 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     1621 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     1681 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     1741 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     1801 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     1861 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     1921 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     1981 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     2041 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaaca actagtaaag
     2101 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     2161 tcctttctgc acttcttaat cacagaagtg tcatgaaacc atgttacaca gctcacttat
     2221 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     2281 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     2341 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     2401 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     2461 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     2521 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     2581 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     2641 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga
     2701 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     2761 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatcg
     2821 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     2881 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     2941 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
     3001 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
     3061 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
     3121 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
     3181 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
     3241 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
     3301 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
     3361 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
     3421 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
     3481 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
     3541 aaattaaaaa taaccattag aaggagcgat gcagaatggt ttgggtaaag ggattgaata
     3601 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
     3661 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
     3721 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
     3781 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
     3841 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
     3901 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
     3961 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
     4021 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
     4081 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
     4141 caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
     4201 ctcgtctggc tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
     4261 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
     4321 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
     4381 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
     4441 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
     4501 catatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
     4561 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
     4621 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
     4681 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
     4741 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
     4801 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcata
//
LOCUS       DANKHK010000135         4738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000135 DANKHK010000000
VERSION     DANKHK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            861..1061
                     /locus_tag="PW109_002422"
     CDS             861..1061
                     /locus_tag="PW109_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512198.1"
                     /translation="MKAKIDLFYEKHPYLSLLINLLLGSIIGISVEYLLNKDFIGSGF
                     YTVLFLSVLEAFSIYRKSKKNK"
     gene            complement(1179..2017)
                     /locus_tag="PW109_002423"
                     /pseudo
     CDS             complement(1179..2017)
                     /locus_tag="PW109_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            2202..3089
                     /locus_tag="PW109_002424"
     CDS             2202..3089
                     /locus_tag="PW109_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2512199.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKKVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKAANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKGKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3660..4355)
                     /locus_tag="PW109_002425"
     CDS             complement(3660..4355)
                     /locus_tag="PW109_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL2512200.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQSP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETISELKKVADYF"
     gene            complement(4339..4737)
                     /locus_tag="PW109_002426"
     CDS             complement(4339..4737)
                     /locus_tag="PW109_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2512201.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPYLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1623 a          863 c          711 g         1541 t
ORIGIN      
        1 ttttctttat tctccatgtc tctttttaaa gcttttatgt cgagttttaa taaaatcaat
       61 caattacttc tatactaata gatcaatgtc cacatctttt tgaaccagta ggtaccttac
      121 tacttactat gaaatttctc aagtattaaa aacatcaaaa acatatgctc tgctgcctgt
      181 atcataataa ttaaaataga tacaactaat ttttgtttct ttcgtctaaa ggataatttt
      241 taggcaatac attctgttta ctagcacgtc aaatgacact agcgattatg acttcaaaac
      301 aactgtttgt gattttttct cttttaaaac aagtcacatg ttatattaaa ttcgtaaata
      361 cgtatattac aatttatttt aagaggcgga catcaagaaa aaatattatt aaaaagtttg
      421 ctattatcaa ccttgttact aacttttgga tcaacaattc tcccaactgg aattgttttt
      481 gctagcgaac aaaatatcat caaaaatact agttattcta acgatgataa cttgaatgta
      541 tctatagaaa agacgagaac ggtcgtctta tggtagtaaa aatggaaaca aaagctcgtg
      601 ctaatgttgc aacgtgggaa gcatggcaat atcctcatat tgctttaagt actggtataa
      661 cagctggagc tataaatact gcctactcta gcagctgatt ggacaaatta tggcaatatc
      721 ccagtaaatg cagtcgctcg actataagac aaaaattgta atagctgggt aggattttac
      781 aaacgaactg gctatgacgg agtgagaaga gctgtgacca ctgattataa aatagagtaa
      841 ataatacaaa aggagaacct atgaaagcta aaatagattt attctacgaa aaacatcctt
      901 atctttccct tttaatcaat ttgttgttag gtagtattat cggaatttct gttgaatatt
      961 tactaaataa agactttata ggttctggtt tctatacggt attgttttta agtgttcttg
     1021 aagctttttc aatttacagg aaatccaaaa aaaataaata acttcaaagt ctcttttatt
     1081 agaagggact tttttgttgc ttatagcagg tactaaagta tactctgttg atctccatat
     1141 aacattttaa atatggataa tcattaatag ctatatagtt attgataaac agcagtgtat
     1201 ttcttcatcc gctttgtttt atatgcatag agtaacaaac caacaatcaa gactaacaag
     1261 aataatgtag tcgatataga tgcttcagcc ccaaagtttc ccccagtaat tattgtattt
     1321 tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg ttccgctgac tggtataccc
     1381 aaaatagctc cttgtgtcca attccaagta atatgagcac caatagccgt aaataaactt
     1441 cctgtactgt agataagtgc gctgaaaaag caacctgcta aaaagatatt gaaaactgcc
     1501 agaaatgtca tgttaggatt caatccgtgc aaaagagcaa ataatgcagc agatccaatc
     1561 attgctatta acggaccaaa tctaagcatc tgatgctgaa aaaagccacg gaataaaagc
     1621 tcttctgatg cccccactgc aataaataaa ataaaactcg ttatcagcgg cacaatgttt
     1681 gcgtttcctg aatctaaccg atacgccccc gtcaaatata atggactagt aataagcaga
     1741 aacactccaa ttccaacaac gagacctccc gctaaatacc cccagtcttt tcctcgaaca
     1801 gaaacaccat aatcttctat tctttttcct tcgaccttac tagcaagaaa taaagctagt
     1861 aaaaaacagc taaatatccg actgtaccgg caattgctcc aatccattca ctatttccaa
     1921 taaacatatc cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta
     1981 ttaaaacaga tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa
     2041 gtttaacaac ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga
     2101 gagaatagat ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt
     2161 ctccctaatt cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa
     2221 aacaactact aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca
     2281 agtccaatga aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg
     2341 ataacccaaa agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag
     2401 catttgatga tgtatacgga aaagagattt cttcaaagaa agtaacaaaa gaatataatg
     2461 aacaaatcaa aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag
     2521 aagagacata taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca
     2581 atgtaaaatt aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta
     2641 gtacacagat cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagcagcaa
     2701 atgaagggga aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg
     2761 aaactgatgg aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa
     2821 aagcagcatt taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa
     2881 cgacttatca gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta
     2941 ataaaggcaa atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata
     3001 cggaattcat ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag
     3061 atccttatgt aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa
     3121 atttgagaag tgttttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca
     3181 acttcattgg ggtctttttt tatttgttac aatcatgagg atataaattt aaaaaagttt
     3241 tttatttcta tcacatgaat caatgcgcca ttaagtagat tctttcatgc tcccctattt
     3301 tttcacttgc cgtatcgctt caaaaacaac tatcataaac tcaaactcga ctgaaaagtt
     3361 atttaaaaaa agactgaaaa tatcccttct ggacttgttc ttcttattct gttatactag
     3421 agaaacacga aagatatttt tatgcgacac ttttcatttg tttttatgaa atttattcaa
     3481 aactggcgta acttcagagt tacaaggatg tattagaggt tagggaatac atcgtttaaa
     3541 tgagaaataa gtaatagtta gtaatagtca cccaaacttt aaattaatga atattgaatc
     3601 gtcagatagc cttctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt
     3661 cagaagtaat cagcgacctt ttttagttca ctaatcgttt ctgcttggaa agtttgcacc
     3721 caatcttctt ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct
     3781 agtagctctt tacctaacgt tatcgcatga gctccagatc gaatggctgt agtgacttgt
     3841 tcacaatttt tgaaactagc tgctaatatt tttgttggac tttgcgtacg ttcgatctca
     3901 gtccgcaacg aatcaatcac tttcatagca tcaatgcctt gattttgcat cctgttataa
     3961 taaggtgcaa tataatctgc tccattatta atagctaaat acccttgtaa ttctgtgtaa
     4021 atagctgtac cggtaatcaa ataaccttct tttttcaact ttttgatcgc ttgaaggcct
     4081 gcttgattca ccggcacctt gataaacgta tcttttccca gttcttctat gataactcga
     4141 gcatcctcta tcatttcttc ttccgttctt cctattgtct gaatatggat aggaatctgt
     4201 ccgattattt ttttgatttc tttcatatgc gagaagaagt ttgacgctcc ttcttttttc
     4261 agaatagtag gatttgttgt tacgcctgtt aaattcaaaa actgttgaca agaataaata
     4321 ctttttgtat ttgctgtatc aagcatgatt tccatatagc tttcccccat tctgatcctc
     4381 ttgatcgatt ttaatgattg tcccctttct taaatacggc atttccttat cttctacata
     4441 cattgttcct ggaagttccg gctcatgaga tccatcaaat tttatcgtga tatgtcctaa
     4501 ttcattcaaa ttagtttgga caacatctcc tactgctgtg attacataca tttgtttatc
     4561 aaagcagagc ttcatatcac aagttatttc ttttcttacc tgattcaatt caatcttata
     4621 acaataatct gttaatgttt ccggtgcttc atctccaaat aaaatcatta ttttttcttt
     4681 cctgaatact tctgcttcta taccaacatc tgttacaacc gtttcaaaga tcgtcatc
//
LOCUS       DANKHK010000136         4637 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000136 DANKHK010000000
VERSION     DANKHK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(52..522)
                     /locus_tag="PW109_002427"
     CDS             complement(52..522)
                     /locus_tag="PW109_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2512202.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="PW109_002428"
     CDS             complement(681..1139)
                     /locus_tag="PW109_002428"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2512203.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="PW109_002429"
     CDS             complement(1310..2455)
                     /locus_tag="PW109_002429"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2512204.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="PW109_002430"
     CDS             complement(2468..3385)
                     /locus_tag="PW109_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2512205.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="PW109_002431"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="PW109_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2512206.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..>4637
                     /locus_tag="PW109_002432"
     CDS             4477..>4637
                     /locus_tag="PW109_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512207.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEREETALIYEEIEMIHKIENIRTI
                     PNYIEAKIF"
BASE COUNT         1453 a          846 c          876 g         1462 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatatggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaattcttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 aaccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga gagaagaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttt
//
LOCUS       DANKHK010000137         4606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000137 DANKHK010000000
VERSION     DANKHK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            32..1093
                     /locus_tag="PW109_002433"
     CDS             32..1093
                     /locus_tag="PW109_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2512208.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGVHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPSIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1096..1992
                     /gene="murQ"
                     /locus_tag="PW109_002434"
     CDS             1096..1992
                     /gene="murQ"
                     /locus_tag="PW109_002434"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2512209.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEVNGFVKGTL"
     gene            2006..3988
                     /locus_tag="PW109_002435"
     CDS             2006..3988
                     /locus_tag="PW109_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2512210.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLPSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLITIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELETETATVNEVPEKAVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIHSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4053..4499)
                     /gene="nrdI"
                     /locus_tag="PW109_002436"
     CDS             complement(4053..4499)
                     /gene="nrdI"
                     /locus_tag="PW109_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2512211.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1488 a          823 c         1047 g         1248 t
ORIGIN      
        1 atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat ttatgaacga
       61 ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg gctttgtcgg
      121 tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa agcgattgat
      181 cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata tttcaggaga
      241 ggcattggaa cgagctggtt tttcattaga agatctcacg cctttgaaag caattggtgt
      301 aacaggttta agaatggatt atcatatttc caatcagcag attgccgact ggtcacatca
      361 attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg aattgaaaga
      421 agcagaagct gatttctcac agattgaagc ttggcataat tattatccac gtcccgaaac
      481 agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct
      541 tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc aaggattgcc
      601 gactttggaa gaacatcgcg gggtccatcc tcttgcaggc gctctagatc tattagcaag
      661 taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc aggagcaatt
      721 tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag atgaagaatt
      781 ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag atgtcatacg
      841 aagcgcagat gcacgttttc gagagattcc gtctatccca gcaagaaata cagctacgcg
      901 tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg gtgagatcca
      961 attaactaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcca aagttatcaa
     1021 agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat ttataagaaa
     1081 agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga acgcagaaac
     1141 caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact aatgaacaaa
     1201 gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga accagtgatt
     1261 gctaacacga ttgaatcctt taaaaaaggt ggacgcctga tttatatggg agccggaaca
     1321 agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg tgtagaaccg
     1381 gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg
     1441 gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt gacaaaaaat
     1501 gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg tggattagat
     1561 tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca agccgaaatc
     1621 agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt gacaggctct
     1681 actcgattga agtcaggaac agcacaaaaa ctgatcttga atatgatttc tactatcagt
     1741 atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc aacaaatgaa
     1801 aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga ttacgaaaca
     1861 gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt catgattttg
     1921 acaaatagtg ataaagagga agctcaagca aaattagctg aagtaaacgg atttgtaaaa
     1981 gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg aagaactggc
     2041 aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg tccactgcat
     2101 gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg aattaaaagc
     2161 gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca tcggacctgg
     2221 gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat taggagaaaa
     2281 acttccttct gtaagtgcag ctacagcaaa ttcatcagcg tctggaaaag accgtgtaaa
     2341 cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa
     2401 atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat ttgtcggtac
     2461 tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg gaacacttga
     2521 tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag
     2581 ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa caccaactct
     2641 tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag caccactgaa
     2701 aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcttag gtgtcatctt
     2761 cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt
     2821 agatatcatc gttacaccaa ctctttcatt gattaccatc ggtctgattg aaattttctt
     2881 catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca actgggtact
     2941 tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc cgatggttat
     3001 gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg aaactggtcg
     3061 tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg cagcattggc
     3121 attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag gagctttacc
     3181 agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac ctctaggacg
     3241 cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg
     3301 aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat tgatctctga
     3361 cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg gcttcgttgc
     3421 tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag agactgaaac
     3481 agccacagta aatgaagtgc ctgaaaaagc agtacctcaa acttcagctg aaagtgtgac
     3541 tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa gtgatccagt
     3601 ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg gacatatcca
     3661 ttctccagtt actggggtag tcagttctgt cttcccaacg aaacacgcta ttgggatcac
     3721 aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg acttaggcgg
     3781 tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata cgcttgtagc
     3841 agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga tcgtcttggt
     3901 aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa
     3961 agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg aaagaagaag
     4021 ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc taaaatctga
     4081 taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa gaaaggaaag
     4141 ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa gtttttattc
     4201 ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag atattcacgc
     4261 atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc gtctaaataa
     4321 gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt ttcatggatt
     4381 tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc agcatattct
     4441 gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa aatgttcatg
     4501 gatcatcccc caattctttc cttttagtat agcatctttt ttaagttttt tggtggtgta
     4561 atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagc
//
LOCUS       DANKHK010000138         4444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000138 DANKHK010000000
VERSION     DANKHK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            354..1517
                     /locus_tag="PW109_002437"
     CDS             354..1517
                     /locus_tag="PW109_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2512212.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            1609..1962
                     /locus_tag="PW109_002438"
                     /pseudo
     CDS             1609..1962
                     /locus_tag="PW109_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(2117..3265)
                     /locus_tag="PW109_002439"
     CDS             complement(2117..3265)
                     /locus_tag="PW109_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2512213.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3282..3686)
                     /gene="tnpA"
                     /locus_tag="PW109_002440"
     CDS             complement(3282..3686)
                     /gene="tnpA"
                     /locus_tag="PW109_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2512214.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            3845..4384
                     /locus_tag="PW109_002441"
                     /pseudo
     CDS             3845..4384
                     /locus_tag="PW109_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
BASE COUNT         1345 a          765 c          839 g         1495 t
ORIGIN      
        1 tttcaaattc cattgcctaa tcattaggaa aagtctatca tgaaatcgta aatagaatat
       61 agtaataacc ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg
      121 aaagtcgcca atgggtaaaa cagaaactgt cggattaagg ctttttccaa tagctaagaa
      181 tgttttcttt acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat
      241 tcttgttcga aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga
      301 gacaaactcg ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa
      361 aaagaaaaaa gatcgattgg tggattttgg gtccttattt gactttgtcc atgatcggtc
      421 ttttagaagt atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa
      481 gtctattatt gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc
      541 gttcagtcaa actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat
      601 cgattttctt tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac
      661 aacgttggat ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt
      721 tgatttttta tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa
      781 aaccattcct gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg
      841 gagcattgat gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta
      901 aaaaagggat ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg
      961 tattatattt aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga
     1021 tcgcgacttt aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt
     1081 cgttttatgc actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa
     1141 aaaaagggta tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc
     1201 ttgggctaat cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct
     1261 tttgtttaag cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa
     1321 ctttgctatt cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga
     1381 ctggtgtgcc attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag
     1441 gaatcggaat cacgttgaat atttcttcaa agatacaggc cgaagagttg ccgctctatc
     1501 gacctgaaaa acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca
     1561 atcttttatt tttcaagcga aaggttcttt tagaaagagg aattcgcaat gaaagtatct
     1621 caaaaaatca tattatctat tttgttactg cttacattag tagttggcta tttttcgatt
     1681 gagtttatcc atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg
     1741 aaaagtgtcc ctaccacact aaatgtggct ttgattggtt cagatgctcg ttcgaaagac
     1801 gaaaacggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
     1861 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
     1921 ataaatgcag cttactctta tgggggagtt gatttagtga actactcacc acttagctaa
     1981 acctgacggt tcttaacgct tgaagtggga gcttcttggg aatagagcat acttgatagc
     2041 gtatttcttt actaacagcg gtgtctctta tttaccaagc tatccccgta gttcctacgg
     2101 ttcttgatga ttggatctaa gccaatccca tagaatgtag tcttagacct tcggtcagaa
     2161 tattgatact agcattgata tcacgatcat gatgagtatg acaaacagga caagtccatt
     2221 ctcgaatttc aagagatttc ttgccatctt tatgtccgca ctctgaacaa atctgactag
     2281 acggaaacca tttatctact ttgatgattt ttcgtccata ccagtcagcc ttgtattgta
     2341 atttagtcac aaactttgac caagaaacat cagaaatact tttagctaat ttatgatttc
     2401 gcaacatacc ttttgtgttt aagtcttcaa tacagataat atcgtgattt ttgatcattt
     2461 ctgtactcaa cttattcaga aaatcagtac gttgattcat tactttttca tgcaatctag
     2521 ctactttgcg tttttgtttt tgataatttt tagcttcaaa tagattggta cctttatttt
     2581 tagccaataa agcacgccta gacaatttgc gttgttcacg ctttagtttc ttttccattt
     2641 tggacgtgaa tttattatta tcaatttttt gtccatcaga aagaatcgca aagtccataa
     2701 tacctaagtc aataccaatt gtggaattag ttttaggtaa ttcgataatt tcttctttac
     2761 acaacaaaga aatatagtat ttaccgctag aatgacgtga tattgtagca gatttgataa
     2821 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
     2881 tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta taactctgaa
     2941 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
     3001 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
     3061 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
     3121 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
     3181 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
     3241 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
     3301 tgattttgga tatacttctt aatcacatca attggtgcgc caccagttgt cagaagacaa
     3361 aagcttttag accagaacat ttctttccaa agaaattgtt tgactcttgg aaagtcacgt
     3421 tttatcaatc ttgagctggc acttttatag gcattgatga attttgtcaa ttctgtttta
     3481 ggttgagctt tgaacataat atgaacgtgg tctttatcat gattccactc cactaaagta
     3541 atgtgatacg attctgaaat tctttcaaaa gtagttttgg cataatcaga tatttcatca
     3601 tcaatcactt gtctacgata tttcgttact aaaacaaggt ggtaataaag aagaaatact
     3661 gaatgattat ttgtatctaa ttccatgtta tatcaactca tttcttgtat agactgattg
     3721 taacatggga caaaataaaa aggcaatacg ccttagttgt cggtcttcga gttaccaacg
     3781 gtaaccctca taccgaacgg cattcatcac ccacctatag aggatgggcg acttctgcct
     3841 aattctgtta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
     3901 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
     3961 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
     4021 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
     4081 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
     4141 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
     4201 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacaata ataaaaagat
     4261 cgatacatta tccgtaccag tagacggcag ttgggacttc aacgacaata cgccttccgg
     4321 aagtgttctg gaattagatt tgaccaaaaa ccaagaagca atcaaaaaat ttctgaataa
     4381 ttaagtaaag aaaataaaag aagttagaaa taacaatcta tgttatgttc tgacttcttt
     4441 tatt
//
LOCUS       DANKHK010000139         4306 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000139 DANKHK010000000
VERSION     DANKHK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            58..1359
                     /locus_tag="PW109_002442"
     CDS             58..1359
                     /locus_tag="PW109_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2512215.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1386..1646
                     /locus_tag="PW109_002443"
     CDS             1386..1646
                     /locus_tag="PW109_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2512216.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2645..3880)
                     /locus_tag="PW109_002444"
     CDS             complement(2645..3880)
                     /locus_tag="PW109_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2512217.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1254 a          689 c          887 g         1476 t
ORIGIN      
        1 ttctttgcgt tgtcatgcaa tgaatggttc atattttatt gaaaaggagt ggttttagtg
       61 gaaaagctga tgaaatgggt cgaagagaaa cttgtcccac caatggcaaa aattggtaca
      121 cagcgtcatt tattagcaat tcgtaatggg gttgtttcaa cattgtcatt gattttgatt
      181 gggacatttt ttatggtctt tatcaattta ccgtttcctg gatggaatga atttattgcc
      241 ccttattcgg caacgattgt tttacccttt aggatgacga tgggattgat ggctatttat
      301 gcaacttttg ttatgggttc ggatctagct aaatcatatg gattagatag tgtgacaggt
      361 ggtatattat ctttaggaac gttctttatg ttacaagttc ctgtaaacgt tttaactcct
      421 gaagaggcac ctcttggctg ggtcttacct atgagttctt tgggagcatc tgggatgttt
      481 gcggggatct tatctatgat ttttgcagtg gaagtatatc gtttctttaa aaatcgaaat
      541 attactatca aaatgccaga acaagtgcca cctgctgttg cccgtagttt tgaggcattg
      601 attccaggtg cagtcatttt aacaattact tggctgattc gaagcgtcgc gggctttgat
      661 gtaaacgctg ccttattatc gttattcgag cctttaacca atattttagg gaataatttg
      721 cttggtgttt tactaccaat gtttttgatc catttgttgt ggtcatttgg tatccatggc
      781 atgagtattg taggggctgt tgttcgtcca atgtggctga tcatgttgga tgagaatgca
      841 aaggcattgg cagatggtac agctgccacc caattaccct atatcgctcc tgaacagttt
      901 tatcaatgga cggtaacaat tggtggtgca ggagcaacga tcgtggtatc tgttttgttt
      961 atattcttct gtaaatcaaa attcttgaaa gaggttggtc gtttttctat tattcctggt
     1021 atcttcaata tcaatgagcc cataattttt ggagctccta tgatgttgaa tccttacatg
     1081 ttcatcccgt tcaacttagt tcctttagtt ttgactatta tcagttattt tgcagtcaaa
     1141 ttagaaatgg tcaatagatt tactgttttg ccaacatgga cattaccagc tccaatcggc
     1201 ggttatcttt cagcaggaaa tgactggcgt gttgttatct tgatcatgat caacacattg
     1261 atagcattta ttatctatta tccatttgta aaagcttatg ataaaaagat gctgtcagat
     1321 gaacaaagtc gtggagaaaa cgaagtccag actgtctaag tttggttttt agaaggtggg
     1381 aaacaatgta ttggctgatt ttattttttg tttttatctt tttattaacg gctagtcacc
     1441 ttatattgaa tatgctagct acatatcata ttcaaatcaa tcgatggatt tgggcactgg
     1501 catcattttt gatcgtgatt cttccaaaga tcattgtacc gcatatgaat gttttgtttt
     1561 catgggggac atatgtactt tgtggaattt ttgctatcaa ttttatgatc gagcagcata
     1621 gatggtttgt cacatcgaag ctttaaatgt tctaaaagtg gttgtaacag aagtggttag
     1681 cttcaagaaa taagaagaaa tttacgaaaa ttgcttctta aatttttgtg aaatgttggc
     1741 ttatttttcg tttggttgaa cttattaccg cagatgcaag cttttgacca ccgtttattc
     1801 ggaatatggg actgtgacaa ggcttttgtc acagcccttt tttacataaa gattattatt
     1861 gttttaactg ttttggcaga aagaaggagg aaaaaatgga aattttatca gcaatggatt
     1921 tagaattagt agatctacta ccaaagtgat caagtcaaat atagatttgg gagcttacaa
     1981 ccatatagta ggggctgcag tatggtgtat gaatggtaat atgtatgatg tgagtgttta
     2041 ttcaaatcat gaaacgtgta tagaaataat tgtgctggga atagcaatca gtcatggtga
     2101 acgtgacttt tagtgtatcg ttgctgacga catagaacat tctgacatga tctactcaat
     2161 atgtggggat tgctggcagt ttatatagga gtatgcatcg gattgcgaga tcattgtctc
     2221 aacagaagag ggaggttgat tatcaaagat aaagtggtaa atcctacatt tgatttagta
     2281 attgttgagg ggactagcgg cacaaatctc tacaaagcga tgcttctaga tctatatgac
     2341 acattttaag aaaatgtatt tgcctaatta ttatggtctt tcttttgaag aaacgttgag
     2401 atgatccaac cagagaaagg tgtcaaagaa tttgactaag aataaatggc agacgctacc
     2461 tatgctcatt tggatagcgt cagatctttt attttaagga tgattttgtg ttttttagac
     2521 accgtaattt aagtaactct aacttttcaa tccactattt actttttttg tattttcact
     2581 gtctgggtct cgaatagcga aattactgat gagtctctat taaaaaagct ttttctactt
     2641 gaaactatat tgggtggttg ctcaggaagg tttcgatttc ttgaattgct tgttgtggac
     2701 catttattgg aaaaacggct tgcagcgtgc gtgcctgctg acgaatcttg ggtgaagaaa
     2761 cttcgcgcag agcaatctgc agttttcttt cagtcactgg ttttgtgagg gcgattgcgt
     2821 ttccgtattt tacacaatag tcaatattac agctttgctc aaattgcatc cccacaccga
     2881 taaacggttt gccggattca caggcagttt ggacggtccc ttccccgccg tggataaaag
     2941 aaaaatcaat gaattcatgc aaaagatgac tagacagata ttctgttatt aaaagatctt
     3001 ctgagtcaga ttgaatgtca tcaagcagat agtcaatagg tgcaataacc gctagatccg
     3061 gttgtttttg taagaagaac aaaattttgc gtaacatacg cggattgcct gaactaccca
     3121 ttgcgaaata gataatacgt tttctttctt gttgctttcg ctgtataaaa gttagcactt
     3181 cttcaggcaa ggaagtactg agcttagcaa aaattggacc aacgattcga taattttccg
     3241 gcagctgttc ttcttccaaa aatagtttag gcgttgtgat gagatttaga tcagcatcca
     3301 tcatttgtaa acttgttttt ggtaaaggaa gcatttcttt ttgtgcagcg tgtttgaagg
     3361 cattggggat ccatcgggta tttactatac gcttttttgt ccagtgccaa aggggattgc
     3421 ttaaagggcc gaagaaacgt aactcgtggg gcatcagcgc atgctctgat tgaaaaaaag
     3481 gttcactcaa agcatagggt ttaacatata ctaaaggaat ctttttattg cgggccgata
     3541 aaaaaattgt cacattcgaa cctgtcacaa tcagtcgagg ctcaaactta gcaatcagac
     3601 tctgttcact agtgatacga gaggcaacca tttctttggt aaaagggttt ttaagtgtac
     3661 gcaactggtc aaattttatg atttgttctt cttgtttttt cgtaaggatc ggcgctagta
     3721 aatgatactc aaagccactt tctgtgatca aaggagcaaa agttttagaa aagccaaaga
     3781 atacacagta ataacggtct ttcatagctt tggcaacctc aatcattcgt gttgtctcag
     3841 ctaaattgaa tactgcagga gcaaaaagaa ttgtcttcat gaaatcctcc ttattggaat
     3901 gagtctatcc cttcgtttct tactagtata ctaaaaaaca cagcgttcga gtagcctatg
     3961 tatattttga atattaaact gtctctcgct gttttcacaa cttctaaatt taaattttgt
     4021 aatcaagttg aaactccaat tttcaaaaaa acacgcaaca attttttgat cgatttatat
     4081 gttgtgccag ataaatacga gaagttgtgt cattaaaaat aaacatgaga acataaaaaa
     4141 ataaaataat tatattaatt gataggtgtg ctgtcgtcat tatacacacg taagaaagaa
     4201 atagttatgt ttaaaaatat tcttagactt tctcgccatg acaattttca ctatgtgtta
     4261 taactgtaaa cgataattat tcagataaca caaaaagaaa tcaagg
//
LOCUS       DANKHK010000140         4289 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000140 DANKHK010000000
VERSION     DANKHK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            430..990
                     /locus_tag="PW109_002445"
     CDS             430..990
                     /locus_tag="PW109_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2512218.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            987..2879
                     /locus_tag="PW109_002446"
     CDS             987..2879
                     /locus_tag="PW109_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2512219.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2886..3263
                     /locus_tag="PW109_002447"
     CDS             2886..3263
                     /locus_tag="PW109_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2512220.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3414..4196
                     /locus_tag="PW109_002448"
     CDS             3414..4196
                     /locus_tag="PW109_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL2512221.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT         1609 a          572 c          853 g         1255 t
ORIGIN      
        1 attaaatact tagataatac tggtaagtct cctaatacca ttaaggcata ctgctatcat
       61 ttaaagttgc tgtacgagtt catggaacag agaggtgtta ttcttaatga tattaacttt
      121 gagttgttag cagacttcgt aggttggttg agatatcctt cagcatcaaa tgtaattgat
      181 cttcagtcaa aaaaagccat aagagaagaa acgacagtga atacaatttt aaatgtagtt
      241 atgagttttc ttgattattt aagtagatta ggagaattta aatcaattga tgtatttaaa
      301 caagcaaagg gaagaaattt caaaggattt ttacatcatg ttaataaggg tagataccaa
      361 aagaatgtct taaagttaag ggttaaaaag aaacagataa gaacattgag atcaaaggaa
      421 gttaagcaaa ttattgatgc ttgtcatacg aaaagagata aattaatttt aatgcttatg
      481 tatgagggtg gtttaagaat cggtgaagtg ttatcgctta ggcttgaaga tattgtcact
      541 tgggacaatc aaatccattt aacacctaga gatgttaatg ttaatgaagc ttatattaaa
      601 ttaaggaagg aaagaacaat acatgtgagt aaagaactta tgtcacttta tacagattac
      661 ttgatatatg agtatagtga ggaattggag catgattatg tttttatttc cttaaaagaa
      721 ggctattttg ggaaaccact aaagtaccaa agtgttcttg atctagttag aagaatagtt
      781 aaaaggactg gaatagaatt tacatcacat atgcttcgcc acactcacgc aacgcagcta
      841 attagggaag gatgggatgt tgcgttcgtt caaaagagat taggtcacgc acatgttcag
      901 acaacgttaa atacctatgt tcatctttca gatcaggata tgaaaaatga gtttaataaa
      961 tacctcgaga gaaaggagca taagaaatga atgcttctag taaaaggaaa attattagtc
     1021 agagtgagat tagcaaaaaa atagctgtaa tgaatgaaga aatgcagggg ttttgggcta
     1081 ataatagttg ggatataaga aaatgtccac atccttctgc catagaatta agtaagaatc
     1141 ctgctttaag gaatcgttgg gttcgttttg aacgtgttaa aaatctgtgg ttaagaacag
     1201 aattgaaata tttttatttt taccatttaa acaatggaat atggaatgca aaaactgtct
     1261 ggattagaaa aggaacagta attaataaaa tgttagattt cttagattta aagtatccta
     1321 gcattacttc aattactgaa gttcctattg aaaaagcaat gacggagtat agaacttatt
     1381 tgacaaaacg gggtgttaga attaccacta ctaattataa gattactgct aatcaagaaa
     1441 aaacacctgt aaaagctaat tcctactatg ttactaatct aaaacaattt atggagtttt
     1501 atgagaactt ttattttgat ggagaggagt gggataaaga cgtttgggat agacgtaact
     1561 tacctttgcc agatgataag gttaacccaa cacaatatga atatacaatt aactttaaag
     1621 ggtttcggaa tacatatttt aaacaacttg taaaaagata ttgtaagttg agattgaacg
     1681 tggatagctt ttcctatgta agtgatattg cccaaagact taaagagttc tttaattttc
     1741 tggacatgaa atttaaacaa gttcagagag tacaccaatt aacgagagtg gaaattgaag
     1801 catatttaag tgaactaaac atgatgggaa taaaacctag tacaataact gggaggatct
     1861 ctatattgga aggactattt agtacccttc ttaggctaga atgggatgat gttccttcca
     1921 aaatattaat ttattctgag gactatccga aaataccaag agcaaaacca cgctttatag
     1981 atgaattcgt cctagagcaa ttgaacagtc atcttgataa attacccgaa tatatagcta
     2041 cgatgactat gattgttcaa gaatgtggaa tgaggataag tgaattgtgc accttgaaaa
     2101 aaggctgtct attagaggac aaagatggag atttcttttt aaagtattat caatggaaaa
     2161 tgaaaaagga gcatatagtt ccaatatcta aagaggtagc tttacttatt aaagttcggg
     2221 aagataaagt ttcagaggaa tttccagata gtgaatacct ctttccaaga aaagatggat
     2281 cgccattaaa acaagaaaca tttagaggtg agttaaataa attagcttat gagcaaaata
     2341 tagtggataa atcaggtgag atttatagat tccatgccca tgcctttcgc catacagtag
     2401 gaacaagaat gattaacaac gggatgcccc agcatattgt gcagaaattt ttggggcatg
     2461 aaagcccaga aatgacaagc agatacgctc atatctttga tgaaactcta aaaaatgaat
     2521 ttactaaatt tcaggaaaaa ctggttacca ataatggaga tgtgcttgat ctagatgaag
     2581 ataatgaagt cgatgatgta gagcttcaat ggttcaagaa aaatataaat gcacaagtgc
     2641 ttccaaatgg ttattgtaga ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat
     2701 gcttagattg cactcacttc tgtaccagta agcaattctt accacagcac gaagaacagt
     2761 tagagcgtac agaagagtta ttagccatag ctaaggataa acaatggcaa agacaagtag
     2821 agactaatag ccgtgttaaa gagcgtttag aacaaatcat tggaagtttg acggggtaat
     2881 tatcaatgga taaacaagtt agaaatacaa cagaaattgt acgtttggcg aagcagaaat
     2941 caaaaaagac aagggaaaaa gtagacaaag cgatttctaa attttcgatt gaaggtaaag
     3001 ttattaattt taattcaata gcaaaggaag ctaatgtttc taaatcatgg ctttataagg
     3061 aacacgatat taggcaaaga atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag
     3121 tctcaaaacc caagaaaagt tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa
     3181 gagtaatgga attagaaaaa gaaaataaaa aattacagaa ccaaattcaa aaattatatg
     3241 gagatctgta taataaagaa taattattaa tctgtagaca aattgtgaaa ggatgtactt
     3301 aaacgctaac ggtcagcttt attgaacagt aatttaagta tatgtccaat ctagggtaag
     3361 taaattgagt atcaatataa actttatatg aacataatca acgaggtgaa atcatgagca
     3421 atttgattaa cggaaaaata ccaaatcaag cgattcaaac attaaaaatc gtaaaagatt
     3481 tatttggaag ttcaatagtt ggagtatatc tatttggttc agcagtaaat ggtggtttac
     3541 gcattaacag cgatgtagat gttctagtcg tcgtgaatca tagtttacct caattaactc
     3601 gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa gattggaaat acggattctg
     3661 ttagaccact tgaagttacg gttataaata ggagtgaagt tgtcccttgg caatatcctc
     3721 caaaaagaga atttatatac ggtgagtggc tcaggggtga atttgagaat ggacaaattc
     3781 aggaaccaag ctatgatcct gatttggcta ttgttttagc acaagcaaga aagaatagta
     3841 tttctctatt tggtcctgat tcttcaagta tacttgtctc cgtacctttg acagatattc
     3901 gaagagcaat taaggattct ttgccagaac taattgaggg gataaaaggt gatgagcgta
     3961 atgtaatttt aaccctagct cgaatgtggc aaacagtgac tactggtgaa attacctcga
     4021 aagatgtcgc tgcagaatgg gctatacctc ttttacctaa agagcatgta actttactgg
     4081 atatagctag aaaaggctat cggggagagt gtgatgataa gtgggaagga ctatattcaa
     4141 aggtgaaagc actcgttaag tatatgaaaa attctataga aacttctctc aattaggcta
     4201 attttattgc aataacaggt gcctactttt aaaacaaccg atttattaat aaatattgaa
     4261 aatttttagg aagaataaag cgttctctt
//
LOCUS       DANKHK010000141         4079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000141 DANKHK010000000
VERSION     DANKHK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            57..1481
                     /locus_tag="PW109_002449"
     CDS             57..1481
                     /locus_tag="PW109_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2512222.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWIFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1662..2435)
                     /locus_tag="PW109_002450"
     CDS             complement(1662..2435)
                     /locus_tag="PW109_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2512223.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2432..3337)
                     /locus_tag="PW109_002451"
     CDS             complement(2432..3337)
                     /locus_tag="PW109_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2512224.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQA
                     AQDFIIISTTNDLAARTILTEAGYTLEDSDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3491..3811)
                     /locus_tag="PW109_002452"
     CDS             complement(3491..3811)
                     /locus_tag="PW109_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2512225.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1231 a          750 c          810 g         1288 t
ORIGIN      
        1 aaaaaaggtt tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg
       61 atacagagca acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt
      121 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc
      181 cagctatttt cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca
      241 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
      301 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
      361 gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg
      421 aaggtgggca aaatttggca tcgatcgtga tgagtacagt cttcttatgg ggatatgcat
      481 ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta
      541 aattagtgcc attattttta tttattgtag tagctatcag cacgttttcg atccaacaat
      601 tcattgataa cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt
      661 ccagtcaagt attgagttgc atcatggtga tgctttggat atttgttggt attgaaggag
      721 cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag
      781 ggatattcgg cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga
      841 cgatgaatca gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga
      901 tggttggtaa atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg
      961 tttggctctc atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac
     1021 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
     1081 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata
     1141 actttgctta cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt
     1201 accaaatgaa actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag
     1261 caagtgcctt tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc
     1321 ttgttctttt cttgccaggc atttattttt atctcaaagc acgtaaggag caaaacgagc
     1381 ctgtacaaca tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga
     1441 ttgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa
     1501 ggagaggttg tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa
     1561 atgttgtcac agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata
     1621 aggctgaacg tctctttcac aacctctttt ttgatgactc atcatacatt ttttcgctta
     1681 aatacgagat atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaacagcatt
     1741 tgattagtag aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc
     1801 agattcaaca tactgatcgg attccatttg atccactccc atttttggat gagtgcgata
     1861 aggataccag atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgca
     1921 ccggaactat tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg
     1981 atataactac ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc
     2041 ttctccgtga aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata
     2101 gctaaaaggt ataaataaac agaataaata actaatgtga tccatttgct tactaatatt
     2161 tctcctcgag aatactttct ataaaggaga tttttcaatg taccatattg tgcttccatc
     2221 gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa
     2281 agctgagcag tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct
     2341 gttcctatga gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc
     2401 aatttaaata attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat
     2461 ccagaatttc caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat
     2521 ggatattttg tgcagacaat aactggatca cggttcctaa taattcttcg ttttctttta
     2581 aaagcttgat tttatcagaa tcttccaaag tatatccagc ttctgtcaag atcgttcgag
     2641 cagctagatc attcgttgta gagataatga tgaaatcttg cgctgcttgg ttcaattcag
     2701 ccattgtaca ttgcttgatg atctttcctt gattgatcag gatcagctca tccaccattt
     2761 tttcaagctc actgagtaag tgactggaga tcaagaaaga ggtgccttga gtagctaagt
     2821 cggtaatgat ttttcgcaca tctcttgttg cttgtggatc caacccgttc atcggttcat
     2881 ctaaaataac gatagaagga tggttcagta aggcaagagc aatccctagc ttttgtttca
     2941 ttcccaacga gtaagatttc gcttttttgt gaatgtactt ttccatcttg agctgattca
     3001 cgatagtatc gatagcagat acatcgtttg tttcagagaa taacttcaga tgatcatagc
     3061 ctgttaagaa tgggtagata cccggatatt cgatcaatgc tcctacttgt tccaaccctt
     3121 tatgactaga gggagaaatc tcattttccc caatacggat tgttccatct gagtaatgga
     3181 tcagtcctaa aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc
     3241 caacgatttt gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa
     3301 agttcttgga taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag
     3361 atagtaaaag catacaccag atgagtggag aagtcgctag gtcattggac ttatttctta
     3421 ttttttcata aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta
     3481 ttccaagtgt ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc
     3541 ccacaaagaa ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga
     3601 ccgaaccaac tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt
     3661 tgattgcctt tgataaaaag taaaaactag caatcattcc aatcactgtg taaaaagaat
     3721 aattgatttt agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa
     3781 ttcctgctat gattaattcc atccatgcca tacatacgtt cctccttcaa tataaaaaat
     3841 acacaaaaag aggagaatca tcccttcaaa gacctaatgg aatgatcatc ctctttttta
     3901 ctacccggtg gcagatcgta tatctttttc tgtagtatag cacacctatc cagatatcgg
     3961 caatctcata ctttttagta aagtgtaaca agcattgacg gatatatcgt tagcagaaaa
     4021 gaagatgaat gctttgcaat ttcatagaaa ggagttgtta tttattaatc atttaaaaa
//
LOCUS       DANKHK010000142         4014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000142 DANKHK010000000
VERSION     DANKHK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(90..632)
                     /locus_tag="PW109_002453"
     CDS             complement(90..632)
                     /locus_tag="PW109_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2512226.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            767..1156
                     /locus_tag="PW109_002454"
     CDS             767..1156
                     /locus_tag="PW109_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512227.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1160..1924
                     /locus_tag="PW109_002455"
     CDS             1160..1924
                     /locus_tag="PW109_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2512228.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2053..3000
                     /locus_tag="PW109_002456"
     CDS             2053..3000
                     /locus_tag="PW109_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2512229.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3130..3870
                     /locus_tag="PW109_002457"
     CDS             3130..3870
                     /locus_tag="PW109_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512230.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1330 a          682 c          811 g         1191 t
ORIGIN      
        1 tgtcgatgtt ttttaatcaa aataataaat aaaaactgtc taacatttaa aatttaattt
       61 agatattaga cagtttttaa gtttgtgagt tattttcctt gtcgttttct cactttttta
      121 gtccagaaac cacctgaaat atattttggc tcataagaaa tgataaaagc acgctcttcg
      181 atttctttga tcaggcgata cagttcacgt tcgctttttc taggagaaag tatttcgaga
      241 actagccgct caccttccag ccccattgca gaagtctgtg tcacaccata accgtgttct
      301 ctcagtatac ccggcaaact tttatcttct gcgctatttg ctggcaaaat cgttgtgacc
      361 atgatgtatc ctaatgccaa tttgtcttca atcaaaattc cgacactgat acctactgcg
      421 taacctaacg catagacaaa taaattcagt ggattatcta aacgatctaa aacaagggat
      481 aagccgacga tataaatcgt gatctcgacc atactgacaa gtggagcaat cagtcgatag
      541 cctttcattg tcaacatgaa acgtaacgtg ttcagagtga tataagcaaa attaatgagg
      601 aagatcatcc ccaacatttt tggatcaatc atttgtaaaa ctcctttcat ttatataaca
      661 acaatatcaa aaaaaatcgt aagtgaagaa aaattgaaac tttatacata caaaaaaatg
      721 ataaaatgat tataatagaa ttataaagat aggttaggtg attaaaatga aaacggtcaa
      781 tcgtgtgttt tgtgccagcc acgtgctaag tctccttctc agtatatact taggtgtcta
      841 ttcgattcaa cagccaattg aggtagagaa cattgttttt cagatgtcgc tgacagatgg
      901 tttaatttta agcgtttttt tctttttgat actgtttatt ggtaatattt tcggtagcgt
      961 tgccagtttt ttgaattttt ctatttatcc actcctttcg ctagcattag gagtggtggg
     1021 actttgttct ctgtgcttat ttcccgagcc attcagtcct gcatttatat tatttggtac
     1081 actgaacctg ttccagacaa ctgttggagc atggttgcta tggcgatcag gaaatttaat
     1141 gaaaataggt gagtaacaat tgaatccaat tatattgaaa cgttctgtcc atcatattcc
     1201 agtgttagaa gttgttctga aagaattaag agaggaacgt attcccgttg tcatctatta
     1261 tcacggatgg cagacaagta aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca
     1321 gggcattcgt gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac agccggtttc
     1381 ttctattcca tcctatacat tttgggatag tatctacgga aatctgtttg aatttgacac
     1441 acttatcaag catttagaaa aaagaggtct tctacatgat cagcttgcag ttggcggggt
     1501 ttcgatgggg ggaatgacaa catgcgcctt gttggctaaa catcctcata tagtagcggg
     1561 cgtgtgtttg atgggttccc ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc
     1621 atcggaatat aattatgtcg taccaaaaga ctattttgat ttgatttcat gggttaagag
     1681 ctatgatttg tcattgcagc cggaaaagtt agctggacgt cctctgttct tttggcatgg
     1741 acaagctgac gaaaaaatcc cttatataca aacggaacga tttgtagaaa aaaatcggca
     1801 taagtcttac ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac atatggtaca
     1861 aataccacta atggaagaag cttccacttt tttagcaaaa aatttgataa aaaagtataa
     1921 ataattgaaa cggaaaatca ctcatgacga tagaggattt tccgtttttt attttgggct
     1981 ataataaact ctgttaggaa aatttggtat aattgatagt aagaatgaca acatgttaga
     2041 aaggaagaga gcatgcccac tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg
     2101 acggtctctc gagtcatcaa tcatccggaa caggtgactg acgaattgaa agagttagtt
     2161 ttctcagcaa tggctgaact ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg
     2221 aatcggtcgc agatcatcaa attatttata ctggaagaaa tcgacacaac tgagccctat
     2281 tacatgaatt tgctgatggg aatcgctaaa cggatcggta aagcgcatta ttctctccaa
     2341 ttagttacaa atgatgcctt cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc
     2401 agaaaaaaag acttcgattg gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa
     2461 aattctgaag gatatgatta tgtagacagc gataatgaat atggtacagc aaaagcaacg
     2521 caatatgcct tagaaagaga ttatcaaacg attatttata tcggtatcga agtggacgag
     2581 ccttttgaaa tttcaaggga aactggttat ttatctgtga tgaaggaagt tgatcttcct
     2641 agtaaaatct tgcggtttcc aaatcattcg acagaagtag aaagatatat gacagaaaac
     2701 tgggcagatt ttacagataa aacttgtttt gtatgtagtt cagatcggct agcactagga
     2761 gcagtaagag ggattataaa tgctggagga cacttgcccg aagaatttgg tgtgattgga
     2821 tttgatgggg tatttttaga ccaaatcagt tcacctaaat tgacaacgat caagcaagat
     2881 attatacgta tgggtgaagt atgtggagaa atgttgttaa aaaaaataga agaaaaaggg
     2941 gcgagtcaag gttatcgtcg tttttttcct gaactagtta tcagagagac aacgaattaa
     3001 gtcttattac tatatatata tttatgaaat tcatcgctat tttcttaaaa taatcagcgc
     3061 attgctactg aaaacaatga atctgtgcta ttattgagaa aagttccatt tttgagaagg
     3121 atggtttgaa tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta cgatgatgag
     3181 ttaggcgtaa aattaaccaa cgacaagaag cgagtattat ttgacggaca agaagatctt
     3241 ttgactgaac agaatcagtc cccgtttgaa cagatgaaag aatttcattt cacgaacacg
     3301 aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt
     3361 aaggaaaatt tggaaaagca taaaggaaaa ttaccggatt ataccggcca ttaccaatca
     3421 gaaatcacat cgacaggacg aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa
     3481 agtgaaacaa ggacagagtc caagcatact acacgaaacg catctgttga cacgaaggtg
     3541 cgggagcgtt cttattttgt tcctaaatat gtacccgcat cgattatccc agaccctaga
     3601 gaaagcaaaa tttctgagga tcatttaatc gaatcgatga aaaaaacgga tgactcttat
     3661 ctgttgttcg atacagaacc gacgccgtat caagaaaaaa gaaatggtga gccgtctgta
     3721 cgaacattca atcgaacaaa aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg
     3781 aaaaaaagcc atggtatctt agaacgctca ttacaaggat tgatcgatga tcaaggaaac
     3841 tctttgggag aaaatagcta ttttaaataa gtgatataca aatttatatt cggctacagt
     3901 tggaaaggaa acgctgcggc gtttccttat tttatactct atccgaaagt ttatatcgta
     3961 tcctcaaatt tttttgagtt taagcgaatt ttttctccca ataagtaaga aaat
//
LOCUS       DANKHK010000143         3524 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000143 DANKHK010000000
VERSION     DANKHK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..415
                     /locus_tag="PW109_002458"
     CDS             <1..415
                     /locus_tag="PW109_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2512231.1"
                     /translation="VERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDC
                     GKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFI
                     SSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLT"
     gene            complement(471..2834)
                     /locus_tag="PW109_002459"
     CDS             complement(471..2834)
                     /locus_tag="PW109_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDL2512232.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            3282..3503
                     /locus_tag="PW109_002460"
     CDS             3282..3503
                     /locus_tag="PW109_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2512233.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          985 a          730 c          633 g         1176 t
ORIGIN      
        1 cgttgagcga ctttcaaaag ccattatcgc cttgaaacca gaaggccgta aggcagttga
       61 tattgaaaat tcgattaatg aatggcttca atccgtaccc aaaaatcttt tcaaatcaat
      121 tacctttgat tgtggaaaag aattctctaa ttggaaaagt attagtaata cgaatgatat
      181 tgatatttat ttcgcagacc caggaacgcc ttcccaacgg ggattaaatg aacattcaaa
      241 cggactcctc cgaaaagatg gactaccaaa agaaatggac ttcaaccaag tcaatcaagg
      301 attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac taaattacca
      361 aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctca cttaatttga
      421 caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt ttattggtca
      481 agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc acgaatatag
      541 ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc ttgacttcct
      601 ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt tactacacgc
      661 atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat taaatgatgg
      721 ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa agcaaataag
      781 atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc acgcggatct
      841 tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat ttttagtttt
      901 gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga ttccgttcct
      961 gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca atcaatgatt
     1021 ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg agcacgcgga
     1081 tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt atccatgact
     1141 gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc cttcttttct
     1201 gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc ttgctggaag
     1261 actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga aaggtcatgt
     1321 gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg caagaatgat
     1381 tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa gaacccttca
     1441 tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag ccattcatca
     1501 ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac ttgattcaat
     1561 cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt tttctcaatg
     1621 atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga gcctggtttt
     1681 tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg atcgattcca
     1741 ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat ttctctgatt
     1801 tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga tttcagccaa
     1861 cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg tacttggtgt
     1921 gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt ccatcctttt
     1981 ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc tgtcttcagg
     2041 cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt ttcagccata
     2101 acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca gccaagtccg
     2161 ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg gttggctatt
     2221 tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg attgataaag
     2281 acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc accaatcacg
     2341 acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga aagagagtat
     2401 ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg agaagcaatg
     2461 cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc ttccgttact
     2521 tctggataga tctctgtgta acttccatct aaataagcat tcgataccat gacttgtccg
     2581 ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat gactcggttc
     2641 aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat tggatgtttt
     2701 ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag ataaagttgc
     2761 ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa gtattcttta
     2821 ctagaataat ccatacataa cactcctttt cattattact cttattctag cgcagatact
     2881 cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat ctagccaaat
     2941 gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac attgatccaa
     3001 aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca tcatgcataa
     3061 tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct ggatcgagca
     3121 attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt caatatgtga
     3181 attggtatcc cagctactat gctaatttct acagggatta tccaaaattt ggatcgtgac
     3241 caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt cttttaactg
     3301 tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt ctagttactt
     3361 ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc ggtatcttga
     3421 tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa ttttttactt
     3481 ttctttttct cttttttggt tagctgacac gctaccagac taaa
//
LOCUS       DANKHK010000144         3511 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000144 DANKHK010000000
VERSION     DANKHK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            415..2340
                     /locus_tag="PW109_002461"
     CDS             415..2340
                     /locus_tag="PW109_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2512234.1"
                     /translation="METVQKQQSQEKHNHNHGHDHDHGKMPVVLYFVGLALAVIALFL
                     NEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIGAS
                     LMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNVDV
                     SELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSINGESIPKEKSKGDDVFGSTINGT
                     GTFTMEVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFMLL
                     APFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYLSQ
                     LADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAILRK
                     FEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKTVV
                     YVAVDEEVIGLIALMDVPSEYAKETIEYFREQGIHTTLITGDSEMTGKAVAKQLGIDE
                     VIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEVSD
                     LVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVIIHE
                     GSTLVVILNGLRLLKAV"
     gene            2749..3363
                     /locus_tag="PW109_002462"
     CDS             2749..3363
                     /locus_tag="PW109_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512235.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATISHRP
                     IFQEALDFVREQDIFIVEAIDRLGRNYDEIIYSVNYLKKKNVRLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADTKDPQ
                     RRLVYKSIVQDLENGVAISKIAKDYNVTRQTIYRIKKEIDQLIV"
BASE COUNT         1206 a          548 c          679 g         1078 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac cttctttatc gattggagaa gttgttttta tatccatagt
      121 gtttttattc ctttcatatg acctttcgtt catatctata ttaccatatg agcgaaacaa
      181 tgctaatctt ttgtttataa aagagaagtg gcgttctctt tatcttgaca aaatgtatat
      241 agcgatataa aatgagtata caaacatatg attagttagt catatgtaaa gtagattcat
      301 tacgatttac ttttggaata gacgaaagga tccagaaata gcggaaattt ttgtgattca
      361 agaagtaaat gatggaaaca aaggacttat aaattatata gggaggcaat agaaatggaa
      421 actgtccaaa aacaacagtc tcaagaaaag cataatcaca accacggtca cgatcatgat
      481 catgggaaaa tgccagtcgt tttgtacttt gttggtttag cattagcagt gattgctcta
      541 tttttgaatg aagattatca gttggtacaa aatgttttgt tctcaatcgc tacaatcagt
      601 gccgggtatc acgttattct tcttgaaggc cttggagaaa caattgagaa ttcaaaagca
      661 caaaaaaagt tcatacctaa ttctcatatt ttaatgggat tagcggcaat tggagcttct
      721 ttgatgggga atttttggga aggaacatta ttgattctta ttttttctgg cgcacatttt
      781 ttggaagatt atgcagaagg aagaagcaaa cgagaaataa cgaaattact cgaaatgaat
      841 ccaacgactg cccggttaat cctacctgat ggcagtacaa aaaatgtaga tgtcagtgaa
      901 ttaaaagtgg gagatcaact tcaagttttg aatggcgatc aagtacctat tgatggcgtc
      961 attttgtctg gatctacatc cattgatgag tcgtctatta atggagaaag tatcccgaaa
     1021 gagaaatcca aaggagacga cgttttcgga agtacaatca atggaacagg tactttcaca
     1081 atggaagtca ctaaagaaaa taaagataca gtattttcaa aaattttaca gttagtaaac
     1141 cagaaccaag acaaccaaac aaaagctgcg agtataatcc aaaaatttga accgaaatac
     1201 gttacagttg ttttaattgc cattccgtta ttcatgttat tggctccttt tctacttgat
     1261 tggacatggt cacaaagtgt ctatagagga ttggtccttt tagttgcagc ttcaccgtgt
     1321 gctttagcag cagctactgt ctcggcaacg ctatcgacga cttctaattt agccaaaaaa
     1381 ggagtccttt caaaaggtag ttcttacctg tctcaattag ccgatattaa agcaattgca
     1441 tttgataaga cgggcacctt gaccaaagga aaacctgaag taaccaatta ttattttgct
     1501 gattccgtga acgaagaaaa catgattgat atcgtagtag ctcttgaaaa agaatccaat
     1561 caccccttag cagatgctat tctaagaaag tttgagcaaa aaaacaaact aactattgaa
     1621 gtagaaaacc aaattggtaa aggattgacg ggagattaca aagggagaaa ttatcgtata
     1681 ggaaaaccga cttcgtttga tgatgtttca gatgaatata gtcgtttaaa taatgaatgg
     1741 gcgtcagaag gtaagacagt cgtatacgta gcagtagatg aagaggttat aggccttatt
     1801 gcccttatgg acgttccgag tgaatatgct aaagaaacca ttgaatattt cagagaacaa
     1861 ggtatacaca ccacattaat tactggtgac tcagaaatga cgggaaaagc ggttgctaaa
     1921 caattgggaa tagatgaagt gattgcaaat gtcatgccgg aagacaaatc cagaataata
     1981 gacgaacaaa aagaaaaata cggcgtgacc gccatggttg gagacggtgt aaatgatgct
     2041 cctgctcttg ttaatgcaga tgtgggaatc gctatgggag atgggactga tgtggcagta
     2101 gaggtatctg atttagtttt aatgcaaaac aatttatcta aattggtaca atctcataat
     2161 atttcttcga aaatgaaccg tgttatttgg cagaatatta ttttttcaat ggccgttgtt
     2221 gcttttttaa ttgtcgttag tttcttaggc ttaacggata ttgcaatcag tgtcattatt
     2281 catgaaggaa gtacattggt tgttattttg aatggacttc gattgctaaa ggcagtctaa
     2341 agaatgattg cacaccttca aattaatcga gtaatagaga taattaagga ttacactaca
     2401 ttttttatct ctattattta tttatattta tctaggtgtt caaagtcaaa atgatatgtc
     2461 aaccatactc ttgtggcgtg acttgaatac aagacacatg aaattgtata aaatcatgta
     2521 tgaagaaccc tatacccata gggttctttt ttatgaccgc cagaaataaa cgattatata
     2581 ccataatcgg ataaaatcat gtatcgaaaa tagaagccta cgttttttct ttaattatgt
     2641 ctaatttggg tgtaccctaa atagacatca gtgtaacatt gaaaaatatg gtttttcaat
     2701 gtatgattag ggtgtgtttt aaatagttcg agaaagcagg taaaattttt gaaaattgca
     2761 tatatccgag tctcgtcgat agatcagaat gaacaacggc aaattgaaga aatgaaaaag
     2821 tttggcgctg aaaaaatttt tatagaaaag caatctggag cgacgattag tcatcggccg
     2881 atttttcagg aagctttaga ttttgtcaga gagcaagata tttttattgt agaagccatt
     2941 gatcgacttg gccgcaatta tgacgagatt atctattcag tgaattattt aaagaagaaa
     3001 aatgtacgac tcattatcac cagtttaccg atcatggctg aagcaatcgg aaatcctctc
     3061 ctggataaat ttatcaaaga cctaatcatt caaattttgg ccatgattgc tgaacaagag
     3121 cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc
     3181 tataaaggtc gtcctaagtt atacagtgca gacacaaaag atcctcaacg tcgccttgtt
     3241 tataagagca tcgtccagga tcttgagaat ggggttgcta tctcaaagat tgcaaaagac
     3301 tacaatgtga ctaggcaaac aatttaccga ataaaaaaag aaatcgacca actaattgta
     3361 tgatttcttt ttcgaatcag gtaattatta attagctttg tcaccaatac agacaatcat
     3421 tttagattca tctagctaag ttgacaatat ttatgttaaa gaaaatttag ttaacataaa
     3481 tattgtcaat taagaaattg ttctgattta a
//
LOCUS       DANKHK010000145         3472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000145 DANKHK010000000
VERSION     DANKHK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..2339)
                     /locus_tag="PW109_002463"
     CDS             complement(<1..2339)
                     /locus_tag="PW109_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512236.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATP"
     gene            complement(3011..3279)
                     /locus_tag="PW109_002464"
                     /pseudo
     CDS             complement(3011..3279)
                     /locus_tag="PW109_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          947 a          776 c          562 g         1187 t
ORIGIN      
        1 ggtgtcgcat ctaattcata ccctattggc gcttgagttt ctaccagctg atatgctcct
       61 ggtgctaaac cgtcaattgc cagttttcca tcttctcctg tagttaaacc ggtttgtagc
      121 gtttctcctt ctcggttctg taactcaaaa acagctcctt gaaggacttc accactgcct
      181 tcagcatgtt taattagttc aacaccaccc gtatcaagga tgttttcaat ggttaacgtc
      241 acttcttcta tggagtattc atttataaaa acatcatgta ccgtcgcatc taaaagatac
      301 ccttccggtg cttctgtttc tcggacttga tagctaccta caggaagcgg ttcacttaaa
      361 aattctccgt tttctgccgt tataccttct tgtatcactt gtgaatcgct tgataaaatt
      421 tcaaattttg ctcctgccag cctttggcca ttctcatcag ttttaattac tttaatacgc
      481 ccatcgattt tccttgttct tacagtactc gtattcaatc gattgtcact attcacaagc
      541 gttgcaaagt ttaaaatttg atcgccgacc gcatacgcct ctacattgac cttaaaagag
      601 agggtaaaac ttgtcggacc accaatcgta ttattcgcac tgaaacgaac tattccatta
      661 ttgacttcgg catcaatgaa agagggaata tctgatagaa atgacacaaa ttccatgcct
      721 tcttccaaaa catcttcaat aacaagcgta ttgcttgttc cagaaaacat cgagacatgc
      781 agagtatacg ttagttcatt ttcattgaga ttgataacat ttttgtccac actttttctt
      841 aaagagattg ccggaagatt actttcaggg attctcaagc ggtagtctcg gctcattacc
      901 tgattattgt aggtcatact cgctcgattc cgagaaaacc catcctcttg tcttccttca
      961 ttgaacaagt cgacatcggt cacttcaaga gcatattcaa taaaatacga ttgaccctct
     1021 gctagattac caaagtccac aacaagtgta ttcgtttcct tattaaaaga tactttgtcc
     1081 acaaattcct cggaaacata atgagttcta acgtttgctt cacctggatt cgttacttct
     1141 gcgatccgaa ccccacctga aataggttct ctatttccag tggaactcca tgtagtcgga
     1201 ttcggtgcca aagttgttcc ttgcggtagt acatcggtaa ccactacacc gtcttttcgt
     1261 gatccttcat aatttacctg caaaataaat ttattactgt tggtcacgtc ttgcccgtca
     1321 atcagcagat tcccaagatt tccttggctg tcgatttctc tagagccata gtataatttc
     1381 gaaaaaatgg gtcttgatga aggaataggc gtaattgaaa attccgtgtt tgctgctcgc
     1441 tcagcataag taagactcac ttgaaagtca ttatttggct cagtaccgcc tgattgaaag
     1501 aaagcaactt caaacgtgat actgaatgta tacgactgag cggcttcatt taatccttca
     1561 taatcaaagg taaatggaaa aacaacgtct gtgccttctt ggtaaaatgc cagtggcttt
     1621 aaaggtgaat catcagcgac ctccactcta atgaactcaa ttaagccaat cgatgctcta
     1681 ggaactcgta cgaccatctc cccatattct tcaagaatat cgccagcgcc tccttgaatg
     1741 tatcccttta acgtttgtgt atgcctgctc ggcaaagtgg ggcgctcagc agagatgccg
     1801 atgtcataat tcaagagctc ctcttgtctt tcaatctgag attcctgcgt tatagatggc
     1861 tcagatgatc tggcttcatt aaaagattca cttaagcttt cttctggtat attaaaagat
     1921 tcacttgtgc tttcttctgg tacattaaaa gactcacttg tgctttcttc tggtacactg
     1981 atttctattg agaccgtatt tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta
     2041 cctgaatgct gggaagaata agtaatcaga cgttcgtaat cattgagttg cttcacttcc
     2101 ccttcaatca aatcctcact cttatcaatg accaatgcat ttagcaggtc tggtgttcct
     2161 ctgactcgca actggaactg ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc
     2221 agcgaacgct ctggctgttc tgctaaagac tcagctagaa ccgttatgtt cccaaattga
     2281 ttgatcacaa taagcataac tagaaacata cttaacagtt tatgaaattt gttcctcact
     2341 attactcccc cttataattt tgactaaaca acaaggtacc cgataatttt ctaaaaagat
     2401 tccccaactc aaaatggctg cgttacctcc ctcgtatttt cggttcaact caagcgttgc
     2461 aatgtaagct gtaaacaaag ctaatgattg ccgattgtct tctgttgatt gattcgatag
     2521 ctttgtatga cttttagttg tccgtgtggc ttaaccataa ttatgatttc acgattcctc
     2581 ctgccagtgc cttgcacaag ctggagcgta accctaatag ttattttgtt aatgtactta
     2641 tatactttta tacttttcca ttagaaacaa tcactaaatc gttgatacca aggtcagact
     2701 ctagaatttc atcagtgagt ccctgtattg gttaaccttc atcaatccca aaaaaatata
     2761 tcaatgctta gcatcaaaat gaatatatag tttagaatac ttgggattca tcgataactg
     2821 atcgcttatt tggccatatt atttggcact tggttcaact aaacgcattt aggatgttcc
     2881 ttcattctcg tcatggatgt tctcaaaccg tcattactaa taaactgtcc accgaatcga
     2941 tggttcattt cttgcatttt ccacgtccaa ttactctttt tgctctttat ttgggcgtag
     3001 ttttactttc ttaacttgta tcgtcttcca tcaactcctt aatcatcttc ttagctttat
     3061 tggctcggtt tccttgtaaa aggtatctac aaatagtgac aatctgtatt aaatcttcat
     3121 cattttctgt aatgtcaata atttctatat tctgtaccat atattgcaaa gggtttcact
     3181 aagttcataa ccgaagcgaa ttaaacaact tttgtataga ataactacct tttctatttt
     3241 tccattggta attctatcta ttaattggtt taatcctttt gttgtagttg attccactac
     3301 caagatgatt ctctcgaatt ttaaagcttg aagatgtatt gctatttttg ctaactggcg
     3361 tatttgcaac tatttttccc ttctattctt ccttcattga ttcccctcta aaattactat
     3421 ccataaaaaa ttgcataaat gacaatagta tttatattag tcatactgct tt
//
LOCUS       DANKHK010000146         3434 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000146 DANKHK010000000
VERSION     DANKHK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            11..1750
                     /gene="yfcC"
                     /locus_tag="PW109_002465"
     CDS             11..1750
                     /gene="yfcC"
                     /locus_tag="PW109_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2512237.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1907..3091
                     /gene="iadA"
                     /locus_tag="PW109_002466"
     CDS             1907..3091
                     /gene="iadA"
                     /locus_tag="PW109_002466"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2512238.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3202..3405
                     /locus_tag="PW109_002467"
     CDS             3202..3405
                     /locus_tag="PW109_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2512239.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1082 a          581 c          810 g          961 t
ORIGIN      
        1 agaaataaat ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt
       61 atcgacaccc catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac
      121 ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg
      181 cgaaacaagt actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga
      241 taagtcgtat gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa
      301 actggatgtt ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac
      361 gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga
      421 aaatatctat gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga
      481 ttttggcggc tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta
      541 tggatctgct gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat
      601 tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact
      661 ggagcgttta gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg
      721 gatggcagaa gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg
      781 atatgactcg atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac
      841 ctcttggatg agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt
      901 gtctggtgcg acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta
      961 tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc
     1021 agatgattat ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct
     1081 tgggcataaa ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt
     1141 gactcaaaaa ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt
     1201 tgctggaatc attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc
     1261 ttctttccaa tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa
     1321 agggattctt cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat
     1381 tttgcatggg atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat
     1441 gtacatcttc caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt
     1501 gactatgcca atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt
     1561 tcttgcttat cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt
     1621 gattggtgtg ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa
     1681 gatgcaagga ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg
     1741 tctgcagtag tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc
     1801 caagaaccgt cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt
     1861 tttaagatcc tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg
     1921 acaaattgaa gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg
     1981 gaagattttg gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga
     2041 actttcaggt gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt
     2101 aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt
     2161 gactacagct ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga
     2221 tatgactgcg ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt
     2281 atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt
     2341 tttgacaatt gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc
     2401 tcagccaagt tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt
     2461 atccggaaaa gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct
     2521 attgacacga atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc
     2581 aaatcgcaca ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat
     2641 tgacttcact gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt
     2701 cagcaaagca ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac
     2761 ctcagatgga caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg
     2821 agtaggaagt gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat
     2881 ccctttggaa atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga
     2941 taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac
     3001 gcttgatatt gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa
     3061 agtatggggg actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa
     3121 acgagcgttc tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac
     3181 aaactaagag gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat
     3241 aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa
     3301 acagaaggga aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc
     3361 gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact
     3421 aattgaatca agca
//
LOCUS       DANKHK010000147         3382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000147 DANKHK010000000
VERSION     DANKHK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            9..1949
                     /locus_tag="PW109_002468"
     CDS             9..1949
                     /locus_tag="PW109_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDL2512240.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(2139..>3382)
                     /locus_tag="PW109_002469"
     CDS             complement(2139..>3382)
                     /locus_tag="PW109_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2512241.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT         1015 a          627 c          737 g         1003 t
ORIGIN      
        1 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
       61 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
      121 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
      181 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
      241 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
      301 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
      361 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
      421 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
      481 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
      541 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
      601 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
      661 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
      721 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
      781 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
      841 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
      901 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
      961 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     1021 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     1081 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     1141 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     1201 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     1261 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     1321 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     1381 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     1441 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     1501 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     1561 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     1621 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     1681 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     1741 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     1801 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     1861 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     1921 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     1981 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     2041 tcagcgtcag caatctttga tcgtaagcgc cccatagaac agtacctgta cgtcaaataa
     2101 cccgacctat agaatagaat cgggaatgtt tggcgttttt agttacgaga aaaatgttgt
     2161 tgtacatcct tgttccaagg caacagttga tctaaagttt ctgctgaaag tacgggaaga
     2221 tttggtatct tatcaaatag aaactctaga tactttacag gattcaatcc gttaagttct
     2281 gctgttttat atacactcag aatgattcct gaagttcgtg ctccttcaag acttgtggaa
     2341 tgcaaccaat ttttccgtcc tatcaccagc tctttgatat ttcgctccgc acgattattt
     2401 gtgagttcta aacgaccatc ttcaaaaata ggttcaaaac cacttcgtag tttacacgca
     2461 tattgaacgg ctgcatccaa cttggatttt gcaacaggat ctatcgttgc catccactca
     2521 taaaaagcaa taaataatgg acgcagttct tgttgccgac agcttaatcg cttctcagga
     2581 gaaaagtctt tccatttctt ttctaggaca aaccatttat ccaattgttt gactgcttga
     2641 gctgcgggaa tatccgtgtt tttctttcct tttggaattg cttcgaaaaa tttccgccga
     2701 acatgcgcga aacaatagac aagtcgtaca ctttccacgg cattataccc gctatagccg
     2761 tcacagtgta aataccctgt aaactccttg agaaaagttt tcggaacatc tccagcacga
     2821 ctttcgtcgt ggtgataata aacgatggga tgctgactgt cttttcctgt acaaaactgc
     2881 cagtaatagg tatctgtttt ggcactttca atcacacgat aacttgtttc atccccgtgt
     2941 agaatctctt cttgattaag atagactctt aatcgttctc ctaaagggtc taacgcgttt
     3001 tgcactgctt taatatgcca attggtgata ttatctctcg aaatatccaa cccatgtgtt
     3061 tgtttccaat agttctcttg ccgataggca ggaactttct gaccaaattt catccggatc
     3121 gtttccgcca ctaaactatt ggaacctaag ctattagaaa tcaaaggttt aggaacgatc
     3181 gcttttttaa aagaagaaag tccttctttt tcgcagcttt tacaacaata ggtatgttga
     3241 taatgggtga gacaatccaa catagcctga tggaaacgaa tttcttctcg tacttttgtc
     3301 cgtccgatat ctttcatctg tgaaccgcaa tgttcacaaa attgttcttc caaaggaagg
     3361 agatgttctt tctctgttat ag
//
LOCUS       DANKHK010000148         3338 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000148 DANKHK010000000
VERSION     DANKHK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            279..1688
                     /locus_tag="PW109_002470"
     CDS             279..1688
                     /locus_tag="PW109_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512242.1"
                     /translation="MNHQNEKNIASNVLLISILGLSVLVGSVTADMVNPVLGVIGKEL
                     GGSEAQVSWVVSGVALVLSIAIPFYGRLSDFLNIKKLFTNGFLILTIGSLICIFAPNL
                     IILVLGRMFQGAGMAAIPVLSIVIISKIYPPGQRGRILGIIAGCIGVGTAGGPIFGGV
                     VGQLLGWQSLFWVTFVLGLIIVLGVQISMPKIESPDNNSHQNFDVLGGLLLGLTVGGF
                     LLGITLSEMYGLISIQTTTSFSISMIALIVLIYRVINVKNPFIPPVILKNRLYVSSIF
                     IVFLSMFAYVSMLVFIPLLVVEVNGLSTGQAGLILLSGGVAVAILSPIVGRLSDKVHP
                     KILLLVGLIIMGLSSLYMSFVAGASPVLLSIGSLGIGIAFAFINSPVNNVAVLALPKE
                     QVGVGTGLFQGAMYLGAGTGASLIGALLSMRHGVKASFNPFYTLTAPHYSDIFLTITC
                     IVLVALIVTLNISSRDLKQ"
     gene            complement(2018..>3338)
                     /locus_tag="PW109_002471"
     CDS             complement(2018..>3338)
                     /locus_tag="PW109_002471"
                     /inference="COORDINATES: protein motif:HMM:NF037897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512243.1"
                     /translation="WEDILKWGTVIVPETSSYISDNPIQELDDMGFNSLPLNDNHIIK
                     ENTTTEKRSKIDNLYHGSKEDFTEFKIPRFHKNGGTLGFGTYLTDSLQRAKSYADNDG
                     YLYTIELNDELQNSTPLNASKVTLTTNQVAKIIEKIAQKQIDEEEYPYILSDWEEPTS
                     ETAMDAGNKKIAYNIAEKIIDDTDDLSIINGINNQLGGNDSGAETLIPVLNEMGIHYA
                     ELDVEYYDNSKEYVVFNPKDIKIVNQKLTKPEQSKKFVSDVDIKSLEEKAIKTIAKSE
                     LIVKANNPVEAMQKLYNNDLKSAIKASFILYDLPEDKIDSSKKNVTWEDLVNIEKSYN
                     VFSYYQNNLYGSELVEPDLNNPYQENTKILDNKATQKQPALSDQYKALHDKLKEPGAN
                     KQQIQEQLNKVKIEISQKTQQQLKDFAKANPDLKQPRVKKEQQALQR"
BASE COUNT          947 a          506 c          567 g         1318 t
ORIGIN      
        1 gataaaaatg atggaaatgg tgtttaccct ttataaaaca attaaagttt cagactatct
       61 caaagttgag ggacaagtat gtactttttt aaacagaata ccaaaatcca gactttttac
      121 tataatcctt acgtaaaaaa gtaaaatatt gatttatgta acaaaagtct gatcttaaga
      181 ttaggttttt tatttggata attgagaggt gaaattgatg gttaaagcag tcgatttagc
      241 cttctaaaat gagcatttaa attttaggag gctgtaaaat gaatcatcaa aatgaaaaaa
      301 atatagcatc aaatgtgttg ttaattagta tcttaggctt gtctgtttta gtgggttcag
      361 ttactgcaga tatggttaat cctgttctcg gtgtgattgg aaaagaatta ggtgggtctg
      421 aggcgcaagt tagttgggtc gtaagtggtg ttgccttagt tctatctata gcaattccct
      481 tttatggacg tttatctgat tttttaaata ttaaaaaact ctttactaac ggatttctaa
      541 tattaactat aggaagttta atatgtatat tcgcgcccaa tttaattatt ttagtattgg
      601 gtagaatgtt tcaaggagct ggcatggcag ccatacctgt gctatctatt gttataatat
      661 ctaaaattta tccgcctgga cagagaggaa gaattttagg tattattgct ggttgtattg
      721 gcgttggcac tgcaggaggc ccaatatttg gcggcgttgt aggacaatta ttaggttggc
      781 aatcattatt ttgggtcact tttgttctgg gtttaattat agttctaggt gttcaaatat
      841 caatgcctaa aatagaatca ccagataata acagtcatca aaattttgat gttttaggtg
      901 gactattatt aggattaacc gtaggtggtt ttttacttgg tattacgctt tcagaaatgt
      961 atggtttgat ttctatacaa acaaccacaa gtttttctat atctatgata gctttaatag
     1021 tgttaatata tcgcgtgatt aatgttaaaa atccttttat ccctccagtt atattgaaga
     1081 accgtttata tgtaagctca attttcattg tatttctttc aatgtttgct tatgtctcta
     1141 tgcttgtttt tattccatta ttagttgttg aggttaatgg gttaagtact ggacaggcag
     1201 gcttaatatt actttctggt ggtgtcgctg ttgcaatcct ttcaccaata gtgggaagat
     1261 tatctgacaa agtgcatcct aaaatactat tattagttgg actaattatt atgggcttat
     1321 cttctttata tatgagcttc gtagcaggcg catcacctgt attattatct atcgggagtt
     1381 tagggatagg tatcgctttt gcatttatta actctccagt gaataatgtt gcagtacttg
     1441 ctttacctaa agaacaagtt ggtgtaggta cggggttgtt tcaaggtgca atgtatcttg
     1501 gggcaggaac aggtgcctca ctaatagggg ctttattgtc aatgagacat ggggttaaag
     1561 catcttttaa tcctttttat actttaactg ccccgcacta ttctgatata tttttaacca
     1621 ttacgtgcat cgtgttagtt gctttaattg ttactttaaa tataagtagt agggatttaa
     1681 agcaataaaa cctttttatt acacagatat acatctgttt ttaaatatta ttcagaaact
     1741 aaattgttaa tagcaagaac aactaaataa ttttaaaaaa cagggcttat taacaagccc
     1801 tgttttgcat tttagccaag aaaataacca gtttctgtac actatctaac tatcactttt
     1861 ttcaattcat aatattgtat agcattccat acgtttcatt ttctcattta ttaagtaaaa
     1921 tcattctaat ttagcaagta aagtcacaac ggtcttagaa tgcgcctctc aaacgtcaaa
     1981 atacaaaaaa gcgcctataa ataggcgctt tttatgatta tcgttgcaaa gcttgctgtt
     2041 ctttttttac tcttggttgc ttcaaatctg ggttagcttt tgcaaagtct tttaattgtt
     2101 gctgggtctt ttgactaatt tcgattttaa ctttgtttag ttgttcctgt atttgttgtt
     2161 tatttgcacc tggctctttt aacttatcat gcaatgcttt gtattgatct gataaggctg
     2221 gttgtttttg tgtggcttta ttatcaagaa tcttagtgtt ttcctgatat ggattgttca
     2281 aatcaggttc tacaagttca ctaccgtata agttgttttg ataataacta aacacattat
     2341 atgatttttc tatgttgact aaatcttccc atgtaacatt ctttttactg ctgtcgattt
     2401 tatcttctgg taaatcatat aatataaagc tggcttttat agcgcttttt aaatcattgt
     2461 tataaagctt ttgcatagct tcaactgggt tgtttgcttt aacaattagt tcagattttg
     2521 cgattgtttt tatagctttt tcttccaagg attttatatc aacatcacta acaaactttt
     2581 tactttgttc tggttttgtt agtttctgat taacgatttt aatatccttt ggattaaata
     2641 caacgtattc tttggaatta tcatagtatt caacatctaa ttctgcatag tgaataccca
     2701 tttcatttaa aacaggaatt agtgtttctg ctccactatc gttaccgcct aattgattgt
     2761 ttatgccatt gataatgctt aaatcatcag tatcatcaat aatcttttca gctatattat
     2821 aagcaatttt tttgttgcct gcgtccattg ctgtttcact agttggttct tcccaatcgg
     2881 acaagatgta ggggtattct tcttcatcaa tctgtttctg tgctatcttt tcaattattt
     2941 tagcgacttg attagttgtt aatgtaactt tgctggcgtt caaaggtgta ctattttgta
     3001 gttcatcatt caattcaata gtatacaaat agccgtcatt atctgcataa gatttagctc
     3061 tttgcaagct atcagttaga taagttccaa agcctaatgt tccaccattt ttatgaaatc
     3121 ttggtatttt aaattcagta aaatcctctt ttgacccatg atataaattg tctattttac
     3181 ttcgcttttc agttgtagta ttttctttaa tgatgtgatt atcatttaaa ggtaagctat
     3241 tgaatcccat atcatctaat tcttgaattg ggttatcgct aatgtagcta cttgtttctg
     3301 gtacaataac tgtcccccat tttaaaatat cttcccat
//
LOCUS       DANKHK010000149         3312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000149 DANKHK010000000
VERSION     DANKHK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(68..1702)
                     /locus_tag="PW109_002472"
     CDS             complement(68..1702)
                     /locus_tag="PW109_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512244.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(1706..3079)
                     /locus_tag="PW109_002473"
     CDS             complement(1706..3079)
                     /locus_tag="PW109_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512245.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
BASE COUNT          872 a          730 c          591 g         1119 t
ORIGIN      
        1 tctctaacga tttcaaatcg ctagagaagc gttagcgccc ctctaacgtt gtttgatata
       61 acctagacta gatcgttaac gtaaagggtg tttttaacac aaaaatagcc gtatcctttg
      121 ttttggatac ggctatttga tattgctcat tcaattgttg taccacggca tgcgtgaatt
      181 tcatcgtaat ttccaacatc tccttatggt taggatcaaa ggttattttc tctataaagg
      241 tacgatacaa ggctttgatt tgcttcttct cactctgggc aagaaaacga tccaaactag
      301 tcaatatccg ttggacatct ttgacttgaa tcggctctcc ttgttgaaag atccctaaga
      361 tttcattttc tcgaatgtgc aactgtcgtc gtttttcagt caattcgtct aagcggtctt
      421 ttaacatcgg aaacaattca ggactatctt ccaaagcagc ttcccatttt tcaattttag
      481 tcttcaattc ttcctttcgt tccagtaaca ccacgagttc ttgttccaat ggttggcttt
      541 gtcgcactat ttcttcattt aacgctgcca ccaatcgggg aagaacttct ggtagctgaa
      601 cgatctcctt taatcggtcg gcgacaaatg tttccgcttg ttcggcacga atcgaattgg
      661 catgacacac acttgctcct ttattccgaa agactgaaca agaataatat cgaatccgtt
      721 tctttgtgcc atcctttagg gtatttgtga cattactcgc tgccatcggt gcgccacatt
      781 ctggacaacg taacaagcct gtcaatacat tttcacctgt atggttccat ttgggttgtt
      841 ttccttctaa agcaaggcgt tcttgtactt tttgataatc ttcttccgaa ataatcggtt
      901 catgggttcc tttcactaaa atcggtctcg gattcttccc cttccgtcgt ttggtttccc
      961 aattgatgta gcgcgcatat tctaaatacc ctgcataaat tttattgcgt aagatatctt
     1021 tgactgccgt ggtagaaaaa gcattctttt tgactgtttg atatccttga cgattcaaaa
     1081 aattggcaat cgctcgatac cctgctccgc ctaaatattt tgcatagatc agacgaacaa
     1141 ttttcgcctc ttctggttca atgaccaatt gtttttgacc attcgtgtca ataccactga
     1201 catagcctaa acgtcggcct cctgcataac gctttttatc tttcactaag ctttgcattg
     1261 acatttgaac attctccgca atttggttgc gttcaaattc accgaaactc ccaaggagtt
     1321 gtaacatcaa tttacctgtc gaagtggtca cgtcaaattg ttccgagatc gaatgtaatc
     1381 ctacgtcata tcgcaataat tcctcgactg tttgtaagac atctaacata ttgcgtccga
     1441 gacgggtcaa tttccaaatc actagtagat caaaacgatg gttccgagca tcgttcatca
     1501 ttcgttgata ggcgaaccga ttttttgtgg atttcccact gattccttca tcggcataaa
     1561 cttctaccac ttcatagccc atccgttcgg caaactgtcg tccattttgt tcttgtgctt
     1621 gaatactatg tccttctaaa gcttgttctt gtgtactcac gcgaatatac aatgcgactc
     1681 gtttggtttg tatcatttcc atagatcatt cgttcctttc taaaatattt tgtgtcagtt
     1741 gtcggaagta aatcccacaa actgttttat cctttgttac ctcaatccga caaacatagt
     1801 cacataataa ttgtcgacta gagaggtctt ttagttgaag ccattctttc acttttgctt
     1861 cacactgttg gagtgaaaga aacgaagaag tagacatcgg cttttgaaga gcttgcagtt
     1921 gttgcttgag ttcttccagt gaacaattgc cctcttcaaa tgccttaatc cattgtgctt
     1981 cttgtttctt ttgttggatt actttgcgtt tttcatcttt tcgcttttcg gctaattgtt
     2041 gcgttaattg ggcaatcaag gcttgataga cgccttcgta ttgaaaaaaa gcctgtatct
     2101 tttccaatac ttctgactca accctttctg cccgaagaaa aattccttgg cagcttcggt
     2161 cggtacaatg ataataatga agcgtcttcg tcttctttgt attccattga tacttcgaac
     2221 tcagatgacg accacagaag ggacaccgaa ttttttgacg taataagtac agatacgtat
     2281 cttttttagc tgtacacctt gattggcgaa tctcttgggc ttgtaaaaag gttgctttcg
     2341 agagaatcgg ttcaaaggtt cctagatact tccccaaaga tcctcctttg atttcgccat
     2401 agtacaatgg attttttagg atactataaa tatgataggg ctgaaaagga cgttcgcgga
     2461 agaaaagctt ttgttgcgtc aatcgttgac aaagcttttt gtagccatat tttttggtca
     2521 aataaagttg aaaaatatag cgaacaatcc ttgcttcgtg tacttcttgt tggattttcc
     2581 cattcactaa acgataccca tatggtaccg cagaagacaa atattgccgt tgttgctttt
     2641 tataggaaag tccggcacga acattctcaa taatgacgtt tcgttgttgt tctgcaaaag
     2701 tgaacaataa ttgcacaaat agtttattcg ttgacgcgtg tgagaaagaa gcaggaagag
     2761 gttcagaaat cgaatacact tgaacctgat gttgttggca caactcaata aattctaagg
     2821 ctattttggc ttgacgtgcc aatcgatcat aacgccaaac atagatcacc ccctcttctt
     2881 ctgacgcaca aacccaagct ctaagctgtc gaaaagctgg acgctccgct ttttcccctg
     2941 attctccttt gtctgaaaat cgttggggaa tttgttcata tttctctaaa acatactctt
     3001 tcactgcatc atattgagcg tcattactaa ttttcgcttc ttcataaaca gacgttcgat
     3061 cataacaagc cacacgcatg cttgattccc ctctctatga atgaactaaa taccattttt
     3121 tcacgactct tctattgaac gaacgttgca caaagtgatt ttctaaatat ccttcgttct
     3181 tcaacgcttg aagagtaccc ccgatagatc cagcagatac ccctcttatt tcgaaatcca
     3241 agtaaattgg ttttgtaaaa aaggcttgat ttgaaaaatg ttcttgaata tacagaagaa
     3301 cctctgcttt ta
//
LOCUS       DANKHK010000150         3309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000150 DANKHK010000000
VERSION     DANKHK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            249..1097
                     /locus_tag="PW109_002474"
     CDS             249..1097
                     /locus_tag="PW109_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2512246.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1111..1434
                     /locus_tag="PW109_002475"
     CDS             1111..1434
                     /locus_tag="PW109_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512247.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            1431..2182
                     /locus_tag="PW109_002476"
                     /pseudo
     CDS             1431..2182
                     /locus_tag="PW109_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            2172..2804
                     /locus_tag="PW109_002477"
                     /pseudo
     CDS             2172..2804
                     /locus_tag="PW109_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2933..>3309)
                     /locus_tag="PW109_002478"
     CDS             complement(2933..>3309)
                     /locus_tag="PW109_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2512248.1"
                     /translation="RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDSELFDYVNWWNHLRLHGTLGYETPVGYR
                     NQRLAQRILDNELGCANASEAV"
BASE COUNT          966 a          641 c          622 g         1080 t
ORIGIN      
        1 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
       61 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
      121 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
      181 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
      241 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
      301 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
      361 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
      421 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttggcag
      481 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggctgaga
      541 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
      601 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
      661 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
      721 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
      781 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
      841 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
      901 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
      961 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     1021 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     1081 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     1141 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     1201 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     1261 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     1321 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     1381 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     1441 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     1501 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     1561 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     1621 tacagagagt tctggaaaaa aacagcagct gaacgcgtac caaagaggtc ttgattttcg
     1681 ggaacatcgc gcttatcgtc caggtgattc tttcaaccga atcgattgga aattgtctgc
     1741 tcatactcaa gagtggttat accgtgaata cgattatcaa gaagaagaat gttctcctct
     1801 tttttgcttt tggggttcgc tcagtccaaa atttgaacct ctgctagact tgtatttttc
     1861 tttatggcat ttgagaaaag aaaagcagcc ggaacttctg atattaggca accgcgcctt
     1921 tcatggcaaa gaccccggtc atacctattt tgcttctttg gaagctggcg atgagaaagc
     1981 ttttttcgca cacttgaaaa aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa
     2041 ttctcctgaa gtctcagaag ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt
     2101 ttatttcgta gaaaaacaac tcatggtcca agaaaaagac aagatatgtt cattgccagg
     2161 aggtgaatgg attgacgatt aaacgggaaa aatggctgcg tggcgtttac tcctttgtga
     2221 tactaagtgt aggtgctccg atatttgtag tagctaatca attgcaaaac agctaccttc
     2281 ttatcctaac tattggtgtc ctatgcctta ttgtctgctg tattcctaaa aaatgggtac
     2341 gtttgctgct ttctttgcca gtaatgatag gttgtttgtt tttatatttt ccttcgaaga
     2401 cattttccat attatggatc ttacagtttt tttcgtcagt cacagaggaa ttcttccatc
     2461 tatcccaagg taaactttat tatttgccag aaaagacagc tttttttctc ataatgttgc
     2521 tgatctattt atttattaca ttgaccgtgg attacgacta ttggtacgca ccctttcttt
     2581 ctttgatggc ttacttcatg atgcttaccg tttttcggaa aaaagattta ctgtacgaaa
     2641 tgattgcggt agtaactgtt ttgttcgtgt atctggcagc aaggcaattt ttctccatcc
     2701 ggttgttgtc gggaaacact cgattccagt cattgactac agttctgctt atttgtttct
     2761 taacttttag cgctatcttg cctttatatc ttccgaaagt acactggaat ggcaacagtt
     2821 tttttgacaa attttataag gtgcagaact tctttccgta tgctattccg attgtccttg
     2881 acaatgagcc tcctcggatg tgacgatctt cggcaggagc taacagttaa agttagactg
     2941 cctcgctagc gttagcacat ccgagctcat tatcaaggat tcgctgcgcc aatctctggt
     3001 tacggtaacc aaccggtgtc tcgtagccaa gtgtaccgtg caaccgaagg tggttccacc
     3061 aattgacata gtcaaataac tccgaatcca attgttgtaa ggtttcaaat gtgtattgat
     3121 agacaaattc tactttcaac gacttataag ttgattcagc tacggcatta tcaaaaggac
     3181 agcctttatg actcaatgat cgattgatgt caaaagttgt taataattca tcaatagctt
     3241 ggttatcaaa ctcttttcca cgatcagtat gaaaaatctc aacctctgtc agaggttgtt
     3301 tgatacggc
//
LOCUS       DANKHK010000151         3202 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000151 DANKHK010000000
VERSION     DANKHK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            109..486
                     /locus_tag="PW109_002479"
     CDS             109..486
                     /locus_tag="PW109_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2512249.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            474..812
                     /locus_tag="PW109_002480"
     CDS             474..812
                     /locus_tag="PW109_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2512250.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            946..1389
                     /locus_tag="PW109_002481"
     CDS             946..1389
                     /locus_tag="PW109_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2512251.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1651..2466
                     /locus_tag="PW109_002482"
     CDS             1651..2466
                     /locus_tag="PW109_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2512252.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2476..3138
                     /locus_tag="PW109_002483"
     CDS             2476..3138
                     /locus_tag="PW109_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2512253.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT         1017 a          479 c          652 g         1054 t
ORIGIN      
        1 cagcttagtt ttaatagaaa tgagtattga cttggagtac acttaaagtt gtagaataaa
       61 taaaatacat aagtaaagtg gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa
      121 caagtcagtg aagaaaaggg catttcggcc gatacattgc gctattatga acgaatcggg
      181 ttgattccac cagttaatcg gacaaaaggc ggaatccgtg attatacaga agaagaccta
      241 aaatgggtcg actttacgat ctgtatgcga aatgccggat tatcgatcga gtctttgatt
      301 gaatatattc gcctatctag cgaaggagaa gcaactgtat ttgagcggcg taagcttttg
      361 attgaagaaa gtgaactttt gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt
      421 ttacaaggaa aaatcaagaa atatgatcgt gtgttatcta gaaataaaat ggaatgtatc
      481 aagtaaaaaa atcaaggagc atcgctatgg agttacgctt actaaaatat ttttggacaa
      541 ttgcagaaga aggaacaatc tctcaagccg cagaagttct acatcttaca caaccaacct
      601 taagccgaca actgaaggag ttagaagagg aactaggaac agaactgttt gtacgcgaaa
      661 accgcaaaat gatcttgaca gaagcaggtt attttctaaa aaagccgagc agaagaaatt
      721 ttagatttaa cgaatcaaac cactcaagaa ttcgagaaca gaaaaaaaca attattcagc
      781 ggccgattta cgatcggctg tgtggaagct gataattcga atacattggc actattacta
      841 gaaaagttga tccgtgatta tcccagcgtt caatttcata tccacagcgg aacaagtgat
      901 gatatcacgg agtgactaga aaaaagcctg ttagatttag cgattttgct agatccagta
      961 gatacagaga aatacgaaac gattttgtta ccaagaaagg aaaaatgggg attgctcatt
     1021 ttagaaaatt ctgttttagc aaaaaaagac gtgataacaa aagaagactt tgcgaatgta
     1081 cctcttttga tctcaaagcg tccagaaata cagcgaatgt ttgctgactg gttaggcata
     1141 aaaaaggagc agttgctgat cacaggaacg tacaatttga ttttcaatgt atttgcgcta
     1201 attgagaatc aggtaggtgc tgctttagtc atagaaggtg ctgtaagtca tctttcaaag
     1261 cgagtgacat tcgttccgct tgacccaatg attgaaacga actgcatgct agtctggaaa
     1321 aagaatcgga ttttatcacc tgtagtcaat gaattgattc accgtttcaa agaagctttt
     1381 gaacagtaat aagtatctct aatcgaagaa atttcaatac aaaagcgaga cgtatgacag
     1441 tcttgctttt gtattttttc atttttaaat caaaaaaaat aaagagattt acataacttg
     1501 ttaaataagc gcttacaaac ataagaagaa cggaaatgaa tcagctataa taaccgttgt
     1561 tctggattgt aatcattaca aagaaatgaa gattataact ttggaggtgt tcgaaatgaa
     1621 agaaaaaaag caaaaattcg ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt
     1681 ctgctgattg tattagcttt attgacgatc attccttttt tatggatggt ttctgcttca
     1741 ttcaaagcaa ataatgaagt gttcacgatt ccgattcaat ggatccctaa aacttggcat
     1801 ttagagaatt atctggttat ttggtctcgt attcctttag tgacattttt taagaataca
     1861 gcttttttaa gcgttgtgat cacgttgatt cagttgttta cttctagttt tgctgcttac
     1921 gggttttcta aactaaattt taaagggcgc gatattttat ttttagcata tatcggcacg
     1981 attgctgtcc cttggcagtc ttatatgatc ccgcagttca ttatgatgag aaaagtgggt
     2041 ttaacatata cgttgtggtc gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc
     2101 ttgttgaagc aatattacag tagtatccca gattctttat gtgagagtgc gcggatcgat
     2161 ggcttatcag agtgggaaat ctatcgcaag atcattttac ctcttacaaa accggcgatt
     2221 gccagtttga cgatcatcac attcgttaat acatggaacg attatatggg gccattcatt
     2281 tatttgtctt ctacggaaaa taaaacaatc cagttagggt tgaaaatgtt tattgggcta
     2341 tttgatgcag agtacgcatt aatcatggct gcctcagttg tttctgttct tcctgttgct
     2401 tttatatttt tagcgatgca aaaatatttt gtagaaggga tcgcttcatc aggaatgaag
     2461 gaataggagg tgattatgaa agagcagcgt tttgacaaaa atgtttatat ggaattcttt
     2521 tattggttgt atcaatcatt ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat
     2581 atagcagctg cgttcctttt gtcgatagat ggaaggaatg tcttttcctt tattttttct
     2641 ttacttttta ttggtccagg ttgcatcgtg atgataggta tgacagaaga attagcaaaa
     2701 gaaaaaagtt tttcaccagt acgtttgttc ataaagacgt ttcgccaatt ttggctaaag
     2761 ggattttttt attggctatt tgcttggata gttagtgtga taatgatttt tgattgtttc
     2821 ttttttatac gtttttctta tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt
     2881 ttatcagttt cctttagcat caactgctgg tattttcagg tgagaaatcc agcgtcgaaa
     2941 ccaaatcaag ttctacggat agcgttctac tatacgttga aaaaatggta tgtgagttta
     3001 ctagattttt tactattgac aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt
     3061 attctactag gtccttctat cgtatttggt ttgatttact tcaacaatcg taaattgatg
     3121 agaactatgg atctgtagac tttataaatt gaaactttca attaagaatg acagtttttt
     3181 atgtaaaaac tgtcattctt tt
//
LOCUS       DANKHK010000152         3122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000152 DANKHK010000000
VERSION     DANKHK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(20..712)
                     /locus_tag="PW109_002484"
     CDS             complement(20..712)
                     /locus_tag="PW109_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2512254.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRSGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            707..973
                     /locus_tag="PW109_002485"
     CDS             707..973
                     /locus_tag="PW109_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2512255.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1149..1718)
                     /locus_tag="PW109_002486"
     CDS             complement(1149..1718)
                     /locus_tag="PW109_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2512256.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1792..2115)
                     /locus_tag="PW109_002487"
     CDS             complement(1792..2115)
                     /locus_tag="PW109_002487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512257.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2256..3080)
                     /locus_tag="PW109_002488"
     CDS             complement(2256..3080)
                     /locus_tag="PW109_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512258.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
BASE COUNT          863 a          629 c          550 g         1080 t
ORIGIN      
        1 aaagaggggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa
       61 aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt
      121 atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca acatttccta
      181 aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt actctagcta
      241 gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa ttagcaatgt
      301 gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca atgtgggcgt
      361 aatttgttcc atctgtcagc gtagaatttg cagcacctga tcgagttgga tcaattactg
      421 gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa tcataggcta
      481 gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta tgatcgcccc
      541 aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa ggagtgtcta
      601 atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg tatactttat
      661 agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt
      721 tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa ggaaaacacc
      781 aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt tagaagatat
      841 caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc atatttctcc
      901 cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc aaataaaaaa
      961 gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact aattttattt
     1021 ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa
     1081 ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca
     1141 tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc catcaattga
     1201 gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg cggttttctt
     1261 cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc cacgactccg
     1321 ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc tagatttttt
     1381 gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag ctcttccatc
     1441 ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt tatttctttt
     1501 ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg caagagcgtt
     1561 tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa tatatatacg
     1621 ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt cacttctgtg
     1681 atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc tttactaatc
     1741 gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt
     1801 tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg aaggatggta
     1861 cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg tgatcactag
     1921 atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc gttttcgatg
     1981 tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt catagcgaaa
     2041 aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt cctggagtgt
     2101 tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt ggatcgctag
     2161 ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt cctgataata
     2221 acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagcc attcttggta
     2281 aagtactttg tcagctttga tttgctttcg ctctcaaatt cgctattcag ccaaaacagc
     2341 gaatcaagct tttcagtcac aatcgtcaat ttccgatagt cttgtgtctc ttctatccgt
     2401 ttccaccgct tttcagtaat ctggttacag ctcaagcgca aaattgctag ataatttttt
     2461 tcttttattg gcaaaatctg ctcctctcta aatagcaatt caggaaacaa ttcaaagaat
     2521 agtgttcgac tgtctcctaa aatctcgatt tctgccggat cctttctttg cttcaataat
     2581 tggatgaaca tctcagaaaa ggactgctct ccatcatctg caaaaaaatg cagaaaatct
     2641 ttcaaaaagc aaaacagcag atatcttact tttctttctg aaccaaagat ctgtgctttt
     2701 ttataagaaa aagaaaggtg gtactcgcca aaccactttc tcaatcgctc ttgcgtagaa
     2761 cgatcgtact tctgctcgta aggttcattc aatagcagat ttttcagtaa acaaaacggg
     2821 ggtgattgga tcagaaaaac aaacaagaga taaggttcgc tatatccttg atcataaact
     2881 aactgaacgg ttttcttatg aagaatcagc tgaatgggac agtcgctttc cgccaaacgt
     2941 ttcagatgcc gatccactgt tttaacatcg attttcagtt cttcagctaa atcagctttt
     3001 cgccagatca aatctttcat tagattgatt agtgaaaata aattctgtgc attttttgat
     3061 aatagttttt gccatctcat cgatcatttc tcacctgatt tctaaacata ttgtttgatc
     3121 aa
//
LOCUS       DANKHK010000153         3116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000153 DANKHK010000000
VERSION     DANKHK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            82..1500
                     /locus_tag="PW109_002489"
     CDS             82..1500
                     /locus_tag="PW109_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512259.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1512..2822
                     /locus_tag="PW109_002490"
     CDS             1512..2822
                     /locus_tag="PW109_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2512260.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT         1033 a          482 c          637 g          964 t
ORIGIN      
        1 gccaacattg tgaatataaa aacaagaaaa aaagagttat aattttgaaa acgtttacta
       61 gaagaggtag gaggaattca aatgctttca caaaatgctg ttacagcaaa tacagtaaaa
      121 aaagaaaaaa aggaatttgg gatttccgat aagataggct acatgttcgg tgatctaggt
      181 aactgtttta ttttaggttt agtcaatagt ttcttgatga tttattatac gaatgttctt
      241 ggcgtttcag gggcaatcgt aggtacgtta ttcttgattt cgagaatttt ggatgcgttt
      301 gctgatgtga tggttggaag attatgtgat gtctcagagt taacagaaac tggccgattt
      361 attccttgga tcagaaaaat gaagtatcca ttttgcttag tgactgtcat tttgtttttg
      421 ccattcgtcc aacatcttcc aatgacggtg aagattgctt atatttttgt ttcatacatt
      481 gtttatggga tttttctctc aacaatcaat atcccgtatg gagcaatggc agcagcaatt
      541 agctcaaatc ctaatgaccg cacctcgttg tccacgttcc gaagcattgg ttctgcgatt
      601 ggcggatcaa ctacaggatt cttcattccg attttaatgt atacccataa caagagtgga
      661 caacaagtcg tttcagggaa tcatttcttt tggatttcaa tcggatgtgc tgcaatagca
      721 tttgtcgcat atatgttaac ttgtaaattg actactgaaa gagtaagagt tgtcaaaaac
      781 gaaaaagtat ctgcttctca gttggtaaaa ggtttgttag ctaatcgagc actgattatt
      841 ttagtcattg ttgacatttt cattgtgatc aatcagattt tagctgggac gaatatgact
      901 tatttattca atgattactt ccataataaa caagcaatgt ctgttgctct tttatttact
      961 tacggaacag tagtagtttt agcaccattt gctacaaagc tgacaaaatt atttggtaaa
     1021 aaggaagcaa gcgtatttgc gttacttgct tcttcatgta tgtacttgct gatgtatttc
     1081 cttcatataa caaatgcatg ggtatatctg attcttttat ttatcgctac tttaggatct
     1141 ggtttattca atttgatggt ctgggcattc attactgacg tgattgatta tcatcaatat
     1201 aagacaggct taagagaaga tggaacagta tatggagtaa attcttttgc gagaaaagta
     1261 ggacaagcat gtgcaggagc ggtaggcgga tttatgctgg cactcattgg ttaccaatcg
     1321 tctacaacag gtggagccat ccaatcagtt ttagtacaag aaaggattta tacgatggca
     1381 aacttgttac cagcattctg cttatttgta tccgcaatca ttttgctagt aggttaccca
     1441 ctgaatcgtt caaaaactgt atttattggt gaagaattga aaaaaatcaa tgaaagatag
     1501 ggagaaacgt attgcggaca aaaaaaggga ttatcaatgc tttgaacttt aaaacaaacg
     1561 acactcaaca agatgataca ataatactgc aaaaagcaat cgatcaaggt gcgaaagaac
     1621 acttacctgt atttattcca aaaggcattt atttagtcgg agcccttttt ttaaaagata
     1681 agagtcatct tatctttgaa gaaggtgcag tcctcaaggg gagaacagat atcgaagcat
     1741 ttccagaaat cgatacacga gtagccggcg tagaaatgaa atggccagca gcgattttga
     1801 atgttctctc ggcgaaagat attcttatag aaggaaaagg aataatcgac ggtcaaggag
     1861 accattggtg ggagctgtat tggggaaaag atcaaaaaag cgggacaaga gctgaatacg
     1921 atcaaaaggg acttcgttgg attgcggatt atgctattaa acgtccacgc gcttgtttac
     1981 tgtatcatgc agaacatgtt gttgttcgtg atttgatttt tcagaaatca ggtttttgga
     2041 acttgcaaat cacttattct aacgatgtat tagtagaaaa agtaatcatc cgcaacaatg
     2101 atggaccaag tacagatggg attgatattg attcttcaac aaatgtgcgt gtctatgaat
     2161 gtgatttggc ctgtggtgat gattgtattg ctattaagtc tggcagagat ggcaatggtg
     2221 caagagtcaa tcgaaaatca tcaagaatcg aggttgctag atgtaaaatc cgttcaggat
     2281 acggtgtaac tattggtagc gaagttagtg ctggagttag tgacgtatat attcacgata
     2341 tcaatttttt tcagtcagac tgtggtattc gaatgaaatc ttcaaaagaa agagggggag
     2401 tgatcgaaaa tatcagggta gaaaatctga atatgattga tgtacaattt cctttttctt
     2461 ggataatgga ctggcataat gaatacaata gaaaaaatag caatgacctt gctcaaatgc
     2521 cagaaagttg gcaagcagtg gctgaagaaa tacctgaatc aaagcaaatg acgaaagtca
     2581 aagacataca catcaaaaac gtacaggcat gcctttctcc aggttactca cttccttccc
     2641 gagcttttga tttagtagca tttcctgaaa agcctataga agatgtctgc ttttctcatt
     2701 gccaaataac tgcaaaagaa ttcgggcgaa tcgaagcagt acaaaactta tgtttcaaaa
     2761 gctgtgattt atctatagaa actggcaaca cagctgcaaa caatactttt gacaatcgtt
     2821 gaaattataa taaggtcaag cttaaataat attttagctt gacctttatt ttttttaatg
     2881 atatattaaa caacgttgtc gcgcttcatt gaaacatttt atgaaaattc caaaaaagag
     2941 ttgacacatg tttttagacg tgataagata actaagtcgt taaaaagaac cacttgcgaa
     3001 gcttgattct atcgaaaaaa aacttcaaaa aagttcttga caaataacaa acgataagtt
     3061 atgataaatg agttgctgaa agatttcgaa aaacgaaatc gaaaaaaact tttaaa
//
LOCUS       DANKHK010000154         3104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000154 DANKHK010000000
VERSION     DANKHK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            82..1377
                     /locus_tag="PW109_002491"
     CDS             82..1377
                     /locus_tag="PW109_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2512261.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1512..2978)
                     /locus_tag="PW109_002492"
     CDS             complement(1512..2978)
                     /locus_tag="PW109_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2512262.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         1050 a          619 c          569 g          866 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa
     1381 aaaatctgag ccagaatgtg gttcattctg actcagattt gattttgcac aactcatcag
     1441 tccttattga caaagagccc aaaaaacagg aactgctgca aataagcaat tcctgttttt
     1501 atgttcattg attaatgaat agagcccatt agttttttga ttggttcttt cacagtaaat
     1561 agcaagatac caccgacgat tgctactgca ccgataatac cgaagtaagc agattctgtt
     1621 cccggtgtgt agaagcgtgc aatttgtgca ttgattgctt gtgaagaagc atcagctaag
     1681 aaccaaatag cgattgtttg tgattcaaat gctcgtggtg ctaattttgt tgtcacagac
     1741 aaaccaactg gtgaaagaca caattcagca ataaccacga tgaagaaact tcccaacaac
     1801 cacaatggac ttacttttcc acttgtacca taaagcatac ccggcaacat caatagcaag
     1861 tacgataatc ctgcaaataa aagtccaaga gagaatttca ctactgtaga tggttgacgt
     1921 tcaccaagtt tcgtccacat cgttacgaat actggtgtca gaataaagat gaaaattgga
     1981 ttcaatgatt gataccagct tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt
     2041 tcactagcaa ataaagctaa gatagatgaa ccttgttctt gtaatgacca gaaaatgatt
     2101 gctgcaagga ataatgggat ataagcccat actcttggtt tttcttcagc agttacatct
     2161 tttgaagtca gcattttgat aaagtaatac aacggtaaga taatccccaa tacactgatt
     2221 gtattgacaa aaaagtcaat tgtcagatga ccagttagtt gtgccccgcc gaatactgca
     2281 gcgataacaa caagtgcgat gatcaagtta cggatgaatc ctttacgttc ttcagttgtc
     2341 aaaggattcg gcggtgttgt acccactccg tctaatgtct tgcgtccttg gatgatgtat
     2401 tgtaataatc caaagaacat cccaatagca gctaaagaga accctaaatg atagttatat
     2461 tcttgaccga ttgtaccaac gattagtggt gctaataatg cccctaagtt gacacccatg
     2521 taaaaaatcg aaaatcctgc atctcttcgc aagtcagtct ttgaataaag atgtccgacc
     2581 atgccagaaa catttggttt caaaaaggcg gttccgataa taatcaatgc catcgaaaca
     2641 aacaatgtac ttacaccaaa tggtaaagcc aagacgatgt ggccaagcat aatgaaaact
     2701 ccaccgaaga atacagtgcg ttttgctcct aacactcggt cagataccca tccgccgacg
     2761 atactagaca tgtagatcaa agatccgtag atcgacatga tagctaatgc tgttgctcgt
     2821 ggtaatccaa gtcccccgtt ggcaactgta tcgtacatat aatagataag taatgcacgc
     2881 ataccgtaat aactaaatcg ttcccacatc tcagtgaaga acaaagttga caatcccttc
     2941 ggctgcccga agaagccctt gtcttgctgt ttttccataa tctatacccc aattctttaa
     3001 attttcagaa aatattatac atctttaccg aaaaatttaa ctaaaaaact tatttaagaa
     3061 atataaacta attttctata aaagcaagta atttttaatg gcaa
//
LOCUS       DANKHK010000155         3047 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000155 DANKHK010000000
VERSION     DANKHK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            93..1757
                     /gene="alsS"
                     /locus_tag="PW109_002493"
     CDS             93..1757
                     /gene="alsS"
                     /locus_tag="PW109_002493"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2512263.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1770..2480
                     /gene="budA"
                     /locus_tag="PW109_002494"
     CDS             1770..2480
                     /gene="budA"
                     /locus_tag="PW109_002494"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2512264.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMGFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2610..>3047
                     /locus_tag="PW109_002495"
     CDS             2610..>3047
                     /locus_tag="PW109_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2512265.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEK"
BASE COUNT         1018 a          510 c          691 g          828 t
ORIGIN      
        1 ttttgaatga aacaggttta gaaactgtat cacttatttg tgattatttc taactaataa
       61 ttaaaaataa agcataaaaa ggacgatgtt caatggccga aaaaatgaaa caaggttcga
      121 aatctgtagt agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg
      181 caaaaatcga tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc
      241 gtcacgaaca aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag
      301 gagtagttat cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag
      361 cgacagcaga aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat
      421 tgaagttgac ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca
      481 gtgcagaaat ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg
      541 ctaagagttc aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac
      601 ctgtccaagg gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag
      661 atgatatccg ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag
      721 gtatgcgagg atcaagtgaa aaagaaacgt tagccatcag gcaattagtt gaaaagacag
      781 cattgcctgt tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc
      841 atttctttgg tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa
      901 gtgatctagt tattgcaatt ggctatgatc caatcgaata tgaagcaaga aactggaatg
      961 cggaaaaaga tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta
     1021 tgcagcctga aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt
     1081 cattagagcc tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcaa
     1141 aattaacgga gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag
     1201 aagttattaa cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag
     1261 gtagtcatta tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat
     1321 tcagtaatgg tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg
     1381 tgcgtccaaa tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc
     1441 aggatttaga aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg
     1501 gacactacaa tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag
     1561 actttggtcc agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag
     1621 ctacaagtgt agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg
     1681 taattattga tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac
     1741 cagatcaatt ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga
     1801 cattaggagc tttgatggcc ggcttgatgg atggaacaga aacgattgct catatattag
     1861 aaaaaggaac tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag
     1921 atggcgtagc ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac
     1981 ttacccccta tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa
     2041 ctgcagatgg agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat
     2101 ttttggcagt gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc
     2161 aagaaaaacc ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa
     2221 gcaatgtcca aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg
     2281 cagcgggatt tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg
     2341 gttttgaagt ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt
     2401 ttccagttga tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag
     2461 aaatccaaca agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg
     2521 tatctcgttt ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct
     2581 ccatagatca aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat
     2641 acgaaatttt attattacag caacaaggat tattagaagc agaagataac tggttagcaa
     2701 atttagcaaa ttcatctgca ttgttgaatg aaactttgcc tgagactgtc tttgcaggat
     2761 attatttatt cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca
     2821 cacgtatcaa gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga
     2881 tcgttgatga tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag
     2941 aaatcgtagt gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg
     3001 gagtaacgaa agggtatgat gaaatcgatc aagcatttct agaaaaa
//
LOCUS       DANKHK010000156         3009 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000156 DANKHK010000000
VERSION     DANKHK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            84..1595
                     /gene="gadC"
                     /locus_tag="PW109_002496"
     CDS             84..1595
                     /gene="gadC"
                     /locus_tag="PW109_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL2512266.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1608..3008
                     /locus_tag="PW109_002497"
     CDS             1608..3008
                     /locus_tag="PW109_002497"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL2512267.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT          917 a          497 c          609 g          986 t
ORIGIN      
        1 gacaaaataa tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt
       61 aaataggaaa gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg
      121 ccctgacagc ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat
      181 tacatttggt tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat
      241 gtgctgcaga aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta
      301 gtcaaacttt aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta
      361 cagtaggttt tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt
      421 ttccagagct taatacagat ccatttatca agtttattgg tgtattagtc attttctggg
      481 tactgacttt ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat
      541 ttgtcattgg aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta
      601 tcggcggtaa tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc
      661 agctttcaac tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt
      721 cagcatcaca tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt
      781 tactagtgat tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta
      841 ttccacaaca tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt
      901 tacattttaa cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg
      961 gcgttatggg tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg
     1021 ctcagcgagg tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc
     1081 cgttgattat gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg
     1141 gaggaggcaa caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg
     1201 taggatattt actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa
     1261 aacgaactta caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt
     1321 ttattttatc agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta
     1381 gtgagcaccg aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac
     1441 cttttatcat ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc
     1501 acgtgaaagc tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg
     1561 ttacaaaaaa tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa
     1621 aagataatca agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg
     1681 ttgacttgcc taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat
     1741 tagtacaaga cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc
     1801 aaacgtatat ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa
     1861 ttgataaatc agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg
     1921 ctgatttatg gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt
     1981 cagaagcctg catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa
     2041 aattaggtct tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag
     2101 tttgttggga aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg
     2161 aaaaacatat gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg
     2221 gtattgtagg tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga
     2281 atgatttagt tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg
     2341 ctgcatcggg tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat
     2401 tgaaaaatgt tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg
     2461 tgggttgggt cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta
     2521 gttacttagg tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac
     2581 taattgggca atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc
     2641 aaagaactca tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg
     2701 aaatcattaa tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca
     2761 atcgcgaatg gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc
     2821 cagcctaccc attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag
     2881 cagactttgg aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag
     2941 agttgaataa agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta
     3001 ctcactaaa
//
LOCUS       DANKHK010000157         2978 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000157 DANKHK010000000
VERSION     DANKHK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            66..620
                     /locus_tag="PW109_002498"
     CDS             66..620
                     /locus_tag="PW109_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2512268.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQS
                     ITKRYIGITEDEIGASLKGFKLGI"
     gene            complement(683..1768)
                     /gene="xerS"
                     /locus_tag="PW109_002499"
     CDS             complement(683..1768)
                     /gene="xerS"
                     /locus_tag="PW109_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2512269.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRHLTAYDLNLYKSHLKRRAKENVKKDTTKAKLEDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDDNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKNTLSKRALIYYHRDVERDLAINALILGSGLRLSE
                     VVNINLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNSL"
     gene            2090..2323
                     /locus_tag="PW109_002500"
     CDS             2090..2323
                     /locus_tag="PW109_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2512270.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQTLSDEIDVAISQALTEYDTTFRELVDK"
     gene            2320..2727
                     /locus_tag="PW109_002501"
     CDS             2320..2727
                     /locus_tag="PW109_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2512271.1"
                     /translation="MSVHYLEAKDVIKMNVIQIKKYSPNEPIGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLANFVFLKINGQLLAIDQKD
                     AVNLVVYIATYDKDFDQLKYEVINTIKTHTKPI"
BASE COUNT         1020 a          417 c          524 g         1017 t
ORIGIN      
        1 tcatgattat ggtaaaatat atatataatc atgaactcag aatattttta agaagggaaa
       61 caatagtgag ctataatgta caaccgttaa ggacaaatga agagatacaa gattttcagt
      121 tttggctaag aagaacaagt aatccagaaa gagatagttt tttatttttg tttggaatca
      181 ataatgggtt acgtatgtca gatattatag gattaaaagt tggagacata cgaggtaaaa
      241 gtaagcctat tattgttgaa cataaaacag gtaagcgaaa accgattttt atagataatt
      301 tgagagaaga gattttatta tatactgaag gtaaagaaga aaatgattgg ttatttccaa
      361 gtcggcaaca aggtcgacat ataacaaggg atcgtgtcta tcagatatat gcagatatag
      421 cagaaaaatt aggacgagat gatataggaa cacatacatt aagaaaaact tttgggtatc
      481 actactacaa gaaaactaga gacattgcta ccttaatgtt tatttttaat catagttctc
      541 aatcgattac taaaagatat attggaatta cagaagatga aatcggtgct tccttaaaag
      601 gatttaaatt aggtatttaa tctttgcatg attagagata aaaaaaacca actacgaaat
      661 ctatttgtag ttggactttt ttttataatg aattcaatgc atcaattgta gccgcattgt
      721 ctatatgggt atataaagtc gttgtattgg tacttgaatg tcccaattgt tgggcagtca
      781 ataccaaaga atttgtctgt tggtagagtc tagtagccaa tgtatgacgc agtttatgcg
      841 gactgacttg gactctgaat cctttcgaat attttgctac catccgttcg attgcaatac
      901 cggatatacg tttggcttct ccttggtaaa ttgctagaaa taatgctttt tcgttttctg
      961 tcactttata tctttctttc cgaatagcta gataattagc taaatattcc attgcgaacg
     1021 ctgctatatt gaccgcatct ctcttatttc cttttctagt tactaccaca ttattcttat
     1081 ccaaagatag atcatctaag ttaatattta caacctcgga gagtctcaat ccagatccta
     1141 aaatcagtgc attgatcgcc aggtctcttt ctacatctcg atggtaatag atcagagctc
     1201 gtttgctcaa agtattttta tattcattgg caatataatc tagataattt tgtgtatctt
     1261 cgtctagaaa taattttttc tcaatggcat ttgcacgagc tgctaaagtt gttttatctg
     1321 tgatagttgc tacctggtcc ataggattat cgtctaagta tggttttcct agaggattat
     1381 tggaactttt cgaaagatat ttaaatagaa ctttcaatgc tgttatattt ctattgatag
     1441 tagaagtgga taggcctaag ttattattat cttcgagctt agcttttgtt gtatcttttt
     1501 ttacattttc ttttgcgcga cgtttcaaat gagatttgta caagtttagg tcataggcag
     1561 ttaaatgtct tatcgaggtg attgaagtat caggaacgat agactcactc agaagccatt
     1621 cataaaagag cttataattt tttagatatt ctaaagtggt tcgaggtgat tgatctttat
     1681 cttcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg attgtatcta
     1741 ttttttgata tatttctgta tttttcatgt cattcatcct ttcaataagt aggagagagg
     1801 agtaccccta caattggact ataccaatta tagggacgtt tgtcaattgg tatgctatat
     1861 tttttttgga aaacacaaat tatataaggt tctatataat catataaaat aaatggtata
     1921 tataaatagg cgaggggata gcagtttgta ttctaaaaat ttttttggtt tttcttaatt
     1981 ctatagaaag aacaatcttt ctatagagtt agacatgaga gggactacga ttgatttaaa
     2041 atcaataagc gtatatactg tatatacaaa caaaaaaggg gagaagacta tggcaatcaa
     2101 agaaagaaaa ttcagagaag tagggaattc agtagtatta actatttcaa aggagtttct
     2161 taaagaaaat gggttaaatc ctggagatac ggttttttta gatgaagaaa aacttcgagc
     2221 agcaattaca aaaaaacaaa ccttatctga tgagatcgat gtagcaatat ctcaagcatt
     2281 aactgaatat gatactactt ttagagagct tgtcgataaa tgagtgttca ttacctagaa
     2341 gccaaagatg ttataaaaat gaatgtgatc caaataaaaa aatactctcc taatgaaccc
     2401 attggtgtaa aggacgcgaa tgctttagaa atgtgtgtag gacaacttcg agcctcagct
     2461 tttggtgaag aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg
     2521 ataaagaaac atccttttca taatgcaaat aaacgtactg ctttcttagc taattttgtc
     2581 tttttgaaaa tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagtg
     2641 gtatatattg ctacatatga taaggatttt gaccaactaa aatatgaagt gatcaacact
     2701 ataaagacac atacgaaacc catttaagga gttgctgtat gtgtctttat agtgttatta
     2761 gctgctatag cgaaataatt gaagtaaatt acaaatagct ataattcaaa tccaactata
     2821 cagaaaacgt aaattttcta tatacttgga tatgctggaa tgtttaagag aataattaca
     2881 tttctagaaa atacaataaa aattacagga taattagcca tatcctacct cctctagcag
     2941 ttgctcaaaa gttagcacta agtaacttct tttttgtt
//
LOCUS       DANKHK010000158         2874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000158 DANKHK010000000
VERSION     DANKHK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            95..1054
                     /locus_tag="PW109_002502"
     CDS             95..1054
                     /locus_tag="PW109_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HDL2512272.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="PW109_002503"
     CDS             complement(1487..2059)
                     /locus_tag="PW109_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2512273.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="PW109_002504"
     CDS             complement(2075..2680)
                     /locus_tag="PW109_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2512274.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          906 a          586 c          477 g          905 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 gttggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat ctat
//
LOCUS       DANKHK010000159         2805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000159 DANKHK010000000
VERSION     DANKHK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            444..1937
                     /locus_tag="PW109_002505"
     CDS             444..1937
                     /locus_tag="PW109_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2512275.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2072..2368
                     /locus_tag="PW109_002506"
     CDS             2072..2368
                     /locus_tag="PW109_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2512276.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(2392..>2805)
                     /locus_tag="PW109_002507"
     CDS             complement(2392..>2805)
                     /locus_tag="PW109_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2512277.1"
                     /translation="RLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITFDCGK
                     EFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESFIQS
                     VASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          952 a          444 c          550 g          859 t
ORIGIN      
        1 tcgaaatata tttgtgcgtt ttgcgttgac aatttagagt gaaaagaggt aaacttaatt
       61 ctatagaaac cacagataat tcactattta caaagtagca aaagcagtgg tactctttaa
      121 acagaaaaat tacataaaaa aaagagacgg aaataattga ttttagcaga atcaattatt
      181 tctgaatcac atggttagga catgtgttca cgctcgtctc aagaatatgt aagtagctac
      241 caactactta tattaaaagt tgatcggata ccttttccga ttgcctttat tctaccaaaa
      301 aaaggcgagc aactcaacag aagaacgttt atttagtgta cttaaattaa atattcttaa
      361 acactgaaga gagaacacat actatggctg agattaaagc taaactgtgt tctctttttg
      421 tttgagaaag gacgttacaa tcgatgttga gcgaaattta ttcagctatt cttaaaaacg
      481 gcttacgtac atacaaatat cgaaacagca ccatgaaacc tgcaggtcat attgattcca
      541 gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta
      601 tggtcattac ttcggaagaa gctattttag agaacgaaaa gaaactaacg cattggacac
      661 ctaataccta tcgctttggt acttatgcgg atgattatcg aacagtggtt aaaggtcatt
      721 ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga tatcgattca aaagagaacc
      781 atcaaggcga aattatttta gcctgtttag atcaagtggg ctacatgcca acgctaattt
      841 tagaatcaga tcatggttat caagtttatt ttgtactaaa gacaccaggc tatgtcacaa
      901 agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac
      961 agttggctga aaaattgtcc ggagtggatc taggatgtaa ccacttcgga attgctcgat
     1021 tcccacaaga aaataatatt gtttttaaag aaatgaacta tcagtattct tttgctgact
     1081 ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa
     1141 aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata
     1201 tgttacttca taaagcaaac atcgtcggcg gagaaggacg tcttggtcga aataatgtga
     1261 ttttcacgct ttctttagct tattactctt caggttatgc attggatact tgcgaataca
     1321 atatgttcac attcaacgaa cggttacagg aaccgctatc tgaagcagaa ttagtaaaga
     1381 ttgtacgtag tgcttattct ggtaattatc aagcagcaag cagagaattc attattgaat
     1441 tgtgccagca atgggtttca ccaaccttaa aaaataatga tctgttcatt caacgacgtg
     1501 gctggtggaa atttaaaaag ccacgagatc aaagaaaata tagtcataaa aatgaatgga
     1561 aagaagatct attggcttat ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc
     1621 tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa gcgaacactt gataagatct
     1681 taaatgagtt acgagaggag aataaagtct tctacaaggt aacacgagga agaaatggcg
     1741 gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa
     1801 agagactaga ttattcaaca aggatcacag aggtatttgg actcaacgag agcttagtag
     1861 aagagacatt tagacaatta tcaaccccct tgaaccctct taaagaagtg aatctttttg
     1921 aagtagatac aggttgaaaa ataaaaggtg caaattgcct tatacaatat atctatgata
     1981 cttctgtagg tgttgctccc tttgcctagc tacgtgtagg tggtatactt ataactgcgt
     2041 atgcagtctt atgtatcatg gaggaagttg tatgtcgtta aaaaattatg tctattcaca
     2101 tcctgttaat gtgtttatca ttggtcgttt gggattatct gtggaagtgt tctgtgagct
     2161 ttatggtttt aagcagggga ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc
     2221 tttgccgatt gagtttcttc atgggttgtc cttagcttct ggggagacta tggatcaggt
     2281 atatgattgt ttgtgtgtgt tagagcagga gtatattgag tataaaatag ctaacgagtt
     2341 aagaaaaagg aagaagtata tccagtagta gtttttattt actcttaatt gtcaaattaa
     2401 gttagacaaa tcatcaatac ttacgtaact caaaaatact tccaaaggtg ttcgataatt
     2461 cagtgatttt ctaggaatat tatttcgttt cgatgcgaca gattgaataa aagattcatc
     2521 tactgaattg aaatccatag atttcaataa accatctcta cgtaacaatc cgttagaatt
     2581 ctcgtttagg cctctttgag acggcgttcc tggatcagcg aaataaatgg caatatcatt
     2641 ggcattactg atctgtttcc aatttgaaaa ttcctttcca caatcaaaag tgatggattt
     2701 gaatagattt ttcggtacag attcaaacca atgatttaat ttgttttcaa tatcaatcgc
     2761 ttgtctacca cacggtttca atgtgatgat aacttttgat aatcg
//
LOCUS       DANKHK010000160         2682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000160 DANKHK010000000
VERSION     DANKHK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..120
                     /locus_tag="PW109_002508"
     CDS             <1..120
                     /locus_tag="PW109_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2512278.1"
                     /translation="PTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            1079..>2682
                     /gene="poxtA"
                     /locus_tag="PW109_002509"
     CDS             1079..>2682
                     /gene="poxtA"
                     /locus_tag="PW109_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein PoxtA"
                     /protein_id="HDL2512279.1"
                     /translation="MKGKNMNLAFGLEEIYEDAEFQIGDLDKVGIVGVNGAGKTTLFR
                     LLLGELELDNGSLTSGNARIGYLPQEIVLEDEDITVWDFLFEGRPIKKYEQELEEIYK
                     KLETAVNAEQEALLARMGTLQERLEYFDFYEAETILLEFADKMSIDAELYHRPMRELS
                     GGQKSKMAFARLLYSKPEILLLDEPTNHLDVSTKDFVIKYLKNYRGSVLIISHDIDFL
                     NRIINKIMYINKATHKISVYDGDYYIYKKKYAEEQRIREMAIVQQEKEIKELSDFVQK
                     AKQASQTNHHLKRMGQERALRLDKKRGELQKRNRLYKRVKMDIRPKREGAQVPLEVEN
                     ITFHYSGYPTLYQNLSFQINGRERFLVVGENGVGKSTLLKLMMGILSPDEGCIRFNQK
                     TDIAYYAQELEQLDENKTVIDNVESEGYTPWQIRAVLSNFLFYDDDVNKKVSVLSPGE
                     KARVALCKILLQKANLLILDEPTNHLDPETQKIIGGNFNLFEGTIIAVSHNPSFVEQI
                     GISRMLILPSGRIEPYSRELLEYYYE"
BASE COUNT          852 a          476 c          587 g          767 t
ORIGIN      
        1 cccacgatta aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc
       61 actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagcttaa
      121 atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccacatga
      181 caaatgcaag ctttggaatt tacattattc attatcctgt tgttgtgtgg gtatgctact
      241 tgctttacag ttacctcaac ttaccgatga tatttatcta tattcttgct ttagggcttg
      301 aactgatact gacaccgtta atttatgagc tgtttaaaag gattcctgta gttcgctttt
      361 tagtattggg gataaaaaaa tgagcatttc ttactatcct attaaacgag tatagctatc
      421 ctatcggcag tagcgttgtc tatggttatg gcttgcctgc atattaaaaa agggcaaaag
      481 aaaaacagag gattttaccc ctctgtttcc tcttttgcgt tacatattca gtttacagtt
      541 gtacaatact tggtctataa acaaactgta aagtgagcag cttagatgag ctattgacat
      601 atgcactcaa gctctctata attaaaatat aaaataagag agggtgagca cagtgttatt
      661 cgggaagttt tgcattgatt ttggctctac acaacaatat aagacagagc attgtgtgga
      721 gcccaatcag ataaaatgtt tatttgagag gcagggctat ttaaatcaga aatttgtgat
      781 ttaagagcct tatttgtgtt gcctttcaat aagcaaactc aatgattgga tatgtggccg
      841 tagacaatgt tttgtctacg gccttttgct gtctatattt catctatgtt attggcatag
      901 tgtctgaaaa agcagagggc tacgtctatg ggacgtattg ctacctctgc ttttattttt
      961 taaatgatga tatcagagaa gaaaatatat ccccgcacag gagataatca agcaaggaaa
     1021 actgtaagcc ataaaatgat ggcttgcagt atgtatgacg atggaggaat gatacaagat
     1081 gaaaggtaaa aatatgaatt tagcctttgg gttggaagaa atttatgagg atgctgagtt
     1141 tcaaatcgga gatttggata aggtcggtat tgtcggcgtg aacggagccg gaaagaccac
     1201 cttgttccgc ctgctgttgg gagaacttga acttgataat ggttcactga ccagtggaaa
     1261 tgcccgtatt ggttatctcc cacaggaaat tgtcttggaa gatgaggata ttaccgtttg
     1321 ggatttcctt tttgagggac gtccgattaa aaagtatgag caggaattgg aagaaatcta
     1381 taaaaagctt gaaaccgcag tcaatgcaga gcaggaagca ctgcttgccc gaatgggaac
     1441 attgcaagaa cgcttggagt atttcgactt ctatgaggca gaaacaattc tgttggagtt
     1501 tgcagataaa atgagcattg atgcagaatt atatcatcgt ccgatgagag agctttcagg
     1561 cggacaaaaa tccaaaatgg catttgccag actactatat tcaaaaccgg aaattctatt
     1621 gttggatgag cctaccaacc atttagatgt cagcacaaag gattttgtta taaaatactt
     1681 aaagaattat aggggttcgg tactgattat cagccatgat attgattttc taaatcggat
     1741 tatcaacaaa attatgtaca tcaacaaagc tacccataaa atatctgttt atgatggaga
     1801 ctactacatc tacaagaaaa agtatgcaga ggaacagcgg attcgtgaaa tggcgattgt
     1861 acagcaggaa aaagaaataa aggagctttc cgattttgta caaaaagcaa aacaagccag
     1921 tcagaccaat catcacctca aacgaatggg tcaagagcga gccttgcggc ttgataaaaa
     1981 gcgtggagag ctgcaaaaga gaaatcgact gtacaagcgt gtgaagatgg atattcgccc
     2041 caagcgtgaa ggggcacaag ttcccttaga ggtggaaaat atcaccttcc actattcggg
     2101 gtatcccacc ctttatcaga acctttcctt tcagattaac ggaagagaac gatttcttgt
     2161 ggtgggtgaa aacggtgtcg gtaaatccac cttattgaaa ttgatgatgg gtattctcag
     2221 tccagatgaa ggatgcattc gctttaacca gaaaactgat attgcatatt atgcacagga
     2281 actcgaacag cttgatgaaa acaaaacggt cattgacaat gtggagtctg aaggatatac
     2341 accgtggcaa atcagagccg tactgagcaa cttcctgttt tatgatgacg atgtaaacaa
     2401 gaaagtatct gtgctgtccc ctggagaaaa agcaagggtt gccctttgca aaatcctatt
     2461 acagaaagcc aatcttttga tactggacga gccgaccaac caccttgacc cagaaacgca
     2521 gaaaatcatt ggcggcaact tcaatttgtt tgagggaacc attattgccg ttagccataa
     2581 cccatccttt gtggaacaaa tcggaattag ccgtatgctt attttgccca gcggtcgaat
     2641 tgaaccctat tcccgtgagc tgcttgagta ttattatgaa at
//
LOCUS       DANKHK010000161         2667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000161 DANKHK010000000
VERSION     DANKHK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            69..536
                     /locus_tag="PW109_002510"
     CDS             69..536
                     /locus_tag="PW109_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512280.1"
                     /translation="MDKEAYERKEKDILRVPYTERYQALKKEKEQLKEIEIPIPECYR
                     HKLKKRMQPNQCFVESIKFARDCREAIYCIGQFQRSDFLHAWIEFEDQDYCFDGTLQA
                     FYPKEKYYKYRGVKKLYMRTAAEITQLSNKHEFYGIYPEDWQKVKSLLVSSSS"
     gene            779..2104
                     /locus_tag="PW109_002511"
     CDS             779..2104
                     /locus_tag="PW109_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2512281.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLATELVVMSRA
                     DNTGSGLILASSPTAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQESIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMTIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGVGGLKND"
     gene            2097..2447
                     /locus_tag="PW109_002512"
     CDS             2097..2447
                     /locus_tag="PW109_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512282.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMATE
                     EISKILKTALIKNRSVCIQKEERNVEGYYPEDIVGKILGGDELGIYVGDNKVHYDEIR
                     HIEFYQEHKWSHLE"
BASE COUNT          988 a          413 c          545 g          721 t
ORIGIN      
        1 agttaaaatt tcaaatacac tataaagcat attgtcagta aataagcttt gtagacaagg
       61 agatcacaat ggataaagaa gcatatgaaa ggaaagaaaa ggacatttta agagtgcctt
      121 atactgaacg ttatcaagca ttaaagaaag aaaaagaaca gctgaaagag attgagattc
      181 ctattcccga atgttatcgg cacaagctaa agaaaagaat gcaaccaaac cagtgcttcg
      241 tggaaagtat aaaatttgca cgagactgca gagaagctat ttattgtatt ggccaatttc
      301 aaagaagtga ttttttgcac gcttggattg agttcgaaga ccaagactat tgtttcgatg
      361 gaacgcttca agcattttac ccaaaagaaa aatactataa gtatcgaggg gtaaaaaagt
      421 tatatatgag aacagctgca gaaataacgc aattatctaa caaacatgaa ttttacggaa
      481 tataccctga agactggcaa aaagtaaaaa gtctgcttgt tagtagttcg tcatgaaagt
      541 tataaaaccc gaacaacctt taaaaggaag ttcggttttt tttatagtga atcaagaagc
      601 tttcctctgt atttagttga tatatgaaga tcctaacgca tttaaaatct aataagtggt
      661 cagttatccg tttaaactgc ttgtaataac aatttgaatc ttttttgtcc atttaaacat
      721 tccttcttta caaaagggaa catatgtttg tataattgta acgaatggag tgttatttat
      781 gatggattat tcaaaagagc cagtaaatga ttactttttg atcgatatga aaagctttta
      841 cgcaagtgtc gaatgtatag aaaggaattt agatccatta gcgactgaat tagtcgtcat
      901 gagccgagcg gataatacag gttcagggtt gattttagct tcttcaccaa ctgcaaagaa
      961 aaaatatggt atttctaatg tcagtaggcc aagagatcta cctcagccat ttcctaaaac
     1021 gttacatgtc gttccgccac gtatgaatct ctatattaag cgaaatatgc aggtaaacaa
     1081 tattttcaga aggtatgtag cagacgaaga tctattgatc tattcgatcg atgaatcaat
     1141 cttaaaagtg accagatcac taaacctttt tacgactgaa ggaacacgaa gtcaacggag
     1201 aaagaagttg gctcaaatga ttcaggagag cattaaagag gagctaggat tgattgctac
     1261 agtaggtgtc ggagataatc cccttttggc taaactagcc ttagacaacg aagcaaagca
     1321 taatgaagga tttgttgcgg aatggacata cgaaaacgtt cctgaaaaag tatggaatat
     1381 tcctgaaatg acggactttt ggggcattgg atcgcgaatg aaaaaaaggc tcaatcagat
     1441 gggaatcatg actattaaag atctggccaa ctggaatccg tacactatta agagccgttt
     1501 aggtgtgatc ggtttgcagc tgtacttcca tgctaacggg atcgatcgaa cagatatagc
     1561 gataccacca gaaccaacca aagagaaatc ctatggtaat tcacaagttt tgccacgaga
     1621 ctatactagg agaaatgaaa tcgaactagt agtaaaagaa atggcggaac aagtagccat
     1681 tcgtatcaga cagcataatt gtaaaacagg atgtgtccat ttaaacattg gtacatcgat
     1741 cttagaaacg agaccagggt tctcacacca gatgaaaatc cctatcacag acaatacaaa
     1801 ggaattgcag aattattgtc ttttcttgtt tgataagtat tatgaaggac aagaagtccg
     1861 ccacgtcgga atcacttaca gcaagctagt ttatactgat tcgcttcagc tggatttgtt
     1921 tagtgatcca caaaaacaga ttgatgacga aaaactagac aaaataatag acaaaattag
     1981 gcagaagtat ggttttacgt caattgtaca tgcttcttcg atgttagaag gcgctagaag
     2041 cattactaga agcacacttg ttggtggaca tgccggcggt gtaggagggc ttaaaaatga
     2101 ttgattataa tgatcgtgga cgtttaaagt gggcagggtt ctatttatca gatcacacgg
     2161 aagaaattga tgcggacaat gaacagcgag ccaatcaaaa cttagcaaaa gaacaaatgg
     2221 cgactgaaga gatttcaaaa atattgaaga cggcgctaat aaaaaataga tctgtctgta
     2281 tccaaaaaga agaacgaaac gttgaggggt attatccaga ggacattgta gggaaaatct
     2341 taggcggtga tgaacttggt atttacgtgg gtgataataa agttcactac gatgaaatac
     2401 gtcatattga attttaccag gaacataaat ggagtcactt agaatgaaca caactataaa
     2461 tatatgttga gagtataaaa tattttgtgt aaatagaaaa aagaacgaag cttacaataa
     2521 acattatgat attaattgag tgtattgtgc ttaaaagcca ctgtgaaata agccaacata
     2581 cactaaaggc taaaaaaaac atactacatg aggtaaaata caaaaaaaac acccttatgt
     2641 tataaaaaga attgcaagat ttattgt
//
LOCUS       DANKHK010000162         2530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000162 DANKHK010000000
VERSION     DANKHK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(110..688)
                     /locus_tag="PW109_002513"
     CDS             complement(110..688)
                     /locus_tag="PW109_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2512283.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(708..1376)
                     /locus_tag="PW109_002514"
     CDS             complement(708..1376)
                     /locus_tag="PW109_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2512284.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1391..2461)
                     /locus_tag="PW109_002515"
     CDS             complement(1391..2461)
                     /locus_tag="PW109_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2512285.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          731 a          552 c          386 g          861 t
ORIGIN      
        1 taacaaattt gtatttatga aaaactacct atataaaaaa aacaagatat aacgatcttc
       61 gtcatatctt gcttcttcac ctaactattt ttctccctat ataacagctt tacgcttctc
      121 ctaatacacc attcaaatat aaatcgatca attgcgccac atttagcggt tcttccacag
      181 gtcttttcga ccgagtgatg taaggtgcta acactaagaa aaaatgcctt gctgcgattt
      241 cttttgaaat cccatttctt agcacagatt ggtttgctga aaagatttct tcaaaaggtt
      301 cacgataatt tttcttccat agaaagaaaa gatgatctct tgtttccttt gaaagattgc
      361 tttcaagatc gtgaagcatc agaaacaaat cgaccagatg tgtctcaatc aaaaactgcg
      421 ccatttttgt caaggttact tgaaatcccg tttcgcttgt ttgaacgatt tctctcaaag
      481 cttttccaac atcttttaag tggttttcta aaactgccgt atatagtttt tctttatcac
      541 cgaaataatg atagagattg ggttgggtga tcccaacttt ttgtgcgatc tctcttgttg
      601 aagtgccgag atatcccttg gacataaaca agtcagtcgc ttctttcaaa attttttgct
      661 gtgtatcttt gttgtatcga tggaccattt ttccacctgc tttttggtta ttctttttct
      721 ttcaatacac catcttccat cataaatatt ctatcgcatt tgtcaatcag ccgtgtatcg
      781 tgagtaacca tgatcgttgc tttatttttg tcttttgatt cttttgccaa gatatcgact
      841 acttctcgtg ccttttctga atccaaacta gcagttggtt catctgctag aataatggac
      901 ggatcattgt acaatgcacg agcgattgcg accctttgcc gttctccacc agatagttct
      961 tctggatact tgttgatcaa cttatccacg ccaagctgct caaataattc ttttgcttct
     1021 ttttgacgat gttcatgttt gatcttatct actaataata actgcttttt tactgtcaga
     1081 aaaggaacca gattggaagc ttgcaaaata aagccaattt ctttaaaacg tagtttaacg
     1141 cgttcacttt ctttttgatt actgaattct tgatcattga taaatacaga gccactgcta
     1201 ggcgtctgca atccgcctgc caaagtcaaa aaagtacttt tacctgaacc agaaggaccg
     1261 atgattgcaa caaattcccc tttatcgata ctcatggttg tttctttcaa tgctttaact
     1321 acttggtcgc catcttggaa acttttttct acttctttaa attctattgc tttcatgctc
     1381 ttcctccttc ttatccaatt gccttcaacg gatcgatttt tacgattgtc cgaacagaaa
     1441 atagtgcgcc taacacagct actaccagca tcaacaagct aatgacgcca aaaaacagcc
     1501 aattgctttg gaatggaacc gcatcaggta gaacaaggga tgttcccact gttcctaata
     1561 gaccaagaag aataccaaca aatgataaaa tgaatgtttg tgccactaca gaacgcgcaa
     1621 taaatcctcc tgtgatccct tgtgctttca ttacaccaaa gatattaatc ttttgcatcg
     1681 tcagtacata aatgaaaatc ccgatcacaa tagccgcaat aacgatcaaa aatccgatca
     1741 taaatccaaa agtcaatact tgcgcattat atccaggtaa ttcattgatg aatttagaaa
     1801 ttttgatctg ttctaaatct tcaggtaaat tactgatttt acctctgaca actattgcgt
     1861 taatccgtgc attttcactt gtatcttctt tttcgaagcg gatctcttgg taagcattga
     1921 tggttgtata gagaactggt gagacattga atttggcatg atccgtaaag ccagttagtt
     1981 taaacgtctt gtcactacca gataacttaa ctgtatctcc tacggctaga ccatattctt
     2041 ctttcaaact aatatcgcct actgcctcat catcgttatc aaaagctttt ccttcaacaa
     2101 gattgggcat gatgaattga tttttatcga taccgaagaa gctaacattg atttttcctt
     2161 cttctgtact gtctttacta gtcacgactc caggtgtctg ggcaagatac gccactttcc
     2221 caccttcgac ttcttctgct atcttcttag taatcatgga catccctaga tttgaatttg
     2281 catctttcga aagcacaatc gaatctgcct cccacttgtc gactgctgtt cgattatctt
     2341 gagctaagcc ataagctaac ccagtcaaaa aaaataccag gtaggcgatc aaaaacatca
     2401 cccctgcaac taacgcataa cgtaactttg aatgcataat ttcatttaat gctaaaaaca
     2461 tttaactcct cctcctgaaa taatcatttt atacaacaat tttatcactt gataaatttt
     2521 tggcgaatat
//
LOCUS       DANKHK010000163         2352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000163 DANKHK010000000
VERSION     DANKHK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            159..2021
                     /locus_tag="PW109_002516"
     CDS             159..2021
                     /locus_tag="PW109_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2512286.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2021..>2352
                     /locus_tag="PW109_002517"
     CDS             2021..>2352
                     /locus_tag="PW109_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2512287.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          847 a          349 c          459 g          697 t
ORIGIN      
        1 aaaaaacgct ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt
       61 agttaaaaag ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt
      121 agaaaacgct tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag
      181 aaaggcagct tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg
      241 ctgaaaaaga aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt
      301 ggctgaatga gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata
      361 ttagtagctc tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa
      421 tatttgcaga tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag
      481 aagctattac tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga
      541 atgatttaga tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg
      601 cgcgaaaggg tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga
      661 ttcttcaaaa agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg
      721 gcggtgagac tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat
      781 ttaatcaagt aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga
      841 attacgcgga attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca
      901 aagaggctac gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc
      961 tttcttatcg cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg
     1021 aatatcatta tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac
     1081 tagttgagaa tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc
     1141 cacaattgtt gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat
     1201 ttgtcaatga atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg
     1261 atgcgatcaa gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt
     1321 ctttcgtggc atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac
     1381 gaagcgttag agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc
     1441 aaaagatcaa tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg
     1501 cgcaagaaat gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg
     1561 gcatcgatcg tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa
     1621 ttcaacaaat aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga
     1681 atcttagaga aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt
     1741 tgaaagctta tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag
     1801 aatatcgaga agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc
     1861 aacaatatat ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg
     1921 taggaaaaat cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca
     1981 gtgcattatt ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc
     2041 tttaaaaatt atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat
     2101 tgaagagcga ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat
     2161 taatcacttg aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga
     2221 tcgggatttg ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag
     2281 aagtatcggt aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc
     2341 aaaaaataat ta
//
LOCUS       DANKHK010000164         2304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000164 DANKHK010000000
VERSION     DANKHK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            7..2160
                     /locus_tag="PW109_002518"
     CDS             7..2160
                     /locus_tag="PW109_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2512288.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          785 a          414 c          513 g          592 t
ORIGIN      
        1 taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt agaaaaaata
       61 agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt tccttttcgc
      121 ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc tagattgggt
      181 tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac ttctacgatt
      241 acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa agctttagtt
      301 acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac ggctaaagat
      361 ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga tgaaaatctg
      421 acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa agaagccaca
      481 aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag tgcgatggtc
      541 aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat tgctaccatc
      601 tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa ctctggtgtt
      661 actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg agtttcgact
      721 ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat tcttggcagt
      781 gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct agcaaaaggt
      841 tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga agatgtcctg
      901 caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt ttcgaatcaa
      961 aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa tgcagaattc
     1021 caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa caacggcaaa
     1081 gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg agaagtgcta
     1141 gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa tgctttaggc
     1201 acgattacta gcgcctttgc tccagggtct gtagtcaagg ctggtacttt gacagcaggc
     1261 tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat tcgtctgcaa
     1321 ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct tgatgcagtc
     1381 aaagcattgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa gatgttagga
     1441 ctagactata cacctggaat gggactacct agtttagatg aagaagctaa agcttatcag
     1501 caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat cgatttgccg
     1561 aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa tgcggataat
     1621 ggcaaagaat ggtatactcc aggtaacttt accgatttgg catttggaca gtttgacaca
     1681 tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg gaaacgagta
     1741 cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt gggtgaagtg
     1801 aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga aaatatggcg
     1861 atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac agcagccaaa
     1921 ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga aacggttgcg
     1981 gaaggtcatc cagatattac gacagtcaat agtaatatcg tggcatacgg accaacagac
     2041 aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca agaccacatg
     2101 aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat ggcaaaataa
     2161 aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt gtgaaaatag
     2221 cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt gaacaccgtt
     2281 tactcggcaa aaaaggggct cttt
//
LOCUS       DANKHK010000165         2294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000165 DANKHK010000000
VERSION     DANKHK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            131..1744
                     /locus_tag="PW109_002519"
     CDS             131..1744
                     /locus_tag="PW109_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2512289.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          716 a          407 c          447 g          724 t
ORIGIN      
        1 ttaggtttta tccacaaatt atttctctat attttctgtc tattcaaaat aaacaataat
       61 tttccggcac aattatttgc tgttttttac cataacatag tacgatcaac gtagaaaagg
      121 agatgttttt atgaaagttg ttgtcattgg cggtgtagct ggcggtcctt catttgccac
      181 tcgtttccgt cgattgaatg aagcgcacga aatcattatc tatgaacgcg gagaaaatat
      241 ttcttatgca agttgtgctt tgccttatta tttaggaggt gtgatcactg accgtgactc
      301 gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac aacatcgatg tctttactaa
      361 acacgaagta acagcaatcg atccttctac taagcgatta acagttaaag acctatccac
      421 aaatgaagaa ataaaaacag attacgataa gttgatcatc tcttctggtg ctagaccaga
      481 ttatccggat attcccggag tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac
      541 ggatgcggat cgaatcaaat cgttccttga agaaaaaaat ccacaacatg tcgtcattct
      601 tggtgcaggt gttatgggtc tggaattagc tgagaatttc aagcatcgcg gcttagacgt
      661 gactttaatc gatcaattgc cacaagtcgc tttcccttat gatccagaaa ttgctaattt
      721 agtttatgac aaattgctga aagaaggatt agccgttcat ttagaaacaa gagttactga
      781 gatccgtgat aaaggtcgag aaatagtatt atcagatggc tccgtccttt ctgctgatat
      841 gctaattttt gctgttggtg tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca
      901 attatcagat acaggtcaga tcattgttga tgaccagtta caaaccaatc tgccggacat
      961 ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg actggtcagc cgatccaaag
     1021 tatgctttcc agtgcggcca atcgtcaagg acatatgctg gcagatattt taaatggtac
     1081 gcctatgcgc tatcgcggat atattggtgc aggtgtcgca aaaatctttg atcatacagc
     1141 aagttatgcg ggaatgacag aacatgaatt aaaagcatca ggcataacaa attataaaac
     1201 tgtttttatc actccctttg accatgccta tttctatcca gaagctacaa gattaaatct
     1261 aaagctgatt tttgatgcag atagcggtcg tattttaggt ggacaagcat ttggagaaaa
     1321 aggtgtcgat aaacggatgg gagaactttc tgtagcaatc accggaaact tgacagtctt
     1381 tgatttgcct gatttggagt tgccttactc tccaccgtat tctactaccc gtgacccgtt
     1441 gaatatagct ggttatgtcg caatcaatca aatgacgaat atcgtagaaa cgatcaaagc
     1501 aagtgatata cccgaaaacg atttgaaaga agcgttcttt ttagatatac gtgaacctaa
     1561 taaagcacct tctggcagta tctcggcaac aaaaaatatt ccaatgaatg agttacgaga
     1621 tcgaatcaat gaaatcccaa aagataaaaa agtttacatt actttcagaa gaggattgaa
     1681 tacttatact tctgcccgaa tcttggcagg tttgggtatc aaagcgattt tgattgaaga
     1741 ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt tatttttact tgaggagtaa
     1801 aaatgaaaaa gtatataggg ttgttggttg gtatgtatat agaatagaag ataaatgtga
     1861 agaaattgtg cattatttct tcacatttat taggttatag tttaagaaag tttaaacatt
     1921 gcgcaattta attattttag ctgtttcttt tcataaaaaa acatgttgtg actctgagac
     1981 aaatcatagt ccatagtgat gtctctagag taactacgcc taatattacg aggcatcata
     2041 ttaatctatc agcactagta cctaaactag catccctttc gaatattttt tcttatataa
     2101 taaagtttgc tttcactcct ctaatctcac caaatcgact ggcttgcgtt gtgtgatact
     2161 aggaactgat tggtttagaa cgacttttct attgtaatca tatttttcgc catcctcgtt
     2221 gtcttctaat ccttcaatga atgatcccta atgtgtgtat taaataattt aatttttttg
     2281 ttcctattta ttta
//
LOCUS       DANKHK010000166         2276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000166 DANKHK010000000
VERSION     DANKHK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            794..1111
                     /locus_tag="PW109_002520"
     CDS             794..1111
                     /locus_tag="PW109_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512290.1"
                     /translation="MEKTKKLQLEDFTENEFFGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            1645..2142
                     /locus_tag="PW109_002521"
     CDS             1645..2142
                     /locus_tag="PW109_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2512291.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGNEEEMKILNNEYDE
                     LFSKLKNTHKNKDGETYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKIKEEKEEKK
                     YLQAN"
     gene            2177..>2276
                     /locus_tag="PW109_002522"
     CDS             2177..>2276
                     /locus_tag="PW109_002522"
                     /inference="COORDINATES: protein motif:HMM:NF025570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512292.1"
                     /translation="MFYKIRIIETLEKEFEIEAKNEKEALEKIQIAY"
BASE COUNT          927 a          393 c          368 g          588 t
ORIGIN      
        1 ataccaacat gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc
       61 aattcctatt atacaaaatt tttaatgcac tatcaacaca ctcttaagtt tgcttctaag
      121 tcttatttcc ataactttag ggttaaccat acgcaagacc aatcactctc ggacaatact
      181 catgatttta atgataaaat ccatgttaaa cccatagata agaaatacac ctgcaataac
      241 cgttacctgt ttgtgccaat ttaaaaatgc accacttttt tataattaaa acggttgaaa
      301 actgtaccac taataactca caatagagag atgtcaccgt caagttaaat gtacaaaata
      361 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
      421 ccatgtttta aaagctgatc gagaaataaa acacacaacg gtattctttc ttttttcagt
      481 tggtacattt tgcagtctgt aacgtgtcat ttactgtttg ttaccttaac atatccagtc
      541 gaaaacacaa gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt
      601 gcgtactcgt cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa
      661 aaagaaagga gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt
      721 cacttcctca aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaaagaaa
      781 agaggaaaag aaaatggaaa aaacaaaaaa attacaatta gaagatttta cagaaaatga
      841 gttttttgga acacaagaac agcaatatct aaaagctcaa gtaagagaag agctaaaaga
      901 gcaaggcttt atcatagata gcagttttga gggcgatttt aaaacatgga taggtgtata
      961 cgcaagacca aaagataaac cgacctactt agaccctcaa aacgataaag aagcagaaga
     1021 acaagagcaa tattctatta acggctttaa acaagatttt agcgaatggt ttgagtggga
     1081 aataaaaaat ttgaaaatta aagaaatgta aaagaaaagc ctagcaaaaa aagctaggtt
     1141 tttcttttag ataaaacacc aaaaagaaaa acctaagaag gataaaataa gttgttccac
     1201 ttgcggtgca gttgctcctc gttccaaacc caacttattt ctttgcaaag atatttgtat
     1261 ttgactaaca cctttcctcg aatggtaaaa gctaaccgtt cgttactcta cctcagatac
     1321 tttctttttt cagttggtac attttgcagt ctgtaacgtg tcacttactg tttgttacct
     1381 taacatatcc agtcgaaaac acaagggtat ataaacgagc aagctcgccc ttgtgttttc
     1441 tttgtgtcta tgttgcgttc tcgtcacagc aaacgacacg aacacactgg cgaaaatgaa
     1501 ccacctaaac gaaaaaagaa aggaacaaga tatgtagaac actcacagct tttaccattc
     1561 gaggaaaaca gcttcacttc ctcaaacgcc taacaaaaat ggttgtcaaa aagccaaaaa
     1621 taaaaaagaa aaggaatgat aaaaatgaaa tacattaaaa acgttgaaac attggaagaa
     1681 ttgaaaaaag cctacaaaaa gttagctttg aagttacacc ccgattgtgg cgggaatgaa
     1741 gaagaaatga aaattttgaa caatgaatat gacgaactgt ttagcaaact taaaaacact
     1801 cacaagaaca aagacggcga aacctataca aaagaaacga ctgaaacgcc cgaacaattt
     1861 aaggatatta ttaataaatt gttcaatcta aaaatggacg gcgtagcaat cgaagtagta
     1921 ggaacattta tatggctaac aggtaataca aaaccttata aggacgacat aaaagcccta
     1981 gaatttcgtt attcaccaaa aaaatacgca tggtataaag caccaagcga ttataaaaaa
     2041 cgtagtcgaa aaaattatga tatggacaca ataaggggaa tgtatggaag ccaaaaaatt
     2101 aaagaagaaa aagaagaaaa aaaatactta caagccaatt aaggcttgta agtttatcag
     2161 aaaggaagaa accattatgt tttataaaat aagaataatt gaaactttgg aaaaggaatt
     2221 tgaaattgaa gcaaagaacg aaaaagaagc attagaaaaa atacaaatag cttatg
//
LOCUS       DANKHK010000167         2271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000167 DANKHK010000000
VERSION     DANKHK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            130..1629
                     /locus_tag="PW109_002523"
     CDS             130..1629
                     /locus_tag="PW109_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2512293.1"
                     /translation="MKKTFISTSVIVFSLFFSNGLLAKAQTIEEKIKEKDDKIEAIVK
                     NKESAENYLTDLDNKITQLEKEYKKVLVEKNTSEKKLTEINQKITALEERIRLRNKLI
                     EEQARNAQVNQKSDSIISVMVNAESLSDAVTAAIGMTKLISASNEIMQAQIDDKENLD
                     ELKKEAEKKVTDAEKEIRSLEEKENDLIEAKLTQSIKINEISASLATEQSEKAKFENQ
                     KQEAEKRREEELKAIAEEKRKEAEAAAILKAQEAEAASIIFENQKQEAEKRREKELKA
                     IAEEKRKEAEAAAILKAQEAEAASIIKDQEKDVSQLADTNDIGIGQENTDTQKEEQIP
                     DETSEQDSNSVNQNEYQDNNSSSDNTQIQSPSSSGWGAPVANIIVTSPFGGRADPTGF
                     SGSFHNGIDMGGTSSTPIMASRSGTVVQASFDGSAGNYIIIDHGDGYYSYYLHLSNYI
                     ATPGQSVSAGQTIGTMGTTGNSTGVHLHFGIATSSNWSGFVDPAPFLGI"
     gene            1632..2271
                     /locus_tag="PW109_002524"
                     /pseudo
     CDS             1632..2271
                     /locus_tag="PW109_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
BASE COUNT          885 a          358 c          424 g          604 t
ORIGIN      
        1 tcctattact ttttgtatag taattccaat ttcaattcct aatagaacct gtagcaacga
       61 taaaatagag aggagaatat tatgtaaaat gtttcttcac aaataaaata agggaagtga
      121 aaaatcaatt tgaaaaaaac atttatatct acctctgtca ttgtgttttc gctatttttt
      181 agtaatggtc tcttagctaa agcacagact atagaggaaa aaatcaaaga aaaagatgat
      241 aaaattgaag cgattgtaaa aaataaagaa tcagctgaaa attatcttac tgatttagat
      301 aacaaaataa ctcagctaga aaaagaatat aaaaaagtgc tagtggaaaa aaacacatct
      361 gagaagaaac ttaccgagat taatcagaaa attactgctc ttgaggaaag aatacgcctt
      421 aggaataaac ttatcgaaga gcaggctagg aatgcacaag taaatcaaaa aagtgactct
      481 ataatatctg tcatggtaaa tgccgaatca ttatcagatg cagtcacagc agccattggt
      541 atgactaagc ttatttctgc tagtaatgag attatgcagg cccagataga tgataaagaa
      601 aatttagatg aattaaaaaa agaagctgag aaaaaagtta cagatgcgga aaaagaaatt
      661 cgatctctcg aagagaaaga aaatgacctt atagaagcca aattaactca aagtataaaa
      721 atcaatgaga tatccgcttc tttggcgaca gagcaatccg aaaaagcaaa atttgaaaat
      781 caaaaacaag aagctgaaaa aagaagggag gaggagctta aagctatagc agaggaaaag
      841 agaaaagaag cagaagctgc ggctatttta aaagctcaag aggcagaagc cgcatctatt
      901 atatttgaaa atcaaaaaca agaagctgaa aaaagaaggg agaaagagct taaagctata
      961 gcagaggaaa agagaaaaga agcagaagct gcggctattt taaaagctca agaggcagaa
     1021 gccgcatcta ttataaaaga tcaggaaaaa gatgtatcac aactagccga taccaatgac
     1081 ataggaatag gtcaagaaaa tactgataca cagaaagaag aacagatacc agatgaaact
     1141 tcggagcaag attcgaactc agttaatcaa aatgagtacc aagacaacaa tagttcttcc
     1201 gataatactc agattcaaag tcctagttct tctggttggg gcgcacccgt agccaacatt
     1261 atcgtcacaa gtccatttgg gggaagagct gatcctactg gattttctgg atcttttcac
     1321 aatggtatag atatgggtgg aacttcttca acgcctatca tggcatcaag atcaggaact
     1381 gtagtgcagg cgtcatttga tggatccgca ggtaattaca ttattattga tcacggagat
     1441 ggttactatt catattacct tcacctaagt aattatattg ctacaccagg ccaatcagtg
     1501 agtgctggac agaccatcgg taccatggga actacaggaa attcaaccgg agttcatttg
     1561 cattttggaa tagcgactag ctcaaattgg tctggattcg tagatcctgc tccatttcta
     1621 ggaatctaaa aatgatgaaa aaaatcaagc atctttcttt aaatttactt ctaattagcc
     1681 tttttcttat aggcttagtt ttaatattta atttccaaat tcgatcttac ttactgaata
     1741 aacaagtcca caatatgaca tagcaaatta ttcacagaaa gatttggaag acaatctgaa
     1801 aaggaaagga aactttgatt ttgattctgt tgaatctata tcaaatgagt tagtattaaa
     1861 agcacaattc aagaataatg atttgcctat aataggtggt atcgctatac acaatgtaaa
     1921 attatcttta ccaatataca acggattgga caacacctcg ctcctttctg gggctggaac
     1981 aatgaaacca gatcagcaga tgggaaaacg aaattacgct ttagcaagtc atagtgctaa
     2041 agataaaact attttatttt ccccattaga gtttatttct ctaaaagaaa aaatatatgt
     2101 aacagactta aagaatattt atatttacca agtgtccttt aaagaaaaag tggatccatc
     2161 aagagtcgaa gtcattgatg atattcctaa taaaaatatg atcactcttg ttacatgcgg
     2221 agatttggct gctgtaacga gattgattgt acaaggtaca ctgatcgata c
//
LOCUS       DANKHK010000168         2252 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000168 DANKHK010000000
VERSION     DANKHK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            2..1375
                     /locus_tag="PW109_002525"
     CDS             2..1375
                     /locus_tag="PW109_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512294.1"
                     /translation="MISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAI
                     ALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFL
                     SKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSIS
                     DSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPW
                     FLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTS
                     IAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKV
                     GESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVN
                     VDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEI
                     NKKNENK"
     gene            1362..>2252
                     /locus_tag="PW109_002526"
     CDS             1362..>2252
                     /locus_tag="PW109_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512295.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLIL"
BASE COUNT          745 a          339 c          388 g          780 t
ORIGIN      
        1 tctgattagt ttttattatg gagaaaagtt ttttaaaaag atagatgctg cttttttatt
       61 tagttgttta tatacttttg caacttatcg aacagttgat atctattatc gatccgcaat
      121 tgcagaagca atcgctctta cgattatccc agctgtttta tattatgctt atggattgat
      181 attcaataga gaaaaaaaag tcatcgcttt atctgtcagt ataagtctac ttgtgtatac
      241 tcatgtctta actacactat tgtgtagtgc attgattgct tgtatgatta tagttcgttt
      301 gtttttaagc aagcaaataa aaaacgatat aaaatttgtg gtcgtgaatt tagtaaaggc
      361 tgtttttttg acttgtgcat taactagcta tttttggtat cctatgttgc agcagatgaa
      421 ttatcaagaa atacacagtc ccttcaaaac agatttgcag ctagaagcac tttctatttc
      481 agatagtatc attcaagcgt taaataacga tattacaaca tacactatgg ggttattagg
      541 attggttgcg ttgatttttc ctttattcat actgaaagaa atggagaaaa aagaaaaaat
      601 catctatgtt ggcgtagttt ctacatgggt atgcgtaact aatttatttc catggttttt
      661 gtttcaaaaa acaccgataa gcttactcca gtttccttgg cgtatcttag gtatccaaat
      721 ctttttttct tcaatgatca taacgcttgt ttttatgaaa aagactatga ataaaaaata
      781 ttcatggtta atcattggcg ctactgtggt gtttctactc gtcacgtcta tagcagcaaa
      841 agaaaattac gcaaaagtta tccaatcaaa acacggtcat attgttatca atgaagaaac
      901 actcccacgt tatacaacat cagatatggg aggattatac gattacgccc caacagaagc
      961 gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa gtaggagaat cgtgggaaaa
     1021 agggaagtat aaagctgcag ataataacat tacttacaca gtggcaagca acaaaaagca
     1081 aaaagtgaca gtaccagtat atgcttattt aggtacacag gtaaaagtaa acggtaaagt
     1141 cgtaaatgac tcttataaaa aacatggctt agtgaatgtg gatgtaaatg ctggagaaaa
     1201 taagatagaa attacaacaa aatatactcc aactgctctc atggctttat ggatcagtat
     1261 tctgacttat tgtttagtgg tgtattcaag ttttaaacga tttgcaccta ataagttgaa
     1321 agattctcga tatgtgacta aaaaggagat aaacaagaag aatgaaaata aataaaaaag
     1381 tagtaatatt gagtggatta attatagtta gtagtatata ttctttcatc tttttcaaaa
     1441 atacacagag tatttatacc actggagatc tttcttttca cttatctcga atcaaggggt
     1501 tatcgactat ttttaccagt ccgattaatt atgagacatt caattatact ggatatggtg
     1561 taaattattt ttatcccttt ctgacttttt ttccagctgt aatgttatat tggatgacaa
     1621 aaaatttgat tgtaagctat attatttacg tttggttatt gaacttgtgt acaacactcg
     1681 tagcttatca ttatggcgag cggtttttaa aacaaaaaaa agcggcattt ttatttagtt
     1741 gtctctatat tttctctgct tatcggacag tagacatcta ctatcgttcc gcaattgccg
     1801 aagctatcgc aataacactt gttatccctg tattatttta tgcttatcaa atcatctctg
     1861 gaaaagaaga aaagtatccg tctgtaaagc tagcattgag tatgagtttg ctagtgtaca
     1921 gccatgtact atcaacatta atgagtacag cattaattat catttttatt tttattcgtt
     1981 tggtttctaa aggtttcaag aatgcagatt ttattgctat atttaaaaaa ttatttagtg
     2041 cggcaggaat gacattggtt ctgacgagtg ctttttggta tccgatgttt gaacaaatgc
     2101 tctaccaaaa aatcaacaaa cctagcgtga caaatttata tgctcatgca tctaatgtgt
     2161 tcgatagtct gactgaagct atgaacaacg acttaactac ttactcaatg ggactagttg
     2221 gtctgttatc tttatgtatt cccctgattt ta
//
LOCUS       DANKHK010000169         2159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000169 DANKHK010000000
VERSION     DANKHK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            59..493
                     /locus_tag="PW109_002527"
     CDS             59..493
                     /locus_tag="PW109_002527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512296.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSKLCCASYADVEDAV
                     PLNHVRRCSFMSRKAGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(554..919)
                     /locus_tag="PW109_002528"
     CDS             complement(554..919)
                     /locus_tag="PW109_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512297.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(995..1240)
                     /locus_tag="PW109_002529"
     CDS             complement(995..1240)
                     /locus_tag="PW109_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512298.1"
                     /translation="MSIEETILLDTLRCRLNFLRTGRSLSQVAVSNALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASRLK"
     gene            complement(1310..1563)
                     /locus_tag="PW109_002530"
                     /pseudo
     CDS             complement(1310..1563)
                     /locus_tag="PW109_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1727..2050)
                     /locus_tag="PW109_002531"
     CDS             complement(1727..2050)
                     /locus_tag="PW109_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512299.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMVFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          822 a          361 c          374 g          602 t
ORIGIN      
        1 tctgaaaaca aaaaaagaga gcgttattat tttagtctat tttcaggggg gtaaccttat
       61 gataaaaaaa ctatttttac tatcaatatt aacgctagca atggcaggtt gttctgactc
      121 tggcttagca actgaactga tcacaaaatc aactgaagcc acgacagagg agttaaaatg
      181 tggaaacgat cacagtgact atagaggctt aaatcgtcga tgcatgggaa tattaggcag
      241 aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt agaagaagca acagaaaaat
      301 caaaagaaag atcaaaagaa gcaaactgtg ctgtgcatcg tatgcagacg tcgaagatgc
      361 ggtaccttta aaccacgtca ggagatgttc atttatgtct aggaaagctg gcgatgaacc
      421 aaaactagag tacgctttta gttttgcacg tgaggatgaa gataacccta aaacttacga
      481 cattttagct tgatctaaga tggttaaagc tttttaatga agtaagagga gatagaaggc
      541 ttttctagtt atattaggta agataaattc ctttttgctc caaatttgct aaacattctg
      601 ctagataaac ttcatcgtgt tccggataaa tcggcacgtt gcgctctaca gtaggtaaat
      661 caaaaaatgc ttcgcatgat ttgccacgat acataggatt tttccactct ggtgctgggc
      721 tgtaaccacg tttttccatt tcatccatga caagctgatg atattggaat aatctataag
      781 gagaatagtg aaaaacataa tccacagtag catgtttctt tccccagcca tttcctctta
      841 atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt
      901 tttcatgcca taatcgcata gaaatatctc ctttatatat agattgttaa ctttagacgt
      961 taaatgaaaa aaccagcacc aaggctggaa aaaatcactt aagtctgctt gctgtttctg
     1021 agcgaccaat taggtaatca acggaaatat cgtaaaagtc agcgatatag attaattcaa
     1081 tgatgcttgg aatttcttct ccttcttcgt acttacgata tttgaataaa ggaatactta
     1141 acgcgttaga aacagctact tgagataaag atcggccagt tcttaagaaa ttcagacgac
     1201 aacgtaaagt atccagtaaa atagtttctt cgatactcat ttaatcacct ttcattatag
     1261 tagaatagtt ataatgacta tagaaagcta cacatgcaat caatagtact caatgttttt
     1321 tatttaaacg tatcaaataa agaatgacga tcaataaaga caaaaccagt cctgataatt
     1381 cttctataaa aacactatcg ggttcaaagt agttgtaatt aaaaactttc cacataaagt
     1441 agatagccgg aagagtcaaa atagctaaat aaaaccgttc tttgttcaat tcataagata
     1501 aacaatactt gcgaccagtg cgaccgcagg cacccaaaac aaaactctca ccaacgtaat
     1561 catgtaacgg actcctcaac gattcatata ataaaagata acatgttatt gcatacatca
     1621 caaatgaaat gctaggaaaa taaaagaaga atagatcaat gaaaaatgaa aaagcagaac
     1681 agttttctgc tgataagcag gaactgttcc gctgaatgtg tcttatctat cgagaaaaaa
     1741 tcaatggatt gatatctaag taattcaata catataaaaa gaggacgatc gcattcacta
     1801 aaaaggaaag aatgatgatc ccatagcgaa tggaaacata tttaacaaat acttttgttt
     1861 gaatacgggc atatcttttt cctttatagc tgagcatcat gacaagaagc aaaagggtaa
     1921 tcacaccttg aaaaaccatt ccaataaaca gaattcctga ggtttgataa tttaattgga
     1981 gaaaataagc tgctgccgca actaaatcga ataaaatcgc aataaatggc aaaataatca
     2041 ctcctaacat tgagaataaa taaaagaata acattttttc taaaataagc aaataaaaat
     2101 taaagtaaca aaatagttat gaaacaagta aaagaaataa gaataacaat attatttta
//
LOCUS       DANKHK010000170         2108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000170 DANKHK010000000
VERSION     DANKHK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(98..967)
                     /locus_tag="PW109_002532"
     CDS             complement(98..967)
                     /locus_tag="PW109_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2512300.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(987..2108)
                     /locus_tag="PW109_002533"
     CDS             complement(987..2108)
                     /locus_tag="PW109_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2512301.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
BASE COUNT          623 a          467 c          341 g          677 t
ORIGIN      
        1 gggtatcatg agtgaatgat attgctgaat acaaatagtc cgaccacatt aagttgatcg
       61 gacttcataa gcattttatt gactaatact tttttgctta agaattgatt ctaacatctt
      121 ttgctaaagt taagcatcct atagttcctg cattatctgc taacttaggt gtgacaatgt
      181 attcttcgat atttggcgta tcgacatagc cgttaattag ttctaaaaaa cgtctttgta
      241 ctttcttttt cagatgttct tgttgcatga caccaccacc aaaaattata atatctggtg
      301 aaagcatcag ggtcgtatta caggcacatt gtgcaatata atccgcctcg atttcccaga
      361 acggatcttc tatagaaaga tcttgtccct tggttcccgt acgcatctcg atagcgggtc
      421 cagatgccaa cccttcgaga caatcatgat gaaaaggaca agtaccagag aaagtatctc
      481 ctgaatgtct cttaactaac gtatgcccca tttctggatg gctaaaccct tcaacaaaaa
      541 tcccatcttg tatagctcct ccaccaattc cagttccaac agtgtaataa acaacactgg
      601 ataaaccttt tccatgacct gaaacatact ccccgtaaca agcagcattc acatcggttg
      661 tccaggcaat tggaatttcg aaatgcttat tcaacgttcc aacaaaatca aaatttttcc
      721 aggcagtttt tggagtagag gtgatatgtc cataggtctt tgattcacga tggatatcaa
      781 tcggtccaaa tgaccctact ccaatgcttt ctaattgttc tttatagtga ttaaagaaat
      841 caattaccaa actcattgtt tcattcggtg tagttgtagg aaaactcact ctttctaaaa
      901 cctttaaatc atcattgcca atggcacaaa caaatttagt cccacctgct tctattgagc
      961 catacatttc ttactccacc gtccttttat ctaggaattc gttcttccct gtctaatagt
     1021 ttttcgaaaa actcatgtgg ttccttttgg taccaatagg caacagaaga aatatcgtct
     1081 tgacgctcat aaagcttgat gtcatcattt cctagggttt ggaaagtaac acgtaaattt
     1141 tctttgaaac taatggggtc gggtaaatgc cagcggtaca acccatgcat cggtaaagca
     1201 tcatcgccaa aaccatgaac tggatttgga ttaggttttc cagcactaaa ataatcccgg
     1261 gtgtgatctc ttttagattg aaaaggatag cccataaata gcgtattaaa attttgagca
     1321 tatgcccttt ctccaaagtt ctttttatgg aaagcccaag cgccgccaaa ataatcttct
     1381 gctcctgtag atgttattgt agggaaatct tcatctccat ctatataaaa cttaaattct
     1441 ccttcccccc accagtatct ttccaatgcg cacaaagcaa agtaggttcc tacataatac
     1501 ccagtgcctt tgattccatc aataagggtg taatcttttc cttttgtcgt gatgggttca
     1561 cggttccatt tagcatgaaa atacatcgca tcatccggta cttcagtcaa tgcataatta
     1621 aatgtataaa aaatgtgctt aataaactca ggatgttcat tcgttaaagt aatcttagct
     1681 ttttttcgaa atggcatctc aaaatagcaa ttcatccctg atgttggatt cactacgatt
     1741 ggtaaggaat tcacttcgca tcttgtccca aacccattac aaaaaaaatc tcctaatgga
     1801 ctttctacag aaggagtgga ttcatcatcc caataaatac gcaaaattaa atcacgtaag
     1861 acgaaactcc cagcgattgt tttgtctgtc actgtcatcc aaaaatgacg taaaattcca
     1921 gggccttcca tttctgctaa agtaattgtc tctccagcat ttatcggaac agaaggtgac
     1981 ccttttctcc caattcctag atgactcgct gtcattgccg ctttcccttt ttcaccattt
     2041 ttattttctg gtgaaatagt tctactttcc tctcccttgt ggagccatat attatttaaa
     2101 caattcat
//
LOCUS       DANKHK010000171         2094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000171 DANKHK010000000
VERSION     DANKHK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(119..709)
                     /locus_tag="PW109_002534"
     CDS             complement(119..709)
                     /locus_tag="PW109_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2512302.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="PW109_002535"
     CDS             1232..1393
                     /locus_tag="PW109_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2512303.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="PW109_002536"
     CDS             1412..1906
                     /locus_tag="PW109_002536"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2512304.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1899..2060
                     /locus_tag="PW109_002537"
     CDS             1899..2060
                     /locus_tag="PW109_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512305.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          750 a          331 c          360 g          653 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttt
//
LOCUS       DANKHK010000172         2055 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000172 DANKHK010000000
VERSION     DANKHK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..>2055
                     /gene="acm"
                     /locus_tag="PW109_002538"
     CDS             <1..>2055
                     /gene="acm"
                     /locus_tag="PW109_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2512306.1"
                     /translation="LIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKK
                     QNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNL
                     DSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGD
                     MLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRG
                     EEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENH
                     SEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFE
                     LRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKF
                     SINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAE
                     TKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNEN
                     KEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKY
                     RDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTERE
                     KIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVII
                     GLL"
BASE COUNT          804 a          339 c          405 g          507 t
ORIGIN      
        1 ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg
       61 ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt
      121 gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa
      181 ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat
      241 gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa
      301 aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat
      361 accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta
      421 aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac
      481 atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa taacggcact
      541 tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca
      601 agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag
      661 ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat
      721 aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt
      781 tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg
      841 tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat
      901 atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat
      961 atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa
     1021 cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt
     1081 atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt
     1141 gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag
     1201 ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt
     1261 tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca
     1321 aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca
     1381 gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg
     1441 caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac
     1501 gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg
     1561 gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc
     1621 gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc
     1681 acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac
     1741 gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa
     1801 aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag
     1861 acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca
     1921 ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata
     1981 acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta
     2041 atcattggtt tattg
//
LOCUS       DANKHK010000173         2012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000173 DANKHK010000000
VERSION     DANKHK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(56..1691)
                     /locus_tag="PW109_002539"
                     /pseudo
     CDS             complement(56..1691)
                     /locus_tag="PW109_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          355 c          313 g          656 t
ORIGIN      
        1 gtttattact tactttttct tattctctag taagctaata ttgaaaaaaa tgtaatcaga
       61 tcattatttt tatatgttgt aacttcctga taagaaaact aattaaaaga aagaggtaac
      121 tacataaact aattaaaaaa gctaacttca tagttggagg ataaatggaa gaaacttcaa
      181 tgattgtttc tccttgtgtt ccttcaatcg acactagtcc tattatcaat ctctcttttt
      241 tgtccattta cttttgcgac tgttccccaa taatcaaata caggaagcgt tattttttgt
      301 cctttagatt catgtactgt atagactatt ttcgaatcca tcacttgata aggcatagta
      361 tgccattcct tgccaattct tacttcatga ttctgcaaat gttctttgta ttgtaatgct
      421 tccgtgggtg catagtcata aagtcctccg ggaaggagtg tcgtgtaacg aataagattt
      481 tcttgattca ttaccaagtg atctctctga gtgacaactt tatgttcata gtttgctttt
      541 gttgcagcag atacagccaa cagtaataaa acaattgccg ttatattaac tacttttctt
      601 tgcttagctg atgtgctctt catgaaaacg aacgataaaa tcaacgagcc aaacagtact
      661 tgaaagccta agattcgcca aggaaattga atcatttgaa tgggtgtatt ttgtaataaa
      721 gaccatggaa aaaatttggt ggataagagc caagagacta ccgcactaag gtaaatcact
      781 ttttctttgc gattaaatcc tttaaaaaag aagagtggac agatcaaaga aataatacca
      841 atgattccta ccgtaaaggt tgtcaggtca ttattcatcg caccaatcaa actatccgag
      901 acatttaatg cttggtgttg taaggtcgtt ttatctggct cattaatcga ttgaaccatg
      961 atttgtcgga acatcggata ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc
     1021 tttgacaact ccacaatcaa ccggatgctt tctttttttc ttgtttcact tactacaaag
     1081 ccgcctaaga ctagtaagcc aacggctatt acactcataa aagtggaaag aacatgtgta
     1141 taaatgagta agctcatact gactgctaat ggaattgcac atggcttttt ctcataaatc
     1201 agtgcgtaag cataatacat aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata
     1261 gcagaacgat gataaatatc aatcgtacgg tacccataaa atgtatataa acagctaaat
     1321 ataaaagccg catcgattct ttttaaaaat tttagtccat aatgaaacat cagcatgata
     1381 gtacaaacat tcaatagcca aacatataag atataactaa cgattaaatt attacttatc
     1441 caataaaaaa tgactgccgg aaaaaaggtc aagtagggat aaaaataatt cactccgctc
     1501 ccataatgat taaacgtcgt aaaattgatt ggaccagaaa aaattgaaga taaccctttg
     1561 actctagcaa aatggaaggc aatatccccc gatgtagaaa tagtatgaac atttctaaaa
     1621 taattgattg aataaagtcc agcaataatt gctaggagaa tggacaatat tatttttttc
     1681 tttacttgca ttacttaatt atatactcct ttcatggatt gctttatcta gtataactta
     1741 aattttaaat aaatcgttat ttatttttac tctcacttaa aaaagcacta aaattataag
     1801 agagggaggt aatggtctga acaccaagaa ttgttccaac atcgaattgc ttaactaatg
     1861 agttcaaagc ttaattatgt ggctcttata tttaccgagc gtggattgcg atctctttag
     1921 cgaaacaaac atctttcata aaaaaaacag ccatatccgc tttcttggat atgactattt
     1981 actactactt tactatcttt cattcttctt gt
//
LOCUS       DANKHK010000174         1993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000174 DANKHK010000000
VERSION     DANKHK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            797..1270
                     /locus_tag="PW109_002540"
     CDS             797..1270
                     /locus_tag="PW109_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2512307.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNMLKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1279..1506
                     /locus_tag="PW109_002541"
     CDS             1279..1506
                     /locus_tag="PW109_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512308.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          648 a          329 c          302 g          714 t
ORIGIN      
        1 attcattgat cataacgatt ggctatttga ttgtatcaaa tctaggaaat atggtatttg
       61 gtatcagtaa agaattcagt tggactacta cattatggga atctctattc tttttcatct
      121 tcgtattttt gttacaaaat tatcgaaaaa aataaacatc taccatctat ctgttggcac
      181 atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc tacttcttct
      241 taatacctct aacacttttg atttataaac taatccaatg catctacttg cgcttattaa
      301 gctatttcag ctagatagta ccacacccca tgcgtcaatg cttgattttc cttttgtata
      361 acattgtata agcaataaag gacaaataag tgatgattac accaaaccat tgaataagaa
      421 aaattctgat acgtaaataa acatgaactt gaaaaaatta atgaatcggt tagctactac
      481 gtagttgtct tttttctcat acagttctgt taacttacac ttctcttcgc tagagaataa
      541 aaaatcctcc aactgtattg tccaatataa tcccaggcat ctaaactagc attatagaag
      601 agacacacat agaatctaaa ataactggtt ttaaatgttt cttacaatca aaaaaagctg
      661 ttaaatgcac tccacaccct catactctat cattttttat aaacgatata tttttattgt
      721 ttttcgataa aaaacagttg ttttacaata attattctgc tatattttag ctatcaaaga
      781 taaaagaggg ataaaaatga aatttaaaac attgctatta aaatttgtcg ttttaggtat
      841 aagtgccgta tttattttgt tcggcgtact tttttacact cagatcgcta caagcgaaaa
      901 aggattcaat tttgattggc agaccacatt atttgtttct gtcatttact ttgcccttct
      961 cctagggatc gttattgctt atttcttatt gaaactattg aaaaaaatcg aagataacca
     1021 agctttttcc actcaaagct taaacatgct aaaaaaaatc gaacgaacca ttttgtttat
     1081 tggtttcgct ttttttggct tacttcctaa atttatgaaa gtagcggatt tagacgatgc
     1141 tccaggtttg atgttggtag gaatcgctgt cgtattcatt ccttttgcag tatacacatt
     1201 aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta cttaagacag atcatgattt
     1261 gacagtgtga ggtgcgacat ggcaattatc gttaatttag atgtagtatt agctcaaaaa
     1321 aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta cgatggcaaa catctctatt
     1381 ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc tcgaaaaaat ctgccaagtt
     1441 ttggagtgtc aaccgggtga tttgttgacc tataaaaatg actcgaatac tataccagaa
     1501 gaataaaata aagtagaaca aacagtaaat aaaaaataat ctgaatgata gatgtgtgag
     1561 acttttagaa gttatttacg tctattattc agatttttta tttcgattga ataccgtttt
     1621 atctataaat aagatatgta ctgactcata caaagggggc cttttcttct ccatgtacta
     1681 tcacttttcc tgatacaaca ggtttcctgt tacgttgtag ttggatctga ttttagtata
     1741 atactttatt cattccacca gactgatagc agtttgagca gtctaaataa tcatgtagta
     1801 ttacaggcca ttctgtttca taccttgaag tgcttaaaac ttcagctaat ggcccattgt
     1861 gttaactctt actccattct tttttagtaa actcccatat atactgtaaa gccttctata
     1921 tattatgtca tgttgctttt taccatttac tatgtagttt tgataaaatg atttgaataa
     1981 cagtgatgga cta
//
LOCUS       DANKHK010000175         1971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000175 DANKHK010000000
VERSION     DANKHK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            120..1313
                     /locus_tag="PW109_002542"
     CDS             120..1313
                     /locus_tag="PW109_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512309.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1479..1901)
                     /locus_tag="PW109_002543"
     CDS             complement(1479..1901)
                     /locus_tag="PW109_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512310.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          520 a          326 c          409 g          716 t
ORIGIN      
        1 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
       61 attctttttt ggatatactt ttaataattt agggaaattt taggaaaagg ggtataaaaa
      121 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
      181 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
      241 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
      301 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
      361 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
      421 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
      481 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
      541 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
      601 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
      661 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
      721 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
      781 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
      841 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
      901 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
      961 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     1021 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     1081 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     1141 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     1201 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     1261 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     1321 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     1381 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     1441 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     1501 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     1561 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     1621 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     1681 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     1741 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     1801 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     1861 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     1921 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg t
//
LOCUS       DANKHK010000176         1939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000176 DANKHK010000000
VERSION     DANKHK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            2..1903
                     /locus_tag="PW109_002544"
     CDS             2..1903
                     /locus_tag="PW109_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2512311.1"
                     /translation="MIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIETWLRDKHL
                     PLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLVAKDGFSL
                     EELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQLLVKTLR
                     NMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIYFMLERMK
                     QYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRIANLFTNS
                     QAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLHYGLQISN
                     PLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERHKHQKSRR
                     YQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFILATLPFK
                     HISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQLERTDPA
                     DILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKHSMISIAT
                     MKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLMQSNHPLE
                     ILTLLSSYE"
BASE COUNT          701 a          346 c          340 g          552 t
ORIGIN      
        1 tttgatctat caattaagtg agaacaataa ttatataaca acaaaaaagc tagcagagca
       61 tttccatgtg tcagaaagaa cgattcatag cgatttgaat accattgaaa cttggttgag
      121 agacaaacat ctgcctttaa tgtttgaaag aaaaaaaggt acaggtatct tgttgaatgg
      181 tctgcctcac gaaagggaac aactgaaacg agcgcttaat gaagagactc aaacagaaat
      241 ggataaaaaa cagcttcgtc agttattgat tttccatctg ctagtagcaa aagacggatt
      301 tagtcttgaa gaactttcag aaaaactata tgttggaaaa cgtacgattc gtaaagaatt
      361 gacagagttg caaagctttt ttgagtatca ccatttacag ctagtatcca aaacaaaact
      421 aggaactttt cttaaaggcg atgaacaaga aaaaagacag ttgcttgtta agacattacg
      481 aaatatgcag aaagaagatc cacaaagtcc gtcgttgaaa gaattcttcg ctaaagatac
      541 attaaaagtt atccaacata cattacaaga tgttttttat gaaaaccatt tagaacagcc
      601 gaatggactg gctagtgtgg agattcacat ctactttatg ctcgagcgaa tgaaacagta
      661 ccagaaagtg aaattaagta aagacgaaaa cgaagtcgtg gaacacaccc aagcacaaaa
      721 gttaagtagt caaatattag caaaactagc caccatctat ccaattgaat tttctcctga
      781 tgaaatcaat tatctggctt tgcgtatcgc caatcttttt acaaattcac aagcagccac
      841 acgttttcaa aaggaatcta gcgcacttgc tgatcatttg atcgaacaag tggaacagtt
      901 tctaggctat tcattaaaag aagatcagct actaaaacaa aatttaaggt ctcatttatc
      961 ttcaacttat tttcgactcc attatggttt gcagatttct aatccattga cgaaaaatgt
     1021 gtttagtacg tacacacaac ttttcttagt tttacagtta attttagaag attattttga
     1081 aaaggaaaat ttttatgttc cacaagagga gacagcttat ttaacgatcc atttccaagc
     1141 agcgatcgag cgacacaaac atcagaaaag tagaagatat caaacggttt tagttagtga
     1201 atattcaaaa gctatggcta cttttctcga agcacggctg aacagagaac ttccagaatt
     1261 aacgattatg gatctagttg aatataagcc agaattaaag agacaagatt ttccctctgt
     1321 tgattttatc ttggctacgt taccatttaa acatataagc attccagtaa tcgagatctc
     1381 accaatgatc acagaaactg atctagccta tttgacaaaa tatatgctgg agcatgtgcc
     1441 tatcaagaag aaaaaaacat ttgatctagc aagctttact catccatttc tgatctttcc
     1501 tcaacttgaa cggacagacc cagccgatat tttaaacttt atgggaaacg tcttagtgga
     1561 acatcattat gtggagccgg aattcatcga ttctgtcttg gaaagagaca gtcactccag
     1621 tactagagta gcaccatttg taacaatccc tcacggtaat ccgctgtatg tgaagcattc
     1681 gatgatttct attgctacaa tgaaagaacc agtcctatgg cacggggaac agatacgtat
     1741 cgtactgcta ttagcaataa agaaaaatga tttgaaacat gcagagttca aaaaaatatt
     1801 ttctgttatt cactatctag aaaagcagcc agaacaaatg gctcagttga tgcaatccaa
     1861 tcatccgcta gaaatcttaa cgttgttatc tagttatgaa taagtagcga ttaaaaaact
     1921 gcctttatat ttataataa
//
LOCUS       DANKHK010000177         1914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000177 DANKHK010000000
VERSION     DANKHK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..733
                     /locus_tag="PW109_002545"
     CDS             <1..733
                     /locus_tag="PW109_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2512312.1"
                     /translation="VDYYGIPMIFVNYAVFWGLFTGILMGDIKTGLIVGGTVQLMSLG
                     VAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVGMLGVQLDVIVKILNGFVVR
                     KSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTLGQSAVNYVLDVLPNWFMDG
                     LTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFLKVPIIGAAIIGFALAFNVY
                     HRAEKDAQITASAGASDVGFIGEDE"
     gene            726..1532
                     /locus_tag="PW109_002546"
     CDS             726..1532
                     /locus_tag="PW109_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2512313.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            1551..>1914
                     /locus_tag="PW109_002547"
     CDS             1551..>1914
                     /locus_tag="PW109_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2512314.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNN"
BASE COUNT          577 a          268 c          427 g          642 t
ORIGIN      
        1 tgttgattat tatgggattc cgatgatctt tgtaaattac gcagtttttt ggggattatt
       61 cacaggaatt ttgatgggtg atataaaaac aggtttgata gttggcggta cagttcaatt
      121 gatgtcatta ggagttgccg gttttggtgg ctcatctgta ccggactatg gaacgtgcgc
      181 aattattgca acggcatttg gtagccagct tggagtagaa gccggattag caataggact
      241 acctgtaggt atgttagggg tgcaactaga tgtaattgtc aaaatcttaa atggttttgt
      301 tgttcgcaaa tcacaggaat atgcgaataa acgagaattt aagaaaatga atagtgtttt
      361 gtggttgggg ccaattttct ttggtttatc tgcagcaatt ccagtattta ttgccgttac
      421 tttaggacag tctgctgtta attacgtttt ggatgtttta cctaattggt ttatggatgg
      481 gctaacatta gccggaaaaa tgttgcctgc tgttggtatt gcgatgttgt tacgctttat
      541 gcctacaggt cgatacatta gttatttact tgttggattt ttcttagccg cttttttgaa
      601 ggtacctatt attggagcgg cgatcatagg attcgcttta gcatttaatg tgtatcatag
      661 agctgaaaaa gatgcacaaa tcactgcttc tgcgggagct tctgatgttg ggtttattgg
      721 ggaggatgaa taaaatgagc gaaaaatatc aagtaacgaa aaaggatctt aggaaagcga
      781 actatcgttg gttgatgtca gtttgtacct ttaattatca aacacagcaa ggtgcatctg
      841 tgacttacgc attgtcacct attttaagaa aattatacaa agatgatgat gaatacatcg
      901 aagctttaaa taatcacttt cagtattaca atacacagcc ttggctggca gccattattt
      961 taggtgcttg tgttgcaatg gaggaaaagc aaggattaga agcaaaagat gcgattaatg
     1021 actttaaagt tggtacgatg ggtccaattg ctggtattgg ggactctctt ttgatgacga
     1081 tgatcccaac catcatggga tcaattgcgg cttatatggc tttagaaaat aatcctgtag
     1141 gaatcttttt atggttcatt ttaatcatgg taatcttctt tttccgaatg agaggctttg
     1201 aatttggtta taagcaggga atgaagattg ttactgaata cggtgaaaag attaattatt
     1261 taactgaagc tgcttcagta ttggggctaa cagtagttgg atcattaatt gcttcagtta
     1321 tcagtgtttc aacgccactt aaattcactt ttggtgaagt tagtatgaaa atccaaccaa
     1381 tgttggacaa aattttacca acattaattc cagtaattat tactgctgca gcatattatc
     1441 tactcagtaa gaaaaaaatc agtatgacca ttttgattct actcgttatt gtttttgcga
     1501 tggttatggc tgcatttgga atattagcgt aaaggaaaag gatttgaatt atgagaagat
     1561 tgatcattgc tagccattca atactagctg agggttttaa agagacacta gagtttttaa
     1621 caggtatgca taatcaaatt agtagcttat gtgcatatgt cgaagaaatt gatgatatag
     1681 atgaacaaat taaagagtta gtggatacta cagacgaaat aattgtttta actgacatgg
     1741 ctggaggaag tgtaaatcaa aagtttttta cctatatttc tgacagagtg cacgtaattg
     1801 caggaatgaa tcttcccttg gcaatgtcaa tcgctttgca gttaggaaat gataatttag
     1861 atattggagc aattattgcg gaggcacaac aacaaatcat ctatatgaat aatc
//
LOCUS       DANKHK010000178         1890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000178 DANKHK010000000
VERSION     DANKHK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(635..925)
                     /locus_tag="PW109_002548"
     CDS             complement(635..925)
                     /locus_tag="PW109_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512315.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1133..1825)
                     /locus_tag="PW109_002549"
     CDS             complement(1133..1825)
                     /locus_tag="PW109_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2512316.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          513 a          364 c          292 g          721 t
ORIGIN      
        1 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
       61 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      121 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      181 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      241 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      301 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      361 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      421 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      481 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      541 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
      601 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
      661 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
      721 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
      781 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
      841 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
      901 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
      961 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1021 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1081 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1141 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1201 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1261 atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg
     1321 cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt
     1381 cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga
     1441 tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga
     1501 ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt
     1561 catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt
     1621 tttgtgctag tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat
     1681 caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa
     1741 tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc
     1801 caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct
     1861 aaaagtaagt gatctcaaac acaatgtcaa
//
LOCUS       DANKHK010000179         1886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000179 DANKHK010000000
VERSION     DANKHK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            51..>1886
                     /locus_tag="PW109_002550"
     CDS             51..>1886
                     /locus_tag="PW109_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2512317.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNE"
BASE COUNT          739 a          257 c          331 g          559 t
ORIGIN      
        1 aaatagagat aataattgta cacttaagct gtattcagga ggtgaagata atgggcatcg
       61 aatttagcga acggcaaatg aaaatactat actttttgat tgaaaatgag cacacaactt
      121 acaaagaatt atcagaacaa ttatatgtat ctacgaaaac aattagcaaa gatattttaa
      181 ttttgaaaga aataatccaa aaaatgccag atgtacaaat gttggttcaa cctaaaaaag
      241 gcgtttcgct tattggaaaa aaagaaacac ttcgaaattt attatccagt cataaaatca
      301 atatcgcaga ctctccggag gaccgtctaa cttatgtgtt tgctaaatta ttgaatacta
      361 atgattctct aaagattcaa aatcttgctg acgaattata cgtgagcagg tcgacggtag
      421 aaagcacaat gaaagatgtt agaaaaagac taaacaagca tgggatgtcc ttagtttcag
      481 atcgaaatgg cgtgcgcatc aaagctgatg aaaaaagtaa aagaaggttg atgtcagaag
      541 ttgtgaacta ttattggggt ggctttacag catctaacag taaaacagat ctcttagact
      601 tgaatatcaa tttcattggt gaagcgaata atttcttaaa taaggacata ttgaaaaaag
      661 tagccgaatt gcttaatcga tacatgaata agacacaatt gaaatttaca gattatgaat
      721 ttcaaacttt agctatccac cttgctattg cgatagaaag gattgaaaca agcaatttct
      781 tatcacgtag taataattct gaagaagaaa tctcaaagaa tacacttatt ttaatcaaaa
      841 tgattgaaaa agcattttca attattgtgc caatagatga acaagcttat gttgacattc
      901 acattacttc gattgaaaaa aatgctatga atcacgactt agatcaaacg aagctttcct
      961 atttagatat gggtgttgag ctacaagagt tagtaagaat taaattaaat gaattttatc
     1021 ctgatgaaga attgatcaat aatctagtat tacatctcaa tgcagcagtt aaaagactca
     1081 aattgggagt aaatatctat aacccttata cggacaaaat caaatattct tttaaaaggt
     1141 catttatgat aagtgtagat ttattagagg aaatcgaaga gagattctgt atacatttta
     1201 atgaagatga aatcgcttac gtaacactcc atattcaatc acttttggat cgttacaaac
     1261 ctgacaaaac gaaagtgata ttagtttgta gtagcggata tggaacatca aaattattag
     1321 agcaaagaat aaataacggt tttgcagata tggttgagat agaagatgta ctaagcatca
     1381 atgaactaca agatagaaaa gtcactgatg aacttgttat aagcacgatt ccgattgaaa
     1441 atacaaattt tcctgttatt gttgttagtc ctttgatgtt aggggcagat gtcagaaaag
     1501 taaaacagta tctacagtta aatgaacaaa cagaagatgc ttttctaaat ttattagata
     1561 aacgattttg cttttttacc caccaaataa aggataatca agtgaacgtt ttaaatataa
     1621 ttaccaaaaa attggtaaaa gaaaattacg cgaaagaagg gatactagag ggagcaatca
     1681 aaagagaaaa actatcttca acagcgatga atgattttgc tatgccgcat atcgaagtag
     1741 attttataaa aaagccagta atctgtgtct acgttaatcc agaaggaatt gattgggatg
     1801 ggacacttgt tcattctgtt ttcttttttg cacttaatga aaaagtaaaa cctgaattga
     1861 ataaaattta tgaaacattc aatgaa
//
LOCUS       DANKHK010000180         1819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000180 DANKHK010000000
VERSION     DANKHK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            60..917
                     /locus_tag="PW109_002551"
     CDS             60..917
                     /locus_tag="PW109_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2512318.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            918..1466
                     /locus_tag="PW109_002552"
     CDS             918..1466
                     /locus_tag="PW109_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512319.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1733..>1819
                     /locus_tag="PW109_002553"
     CDS             1733..>1819
                     /locus_tag="PW109_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2512320.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYR"
BASE COUNT          689 a          251 c          380 g          499 t
ORIGIN      
        1 aaaataaata tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg
       61 tggtagcgaa gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg
      121 cgcaaatgtt agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact
      181 tggatatgca aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg
      241 aagaaggagg cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga
      301 taaacaatct tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc
      361 cagcagacat gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat
      421 tatcccaatt caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca
      481 tctatttaga tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg
      541 actgggcaat cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc
      601 ttgagtatat ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta
      661 ttcctttcat gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag
      721 agatttatgg ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgct
      781 acgacggatc aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc
      841 gcaaaagcca cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt
      901 atgcggaggt ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag
      961 gactcgccaa aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat
     1021 ctgaaaataa gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg
     1081 aaagacagag tagaggaaga acaaaaaaag ttggcgcacc aatcagaaag tttgataagg
     1141 tttattccac caagcatcct ttaaaactgt cgactttgtt aaatgcgact tccagagttt
     1201 tagtagaaaa gtatgagact aatatgacaa gagatgaatt gcttagaaaa gctttagatg
     1261 agtatattaa acagaatctt acgcaagaag ataagcaaga tttgtttaat gacgtggtga
     1321 aagaactaga cttgttcaga gaaaaatatc caattgttcc agaattagat gatcgtggag
     1381 aaataataag aactaccagt cagataaaac aagataccga agactcactg cgcaaagggt
     1441 ggggaatgaa taataaagtt agataataat caaagaagaa ctaagtaact acctttaaac
     1501 ggtagttact tagttcttct ttgatttgcg tgaaatagag gggatttatt tttctggtta
     1561 cgtgattaaa aaatccaaaa aatgagcata ggaacggtta tgattttagg gtgtgtttta
     1621 atgagatatt gtgatttcat taacaagtgt atgataatcg gttctgttgc aaagttttcc
     1681 aaaaaatcta ttttagtgta aaattgagaa aaaaaacaga gaggacagag taatgaatca
     1741 ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg
     1801 ttacaatcta agctatcgt
//
LOCUS       DANKHK010000181         1804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000181 DANKHK010000000
VERSION     DANKHK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            206..802
                     /locus_tag="PW109_002554"
     CDS             206..802
                     /locus_tag="PW109_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2512321.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(860..1342)
                     /locus_tag="PW109_002555"
     CDS             complement(860..1342)
                     /locus_tag="PW109_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512322.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1448..1645)
                     /locus_tag="PW109_002556"
     CDS             complement(1448..1645)
                     /locus_tag="PW109_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512323.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          610 a          363 c          275 g          556 t
ORIGIN      
        1 aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa atccttacat
       61 aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg agcatatctg
      121 tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa
      181 cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga aaaaccgccg
      241 ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa caactttgat
      301 caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac gcgcagttat
      361 ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac ttcgcccatt
      421 gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca aaaatggtta
      481 tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag aacaatttga
      541 attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg caacagctaa
      601 tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt acaacccagt
      661 tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac tacgttcgca
      721 attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga acgatgccga
      781 tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg tgacaaacat
      841 cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct gcctcatcgt
      901 ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat ttcagcatcg
      961 gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct tttggctgat
     1021 ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa
     1081 caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa atcattgctg
     1141 acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta taccaaatga
     1201 ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca gtccgataaa
     1261 taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat aaaaacaata
     1321 gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca gaaaatagga
     1381 aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt
     1441 gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt tcatctaata
     1501 attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt gtcatttttg
     1561 atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca tattgttctc
     1621 ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac
     1681 attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca gttaaaaatc
     1741 aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag ctgttgtatt
     1801 taga
//
LOCUS       DANKHK010000182         1673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000182 DANKHK010000000
VERSION     DANKHK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            6..842
                     /locus_tag="PW109_002557"
     CDS             6..842
                     /locus_tag="PW109_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2512324.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            1090..>1673
                     /locus_tag="PW109_002558"
     CDS             1090..>1673
                     /locus_tag="PW109_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2512325.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKY"
BASE COUNT          583 a          251 c          324 g          515 t
ORIGIN      
        1 aaaatatgct gcggtttttg attttatctg ggtatttcga attgatgatg tacttgcaag
       61 tttctggcaa actcgatcaa tatatcaatg tccattatcg ctacttagct attctttcaa
      121 tgattttatc tgctcttttg gctttagttc agctgtattt gtgggtcaaa aacggaaaag
      181 cagaagaaaa ggcacacgag cacgatcatg accatgggtt gtcaaaacct tatcaacgtg
      241 gaatcgctta tgtactgcta gctttgccag taatcgtggg gacgttgttt ccaacagtta
      301 gtctcgacac tacgatcgta gaagccaaag gatttaattt cccagtaagt aaagaatcag
      361 taggggatcc ggagatgcag acacaatact taaagccgga taccagtatt tatttcaaca
      421 aatcagacta cttagaccaa atgaatgagt tgaaagaaaa gtatggggat aagcaaacta
      481 tcaagatcac agatgaaaac tatttagaag ttatggagct aatttataat taccctagcg
      541 aattcattgg caaaaaaatc agctatgaag gatttgttta ccaaacaccg aacgatagca
      601 atacagatat cttcttgttc cgattcggca ttatccattg tgtggcagat tcaggagtct
      661 ttggtttgtt gacgcatatg ccggaaggag taacggttaa aaatgatgaa tggtataaaa
      721 tagaaggaac catccaatca gactattatc aaccattcaa gcgtgaaatc ccatctgtag
      781 ttgttacaaa cgctgagaaa gtctctgctc cgaaaaatca gtatgtgtat cgttcttttt
      841 aaaagaacaa aataaataaa catagctcag acagctgaca cgcaaggacg tcggttgtct
      901 ggcttttttt gcatgatgta ttcatttgtt tgatatttat tttagaagca gcatcctgaa
      961 agtatttgat atcggtttca aaggtgttgg aaaagattgt gaagagaatt acaatttatt
     1021 cctaacatgt atgttgaaaa gttgtatgaa atgattttga tagtaaaata tcgagtggag
     1081 gtacttttta tggtaacgcg tatatttatt ttggatgata gtttggtttt tgaaaaaatg
     1141 ctagtagaaa ttttggaaga atccagaatt ttattaagtc cttggaaatt tgagatcgtt
     1201 cagggattga acgttataca atttgttgaa ttcattaaaa ataatgaaat ctattcctca
     1261 gatatttttt tcctagacat cgatttgaac gcctattata gcggtatcga actaggcgaa
     1321 atgcttagag aaaagaatga aaagtgtaaa attgtttatc taacgtctca tccgaataaa
     1381 gcaataactg ttattaatcg aaagattacg cctagtggtt atttgataaa agaattcact
     1441 tatgaaaaaa ataaaaaaag catccaaagt tacttattga aagaaaaaga aaactatcct
     1501 gttcaacatg ctacgaaaaa aaggtggaca actgtccgtt atggtaatag cgatcactat
     1561 atccattacg atgaaatcct ttatctaatg agcatgccag gttataaaaa taaaataggc
     1621 ctagtattga aagatgaaga gattctcgta aacggcacaa ttaaaaaata taa
//
LOCUS       DANKHK010000183         1669 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000183 DANKHK010000000
VERSION     DANKHK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            251..862
                     /locus_tag="PW109_002559"
     CDS             251..862
                     /locus_tag="PW109_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2512326.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            1044..1559
                     /locus_tag="PW109_002560"
     CDS             1044..1559
                     /locus_tag="PW109_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HDL2512327.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT          547 a          213 c          328 g          581 t
ORIGIN      
        1 attttttata gaaaagtttg caaagaacta ttggatggaa aaaacatata tgatgtcaga
       61 ggaatacttt tgatttctgc aatcacgata gctggtggca tcctaatttc aataattaat
      121 aaatttgatt tcataaaata tttaattata gcaattgctt gtgtcgctgc aattatattt
      181 agaaaacgat tgctactttt agtaaggaga attatgagca aataaacatt tgctcataga
      241 gagatagaga atgatagata ttatttctgc tataacggta aatgtattaa cagcattata
      301 tcagccgttc ttatttgccg tattaacagc gtatctgttt atgtactggt atctatttat
      361 agttgataac tctcgagatc ctaaaaactt aaaagattct gtattaatct ggttgagata
      421 ttttaaagca tctagtaaat ttcgcagact cttttttctt gcgttttatt ttactttgat
      481 attatttaaa accttgctta atcgaaatat gtggatgaac ccgctttcaa acgttattgg
      541 acagtggtca ttatatatta tagataatgc tacaggagag agcagattga caacagaatg
      601 cgtggagaac acgatgctgt ttatcccttt ttgcatgtta ttgttatggt ggcgtgacat
      661 taaatgtgat attatacgca ccatctatgt tgggattaag tatgtgtttt tattctcatt
      721 aagtatagaa tttacacaac tgttttttag gcttggtaca tttcaactat cggatttgtt
      781 ttataacacc ctagggggct taattggtgc tttgctatat tggctattct accgattaaa
      841 taagtatata gacttaaaat agaactgctt tgaattttaa tatggttaat atttttctgc
      901 agacaggatt gtgtaatctc atcaagaata ctttacatgc catctatttt tgctaaaaca
      961 ttgttgttat atcttcaaga ggtagggaat tttattgctt taaatgaggt cgagcgaatt
     1021 aaaaaattgg taaaatatat aacatggtgg tagttatggt aaaagtgttt gatataatta
     1081 agttttcgga tttcagagga tatactgctg ttgcattgga taatgagact cttttctcga
     1141 ttggtattga gtttcacatt aagcaaataa atcaaggatt tagtcataat aaatatacta
     1201 tgcgagggct acattatcag agtggagagc atgctcaagg aaagatagtg atgtgcctac
     1261 atggtgaaat tatgaatatc gcacttgata ttcgacaagg atcttccact tatggatgtg
     1321 tgtatgtcaa cacattgagt gaaagcaatg gtaaatgtgc gtatatccca cgtggatatg
     1381 ctcatggcta tcttactatg gaggacaata cgctagttca gtggtgcgta gataatgatt
     1441 tcaattctaa tgctgccaaa tgtataagtt atgaaagcgt agatattgaa tggccggtca
     1501 gtcggcagtg tttcgttata agtgagaaag acaaaaatgt ggaaagatta agcttttgat
     1561 taaaatgagc tgtggagaaa agaaggatac tggaaaataa gaagaacaat aaccgagcaa
     1621 atgctcggtt atttctatga ggaatacata aaactaaatt tagatatag
//
LOCUS       DANKHK010000184         1627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000184 DANKHK010000000
VERSION     DANKHK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            100..393
                     /locus_tag="PW109_002561"
     CDS             100..393
                     /locus_tag="PW109_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512328.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            386..682
                     /locus_tag="PW109_002562"
     CDS             386..682
                     /locus_tag="PW109_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512329.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            753..>1627
                     /locus_tag="PW109_002563"
     CDS             753..>1627
                     /locus_tag="PW109_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2512330.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSY"
BASE COUNT          627 a          225 c          322 g          453 t
ORIGIN      
        1 gactttaaaa atacatgtga tgatacatgt tatatgaatt atcaatgtac ccagtaatat
       61 gctattttca ttatgtaaaa aataacaggg ggtgtgtaga tggagattga tgaaacttgt
      121 ttgagtaact cttataaaag tatgaatgac ttttttgagg aaatggaact ggattcagaa
      181 ttgctctatc aattttatct tgcttttcga gggcaacaga tttcttttcc aatgaaaatg
      241 tacgatcgtg agttagttag aaaaagaatc gaaaacatga tagaacagga aaaaactgta
      301 gatatccgtc aattgacaga agtttatggt ttctctgcaa gatggattca aaaagtaatt
      361 aaacaaaaag aaaggagacg agtacatggc taagctgatt gtattaggtg gtttaattta
      421 cggtggatat tggttatata aatatatgct tgcgcatccg aatatagata aaggaaaagt
      481 aattaagaca gttggggtaa tcatagttgg actcatagct ctaactaaga aaaataatac
      541 taatagccca aaaataatga gaacttatcg ctgtagagga tgtggaaggg aaatttctac
      601 tagttttaat ccatctgaaa gccctcaagg aagagatcgt tgtcatgctg gctatcgaca
      661 ttcttgggaa aggattaact aaatgaacac cattgagatt aattgactga taaaataagt
      721 acgcgaggga gaaattgaaa gggggaaata aaatgaaaaa atggttgtta ttactaattg
      781 gaatggggat tttattcact ggttgtacat ctgagaatac gaggactaaa gagactgtag
      841 aaactgatac aacaattgag gctaaaaaaa gtaaaacgca atcaactgaa caaactacaa
      901 cagaaatgaa acaatctgtg gaagcaagta caagtgaaag ttcaatcatt gaatcttctt
      961 ctgaagaggt taaaaatact ttgtgggata gtgagaaatc tactcaacta gcaatttttg
     1021 tcccacaatg gggaaagaca ttaggtcaag aatataaaag ctacaatcct cagaacaatg
     1081 taagtctgta tggtacgcct ttaccaagtg cagttattga tggaaattgg aaaatggcaa
     1141 tcaatgaagc accagttacc gtacaatggt cagaagatgg aactggtcat gcagatttca
     1201 atttagtagc agtttattct gatgtagaaa ctggagaata tttagggcag catgtatatt
     1261 tctttggctt tcaaaatgga caacctaaag tttatttaac gcaacaaaat caaggcaatg
     1321 ataaaaacta catgtacttc aatgaaactc agaaccaaca gttaaaacaa ggtttcattg
     1381 atattgtgaa tggacaaact cctcaaactc cagtagtaga agaatctact caacaagcaa
     1441 caactacaac gacacaatct acaagtagct cagcaatttc aagttgggaa gctgctaaag
     1501 aaactgtact aaataataaa gctttatggt tgtatgattc tcctgataaa aatgcatcaa
     1561 atactaattt aattagcaca aatgaatata tgaaagatct aatgcaagat gaaaatggaa
     1621 gctacta
//
LOCUS       DANKHK010000185         1613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000185 DANKHK010000000
VERSION     DANKHK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            138..>1613
                     /locus_tag="PW109_002564"
     CDS             138..>1613
                     /locus_tag="PW109_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512331.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTVSLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSNKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          595 a          223 c          292 g          503 t
ORIGIN      
        1 tctcaaaaaa agataaaaaa tgagaataac tgatgaaaaa acaagcataa tccatcataa
       61 aatcagtata ggtgaaaaga tgaactttca tctgcatatc aagtaattgg tatgaggcat
      121 tttttgaggt gtaggatatg aagttggatt accttataga agaggaagat agagaaaaac
      181 tgctgctagc tagatattta gaatttgaga aaaagaatta tgtagtgctg cctaagttat
      241 atgaaacatt aggtttgtct aaatttaaaa caagaaatta tatcatagaa ttaaataaag
      301 aatttactga attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg
      361 tcgaacgtct gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa
      421 aaattgctcg actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag
      481 agaatcattt tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc
      541 ttgaacagca tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac
      601 ggaacgtatt atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag
      661 aaagcgtcat gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa
      721 aactgtctgc aacaaaacga atcaaattca atttgctaac cactgttagt ttgttgaggg
      781 cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag
      841 aaggaatgaa gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc
      901 agatcattga tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat
      961 tagatggcgc atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa
     1021 aaatgtcagt ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa
     1081 gactatatat acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga
     1141 tattcgactt tgagatgact agctttacgt ctaacaagca gctacagttt ttcttcgaga
     1201 cgtatccaat attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct
     1261 tgggaaaaga tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg
     1321 gtatttgtcc ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag
     1381 gatctgctta taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg
     1441 tgattgagag tcgaatcact tctcgtacga atatttattt atctgattgt ttacttgaaa
     1501 aatttaataa agaacagata atttggaaaa atccaccgac tccagatgat tgggaatatt
     1561 ttggaaatac catcatcaga gaaaaaaata gacatttaac tgaaaaaatc agc
//
LOCUS       DANKHK010000186         1562 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000186 DANKHK010000000
VERSION     DANKHK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            122..340
                     /locus_tag="PW109_002565"
                     /pseudo
     CDS             122..340
                     /locus_tag="PW109_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            600..854
                     /locus_tag="PW109_002566"
     CDS             600..854
                     /locus_tag="PW109_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2512332.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            855..1172
                     /locus_tag="PW109_002567"
     CDS             855..1172
                     /locus_tag="PW109_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2512333.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          545 a          238 c          301 g          478 t
ORIGIN      
        1 tcgatcagtt ttgaggaaat gaaattctag atattttttg ctacatagag ttactttcaa
       61 tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg atgaaattgc
      121 tattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt ggtctaatgg
      181 atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa ataaagttat
      241 tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga acactcgcaa
      301 ctttattcgt gtagatgaat cggcttcggt tgagttataa ttttagctat atcaatcaac
      361 cgaccgacat atgtcaaatc tatatataat aaaaatacct acttaaatgg atgttaacgg
      421 aagtgtatgg tgagcagttc actcgtattt tgatatcgtt tgtatatgtt ttcagcgttc
      481 taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa acaccgttgg
      541 caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag tgtgataaca
      601 tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc cttccaagct
      661 ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac ttattcatat
      721 tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa agtttccaag
      781 actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc ggaaaatacg
      841 gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc cagaccagcc
      901 ttgaaacgat aatagaagaa tgggaaaatg agcttgctct ttctccagcc agcataaaaa
      961 gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca actatgcatg
     1021 aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg atcggcaaaa
     1081 gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta ggacgtattt
     1141 tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt cgctcatcct
     1201 ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta ctcgaagatt
     1261 gatatataag acttagtacc tttttatttt actccatgtt atggccgaag ctctaaagat
     1321 acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc gcttggtgaa
     1381 taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt cccaaaaatt
     1441 tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt gacgtaactt
     1501 ttaataaata ctacctaaat aaggtagtat ttatttttgt gttttaaaag aggaagtcca
     1561 at
//
LOCUS       DANKHK010000187         1561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000187 DANKHK010000000
VERSION     DANKHK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..479
                     /locus_tag="PW109_002568"
     CDS             <1..479
                     /locus_tag="PW109_002568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512334.1"
                     /translation="TRYILKQKTLGLFFLALTPVIDLILLITTGIDLMNGATAATPHA
                     IAAVYISVSLVFGKSMINWADDRFGYYVMKQGPKPYKPVGLAYSKNYAKSWLKHLLSY
                     IIGTGILHLIIFLINDKSRTEAMDNVIHVWTIVIIIDLIICISYFVWPPKNTESKL"
     gene            616..873
                     /locus_tag="PW109_002569"
     CDS             616..873
                     /locus_tag="PW109_002569"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2512335.1"
                     /translation="MTKYSESFKEMIVDLANNGTKAKDLSSEYGISEAAIYKWKKLYT
                     KDSKTGVNEKQMQTLRKEIKQLKLENEILRKAAAIFAQDPL"
     gene            852..1169
                     /locus_tag="PW109_002570"
                     /pseudo
     CDS             852..1169
                     /locus_tag="PW109_002570"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          590 a          232 c          265 g          474 t
ORIGIN      
        1 taacaagata tatcttaaaa caaaaaacac taggtctatt tttcctagca cttacaccag
       61 ttatagattt aatattactt ataacaacag gtatagacct catgaatgga gcaacagctg
      121 caacccctca tgctatagct gcggtctata tttcagtatc tcttgtcttt gggaaaagca
      181 tgataaattg ggcagatgat agatttggat attatgtgat gaaacaagga cccaaacctt
      241 acaaacctgt aggattagct tactctaaaa actatgctaa aagttggttg aaacatttac
      301 tttcttacat tataggaaca ggtattttgc atttgattat ttttttaatt aatgataaaa
      361 gtcgtacaga agctatggat aatgttattc atgtttggac aattgtcatt attattgact
      421 taatcatttg tatttcatat tttgtgtggc caccaaaaaa tacggaaagt aaattatgat
      481 tacaatttta aaaaaagaaa atactgacgt tttgaactga cctcaattag tgggagttgg
      541 tgctagtatg aaaattagga cgccttttcg tagagtcttg aatcaaatat aatgagacta
      601 aggaaggtgt ctaaaatgac aaagtacagt gaatctttta aagaaatgat tgttgattta
      661 gctaataatg ggacaaaagc gaaggattta agtagcgagt atggcatttc agaagcagcc
      721 atttataaat ggaaaaaatt atacacgaaa gactctaaaa ccggtgttaa tgaaaaacaa
      781 atgcaaaccc ttagaaaaga aataaaacaa cttaaactcg aaaatgaaat actaagaaag
      841 gcagccgcca tattcgctca agatccactt taactaaagt ccgttggatt aaacgctatt
      901 caaaaatctt taatgccagc catctatgcc gcatattaaa tgttccaaga agcttatatt
      961 attactgtat taaagagaaa tattctgtta gaaatgagag actccttcgt tttaaagttt
     1021 taatcaaagc cttataccat caatataatg gcatctatgg tgcgcctaaa atccacgctt
     1081 ttttgacaca aaaaggctat aatgtttctg ttaagtatgt tcaaaaaatc atgcggttac
     1141 ttggcattac ttctgttacc attaaaaaat tcaaacacta tactaattcc aaagttaaag
     1201 gtgtctttaa aaatatgctg aaaagtatga tgctatctat cttaaagaat gcaaccatcc
     1261 taatgaaatt tggcaagcag atcatactat gttagatata gaggttttaa acgaaaaaaa
     1321 caaacttgaa agaccttggc taactgttgt cttagatgat ttcagaacaa gaaagaaatg
     1381 agagtaaacg tcggcaaacg caagggatta aagtagcgaa agaaaaagga gtatacaaag
     1441 gacgacctgt actttattca ccaaatgcaa aagattctca aaaacgttta gtttattact
     1501 gagttataga aatgttagac aaatgttttt caattagcaa tatagttacg gaagtagatg
     1561 t
//
LOCUS       DANKHK010000188         1498 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000188 DANKHK010000000
VERSION     DANKHK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            121..1416
                     /locus_tag="PW109_002571"
     CDS             121..1416
                     /locus_tag="PW109_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2512336.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          472 a          295 c          275 g          456 t
ORIGIN      
        1 ctctatactt tttgatgttg gtgaacttca ccaacatctt ttttgtataa aaaaatatag
       61 aaaagacacc ctgaaatcct ttagaattaa tttggctacc aattcatagg aggattcaag
      121 atgtctcata ctcagcttat caaagatacc ctaaatattc tagacctaaa tatctatttc
      181 gaggaaaatt gtttaactaa agaaaaatac aaagggcaaa tttgcttgat ttacaaagga
      241 actcttctgt acaagccaga agagtgttcc cattgtcttt gtgtcgtccc ctctaggatc
      301 attcgttggg ggacgacaac cgttcggttg ttactgaatg atgtgtctga gtatcgcact
      361 tatctggagc tgaagaaaca acgatttaaa tgtaagtctt gccaaagaac attcgtagcg
      421 gatacctctg ttgctgaaaa acactgtttc attagtcaaa aagttcgttg gtctgtcatt
      481 gctcgcttaa aagagaatac ttccatgacg gaaatcgctc gtcagaaaaa tatttccact
      541 tcttcggtct atcgtgtcat gaagcgattc taccgcccat tgaatccttt caaacagact
      601 ttgccaaagg tcttatgctt tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg
      661 agttttatca tgatggacgg acaaagccat gcattattgg atattgtcga aaatcgacgc
      721 ttgccttatt tagaacgcta tttttctcgg ttttcattag cgactcgtga agccgttcaa
      781 ttgattgtta ttgatatgta tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat
      841 gctcagttga tcatcgatcg tttccacatc gtccaacata ttggcagaac tttcttgaat
      901 catcgagtga aagaaaccac ggtacgactt aaaggaaatt cccactctca acgagggtta
      961 gggaaaaaat tgaagcgcta ttggaaactc ctacaaaaag aagaagcaaa gttaaactat
     1021 gaaaaacggg tttggcgtgc cagttttaaa gattatttga ctgaaagtga gattgccgat
     1081 cgactgttag ctgcttgtcc caacttacgc caaggctatc agttatacca agacattctt
     1141 tatgcggtaa agaaaagaga tcagtcacta tttgaggatt gtttaactag agacattagc
     1201 ggtctgccag aaacctatac aaccacttta cgaacgttta aaaagtttct gccacagatc
     1261 caaaatgcgc ttcactacag ctattcaaac ggaccattgg aatgcttaaa caaccacatc
     1321 aaagtattaa aacgcaatgc gtacggcttt cgcaactttt acaatttcaa actacgaatt
     1381 atgattcgtc atggcaaaac atttttaacc aaataaacag aacagaaaga ggttttcttc
     1441 tttctgttct gtccaaatta ttctaagatt tctctttacc aacatcattt gacaaaga
//
LOCUS       DANKHK010000189         1419 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000189 DANKHK010000000
VERSION     DANKHK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..1382)
                     /locus_tag="PW109_002572"
     CDS             complement(<1..1382)
                     /locus_tag="PW109_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2512337.1"
                     /translation="MLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTN
                     DLECITDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFFNVLGHYLSGEH
                     NWSNIADTTFISLSKVYNLRNELINFFKQMQYLNADGHFEIPEKDYRSLLLTIIYETN
                     RTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFS
                     QEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKDL
                     NVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKT
                     YLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRKLTISLSLLNEHKRKSPIEVF
                     IVAPSDFRYLYYRQQLEDILGEHFSISNIICKQLREIVDDTFFCTQRIILCDSSLYQE
                     GLGSEKTIIYP"
BASE COUNT          462 a          239 c          242 g          476 t
ORIGIN      
        1 ggatatataa ttgttttctc actacctaag ccttcttggt ataaagaact atcgcaaagt
       61 ataatccgct gagtacaaaa gaaagtgtcg tctactattt ctctaagttg tttacaaatg
      121 atattactaa tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg atagtataaa
      181 tacctaaagt ctgacggagc tactatgaac acttcaattg gagatttccg tttatgctca
      241 tttaaaagac ttaaactaat ggttagttta cgaatagaat tctcattcca acgcagaaat
      301 aaattgtttt gttttcgcca ttgttcaaga atttcatgaa cttctaagta caaagcgcgt
      361 tgctcttcat ttaacaagta agtcttatca ggttgaaata tctgaatatc agcccaaagt
      421 gagcggataa acgggagaag tgctttatca aaaagtatct gattttcttc agatatattc
      481 agagataaga taagtttttg actgagctca atcaccaaag ggttatctgc tacatggttt
      541 tgataaacaa cgttcaagtc tttttgtaac aattcaaagt tatcgcatag ataatttcta
      601 gagttgaata gaaagtagat ataaaggcat tcatcttcgt catgacataa agagaaagaa
      661 agttgctcta atcctttctg tattagctga aacaagggag tatgtcgggc aactcttttt
      721 tcttcctgac taaaatatat tgggttagtc ttagatctct gcaaaccgat agcgatacct
      781 aataaaataa attgtttttc aactgtaggg taacttcttg aaaaaaaatg tttttctaca
      841 taatcaatca attgttgacc gctttgaata attgatttat ttagctggat caactctgtc
      901 cgatttgtct catagattat cgttagtaac aaacttcgat aatccttttc aggaatttca
      961 aaatggccat cagcatttaa gtactgcatt tgtttaaaaa aattaatcaa ctcatttcgt
     1021 aagttgtaaa ctttgcttaa cgaaatgaag gtagtatcag ctatattact ccaattgtgt
     1081 tcccctgata aataatgtcc tagtacattg aaaaagtcag agcgctgata gatgagttga
     1141 gtgagtgcca acctcgaaaa cgaagagtcg aatgcgactg taaagctact tctagatttc
     1201 tccactgaaa taatttgctg atggagtatg tcggtaatgc attccaagtc gttagtgaca
     1261 gtgagtgggc aaacgttcaa ttgatctgct atttcgcgaa tctgaatggt ccctttttcg
     1321 tgtaattgct cacaaatgta aacttgacga aaaatacttt tctccagata ggtttccaac
     1381 atagtactcc tcctagtcat tttatacata actattata
//
LOCUS       DANKHK010000190         1389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000190 DANKHK010000000
VERSION     DANKHK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(897..1310)
                     /locus_tag="PW109_002573"
     CDS             complement(897..1310)
                     /locus_tag="PW109_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2512338.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          509 a          235 c          203 g          442 t
ORIGIN      
        1 gagatctttt tatacttcaa aaaagataac acccaacttt caaaaatatg ttttggaagt
       61 tgggacaaac gagttaatac attttaatac attatttttt attcaaaaaa aatagcacaa
      121 tggcaaagat cgtaagagag atcataagcc ccaatgaatg actaaaaaga aaagcagaat
      181 cacatgccat atcatcccat atttcttcaa gaaaataaat aagcgggaaa aagggaagga
      241 atagtagaac atcgatccag caccattttt ttcttctttt gaaataacat aaaacaataa
      301 acatgaacgt gcagggaagt aaaaacaaag ctttactcat aactaacaaa ataccagctc
      361 cttttatttt tttctccccc acattcaaag agtaacaatg gtatgtactt aataatagaa
      421 aaatggatca acttgtatga ataacagctg aaaagatctg caccaaataa aaaacttcca
      481 gccaaaactg actggaagtt tatttttttc tgaaatattc taacacaaat atgaaaaaga
      541 tggccgaagc taataagccg atttcgtagc aaaacaatga gataccagag tcgtaataat
      601 aaaatcctct aatcttatgg ataaaataga tcatcggaca agcagataag agtaagtatt
      661 tgttaggcct tttgaataag taaaagaaag cactggtcat tatgataaat ggtatccaaa
      721 acaaaagttt acttaagaca atcaaatgat caactcccat attttttacc aagagagatc
      781 ataacatctt tatcgtaaaa acaaaatatt tttttgctca ttttgaaaat ttaaacatac
      841 tcgttcttat ccaaatatat aaaaatcgcc cctcattcat cgagaggcga taagttctaa
      901 tttaactcaa ttttttttgt ttcttttggc tgataagctg ttttaggtaa taccaatttc
      961 agcacaccgt ttttaaaggt tccagtgatt ttttcttcat caacattagg aagatagaat
     1021 gaacgtttgt aagagaccga atgtctttct ttccgtacat agttgccatc ttctttatct
     1081 tctgtatggg actcatgatt ggcggagatc gtcaaagtat tatctgaata ttcaactgtc
     1141 gtgtcttctt tgtcacagcc aggcatatca gcagttagtt tgtattcttt ttcattttcg
     1201 actaaatcta cttttggata acttgaaaca cctaaaaaat cattgaatgc tggactaaac
     1261 acgtcattga aatcagggaa catgtcgcga atgctaggta catttgccat ttttgatcat
     1321 cctttcaata agtatttttt atttatccct atttacgtaa taactctatt ttgaaaaaaa
     1381 tgcaagtat
//
LOCUS       DANKHK010000191         1355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000191 DANKHK010000000
VERSION     DANKHK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..1355
                     /locus_tag="PW109_002574"
     rRNA            <1..1355
                     /locus_tag="PW109_002574"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DANKHK010000192         1354 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000192 DANKHK010000000
VERSION     DANKHK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            82..>1354
                     /locus_tag="PW109_002575"
     CDS             82..>1354
                     /locus_tag="PW109_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL2512339.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          458 a          254 c          259 g          383 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DANKHK010000193         1351 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000193 DANKHK010000000
VERSION     DANKHK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            74..382
                     /locus_tag="PW109_002576"
     CDS             74..382
                     /locus_tag="PW109_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2512340.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            409..708
                     /locus_tag="PW109_002577"
     CDS             409..708
                     /locus_tag="PW109_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2512341.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            882..>1351
                     /locus_tag="PW109_002578"
     CDS             882..>1351
                     /locus_tag="PW109_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2512342.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEG"
BASE COUNT          494 a          196 c          287 g          374 t
ORIGIN      
        1 atattatatc catgaagccc aatcttcttt aatagaagat tgggctattt ttatggcaaa
       61 ggagggaaaa gaaatgtttc aaaataaagg acaacgttat gtcacaaagg gagtaatgga
      121 cagtttgccg gtggaattac aagctttatg ttggaatcta atcgatcaaa acgttcaaaa
      181 gcaatttcat ttagactatc ttcaaatctt tgaattttct acggaaaaag gaaatcaaaa
      241 gttggttcat cgtcaagaag aaccagaagc aagaaaagag tatctgattt cacctaagct
      301 tcgtttgaaa tcagtgtctc aaaaaatatg ggtcattgat tcgggtgagt atcaaacgat
      361 gttattacca gaagaatact gatatctaaa agaaaggaac gaaaggactt gttagaaacg
      421 gctatcgaag tactggaaaa gtgtgcacag ctagtcacta ctcctgaaga gtggggctat
      481 gaatccgtta cgatggaaaa agaggaaatt gaaatgggta tgcttcctga agatgttact
      541 ctaccacgtt tagtgatgac acatctttat atttattgcg caccagaaga tggaaaagac
      601 tatgtagttt atttcattac agatattacc agccagcgag aatttgttcg tgggttactg
      661 gtagaaggac gattggtttg gagtcagatt ggaggaacga atgaataaga tcgattattt
      721 gagaatgatt gcgaaatgtt tagaagatat ccttacagcg attcatcaaa ttctgaagaa
      781 aaaaggagaa agaatatgaa atattttcaa ctagtattga cacttataac ggcaatggtg
      841 attgaattgc ttgaacaaaa attagcatag gagaaaaaga gatgattgaa aaagaattag
      901 tgaccacaca aacagaagtt tggcgagatc ctcatcatac ccatcgctat gttttcaaac
      961 gacaatgggc gatcaaagga aaagaagcgg aagataaatt agcggtagtc attactattc
     1021 gtccaacaga tacagaacca tttacgaatg atttgagtat gttattaatc gaaaagaata
     1081 tccgacaatt aggatttact ggttttattg ctgtgaatct tttttcgtat acaaaagcga
     1141 aaagtcctcg ggtatttcct cgtggaaatg atgaacaatc tttggaaata ttaacaactg
     1201 ttttcacaga aaagaaaatc aacaccatta tttttgcttg tggttcgatt acagcgacga
     1261 gtcaagtggc ttatgaacaa gcgaagacca tttacgatca attgagtgcg aaacaaaaaa
     1321 agatgaccaa agtattgata aatgcagaag g
//
LOCUS       DANKHK010000194         1345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000194 DANKHK010000000
VERSION     DANKHK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..943)
                     /locus_tag="PW109_002579"
     CDS             complement(<1..943)
                     /locus_tag="PW109_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2512343.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFSID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDI"
     gene            1087..>1345
                     /locus_tag="PW109_002580"
     CDS             1087..>1345
                     /locus_tag="PW109_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512344.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHG"
BASE COUNT          406 a          226 c          261 g          452 t
ORIGIN      
        1 atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa tttaatgatt
       61 ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca tccgtgtccc
      121 taaagtccat tgttttaggt aagccatctc gtctaagcaa gccgtttgag tgctcattta
      181 gccctctttg cgaaggtgtt cctggatctg cgaaaaagat ggcaatatcg ttcgagttac
      241 ttatagatcg ccagttggaa aattcttttc cacaatcaaa tgtcatggat ttaaacaagt
      301 gtttaggcat ggatttgaac caatcattta acctgttttc gatatcttcg gctcgtctgc
      361 ctgtcggttt caaagtaata ataaccttgg ataaacactc aaccaatgta ataacagcgc
      421 tcttgtgttt aactcccaca atggtgtcga cttcgagatg accaaattct ttagtcaggt
      481 gtggatagag actgttccgt tcatgaatcg ttctgcgaaa ggcctgtttt ccacgctttt
      541 cttgatgatt attcggttta cgtttcccct tcataggtaa cgactgtgtg tcaaaaaggc
      601 cttttttaaa catccgatag agtgtacgaa cagaacaatc aattgaaaac tcgccacggc
      661 cgataatcac atcgggtgac catccttgag ctaccttttc ttgaacatat gttgattgtt
      721 catcagataa aacggttgaa cgtcgtccac aacgagattt attttgtttg tattgacggt
      781 aataatctag cacttttttt ccttgtttga gaaacgaata aacattataa atggtttgtc
      841 tagagcgatt taaactttgt gcgacaagag cgacaggttt ctcgatattg aaataagact
      901 ctatgattac tagttcgtct gttgtaagat gggtgtaagt catttgtact cactccttat
      961 taattctttg gtcggaacta ttttgagtgt agcacaaatg atctttttag ttgtctagct
     1021 taattttaca atcgacgttt taataaattg tgatataatg aagtataact ttttagggag
     1081 tgtactatga attttcaaga taatagacca ttatatttac aagtagaagc ttctatacgt
     1141 aacgatattc gtgaaaaaaa atatcttcca ggagagcaat tacctacaga agaagaatta
     1201 tgtaaaattt atggagtaag taagattacc attcgaaaag cttttaaact tttagcagaa
     1261 agtggaacag ttgaacgatt aagggggaaa gggacctttg taactcaaaa aaaagaaagt
     1321 ttaaaattag ctggagttca tggat
//
LOCUS       DANKHK010000195         1329 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000195 DANKHK010000000
VERSION     DANKHK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(54..1226)
                     /locus_tag="PW109_002581"
     CDS             complement(54..1226)
                     /locus_tag="PW109_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL2512345.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          362 a          275 c          252 g          440 t
ORIGIN      
        1 gaagtcaagt ccataatcct gtgtaaaata ctatacaatg ttttaccggt ctctcattga
       61 tcaaacttaa tatattttct tgtagaactg agccattcgt catgaaggtc cataaggaca
      121 gctccaatta atcgattggc agacgttcgg ttgggaaaaa tccgaataat cttttctctt
      181 ctgcggactt cctggttcag tcgttcaaga aggttggtgc tttttagccg attatgacta
      241 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
      301 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      361 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      421 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      481 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      541 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      601 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      661 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      721 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      781 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      841 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      901 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      961 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
     1021 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
     1081 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
     1141 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
     1201 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1261 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1321 tttatcaat
//
LOCUS       DANKHK010000196         1325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000196 DANKHK010000000
VERSION     DANKHK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            12..272
                     /locus_tag="PW109_002582"
     CDS             12..272
                     /locus_tag="PW109_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512346.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(634..873)
                     /locus_tag="PW109_002583"
     CDS             complement(634..873)
                     /locus_tag="PW109_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512347.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1209..>1325)
                     /locus_tag="PW109_002584"
     CDS             complement(1209..>1325)
                     /locus_tag="PW109_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2512348.1"
                     /translation="SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          456 a          187 c          251 g          431 t
ORIGIN      
        1 gtggatgaaa tatgaataac gaaactatca ttttaggaaa aaaatatatt tgccaaccta
       61 ttggcttgaa acaaaaagtg gttggagaag taatcagtaa attagaaaat tgtgttgtga
      121 tctgcgtaga cgaatatcat gacgtcgatc gcaatgaaat attagaaaaa tgcggaaaag
      181 tagtggcaaa atatactcac gtttatgagt tggaaacaac agaatatcta tataactata
      241 gcaacaaata tgaacatact ttagtttcat aaagatttaa ataaagtgcc ttcaaaagcg
      301 agaagaccaa gcttttgaag gcactttatt tagttggcag tgattgttca tgatttttga
      361 tttattcaaa aggttcgcct agagaatctg tttctgtaac gaggcctggt ggagaaactc
      421 gataaaaacc tatactgcca gaactgtcct attttcggat agacaaacta gtagttttaa
      481 aaagatcatc cgatccaatc atttcaaaaa atgcaaaact gatatcagga tcatttagct
      541 caggcttgtt atcgtaaagc atttgtaaag gttgcattta gttaggagta aacgcgaaac
      601 tatcatcatt atttgctgaa gaagatgaag aagctaattc agagtttgtt tgaaataaag
      661 gagacgtgct gacagtttct ttaccctgtt ttttgcagag gtagaaactt ttatatcgga
      721 agtttctacc ggagaatttt tctcatttgt agaaattgtt gaatcgatac tattcgaaat
      781 ttgcgtatct gaaggtatgt tctttgatgc agaagtccca caagcagtaa gtaacaataa
      841 atttgataaa ctgataaaca gaattctttt cattatgtac acactcctta tagtatcgtt
      901 tagcatacac ctatgctcag tattaggcaa aatcaatgca aaactatcaa gtcttttata
      961 gtatctatta gtcttagtaa tacatatatg gagagggtga tatcccagtt caaaccagat
     1021 acattggtta ccatctgtat taacgattat gcttctaaac gcttttttta tgaaaaaatt
     1081 tataagtgaa aacgtaggta tgatattatc tagaggtaat ttttttgggt atttaaatag
     1141 ttgctaggag gatagcggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
     1201 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
     1261 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
     1321 agtga
//
LOCUS       DANKHK010000197         1283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000197 DANKHK010000000
VERSION     DANKHK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            16..927
                     /locus_tag="PW109_002585"
     CDS             16..927
                     /locus_tag="PW109_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2512349.1"
                     /translation="MELSQENLRIVFEYRNVVRRPLTITVANSKGGVGKTTIIRYLSY
                     VLANMGFKVLVVDADPQSNTTKTMLLTKNYHSKDDDIFIIEKTMLAGIVERDLKQLVL
                     PIIENLDCIPSHIDFKNFPKYLTKLYGDAIEGIDADYIEVEKNRISVLKDLLEPVKPD
                     YDFVLIDTPPTMSDFTRNAAYASDYIIMAFQTQSDSLDGVEDYISEEVTPLVEKFDCK
                     TEIVGILPNQLSKGSIDRQVVNDAIEKFGDQNLFDNILPHVKRVQTTPRTGLNTDTYW
                     DKLAYEEVFVPLAKDFIHRIKLFEGEN"
     gene            929..>1283
                     /locus_tag="PW109_002586"
     CDS             929..>1283
                     /locus_tag="PW109_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512350.1"
                     /translation="MTDNAKPGFFSKQKPDNSYKEIPKPKSAFTSDNTDLLENIHPEE
                     DVLKINNKSLGVPAIVWCEFTALLDTTDNDYTYELLEELIHERTARFDHNQEITYKRI
                     LERKKDQERERIYKKE"
BASE COUNT          469 a          207 c          229 g          378 t
ORIGIN      
        1 cgggaggttt gtttaatgga attatcgcaa gaaaacttga gaattgtttt tgaatataga
       61 aatgtcgtta gaagaccact aactattact gtagcaaatt ccaagggtgg agtaggtaaa
      121 actactatta taagatactt gagctacgta cttgctaata tgggatttaa agtgttggta
      181 gtggatgccg atccgcaatc aaatactaca aagacaatgt tgttgactaa aaattatcac
      241 tctaaagacg atgacatttt cattattgaa aaaactatgc tagctggaat cgttgaacga
      301 gatttaaaac aattagttct tccaattatt gaaaacttgg attgcattcc atcacatata
      361 gattttaaaa actttccaaa atatttaact aagctttatg gggacgctat agaaggaata
      421 gacgctgatt atatcgaggt cgaaaaaaac agaatttcag tcttgaaaga tttacttgag
      481 ccagtcaagc cggattacga ttttgttctc atcgatactc cacctactat gtctgatttc
      541 actaggaatg ctgcttacgc ttctgactat attattatgg cttttcaaac acaatccgac
      601 agcttagatg gcgttgaaga ttatatatca gaagaagtca ctcctcttgt tgaaaaattt
      661 gattgtaaaa ctgaaatagt tggaatactt ccaaatcagt tgagtaaagg tagtatagat
      721 cgccaagttg tcaacgatgc tatcgaaaag tttggtgatc aaaacctttt tgataatatt
      781 ttaccacatg ttaagcgagt gcaaacaaca ccgaggacag gtctaaatac tgatacttat
      841 tgggacaaac ttgcttacga agaagttttt gtacctttag caaaagattt cattcataga
      901 ataaaattat tcgaaggaga aaactaacat gacagataat gcaaaaccag gtttcttttc
      961 taagcaaaaa cctgataatt cttataaaga gatccctaaa ccgaaatcag catttacttc
     1021 tgacaacaca gatttactag aaaatattca tcctgaagaa gatgtattaa agataaacaa
     1081 caagtctctt ggtgtaccag ccatagtctg gtgcgaattt acggctttgc ttgatacaac
     1141 tgataatgat tatacctatg aactactcga agaattaatt cacgaaagaa ctgcacgttt
     1201 cgatcacaat caagaaataa cctacaaacg aatattagag cggaagaaag atcaggaaag
     1261 agaacgtata tataagaaag aaa
//
LOCUS       DANKHK010000198         1227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000198 DANKHK010000000
VERSION     DANKHK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            64..330
                     /locus_tag="PW109_002587"
     CDS             64..330
                     /locus_tag="PW109_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2512351.1"
                     /translation="MTSTYASFDKDNLYYDFPPVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            412..708
                     /locus_tag="PW109_002588"
     CDS             412..708
                     /locus_tag="PW109_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512352.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFIMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
BASE COUNT          423 a          198 c          214 g          392 t
ORIGIN      
        1 acaatcaacc aaactctaac agaatttgaa gtatatcaag aatattggga accagtaaaa
       61 aaagtgactt caacttatgc atcctttgat aaagacaatc tctactatga tttcccccca
      121 gtatcaggat ttagttcaat gaacaataaa aaatatttac taatgttaac gagattaggg
      181 tactcaacaa gtaacgtccg tgctaaacgg aatggtggta cagttattac ggatattttg
      241 ctaagcaacc aataccaagt aattcctaat caagaaatcc agcatcaagc gactagcggt
      301 gtaaagatta tagaaagcga agtgagttaa acctcagata ttgcttcttt ttcatttgac
      361 gttctagaag taacaaaaga tgagttactt tatgtttatt ttcccgtcaa cttggccaat
      421 aatttgcact atgtgaccaa tttggaaagt cgacaatttg atccctgctg gcatcaaatt
      481 gatgctcaaa cgaaagaatt gttgctaggt tgggaagaaa atcaagtagt tcgtgatttc
      541 tcattaaatt ttattatgat tgattactat aatttggcga catcaatgca acggttcccg
      601 aattatggta tggacataga ttcattcaaa cagaaaagct tttctggaga aattcaaaat
      661 gaggaaagca ataatcttgt tatacctatt ctctatgaac caggttaggt ttttactgtg
      721 gacggggaag agtatcctta caaagtagct tttgacgatt tgattcaatt gtctaactta
      781 ccaaaagata gtaatatgaa aattgctgga gaatttttcg caccaggatt cttggtaggc
      841 ttggtaatct ctagtattgc aactgggtgc tttttatttt tactttttct ggattatatc
      901 aagaaaacag aggatattcc ttaggaaaat cacaatctaa atcttcccta gaaactatag
      961 aatgacaagg gggatataag caaattccta aatgattaaa tagggtgcgt tactagttag
     1021 ctccatagta agattttttc aaaattttct tgaggttgga tcaaaaaggg ctcttttcaa
     1081 ttagattaaa gacactcata caaaatagag aaacgaactt ataatcgcgt tcgtttccct
     1141 atttaattta ttatgaccat aaaccaattt gaatttagaa agcaccttct ttctttaata
     1201 aaaagtacta ctcgtaatct gtcagga
//
LOCUS       DANKHK010000199         1222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000199 DANKHK010000000
VERSION     DANKHK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            25..978
                     /locus_tag="PW109_002589"
     CDS             25..978
                     /locus_tag="PW109_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2512353.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            950..>1222
                     /locus_tag="PW109_002590"
     CDS             950..>1222
                     /locus_tag="PW109_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2512354.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          440 a          189 c          235 g          358 t
ORIGIN      
        1 tagtatagga aattggaggg aactatggaa aacttacgga ctgcatttaa aacatcagat
       61 aaactaggaa aagttgtaat gcttacaggt agaaaaggcg gcattggtaa gactacagac
      121 aatgatttat tagcgattgt ttctagtcaa ctttttgaaa aagacgtgtt gctaattgat
      181 tatgaccaac aaagaaatac tacttccaac attggatcaa cgtatcaaat tactagcttt
      241 gatcgatcca tgtctgcagc aatcaaaaaa ggggactggg tttcaggtat tactcaagta
      301 agtccacatt tatatattat ggctggctct cctggatctg aagaacttaa tgaatacttg
      361 tccgaaaaat atcctgatcg tcgtaagaga tcattggcct tcattaaacc tttagaagaa
      421 ttgagaaaaa atttcgatta tatctttatc gactgcccac catcaacaga taatgtggtt
      481 cgtgcattct taactgctgc agattatatt attgcaatgc aagaattaaa acggtacgca
      541 atggaaggaa cagaagattt tattaataaa gttttagttc cgattgtcac gaattttgaa
      601 gagtctcatc ttcaaatcat tggaattttg cctgttcttt tctcagtgcg acgttcctct
      661 caacacgcaa actatcaaaa aacaattgat aagtatggtg aatcaaacat ttttaaaagc
      721 attgttaaag gctcggatcg gttagaaatg tatggtgaga atggtatcca attaaatgat
      781 tatgtagatc gaagatggtg ggctattttt gcagacatct ttacagaatt agaagaaaga
      841 attgaatact atgaacaaca tggtgatatt gaaggatttg attatacacc taaatatgct
      901 gactcaatgg ccaatcatat cctacctctt ggaaaggaga ttaaactaaa tggcattatt
      961 accaacgaac aaagataaga agaaaattgg tgctgaagat atttcaatta aagatgatat
     1021 taatgtcgag ttaggggaac gaaagtacac tgcgaaagaa agaaaaccag ttcaagtaga
     1081 tcctcccgta ctaaaaacca ttcgggatct tagttatgca aaagatattc ctatgtatga
     1141 aatagtcaaa cttgcaatgg acgcctatgt tgatacttta aacgacgagg aacggattat
     1201 atataataga cgatcaaaag at
//
LOCUS       DANKHK010000200         1205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000200 DANKHK010000000
VERSION     DANKHK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(34..1128)
                     /locus_tag="PW109_002591"
     CDS             complement(34..1128)
                     /locus_tag="PW109_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2512355.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          343 a          262 c          192 g          408 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acacc
//
LOCUS       DANKHK010000201         1192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000201 DANKHK010000000
VERSION     DANKHK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            20..829
                     /locus_tag="PW109_002592"
     CDS             20..829
                     /locus_tag="PW109_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2512356.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQKDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESGDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          403 a          217 c          232 g          340 t
ORIGIN      
        1 aagaaagtag gaagataaaa tgaaactagc agcgattgat ttagacggta ccttacttga
       61 tcgaaatgga aagataccag aagaaaatat ccaagcactt cggaaatttg atgaaaatgg
      121 cggaatcgta accgtcgcta ccggaaggaa cagcatttct gctaaagata ttttttccga
      181 attaggaatc ggtggctatt tgatttcttc caatggttca ctgattgctg aaatgaagga
      241 tggaaaaatg gcgaaagttc tcagacgttc aaaaatagat agcgatacac tgagaaaagc
      301 cttcttctat gctcaagaag cgaaaatatc catcattgct agccgagaaa cccaagatga
      361 tcagctgaca ttcgatgaat caaatctagt caaagatgac ccttattatg aacatttcaa
      421 tttgcagaac cattcttttg atcaaatttt aaatatgttg gaagatcctt ctcttagcta
      481 tcttaaatta gcattaacag ataagagtga agaaaaacta cagaagctac aaaaagatct
      541 gcaagcagat ggtatccaat cagtattctc tgatccgcat ttcttagaaa tcacgcctac
      601 aggtatcact aaagctcatt cacttgtttt tctggcggat taccttggaa tcgaatcggg
      661 tgacatcatg gcttttggcg atcaagaaaa cgacattgct atgttgaatt acagcgggtt
      721 gagtattgcg atggggaatg cacagcggca agtaaaagaa attgcaaacc aagtaacgct
      781 gacgaatgaa gaagctggtg ttgcttatat tttaaaaaac ttctattgaa aataaaaaaa
      841 tatttagcac acaataaaaa aagcacgcag aactgtttta gtcctacgtg ctttttctat
      901 caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga cctctttctt
      961 aaatggtgtt caatcagctg accctcggta ttagcttgac aaacggcaaa atatgaggaa
     1021 ctatttttcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
     1081 ttaaacggta ttcaaaagct tgcatctgca gtaataagtc cagccaagta aaaaacataa
     1141 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tc
//
LOCUS       DANKHK010000202         1182 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000202 DANKHK010000000
VERSION     DANKHK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            195..829
                     /locus_tag="PW109_002593"
                     /pseudo
     CDS             195..829
                     /locus_tag="PW109_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742338.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            932..1182
                     /locus_tag="PW109_002594"
                     /pseudo
     CDS             932..1182
                     /locus_tag="PW109_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741239.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          394 a          171 c          258 g          359 t
ORIGIN      
        1 ttttaaaacg agtgaaagtc attttcaaaa aagaaagcaa aaagtgaaaa cgctaccctt
       61 tttaggcttc gtctatcgaa gtgccgcaac tcggatggta aggttgaact gtatcaatca
      121 gctgatggtc tttgctagtt acttttgttg ttgtgactgg catacgaata attgttgaac
      181 aggaggaaat ttttatgtta gttattgcct atattggttt tggtaacagt gtttgtcaat
      241 atcacttacc ttatgtagag gctcgtaaag aaaagatgaa ggttaaatat atttatcgca
      301 gagaagaaga ccgaaaagga gatatggagc gtatccaacg attcaattta cttcttcgat
      361 tgaagaagta atgcaggatt cagaggtaaa tctaattgtg gtcaacacac cagaccaatt
      421 ccatgttgcc tataccaaaa tggctctaga gaatggaaaa aacgttctta cagaaaaacc
      481 atttgcgatg actggtgaag aagcgaggga agtttttgca cttgcaaaag aaaaaggctt
      541 attggcgatg gctaatcaaa atcgacgttt tgatgcagat atgcgaacgg ttcgaaaagt
      601 gatcgaatca ggagttttag gtaaattgat tgaagtggaa tctcactatg attactatcg
      661 tccggaaatt gttaatcata aaggaatggg tgctctttac gggttagcag ttcatccaat
      721 tgatcagatt gttggggagt ttggagttcc gaagagactt gtatatgatg tgcgtagtat
      781 tgataaccca ggaatttctg atgattatta tgacattgat tttttctatt gtaaagggcc
      841 acgataaaat gtaggaaaaa ggccatcgaa aatgtaggct gaattcaaat ctgataatct
      901 ttttcatgaa aacattcaag gagagtgaat catgagaaaa gatgtcttag aaggagtcct
      961 acgccacatt atgaacgata ttcaaccaaa ttacgcagct ttggctaaac aatacaattg
     1021 tgattacaga actgttaaac gctattacga agctggtaga aaaggaaata tagaccagct
     1081 aaaaaaagaa agtcttcaag ttctccttta ctttctggat ttgaagagat tattagagat
     1141 aaattggagt tgagctgttc tgcttcttct atttattact tt
//
LOCUS       DANKHK010000203         1150 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000203 DANKHK010000000
VERSION     DANKHK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            73..>1150
                     /locus_tag="PW109_002595"
     CDS             73..>1150
                     /locus_tag="PW109_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDL2512357.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT          365 a          214 c          229 g          342 t
ORIGIN      
        1 agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat
       61 ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga
      121 aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct cgttcgaaag
      181 attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac ttactccgat
      241 ttaggtcgcc caagtatcga tccagtgatt ctgatcaaat tggtgtttat tcagtattta
      301 ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc
      361 tggtttttag gctactcctt cgaggagaaa atcccccatt tctcaacttt tggaaagaat
      421 tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta tattttagaa
      481 caagcagtta aagcaggttt tgtaacagag gataatttgt atctggattc tacccacatc
      541 aaagctaacg ccaataaaca taagtttacg aaagaaatga cccatgatga agcgaaagcc
      601 tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct
      661 tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat
      721 ccggaaagcg gctactacgt caaaggcgaa cgagaaaaac aatttgctta ttctgcccat
      781 acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg aaatattcat
      841 gacagtcaag tcgcctttca gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat
      901 tgtgttgtcg ctgatgctgg gtataagaca cctaagtttg ttcacttttt gactcatttg
      961 aaccttcgac cttgtttgcc ttattcaagg cccaaaggga aaaaggggct attatcaaaa
     1021 aatgagtttc tctatgacga gtattttgat tgttatattt gtccgcaaga tcaaatgctc
     1081 gctttttcaa cggttactcg agaagggtac cgagaataca aatccaatcc aaaagaatgt
     1141 gtgaactgtc
//
LOCUS       DANKHK010000204         1108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000204 DANKHK010000000
VERSION     DANKHK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..148
                     /locus_tag="PW109_002596"
     CDS             <1..148
                     /locus_tag="PW109_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2512358.1"
                     /translation="SKMYMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKFS
                     GIKQ"
     gene            complement(262..891)
                     /locus_tag="PW109_002597"
     CDS             complement(262..891)
                     /locus_tag="PW109_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2512359.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1025..1097)
                     /locus_tag="PW109_002598"
     tRNA            complement(1025..1097)
                     /locus_tag="PW109_002598"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1062..1064),aa:Thr,seq:ggt)
BASE COUNT          363 a          188 c          190 g          367 t
ORIGIN      
        1 ttcaaaaatg tacatggatg aacatatgac cctttcttta gatccacgtc taggtgtatt
       61 tactcttgaa agcccaagtg gttctttaga cagtcatgtt caattagtag atctacgacc
      121 agaaaaattc tctggaataa aacaataact ttctctctat tgtccataaa actagtctca
      181 atcaagctgt attgaaaaaa caagacaaca ttcttaccat ggtaaataaa aagacgacac
      241 attttgtcgt ctttttttaa attaactatt ttcgataaaa gatcgcatag cttgtgccat
      301 tatttctggc ttttctctat tcaattcatg ttttccgttt ttaataaaga gtagccggcc
      361 attcgttgaa agtttatata aacgttttgc tgctggttta ttcgcatgat ctttggtacc
      421 gcataaaatc aatttaggaa gtcgtgatgt acgaagctgt tttttgcagt ctaatgtttc
      481 aagtgaacga agtagggtaa gggtttgttt tttagataga cctaattttt caaatatctt
      541 agaaggtacg cagtaaaaat aaatattctg gaaagtaagc aataagcgat taggagactc
      601 aaattgtgga gcgactaaaa caaccttttt taaatatgga gaaggagttg caagatagtt
      661 taaagcaata acagcgccta aagataatcc acataaaata aaaggttcct ttatctgatt
      721 caattgtact tcgatgtatt tggttaagtc gtctaaagta gcagtgtctg tgctgtttaa
      781 atgattgaac aatgaaagac ttttgcaaga ataactattg ggtagataag agattacatc
      841 tttccatgat tgttcgtctt ggcctagacc atgcaaaaaa acaattttca tattgctctc
      901 ctttttatag ttatcatata gactttcttt gtactattaa aatataaaca ttcatttctt
      961 acgtcataaa ataaaaaaac agcgcagaca cattgttaaa ccaacatgtt gcactgttta
     1021 aggaagccac ttgccgggct tgaaccggcg acctcttcct taccatggaa gcgctctacc
     1081 agctgagcta aagcggcatt atcattaa
//
LOCUS       DANKHK010000205         1107 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000205 DANKHK010000000
VERSION     DANKHK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            5..919
                     /locus_tag="PW109_002599"
                     /pseudo
     CDS             5..919
                     /locus_tag="PW109_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(973..>1107)
                     /locus_tag="PW109_002600"
     CDS             complement(973..>1107)
                     /locus_tag="PW109_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2512360.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          388 a          163 c          192 g          364 t
ORIGIN      
        1 aaaaatgtta aaagatttaa aacagtatta tcctctgtct gcttatgaaa aaaagaaatt
       61 attccacttt ttagcaatca acctgttggc tgaacaagct gattctttag atattgctag
      121 caatcagtcg ctattttttt cggatatagg attttaccac ttaaaaggag tattaggaga
      181 aaaatccgtt atttttatca ataagacact ctcttggctt tatcttcatg ataaattgga
      241 tcaaaaatac ttgagaaaaa atgaagatga aacaatcgaa tggttgaatg aatgattcat
      301 tgaaagtttt gaaaatcagt ttaatctgtt gcgagaagag acgagccaat tgatccgaag
      361 agagttaacc aaaatacatt ttaaactttt gcattacact attaataaag ctgatgaatt
      421 tccacttgat gtttcttttt ttagagaaaa ctatccagca ttttattgct atttgataga
      481 gtatataaat gatctaacgt caaaacgcaa agagttgctt caagataaat tttttctttt
      541 tttcagttat ttattattat tgatcaatca catacccatt caactgattt ctgaaccagt
      601 aaagatcatc atcgattttt cttatggtgc tgcctataat caatttatca agaaaaattt
      661 aagcttgtac attaatctga acacggaagt gatagaggca ggaaagcctg agttcccgga
      721 tgttataatc acgaatagga ataatctata tgaagaaagt gcatctcaag tagtggtttg
      781 gctcgatccg ccaagagcag tagactgggg gaatcttaca aagattttgt tgactatcca
      841 agaagagaaa tacaaagaca gcaaaattga caagcaaata gacgattttc ctgaagaaat
      901 catcgacagg atcgaatagt gaacttcttt tagaaagtcc gactgctttt tctaaaaata
      961 ttatttgtca aattaagcga gacaaaactt tccatttacg taactcaaaa aaacttctaa
     1021 tggtgtttgg taatttagtg attttctagg gatatggttt cttttggacg caacggatga
     1081 gatgaatcct tgattgactt ggttgaa
//
LOCUS       DANKHK010000206         1081 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000206 DANKHK010000000
VERSION     DANKHK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(57..866)
                     /locus_tag="PW109_002601"
     CDS             complement(57..866)
                     /locus_tag="PW109_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2512361.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          332 a          204 c          190 g          355 t
ORIGIN      
        1 aaaaaggttg tgacaaaaat tgtcacaacc tcttttttgt ggaatctaat tctctattat
       61 agtttaagta gtttttccag ataaacagct aaaccgtctt cctgattgct caaaggagtg
      121 acatcatttg caatgccttt gagttggtca gtaccgttag ccatagctac tccccaacct
      181 gcataatcca acaattccaa atcattatgc tcgtcaccaa aggcaatgac gtctttttga
      241 tcgatagaca ggtagttagc aatctcttgc acgccttttg ctttttggat tccttttgaa
      301 acaatctcta agatggcagt aggtccaccc catgtccgaa catcgacata actgccaaat
      361 tgacgtgtta attcagcaga tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa
      421 gatgtaggat ttgtcgtcaa atttttagga gaaagcaaat tcttctctcc tggacgagaa
      481 gaagcaaaaa ttttttcatc aaagaaatca aaactgtcaa taaaaaaagt atcacgattt
      541 tcagcagcga taaaatctaa atttagctgc tttttttgcg ataagatttc aaaagcgatt
      601 tcacgattga tcaacgtttc tctttcacca tcccagtgct gattagggat gtgtactaat
      661 gcgccgttaa aattaaccat cggtgttgtt aattctaatt gttggtaaaa ctgatggctc
      721 attcggaatg ggcgtcctgt agcaatactg acatgatgac cggcattgat tgcttttttc
      781 agagtctctt ttgttttaga tgtaatcatc gaatcttgat tgagggtagt tccgtccaga
      841 tcgatggcaa ttaatttttt attcaataag ggtccttcct tcctcaacta gcatttgttt
      901 tagtctagca tattttaggt aattaacaag agcaatcagc tattcctaac aaaaactaca
      961 gttatggcta tataaatagc taaacataag ctaaacacga acgttaagta ttattttcgc
     1021 tttttttgtt aaaaaaacgt tgaaaagcga atgaaacgtc tatataattt gaaattgttc
     1081 g
//
LOCUS       DANKHK010000207         1042 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000207 DANKHK010000000
VERSION     DANKHK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="PW109_002602"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="PW109_002602"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="PW109_002603"
     tRNA            203..276
                     /locus_tag="PW109_002603"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="PW109_002604"
     tRNA            316..405
                     /locus_tag="PW109_002604"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="PW109_002605"
     tRNA            420..491
                     /locus_tag="PW109_002605"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="PW109_002606"
     tRNA            507..579
                     /locus_tag="PW109_002606"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="PW109_002607"
     tRNA            589..661
                     /locus_tag="PW109_002607"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="PW109_002608"
     tRNA            697..772
                     /locus_tag="PW109_002608"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="PW109_002609"
     tRNA            774..854
                     /locus_tag="PW109_002609"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="PW109_002610"
     tRNA            861..934
                     /locus_tag="PW109_002610"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="PW109_002611"
     tRNA            937..1009
                     /locus_tag="PW109_002611"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          247 a          232 c          281 g          282 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta ag
//
LOCUS       DANKHK010000208         1030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000208 DANKHK010000000
VERSION     DANKHK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            92..>1030
                     /gene="bsh"
                     /locus_tag="PW109_002612"
     CDS             92..>1030
                     /gene="bsh"
                     /locus_tag="PW109_002612"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2512362.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYP"
BASE COUNT          353 a          134 c          190 g          353 t
ORIGIN      
        1 ttctttgtgt attctgtgct tttcttttgg aatgaggaaa actttggagt aatatgaacg
       61 tggacagaaa aatatttagg aggaataaac tatgtgtacg tctattactt atgtaacaag
      121 tgatcattat tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactat
      181 tactccaaga aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc
      241 aatgattggt attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga
      301 aaaaggattg agtatggctg gactaaattt ttctgggtat gctgattata aagaaataca
      361 agaagggaaa gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc
      421 aacagtagga gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag
      481 tgacgaactt cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt
      541 cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa
      601 taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga
      661 aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg
      721 agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac
      781 gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat
      841 cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata
      901 tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga
      961 agacagtcaa attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat
     1021 tagttatcca
//
LOCUS       DANKHK010000209          994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000209 DANKHK010000000
VERSION     DANKHK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..>994)
                     /locus_tag="PW109_002613"
     rRNA            complement(<1..>994)
                     /locus_tag="PW109_002613"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DANKHK010000210          979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000210 DANKHK010000000
VERSION     DANKHK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..951
                     /locus_tag="PW109_002614"
     CDS             <1..951
                     /locus_tag="PW109_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2512363.1"
                     /translation="KKMKDSTKIAYMLLKARLEIAISKRQIDEEGNVYFTYTTNELCS
                     VLNCQKQKAIAIKKELESFGLLLQKQMGFNKQLGKNNPNRLYLAELKVSENDIYLLEK
                     FDRENRENVDKSEGMKIIPTLDEKSDAESLGAQEGMKIIPCQNVDKSEGMKIIPELNN
                     NILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIATFVPERVLNLIKTFSSSYSEAQ
                     KTVQTIHNAKKKAEIESGISIVFEELDSYYVNAEQELYTTLLKAYQKLKTEKVENIQN
                     LIFVYVKNWFIEKPIAAKVSSEKRLNSESSPSTITKDWLE"
BASE COUNT          405 a          152 c          169 g          253 t
ORIGIN      
        1 aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc
       61 gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat
      121 gaactatgta gcgtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg
      181 gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat
      241 aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc
      301 gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata
      361 cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc
      421 ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat
      481 aatatattag acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa
      541 ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct
      601 gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc
      661 gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat ttcgatagtt
      721 tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta
      781 aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc
      841 tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt
      901 ttgaattctg aaagctcccc aagcactatt acgaaagact ggttagagtg aaactttact
      961 gacaaagtga ttgttgctt
//
LOCUS       DANKHK010000211          974 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000211 DANKHK010000000
VERSION     DANKHK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..220
                     /locus_tag="PW109_002615"
     CDS             <1..220
                     /locus_tag="PW109_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2512364.1"
                     /translation="EHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRG
                     IYKKNRRNGMLFGFSVSTEIKVLMGITA"
     gene            282..899
                     /locus_tag="PW109_002616"
     CDS             282..899
                     /locus_tag="PW109_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512365.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVMKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          361 a          140 c          185 g          288 t
ORIGIN      
        1 agagcaccga actgtgaagt atcttaacaa tttaatagaa caagaccatc gacctattaa
       61 acgacgaaat aaattttatc aaagtctccg tacagcctct tccacgatta agggcatgga
      121 aaccattcga ggaatatata aaaagaaccg aagaaatgga atgctcttcg gcttttcggt
      181 gtctactgaa atcaaggtat taatgggaat aacagcctaa gatatttgga gttcagagag
      241 ggcgcgtttg attttcaaac ttcgcaacag aaccgagaac cttgggtttt caagtgcttt
      301 ttggttcatt aatcttaacc atagtgtttt taaagtggaa aacaagcaac aaaaaaagta
      361 tgtggagcct tgtaggaata gttcttatta tttttactgt gactgcagca acagaagcaa
      421 attattcgca taaaattcag tcgtataaag gtcgtttagt tatgaccaaa aaagatgtta
      481 cacgttatac atcctcaaga actggaggcc tatatgacta tgcaccatta gatgcattga
      541 aatataaaga tcatttaaag aaacatgaag taaaagttgg tgatcggtgg gagaatgcat
      601 ctcataaagc atataattcc aaaatagtct atacagtaga agatgccgat gagcaaaata
      661 tcacattacc tgtattcaat tattggggaa cagttatgaa agtaaatggg aaaaagagca
      721 ctatagaaaa tgacaatggt ttagtgtcta ttaaaggtac ggggaaaaga actatagttg
      781 aggtttcatc tacgtatcca actacgatga ttctatcttt attaattagt tcaataactt
      841 ttatattctt atttacacgt tatttaataa gatgctgcgg tgaaatgaaa atagattaga
      901 tagatattat aaatatgttg attctatttt catagcaatg gagaaaatac attagcaata
      961 tataaaagaa ggta
//
LOCUS       DANKHK010000212          969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000212 DANKHK010000000
VERSION     DANKHK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            12..422
                     /gene="spx"
                     /locus_tag="PW109_002617"
     CDS             12..422
                     /gene="spx"
                     /locus_tag="PW109_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2512366.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="PW109_002618"
     CDS             436..792
                     /locus_tag="PW109_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2512367.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          329 a          153 c          193 g          294 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattg
//
LOCUS       DANKHK010000213          935 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000213 DANKHK010000000
VERSION     DANKHK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            153..482
                     /locus_tag="PW109_002619"
     CDS             153..482
                     /locus_tag="PW109_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2512368.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            472..759
                     /locus_tag="PW109_002620"
     CDS             472..759
                     /locus_tag="PW109_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2512369.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHEKFYEQLKKLI"
BASE COUNT          343 a          118 c          170 g          304 t
ORIGIN      
        1 ttaaaacttt gcaacagaac cagatgaaca cgtggttgaa atttattcag caaggacgca
       61 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
      121 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      181 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      241 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      301 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      361 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      421 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      481 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      541 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      601 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      661 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      721 catgaaaaat tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      781 aaggacggct tcttttttat cgaaaatctg attcgtctat atgactaaag tcgcgagtat
      841 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      901 atttcagctt ttccttattt atgtactacc ttttc
//
LOCUS       DANKHK010000214          907 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000214 DANKHK010000000
VERSION     DANKHK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            227..859
                     /locus_tag="PW109_002621"
     CDS             227..859
                     /locus_tag="PW109_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2512370.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          328 a          124 c          137 g          318 t
ORIGIN      
        1 gatttttatt ttaaaaacat tctaaattaa atcagtaaaa atttttattc taagattttt
       61 attagaatta cgcctaattt gtgtctctca gggcaattct aagaacgttt caagagatag
      121 cgacactatc gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg
      181 tttagctttt gttaatatga ctttatgaaa atggaggtaa ttaaatatga tttttcctaa
      241 agttaatgaa gcaagcttga ataaaggatc cttttttcat atctatgaaa acgaagattt
      301 tgaactgtat aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc
      361 aattatttac aaatctattt tcaagtatgc attttttgct tccaatgatt ttcaaagaat
      421 ggagattacc gactgtatat tcgaaaactg tgatttttca ggctgtatat tgacaaaaag
      481 catgttgcat cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat tgatggaaag
      541 ttatattggg aacacgttat tcgagaattg taaaatggac tatgtaaatt tatctggttc
      601 aaacataaaa gaaagtacct ttgagcactc tgtcttatca agcgcagatt ttgtagactg
      661 ttccctcact aaaataatgt tttctactaa tgatttagaa aatgctagct tttttgatac
      721 tgatttaaaa aatatcgatt taagtagcaa ctcctttgaa atgattgaag tacgctcgga
      781 acatctaaaa aattgtacgc ttaatcagtt gcaagcactt ggttttgcta gaaaattttt
      841 acaaataaaa gtagtataat caaaatgaat atataagata aatttttagc aatacaaata
      901 tccataa
//
LOCUS       DANKHK010000215          890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000215 DANKHK010000000
VERSION     DANKHK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..888
                     /locus_tag="PW109_002622"
     CDS             <1..888
                     /locus_tag="PW109_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2512371.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DANKHK010000216          869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000216 DANKHK010000000
VERSION     DANKHK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            433..>869
                     /locus_tag="PW109_002623"
     CDS             433..>869
                     /locus_tag="PW109_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2512372.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDE"
BASE COUNT          300 a          156 c          131 g          282 t
ORIGIN      
        1 ttttaacttc taactgttct ttattaatta aaaatgttct gtaataaaat aaaactttat
       61 aacaactata acaaaaccga atgcaaaaca gaagttctat aagttataat catagttgtt
      121 aggtgattca tctagtaaaa acaccagatt tcaaatttca cacaatttaa actagatcaa
      181 tcatcaacct tcttcaaatt gttagaaccc tttcccgagg agctgcagca taagcaaaaa
      241 aacaaacagg aagccaaatg gctaagacat tttttctatt agtctagttg tttctcgtcg
      301 caagtttaag actttagaaa gttatttcta atgatgtttg ggttgttttg atcaatgctt
      361 atgcacagct ccttacctat tatttatctc aacagatcta acaaattaat ggactattta
      421 aggagttttt ttatgcttac gatctattct tctcgcacac atagttacca tctgatggca
      481 aactggctga aagaccatca aatgccttac agagaacgaa ttttcacacc taaacagcct
      541 ttaacaagga aagagatatt ttatttattg tcattaacag aaaacggatt tgacgatcta
      601 ttagctaaac gctcgattga ttataaaaat ttgaaagacc aaatcgacca atgtacgacc
      661 tcagagcttg tcgatctgat cgtctcgcat tttactttgt tacaacgtcc catcattact
      721 gacggaaaga aattattagt cggattcaat gaagatagca tgcgtctttt tatccctgta
      781 caatggcgca gaagtcaact gaaatctgtc tataacgatt ttacatgggt aagcaatcta
      841 gaaaattcta cggatttaga tgatgaatt
//
LOCUS       DANKHK010000217          834 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000217 DANKHK010000000
VERSION     DANKHK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..252)
                     /locus_tag="PW109_002624"
     CDS             complement(<1..252)
                     /locus_tag="PW109_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512373.1"
                     /translation="MIFENGSGGTGRMAEENKVVPFETLVINKEEFEQLFLNFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELF"
     gene            complement(293..787)
                     /locus_tag="PW109_002625"
     CDS             complement(293..787)
                     /locus_tag="PW109_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512374.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKAETINYYYELPNEMQDYQIVLRRANGYLVGL
                     PFTL"
BASE COUNT          258 a          160 c           97 g          319 t
ORIGIN      
        1 gaataattca ttgaattttt ctactgaaat aatgatttta ccattcttct ctcgactgtt
       61 ttctattatt tctgcttcta attcaaataa atctagtgac gagctaaaat cataaatcct
      121 gtcatattga ataaaaaaat taagaaaaag ttgctcaaac tcttccttat ttatcactaa
      181 tgtttcaaac ggaacaactt tattctcctc agccattctc ccagttcccc cacttccatt
      241 ttcaaaaatc acaaaactga tattacctta aaagctactg acaaaataaa acttataaag
      301 taaatggcaa cccaactagg taaccatttg ctctacgtaa tacgatttgg taatcctgca
      361 tttcattcgg taattcataa tagtaattta ttgtttctgc tttgtcactt tcaatatact
      421 ctggattatc taatatcata gcatactcat aaagctcttc accatcctta ttttcaatcg
      481 accattgttg tggacgtatt ggtttttctg tatcattttt aagtgtatag gtcacttgta
      541 atacacgatc atgttgtgca aattcatttc ttttttcggt cacttcaata tttttaaatg
      601 ttagttcaac cccatctgcc tgataagttt cacctatttt taaatctatt gttacaattt
      661 ttattggctt ttcttctgga aaatgtctgg ctttaattgt tagataactt ccaaaaagaa
      721 tcaatcctag tagcactaaa agaattcctt taacgagcca ctgaaacggg aagtaaactt
      781 taaacatctt ttttctcctc cgttttgtat ttgtttttta gagaatacga taac
//
LOCUS       DANKHK010000218          824 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000218 DANKHK010000000
VERSION     DANKHK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            76..>824
                     /locus_tag="PW109_002626"
     CDS             76..>824
                     /locus_tag="PW109_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2512375.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          298 a          149 c          149 g          228 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      361 cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      421 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      481 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      541 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      601 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      661 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      721 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      781 aaccaacatg atattgatat atattttgcg gaccctggaa cacc
//
LOCUS       DANKHK010000219          820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000219 DANKHK010000000
VERSION     DANKHK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(44..757)
                     /locus_tag="PW109_002627"
     CDS             complement(44..757)
                     /locus_tag="PW109_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2512376.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          259 a          165 c          108 g          288 t
ORIGIN      
        1 ttagtataaa aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa
       61 cagcttcaaa aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac
      121 cagacgatag agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta
      181 atatttttgc gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta
      241 tttccagacc cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt
      301 ccaagcctgg tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat
      361 caataaaacg gcttcgtaca atattatata catagtcttt tacactgata cttaatttat
      421 cagctacttt tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt
      481 cttcaccaac cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg
      541 gtgttctgac gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa
      601 gtaagtctac gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat
      661 ctcctgtagg aagtttctca ccaattttat actctccatt tacaattttt ttctttatat
      721 ctaagtaaat ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca
      781 tatcttcatc tatactttaa cataaataaa ccgataaaat
//
LOCUS       DANKHK010000220          813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000220 DANKHK010000000
VERSION     DANKHK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            101..>813
                     /locus_tag="PW109_002628"
     CDS             101..>813
                     /locus_tag="PW109_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2512377.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGL"
BASE COUNT          254 a          114 c          168 g          277 t
ORIGIN      
        1 attcaggtct ttcactaatt ggcgtgacaa tttttgcagc tctgattgcc tttgtaatgg
       61 atcaagttaa atatggaaaa agagaagagg cgcatggtta atggaaaata gtacagaaaa
      121 aaaacgttta atcacaaaaa aagatcttaa taagatgtgt cttcgttcat ggacaatcat
      181 gtgttcgtgg aattatgaaa gacaaatgca tatgggattt atgtatggga tggcgccaat
      241 tttaaataaa gtttataaaa atgatgaaga gaaaaaaaga gaagcttata ctcgtcatat
      301 ggcatttttt aactgtacgc ctcaaatgac ttcatttatt atgggacttt gtgcttctat
      361 ggaagaacaa aatgctcaag cagaagaagg aaactttaat actgaatcta tctccatgat
      421 taaaaccagt ttaatgggtc cttttgctgg aattggtgat tcatttttcc aaggtacttt
      481 aagaattatt acatttggga ttggattgtc ttttgctaag caaggtagta ttttaggacc
      541 gattttagct gttggccttt ttgctatacc ttctatttta gctgtttttt acggcggtaa
      601 attaggatat gagtctggca ataaatacct tactaaattg tatcaagaag gtcttatgga
      661 tcgagtaatg caatttgctt ctatcattgg tttggctgta gtaggtggaa tggtggctag
      721 catggtgcca attgtcaccc ctttaaaatt cactacaggg ggaacagaac ttgttttaca
      781 agacttactt gattccatta ttcctggatt att
//
LOCUS       DANKHK010000221          769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000221 DANKHK010000000
VERSION     DANKHK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..769)
                     /locus_tag="PW109_002629"
     CDS             complement(<1..769)
                     /locus_tag="PW109_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512378.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILN"
BASE COUNT          309 a          120 c          127 g          213 t
ORIGIN      
        1 tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct tttcccagtg
       61 ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat cccgcactta
      121 aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt ttttggtccc
      181 aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata gcgacagaaa
      241 gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat ttttggtaat
      301 ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt aaagcgacag
      361 cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa aatgtataaa
      421 taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata gaaaaataag
      481 aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt aaaacaagat
      541 ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga taaaaaatat
      601 tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc catacattat
      661 ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta tgatagattc
      721 ccttagacat gaatagaggg atgacgtaag caaaacttag aaataacat
//
LOCUS       DANKHK010000222          731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000222 DANKHK010000000
VERSION     DANKHK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PW109_002630"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PW109_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2512379.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="PW109_002631"
     CDS             complement(481..675)
                     /locus_tag="PW109_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512380.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DANKHK010000223          713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000223 DANKHK010000000
VERSION     DANKHK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            242..>713
                     /locus_tag="PW109_002632"
     CDS             242..>713
                     /locus_tag="PW109_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2512381.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKL"
BASE COUNT          248 a           97 c          150 g          218 t
ORIGIN      
        1 taaaacaaaa tactttggaa gatgaaattt atttcaatga aacgagtgag gaatttcaaa
       61 aatttcagac acattttaag caatctttca gtgtcgttga acattattat agtgtcgaca
      121 tcccgattca tgaggttaaa tacgtttatg attatgtaaa aagagcttga tttatcaagt
      181 tctttttgtt ttggcatggt aattgcttgt aagaaaatga aagataaagg aggaagtatt
      241 tatgagtatt gttatgtcga gagttgataa ccgtttattg catgggatta ttgtcacgca
      301 atgggcacca atgtctaaag caaacagagt gatggtgatc gatgattctg tcgcaaataa
      361 cgacatccaa aaatcaagta tgcgtttagc tagacctgct ggaatggctg tatcaattat
      421 cactgaagaa acagcgattg cgaattttaa aaaggggaaa tacgatcagg aaaaagtatt
      481 tgtcatcgtg aaagagccgg aaacaatcct tcatttagta gataacggta ttagaattga
      541 ccgactaatt gttggcggta ctgttgttaa agataatgca ttacagctat cttctagagc
      601 ctatgcatca gatgaaaata tcagcacgta taaaaaattg ttggattctg gtgtagaaat
      661 tgttacacaa tttgttccgg cggataaggc acaggaaatt caatcaaaat tag
//
LOCUS       DANKHK010000224          706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000224 DANKHK010000000
VERSION     DANKHK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(204..353)
                     /locus_tag="PW109_002633"
     CDS             complement(204..353)
                     /locus_tag="PW109_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512382.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          238 a          114 c          106 g          248 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatac
//
LOCUS       DANKHK010000225          673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000225 DANKHK010000000
VERSION     DANKHK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(46..672)
                     /locus_tag="PW109_002634"
     CDS             complement(46..672)
                     /locus_tag="PW109_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512383.1"
                     /translation="MQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLL
                     DHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLA
                     ILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFS
                     FFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          286 a           96 c          130 g          161 t
ORIGIN      
        1 attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt aatagtcagg cttcaaaatg
       61 ctttttattt tgtttgtttt ttctttgcca cataaataca cctagactga cgatgaagag
      121 catatccaac agatttcctg cgacttggat cgtcgaaggg ttggtcagtg gcattaaagc
      181 aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg acaggaacta atcgaaagaa
      241 gagcggacat ttcaattggc taaaaatgag tgaaaaaata aaataagtaa aaatggataa
      301 aatcacgact aaaccattca tgataaagaa gaaaagcgta ttgtgattaa acgaataaaa
      361 aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat acgcttgaag cttcgtttaa
      421 cgtaccagga tgtcgatgaa aaaaataggc accgaaataa acagaaaaca gtaagccgca
      481 aagagaagac aaaatcaaaa aaaacggatt ttcttcggct tctgtgatat cagaatgatc
      541 caaaagcaac cagcctaatg cactagaagg cgcatgtgag gtcttaaggt atgaataaca
      601 tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct aaatgtaaaa aattagtggt
      661 tctacgttga att
//
LOCUS       DANKHK010000226          672 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000226 DANKHK010000000
VERSION     DANKHK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(352..576)
                     /locus_tag="PW109_002635"
     CDS             complement(352..576)
                     /locus_tag="PW109_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512384.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          249 a          100 c          120 g          203 t
ORIGIN      
        1 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
       61 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      121 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      181 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      241 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      301 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      361 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      421 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      481 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      541 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      601 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      661 aattataagg ga
//
LOCUS       DANKHK010000227          670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000227 DANKHK010000000
VERSION     DANKHK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..>670
                     /locus_tag="PW109_002636"
     rRNA            <1..>670
                     /locus_tag="PW109_002636"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          200 a          136 c          198 g          136 t
ORIGIN      
        1 agaaattccg tcggaatccg ggaggaccat ctcccaaggc taaatactcc ctagtgaccg
       61 atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cggaagggga gtgaaataga
      121 acctgaaacc gtgtgcctac aacaagtcaa agcccgttaa tgggtgatgg cgtgcctttt
      181 gtagaatgaa ccggcgagtt acgattgcat gcgaggttaa gttgaagaga cggagccgca
      241 gcgaaagcga gtctgaatag ggcgtttgag tatgtagtcg tagacccgaa accatgtgat
      301 ctacccatgt ccaggttgaa ggtgcggtaa aacgcactgg aggaccgaac ccacgtacgt
      361 tgaaaagtgc ggggatgagg tgtgggtagc ggagaaattc caaacgaact tggagatagc
      421 tggttctctc cgaaatagct ttagggctag cctcggaatt gagaatgatg gaggtagagc
      481 actgtttgga ctaggggccc atctcgggtt accgaattca gataaactcc gaatgccatt
      541 cattcatatc cgggagtcag actgtgagtg ataagatcca tagtcgaaag ggaaacagcc
      601 cagaccacca gctaaggtcc caaaatatat gttaagtgga aaaggatgtg gggttgcaca
      661 gacaactagg
//
LOCUS       DANKHK010000228          668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000228 DANKHK010000000
VERSION     DANKHK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(499..>668)
                     /locus_tag="PW109_002637"
     CDS             complement(499..>668)
                     /locus_tag="PW109_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2512385.1"
                     /translation="KYSRRKEQFQNEESMERFLVSSFDTYNQKFLGCSHKGFQQAEGE
                     LEQMLSQPMEN"
BASE COUNT          206 a          115 c          104 g          243 t
ORIGIN      
        1 agctttcttt gattcctcct ttttctttac tgaatttagt ataggataga tttcagttaa
       61 aaaaattgac gctcatcaag aaaagtattt cttaatagac gccaggcttc tctattaaat
      121 tcaactgaaa taatcaaagg aatgaagtcg ttccctccaa acaaaaattt cattaaagta
      181 gggcctaaaa aaaaatagca actatacttt gtcaagagac ctgactgcta tttttttgct
      241 tgttattctt caattaccaa atgtcttacc ttgcagtata tttaatactc tttctcacta
      301 gtgttgcatg caacactagt gagaaagagc attaataaga ttaacctaga agaataactt
      361 tactttattt atgtcatacc ataaataaaa attgaaaaga atttttttat ttatttttag
      421 tagatcgatt aataggagtt ttagcttggg agcgtcaata attttgtgta aatggttctt
      481 ccctactgta gattgtttct aattctccat cggttgactc agcatttgtt cgagctcgcc
      541 ttcggcctgt tggaaacctt tatgactgca acctaggaat ttttggttgt aagtatcaaa
      601 agacgagact aagaaacgtt ccatcgactc ttcattttga aactgctctt ttctgcggct
      661 gtatttct
//
LOCUS       DANKHK010000229          663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000229 DANKHK010000000
VERSION     DANKHK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            113..640
                     /locus_tag="PW109_002638"
     CDS             113..640
                     /locus_tag="PW109_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512386.1"
                     /translation="MTIIDTNKIEELLASKVSAYEIEKRSGITRNTISKLRTKKQEVM
                     KMSLESANKLQKFWEEYTMVKQYFDGSYDLFALEGNTQDRRRAVTNALHDFLKVEELS
                     EELENLVDGLRQEALNDSERLYLSFDNIAADAKEFDEIPTGGEMDNGKESVVLYKAVE
                     LESGKNLYILLTYDL"
BASE COUNT          258 a           80 c          126 g          199 t
ORIGIN      
        1 tcttatataa tcttaatacc tgaaagcgaa gcgtaacttt tagtttacgt aacttttaag
       61 ttacggtata attaatacat aaagaaaagc aaactaagaa gaggtttcta atatgacaat
      121 tattgacacg aataaaatcg aagaattatt ggcttctaaa gtttctgcat acgaaataga
      181 aaaaagatca ggtatcacta gaaatacgat aagtaaactg agaacgaaaa aacaagaagt
      241 aatgaaaatg agtttagaaa gtgcaaacaa attacaaaag ttttgggagg aatatacgat
      301 ggttaaacag tattttgatg gaagctatga tctttttgct ttggaaggta atacacaaga
      361 tcgacgccgt gcggttacga atgcgttaca tgattttttg aaggtagaag aattatctga
      421 agaactagaa aatctagtag atggcttacg tcaagaagct ttaaatgatt cagaacgtct
      481 ctatttatca tttgataata ttgcagctga tgcaaaagaa tttgatgaaa tcccaacggg
      541 tggcgaaatg gacaatggca aagaaagtgt tgttctttat aaagcagtag aattggaatc
      601 tggaaaaaat ttatatattt tactgaccta tgacttgtaa aacaaataag ttctttcttt
      661 tat
//
LOCUS       DANKHK010000230          625 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000230 DANKHK010000000
VERSION     DANKHK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..447)
                     /locus_tag="PW109_002639"
     CDS             complement(<1..447)
                     /locus_tag="PW109_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2512387.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          183 a          114 c           86 g          242 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatca
//
LOCUS       DANKHK010000231          573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000231 DANKHK010000000
VERSION     DANKHK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            126..>573
                     /locus_tag="PW109_002640"
     CDS             126..>573
                     /locus_tag="PW109_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDL2512388.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          207 a           87 c          120 g          159 t
ORIGIN      
        1 ttgcagcaaa atcatgttga taaagtgaca agaagactga tgagatatat tatccaaatt
       61 gagaagataa ttaatcggat tcttgagtat accctacttt ttaagaaagt agggtataca
      121 agcgtatgaa taaagacata actttccctg tgatggacgt tcggcttgtt ccaattacca
      181 aaatacaggc gaacgattat aatcccaatc gagtagcgtc tccggaaatg aacttattgg
      241 agctttctat cattgaagat ggctttactc aaccattagt ttgttattac aaggaaaaag
      301 aagagaagta tatcttggtt gatgtgcttc ataggtatcg tgttgctaag gaaagactca
      361 acttaaaagt tgtaccggta acagttattg ataaacccat agaagaacgt atggcttcaa
      421 caattcgaca caatcgtgca agagggaccc atgaaataag aggaatggaa attgtcatcc
      481 aaagattagt aaaaaatgga tggtcagaca agagaatatc caaagaatta ggcatggaca
      541 ttgaagaaat ttttcgcttt aagcaagtgt ctg
//
LOCUS       DANKHK010000232          516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000232 DANKHK010000000
VERSION     DANKHK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(118..417)
                     /locus_tag="PW109_002641"
     CDS             complement(118..417)
                     /locus_tag="PW109_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2512389.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          161 a           99 c           61 g          195 t
ORIGIN      
        1 aaacatctgt ttctttattt agaataaaaa aatacagact ctgttcaaat aaatgaacct
       61 agcctgcatt atgagattga ctatactttt taattcattg agatgaataa ctcgttttta
      121 taaatgccca aaagtaatcg gtgccgaagc cattcctcga cctaattctt tttcaaattt
      181 tcctattgtt gatatctcca tataaaacgg tgatactggt ttagacaatt ctcccctgat
      241 tgctaacgga cgtaaactac gttccaaatt atttatgact ttaggataat actgaccttt
      301 ttctaagcct gtctttgcgt ctatcaaaag tatacgttcc ttttctttga tctctggatt
      361 taaaacgaga tcatataatt ttagtaccat gttttctatt tcttcacttt tagacatcac
      421 gatttcctcc caaaaatata tttcttccca ctacattcta taaacaaagt atacgctaca
      481 tctagatttt tttaaatcat tactggattg ttaaaa
//
LOCUS       DANKHK010000233          515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000233 DANKHK010000000
VERSION     DANKHK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(346..432)
                     /locus_tag="PW109_002642"
                     /pseudo
     CDS             complement(346..432)
                     /locus_tag="PW109_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          171 a           97 c           83 g          164 t
ORIGIN      
        1 tgtatatgtc atttaggtat ttcagttatg ttataatagt tttgctgtct gctaccccgg
       61 cctaatctgg caaggggagg aggagatata atgaaatacc tttttgattt tgtttttgca
      121 atcaagccat ttgcagaggt aactgcacac tacatctgca agtggcttga ttgcaaaatt
      181 gccaaaaaag acagtaagcc tacccgttaa ttctataata tagaaacgta aaaaaaaccc
      241 catcagcctg cacgctgatg gggtttggtg atataatgaa atacctcttc gttactcata
      301 attataccat aattagtcac gaatgcaacc aatagaaaaa actacttaga acggtagatc
      361 atcgctaata acaatgcatt gtccaccaaa tggattttgt gaagcattaa atacattgtt
      421 attaattgtc gctgctttta actaggcctc tccccttcta ttataaaaga agatatatat
      481 catttcttaa ttacctacta ccttaaacca aaatt
//
LOCUS       DANKHK010000234          435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000234 DANKHK010000000
VERSION     DANKHK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            20..>435
                     /locus_tag="PW109_002643"
     CDS             20..>435
                     /locus_tag="PW109_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2512390.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIT"
BASE COUNT          148 a           69 c           92 g          126 t
ORIGIN      
        1 gatagcagga ggaatgacga tgaatcattt taaaggaaag caatttcagc aggatgtgat
       61 tattgtagcc gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat
      121 cttatatgat cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata
      181 tggcaaacta ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa
      241 aatggatgaa acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga
      301 tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta
      361 tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa
      421 agccccctct attac
//
LOCUS       DANKHK010000235          392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000235 DANKHK010000000
VERSION     DANKHK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
BASE COUNT          165 a           39 c           64 g          124 t
ORIGIN      
        1 tgaacgaaaa aacaataaaa tgctgtaata ttcgtgttta aaaataataa ataaaaatag
       61 ggaattttct attgcaattt tataaagaga ttgctatatt attatttgtt ctaaatgatt
      121 cctttaaggt gtcactaaaa attataaaaa atagtgttga cgcacttttg atgtcatgtt
      181 ataataacga agtcgtttgg atgagatagc taataaagct tgattttatc gaaaaaaact
      241 tcgaaaaagt tattgacaaa taacaataaa gatggtatga taatcaagtc gctgctaatg
      301 agcggcaaag acgtttagaa aaaataaaca aagaagttgt tgacattctc ttctgaatct
      361 gataagatta aagagttgtt aaaacaaaaa ga
//
LOCUS       DANKHK010000236          361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000236 DANKHK010000000
VERSION     DANKHK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..346
                     /locus_tag="PW109_002644"
     CDS             <1..346
                     /locus_tag="PW109_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2512391.1"
                     /translation="ANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPS
                     IRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          140 a           67 c           53 g          101 t
ORIGIN      
        1 agctaactca aaagaagaag cgaatacctt cttatccggc atgattgaga aatggaagaa
       61 aaactatcct aaagtgacgc agtcactcat agaaaaccaa gacttattaa ctttttatga
      121 ttttccacct agcattcgta gaaccattta ctcaaccaat ctaatcgagt ctttcaataa
      181 gcaaattaaa agatacagcc gtagaaaaga gcagtttcaa aatgaagaat cactagaacg
      241 ctttctagtc agcatttttg atacatacaa tcaaaaattt ctaaacagaa gccataaagg
      301 ttttcaacag gtaaccgata cattagtttc aatgtttact gagtaactaa ttattttgca
      361 g
//
LOCUS       DANKHK010000237          355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000237 DANKHK010000000
VERSION     DANKHK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            47..>355
                     /locus_tag="PW109_002645"
     CDS             47..>355
                     /locus_tag="PW109_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2512392.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     Y"
BASE COUNT          137 a           45 c           65 g          108 t
ORIGIN      
        1 ttagacttac aataagcagt gaatttataa tcaacgtata ggagacatgg atgaaatgaa
       61 actaattggt atagatttag acggtactct tttgaattct gaccagagca ttagtcaaaa
      121 aaatgcaaga acaatgaaac atttaccgga agactgtttt ccattcattt gttctggaag
      181 agaagtagaa gacattaata atattctcaa aaaatctggt ttaaggatac cagcagtagg
      241 gttaaatggt tctgttggat ttgatggaga aaaaaagata tttgagtctt ccttcgataa
      301 aaatgatgta aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa tatat
//
LOCUS       DANKHK010000238         8089 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.100, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000238 DANKHK010000000
VERSION     DANKHK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(134..577)
                     /locus_tag="PW109_002646"
     CDS             complement(134..577)
                     /locus_tag="PW109_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2512393.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PW109_002647"
     CDS             complement(795..2135)
                     /locus_tag="PW109_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2512394.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIILALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PW109_002648"
     CDS             2404..3300
                     /locus_tag="PW109_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2512395.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGMKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PW109_002649"
     CDS             complement(3450..4391)
                     /locus_tag="PW109_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2512396.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4516..5637
                     /locus_tag="PW109_002650"
     CDS             4516..5637
                     /locus_tag="PW109_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2512397.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5895..7397
                     /gene="eat(A)"
                     /locus_tag="PW109_002651"
     CDS             5895..7397
                     /gene="eat(A)"
                     /locus_tag="PW109_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2512398.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWELGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFVYFPQQTKDKERLTYYVLNDITDFEIWEIERELQF
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7425..7991
                     /locus_tag="PW109_002652"
     CDS             7425..7991
                     /locus_tag="PW109_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2512399.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2671 a         1502 c         1604 g         2312 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggtgttccag
       61 aagtagcttc ttcttatttc ttgaagctga tcacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg gatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgt caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gttccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 ataataacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat ggctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgattagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccgccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa gcgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttaat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggatgaa acataatagt
     2761 tcattttatt cgaaacatat ggatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttgcttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ctatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc ttttaagcca
     3181 atcccatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaataaataa
     3301 aaaacctaca ttttgaagag attatgtacc gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttc attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc taatatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gttttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cattatggct atagatcgga tttaattttg cttcttgaaa gacttccaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacgaaa catatcatct
     4141 aattgattcg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtgt gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc tccagctatt
     4381 gcgattttca tgttttttct ctcctttatc aatgagttcc cttttagtat atagaaaaat
     4441 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     4501 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     4561 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     4621 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4681 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
     4741 ttattgacaa cttggcaagg aaaaaatacc gcagtcatcg aatctgcaca agcattgggg
     4801 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     4861 ggagaactta tcaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     4921 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     4981 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     5041 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     5101 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     5161 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     5221 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     5281 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     5341 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     5401 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aatcatcgct
     5461 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
     5521 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     5581 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     5641 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5701 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5761 gggagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     5821 gtgtacccat cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     5881 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     5941 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg gaactaggac
     6001 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     6061 cttatcaagg gcaagtaatc catcagcaag aatttgtcta tttcccgcaa cagacaaaag
     6121 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     6181 tcgaaagaga gctccaattt atgcaaacag atcctgaaat cttatggaga gaattcagca
     6241 cactatcggg gggagagaag acaaaagtcc tattggcact tttatttgtg gatgacactc
     6301 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     6361 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     6421 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     6481 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     6541 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     6601 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     6661 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     6721 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     6781 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     6841 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     6901 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     6961 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     7021 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     7081 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     7141 tggaaagaga tgtttttcat aacaagattg agcagatgag tatgggccaa cggaaaaaag
     7201 tggaattggc taaatcttta tcacagccag ctgaactata tatatgggat gaaccattga
     7261 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     7321 ccatgttact ggtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     7381 ctcttgagaa aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     7441 ttgccaacga aggagaaaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7501 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     7561 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7621 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     7681 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     7741 gcaaagatga gaactttcaa aaagcacgtt tagcattcgc tgtacgattc ccaaatgggg
     7801 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc attgaagacg
     7861 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     7921 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     7981 cttatgagta attatgtgtt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     8041 ttagcagtgc ctttttttag gttatgacaa ttcttttggt aatataaac
//
LOCUS       DANKHK010000239         8014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.101, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000239 DANKHK010000000
VERSION     DANKHK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..709
                     /locus_tag="PW109_002653"
     CDS             <1..709
                     /locus_tag="PW109_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2512400.1"
                     /translation="LFDSRFKTLQQNRVKYFSELLASSERLSVDLESVIQSYNYGGGF
                     LGYVANRGNKYTFELAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQ
                     YLVTTEFDDDTVQAIMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRT
                     AQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADL
                     TSPYWQQHLVGAGRIKQ"
     gene            706..1638
                     /locus_tag="PW109_002654"
     CDS             706..1638
                     /locus_tag="PW109_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2512401.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            1883..1999
                     /locus_tag="PW109_002655"
     CDS             1883..1999
                     /locus_tag="PW109_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2512402.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2015..3934
                     /gene="tet(M)"
                     /locus_tag="PW109_002656"
     CDS             2015..3934
                     /gene="tet(M)"
                     /locus_tag="PW109_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2512403.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4050..4094
                     /locus_tag="PW109_002657"
                     /pseudo
     CDS             4050..4094
                     /locus_tag="PW109_002657"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            4128..5504
                     /gene="tet(L)"
                     /locus_tag="PW109_002658"
     CDS             4128..5504
                     /gene="tet(L)"
                     /locus_tag="PW109_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDL2512404.1"
                     /translation="MNTSFSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANYF
                     NKTPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSLIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVIVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWYYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            6068..7330
                     /locus_tag="PW109_002659"
                     /pseudo
     CDS             6068..7330
                     /locus_tag="PW109_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            7575..7973
                     /locus_tag="PW109_002660"
                     /pseudo
     CDS             7575..7973
                     /locus_tag="PW109_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
BASE COUNT         2719 a         1217 c         1650 g         2428 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt gtgaagtatt tcagtgaatt
       61 attagccagt agcgaaaggc tcagtgtaga tttagaatcg gttatccagt cctacaatta
      121 tggtggtggt ttcttagggt atgtggctaa tcgtggaaat aaatatacct ttgaactggc
      181 tcaaagtttc tcaaaagagt attcaggtgg cgaaaaagtg tcttacccca atcccatagc
      241 catacctatc aatgggggct ggcgatacaa ctatggcaat atgttttatg tgcaactggt
      301 aacgcagtat cttgtcacaa cagagtttga tgatgatacg gtacaagcca tcatggacga
      361 agcactgaaa tatgagggct ggcgatacgt ttacggtgga gcttccccga ctacttcttt
      421 tgattgtagc ggactgacac aatggacgta tggaaaagct ggaattaact taccacgaac
      481 cgcacaacag caatatgatg tgacccagca tatcccacta tcggaagcac aagctggcga
      541 tttggttttc tttcattcta cctataacgc tggctcttat attactcatg ttgggatata
      601 ccttggcaat aaccgtatgt ttcatgcagg cgacccaatc ggttatgccg acttaacaag
      661 cccctactgg caacagcatt tagtgggagc aggacgaatc aaacaatgag aaaggaagat
      721 ttaatgatga aatttagaaa aaatcagaat aaagaaaaac agataccaaa ggaaaagaaa
      781 cctcgtgtct ataaggtcaa tcctcataaa aaggttgtga ttgccttgtg ggtactttta
      841 gggcttagtt tcagctttgc gatattcaag cactttacag ctatagatac tcatactatt
      901 cacgaaacaa ctatcataga aaaggaatac gttgatactc atcatgtaga aaattttgta
      961 gagaactttg cgaaagtcta ctattcatgg gagcaatccg ataagtccat tgataatcga
     1021 atggaaagtc taaaaggcta tctgacagat gaacttcaag ctctcaatgt tgatacagta
     1081 cgcaaagata ttcctgtatc gtcttctgta agaggatttc agatatggac ggtagagcca
     1141 actggcgaca atgagtttaa tgtaacctac agtgtagacc agctcattac agagggagaa
     1201 aatacaaaga ccgtccactc tgcttatata gtgagtgtct atgtagatgg ttctggaaat
     1261 atggtactgg ttaagaatcc gaccattacc aacataccta agaaatcaag ttataaacca
     1321 aaagccattg aaagtgaggg gacggttgat tccattacaa ccaatgaaat caatgagttt
     1381 ttaacgacgt tcttcaagct ctatcctaca gcgacagcca gtgaactttc ctactatgtg
     1441 aatgacggga tattaaaacc aatcggaaaa gagtacatct ttcaagaact ggtaaatcct
     1501 attcacaatc gtaaggataa tcaagtcacg gtatcgctga cagtggagta tatcgaccag
     1561 cagaccaaag caacgcaggt atctcaattt gatttggtac ttgaaaagaa cgggagtaat
     1621 tggaagattg taaaataaca aatattggta catgattaca gatactttgt aatcatgtac
     1681 tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa
     1741 gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat
     1801 ttgatagcag gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc
     1861 agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg
     1921 tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta
     1981 tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
     2041 agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc
     2101 gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga
     2161 acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa
     2221 ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt
     2281 atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac
     2341 tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa
     2401 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
     2461 tatggaaatt ataatcaaac agaaagtaga gctgcaccct aatatgtgtg tgatgagctg
     2521 tacggaacct gagcaatggg atgtggtaat agaaggaaat gatgaccttt tagagaaata
     2581 tatgtccggt aaatcattag aagcattaga actcgaacaa gaggaaatca gaagatttca
     2641 gaattgctcc ttgtaccctg tttatcatgg aagcgcaaaa agcaacatag ggattgagca
     2701 gcttatagaa gtgataacga ataaatttta ttcatcaaca tacagaaaga agtctgaact
     2761 ttgcggaaat gtcttcaaaa ttgaatattc ggaagaaaga caacgtcttg catatgtacg
     2821 cctttatggc ggaatcctgc atttgcggga ttcggttaga atatcggaaa aggaaaaaat
     2881 aaaaattaca gaaatgtata cttcaataaa tggtgaatta tgtaaaattg ataaggctta
     2941 ttccggggaa attgttattt tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga
     3001 tacaaagcta ttgccacaga gagagagaat tgaaaatccg ctccctctgc tgcaaacaac
     3061 tgttgaaccg agcaaacctc aacaaaggga aatgttactt gatgcacttt tagaaatctc
     3121 cgacagtgac ccgcttctac aatattatgt ggattctacg acacatgaaa ttatactttc
     3181 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
     3241 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
     3301 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
     3361 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     3421 aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt
     3481 atatggttgg aatgtgacgg attgtaaaat ctgttttaag tatggcttat actatagccc
     3541 tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa
     3601 aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga
     3661 atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca
     3721 attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata
     3781 tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg
     3841 gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga
     3901 taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat
     3961 ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg
     4021 aggaattaat tatgaagtgt aattaatgta acagggttca attaaaagag ggaagcgtat
     4081 cattaaccct ataaactacg tctgccctca ttattggagg ttgaaatgtg aatacatcct
     4141 tttcacaatc gaatttacga cacaaccaaa ttttaatttg gctttgcatt ttatcttttt
     4201 ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca aattatttta
     4261 ataaaacacc tgcgagtaca aactgggtga acacagcctt tatgttaacc ttttccattg
     4321 gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta ctcctatttg
     4381 gaattataat aaattgtttc gggtcgttaa ttgggtttgt tggccattct ttcttttcct
     4441 tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca gcactcgtaa
     4501 tggttatagt tgcgcgctat attccaaagg aaaatagggg taaagcattt ggtcttattg
     4561 gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg atagcccatt
     4621 atattcattg gtactatctt ctactcattc ctatgataac aattatcact gttccgtttc
     4681 ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc aaaggaatta
     4741 tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat agcatttctt
     4801 ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg aaagtaacag
     4861 atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga gttctttgtg
     4921 ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat atgatgaaag
     4981 atgtttacca gctaagtact gccgaaatcg gaagtgtaat tattttccct ggaacaatga
     5041 gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt cctttatacg
     5101 tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc tttcttttag
     5161 aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg ctttcgttca
     5221 ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa gctggtgctg
     5281 gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt gcaattgtag
     5341 gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa gttgatcagt
     5401 caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc attagttggc
     5461 tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt aagggatcaa
     5521 ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa tctttttgtt
     5581 ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt atctttttta
     5641 ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat tttattcttg
     5701 ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg caagggttta
     5761 aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt gctgattttt
     5821 aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg tttttttgtt
     5881 tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg tcggcactgc
     5941 cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta tactttactt
     6001 ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga
     6061 tttacatatg agttatgcag tttgtagaat gcaaaaagtg aaatcagctg gactaaaagg
     6121 catgcaattt cataatcaaa gagagcgaaa aagtagaacg aatgatgata ttgaccatga
     6181 gcgaacacgt gaaaattatg atttgaaaaa tgataaaaat attgattaca acgaacgtgt
     6241 caaagaaatt attgaatcac aaaaaacagg tacaagaaaa acgaggaaag atgctgttct
     6301 tgtaaatgag ttgctagtaa catctgaccg agattttttt gagcaactgg atccaggaga
     6361 ataaaaacga ttttttgagg aaagttataa attattttcc gaacgatatg gcaagcaaaa
     6421 tattgcttat gcaacagttc ataatgatga gcaaacccct cacatgcatt taggtgttgt
     6481 gcctatgcgt gatggaaaac tgcaaggaaa aaatgtgttt aatcgtcaag aactgttatg
     6541 gctacaagat aaattccccg agcatatgaa aaaacagggt tttgagttga agcgtggtga
     6601 acgtggctct gaccgtaaac atattgagac agctaaattt aaaaaacaaa ctttggaaaa
     6661 agagattgat tttctagaaa aaaatttagc agttaaaaaa gatgaatgga ctgcttatag
     6721 cgataaagtt aaatcagatt tagaagtacc agcgaaacga cacatgaaaa gtgttgaagt
     6781 gccaacgggt gaaaagtcca tgtttggttt gggaaaagaa ataatgaaaa cagaaaagaa
     6841 accaaccaaa aatgttgtta tatcggagcg tgattataaa aacttagtga ctgctgcgag
     6901 agataacgat aggttaaaac agcatgttag aaatctcatg agtactgata tggcgagaga
     6961 atataaaaaa ttaagtaaag aacatgggca agttaaagaa aaatatagtg gtcttgtaga
     7021 gcgatttaat gaaaatgtaa atgattataa tgagttgctt gaagaaaaca agtctttaaa
     7081 gtctaaaata agcgatttaa agcgtgatgt gagtttaatc tatgaaagca ctaaggaatt
     7141 ccttaaggaa cgtacagacg gcttaaaagc ctttaaaaac gtttttaagg ggtttgtaga
     7201 caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac ctaaaaagaa
     7261 cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag atcagggaat
     7321 gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt tttgaacttg
     7381 ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt gctgaaaggt
     7441 gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa attggttgca
     7501 cagaaaaacc ccatctgtta aagttataag tgaccaaaca aataactaaa tagatggggg
     7561 tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa tcaagggttt
     7621 tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa atcgctaatg
     7681 ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga gtaaaagatt
     7741 gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag ttgtatcgag
     7801 tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca atgaaacatg
     7861 gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca gttcgttggt
     7921 tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat aagagggttc
     7981 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DANKHK010000240         7959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.102, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000240 DANKHK010000000
VERSION     DANKHK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            128..1564
                     /locus_tag="PW109_002661"
     CDS             128..1564
                     /locus_tag="PW109_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2512405.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PW109_002662"
     CDS             1753..4200
                     /locus_tag="PW109_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2512406.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PW109_002663"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PW109_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2512407.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PW109_002664"
     CDS             complement(5690..6793)
                     /locus_tag="PW109_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2512408.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="PW109_002665"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="PW109_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2512409.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DANKHK010000241         5754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.118, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000241 DANKHK010000000
VERSION     DANKHK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            complement(<1..2262)
                     /locus_tag="PW109_002666"
     CDS             complement(<1..2262)
                     /locus_tag="PW109_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2512410.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFF"
     gene            2685..3182
                     /gene="dfrG"
                     /locus_tag="PW109_002667"
     CDS             2685..3182
                     /gene="dfrG"
                     /locus_tag="PW109_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2512411.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(3254..5206)
                     /locus_tag="PW109_002668"
     CDS             complement(3254..5206)
                     /locus_tag="PW109_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2512412.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(5213..5449)
                     /locus_tag="PW109_002669"
     CDS             complement(5213..5449)
                     /locus_tag="PW109_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512413.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1711 a         1087 c          980 g         1976 t
ORIGIN      
        1 aaaaaagtta ccgctagcac agaaaagaat gatctgactt ctgaatttat tcagcggtga
       61 acagatttta tagagaacga ggaaccctac tgcaatcaaa agcatggtag atgctgtaga
      121 aacatctgtt ttatttagat tcaaagtcac cccgcagatc accaacgcac cgactacgat
      181 catatctgtt agtccacctg gcacagcttt ttccatcaca ttcatgatga atttccctct
      241 gattcgcttt ttgttttctt ctagagcaag caagaaagat ggaagtccga tagtaaacaa
      301 actaatcaat gtgatttgtg atggttctaa aggataggtc agagcaaaga tcactgaaaa
      361 cagtgagagc aaaaaggaga aaatattctt cactaaaaac aagctggctg atcgctcaat
      421 attgttcaca acgcgccgtc cttctgcaac aacttctggc atgcgtgaaa agtcagaatc
      481 aagtaagacc acttgtgagg cctgcatggc ggcttcattt ccggaagcca tcgctatact
      541 gcagtctgct tctttcatgg ctagaatatc attcacgcca tcacctgtca tagctactgt
      601 attgttacga cgctttagca attgaacgaa gattttcttt tgttctggct tgacgcgtcc
      661 aaaaactgta tatttttcga cagctgcttc atattcttct tctgataatt tagatgcatc
      721 aacgtactga tcagcatgtg aaatccctgc ttggacagct acattggata cggtgagcgg
      781 attatctcca gaaatcactt tgatggctac ttgctgtttt ttgaagtaat caaatgtggc
      841 tcttgcttct tttctgatag gattagcaat cagaatatag cctaatggtg tgactgcttc
      901 tgtcaatact ggttcttcaa gaatcccatc gtacacacca aagaccaaca cgcgatagcc
      961 tttttcagcg aaagaaacaa actcttccgc atattccggc aattgatcgc gcaaaaccat
     1021 ttcgggcgct cctaaaacat acaccttgtc tgccaacgct acactgctga atttttggac
     1081 agaagaaaaa ggaataacat ggtgatagga ttgatcagtg gtttttgtaa aatatgcttt
     1141 aacagcttcc attgttgcat tgtctcctgc catcgctttt gcatagtcag caatgcatgc
     1201 ttccaattga tctgactgac tgattatttc ttgtttttta gagacgatca ctttttgaac
     1261 agacatttta ttttctgtga tcgttcctgt tttatctaca caaaggacat ttacccttgc
     1321 caatgtctca atgcttttca tattgtgtaa aagaactctt tgctttgcta gtttagtcgc
     1381 acttaatgct aatgcaatcg tcgtcagcaa gtacagtcct tcaggtatca taccgatcaa
     1441 tgctgcttcc attgcaacga cactttcttt gatcgtgttt ccattaaaaa agtaactttg
     1501 agaaaaaagc aggataccga taggaataat gatgatccca atccatttga tcaattgatt
     1561 aagagaagca atcatctccg actgttctcc ttctcccatt gtcttggctt tgactgtcag
     1621 ctgggaaata tacgaatctg ctccaacttt gtctaatcgg gcgtaagctt ttccggaaac
     1681 aatgaaactg ccggacatta atggatcgcc tatttctttg gttatttcat ctgcttcgcc
     1741 tgtcaataaa gcctcattaa cttgcagttt cccttccacg acctctgcat ctgctggaat
     1801 ttgatgtcca gtctctaaaa taacaatgtc atctattaca aggtcttcta tatctattgt
     1861 ttgttctatc ccgtctcgta tgacattgac ttttgctgta tttaaaacat ttagttcgtc
     1921 caagatcttt ttggatcgaa tttcctgaaa aatggcgatt cctgtattgg ctatgataac
     1981 tggtaaaaaa gtcaagttct tataggattc tactaaacaa agcaaaatgg ctaagataag
     2041 aaagatcaaa ttgaaatacg taaatatgtt ttccaatacg atttgctgat ttgttttgaa
     2101 tgtgggatcg actgaattat ttgctgcacc tttttctatt cgttccttga cttgatctgc
     2161 cgttaatcct tctttatacg atggaaaaag acggatgact ttatctgatg atctttgtgt
     2221 tactttcttt tttttacttc gatttatcca tttattcttc atgtggctac ctcgattgat
     2281 ttcgttttct ggatatttcc aaacttctcc tatccagtat agaacagata tggaaagtgt
     2341 tcctcacatt aagcttgcaa acacctttca ttcctgtcat atagcaaaaa aaagctacga
     2401 cagttctatg taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg
     2461 actacttttt tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca
     2521 attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta
     2581 tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa
     2641 ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga
     2701 ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc
     2761 ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga
     2821 agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag
     2881 ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt
     2941 tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt
     3001 tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt
     3061 tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga
     3121 atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt
     3181 aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc
     3241 caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa
     3301 tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa
     3361 agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag
     3421 cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata
     3481 tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca
     3541 tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa
     3601 atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc
     3661 tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta
     3721 gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat
     3781 cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct
     3841 tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc
     3901 tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat
     3961 taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta
     4021 aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc
     4081 tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat
     4141 ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt
     4201 catccattga aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga
     4261 cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct
     4321 ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa
     4381 gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact
     4441 ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact ccaattccta
     4501 aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa
     4561 gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag
     4621 tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt
     4681 tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat
     4741 gaaattgata atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt
     4801 cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc
     4861 caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca
     4921 ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat
     4981 tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt
     5041 taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta
     5101 ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat
     5161 ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca
     5221 ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc
     5281 attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt
     5341 cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt
     5401 cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca
     5461 cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa
     5521 ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca
     5581 acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat
     5641 ttaggtttac attttcactt tttgtactcc aaaactgaac tacttaagtt ctatgtcgta
     5701 gctctttttt tcgaataaac ggtgttcaat cagctgaccc tcggtattag ctca
//
LOCUS       DANKHK010000242         5606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.124, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000242 DANKHK010000000
VERSION     DANKHK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            125..589
                     /locus_tag="PW109_002670"
     CDS             125..589
                     /locus_tag="PW109_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2512414.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            573..1202
                     /locus_tag="PW109_002671"
     CDS             573..1202
                     /locus_tag="PW109_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2512415.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1556..3034
                     /gene="msr(C)"
                     /locus_tag="PW109_002672"
     CDS             1556..3034
                     /gene="msr(C)"
                     /locus_tag="PW109_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2512416.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3081..3527
                     /locus_tag="PW109_002673"
     CDS             3081..3527
                     /locus_tag="PW109_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2512417.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3726..4241)
                     /locus_tag="PW109_002674"
     CDS             complement(3726..4241)
                     /locus_tag="PW109_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2512418.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4358..5560
                     /locus_tag="PW109_002675"
     CDS             4358..5560
                     /locus_tag="PW109_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512419.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1742 a          939 c         1198 g         1727 t
ORIGIN      
        1 ctctatactt ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt
       61 ctttttttat tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga
      121 aattatgtct ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa
      181 aatcgtccag atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat
      241 gagactgaaa gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga
      301 tcaaggagca gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca
      361 gagaaatcct cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa
      421 aatcaagcat tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag
      481 cgatgtagaa atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa
      541 tataacaaca gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa
      601 ttatgactgg tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaag
      661 aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat
      721 ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt
      781 tcaatacgag acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat
      841 gttacgaata tggcgaagaa atctacaacc aagcttacga atcagggaag atgccaaaag
      901 aagtcagaga tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag
      961 aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg
     1021 cgcgtgatca tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag
     1081 aggcatttgg tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag
     1141 cggtagaaga aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat
     1201 aaaaggtttt cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct
     1261 aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa
     1321 taacaactaa ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc
     1381 cattttttcg aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat
     1441 agttatttcg ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct
     1501 gctctttttt agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga
     1561 aaatttagca gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc
     1621 tattgattct cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt
     1681 cggtaaatcg actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat
     1741 ccaaacagag atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga
     1801 cctaaatttg gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa
     1861 atggagcgga ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca
     1921 agggatgctt ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat
     1981 tgaagagctt cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct
     2041 agatgagcta gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg
     2101 gaattatagt gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc
     2161 agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca
     2221 agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg
     2281 gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac
     2341 attagagaga agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat
     2401 ccgttttcca gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa
     2461 tgtccaattg gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt
     2521 aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca
     2581 tatccgcaaa caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca
     2641 gcaaaagggt tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac
     2701 agagttttca gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac
     2761 tttgacgaaa ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt
     2821 gctttttact aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat
     2881 ggcaacgatc gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac
     2941 ttctcatgat tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca
     3001 gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa
     3061 atttaaagga gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa
     3121 tgatgtgttt tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc
     3181 ttcatttatc aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg
     3241 atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag
     3301 aaagcaaggt ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat
     3361 gtatgtcttt attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt
     3421 atatgaatgc ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg
     3481 gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta
     3541 tttgaaaaaa ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa
     3601 tagtgatccg aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt
     3661 attgctcttt tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt
     3721 atcccttact caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc
     3781 tgctgacaaa gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg
     3841 tctagcgaga ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt
     3901 tctctagtga atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt
     3961 tttgctagaa attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct
     4021 acaagagcac gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg
     4081 tccaaaacgg tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg
     4141 tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg
     4201 gataaaagca cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc
     4261 ttattttctt tattatatca cagtaaagtt tccacgtttt acatgtgcat attgacatgt
     4321 aaaataaatc atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat
     4381 agatatacaa gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc
     4441 agctacctgt atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat
     4501 tgctgcacag ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt
     4561 agtcactggt gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa
     4621 aacattgatg acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg
     4681 gaatatcgga atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt
     4741 ctctactgct ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat
     4801 gatgtatgaa gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt
     4861 gggcagtggt tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt
     4921 tatagcccta gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg
     4981 ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt
     5041 atataatttc ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc
     5101 tgaattagaa gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat
     5161 tttcgttgca ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt
     5221 tgtcttattc attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt
     5281 accgatcagc attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac
     5341 gattgctatg gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt
     5401 ccgctttttc ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg
     5461 tgaaagctat gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat
     5521 ttatcaccgt aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa
     5581 agctgtcctg catcaagtca taagaa
//
LOCUS       DANKHK010000243         5179 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.132, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000243 DANKHK010000000
VERSION     DANKHK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            430..573
                     /locus_tag="PW109_002676"
     CDS             430..573
                     /locus_tag="PW109_002676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512420.1"
                     /translation="MGIDFINVEFRKHVKVSGDLGTTGSVCPFSQKQKMETLPENFLP
                     PFI"
     gene            827..2254
                     /gene="fexA"
                     /locus_tag="PW109_002677"
     CDS             827..2254
                     /gene="fexA"
                     /locus_tag="PW109_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FexA"
                     /protein_id="HDL2512421.1"
                     /translation="MKKDSKSKEMIQSEKRGSTRLLMMVLSLSVLVGSITADSVNPVL
                     PLISKALEASKSQVSWIVSGIALVLAIGVPIYGRISDFFELRKLYIFAIMILASGSLL
                     CAIAPNLPLLVLGRMVQGAGMSAIPVLSVIAISKVFPQGKRGGALGIIAGSIGVGTAA
                     GPIFGGVVGQYLGWNALFWFTFLLAIMIVIGAYYALPTIKPAESVGSNKNFDFIGGLF
                     LGLTVGLLLFGITQGETSGFSSFSSLTSLIGSVVALVGFIWRIVTAENPFVPPVLFNN
                     KDYVNTVIIAFFSMFAYFAVLVFVPLLVIEVNGLSSGQAGMILLPGGVAVAILSPFVG
                     RLSDRFGDKRLIITGMTLMGLSTLFLSTYASGASPLLVSVGVLGVGIAFAFTNSPANN
                     AAVSALDADKVGVGMGIFQGALYLGAGTGAGMIGALLSARRDATEPINPLYILDAMSY
                     SDAFLAATGAILIALIAGLGLKKRG"
     gene            2440..2610
                     /locus_tag="PW109_002678"
     CDS             2440..2610
                     /locus_tag="PW109_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2512422.1"
                     /translation="MGDRFSPRYAKFLLVYTNQSISEISEYLIFNSQSYFTSVFKKET
                     GKTPFEYRKSDL"
     gene            2942..4909
                     /gene="optrA"
                     /locus_tag="PW109_002679"
     CDS             2942..4909
                     /gene="optrA"
                     /locus_tag="PW109_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein OptrA"
                     /protein_id="HDL2512423.1"
                     /translation="MSKATFAIASTNAKEDMEMQYKIINGAVYYDGNMVLENIGIEIN
                     DNEKIAIVGRNGCGKTTLLKAIIGEIELEEGTGESEFQVIKTGNPYISYLRQMPFEDE
                     SISMVDEVRTVFKTLIDMENKMKQLIDKMENQYDDKIINEYSDIFERYMALGGLTYQK
                     EYETMIRSMGFTEADDKKPISEFSGGQRTKIAFIKILLTKPDILLLDEPTNHLDIETI
                     QWLESYLRSYKSTLVIISHDRMFLNRIVDKVYEIEWGETKCYKGNYSAFEEQKRENHI
                     KQQKDYDLQQKEIERITRLIERFRYKPTKAKMVQSKIKLLQRMQILNAPDQYDTKTYM
                     SKFQPRISSSRQVLSVSELVIGYDTPLAKVNFNLERGQKLGIVGSNGIGKSTLLKTLM
                     DGVAALSGDFKFGYNVEIGYFDQQLAQISGDDTLFEIFQSEYPELNDTEVRTALGSFQ
                     FSGDDVFRPVSSLSGGEKVRLTLCKLLYKRPNVLILDEPTNHMDIIGKENLENILCSY
                     QGTIIFVSHDRYFTNKIADRLLVFDKDGVEFVQSTYGEYEKKRMNSEKPFNNIKVEQK
                     VEKNNTVKGDRNSIEKEKVKKEKRIEKLEVLINQYDEELERLNKIISEPNNSSDYIVL
                     TEIQKSIDDVKRCQGNYFNEWEQLMRELEVM"
BASE COUNT         1684 a          750 c         1071 g         1674 t
ORIGIN      
        1 gtaaattact aaaaatccat taacatcaat gtttttactt taagaacata tatttaacgt
       61 gttaaatcaa atgaaaaaat acaatgtaat tattagatga caattgatta cggtcagaag
      121 aatgtaccac aaataaaaaa actaattcct ttttatttta tcgaggaatt aagcttttga
      181 cctgcaacaa tcacgcccaa tttcggaata tctataatat gatttcgatc ttaaattatc
      241 tactttaact gacgctacct gctcgttaaa gaagacctat ttaaaaaagt ttatactgca
      301 cctgccttta ttatgtaagt aaaagtagta aagctttaaa ttggttttca aaaaagtagg
      361 gttgattatt tctttttcat caattataat tgtgttatta tcacaatatg agaaaggagg
      421 caaaaagtaa tgggaattga tttcattaat gtcgagttta gaaagcatgt aaaagtttca
      481 ggggatttag gtacaacggg atcagtatgt cctttttcac aaaaacaaaa aatggaaaca
      541 ctaccggaaa actttttgcc tccttttata taatgggcaa aaatttatga atctattgca
      601 tttgtaaaaa tgcatacgaa tgattaggca ccggttgtta aactggtgcc ttttttttgt
      661 ttgatcagtt cagttagttt ggagaaccga atctttaatc atgctgtaga aatatggtga
      721 gttcgttgat ttggttttac aatataaata gatatgaggt gaggtttatg gtgaaagcag
      781 tggatttagc ctcctaaaat cacaatatat tttaggagga aataaaatga aaaaggatag
      841 taaatctaaa gaaatgattc aatctgaaaa aaggggttct actaggcttt taatgatggt
      901 actctcccta tctgtacttg taggttcaat tacggctgat tcagtcaatc ccgtacttcc
      961 actaataagc aaagctttag aagcttcgaa atctcaagtg agttggatag ttagtggtat
     1021 tgcacttgtt cttgcgattg gagttccgat ttatggtcga atctcagact tttttgagtt
     1081 acgaaagcta tatatctttg ccattatgat tctggcaagt ggtagtcttt tatgtgcaat
     1141 tgccccgaac ctcccattgt tggttttggg aagaatggtt cagggtgctg ggatgtccgc
     1201 aattccagtt ctatcagtca ttgcaatttc gaaggttttc ccacaaggaa aacgtggggg
     1261 agctttggga attatcgcag gaagtattgg tgttggaact gctgctggtc caatatttgg
     1321 tggagtagtt ggtcaatatt tagggtggaa tgccttgttt tggttcacat ttttgttagc
     1381 cattatgatt gttattggtg cctactacgc gttaccgaca attaaaccgg cagaatccgt
     1441 aggaagcaat aagaactttg atttcattgg tggtttattc ctcggcctca cagtaggatt
     1501 actccttttt ggcatcactc aaggagaaac ttctggtttt tcttcgttct catcgttaac
     1561 tagcctaatt ggttctgttg tagctttggt gggatttatt tggagaattg ttaccgcaga
     1621 aaatccattt gtaccacctg tcctgttcaa taacaaggat tatgtcaata cggtcataat
     1681 tgcatttttt tcgatgtttg cttatttcgc tgttcttgtg ttcgtcccat tactagtcat
     1741 tgaggtgaat ggactctctt ctggacaggc tggaatgata ttgttgccag gtggtgtggc
     1801 tgttgcaatc ttatctccct tcgttggccg tctttctgat cgatttgggg ataaacgtct
     1861 gataattact gggatgactc tgatggggct gtctacctta ttcttgtcca cctatgcatc
     1921 tggtgcttca cctctgttag tttccgtggg ggtcctcgga gtagggattg cttttgcatt
     1981 cacgaattct cccgcaaata acgccgcagt aagtgcactc gatgcagaca aggttggtgt
     2041 cggaatgggg attttccaag gtgctttgta ccttggagca ggaactggag caggtatgat
     2101 tggagcatta ttatccgctc gacgtgatgc tactgagccg ataaatccat tatatatatt
     2161 ggacgctatg tcctactcag atgcgttcct tgcagctaca ggggcaatac tcattgcctt
     2221 aatagctgga ttaggtttaa aaaagcgtgg gtaataactt agttatgtaa agattagtta
     2281 ttgtttagtt catcttctat agtttaagct gtaaataaat agcttaggaa tcacgcaaat
     2341 ttgaatttct tagaatagaa tacttttaag taactcaact ttcgtacact gttttattgg
     2401 tgcgaaagtt gagtatgtta aggatgaaac aatgaaaaca ttggtgatcg tttctcaccc
     2461 agatatgcca aatttctatt ggtatatacg aatcagtcaa tatcagaaat tagtgaatat
     2521 cttatattta actcacaaag ttacttcaca tccgtgttta aaaaagaaac aggaaaaacg
     2581 ccttttgaat atagaaagag tgatttgtaa agctgatggc taatgcaaag aatcaattgc
     2641 ggcaatcctt ttgtatcttt ttccagtgtg gctgcggttt ctgttgacgg gaacagcagg
     2701 gtttatcggg aatctggtgc cagatgagat aacagatcag ttactttact tttaccagaa
     2761 agggtgtaag ctaaaactta ggctagccat tgcgctagcg acttggtaca ataaggtggc
     2821 aattgaatta tattaataat cgataggagg tagaagcaaa tgaaatctac aactatagta
     2881 acatttaaag aactccatgt ataggtggct gtgacaagct ttacatggag tagaataatt
     2941 attgtccaaa gccacctttg caattgctag tactaacgca aaggaggata tggaaatgca
     3001 atacaaaata attaatggtg ccgtttacta tgatggtaat atggtgttgg aaaacatcgg
     3061 tattgaaatc aatgataatg aaaagattgc tattgttggt agaaatggat gtggaaaaac
     3121 aaccttgcta aaagctatta taggcgaaat tgaattagaa gaaggaactg gtgaaagtga
     3181 gtttcaagta ataaagaccg gtaaccctta tattagctat ttaagacaga tgccttttga
     3241 agatgaaagt atatcaatgg tggatgaagt ccgtacggta tttaagacgc ttattgatat
     3301 ggaaaacaag atgaaacagc tgatagataa aatggagaat caatatgatg ataaaatcat
     3361 caatgaatac tctgatatct ttgaaaggta tatggctctt ggaggtctaa cctaccaaaa
     3421 agaatatgaa acgatgattc gtagtatggg ttttactgaa gcagatgata aaaaacccat
     3481 ttctgaattt tcaggtggtc agcgaactaa gatagctttt ataaaaatac ttttaacaaa
     3541 gccagacatt ctattacttg atgaacctac taaccacctt gatatagaaa caatacaatg
     3601 gttggagagt tatttgagaa gttataaatc tacattggtt attatttccc atgatagaat
     3661 gtttcttaat cgaattgtgg ataaggttta tgaaatcgaa tggggagaga ccaaatgtta
     3721 taaaggtaat tattcagcct ttgaggagca aaaacgagaa aatcatatca aacagcaaaa
     3781 agattacgac ttgcaacaga aagaaattga aaggattaca cgcttgattg aacgttttcg
     3841 ttataaacct acgaaagcta aaatggtgca atctaaaatt aaattattac agcgtatgca
     3901 aatattaaat gcaccagacc aatacgatac aaaaacttat atgtctaaat ttcaaccgag
     3961 aatcagtagt tcaaggcaag tattaagtgt ttcagaactt gtgataggct atgatactcc
     4021 tcttgcaaag gttaatttca accttgaaag gggacagaag cttggaattg ttgggagtaa
     4081 tggtattggt aaatccacgt tgcttaaaac acttatggat ggtgtggcag cattgtctgg
     4141 agattttaaa ttcggataca atgttgaaat tggctatttt gaccaacagc ttgctcaaat
     4201 cagtggagat gatacactat tcgaaatttt tcaaagcgaa taccctgagc taaatgacac
     4261 agaggtcaga actgctcttg gctcatttca gtttagtgga gatgatgttt ttagaccggt
     4321 gtcctctttg tcaggtggag aaaaggttag attgacatta tgtaaattat tatataaacg
     4381 tcctaatgtt ttaatcttag atgaaccgac aaaccacatg gatattattg gaaaagagaa
     4441 tttagagaat atcttatgca gttatcaagg tacaattatt tttgtgtcac atgatagata
     4501 ttttactaat aagattgctg acagattact tgtttttgat aaggatggtg tagagtttgt
     4561 acaatctact tatggtgagt acgagaaaaa aaggatgaat tctgaaaagc catttaataa
     4621 cattaaagtt gagcagaaag tagagaaaaa taacacagta aaaggcgatc gtaactccat
     4681 tgagaaggag aaggttaaga aggagaaacg aattgaaaag cttgaagtgt taataaatca
     4741 atatgatgaa gaattagaaa gattgaataa aatcatttct gaaccaaaca attcttctga
     4801 ttatatagta ctgacggaaa tacaaaaatc aattgatgat gttaaaaggt gtcagggtaa
     4861 ttattttaat gaatgggaac agttgatgag agaattggaa gttatgtaat ttttgagtta
     4921 tgcaataagg atgtaaaagt gttaatgata cattctggtt gttgaaaatg taaaaaaatt
     4981 aatgaatttt tattagatat aatcgctatg tgaagacctt ttgagtttgc cgcatagcgg
     5041 tttttattat ctatactttg gaattgtcaa gaaaagtgta gtcgtataaa ctcactaaat
     5101 ttcctttgat tgttatgctg ccagaagttg ttcttccagc tgaacaaaat tattacgatt
     5161 tatatgaaaa gttgttgcc
//
LOCUS       DANKHK010000244         3523 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.150, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000244 DANKHK010000000
VERSION     DANKHK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            44..1393
                     /locus_tag="PW109_002680"
                     /pseudo
     CDS             44..1393
                     /locus_tag="PW109_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
     gene            1790..3280
                     /gene="abc-f"
                     /locus_tag="PW109_002681"
     CDS             1790..3280
                     /gene="abc-f"
                     /locus_tag="PW109_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDL2512424.1"
                     /translation="MTTIEIKNLTFGFDVQTTNLFEQTNLTIQSEWKLGLIGRNGRGK
                     TTLLKLLMGEYTYSGKIEHQLNFTYFPQKVQDKTQLTYYVLQELSDFEQWKIERELNL
                     LQVDSDILWRPFHSLSGGEQTKVLLALLFVDDVNYPLIDEPTNHLDVMARQQVAAYLK
                     AKKQGFIVVSHDRSFVDEVVDHVLSIEKSQLVLYQGDFSVYEEQKGLRDQFEQEQNVK
                     LKKEIGRLKQTAAEKAEWSRGRERDKLGSPNKPGSGAIYDTGAIGARAARTMKRSKAI
                     VKRMEDQATEKEKLLKDMEYIEPLSMNYQVTHRKKLLTVQELQLSYGERKLFSPLTFD
                     INQGDRLAIQGPNGSGKSSVIQHLLGIFQGEVTGEVIQPQGVTISYVRQNYEDNRGTL
                     QEFAEEHHLSYQELLNNLWKLGVERKVFQNRIEEMSMGQRKRVELAKSLATPAELFIW
                     DEPLNYLDVFNHKQLEEVIQLVQPTMLIVEHDRTFLNKVATKIIPL"
     gene            complement(3357..3503)
                     /locus_tag="PW109_002682"
     CDS             complement(3357..3503)
                     /locus_tag="PW109_002682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512425.1"
                     /translation="MLDRLKEELEIPFLQIKLQDKTYSEEEYQQLKTELVQYFDDYVR
                     NVEN"
BASE COUNT         1220 a          564 c          757 g          982 t
ORIGIN      
        1 atataatggg agataagacg gttcgtgttc gtgctgactt acaatggtga tgttctttgc
       61 gcaattaatt caactgatgg ttcaattagc ttttcaacaa ttggatgaag aagtgtcttc
      121 agaattgaaa aaagaagggt tccaagttga tcgaaaaagc caacgaaccg ttacttttct
      181 atttggttct gtcacttatg tgagacgaag aatgaagaat aaagccggtg aaatccgcta
      241 tccccttgac gaatttttag ggatacgcaa aggaattcgc tatagttcac tggtcttaag
      301 aaatgtctct gaattaggta caatgatggt gtatcgtcac gtgtcaaaag cgattgattg
      361 cttaactacc tggagtatga gccatcaaaa cgtccaacaa ttggtggtga aaacaggaga
      421 aattgtgcag aaacgaagta cgcatgaaag tcgatatgac gaagtaaaac aaaagaaaaa
      481 agttccttac ttgtatctag aaggtgacgg tgtgaggatt cggggacaga aaaaacaaca
      541 cctagaaatt caccgttttc aagtgagtga aggtagccaa aagataggca aaagatccca
      601 gttaatttct ccttattttg tcagtcattt gaatcgaaaa aatgcccaaa aagagatgat
      661 gaattatctt caagcaacct atgatttaac agagacaatc gtgatatcca acagtgatgg
      721 tggttctggc tatgaaaaag aagtctttga ggaactttct ttgggctgtt tccgtcatga
      781 acattttcgt gacatgtatc atgtgcatcg aaaaataaaa gagcgactaa attttgtttc
      841 atctaagcta caaggaaaat taatagaagc gattcaccag tacgacaaag aaaaagtacg
      901 tgcttgtttg gacaccgcag aaagtctgat tgaggagcga gaagaaacct gtttggaaca
      961 gttgaggtta ttgcaaggct atcttgatcg gaattgggtg tatttacaat ctcttgaaaa
     1021 aagagggttc tatgacgata agcaaagtat cggaaccatt gaaagtacgc atcggaaaat
     1081 gacctatcgg atgaagcgac aaggtcgttt gtggagtaga acaggagcgg cagccatgat
     1141 tcgtgtgata gatagtttga ggaatcaaga attgaatact tggctaaatc aatatgaaga
     1201 agtggcggat gacgaattag aacgacgaaa acaatggaaa gcaatgaaac gaaaagtcct
     1261 aaaagcccct gcttttcaat cacatgaagg agcatttaaa gggagcattg gtcagggact
     1321 tgcgaaaagc gcaccaatag gtcaactatc aaaaggactg aagcaattaa acatgacccc
     1381 tagttatttc taatggaaaa aatttttcca ttagaaataa ctaggcaaac cgaacgcaag
     1441 tgaggtacta ataacaaaat ttaagtgaag tcagctgctt ttttattcaa cctcaaaaaa
     1501 tttgacacat acaaaatcaa gaaatcattt gctttctaat ccaatttatg ctattcttat
     1561 actaatcaaa aacgaagtga gggatcctct attgaaaaat taagccaatg tgttgaagct
     1621 gcattcagac tattttcttt gaagtacaag ggaatagtgt gcagattttc aggattggtg
     1681 attatttttc gataagaggg tctttttgtg tcttcctatc taaaaaatct ctgtcctgaa
     1741 aataggtgag ggatttttct tgcctaatcg gtgagtcgag ggaatttaaa tgacaacaat
     1801 tgaaataaag aatttaacgt ttggctttga tgtccaaaca acgaacttat ttgaacaaac
     1861 gaatctcacc attcaatcag aatggaaatt aggattaatt ggccgcaatg gtcgtggcaa
     1921 aacgacacta ctaaaattgt tgatgggtga atacacgtat agtgggaaaa ttgagcatca
     1981 gttgaacttt acttattttc cacaaaaagt tcaagataag acgcaattaa cgtattatgt
     2041 gctacaagaa ctctctgatt ttgaacaatg gaaaatagaa cgagaactca acctcctaca
     2101 agtagattca gatattttat ggcgaccgtt tcatagttta tcaggtggag agcaaaccaa
     2161 agtattgtta gccttgcttt ttgttgatga tgtgaattat ccattaattg atgaaccaac
     2221 caatcatttg gatgtaatgg ctcgtcaaca agtggcggcg tatttaaagg cgaagaaaca
     2281 agggtttatt gttgttagcc atgatcgaag ttttgtggat gaagtggtgg atcatgtatt
     2341 atccattgaa aaaagtcagc tagtgttgta tcaaggagat ttttcggtct acgaagaaca
     2401 aaaaggctta cgtgatcaat tcgaacaaga gcagaatgtg aaactcaaaa aagaaattgg
     2461 acgcttaaaa caaaccgcag cagaaaaagc agagtggtca cgtgggagag agcgagataa
     2521 attgggctcg cccaacaaac cgggaagtgg agccatttat gataccggtg cgattggtgc
     2581 gcgagctgcg cgaacgatga aacgctccaa agcaatcgtc aagcgtatgg aagatcaagc
     2641 aacagaaaaa gaaaaattat tgaaggatat ggaatacatt gagccattaa gtatgaacta
     2701 tcaagtaact catcgaaaaa aattactgac tgtgcaagag ttgcagttga gttatggaga
     2761 acgaaagcta ttttctcccc ttactttcga cattaatcaa ggggatcgat tggcaattca
     2821 aggaccaaat ggctcaggaa agtcttcagt tattcagcat cttttaggaa tatttcaagg
     2881 tgaagtgaca ggtgaagtca tacaaccaca aggagtaacc atcagttatg ttcgccaaaa
     2941 ttatgaagat aatcgaggaa ccttgcaaga atttgctgaa gaacatcacc tatcctatca
     3001 agaattattg aataatctct ggaagctggg agtggaacgg aaggtttttc aaaatcggat
     3061 tgaagagatg agtatggggc aacgaaaacg ggtagaatta gcaaaatctt tagcgacacc
     3121 agcagagcta tttatctggg atgaaccctt aaattatcta gatgtcttta accacaagca
     3181 gttggaggaa gtcattcaac tcgttcaacc aacgatgctg attgttgaac atgaccgaac
     3241 ctttttgaat aaagtagcga ctaaaattat cccgttatag taaaaaagta aaatagaaaa
     3301 tccttatgaa atttgtttta agaacatcaa tttcataagg attctttcat ttttgtttaa
     3361 ttttctacat tacgcacata atcatcaaag tattgaacta attcggtttt taattgttga
     3421 tattcttctt cggaatacgt tttgtcttgc agtttgatct gtaaaaaagg aatttctagt
     3481 tcttctttga ggcgatcaag cacatctaac tgatccattt ttt
//
LOCUS       DANKHK010000245         3398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.154, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000245 DANKHK010000000
VERSION     DANKHK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            301..1710
                     /locus_tag="PW109_002683"
     CDS             301..1710
                     /locus_tag="PW109_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512426.1"
                     /translation="MNHQNEKNIASNVLLISILGLSVLVGSVTADMVNPVLGVIGKEL
                     GGSEAQVSWVVSGVALVLSIAIPFYGRLSDFLNIKKLFTNGFLILTIGSLICIFAPNL
                     IILVLGRMFQGAGMAAIPALSIVIISKIYPPGQRGRILGIIAGCIGVGTAGGPIFGGV
                     VGQLLGWQSLFWVTFVLGLIIVLGVQISMPKIESPDNNSHQNFDVLGGLLLGLTVGGF
                     LLGITLSEMYGLISIQTTTSFSISMIALIVLIYRVINVKNPFIPPVILKNRLYVSSIF
                     IVFLSMFAYVSMLVFIPLLVVEVNGLSTGQAGLILLSGGVAVAILSPIVGRLSDKVHP
                     KILLLVGLIIMGLSSLYMSFVAGASPVLLSIGSLGIGIAFAFINSPVNNVAVLALPKE
                     QVGVGTGLFQGAMYLGAGTGASLIGALLSMRHGVKASFNPFYTLTAPHYSDIFLTITC
                     IVLVALIVTLNISSRDLKQ"
     gene            complement(2040..>3398)
                     /locus_tag="PW109_002684"
     CDS             complement(2040..>3398)
                     /locus_tag="PW109_002684"
                     /inference="COORDINATES: protein motif:HMM:NF037897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512427.1"
                     /translation="LFDSRFKTLQQNQWEDILKWGTVIVPETSSYISDNPIQELDDMG
                     FNSLPLNDNHIIKENTTTEKRSKIDNLYHGSKEDFTEFKIPRFHKNGGTLGFGTYLTD
                     SLQRAKSYADNDGYLYTIELNDELQNSTPLNASKVTLTTNQVAKIIEKIAQKQIDEEE
                     YPYILSDWEEPTSETAMDAGNKKIAYNIAEKIIDDTDDLSIINGINNQLGGNDSGAET
                     LIPVLNEMGIHYAELDVEYYDNSKEYVVFNPKDIKIVNQKLTKPEQSKKFVSDVDIKS
                     LEEKAIKTIAKSELIVKANNPVEAMQKLYNNDLKSAIKASFILYDLPEDKIDSSKKNV
                     TWEDLVNIEKSYNVFSYYQNNLYGSELVEPDLNNPYQENTKILDNKATQKQPALSDQY
                     KALHDKLKEPGANKQQIQEQLNKVKIEISQKTQQQLKDFAKANPDLKQPRVKKEQQAL
                     QR"
BASE COUNT          970 a          512 c          578 g         1338 t
ORIGIN      
        1 gtatagatta atataagagg aggataaaaa tgatggaaat ggtgtttacc ctttataaaa
       61 caattaaagt ttcagactat ctcaaagttg agggacaagt atgtactttt ttaaacagaa
      121 taccaaaatc cagacttttt actataatcc ttacgtaaaa aagtaaaata ttgatttatg
      181 taacaaaagt ctgatcttaa gattaggttt tttatttgga taattgagag gtgaaattga
      241 tggttaaagc agtcgattta gccttctaaa atgagcattt aaattttagg aggctgtaaa
      301 atgaatcatc aaaatgaaaa aaatatagca tcaaatgtgt tgttaattag tatcttaggc
      361 ttgtctgttt tagtgggttc agttactgca gatatggtta atcctgttct cggtgtgatt
      421 ggaaaagaat taggtgggtc tgaggcgcaa gttagttggg tcgtaagtgg tgttgcctta
      481 gttctatcta tagcaattcc cttttatgga cgtttatctg attttttaaa tattaaaaaa
      541 ctctttacta acggatttct aatattaact ataggaagtt taatatgtat attcgcgccc
      601 aatttaatta ttttagtatt gggtagaatg tttcaaggag ctggcatggc agccatacct
      661 gcgctatcta ttgttataat atctaaaatt tatccgcctg gacagagagg aagaatttta
      721 ggtattattg ctggttgtat tggcgttggc actgcaggag gcccaatatt tggcggcgtt
      781 gtaggacaat tattaggttg gcaatcatta ttttgggtca cttttgttct gggtttaatt
      841 atagttctag gtgttcaaat atcaatgcct aaaatagaat caccagataa taacagtcat
      901 caaaattttg atgttttagg tggactatta ttaggattaa ccgtaggtgg ttttttactt
      961 ggtattacgc tttcagaaat gtatggtttg atttctatac aaacaaccac aagtttttct
     1021 atatctatga tagctttaat agtgttaata tatcgcgtga ttaatgttaa aaatcctttt
     1081 atccctccag ttatattgaa gaaccgttta tatgtaagct caattttcat tgtatttctt
     1141 tcaatgtttg cttatgtctc tatgcttgtt tttattccat tattagttgt tgaggttaat
     1201 gggttaagta ctggacaggc aggcttaata ttactttctg gtggtgtcgc tgttgcaatc
     1261 ctttcaccaa tagtgggaag attatctgac aaagtgcatc ctaaaatact attattagtt
     1321 ggactaatta ttatgggctt atcttcttta tatatgagct tcgtagcagg cgcatcacct
     1381 gtattattat ctatcgggag tttagggata ggtatcgctt ttgcatttat taactctcca
     1441 gtgaataatg ttgcagtact tgctttacct aaagaacaag ttggtgtagg tacggggttg
     1501 tttcaaggtg caatgtatct tggggcagga acaggtgcct cactaatagg ggctttattg
     1561 tcaatgagac atggggttaa agcatctttt aatccttttt atactttaac tgccccgcac
     1621 tattctgata tatttttaac cattacgtgc atcgtgttag ttgctttaat tgttacttta
     1681 aatataagta gtagggattt aaagcaataa aaccttttta ttacacagat atacatctgt
     1741 ttttaaatat tattcagaaa ctaaattgtt aatagcaaga acaactaaat aattttaaaa
     1801 aacagggctt attaacaagc cctgttttgc attttagcca agaaaataac cagtttctgt
     1861 acactatcta actatcactt ttttcaattc ataatattgt atagcattcc atacgtttca
     1921 ttttctcatt tattaagtaa aatcattcta atttagcaag taaagtcaca acggtcttag
     1981 aatgcgcctc tcaaacgtca aaatacaaaa aagcgcctat aaataggcgc tttttatgat
     2041 tatcgttgca aagcttgctg ttcttttttt actcttggtt gcttcaaatc tgggttagct
     2101 tttgcaaagt cttttaattg ttgctgggtc ttttgactaa tttcgatttt aactttgttt
     2161 agttgttcct gtatttgttg tttatttgca cctggctctt ttaacttatc atgcaatgct
     2221 ttgtattgat ctgataaggc tggttgtttt tgtgtggctt tattatcaag aatcttagtg
     2281 ttttcctgat atggattgtt caaatcaggt tctacaagtt cactaccgta taagttgttt
     2341 tgataataac taaacacatt atatgatttt tctatgttga ctaaatcttc ccatgtaaca
     2401 ttctttttac tgctgtcgat tttatcttct ggtaaatcat ataatataaa gctggctttt
     2461 atagcgcttt ttaaatcatt gttataaagc ttttgcatag cttcaactgg gttgtttgct
     2521 ttaacaatta gttcagattt tgcgattgtt tttatagctt tttcttccaa ggattttata
     2581 tcaacatcac taacaaactt tttactttgt tctggttttg ttagtttctg attaacgatt
     2641 ttaatatcct ttggattaaa tacaacgtat tctttggaat tatcatagta ttcaacatct
     2701 aattctgcat agtgaatacc catttcattt aaaacaggaa ttagtgtttc tgctccacta
     2761 tcgttaccgc ctaattgatt gtttatgcca ttgataatgc ttaaatcatc agtatcatca
     2821 ataatctttt cagctatatt ataagcaatt tttttgttgc ctgcgtccat tgctgtttca
     2881 ctagttggtt cttcccaatc ggacaagatg taggggtatt cttcttcatc aatctgtttc
     2941 tgtgctatct tttcaattat tttagcgact tgattagttg ttaatgtaac tttgctggcg
     3001 ttcaaaggtg tactattttg tagttcatca ttcaattcaa tagtatacaa atagccgtca
     3061 ttatctgcat aagatttagc tctttgcaag ctatcagtta gataagttcc aaagcctaat
     3121 gttccaccat ttttatgaaa tcttggtatt ttaaattcag taaaatcctc ttttgaccca
     3181 tgatataaat tgtctatttt acttcgcttt tcagttgtag tattttcttt aatgatgtga
     3241 ttatcattta aaggtaagct attgaatccc atatcatcta attcttgaat tgggttatcg
     3301 ctaatgtagc tacttgtttc tggtacaata actgtccccc attttaaaat atcttcccat
     3361 tggttctgtt gcaaagtttt aaatctacta tcaaataa
//
LOCUS       DANKHK010000246         3398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.155, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000246 DANKHK010000000
VERSION     DANKHK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            301..1710
                     /locus_tag="PW109_002685"
     CDS             301..1710
                     /locus_tag="PW109_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512428.1"
                     /translation="MNHQNEKNIASNVLLISILGLSVLVGSVTADMVNPVLGVIGKEL
                     GGSEAQVSWVVSGVALVLSIAIPFYGRLSDFLNIKKLFTNGFLILTIGSLICIFAPNL
                     IILVLGRMFQGAGMAAIPVLSIVIISKIYPPGQRGRILGIIAGCIGVGTAGGPIFGGV
                     VGQLLGWQSLFWVTFVLGLIIVLGVQISMPKIESPDNNSHQNFDVLGGLLLGLTVGGF
                     LLGITLSEMYGLISIQTTTSFSISMIALIVLIYRVINVKNPFIPPVILKNRLYVSSIF
                     IVFLSMFAYVSMLVFIPLLVVEVNGLSTGQAGLILLSGGVAVAILSPIVGRLSDKVHP
                     KILLLVGLIIMGLSSLYMSFVAGASPVLLSIGSLGIGIAFAFINSPVNNVAVLALPKE
                     QVGVGTGLFQGAMYLGAGTGASLIGALLSMRHGVKASFNPFYTLTAPHYSDIFLTITC
                     IVLVALIVTLNISSRDLKQ"
     gene            complement(2040..>3398)
                     /locus_tag="PW109_002686"
     CDS             complement(2040..>3398)
                     /locus_tag="PW109_002686"
                     /inference="COORDINATES: protein motif:HMM:NF037897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512429.1"
                     /translation="LFDSRFKTLQQNQWEDILKWGTVIVPETSSYISDNPIQELDDMG
                     FNSLPLNDNHIIKENTTTEKRSKIDNLYHGSKEDFTEFKIPRFHKNGGTLGFGTYLTD
                     SLQRAKSYADNDGYLYTIELNDELQNSTPLNASKVTLTTNQVAKIIEKIAQKQIDEEE
                     YPYILSDWEEPTSETAMDAGNKKIAYNIAEKIIDDTDDLSIINGINNQLGGNDSGAET
                     LIPVLNEMGIHYAELDVEYYDNSKEYVVFNPKDIKIVNQKLTKPEQSKKFVSDVDIKS
                     LEEKAIKTIAKSELIVKANNPVEAMQKLYNNDLKSAIKASFILYDLPEDKIDSSKKNV
                     TWEDLVNIEKSYNVFSYYQNNLYGSELVEPDLNNPYQENTKILDNKATQKQPALSDQY
                     KALHDKLKEPGANKQQIQEQLNKVKIEISQKTQQQLKDFAKANPDLKQPRVKKEQQAL
                     QR"
BASE COUNT          970 a          512 c          578 g         1338 t
ORIGIN      
        1 gtatagatta atataagagg aggataaaaa tgatggaaat ggtgtttacc ctttataaaa
       61 caattaaagt ttcagactat ctcaaagttg agggacaagt atgtactttt ttaaacagaa
      121 taccaaaatc cagacttttt actataatcc ttacgtaaaa aagtaaaata ttgatttatg
      181 taacaaaagt ctgatcttaa gattaggttt tttatttgga taattgagag gtgaaattga
      241 tggttaaagc agtcgattta gccttctaaa atgagcattt aaattttagg aggctgtaaa
      301 atgaatcatc aaaatgaaaa aaatatagca tcaaatgtgt tgttaattag tatcttaggc
      361 ttgtctgttt tagtgggttc agttactgca gatatggtta atcctgttct cggtgtgatt
      421 ggaaaagaat taggtgggtc tgaggcgcaa gttagttggg tcgtaagtgg tgttgcctta
      481 gttctatcta tagcaattcc cttttatgga cgtttatctg attttttaaa tattaaaaaa
      541 ctctttacta acggatttct aatattaact ataggaagtt taatatgtat attcgcgccc
      601 aatttaatta ttttagtatt gggtagaatg tttcaaggag ctggcatggc agccatacct
      661 gtgctatcca ttgttataat atctaaaatt tatccgcctg gacagagagg aagaatttta
      721 ggtattattg ctggttgtat tggcgttggc actgcaggag gcccaatatt tggcggcgtt
      781 gtaggacaat tattaggttg gcaatcatta ttttgggtca cttttgttct gggtttaatt
      841 atagttctag gtgttcaaat atcaatgcct aaaatagaat caccagataa taacagtcat
      901 caaaattttg atgttttagg tggactatta ttaggattaa ccgtaggtgg ttttttactt
      961 ggtattacgc tttcagaaat gtatggtttg atttctatac aaacaaccac aagtttttct
     1021 atatctatga tagctttaat agtgttaata tatcgcgtga ttaatgttaa aaatcctttt
     1081 atccctccag ttatattgaa gaaccgttta tatgtaagct caattttcat tgtatttctt
     1141 tcaatgtttg cttatgtctc tatgcttgtt tttattccat tattagttgt tgaggttaat
     1201 gggttaagta ctggacaggc aggcttaata ttactttctg gtggtgtcgc tgttgcaatc
     1261 ctttcaccaa tagtgggaag attatctgac aaagtgcatc ctaaaatact attattagtt
     1321 ggactaatta ttatgggctt atcttcttta tatatgagct tcgtagcagg cgcatcacct
     1381 gtattattat ctatcgggag tttagggata ggtatcgctt ttgcatttat taactctcca
     1441 gtgaataatg ttgcagtact tgctttacct aaagaacaag ttggtgtagg tacggggttg
     1501 tttcaaggtg caatgtatct tggggcagga acaggtgcct cactaatagg ggctttattg
     1561 tcaatgagac atggggttaa agcatctttt aatccttttt atactttaac tgccccgcac
     1621 tattctgata tatttttaac cattacgtgc atcgtgttag ttgctttaat tgttacttta
     1681 aatataagta gtagggattt aaagcaataa aaccttttta ttacacagat atacatctgt
     1741 ttttaaatat tattcagaaa ctaaattgtt aatagcaaga acaactaaat aattttaaaa
     1801 aacagggctt attaacaagc cctgttttgc attttagcca agaaaataac cagtttctgt
     1861 acactatcta actatcactt ttttcaattc ataatattgt atagcattcc atacgtttca
     1921 ttttctcatt tattaagtaa aatcattcta atttagcaag taaagtcaca acggtcttag
     1981 aatgcgcctc tcaaacgtca aaatacaaaa aagcgcctat aaataggcgc tttttatgat
     2041 tatcgttgca aagcttgctg ttcttttttt actcttggtt gcttcaaatc tgggttagct
     2101 tttgcaaagt cttttaattg ttgctgggtc ttttgactaa tttcgatttt aactttgttt
     2161 agttgttcct gtatttgttg tttatttgca cctggctctt ttaacttatc atgcaatgct
     2221 ttgtattgat ctgataaggc tggttgtttt tgtgtggctt tattatcaag aatcttagtg
     2281 ttttcctgat atggattgtt caaatcaggt tctacaagtt cactaccgta taagttgttt
     2341 tgataataac taaacacatt atatgatttt tctatgttga ctaaatcttc ccatgtaaca
     2401 ttctttttac tgctgtcgat tttatcttct ggtaaatcat ataatataaa gctggctttt
     2461 atagcgcttt ttaaatcatt gttataaagc ttttgcatag cttcaactgg gttgtttgct
     2521 ttaacaatta gttcagattt tgcgattgtt tttatagctt tttcttccaa ggattttata
     2581 tcaacatcac taacaaactt tttactttgt tctggttttg ttagtttctg attaacgatt
     2641 ttaatatcct ttggattaaa tacaacgtat tctttggaat tatcatagta ttcaacatct
     2701 aattctgcat agtgaatacc catttcattt aaaacaggaa ttagtgtttc tgctccacta
     2761 tcgttaccgc ctaattgatt gtttatgcca ttgataatgc ttaaatcatc agtatcatca
     2821 ataatctttt cagctatatt ataagcaatt tttttgttgc ctgcgtccat tgctgtttca
     2881 ctagttggtt cttcccaatc ggacaagatg taggggtatt cttcttcatc aatctgtttc
     2941 tgtgctatct tttcaattat tttagcgact tgattagttg ttaatgtaac tttgctggcg
     3001 ttcaaaggtg tactattttg tagttcatca ttcaattcaa tagtatacaa atagccgtca
     3061 ttatctgcat aagatttagc tctttgcaag ctatcagtta gataagttcc aaagcctaat
     3121 gttccaccat ttttatgaaa tcttggtatt ttaaattcag taaaatcctc ttttgaccca
     3181 tgatataaat tgtctatttt acttcgcttt tcagttgtag tattttcttt aatgatgtga
     3241 ttatcattta aaggtaagct attgaatccc atatcatcta attcttgaat tgggttatcg
     3301 ctaatgtagc tacttgtttc tggtacaata actgtccccc attttaaaat atcttcccat
     3361 tggttctgtt gcaaagtttt aaatctacta tcaaataa
//
LOCUS       DANKHK010000247         3398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.156, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000247 DANKHK010000000
VERSION     DANKHK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            301..1710
                     /locus_tag="PW109_002687"
     CDS             301..1710
                     /locus_tag="PW109_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2512430.1"
                     /translation="MNHQNEKNIASNVLLISILGLSVLVGSVTADMVNPVLGVIGKEL
                     GGSEAQVSWVVSGVALVLSIAIPFYGRLSDFLNIKKLFTNGFLILTIGSLICIFAPNL
                     IILVLGRMFQGAGMAAIPVLSIVIISKIYPPGQRGRILGIIAGCIGVGTAGGPIFGGV
                     VGQLLGWQSLFWVTFVLGLIIVLGVQISMPKIESPDNNSHQNFDVLGGLLLGLTVGGF
                     LLGITLSEMYGLISIQTTTSFSISMIALIVLIYRVINVKNPFIPPVILKNRLYVSSIF
                     IVFLSMFAYVSMLVFIPLLVVEVNGLSTGQAGLILLSGGVAVAILSPIVGRLSDKVHP
                     KILLLVGLIIMGLSSLYMSFVAGASPVLLSIGSLGIGIAFAFINSPVNNVAVLALPKE
                     QVGVGTGLFQGAMYLGAGTGASLIGALLSMRHGVKASFNPFYTLTAPHYSDIFLTITC
                     IVLVALIVTLNISSRDLKQ"
     gene            complement(2040..>3398)
                     /locus_tag="PW109_002688"
     CDS             complement(2040..>3398)
                     /locus_tag="PW109_002688"
                     /inference="COORDINATES: protein motif:HMM:NF037897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2512431.1"
                     /translation="LFDSRFKTLQQNQWEDILKWGTVIVPETSSYISDNPIQELDDMG
                     FNSLPLNDNHIIKENTTTEKRSKIDNLYHGSKEDFTEFKIPRFHKNGGTLGFGTYLTD
                     SLQRAKSYADNDGYLYTIELNDELQNSTPLNASKVTLTTNQVAKIIEKIAQKQIDEEE
                     YPYILSDWEEPTSETAMDAGNKKIAYNIAEKIIDDTDDLSIINGINNQLGGNDSGAET
                     LIPVLNEMGIHYAELDVEYYDNSKEYVVFNPKDIKIVNQKLTKPEQSKKFVSDVDIKS
                     LEEKAIKTIAKSELIVKANNPVEAMQKLYNNDLKSAIKASFILYDLPEDKIDSSKKNV
                     TWEDLVNIEKSYNVFSYYQNNLYGSELVEPDLNNPYQENTKILDNKATQKQPALSDQY
                     KALHDKLKEPGANKQQIQEQLNKVKIEISQKTQQQLKDFAKANPDLKQPRVKKEQQAL
                     QR"
BASE COUNT          970 a          511 c          578 g         1339 t
ORIGIN      
        1 gtatagatta atataagagg aggataaaaa tgatggaaat ggtgtttacc ctttataaaa
       61 caattaaagt ttcagactat ctcaaagttg agggacaagt atgtactttt ttaaacagaa
      121 taccaaaatc cagacttttt actataatcc ttacgtaaaa aagtaaaata ttgatttatg
      181 taacaaaagt ctgatcttaa gattaggttt tttatttgga taattgagag gtgaaattga
      241 tggttaaagc agtcgattta gccttctaaa atgagcattt aaattttagg aggctgtaaa
      301 atgaatcatc aaaatgaaaa aaatatagca tcaaatgtgt tgttaattag tatcttaggc
      361 ttgtctgttt tagtgggttc agttactgca gatatggtta atcctgttct cggtgtgatt
      421 ggaaaagaat taggtgggtc tgaggcgcaa gttagttggg tcgtaagtgg tgttgcctta
      481 gttctatcta tagcaattcc cttttatgga cgtttatctg attttttaaa tattaaaaaa
      541 ctctttacta acggatttct aatattaact ataggaagtt taatatgtat attcgcgccc
      601 aatttaatta ttttagtatt gggtagaatg tttcaaggag ctggcatggc agccatacct
      661 gtgctatcta ttgttataat atctaaaatt tatccgcctg gacagagagg aagaatttta
      721 ggtattattg ctggttgtat tggcgttggc actgcaggag gcccaatatt tggcggcgtt
      781 gtaggacaat tattaggttg gcaatcatta ttttgggtca cttttgttct gggtttaatt
      841 atagttctag gtgttcaaat atcaatgcct aaaatagaat caccagataa taacagtcat
      901 caaaattttg atgttttagg tggactatta ttaggattaa ccgtaggtgg ttttttactt
      961 ggtattacgc tttcagaaat gtatggtttg atttctatac aaacaaccac aagtttttct
     1021 atatctatga tagctttaat agtgttaata tatcgcgtga ttaatgttaa aaatcctttt
     1081 atccctccag ttatattgaa gaaccgttta tatgtaagct caattttcat tgtatttctt
     1141 tcaatgtttg cttatgtctc tatgcttgtt tttattccat tattagttgt tgaggttaat
     1201 gggttaagta ctggacaggc aggcttaata ttactttctg gtggtgtcgc tgttgcaatc
     1261 ctttcaccaa tagtgggaag attatctgac aaagtgcatc ctaaaatact attattagtt
     1321 ggactaatta ttatgggctt atcttcttta tatatgagct tcgtagcagg cgcatcacct
     1381 gtattattat ctatcgggag tttagggata ggtatcgctt ttgcatttat taactctcca
     1441 gtgaataatg ttgcagtact tgctttacct aaagaacaag ttggtgtagg tacggggttg
     1501 tttcaaggtg caatgtatct tggggcagga acaggtgcct cactaatagg ggctttattg
     1561 tcaatgagac atggggttaa agcatctttt aatccttttt atactttaac tgccccgcac
     1621 tattctgata tatttttaac cattacgtgc atcgtgttag ttgctttaat tgttacttta
     1681 aatataagta gtagggattt aaagcaataa aaccttttta ttacacagat atacatctgt
     1741 ttttaaatat tattcagaaa ctaaattgtt aatagcaaga acaactaaat aattttaaaa
     1801 aacagggctt attaacaagc cctgttttgc attttagcca agaaaataac cagtttctgt
     1861 acactatcta actatcactt ttttcaattc ataatattgt atagcattcc atacgtttca
     1921 ttttctcatt tattaagtaa aatcattcta atttagcaag taaagtcaca acggtcttag
     1981 aatgcgcctc tcaaacgtca aaatacaaaa aagcgcctat aaataggcgc tttttatgat
     2041 tatcgttgca aagcttgctg ttcttttttt actcttggtt gcttcaaatc tgggttagct
     2101 tttgcaaagt cttttaattg ttgctgggtc ttttgactaa tttcgatttt aactttgttt
     2161 agttgttcct gtatttgttg tttatttgca cctggctctt ttaacttatc atgcaatgct
     2221 ttgtattgat ctgataaggc tggttgtttt tgtgtggctt tattatcaag aatcttagtg
     2281 ttttcctgat atggattgtt caaatcaggt tctacaagtt cactaccgta taagttgttt
     2341 tgataataac taaacacatt atatgatttt tctatgttga ctaaatcttc ccatgtaaca
     2401 ttctttttac tgctgtcgat tttatcttct ggtaaatcat ataatataaa gctggctttt
     2461 atagcgcttt ttaaatcatt gttataaagc ttttgcatag cttcaactgg gttgtttgct
     2521 ttaacaatta gttcagattt tgcgattgtt tttatagctt tttcttccaa ggattttata
     2581 tcaacatcac taacaaactt tttactttgt tctggttttg ttagtttctg attaacgatt
     2641 ttaatatcct ttggattaaa tacaacgtat tctttggaat tatcatagta ttcaacatct
     2701 aattctgcat agtgaatacc catttcattt aaaacaggaa ttagtgtttc tgctccacta
     2761 tcgttaccgc ctaattgatt gtttatgcca ttgataatgc ttaaatcatc agtatcatca
     2821 ataatctttt cagctatatt ataagcaatt tttttgttgc ctgcgtccat tgctgtttca
     2881 ctagttggtt cttcccaatc ggacaagatg taggggtatt cttcttcatc aatctgtttc
     2941 tgtgctatct tttcaattat tttagcgact tgattagttg ttaatgtaac tttgctggcg
     3001 ttcaaaggtg tactattttg tagttcatca ttcaattcaa tagtatacaa atagccgtca
     3061 ttatctgcat aagatttagc tctttgcaag ctatcagtta gataagttcc aaagcctaat
     3121 gttccaccat ttttatgaaa tcttggtatt ttaaattcag taaaatcctc ttttgaccca
     3181 tgatataaat tgtctatttt acttcgcttt tcagttgtag tattttcttt aatgatgtga
     3241 ttatcattta aaggtaagct attgaatccc atatcatcta attcttgaat tgggttatcg
     3301 ctaatgtagc tacttgtttc tggtacaata actgtccccc attttaaaat atcttcccat
     3361 tggttctgtt gcaaagtttt aaatctacta tcaaataa
//
LOCUS       DANKHK010000248         2925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.168, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000248 DANKHK010000000
VERSION     DANKHK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            <1..138
                     /locus_tag="PW109_002689"
     CDS             <1..138
                     /locus_tag="PW109_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2512432.1"
                     /translation="KPKKSSRSEEILIKTLKRRVMELEKENKKLQNQIQKLYGDLYNK
                     E"
     gene            289..1071
                     /locus_tag="PW109_002690"
     CDS             289..1071
                     /locus_tag="PW109_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL2512433.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1196..1927)
                     /gene="erm(A)"
                     /locus_tag="PW109_002691"
     CDS             complement(1196..1927)
                     /gene="erm(A)"
                     /locus_tag="PW109_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL2512434.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNINKQDNVIEIGSGKGHF
                     TKELVKMSRWVDSIEIDEDLCQVTQKVVKPFQNIKVIHTDILKFNFPKNKDYKIFGNI
                     PFNISTDIVKKIAFESNSKYSYLIVENGFAKRLQNTKRALGLLLMVELDIRVLKKVPR
                     AYFHPKPNVDSVLIVLERHQPLILKKDYKEYQSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFQ"
     gene            2318..>2925
                     /locus_tag="PW109_002692"
     CDS             2318..>2925
                     /locus_tag="PW109_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2512435.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLP"
BASE COUNT         1005 a          428 c          529 g          963 t
ORIGIN      
        1 aaacccaaga aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta
       61 atggaattag aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat
      121 ctgtataata aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg
      181 ctaacggtca gctttattga acagtaattt aagtatatgt ccaatctagg gtaagtaaat
      241 tgagtatcaa tataaacttt atatgaacat aatcaacgag gtgaaatcat gagcaatttg
      301 attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt
      361 ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt
      421 aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa
      481 aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga
      541 ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa
      601 agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa
      661 ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct
      721 ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga
      781 gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta
      841 attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat
      901 gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt actggatata
      961 gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg
     1021 aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt
     1081 attgcaataa caggtgccta cttttaaaac aaccgattta ttaataaata ttgaaaattt
     1141 ttaggaagaa taaagcgttc tcttgtgaaa ttagagaacg ctttattact ttaatttact
     1201 gaaacaattt gtaactattg aaaatagaaa gaaattgttc cttcgatagt ttattaatat
     1261 tagtgacatt tgcatgcttc aaagcctgtc ggaattggtt tttagtaaaa agaacgcgat
     1321 attcacggtt tacccactta taaacaaaag attgatactc tttgtagtcc ttctttaaaa
     1381 tcaatggttg atgtcgttca agaacaatca atacagagtc tacattcggt ttagggtgaa
     1441 aatatgctcg tggcactttt ttgagaactc ttatgtccaa ttccaccatc aatagcaaac
     1501 ctaaagctcg tttcgtattt tgtaatcttt ttgcaaaacc attctccaca ataaggtaac
     1561 tatatttcga attgctttca aaagcaattt ttttgacaat atcagtactg atgttaaagg
     1621 ggatattacc aaatatttta tagtctttgt ttttggggaa gttaaatttc agaatatccg
     1681 tatggataac ttttatattc tgaaaaggct tcacgacttt ttgggtgact tgacacaagt
     1741 cctcatcaat ttctatagaa tccacccacc gactcatttt gacaagttct ttggtaaaat
     1801 gaccttttcc tgatccaatt tctattacat tatcttgttt atttatattc gtatgattca
     1861 atatctcttt tacatgcttt ttagaagtaa taaaattttg agtgtccttt gggtttttct
     1921 ggttcattat aaccttctcc ttgccatttt tttcattatg aatgagataa agtaatatct
     1981 actactgcga tactagtgca catacttgaa acctccttac gtaactgatt atatcatttc
     2041 tgtttacttt ttagaataaa gctctatatg taattttgca cttgacactt cagttggacg
     2101 ttttcttcaa aataatatgt tttgaaactt gataagagaa gccactgtct ttactaaaaa
     2161 gtaaaggctg cgataaagga cagtgacttt aataaagttg cactgttgta ttgtctttgg
     2221 ataaaattgt ctaaagggaa gttttatccc caacgaaaaa acatctaaag tggtaagtaa
     2281 tgaaagaatg tcgataaagt aggaatgggg gatctttttg aatacaatag aatcacagtt
     2341 tgataaggtt gcagaagatt acgatttcgt gaatgagctt ttgaatgatt attcattctt
     2401 tgtgtctaat atgtctccaa agaaaggcag agcattagat atcggatgtg gctcgggttt
     2461 gttagtggag aaattagcaa gttattatga tgaagtggta gggattgata tttctaatca
     2521 aatgctcgat cttgccaaat ctaaacgtca actaacaaat acggtctatc tgaatatgaa
     2581 tgcagaacaa cttaatttta atgagaagtt tgattttatt gtaagccgaa caacctttca
     2641 tcatttggat gatatagcca gcgtaataca gcaaatgaag gaactgttga atgaggaagg
     2701 aagaatagtt attcttgata atgtatctga agtcgagaca ccgcctacct atgtttataa
     2761 attaggggca atccaagagt ttttaccgca ttgtttcaaa tttggaataa aaaatgcgat
     2821 tagaatttat aatcacaaca catctaaatc atggcttgag catttagctt cagataagta
     2881 cctatctgaa caaaattatt acgatttata tgaaaagttg ttgcc
//
LOCUS       DANKHK010000249         2588 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.173, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000249 DANKHK010000000
VERSION     DANKHK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            944..2572
                     /gene="poxtA"
                     /locus_tag="PW109_002693"
     CDS             944..2572
                     /gene="poxtA"
                     /locus_tag="PW109_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein PoxtA"
                     /protein_id="HDL2512436.1"
                     /translation="MKGKNMNLAFGLEEIYEDAEFQIGDLDKVGIVGVNGAGKTTLFR
                     LLLGELELDNGSLTSGNARIGYLPQEIVLEDEDITVWDFLFEGRPIKKYEQELEEIYK
                     KLETAVNAEQEALLARMGTLQERLEYFDFYEAETILLEFADKMSIDAELYHRPMRELS
                     GGQKSKMAFARLLYSKPEILLLDEPTNHLDVSTKDFVIKYLKNYRGSVLIISHDIDFL
                     NRIINKIMYINKATHKISVYDGDYYIYKKKYAEEQRIREMAIVQQEKEIKELSDFVQK
                     AKQASQTNHHLKRMGQERALRLDKKRGELQKRNRLYKRVKMDIRPKREGAQVPLEVEN
                     ITFHYSGYPTLYQNLSFQINGRERFLVVGENGVGKSTLLKLMMGILSPDEGCIRFNQK
                     TDIAYYAQELEQLDENKTVIDNVESEGYTPWQIRAVLSNFLFYDDDVNKKVSVLSPGE
                     KARVALCKILLQKANLLILDEPTNHLDPETQKIIGGNFNLFEGTIIAVSHNPSFVEQI
                     GISRMLILPSGRIEPYSRELLEYYYEINGSVAKF"
BASE COUNT          822 a          455 c          563 g          748 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca catgacaaat gcaagctttg
       61 gaatttacat tattcattat cctgttgttg tgtgggtatg ctacttgctt tacagttacc
      121 tcaacttacc gatgatattt atctatattc ttgctttagg gcttgaactg atactgacac
      181 cgttaattta tgagctgttt aaaaggattc ctgtagttcg ctttttagta ttggggataa
      241 aaaaatgagc atttcttact atcctattaa acgagtatag ctatcctatc ggcagtagcg
      301 ttgtctatgg ttatggcttg cctgcatatt aaaaaagggc aaaagaaaaa cagaggattt
      361 tacccctctg tttcctcttt tgcgttacat attcagttta cagttgtaca atacttggtc
      421 tataaacaaa ctgtaaagtg agcagcttag atgagctatt gacatatgca ctcaagctct
      481 ctataattaa aatataaaat aagagagggt gagcacagtg ttattcggga agttttgcat
      541 tgattttggc tctacacaac aatataagac agagcattgt gtggagccca atcagataaa
      601 atgtttattt gagaggcagg gctatttaaa tcagaaattt gtgatttaag agccttattt
      661 gtgttgcctt tcaataagca aactcaatga ttggatatgt ggccgtagac aatgttttgt
      721 ctacggcctt ttgctgtcta tatttcatct atgttattgg catagtgtct gaaaaagcag
      781 agggctacgt ctatgggacg tattgctacc tctgctttta ttttttaaat gatgatatca
      841 gagaagaaaa tatatccccg cacaggagat aatcaagcaa ggaaaactgt aagccataaa
      901 atgatggctt gcagtatgta tgacgatgga ggaatgatac aagatgaaag gtaaaaatat
      961 gaatttagcc tttgggttgg aagaaattta tgaggatgct gagtttcaaa tcggagattt
     1021 ggataaggtc ggtattgtcg gcgtgaacgg agccggaaag accaccttgt tccgcctgct
     1081 gttgggagaa cttgaacttg ataatggttc actgaccagt ggaaatgccc gtattggtta
     1141 tctcccacag gaaattgtct tggaagatga ggatattacc gtttgggatt tcctttttga
     1201 gggacgtccg attaaaaagt atgagcagga attggaagaa atctataaaa agcttgaaac
     1261 cgcagtcaat gcagagcagg aagcactgct tgcccgaatg ggaacattgc aagaacgctt
     1321 ggagtatttc gacttctatg aggcagaaac aattctgttg gagtttgcag ataaaatgag
     1381 cattgatgca gaattatatc atcgtccgat gagagagctt tcaggcggac aaaaatccaa
     1441 aatggcattt gccagactac tatattcaaa accggaaatt ctattgttgg atgagcctac
     1501 caaccattta gatgtcagca caaaggattt tgttataaaa tacttaaaga attatagggg
     1561 ttcggtactg attatcagcc atgatattga ttttctaaat cggattatca acaaaattat
     1621 gtacatcaac aaagctaccc ataaaatatc tgtttatgat ggagactact acatctacaa
     1681 gaaaaagtat gcagaggaac agcggattcg tgaaatggcg attgtacagc aggaaaaaga
     1741 aataaaggag ctttccgatt ttgtacaaaa agcaaaacaa gccagtcaga ccaatcatca
     1801 cctcaaacga atgggtcaag agcgagcctt gcggcttgat aaaaagcgtg gagagctgca
     1861 aaagagaaat cgactgtaca agcgtgtgaa gatggatatt cgccccaagc gtgaaggggc
     1921 acaagttccc ttagaggtgg aaaatatcac cttccactat tcggggtatc ccacccttta
     1981 tcagaacctt tcctttcaga ttaacggaag agaacgattt cttgtggtgg gtgaaaacgg
     2041 tgtcggtaaa tccaccttat tgaaattgat gatgggtatt ctcagtccag atgaaggatg
     2101 cattcgcttt aaccagaaaa ctgatattgc atattatgca caggaactcg aacagcttga
     2161 tgaaaacaaa acggtcattg acaatgtgga gtctgaagga tatacaccgt ggcaaatcag
     2221 agccgtactg agcaacttcc tgttttatga tgacgatgta aacaagaaag tatctgtgct
     2281 gtcccctgga gaaaaagcaa gggttgccct ttgcaaaatc ctattacaga aagccaatct
     2341 tttgatactg gacgagccga ccaaccacct tgacccagaa acgcagaaaa tcattggcgg
     2401 caacttcaat ttgtttgagg gaaccattat tgccgttagc cataacccat cctttgtgga
     2461 acaaatcgga attagccgta tgcttatttt gcccagcggt cgaattgaac cctattcccg
     2521 tgagctgctt gagtattatt atgaaatcaa cggttctgtt gcaaagtttt aaatctacta
     2581 tcaaataa
//
LOCUS       DANKHK010000250         1020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538943-rid17233433.guided.221, whole genome shotgun
            sequence.
ACCESSION   DANKHK010000250 DANKHK010000000
VERSION     DANKHK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538943
            Sequence Read Archive: ERR10688506
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638260.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638260.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:44:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,694
            CDSs (total)                      :: 2,634
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 39 of 83
            Pseudo Genes (incomplete)         :: 31 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 5 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538943-rid17233433.guided.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Aspirate, ventricle (stomach)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-01-09"
     gene            118..855
                     /gene="erm(B)"
                     /locus_tag="PW109_002694"
     CDS             118..855
                     /gene="erm(B)"
                     /locus_tag="PW109_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2512437.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          387 a          173 c          150 g          310 t
ORIGIN      
        1 agattaattc ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa
       61 acaaatcgtt taacttctgt atttgtttat agatgcaatc acttcaggag tgattacatg
      121 aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata
      181 ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg
      241 catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt
      301 catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac
      361 caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt tgggaatatt
      421 ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca tgcgtctgac
      481 atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta
      541 gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc
      601 tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat
      661 gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa
      721 tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac
      781 aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt
      841 aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa
      901 agggaatgta gataaattat taggtatagg gcacctctaa taactaccaa ttaattcacc
      961 tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc tatttcttat cgatacaaat
//
